Lsi09G010720 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi09G010720
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Cysteine protease ATG4B
Location	chr09 : 16640654 .. 16642756 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTGAGGTTATCGAGAACTTGGTGATTCTTTATGTGGAGGACCAGGAGGTTGATGGAGTTGTGGTGAGGGAGTGGTGAGAGGAAGAGGTGTACTTCAAGGCTTGCGATAATGTCAATGGGTTATACTACCATTGAACAAGTGCTCCTCTGTTTGCCTTTTCAGGGGAAGGGTAATTTTTTGTGGCAAGCTAGTGTCTGCTATTTTATGGTGAGTTTAGTTCGAGAGGAACAATAGGGTTTTTAAAGAGGCGGTGTGGTCTTGTGATGAGGTTTGGGAAATGGTGAGGGTTCAATGCATCCCTGTGGGCTTTTGGTCGCTAACCTTAATTTGATTTTATTGGATTGGAGTCCTTTTTTTGTAGTTTCTTGGGGTCTCCTTGTTTTGTTGGGATTTTTTTCCCCTCTTTTTGTATGCCTTTTACATTATTTCATTTCTATCATAATGGAGAGATCATGAGCCAGCGATATTTTGGTTTCTAGTGCTCCAGCTAGATCTTTGAATCTGGAACAAGCATAGGTACATTTTTGACACTTGAATTAAAGTCTGAAATGTTTTACAGTACCCCTTAGAACTTACAGGCGCTGTGATCCATTGTAGGCATGTGGACTCAGCTAATAAAATGACAAAATGTACAGAATACTATGATGTAGTACCTTTTTTTCTTTTAAAAGAAAAAAAAGCTTAATTATCCAGAACAAATGTCTCCCTGAGCTGTAGGATAATTTTTACATTTTTGTAAATAAATCTTTAGTTGTAATGATAAAATATTCAATCCCTCAAATATATTTTGTTTGGTTTTGGATGTACTCTATAACATTTGTTACAAACTATGGCATCCTGAAAAAAGAAAAGTTTTTTACATCATGATTGCGGTTGCTAACTTTGTTTCGTTATTTGAGAATTGCTATGCGTAAGAAAATTCACATGTTAACTTTTGTTTTATCGCATTGTGTTTTGGTTTTGATTCTTCAAACAAGATTGCTTCTGTTTTCTACGCTGAATGTTTATTGTCGCGGTTTCCTTGTTCATGTCAATTTACAGAATGGGATAGGTAATGACTGTCTAACAATGTTTTCATTTTAGGTAGTCAATATTGATAAGGATGACCTAGAGGCTGATACTTCCTCTTATCACTGCAAGTGAGTTTTTCCATATCATAAATGTCTTGTGGTTTACTGTTTTTCATTTTTCGTCTTTCATTTTTGTTTGGGTTCTCATATATATATCTCTGTACATTGTTTTCTGTAGTGTCATCCGGCACATCCCCTTAGAATCCATAGATCCTTCTCTAGCAATTGGGTTTTATTGTCGAGACAAAGGTTTGCACACCCCTCTAGCTTCATAATTGTGGATGTGAGCTTGATCGATTCCTTTCATGTTGTAAAACCTAATCTTGATTTCCTGTTTTGCTCCCTCGTTTTTTCTTTTGTGTTCTTTTTTCCTGGTGCCAGATGATTTCGACAATTTCTGTTATCGGGCATCGAAGTTGGCAGACGAGTCGGATGGAGCTCCATTATTTACAGTTGCTGAAACACATTCCACGAATTCAGGGAGACACGGCAGTGCATTGAATGACTGTAGTAGATTAGTGGAGGATACCGATGGCGTGGTGCACATGCCGAACGAAGAGGAGGCGCATGAGGACGACTGGCAATTTCTTTGAAGAAAATAGAACCGGTTTTGGAATCTGGGGGTGGGTTGGTATTCTTCCTTAATTACTTCTCTAGGCTTCCAAGTTCCCTTGAGCTTTCTGGCTAATATGATTGAAATGGATGGGGAGTGATGCCCAATATGTCAAATGTTTTAGTTTATTTAGTGTTAATTGTGCAATAGGCGTTGAGTTTATTTGTTGAATTTGTTTATTCTTGGTCCACTTTCACGTGAAATTAGCCTACATTCGTGTGTATAATAAGCAGGTTCAATTCAAATTCATAAATGAATTATAAATGCTCTGATTCTATGAAAAATTTAGCTTACCATTCTTAACCGTTTACGGCCACCATGGCCACCGCTGCACAACCTCCTCTACCGATCTTCTCCCCTCCATCAATTCTTATTGATCAAAGTAACATTTCAATTGCAATTGACGAATATCCCAG

mRNA sequence

ATGGTTGAGGTTATCGAGAACTTGGTGATTCTTTATGTGGAGGACCAGGAGGTTGATGGAGTTGTGGTAGTCAATATTGATAAGGATGACCTAGAGGCTGATACTTCCTCTTATCACTGCAATGTCATCCGGCACATCCCCTTAGAATCCATAGATCCTTCTCTAGCAATTGGGTTTTATTGTCGAGACAAAGATGATTTCGACAATTTCTGTTATCGGGCATCGAAGTTGGCAGACGAGTCGGATGGAGCTCCATTATTTACAGTTGCTGAAACACATTCCACGAATTCAGGGAGACACGGCAGTGCATTGAATGACTGTAGTAGATTAGTGGAGGATACCGATGGCGTGGTGCACATGCCGAACGAAGAGGAGGCGCATGAGGACGACTGGCAATTTCTTTGAAGAAAATAGAACCGGTTTTGGAATCTGGGGGTGGGTTGGTATTCTTCCTTAATTACTTCTCTAGGCTTCCAAGTTCCCTTGAGCTTTCTGGCTAATATGATTGAAATGGATGGGGAGTGATGCCCAATATGTCAAATGTTTTAGTTTATTTAGTGTTAATTGTGCAATAGGCGTTGAGTTTATTTGTTGAATTTGTTTATTCTTGGTCCACTTTCACGTGAAATTAGCCTACATTCGTGTGTATAATAAGCAGGTTCAATTCAAATTCATAAATGAATTATAAATGCTCTGATTCTATGAAAAATTTAGCTTACCATTCTTAACCGTTTACGGCCACCATGGCCACCGCTGCACAACCTCCTCTACCGATCTTCTCCCCTCCATCAATTCTTATTGATCAAAGTAACATTTCAATTGCAATTGACGAATATCCCAG

Coding sequence (CDS)

ATGGTTGAGGTTATCGAGAACTTGGTGATTCTTTATGTGGAGGACCAGGAGGTTGATGGAGTTGTGGTAGTCAATATTGATAAGGATGACCTAGAGGCTGATACTTCCTCTTATCACTGCAATGTCATCCGGCACATCCCCTTAGAATCCATAGATCCTTCTCTAGCAATTGGGTTTTATTGTCGAGACAAAGATGATTTCGACAATTTCTGTTATCGGGCATCGAAGTTGGCAGACGAGTCGGATGGAGCTCCATTATTTACAGTTGCTGAAACACATTCCACGAATTCAGGGAGACACGGCAGTGCATTGAATGACTGTAGTAGATTAGTGGAGGATACCGATGGCGTGGTGCACATGCCGAACGAAGAGGAGGCGCATGAGGACGACTGGCAATTTCTTTGA

Protein sequence

MVEVIENLVILYVEDQEVDGVVVVNIDKDDLEADTSSYHCNVIRHIPLESIDPSLAIGFYCRDKDDFDNFCYRASKLADESDGAPLFTVAETHSTNSGRHGSALNDCSRLVEDTDGVVHMPNEEEAHEDDWQFL

Homology

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BLAST of Lsi09G010720 vs. Swiss-Prot
Match: ATG4_MEDTR (Cysteine protease ATG4 OS=Medicago truncatula GN=ATG4 PE=3 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 2.2e-29
Identity = 71/131 (54.20%), Postives = 91/131 (69.47%), Query Frame = 1


Query: 5   IENLVILYVEDQEVDGVVVVNIDKDDLEADTSSYHCNVIRHIPLESIDPSLAIGFYCRDK 64
           ++N    Y++  EV  VV  NI  D  E +TSSYHCN+ RH+PL+SIDPSLAIGFYCRDK
Sbjct: 360 VQNDKAFYLDPHEVKPVV--NITGDTQEPNTSSYHCNISRHMPLDSIDPSLAIGFYCRDK 419

Query: 65  DDFDNFCYRASKLADESDGAPLFTVAETHS-TNSGRHGSALNDCSRLVEDTDGVVHMPNE 124
           DDFD+FC RA+KLA+ES+GAPLFTVA++ S        S   D +R  ED    +++ N 
Sbjct: 420 DDFDDFCSRATKLAEESNGAPLFTVAQSRSLPMQVTSNSVSGDDTRFEEDDSLSMNLVN- 479

Query: 125 EEAHEDDWQFL 135
           +  +EDDWQFL
Sbjct: 480 DAGNEDDWQFL 487

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BLAST of Lsi09G010720 vs. Swiss-Prot
Match: ATG4A_ARATH (Cysteine protease ATG4a OS=Arabidopsis thaliana GN=ATG4A PE=2 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 5.0e-29
Identity = 67/124 (54.03%), Postives = 84/124 (67.74%), Query Frame = 1


Query: 12  YVEDQEVDGVVVVNIDKDDLEADTSSYHCNVIRHIPLESIDPSLAIGFYCRDKDDFDNFC 71
           Y++  EV  VV VN  K+  + DTSSYHCNV+R++PLES+DPSLA+GFYCRDKDDFD+FC
Sbjct: 362 YLDPHEVQQVVTVN--KETPDVDTSSYHCNVLRYVPLESLDPSLALGFYCRDKDDFDDFC 421

Query: 72  YRASKLADESDGAPLFTVAETHST-NSGRHGSALNDCSRLVEDTDGVVHMPNEEEAHEDD 131
            RA KLA+ES+GAPLFTV +TH+  N   +G A +D                 E+  EDD
Sbjct: 422 LRALKLAEESNGAPLFTVTQTHTAINQSNYGFADDD----------------SEDEREDD 467

Query: 132 WQFL 135
           WQ L
Sbjct: 482 WQML 467

[Close]

BLAST of Lsi09G010720 vs. Swiss-Prot
Match: ATG4B_ARATH (Cysteine protease ATG4b OS=Arabidopsis thaliana GN=ATG4B PE=1 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 1.0e-26
Identity = 60/123 (48.78%), Postives = 79/123 (64.23%), Query Frame = 1


Query: 12  YVEDQEVDGVVVVNIDKDDLEADTSSYHCNVIRHIPLESIDPSLAIGFYCRDKDDFDNFC 71
           Y++  +V  VV V   K++ + DTSSYHCN +R++PLES+DPSLA+GFYC+ KDDFD+FC
Sbjct: 366 YLDPHDVQQVVTVK--KENQDVDTSSYHCNTLRYVPLESLDPSLALGFYCQHKDDFDDFC 425

Query: 72  YRASKLADESDGAPLFTVAETHSTNSGRHGSALNDCSRLVEDTDGVVHMPNEEEAHEDDW 131
            RA+KLA +S+GAPLFTV ++H           NDC      +          E HEDDW
Sbjct: 426 IRATKLAGDSNGAPLFTVTQSHRR---------NDCGIAETSSSTETSTEISGEEHEDDW 477

Query: 132 QFL 135
           Q L
Sbjct: 486 QLL 477

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BLAST of Lsi09G010720 vs. Swiss-Prot
Match: ATG4A_ORYSI (Cysteine protease ATG4A OS=Oryza sativa subsp. indica GN=ATG4A PE=3 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 8.2e-24
Identity = 58/125 (46.40%), Postives = 82/125 (65.60%), Query Frame = 1


Query: 10  ILYVEDQEVDGVVVVNIDKDDLEADTSSYHCNVIRHIPLESIDPSLAIGFYCRDKDDFDN 69
           +LY++  EV   + V+I  D+LEADTSSYHC+ +R + L+ IDPSLAIGFYCRDKDDFD+
Sbjct: 353 VLYLDPHEVQ--LAVDIAADNLEADTSSYHCSTVRDLALDLIDPSLAIGFYCRDKDDFDD 412

Query: 70  FCYRASKLADESDGAPLFTVAETHSTNSGRHGSALNDCSRLVEDTDGVVHMPNEEEAHED 129
           FC RAS+L D+++GAPLFTV ++   +   +    +    +  D   V  +    E  E+
Sbjct: 413 FCSRASELVDKANGAPLFTVMQSVQPSKQMYNEESSSGDGM--DIINVEGLDGSGETGEE 472

Query: 130 DWQFL 135
           +WQ L
Sbjct: 473 EWQIL 473

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BLAST of Lsi09G010720 vs. Swiss-Prot
Match: ATG4A_ORYSJ (Cysteine protease ATG4A OS=Oryza sativa subsp. japonica GN=ATG4A PE=2 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 4.1e-23
Identity = 57/124 (45.97%), Postives = 81/124 (65.32%), Query Frame = 1


Query: 11  LYVEDQEVDGVVVVNIDKDDLEADTSSYHCNVIRHIPLESIDPSLAIGFYCRDKDDFDNF 70
           LY++  EV   + V+I  D+LEA TSSYHC+ +R + L+ IDPSLAIGFYCRDKDDFD+F
Sbjct: 355 LYLDPHEVQ--LAVDIAADNLEAGTSSYHCSTVRDLALDLIDPSLAIGFYCRDKDDFDDF 414

Query: 71  CYRASKLADESDGAPLFTVAETHSTNSGRHGSALNDCSRLVEDTDGVVHMPNEEEAHEDD 130
           C RAS+L D+++GAPLFTV ++   +   +    +    +  D+  V  +    E  E++
Sbjct: 415 CSRASELVDKANGAPLFTVVQSVQPSKQMYNEESSSGDGM--DSINVEGLDGSGETGEEE 474

Query: 131 WQFL 135
           WQ L
Sbjct: 475 WQIL 474

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BLAST of Lsi09G010720 vs. TrEMBL
Match: A0A0A0LK81_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G128640 PE=3 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 1.3e-52
Identity = 107/124 (86.29%), Postives = 112/124 (90.32%), Query Frame = 1


Query: 12  YVEDQEVDGVVVVNIDKDDLEADTSSYHCNVIRHIPLESIDPSLAIGFYCRDKDDFDNFC 71
           Y++  EV    VVNIDKDDLEADTSSYHCNVIRHIPLESIDPSLAIGFYCRDKDDFDNFC
Sbjct: 362 YLDPHEVQQ--VVNIDKDDLEADTSSYHCNVIRHIPLESIDPSLAIGFYCRDKDDFDNFC 421

Query: 72  YRASKLADESDGAPLFTVAETHSTNSGRHGSALNDCSRLVEDT-DGVVHMPNEEEAHEDD 131
           +RASKLA+ESDGAPLFTVAETHSTN GR  SALND SRLVED  DGVVHMPNEEE+HEDD
Sbjct: 422 HRASKLAEESDGAPLFTVAETHSTNPGRQSSALNDHSRLVEDDGDGVVHMPNEEESHEDD 481

Query: 132 WQFL 135
           WQFL
Sbjct: 482 WQFL 483

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BLAST of Lsi09G010720 vs. TrEMBL
Match: A0A061DVN9_THECC (Peptidase family C54 protein isoform 2 OS=Theobroma cacao GN=TCM_003165 PE=3 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 1.4e-33
Identity = 76/126 (60.32%), Postives = 96/126 (76.19%), Query Frame = 1


Query: 10  ILYVEDQEVDGVVVVNIDKDDLEADTSSYHCNVIRHIPLESIDPSLAIGFYCRDKDDFDN 69
           + Y++  +V   +VVN+ +D+ EADTSSYHC++IRHIPL+SIDPSLAIGF+CRDKDDFD+
Sbjct: 273 VFYLDPHDVQ--LVVNLSQDNQEADTSSYHCDIIRHIPLDSIDPSLAIGFFCRDKDDFDD 332

Query: 70  FCYRASKLADESDGAPLFTVAETHST-NSGRHGSALNDCSRLVEDTDGVVHMPNEEEAHE 129
           FC RASKLADES+GAPLFTVA+THS+     HG+AL+D   + ED    V    +   HE
Sbjct: 333 FCLRASKLADESNGAPLFTVAQTHSSFKPISHGNALDDTGEVREDDSLGVVPDMDGSIHE 392

Query: 130 DDWQFL 135
           DDWQ L
Sbjct: 393 DDWQLL 396

[Close]

BLAST of Lsi09G010720 vs. TrEMBL
Match: A0A061DMN0_THECC (Peptidase family C54 protein isoform 3 OS=Theobroma cacao GN=TCM_003165 PE=3 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 1.4e-33
Identity = 76/126 (60.32%), Postives = 96/126 (76.19%), Query Frame = 1


Query: 10  ILYVEDQEVDGVVVVNIDKDDLEADTSSYHCNVIRHIPLESIDPSLAIGFYCRDKDDFDN 69
           + Y++  +V   +VVN+ +D+ EADTSSYHC++IRHIPL+SIDPSLAIGF+CRDKDDFD+
Sbjct: 363 VFYLDPHDVQ--LVVNLSQDNQEADTSSYHCDIIRHIPLDSIDPSLAIGFFCRDKDDFDD 422

Query: 70  FCYRASKLADESDGAPLFTVAETHST-NSGRHGSALNDCSRLVEDTDGVVHMPNEEEAHE 129
           FC RASKLADES+GAPLFTVA+THS+     HG+AL+D   + ED    V    +   HE
Sbjct: 423 FCLRASKLADESNGAPLFTVAQTHSSFKPISHGNALDDTGEVREDDSLGVVPDMDGSIHE 482

Query: 130 DDWQFL 135
           DDWQ L
Sbjct: 483 DDWQLL 486

[Close]

BLAST of Lsi09G010720 vs. TrEMBL
Match: M5XJB4_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004885mg PE=3 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 4.4e-32
Identity = 76/127 (59.84%), Postives = 93/127 (73.23%), Query Frame = 1


Query: 11  LYVEDQEVDGVVVVNIDKDDLEADTSSYHCNVIRHIPLESIDPSLAIGFYCRDKDDFDNF 70
           LY++  EV     +NI +DDLEADT SYHCNVIRHIPL+SIDPSLAIGFYCRD+DDFD+F
Sbjct: 363 LYLDPHEVQPA--INIRRDDLEADTLSYHCNVIRHIPLDSIDPSLAIGFYCRDRDDFDDF 422

Query: 71  CYRASKLADESDGAPLFTVAETHSTNSGRHGSALNDCSRLVEDTDGVVHMPNEE---EAH 130
           C+RASKLAD S+GAPLFTV ++H+     + S + D S  V++ D  V  P  +    AH
Sbjct: 423 CFRASKLADGSNGAPLFTVTQSHNFPKPVNHSDVLDDSGGVQNDDSFVAPPISDADGSAH 482

Query: 131 EDDWQFL 135
           EDDWQ L
Sbjct: 483 EDDWQLL 487

[Close]

BLAST of Lsi09G010720 vs. TrEMBL
Match: A0A0D2SXT5_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_008G001300 PE=3 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 7.5e-32
Identity = 76/136 (55.88%), Postives = 97/136 (71.32%), Query Frame = 1


Query: 10  ILYVEDQEVDGVVVVNIDKDDLEADTSSYHCNVIRHIPLESIDPSLAIGFYCRDKDDFDN 69
           + Y++  +V  VV  N+  ++LEADTSSYHCN+IR+IPLES+DPSLAIGF+CRDKDDFD+
Sbjct: 363 VFYLDPHDVQPVV--NLSTENLEADTSSYHCNIIRYIPLESLDPSLAIGFFCRDKDDFDD 422

Query: 70  FCYRASKLADESDGAPLFTVAETHS---------TNSGRHGSALNDCSRLVE--DTDGVV 129
           FC+RASKLADES+GAPLFTVA+THS         T +   G  ++D  R++   D DG  
Sbjct: 423 FCFRASKLADESNGAPLFTVAQTHSVFKPINHGDTMANAGGDRMDDSVRVLPTGDVDG-- 482

Query: 130 HMPNEEEAHEDDWQFL 135
                  +HEDDWQFL
Sbjct: 483 ------NSHEDDWQFL 488

[Close]

BLAST of Lsi09G010720 vs. TAIR10
Match: AT2G44140.1 (AT2G44140.1 Peptidase family C54 protein)

HSP 1 Score: 128.6 bits (322), Expect = 2.8e-30
Identity = 67/124 (54.03%), Postives = 84/124 (67.74%), Query Frame = 1


Query: 12  YVEDQEVDGVVVVNIDKDDLEADTSSYHCNVIRHIPLESIDPSLAIGFYCRDKDDFDNFC 71
           Y++  EV  VV VN  K+  + DTSSYHCNV+R++PLES+DPSLA+GFYCRDKDDFD+FC
Sbjct: 362 YLDPHEVQQVVTVN--KETPDVDTSSYHCNVLRYVPLESLDPSLALGFYCRDKDDFDDFC 421

Query: 72  YRASKLADESDGAPLFTVAETHST-NSGRHGSALNDCSRLVEDTDGVVHMPNEEEAHEDD 131
            RA KLA+ES+GAPLFTV +TH+  N   +G A +D                 E+  EDD
Sbjct: 422 LRALKLAEESNGAPLFTVTQTHTAINQSNYGFADDD----------------SEDEREDD 467

Query: 132 WQFL 135
           WQ L
Sbjct: 482 WQML 467

[Close]

BLAST of Lsi09G010720 vs. TAIR10
Match: AT3G59950.1 (AT3G59950.1 Peptidase family C54 protein)

HSP 1 Score: 120.9 bits (302), Expect = 5.8e-28
Identity = 60/123 (48.78%), Postives = 79/123 (64.23%), Query Frame = 1


Query: 12  YVEDQEVDGVVVVNIDKDDLEADTSSYHCNVIRHIPLESIDPSLAIGFYCRDKDDFDNFC 71
           Y++  +V  VV V   K++ + DTSSYHCN +R++PLES+DPSLA+GFYC+ KDDFD+FC
Sbjct: 366 YLDPHDVQQVVTVK--KENQDVDTSSYHCNTLRYVPLESLDPSLALGFYCQHKDDFDDFC 425

Query: 72  YRASKLADESDGAPLFTVAETHSTNSGRHGSALNDCSRLVEDTDGVVHMPNEEEAHEDDW 131
            RA+KLA +S+GAPLFTV ++H           NDC      +          E HEDDW
Sbjct: 426 IRATKLAGDSNGAPLFTVTQSHRR---------NDCGIAETSSSTETSTEISGEEHEDDW 477

Query: 132 QFL 135
           Q L
Sbjct: 486 QLL 477

[Close]

BLAST of Lsi09G010720 vs. NCBI nr
Match: gi|659082126|ref|XP_008441684.1| (PREDICTED: cysteine protease ATG4-like isoform X1 [Cucumis melo])

HSP 1 Score: 217.6 bits (553), Expect = 1.3e-53
Identity = 109/124 (87.90%), Postives = 112/124 (90.32%), Query Frame = 1


Query: 12  YVEDQEVDGVVVVNIDKDDLEADTSSYHCNVIRHIPLESIDPSLAIGFYCRDKDDFDNFC 71
           Y++  EV    VVNIDKDDLEADTSSYHCNVIRHIPLESIDPSLAIGFYCRDKDDFDNFC
Sbjct: 362 YLDPHEVQQ--VVNIDKDDLEADTSSYHCNVIRHIPLESIDPSLAIGFYCRDKDDFDNFC 421

Query: 72  YRASKLADESDGAPLFTVAETHSTNSGRHGSALNDCSRLVE-DTDGVVHMPNEEEAHEDD 131
           YRASKLA+ESDGAPLFTVAETHSTNSGR  SALND SRLVE D DG VHMPNEEEAHEDD
Sbjct: 422 YRASKLAEESDGAPLFTVAETHSTNSGRQSSALNDHSRLVEDDADGAVHMPNEEEAHEDD 481

Query: 132 WQFL 135
           WQFL
Sbjct: 482 WQFL 483

[Close]

BLAST of Lsi09G010720 vs. NCBI nr
Match: gi|659082128|ref|XP_008441686.1| (PREDICTED: cysteine protease ATG4-like isoform X2 [Cucumis melo])

HSP 1 Score: 217.6 bits (553), Expect = 1.3e-53
Identity = 109/124 (87.90%), Postives = 112/124 (90.32%), Query Frame = 1


Query: 12  YVEDQEVDGVVVVNIDKDDLEADTSSYHCNVIRHIPLESIDPSLAIGFYCRDKDDFDNFC 71
           Y++  EV    VVNIDKDDLEADTSSYHCNVIRHIPLESIDPSLAIGFYCRDKDDFDNFC
Sbjct: 334 YLDPHEVQQ--VVNIDKDDLEADTSSYHCNVIRHIPLESIDPSLAIGFYCRDKDDFDNFC 393

Query: 72  YRASKLADESDGAPLFTVAETHSTNSGRHGSALNDCSRLVE-DTDGVVHMPNEEEAHEDD 131
           YRASKLA+ESDGAPLFTVAETHSTNSGR  SALND SRLVE D DG VHMPNEEEAHEDD
Sbjct: 394 YRASKLAEESDGAPLFTVAETHSTNSGRQSSALNDHSRLVEDDADGAVHMPNEEEAHEDD 453

Query: 132 WQFL 135
           WQFL
Sbjct: 454 WQFL 455

[Close]

BLAST of Lsi09G010720 vs. NCBI nr
Match: gi|449442361|ref|XP_004138950.1| (PREDICTED: cysteine protease ATG4-like isoform X2 [Cucumis sativus])

HSP 1 Score: 213.8 bits (543), Expect = 1.9e-52
Identity = 107/124 (86.29%), Postives = 112/124 (90.32%), Query Frame = 1


Query: 12  YVEDQEVDGVVVVNIDKDDLEADTSSYHCNVIRHIPLESIDPSLAIGFYCRDKDDFDNFC 71
           Y++  EV    VVNIDKDDLEADTSSYHCNVIRHIPLESIDPSLAIGFYCRDKDDFDNFC
Sbjct: 362 YLDPHEVQQ--VVNIDKDDLEADTSSYHCNVIRHIPLESIDPSLAIGFYCRDKDDFDNFC 421

Query: 72  YRASKLADESDGAPLFTVAETHSTNSGRHGSALNDCSRLVEDT-DGVVHMPNEEEAHEDD 131
           +RASKLA+ESDGAPLFTVAETHSTN GR  SALND SRLVED  DGVVHMPNEEE+HEDD
Sbjct: 422 HRASKLAEESDGAPLFTVAETHSTNPGRQSSALNDHSRLVEDDGDGVVHMPNEEESHEDD 481

Query: 132 WQFL 135
           WQFL
Sbjct: 482 WQFL 483

[Close]

BLAST of Lsi09G010720 vs. NCBI nr
Match: gi|778668355|ref|XP_011649086.1| (PREDICTED: cysteine protease ATG4-like isoform X1 [Cucumis sativus])

HSP 1 Score: 213.8 bits (543), Expect = 1.9e-52
Identity = 107/124 (86.29%), Postives = 112/124 (90.32%), Query Frame = 1


Query: 12  YVEDQEVDGVVVVNIDKDDLEADTSSYHCNVIRHIPLESIDPSLAIGFYCRDKDDFDNFC 71
           Y++  EV    VVNIDKDDLEADTSSYHCNVIRHIPLESIDPSLAIGFYCRDKDDFDNFC
Sbjct: 362 YLDPHEVQQ--VVNIDKDDLEADTSSYHCNVIRHIPLESIDPSLAIGFYCRDKDDFDNFC 421

Query: 72  YRASKLADESDGAPLFTVAETHSTNSGRHGSALNDCSRLVEDT-DGVVHMPNEEEAHEDD 131
           +RASKLA+ESDGAPLFTVAETHSTN GR  SALND SRLVED  DGVVHMPNEEE+HEDD
Sbjct: 422 HRASKLAEESDGAPLFTVAETHSTNPGRQSSALNDHSRLVEDDGDGVVHMPNEEESHEDD 481

Query: 132 WQFL 135
           WQFL
Sbjct: 482 WQFL 483

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BLAST of Lsi09G010720 vs. NCBI nr
Match: gi|590714446|ref|XP_007049916.1| (Peptidase family C54 protein isoform 2 [Theobroma cacao])

HSP 1 Score: 150.6 bits (379), Expect = 2.0e-33
Identity = 76/126 (60.32%), Postives = 96/126 (76.19%), Query Frame = 1


Query: 10  ILYVEDQEVDGVVVVNIDKDDLEADTSSYHCNVIRHIPLESIDPSLAIGFYCRDKDDFDN 69
           + Y++  +V   +VVN+ +D+ EADTSSYHC++IRHIPL+SIDPSLAIGF+CRDKDDFD+
Sbjct: 273 VFYLDPHDVQ--LVVNLSQDNQEADTSSYHCDIIRHIPLDSIDPSLAIGFFCRDKDDFDD 332

Query: 70  FCYRASKLADESDGAPLFTVAETHST-NSGRHGSALNDCSRLVEDTDGVVHMPNEEEAHE 129
           FC RASKLADES+GAPLFTVA+THS+     HG+AL+D   + ED    V    +   HE
Sbjct: 333 FCLRASKLADESNGAPLFTVAQTHSSFKPISHGNALDDTGEVREDDSLGVVPDMDGSIHE 392

Query: 130 DDWQFL 135
           DDWQ L
Sbjct: 393 DDWQLL 396

The following BLAST results are available for this feature:

BLAST of Lsi09G010720 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ATG4_MEDTR	2.2e-29	54.20	Cysteine protease ATG4 OS=Medicago truncatula GN=ATG4 PE=3 SV=1	[more]
ATG4A_ARATH	5.0e-29	54.03	Cysteine protease ATG4a OS=Arabidopsis thaliana GN=ATG4A PE=2 SV=1	[more]
ATG4B_ARATH	1.0e-26	48.78	Cysteine protease ATG4b OS=Arabidopsis thaliana GN=ATG4B PE=1 SV=1	[more]
ATG4A_ORYSI	8.2e-24	46.40	Cysteine protease ATG4A OS=Oryza sativa subsp. indica GN=ATG4A PE=3 SV=1	[more]
ATG4A_ORYSJ	4.1e-23	45.97	Cysteine protease ATG4A OS=Oryza sativa subsp. japonica GN=ATG4A PE=2 SV=1	[more]

BLAST of Lsi09G010720 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LK81_CUCSA	1.3e-52	86.29	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G128640 PE=3 SV=1	[more]
A0A061DVN9_THECC	1.4e-33	60.32	Peptidase family C54 protein isoform 2 OS=Theobroma cacao GN=TCM_003165 PE=3 SV=...	[more]
A0A061DMN0_THECC	1.4e-33	60.32	Peptidase family C54 protein isoform 3 OS=Theobroma cacao GN=TCM_003165 PE=3 SV=...	[more]
M5XJB4_PRUPE	4.4e-32	59.84	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004885mg PE=3 SV=1	[more]
A0A0D2SXT5_GOSRA	7.5e-32	55.88	Uncharacterized protein OS=Gossypium raimondii GN=B456_008G001300 PE=3 SV=1	[more]

BLAST of Lsi09G010720 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G44140.1	2.8e-30	54.03	Peptidase family C54 protein	[more]
AT3G59950.1	5.8e-28	48.78	Peptidase family C54 protein	[more]

BLAST of Lsi09G010720 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659082126\|ref\|XP_008441684.1\|	1.3e-53	87.90	PREDICTED: cysteine protease ATG4-like isoform X1 [Cucumis melo]	[more]
gi\|659082128\|ref\|XP_008441686.1\|	1.3e-53	87.90	PREDICTED: cysteine protease ATG4-like isoform X2 [Cucumis melo]	[more]
gi\|449442361\|ref\|XP_004138950.1\|	1.9e-52	86.29	PREDICTED: cysteine protease ATG4-like isoform X2 [Cucumis sativus]	[more]
gi\|778668355\|ref\|XP_011649086.1\|	1.9e-52	86.29	PREDICTED: cysteine protease ATG4-like isoform X1 [Cucumis sativus]	[more]
gi\|590714446\|ref\|XP_007049916.1\|	2.0e-33	60.32	Peptidase family C54 protein isoform 2 [Theobroma cacao]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR005078	Peptidase_C54

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000045	autophagosome assembly
biological_process	GO:0006501	C-terminal protein lipidation
biological_process	GO:0000422	mitophagy
biological_process	GO:0044804	nucleophagy
biological_process	GO:0051697	protein delipidation
biological_process	GO:0016485	protein processing
biological_process	GO:0006612	protein targeting to membrane
biological_process	GO:0006914	autophagy
biological_process	GO:0015031	protein transport
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004197	cysteine-type endopeptidase activity
molecular_function	GO:0008234	cysteine-type peptidase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi09G010720.1	Lsi09G010720.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005078	Peptidase C54	PANTHER	PTHR22624	APG4 AUTOPHAGY 4-RELATED	coord: 23..134 score: 2.7
IPR005078	Peptidase C54	PFAM	PF03416	Peptidase_C54	coord: 27..72 score: 2.1
None	No IPR available	PANTHER	PTHR22624:SF34	AUTOPHAGY-SPECIFIC GENE 4, ISOFORM A	coord: 23..134 score: 2.7
None	No IPR available	unknown	SSF54001	Cysteine proteinases	coord: 27..93 score: 1.08

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi09G010720	ClCG06G009970	Watermelon (Charleston Gray)	lsiwcgB031
Lsi09G010720	Cp4.1LG13g00190	Cucurbita pepo (Zucchini)	cpelsiB137

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi09G010720	Wax gourd	lsiwgoB007
Lsi09G010720	Wax gourd	lsiwgoB035
Lsi09G010720	Cucumber (Gy14) v1	cgylsiB430
Lsi09G010720	Cucurbita maxima (Rimu)	cmalsiB264
Lsi09G010720	Cucurbita maxima (Rimu)	cmalsiB265
Lsi09G010720	Cucurbita maxima (Rimu)	cmalsiB628
Lsi09G010720	Cucurbita moschata (Rifu)	cmolsiB251
Lsi09G010720	Cucurbita moschata (Rifu)	cmolsiB252
Lsi09G010720	Cucurbita moschata (Rifu)	cmolsiB616
Lsi09G010720	Cucurbita pepo (Zucchini)	cpelsiB132
Lsi09G010720	Cucurbita pepo (Zucchini)	cpelsiB136
Lsi09G010720	Cucurbita pepo (Zucchini)	cpelsiB316
Lsi09G010720	Wild cucumber (PI 183967)	cpilsiB090
Lsi09G010720	Cucumber (Chinese Long) v2	culsiB091
Lsi09G010720	Melon (DHL92) v3.5.1	lsimeB023
Lsi09G010720	Melon (DHL92) v3.5.1	lsimeB028
Lsi09G010720	Watermelon (Charleston Gray)	lsiwcgB029
Lsi09G010720	Watermelon (97103) v1	lsiwmB040
Lsi09G010720	Cucumber (Gy14) v2	cgyblsiB084
Lsi09G010720	Melon (DHL92) v3.6.1	lsimedB025
Lsi09G010720	Melon (DHL92) v3.6.1	lsimedB030
Lsi09G010720	Silver-seed gourd	carlsiB479
Lsi09G010720	Silver-seed gourd	carlsiB677
Lsi09G010720	Silver-seed gourd	carlsiB755
Lsi09G010720	Cucumber (Chinese Long) v3	cuclsiB102
Lsi09G010720	Watermelon (97103) v2	lsiwmbB026