Lsi09G008820 (gene) Bottle gourd (USVL1VR-Ls)

BLAST of Lsi09G008820 vs. Swiss-Prot
Match: AAE5_ARATH (Probable acyl-activating enzyme 5, peroxisomal OS=Arabidopsis thaliana GN=AAE5 PE=2 SV=1)

HSP 1 Score: 761.9 bits (1966), Expect = 4.8e-219
Identity = 373/558 (66.85%), Postives = 450/558 (80.65%), Query Frame = 1

Query: 1   MEDLKPSPANSSPLTPIGFIERAAVIYGDSPSIIYNSTT-YTWSETYRRCLRLASSLSSI 60
           ME +KP  ANS PLTPIGF+ERAA +YGD  SI+Y S T YTW ET  RCLR+ASSLSSI
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDARTIFVLLRHSNSKLIFVDQ 120
           GI R  VVSV++PNTPAMYEL FAVPM GAILN IN RLDART+ VLLRH  SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 121 ASCALIFEALALFPLETKRPLLVLIVDDTAMQDSPLPSFVSIHKDFVDSYEDMVQRGDSN 180
            S  L  EA+++  + T  P+LV I D    ++    + V+    F  +Y+D++ RGD +
Sbjct: 121 FSVDLAVEAISM--MTTDPPILVFIADK---EEEGGDADVADRTKFSYTYDDLIHRGDLD 180

Query: 181 FQWIRPTSEWDPIVLNYTSGTTSLPKGVVHCHRGIFTVTLDSLLEWGVPKQSVYLWTLPM 240
           F+WIRP SEWDP+VLNYTSGTTS PKGVVHCHRGIF +++DSL++W VPK  VYLWTLP+
Sbjct: 181 FKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPI 240

Query: 241 FHANGWSFPWGIAAVGGTNVCIRKFDATLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRP 300
           FHANGWS+PWGIAAVGGTNVC+RKFDA LI+ LIR H VTH+CGAPVVLNML+ + + +P
Sbjct: 241 FHANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQP 300

Query: 301 LDRPVKILTAGAPPHATVLLRTESLGFVVTHGYGLTESGGTIICCSCKSEWNRLTATEQA 360
           L+RPV ILTAGAPP A VLLR ES+GFV++HGYGLTE+ G  + C+ K +WNRL A+++A
Sbjct: 301 LNRPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRA 360

Query: 361 RLKARQGVRMLAVEAADVLDPNTRKSVKRDGASIGEIVVRGGSIMLGYFKDPEATSKAMT 420
           RLKARQGVR +     DV+DP + +SV+R+G ++GEIV+RG SIMLGY KDP  T KA+ 
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKALK 420

Query: 421 NDGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYMNPVVNEVAVVARP 480
           N GWF+TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE++LY NP VNEVAVVARP
Sbjct: 421 N-GWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARP 480

Query: 481 DEFWGETPCAFVSLKKGLTRKPTEEEIIEYCKGKLPRFMVPKTVVFTTELPKTSTGKIKK 540
           D FWGETPCAFVSLK GLT++PTE E+IEYC+ K+P++MVPKTV F  ELPKTSTGK+ K
Sbjct: 481 DVFWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMK 540

Query: 541 FVLREMAKSMGPTPKSRL 558
           FVLRE+AK MG T  SR+
Sbjct: 541 FVLREIAKKMGTTRLSRM 552

BLAST of Lsi09G008820 vs. Swiss-Prot
Match: AAE6_ARATH (Probable acyl-activating enzyme 6 OS=Arabidopsis thaliana GN=AAE6 PE=2 SV=1)

HSP 1 Score: 750.4 bits (1936), Expect = 1.4e-215
Identity = 363/551 (65.88%), Postives = 446/551 (80.94%), Query Frame = 1

Query: 1   MEDLKPSPANSSPLTPIGFIERAAVIYGDSPSIIYNSTT-YTWSETYRRCLRLASSLSSI 60
           ME++KP  ANS PLTPIGF+ERAA +YGD  SI+Y S T YTW ET  RCLR+ASSLSSI
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDARTIFVLLRHSNSKLIFVDQ 120
           GI R  VVSV++PNTPAMYEL FAVPM GAILN IN RLDART+ VLLRH  SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 121 ASCALIFEALALFPLETKRPLLVLIVDDTAMQDSPLPSFVSIHKDFVDSYEDMVQRGDSN 180
            S  L  EA+++  + T  P+LV+I D    ++  +     + K F  +Y+D+++RGD  
Sbjct: 121 FSVDLAVEAVSM--MTTDPPILVVIADKE--EEGGVADVADLSK-FSYTYDDLIERGDPG 180

Query: 181 FQWIRPTSEWDPIVLNYTSGTTSLPKGVVHCHRGIFTVTLDSLLEWGVPKQSVYLWTLPM 240
           F+WIRP SEWDP+VLNYTSGTTS PKGVVHCHRGIF +++DSL++W VPK  VYLWTLP+
Sbjct: 181 FKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPI 240

Query: 241 FHANGWSFPWGIAAVGGTNVCIRKFDATLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRP 300
           FH+NGW+ PWGIAAVGGTNVC+RKFDA LI+ LIR H VTH+CGAPVVLNML+ + +++P
Sbjct: 241 FHSNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQP 300

Query: 301 LDRPVKILTAGAPPHATVLLRTESLGFVVTHGYGLTESGGTIICCSCKSEWNRLTATEQA 360
           L+ PV ILTAG+PP ATVLLR ES+GFV++HGYGLTE+ G I+ C+ K +WN L A+++A
Sbjct: 301 LNHPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRA 360

Query: 361 RLKARQGVRMLAVEAADVLDPNTRKSVKRDGASIGEIVVRGGSIMLGYFKDPEATSKAMT 420
           RLKARQGVR +     DV+DP +  SV+R+G ++GEIV+RG S+MLGY KDP  T KA+ 
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKALK 420

Query: 421 NDGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYMNPVVNEVAVVARP 480
           N GWF+TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE++LY  P VNEVAVVARP
Sbjct: 421 N-GWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARP 480

Query: 481 DEFWGETPCAFVSLKKGLTRKPTEEEIIEYCKGKLPRFMVPKTVVFTTELPKTSTGKIKK 540
           DEFWGETPCAFVSLK G + KPTEEE++EYC+ K+P++MVPKTV F  ELPK+STGK+ K
Sbjct: 481 DEFWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTK 540

Query: 541 FVLREMAKSMG 551
           FVLR++AK MG
Sbjct: 541 FVLRDIAKKMG 545

BLAST of Lsi09G008820 vs. Swiss-Prot
Match: AAE8_ARATH (Probable acyl-activating enzyme 8 OS=Arabidopsis thaliana GN=AAE8 PE=2 SV=1)

HSP 1 Score: 713.8 bits (1841), Expect = 1.5e-204
Identity = 357/551 (64.79%), Postives = 429/551 (77.86%), Query Frame = 1

Query: 1   MEDLKPSPANSSPLTPIGFIERAAVIYGDSPSIIY-NSTTYTWSETYRRCLRLASSLSSI 60
           MEDLKPS ANS PLT +GF+ERAA +YGD  SI+Y NST YTW ET  RCL +AS+LSSI
Sbjct: 1   MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60

Query: 61  GIQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDARTIFVLLRHSNSKLIFVDQ 120
           GI R  VVSV++ NTP MYEL F+VPM GAILN IN RLDART+ VLLRH  SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120

Query: 121 ASCALIFEALALFPLETKRPLLVLIVDDTAMQDSPLPSFVSIHKDFVDSYEDMVQRGDSN 180
               L  EA+ +       P+LVLI ++   +     + V+    F   Y D++ RG+ +
Sbjct: 121 FYSDLAVEAITML---LNPPILVLIANEEEEEGG---AEVTERSKFCYLYSDLITRGNPD 180

Query: 181 FQWIRPTSEWDPIVLNYTSGTTSLPKGVVHCHRGIFTVTLDSLLEWGVPKQSVYLWTLPM 240
           F+WIRP SEWDPIV+NYTSGTTS PKGVVHCHRGIF +TLDSL +W VPK  VYLWTLP+
Sbjct: 181 FKWIRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPI 240

Query: 241 FHANGWSFPWGIAAVGGTNVCIRKFDATLIFSLIRRHHVTHLCGAPVVLNMLTNSPDN-R 300
           FHANGW++PWGIAAVGGTNVC+RK  A  I+ LIR H VTH+ GAP+VL +L+ S ++ +
Sbjct: 241 FHANGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQ 300

Query: 301 PLDRPVKILTAGAPPHATVLLRTESLGFVVTHGYGLTESGGTIICCSCKSEWNRLTATEQ 360
           PL  PV  LTAG+ P ATVLLR ESLGF+V+HGYGLTE+ G I+ C+ K  WNRL A++Q
Sbjct: 301 PLKSPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQ 360

Query: 361 ARLKARQGVRMLAVEAADVLDPNTRKSVKRDGASIGEIVVRGGSIMLGYFKDPEATSKAM 420
           A+LK+RQGVR +     DV+DP + +SV+RDG ++GEIV+RG SIMLGY K+P  T  + 
Sbjct: 361 AQLKSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNSF 420

Query: 421 TNDGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYMNPVVNEVAVVAR 480
            N GWFFTGD+GV+H DGYLEIKDRSKDVIISGGEN+SSVEVE++LY NP VNE AVVAR
Sbjct: 421 KN-GWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVAR 480

Query: 481 PDEFWGETPCAFVSLKKGLTRKPTEEEIIEYCKGKLPRFMVPKTVVFTTELPKTSTGKIK 540
           PDEFWGETPCAFVSLK GLTRKPT++EIIEYCK K+PR+M PKTV F  ELPKTSTGKI 
Sbjct: 481 PDEFWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKII 540

Query: 541 KFVLREMAKSM 550
           K +L+E+AK+M
Sbjct: 541 KSLLKEIAKNM 544

BLAST of Lsi09G008820 vs. Swiss-Prot
Match: AAE4_ARATH (Probable acyl-activating enzyme 4 OS=Arabidopsis thaliana GN=AEE4 PE=2 SV=1)

HSP 1 Score: 699.9 bits (1805), Expect = 2.2e-200
Identity = 332/551 (60.25%), Postives = 424/551 (76.95%), Query Frame = 1

Query: 1   MEDLKPSPANSSPLTPIGFIERAAVIYGDSPSIIYNSTTYTWSETYRRCLRLASSLSS-- 60
           ME L P  +NS PLT +GF+ERAA ++GDSPS+++ +T +TWSET+ RCLR+AS+LSS  
Sbjct: 1   MELLLPHASNSCPLTVLGFLERAASVFGDSPSLLHTTTVHTWSETHSRCLRIASTLSSAS 60

Query: 61  IGIQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDARTIFVLLRHSNSKLIFVD 120
           +GI RG VVSVI PN P++YEL FAVPM GA+LN IN RLDA  + VLLRHS SKL+FVD
Sbjct: 61  LGINRGQVVSVIGPNVPSVYELQFAVPMSGAVLNNINPRLDAHALSVLLRHSESKLVFVD 120

Query: 121 QASCALIFEALALFPLETKRPLLVLIVDDTAMQDSPLPSFVSIHKDFVDSYEDMVQRGDS 180
             S +L+ EA++  P + +RP LV++ D   M     PS  S   DF+D+YE  ++RGD 
Sbjct: 121 HHSSSLVLEAVSFLP-KDERPRLVILNDGNDM-----PSSSSADMDFLDTYEGFMERGDL 180

Query: 181 NFQWIRPTSEWDPIVLNYTSGTTSLPKGVVHCHRGIFTVTLDSLLEWGVPKQSVYLWTLP 240
            F+W+RP SEW P+VLNYTSGTTS PKGVVH HR +F  T++SLL+W +P + VYLWTLP
Sbjct: 181 RFKWVRPKSEWTPMVLNYTSGTTSSPKGVVHSHRSVFMSTINSLLDWSLPNRPVYLWTLP 240

Query: 241 MFHANGWSFPWGIAAVGGTNVCIRKFDATLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNR 300
           MFHANGWS+ W  AAVG  N+C+ + D   IF+LI ++ VTH+C AP+VLNMLTN P  +
Sbjct: 241 MFHANGWSYTWATAAVGARNICVTRVDVPTIFNLIDKYQVTHMCAAPMVLNMLTNHPAQK 300

Query: 301 PLDRPVKILTAGAPPHATVLLRTESLGFVVTHGYGLTESGGTIICCSCKSEWNRLTATEQ 360
           PL  PVK++TAGAPP ATV+ + E+LGF V+HGYG+TE+GG ++ C+ K EW+RL   E+
Sbjct: 301 PLQSPVKVMTAGAPPPATVISKAEALGFDVSHGYGMTETGGLVVSCALKPEWDRLEPDER 360

Query: 361 ARLKARQGVRMLAVEAADVLDPNTRKSVKRDGASIGEIVVRGGSIMLGYFKDPEATSKAM 420
           A+ K+RQG+R       DV DP + KSVK DGA++GEIV RGGS+MLGY+KDPE T+ +M
Sbjct: 361 AKQKSRQGIRTAVFAEVDVRDPISGKSVKHDGATVGEIVFRGGSVMLGYYKDPEGTAASM 420

Query: 421 TNDGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYMNPVVNEVAVVAR 480
             DGWF+TGD+GVMHPDGYLE+KDRSKDV+I GGEN+SS E+E++LY NP + E AVVA+
Sbjct: 421 REDGWFYTGDIGVMHPDGYLEVKDRSKDVVICGGENISSTELEAVLYTNPAIKEAAVVAK 480

Query: 481 PDEFWGETPCAFVSLKKGLTRKPTEEEIIEYCKGKLPRFMVPKTVVFTTELPKTSTGKIK 540
           PD+ WGETPCAFVSLK       TE EI E+CK KLP++MVP+ VVF  ELPKTSTGKI+
Sbjct: 481 PDKMWGETPCAFVSLKYH-DGSVTEREIREFCKTKLPKYMVPRNVVFLEELPKTSTGKIQ 540

Query: 541 KFVLREMAKSM 550
           KF+LR+MAKS+
Sbjct: 541 KFLLRQMAKSL 544

BLAST of Lsi09G008820 vs. Swiss-Prot
Match: AEE9_ARATH (Probable acyl-activating enzyme 9 OS=Arabidopsis thaliana GN=AEE9 PE=2 SV=1)

HSP 1 Score: 696.4 bits (1796), Expect = 2.5e-199
Identity = 340/558 (60.93%), Postives = 431/558 (77.24%), Query Frame = 1

Query: 1   MEDLKPSPANSSPLTPIGFIERAAVIYGDSPSIIYNSTT-YTWSETYRRCLRLASSL--S 60
           ME L P P+NS+PLT +GF++RAA +YGD PSI++ + T +TWSET+ RCLR+AS+L  S
Sbjct: 1   MELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTTNTVHTWSETHNRCLRIASALTSS 60

Query: 61  SIGIQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDARTIFVLLRHSNSKLIFV 120
           S+GI RG VVSV+ PN P++YEL FAVPM GAILN IN RLDA  + VLLRHS SKL+FV
Sbjct: 61  SLGINRGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESKLVFV 120

Query: 121 DQASCALIFEALALFPLETKRPLLVLIVDDTAMQDSPL-PSFVSIHKDFVDSYEDMVQRG 180
           D  S +++ EA++ F  + ++P LVL+ DD   +D  L PS  S   DF+D+Y+ +++RG
Sbjct: 121 DPNSISVVLEAVS-FMRQNEKPHLVLLDDD--QEDGSLSPSAAS---DFLDTYQGVMERG 180

Query: 181 DSNFQWIRPTSEWDPIVLNYTSGTTSLPKGVVHCHRGIFTVTLDSLLEWGVPKQSVYLWT 240
           DS F+WIRP +EW P++LNYTSGTTS PKGVV  HR IF +T+ SLL+W  P + VYLWT
Sbjct: 181 DSRFKWIRPQTEWQPMILNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWHFPNRPVYLWT 240

Query: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDATLIFSLIRRHHVTHLCGAPVVLNMLTNSPD 300
           LPMFHANGW + WG AAVG TNVC R+ DA  I+ LI +HHVTH+C AP+VLNMLTN P 
Sbjct: 241 LPMFHANGWGYTWGTAAVGATNVCTRRVDAPTIYDLIDKHHVTHMCAAPMVLNMLTNYPS 300

Query: 301 NRPLDRPVKILTAGAPPHATVLLRTESLGFVVTHGYGLTESGGTIICCSCKSEWNRLTAT 360
            +PL  PV+++TAGAPP A ++ R E+LGF V HGYGLTE+GG ++ C+ K+EW+ L   
Sbjct: 301 RKPLKNPVQVMTAGAPPPAAIISRAETLGFNVGHGYGLTETGGPVVSCAWKAEWDHLDPL 360

Query: 361 EQARLKARQGVRMLAVEAADVLDPNTRKSVKRDGASIGEIVVRGGSIMLGYFKDPEATSK 420
           E+ARLK+RQGVR +     DV DP T KSV+ DG S+GEIV++GGS+MLGY+KDPE T+ 
Sbjct: 361 ERARLKSRQGVRTIGFAEVDVRDPRTGKSVEHDGVSVGEIVLKGGSVMLGYYKDPEGTAA 420

Query: 421 AMTNDGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYMNPVVNEVAVV 480
            M  DGWF++GDVGV+H DGYLE+KDRSKDVII GGEN+SS EVE++LY NPVV E AVV
Sbjct: 421 CMREDGWFYSGDVGVIHEDGYLEVKDRSKDVIICGGENISSAEVETVLYTNPVVKEAAVV 480

Query: 481 ARPDEFWGETPCAFVSLK-----KGLTRKPTEEEIIEYCKGKLPRFMVPKTVVFTTELPK 540
           A+PD+ WGETPCAFVSLK      GL    TE EI E+CK +LP++MVP+ V+F  ELPK
Sbjct: 481 AKPDKMWGETPCAFVSLKYDSNGNGLV---TEREIREFCKTRLPKYMVPRKVIFQEELPK 540

Query: 541 TSTGKIKKFVLREMAKSM 550
           TSTGKI+KF+LR+MAKS+
Sbjct: 541 TSTGKIQKFLLRQMAKSL 549

BLAST of Lsi09G008820 vs. TrEMBL
Match: A0A0A0KMT3_CUCSA (AMP dependent ligase OS=Cucumis sativus GN=Csa_5G318390 PE=4 SV=1)

HSP 1 Score: 988.8 bits (2555), Expect = 2.7e-285
Identity = 483/557 (86.71%), Postives = 514/557 (92.28%), Query Frame = 1

Query: 1   MEDLKPSPANSSPLTPIGFIERAAVIYGDSPSIIYNSTTYTWSETYRRCLRLASSLSSIG 60
           MEDLKP PANS PLTPIGFIERAA+IYGDS SIIYNSTTYTWSET+RRCL+LASS+SS G
Sbjct: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDARTIFVLLRHSNSKLIFVDQA 120
           IQRGHVVSV APNTPAMYELHFAVPM GAILNTINLRLD RTI +LLRHS SKLIFVDQ 
Sbjct: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120

Query: 121 SCALIFEALALFPLETKRPLLVLIVDDTAMQDSPLPSFVSIHKDFVDSYEDMVQRGDSNF 180
           SCALIF+ALALFP + KRPLLVLI DDTA QDS LPS VSI  +FVDSYE+MV+ GDS F
Sbjct: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180

Query: 181 QWIRPTSEWDPIVLNYTSGTTSLPKGVVHCHRGIFTVTLDSLLEWGVPKQSVYLWTLPMF 240
           QWIRP SEWDPIVLNYTSGTTS PKGVV+CHRG+FTV LDS+LEWGVPKQSVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240

Query: 241 HANGWSFPWGIAAVGGTNVCIRKFDATLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRPL 300
           H NGWSFPWGIAAVGGTNVCIRKFD+ LIFSLIRRHHVTHLCGAPVVLNMLTNSPDN PL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 301 DRPVKILTAGAPPHATVLLRTESLGFVVTHGYGLTESGGTIICCSCKSEWNRLTATEQAR 360
           DRPVKILTAGAPP A+VLLRTESLGFVVTHGYGLTES GTIICCS KSEWN+L A EQAR
Sbjct: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 361 LKARQGVRMLAVEAADVLDPNTRKSVKRDGASIGEIVVRGGSIMLGYFKDPEATSKAMTN 420
           LKARQGVRMLAVEA DVLDP TRKSVKRDGASIGEIVVRG S+MLGY KD EATSKAM+ 
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420

Query: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYMNPVVNEVAVVARPD 480
           DGWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVES+LYMNP+VNE AVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 481 EFWGETPCAFVSLKKGLTRKPTEEEIIEYCKGKLPRFMVPKTVVFTTELPKTSTGKIKKF 540
           EFWGETPCAF+SLK+GL+RKPTEEEIIEYCKGKLP+FMVPKTV+F TE+PKT+TGK +KF
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540

Query: 541 VLREMAKSMGPTPKSRL 558
           VLR+ AKS+GP  KSRL
Sbjct: 541 VLRQKAKSLGPPQKSRL 557

BLAST of Lsi09G008820 vs. TrEMBL
Match: A0A0A0KT33_CUCSA (AMP dependent ligase OS=Cucumis sativus GN=Csa_5G317890 PE=4 SV=1)

HSP 1 Score: 919.1 bits (2374), Expect = 2.6e-264
Identity = 456/557 (81.87%), Postives = 496/557 (89.05%), Query Frame = 1

Query: 1   MEDLKPSPANSSPLTPIGFIERAAVIYGDSPSIIYNSTTYTWSETYRRCLRLASSLSSIG 60
           ME LKP+PANSSPLTPIGF+ERA++IY DSPSIIY+STT+TWS+TYRRCLRLASSLSSIG
Sbjct: 22  MEHLKPTPANSSPLTPIGFLERASLIYADSPSIIYSSTTFTWSQTYRRCLRLASSLSSIG 81

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDARTIFVLLRHSNSKLIFVDQA 120
           I +GHVVSVIAPNTP MYEL FAVPM GAIL+ INLRLDARTI V LRHS SKL+FVDQA
Sbjct: 82  IHKGHVVSVIAPNTPPMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 141

Query: 121 SCALIFEALALFPLETKRPLLVLIVDDTAMQDSPLPSFVSIHKDFVDSYEDMVQRGDSNF 180
           S  LI +ALALFP E KRPLLVLI DD A++DS   S V+I  D VD YEDMVQ+GD  F
Sbjct: 142 SSGLISDALALFPPEAKRPLLVLISDDAAVEDSA--SSVAIGGDVVDLYEDMVQKGDPEF 201

Query: 181 QWIRPTSEWDPIVLNYTSGTTSLPKGVVHCHRGIFTVTLDSLLEWGVPKQSVYLWTLPMF 240
           +WIRP SEWDPIV+NYTSGTTS PKGVVH HRGIFTVTLDSL+EWGVPK SVYLWTLPMF
Sbjct: 202 RWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMF 261

Query: 241 HANGWSFPWGIAAVGGTNVCIRKFDATLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRPL 300
           HANGWSFP+ IAAVGGTN+CIRKFDA LIFSLI RHHVTH+CGAPVVLNMLTNSP+NRPL
Sbjct: 262 HANGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHMCGAPVVLNMLTNSPNNRPL 321

Query: 301 DRPVKILTAGAPPHATVLLRTESLGFVVTHGYGLTESGGTIICCSCKSEWNRLTATEQAR 360
           +RPVKILTAGAPP A VLLRTESLGF V+HGYGLTE+GG +I CS K EWN+L ATE+AR
Sbjct: 322 ERPVKILTAGAPPPAAVLLRTESLGFDVSHGYGLTETGGLVIFCSWKLEWNQLPATERAR 381

Query: 361 LKARQGVRMLAVEAADVLDPNTRKSVKRDGASIGEIVVRGGSIMLGYFKDPEATSKAMTN 420
           LKARQGVR LA+  ADVLD  T K V+RDG SIGEIV+RGGS+MLGYFKDPEAT KAMT 
Sbjct: 382 LKARQGVRTLAMAEADVLDSGTGKRVQRDGVSIGEIVIRGGSMMLGYFKDPEATKKAMTE 441

Query: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYMNPVVNEVAVVARPD 480
           +GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVES+LY NP VNE AVVARPD
Sbjct: 442 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 501

Query: 481 EFWGETPCAFVSLKKGLTRKPTEEEIIEYCKGKLPRFMVPKTVVFTTELPKTSTGKIKKF 540
           EFWGETPCAFVSL++GLTR PTEEEIIEYCKGKL  FMVPKTVVF  +LPKTSTGKI+KF
Sbjct: 502 EFWGETPCAFVSLREGLTRVPTEEEIIEYCKGKLAGFMVPKTVVFMEQLPKTSTGKIQKF 561

Query: 541 VLREMAKSMGPTPKSRL 558
           +LREMAKSMG +PKSRL
Sbjct: 562 LLREMAKSMGCSPKSRL 576

BLAST of Lsi09G008820 vs. TrEMBL
Match: A0A068U0B9_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00036869001 PE=4 SV=1)

HSP 1 Score: 824.7 bits (2129), Expect = 6.7e-236
Identity = 386/557 (69.30%), Postives = 472/557 (84.74%), Query Frame = 1

Query: 1   MEDLKPSPANSSPLTPIGFIERAAVIYGDSPSIIYNSTTYTWSETYRRCLRLASSLSSIG 60
           M++LKP PANS+PLTP+GF+ERAA +YGD PS++YN+ TYTWS+T+ RCLR+ASS+ S+G
Sbjct: 1   MDELKPGPANSAPLTPLGFLERAATVYGDCPSVVYNNATYTWSDTHVRCLRVASSIVSLG 60

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDARTIFVLLRHSNSKLIFVDQA 120
           I+RG VVSV+APN PAMYE HFAVPM GA+L+TINLRLDART+ +LL HS SKLI VD  
Sbjct: 61  IKRGQVVSVVAPNIPAMYEAHFAVPMAGAVLSTINLRLDARTVSILLHHSESKLILVDYQ 120

Query: 121 SCALIFEALALFPLETKRPLLVLIVDDTAMQDSPLPSFVSIHKDFVDSYEDMVQRGDSNF 180
           S +L+ EAL+LFP  ++RPLLVLI DD +    P     S   DF  +YE +VQ+GD  F
Sbjct: 121 STSLVLEALSLFPPNSQRPLLVLITDDASPSIPP-----SNFPDFYCTYESLVQKGDPGF 180

Query: 181 QWIRPTSEWDPIVLNYTSGTTSLPKGVVHCHRGIFTVTLDSLLEWGVPKQSVYLWTLPMF 240
           +W+RP S++DPI+LNYTSGTTS PKGVVHCHRGIF VT+DSL+EW VPK+ VYLWTLPMF
Sbjct: 181 KWVRPKSDFDPIILNYTSGTTSSPKGVVHCHRGIFIVTVDSLIEWSVPKEPVYLWTLPMF 240

Query: 241 HANGWSFPWGIAAVGGTNVCIRKFDATLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRPL 300
           HANGWS+PWG+AAVGGTN+C+RKFDA +++S IR+H+VTHLCGAPVVLNM++N P   PL
Sbjct: 241 HANGWSYPWGMAAVGGTNICLRKFDAAVVYSAIRQHNVTHLCGAPVVLNMISNFPMGEPL 300

Query: 301 DRPVKILTAGAPPHATVLLRTESLGFVVTHGYGLTESGGTIICCSCKSEWNRLTATEQAR 360
             PV+I+TAGAPP A VLLRTE+LGF V+HGYGLTE+GG ++ C+ K++WNRL ATE+AR
Sbjct: 301 KNPVEIMTAGAPPPAAVLLRTEALGFKVSHGYGLTETGGLVVSCTWKNKWNRLPATERAR 360

Query: 361 LKARQGVRMLAVEAADVLDPNTRKSVKRDGASIGEIVVRGGSIMLGYFKDPEATSKAMTN 420
           LKARQGVR L +   DV++P T  SVKRDG+++GE+V+RGG IMLGY KDPE TSK+   
Sbjct: 361 LKARQGVRTLGMAEIDVIEPETGVSVKRDGSTLGELVLRGGCIMLGYLKDPEGTSKSTRE 420

Query: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYMNPVVNEVAVVARPD 480
           +GWF+TGDV VMHPDGYLEIKDRSKDVIISGGENLSSVEVES+LYM+P VNE AVVARPD
Sbjct: 421 NGWFYTGDVAVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMHPAVNEAAVVARPD 480

Query: 481 EFWGETPCAFVSLKKGLTRKPTEEEIIEYCKGKLPRFMVPKTVVFTTELPKTSTGKIKKF 540
           EFWGETPCAFVSLK  + ++P E+++IE+C+GKLP++MVPKTVVF  ELPKTSTGKI+KF
Sbjct: 481 EFWGETPCAFVSLKADVKQRPAEKDVIEFCRGKLPKYMVPKTVVFRQELPKTSTGKIQKF 540

Query: 541 VLREMAKSMGPTPKSRL 558
           VLREMAK++G +  SRL
Sbjct: 541 VLREMAKAIGSSKVSRL 552

BLAST of Lsi09G008820 vs. TrEMBL
Match: A0A061ESR0_THECC (Acyl activating enzyme 5 OS=Theobroma cacao GN=TCM_020359 PE=4 SV=1)

HSP 1 Score: 818.1 bits (2112), Expect = 6.3e-234
Identity = 389/550 (70.73%), Postives = 466/550 (84.73%), Query Frame = 1

Query: 1   MEDLKPSPANSSPLTPIGFIERAAVIYGDSPSIIYNSTTYTWSETYRRCLRLASSLSSIG 60
           ME+LKP PA+SSPLTP+GF+ERAA +YGD  SIIYN+T+YTWS+T+RRCL++ASSLSS G
Sbjct: 51  MEELKPRPASSSPLTPLGFLERAATVYGDCTSIIYNNTSYTWSDTHRRCLQIASSLSSTG 110

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDARTIFVLLRHSNSKLIFVDQA 120
           I+RGHVVSV+APN PAMYELHFAVPM GA+LN+IN RLDAR + +LLRH  SKL+FVD  
Sbjct: 111 IKRGHVVSVLAPNIPAMYELHFAVPMTGAVLNSINTRLDARIVSILLRHCESKLLFVDTL 170

Query: 121 SCALIFEALALFPLETKRPLLVLIVDDTAMQDSPLPSFVSIHKDFVDSYEDMVQRGDSNF 180
           S +L  EA++LFP  TK PLLVLI DD+A  +S   S  ++   F  +YE +V++GD +F
Sbjct: 171 SRSLALEAISLFPPNTKPPLLVLIGDDSADAESSDSSSSTVDSRFCCTYESLVKKGDPDF 230

Query: 181 QWIRPTSEWDPIVLNYTSGTTSLPKGVVHCHRGIFTVTLDSLLEWGVPKQSVYLWTLPMF 240
           +W+RP SEW+PIVLNYTSGTTS PKGVVH HRG F +TLDSL++WGVPKQ VYLWTLPMF
Sbjct: 231 KWVRPQSEWNPIVLNYTSGTTSSPKGVVHSHRGTFMMTLDSLIDWGVPKQPVYLWTLPMF 290

Query: 241 HANGWSFPWGIAAVGGTNVCIRKFDATLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRPL 300
           HANGWSF WGIAAVGG NVC+RKFDA +I+SLI++H VTH+CGAPVVL+ML+N P+ +PL
Sbjct: 291 HANGWSFTWGIAAVGGINVCVRKFDAPIIYSLIKKHGVTHMCGAPVVLSMLSNFPEIKPL 350

Query: 301 DRPVKILTAGAPPHATVLLRTESLGFVVTHGYGLTESGGTIICCSCKSEWNRLTATEQAR 360
             PVKILTAGAPP A VL RTE+LGF+V+HGYGLTE+GG ++ C+ K  WN+L ATE+AR
Sbjct: 351 QSPVKILTAGAPPPAAVLFRTEALGFIVSHGYGLTETGGLVVSCAWKRGWNKLPATERAR 410

Query: 361 LKARQGVRMLAVEAADVLDPNTRKSVKRDGASIGEIVVRGGSIMLGYFKDPEATSKAMTN 420
           LKARQGVR L +  ADV+D  +  SVKRDG+++GEIV+RG  IMLGY KDP  T+K M  
Sbjct: 411 LKARQGVRTLGMTEADVVDTESGLSVKRDGSTLGEIVLRGACIMLGYLKDPNGTNKCMKE 470

Query: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYMNPVVNEVAVVARPD 480
           +GWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESILY +P + E AVVARPD
Sbjct: 471 NGWFFTGDVGVMHPDGYMEIKDRSKDVIISGGENLSSVEVESILYNHPAIIEAAVVARPD 530

Query: 481 EFWGETPCAFVSLKKGLTRKPTEEEIIEYCKGKLPRFMVPKTVVFTTELPKTSTGKIKKF 540
           ++WGETPCAFVSLK GLT+KP+E+EIIEYC+ KLP +MVPKTVVF  ELPKTSTGKI+KF
Sbjct: 531 DYWGETPCAFVSLKAGLTQKPSEKEIIEYCRAKLPHYMVPKTVVFKEELPKTSTGKIQKF 590

Query: 541 VLREMAKSMG 551
           +LRE+AK MG
Sbjct: 591 LLREIAKGMG 600

BLAST of Lsi09G008820 vs. TrEMBL
Match: B9S6X4_RICCO (AMP dependent ligase, putative OS=Ricinus communis GN=RCOM_1328840 PE=4 SV=1)

HSP 1 Score: 816.6 bits (2108), Expect = 1.8e-233
Identity = 387/559 (69.23%), Postives = 463/559 (82.83%), Query Frame = 1

Query: 1   MEDLKPSPANSSPLTPIGFIERAAVIYGDSPSIIYNSTTYTWSETYRRCLRLASSLSSIG 60
           M+ LKP PANS PLTP+ F+ERA+ +YGD PSI+YN+ TYTWS+T+RRCL+LASSLSS G
Sbjct: 1   MDHLKPKPANSFPLTPLSFLERASTVYGDCPSIVYNTATYTWSQTHRRCLQLASSLSSGG 60

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDARTIFVLLRHSNSKLIFVDQA 120
             RG VVSV+APNTP MYEL FAVPM GA+LN IN RLDART+ VLLRHS SKL+FVD  
Sbjct: 61  FTRGDVVSVVAPNTPPMYELQFAVPMSGAVLNNINTRLDARTVSVLLRHSQSKLVFVDCL 120

Query: 121 SCALIFEALALFPLETKRPLLVLIVDDTAMQDSPLPSFVSIHK--DFVDSYEDMVQRGDS 180
           S  L+ EA++LFP   KRP LVLI DD    ++P  S  S     DF  +YE MV++GD 
Sbjct: 121 SRDLVLEAVSLFPSSVKRPTLVLITDD---DEAPASSSTSSSSTVDFCCTYESMVEKGDP 180

Query: 181 NFQWIRPTSEWDPIVLNYTSGTTSLPKGVVHCHRGIFTVTLDSLLEWGVPKQSVYLWTLP 240
            F+W++P SEWDP++LNYTSGTTS PKGVVHCHRGI+ +T++SL++W VP+Q VYLWTLP
Sbjct: 181 EFKWVQPQSEWDPMILNYTSGTTSSPKGVVHCHRGIYIITVESLIDWNVPRQPVYLWTLP 240

Query: 241 MFHANGWSFPWGIAAVGGTNVCIRKFDATLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNR 300
           +FHANGWSFPWG+AAVGGTN+CIRKFDA+ I+ LI+RH VTH+CGAPVVLNMLTNSPDN 
Sbjct: 241 LFHANGWSFPWGMAAVGGTNICIRKFDASSIYGLIKRHGVTHMCGAPVVLNMLTNSPDNE 300

Query: 301 PLDRPVKILTAGAPPHATVLLRTESLGFVVTHGYGLTESGGTIICCSCKSEWNRLTATEQ 360
           PL RPV ILTAGAPP A VL RTESLGFVV+HGYGLTE+ G ++ C+ K +WN + A+E+
Sbjct: 301 PLKRPVHILTAGAPPPAAVLFRTESLGFVVSHGYGLTETAGLVVSCAWKPKWNTVPASER 360

Query: 361 ARLKARQGVRMLAVEAADVLDPNTRKSVKRDGASIGEIVVRGGSIMLGYFKDPEATSKAM 420
           ARLKARQGVR+      DV+DP T  SVKRDG S+GEIV+RGG +M+GY KDP+ TSK +
Sbjct: 361 ARLKARQGVRIAGFTEIDVIDPKTGLSVKRDGLSLGEIVLRGGCVMMGYLKDPDGTSKCL 420

Query: 421 TNDGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYMNPVVNEVAVVAR 480
            +DGWF+TGDVGVMHPDGYLEIKDRSKDVIISGGEN+SSVEVES+LY NPVVNE AVVAR
Sbjct: 421 KDDGWFYTGDVGVMHPDGYLEIKDRSKDVIISGGENISSVEVESVLYSNPVVNEAAVVAR 480

Query: 481 PDEFWGETPCAFVSLKKGLTRKPTEEEIIEYCKGKLPRFMVPKTVVFTTELPKTSTGKIK 540
           PDE+WGETPCAFVSL+ G   K +E++IIEYC+ K+P +MVPKTVVF  ELPKTSTGKI+
Sbjct: 481 PDEYWGETPCAFVSLRDGAREKVSEKDIIEYCREKMPHYMVPKTVVFKEELPKTSTGKIQ 540

Query: 541 KFVLREMAKSMGPTPKSRL 558
           KFVLR++AK+MG +  SR+
Sbjct: 541 KFVLRDIAKAMGASKVSRM 556

BLAST of Lsi09G008820 vs. TAIR10
Match: AT5G16370.1 (AT5G16370.1 acyl activating enzyme 5)

HSP 1 Score: 761.9 bits (1966), Expect = 2.7e-220
Identity = 373/558 (66.85%), Postives = 450/558 (80.65%), Query Frame = 1

Query: 1   MEDLKPSPANSSPLTPIGFIERAAVIYGDSPSIIYNSTT-YTWSETYRRCLRLASSLSSI 60
           ME +KP  ANS PLTPIGF+ERAA +YGD  SI+Y S T YTW ET  RCLR+ASSLSSI
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDARTIFVLLRHSNSKLIFVDQ 120
           GI R  VVSV++PNTPAMYEL FAVPM GAILN IN RLDART+ VLLRH  SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 121 ASCALIFEALALFPLETKRPLLVLIVDDTAMQDSPLPSFVSIHKDFVDSYEDMVQRGDSN 180
            S  L  EA+++  + T  P+LV I D    ++    + V+    F  +Y+D++ RGD +
Sbjct: 121 FSVDLAVEAISM--MTTDPPILVFIADK---EEEGGDADVADRTKFSYTYDDLIHRGDLD 180

Query: 181 FQWIRPTSEWDPIVLNYTSGTTSLPKGVVHCHRGIFTVTLDSLLEWGVPKQSVYLWTLPM 240
           F+WIRP SEWDP+VLNYTSGTTS PKGVVHCHRGIF +++DSL++W VPK  VYLWTLP+
Sbjct: 181 FKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPI 240

Query: 241 FHANGWSFPWGIAAVGGTNVCIRKFDATLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRP 300
           FHANGWS+PWGIAAVGGTNVC+RKFDA LI+ LIR H VTH+CGAPVVLNML+ + + +P
Sbjct: 241 FHANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQP 300

Query: 301 LDRPVKILTAGAPPHATVLLRTESLGFVVTHGYGLTESGGTIICCSCKSEWNRLTATEQA 360
           L+RPV ILTAGAPP A VLLR ES+GFV++HGYGLTE+ G  + C+ K +WNRL A+++A
Sbjct: 301 LNRPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRA 360

Query: 361 RLKARQGVRMLAVEAADVLDPNTRKSVKRDGASIGEIVVRGGSIMLGYFKDPEATSKAMT 420
           RLKARQGVR +     DV+DP + +SV+R+G ++GEIV+RG SIMLGY KDP  T KA+ 
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKALK 420

Query: 421 NDGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYMNPVVNEVAVVARP 480
           N GWF+TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE++LY NP VNEVAVVARP
Sbjct: 421 N-GWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARP 480

Query: 481 DEFWGETPCAFVSLKKGLTRKPTEEEIIEYCKGKLPRFMVPKTVVFTTELPKTSTGKIKK 540
           D FWGETPCAFVSLK GLT++PTE E+IEYC+ K+P++MVPKTV F  ELPKTSTGK+ K
Sbjct: 481 DVFWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMK 540

Query: 541 FVLREMAKSMGPTPKSRL 558
           FVLRE+AK MG T  SR+
Sbjct: 541 FVLREIAKKMGTTRLSRM 552

BLAST of Lsi09G008820 vs. TAIR10
Match: AT5G16340.1 (AT5G16340.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 750.4 bits (1936), Expect = 8.2e-217
Identity = 363/551 (65.88%), Postives = 446/551 (80.94%), Query Frame = 1

Query: 1   MEDLKPSPANSSPLTPIGFIERAAVIYGDSPSIIYNSTT-YTWSETYRRCLRLASSLSSI 60
           ME++KP  ANS PLTPIGF+ERAA +YGD  SI+Y S T YTW ET  RCLR+ASSLSSI
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDARTIFVLLRHSNSKLIFVDQ 120
           GI R  VVSV++PNTPAMYEL FAVPM GAILN IN RLDART+ VLLRH  SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 121 ASCALIFEALALFPLETKRPLLVLIVDDTAMQDSPLPSFVSIHKDFVDSYEDMVQRGDSN 180
            S  L  EA+++  + T  P+LV+I D    ++  +     + K F  +Y+D+++RGD  
Sbjct: 121 FSVDLAVEAVSM--MTTDPPILVVIADKE--EEGGVADVADLSK-FSYTYDDLIERGDPG 180

Query: 181 FQWIRPTSEWDPIVLNYTSGTTSLPKGVVHCHRGIFTVTLDSLLEWGVPKQSVYLWTLPM 240
           F+WIRP SEWDP+VLNYTSGTTS PKGVVHCHRGIF +++DSL++W VPK  VYLWTLP+
Sbjct: 181 FKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPI 240

Query: 241 FHANGWSFPWGIAAVGGTNVCIRKFDATLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRP 300
           FH+NGW+ PWGIAAVGGTNVC+RKFDA LI+ LIR H VTH+CGAPVVLNML+ + +++P
Sbjct: 241 FHSNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQP 300

Query: 301 LDRPVKILTAGAPPHATVLLRTESLGFVVTHGYGLTESGGTIICCSCKSEWNRLTATEQA 360
           L+ PV ILTAG+PP ATVLLR ES+GFV++HGYGLTE+ G I+ C+ K +WN L A+++A
Sbjct: 301 LNHPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRA 360

Query: 361 RLKARQGVRMLAVEAADVLDPNTRKSVKRDGASIGEIVVRGGSIMLGYFKDPEATSKAMT 420
           RLKARQGVR +     DV+DP +  SV+R+G ++GEIV+RG S+MLGY KDP  T KA+ 
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKALK 420

Query: 421 NDGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYMNPVVNEVAVVARP 480
           N GWF+TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE++LY  P VNEVAVVARP
Sbjct: 421 N-GWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARP 480

Query: 481 DEFWGETPCAFVSLKKGLTRKPTEEEIIEYCKGKLPRFMVPKTVVFTTELPKTSTGKIKK 540
           DEFWGETPCAFVSLK G + KPTEEE++EYC+ K+P++MVPKTV F  ELPK+STGK+ K
Sbjct: 481 DEFWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTK 540

Query: 541 FVLREMAKSMG 551
           FVLR++AK MG
Sbjct: 541 FVLRDIAKKMG 545

BLAST of Lsi09G008820 vs. TAIR10
Match: AT1G75960.1 (AT1G75960.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 713.8 bits (1841), Expect = 8.5e-206
Identity = 357/551 (64.79%), Postives = 429/551 (77.86%), Query Frame = 1

Query: 1   MEDLKPSPANSSPLTPIGFIERAAVIYGDSPSIIY-NSTTYTWSETYRRCLRLASSLSSI 60
           MEDLKPS ANS PLT +GF+ERAA +YGD  SI+Y NST YTW ET  RCL +AS+LSSI
Sbjct: 1   MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60

Query: 61  GIQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDARTIFVLLRHSNSKLIFVDQ 120
           GI R  VVSV++ NTP MYEL F+VPM GAILN IN RLDART+ VLLRH  SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120

Query: 121 ASCALIFEALALFPLETKRPLLVLIVDDTAMQDSPLPSFVSIHKDFVDSYEDMVQRGDSN 180
               L  EA+ +       P+LVLI ++   +     + V+    F   Y D++ RG+ +
Sbjct: 121 FYSDLAVEAITML---LNPPILVLIANEEEEEGG---AEVTERSKFCYLYSDLITRGNPD 180

Query: 181 FQWIRPTSEWDPIVLNYTSGTTSLPKGVVHCHRGIFTVTLDSLLEWGVPKQSVYLWTLPM 240
           F+WIRP SEWDPIV+NYTSGTTS PKGVVHCHRGIF +TLDSL +W VPK  VYLWTLP+
Sbjct: 181 FKWIRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPI 240

Query: 241 FHANGWSFPWGIAAVGGTNVCIRKFDATLIFSLIRRHHVTHLCGAPVVLNMLTNSPDN-R 300
           FHANGW++PWGIAAVGGTNVC+RK  A  I+ LIR H VTH+ GAP+VL +L+ S ++ +
Sbjct: 241 FHANGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQ 300

Query: 301 PLDRPVKILTAGAPPHATVLLRTESLGFVVTHGYGLTESGGTIICCSCKSEWNRLTATEQ 360
           PL  PV  LTAG+ P ATVLLR ESLGF+V+HGYGLTE+ G I+ C+ K  WNRL A++Q
Sbjct: 301 PLKSPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQ 360

Query: 361 ARLKARQGVRMLAVEAADVLDPNTRKSVKRDGASIGEIVVRGGSIMLGYFKDPEATSKAM 420
           A+LK+RQGVR +     DV+DP + +SV+RDG ++GEIV+RG SIMLGY K+P  T  + 
Sbjct: 361 AQLKSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNSF 420

Query: 421 TNDGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYMNPVVNEVAVVAR 480
            N GWFFTGD+GV+H DGYLEIKDRSKDVIISGGEN+SSVEVE++LY NP VNE AVVAR
Sbjct: 421 KN-GWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVAR 480

Query: 481 PDEFWGETPCAFVSLKKGLTRKPTEEEIIEYCKGKLPRFMVPKTVVFTTELPKTSTGKIK 540
           PDEFWGETPCAFVSLK GLTRKPT++EIIEYCK K+PR+M PKTV F  ELPKTSTGKI 
Sbjct: 481 PDEFWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKII 540

Query: 541 KFVLREMAKSM 550
           K +L+E+AK+M
Sbjct: 541 KSLLKEIAKNM 544

BLAST of Lsi09G008820 vs. TAIR10
Match: AT1G77240.1 (AT1G77240.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 699.9 bits (1805), Expect = 1.3e-201
Identity = 332/551 (60.25%), Postives = 424/551 (76.95%), Query Frame = 1

Query: 1   MEDLKPSPANSSPLTPIGFIERAAVIYGDSPSIIYNSTTYTWSETYRRCLRLASSLSS-- 60
           ME L P  +NS PLT +GF+ERAA ++GDSPS+++ +T +TWSET+ RCLR+AS+LSS  
Sbjct: 1   MELLLPHASNSCPLTVLGFLERAASVFGDSPSLLHTTTVHTWSETHSRCLRIASTLSSAS 60

Query: 61  IGIQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDARTIFVLLRHSNSKLIFVD 120
           +GI RG VVSVI PN P++YEL FAVPM GA+LN IN RLDA  + VLLRHS SKL+FVD
Sbjct: 61  LGINRGQVVSVIGPNVPSVYELQFAVPMSGAVLNNINPRLDAHALSVLLRHSESKLVFVD 120

Query: 121 QASCALIFEALALFPLETKRPLLVLIVDDTAMQDSPLPSFVSIHKDFVDSYEDMVQRGDS 180
             S +L+ EA++  P + +RP LV++ D   M     PS  S   DF+D+YE  ++RGD 
Sbjct: 121 HHSSSLVLEAVSFLP-KDERPRLVILNDGNDM-----PSSSSADMDFLDTYEGFMERGDL 180

Query: 181 NFQWIRPTSEWDPIVLNYTSGTTSLPKGVVHCHRGIFTVTLDSLLEWGVPKQSVYLWTLP 240
            F+W+RP SEW P+VLNYTSGTTS PKGVVH HR +F  T++SLL+W +P + VYLWTLP
Sbjct: 181 RFKWVRPKSEWTPMVLNYTSGTTSSPKGVVHSHRSVFMSTINSLLDWSLPNRPVYLWTLP 240

Query: 241 MFHANGWSFPWGIAAVGGTNVCIRKFDATLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNR 300
           MFHANGWS+ W  AAVG  N+C+ + D   IF+LI ++ VTH+C AP+VLNMLTN P  +
Sbjct: 241 MFHANGWSYTWATAAVGARNICVTRVDVPTIFNLIDKYQVTHMCAAPMVLNMLTNHPAQK 300

Query: 301 PLDRPVKILTAGAPPHATVLLRTESLGFVVTHGYGLTESGGTIICCSCKSEWNRLTATEQ 360
           PL  PVK++TAGAPP ATV+ + E+LGF V+HGYG+TE+GG ++ C+ K EW+RL   E+
Sbjct: 301 PLQSPVKVMTAGAPPPATVISKAEALGFDVSHGYGMTETGGLVVSCALKPEWDRLEPDER 360

Query: 361 ARLKARQGVRMLAVEAADVLDPNTRKSVKRDGASIGEIVVRGGSIMLGYFKDPEATSKAM 420
           A+ K+RQG+R       DV DP + KSVK DGA++GEIV RGGS+MLGY+KDPE T+ +M
Sbjct: 361 AKQKSRQGIRTAVFAEVDVRDPISGKSVKHDGATVGEIVFRGGSVMLGYYKDPEGTAASM 420

Query: 421 TNDGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYMNPVVNEVAVVAR 480
             DGWF+TGD+GVMHPDGYLE+KDRSKDV+I GGEN+SS E+E++LY NP + E AVVA+
Sbjct: 421 REDGWFYTGDIGVMHPDGYLEVKDRSKDVVICGGENISSTELEAVLYTNPAIKEAAVVAK 480

Query: 481 PDEFWGETPCAFVSLKKGLTRKPTEEEIIEYCKGKLPRFMVPKTVVFTTELPKTSTGKIK 540
           PD+ WGETPCAFVSLK       TE EI E+CK KLP++MVP+ VVF  ELPKTSTGKI+
Sbjct: 481 PDKMWGETPCAFVSLKYH-DGSVTEREIREFCKTKLPKYMVPRNVVFLEELPKTSTGKIQ 540

Query: 541 KFVLREMAKSM 550
           KF+LR+MAKS+
Sbjct: 541 KFLLRQMAKSL 544

BLAST of Lsi09G008820 vs. TAIR10
Match: AT1G21540.1 (AT1G21540.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 696.4 bits (1796), Expect = 1.4e-200
Identity = 340/558 (60.93%), Postives = 431/558 (77.24%), Query Frame = 1

Query: 1   MEDLKPSPANSSPLTPIGFIERAAVIYGDSPSIIYNSTT-YTWSETYRRCLRLASSL--S 60
           ME L P P+NS+PLT +GF++RAA +YGD PSI++ + T +TWSET+ RCLR+AS+L  S
Sbjct: 1   MELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTTNTVHTWSETHNRCLRIASALTSS 60

Query: 61  SIGIQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDARTIFVLLRHSNSKLIFV 120
           S+GI RG VVSV+ PN P++YEL FAVPM GAILN IN RLDA  + VLLRHS SKL+FV
Sbjct: 61  SLGINRGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESKLVFV 120

Query: 121 DQASCALIFEALALFPLETKRPLLVLIVDDTAMQDSPL-PSFVSIHKDFVDSYEDMVQRG 180
           D  S +++ EA++ F  + ++P LVL+ DD   +D  L PS  S   DF+D+Y+ +++RG
Sbjct: 121 DPNSISVVLEAVS-FMRQNEKPHLVLLDDD--QEDGSLSPSAAS---DFLDTYQGVMERG 180

Query: 181 DSNFQWIRPTSEWDPIVLNYTSGTTSLPKGVVHCHRGIFTVTLDSLLEWGVPKQSVYLWT 240
           DS F+WIRP +EW P++LNYTSGTTS PKGVV  HR IF +T+ SLL+W  P + VYLWT
Sbjct: 181 DSRFKWIRPQTEWQPMILNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWHFPNRPVYLWT 240

Query: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDATLIFSLIRRHHVTHLCGAPVVLNMLTNSPD 300
           LPMFHANGW + WG AAVG TNVC R+ DA  I+ LI +HHVTH+C AP+VLNMLTN P 
Sbjct: 241 LPMFHANGWGYTWGTAAVGATNVCTRRVDAPTIYDLIDKHHVTHMCAAPMVLNMLTNYPS 300

Query: 301 NRPLDRPVKILTAGAPPHATVLLRTESLGFVVTHGYGLTESGGTIICCSCKSEWNRLTAT 360
            +PL  PV+++TAGAPP A ++ R E+LGF V HGYGLTE+GG ++ C+ K+EW+ L   
Sbjct: 301 RKPLKNPVQVMTAGAPPPAAIISRAETLGFNVGHGYGLTETGGPVVSCAWKAEWDHLDPL 360

Query: 361 EQARLKARQGVRMLAVEAADVLDPNTRKSVKRDGASIGEIVVRGGSIMLGYFKDPEATSK 420
           E+ARLK+RQGVR +     DV DP T KSV+ DG S+GEIV++GGS+MLGY+KDPE T+ 
Sbjct: 361 ERARLKSRQGVRTIGFAEVDVRDPRTGKSVEHDGVSVGEIVLKGGSVMLGYYKDPEGTAA 420

Query: 421 AMTNDGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYMNPVVNEVAVV 480
            M  DGWF++GDVGV+H DGYLE+KDRSKDVII GGEN+SS EVE++LY NPVV E AVV
Sbjct: 421 CMREDGWFYSGDVGVIHEDGYLEVKDRSKDVIICGGENISSAEVETVLYTNPVVKEAAVV 480

Query: 481 ARPDEFWGETPCAFVSLK-----KGLTRKPTEEEIIEYCKGKLPRFMVPKTVVFTTELPK 540
           A+PD+ WGETPCAFVSLK      GL    TE EI E+CK +LP++MVP+ V+F  ELPK
Sbjct: 481 AKPDKMWGETPCAFVSLKYDSNGNGLV---TEREIREFCKTRLPKYMVPRKVIFQEELPK 540

Query: 541 TSTGKIKKFVLREMAKSM 550
           TSTGKI+KF+LR+MAKS+
Sbjct: 541 TSTGKIQKFLLRQMAKSL 549

BLAST of Lsi09G008820 vs. NCBI nr
Match: gi|659126420|ref|XP_008463174.1| (PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis melo])

HSP 1 Score: 1010.0 bits (2610), Expect = 1.6e-291
Identity = 494/557 (88.69%), Postives = 519/557 (93.18%), Query Frame = 1

Query: 1   MEDLKPSPANSSPLTPIGFIERAAVIYGDSPSIIYNSTTYTWSETYRRCLRLASSLSSIG 60
           MEDLKP+PANS PLTPIGFI+RAA IYGDS SIIYNSTTYTWSET+RRCL+LASS+SS G
Sbjct: 1   MEDLKPTPANSFPLTPIGFIQRAATIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDARTIFVLLRHSNSKLIFVDQA 120
           IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLD RTI +LLRHS SKLIFVDQA
Sbjct: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDTRTISILLRHSESKLIFVDQA 120

Query: 121 SCALIFEALALFPLETKRPLLVLIVDDTAMQDSPLPSFVSIHKDFVDSYEDMVQRGDSNF 180
           SCALIFEALALFP E KRPLLVLI DD A QDS LPSFVSI  +FVDSYEDMV+RGDS F
Sbjct: 121 SCALIFEALALFPPEMKRPLLVLIADDAAEQDSSLPSFVSIRDNFVDSYEDMVKRGDSKF 180

Query: 181 QWIRPTSEWDPIVLNYTSGTTSLPKGVVHCHRGIFTVTLDSLLEWGVPKQSVYLWTLPMF 240
           QWIRP SEWDPIVLNYTSGTTS PKGVVHCHRGIFTV LDSLLEWGVPKQSVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVALDSLLEWGVPKQSVYLWTLPMF 240

Query: 241 HANGWSFPWGIAAVGGTNVCIRKFDATLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRPL 300
           H NGWSFPWGIAAVGGTNVCIRKFDA LIFSLIRRHHVTHLCGAPVVLNMLTNSPDN PL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDARLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 301 DRPVKILTAGAPPHATVLLRTESLGFVVTHGYGLTESGGTIICCSCKSEWNRLTATEQAR 360
           DRPVKILTAGAPP A++LLRTESLGFVVTHGYGLTES GTIICCS KSEWN+L A EQAR
Sbjct: 301 DRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 361 LKARQGVRMLAVEAADVLDPNTRKSVKRDGASIGEIVVRGGSIMLGYFKDPEATSKAMTN 420
           LKARQGVRMLAVEA DVLDP TRKSVKRDGASIGEIVVRG S+ LGY KDPE T+KAM+ 
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGASVTLGYLKDPEGTAKAMSE 420

Query: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYMNPVVNEVAVVARPD 480
           DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVES+LYMNP+VNE AVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 481 EFWGETPCAFVSLKKGLTRKPTEEEIIEYCKGKLPRFMVPKTVVFTTELPKTSTGKIKKF 540
           EFWGETPCAF+SLK+GL+RKPTEEEIIEYCKGKLPRFMVPKTV+F TE+PKT+TGKI+KF
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQKF 540

Query: 541 VLREMAKSMGPTPKSRL 558
           VLR+ AKS+GP PKSRL
Sbjct: 541 VLRQKAKSLGPAPKSRL 557

BLAST of Lsi09G008820 vs. NCBI nr
Match: gi|449462358|ref|XP_004148908.1| (PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis sativus])

HSP 1 Score: 988.8 bits (2555), Expect = 3.9e-285
Identity = 483/557 (86.71%), Postives = 514/557 (92.28%), Query Frame = 1

Query: 1   MEDLKPSPANSSPLTPIGFIERAAVIYGDSPSIIYNSTTYTWSETYRRCLRLASSLSSIG 60
           MEDLKP PANS PLTPIGFIERAA+IYGDS SIIYNSTTYTWSET+RRCL+LASS+SS G
Sbjct: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDARTIFVLLRHSNSKLIFVDQA 120
           IQRGHVVSV APNTPAMYELHFAVPM GAILNTINLRLD RTI +LLRHS SKLIFVDQ 
Sbjct: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120

Query: 121 SCALIFEALALFPLETKRPLLVLIVDDTAMQDSPLPSFVSIHKDFVDSYEDMVQRGDSNF 180
           SCALIF+ALALFP + KRPLLVLI DDTA QDS LPS VSI  +FVDSYE+MV+ GDS F
Sbjct: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180

Query: 181 QWIRPTSEWDPIVLNYTSGTTSLPKGVVHCHRGIFTVTLDSLLEWGVPKQSVYLWTLPMF 240
           QWIRP SEWDPIVLNYTSGTTS PKGVV+CHRG+FTV LDS+LEWGVPKQSVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240

Query: 241 HANGWSFPWGIAAVGGTNVCIRKFDATLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRPL 300
           H NGWSFPWGIAAVGGTNVCIRKFD+ LIFSLIRRHHVTHLCGAPVVLNMLTNSPDN PL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 301 DRPVKILTAGAPPHATVLLRTESLGFVVTHGYGLTESGGTIICCSCKSEWNRLTATEQAR 360
           DRPVKILTAGAPP A+VLLRTESLGFVVTHGYGLTES GTIICCS KSEWN+L A EQAR
Sbjct: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 361 LKARQGVRMLAVEAADVLDPNTRKSVKRDGASIGEIVVRGGSIMLGYFKDPEATSKAMTN 420
           LKARQGVRMLAVEA DVLDP TRKSVKRDGASIGEIVVRG S+MLGY KD EATSKAM+ 
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420

Query: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYMNPVVNEVAVVARPD 480
           DGWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVES+LYMNP+VNE AVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 481 EFWGETPCAFVSLKKGLTRKPTEEEIIEYCKGKLPRFMVPKTVVFTTELPKTSTGKIKKF 540
           EFWGETPCAF+SLK+GL+RKPTEEEIIEYCKGKLP+FMVPKTV+F TE+PKT+TGK +KF
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540

Query: 541 VLREMAKSMGPTPKSRL 558
           VLR+ AKS+GP  KSRL
Sbjct: 541 VLRQKAKSLGPPQKSRL 557

BLAST of Lsi09G008820 vs. NCBI nr
Match: gi|449462360|ref|XP_004148909.1| (PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis sativus])

HSP 1 Score: 919.1 bits (2374), Expect = 3.8e-264
Identity = 456/557 (81.87%), Postives = 496/557 (89.05%), Query Frame = 1

Query: 1   MEDLKPSPANSSPLTPIGFIERAAVIYGDSPSIIYNSTTYTWSETYRRCLRLASSLSSIG 60
           ME LKP+PANSSPLTPIGF+ERA++IY DSPSIIY+STT+TWS+TYRRCLRLASSLSSIG
Sbjct: 22  MEHLKPTPANSSPLTPIGFLERASLIYADSPSIIYSSTTFTWSQTYRRCLRLASSLSSIG 81

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDARTIFVLLRHSNSKLIFVDQA 120
           I +GHVVSVIAPNTP MYEL FAVPM GAIL+ INLRLDARTI V LRHS SKL+FVDQA
Sbjct: 82  IHKGHVVSVIAPNTPPMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 141

Query: 121 SCALIFEALALFPLETKRPLLVLIVDDTAMQDSPLPSFVSIHKDFVDSYEDMVQRGDSNF 180
           S  LI +ALALFP E KRPLLVLI DD A++DS   S V+I  D VD YEDMVQ+GD  F
Sbjct: 142 SSGLISDALALFPPEAKRPLLVLISDDAAVEDSA--SSVAIGGDVVDLYEDMVQKGDPEF 201

Query: 181 QWIRPTSEWDPIVLNYTSGTTSLPKGVVHCHRGIFTVTLDSLLEWGVPKQSVYLWTLPMF 240
           +WIRP SEWDPIV+NYTSGTTS PKGVVH HRGIFTVTLDSL+EWGVPK SVYLWTLPMF
Sbjct: 202 RWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMF 261

Query: 241 HANGWSFPWGIAAVGGTNVCIRKFDATLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRPL 300
           HANGWSFP+ IAAVGGTN+CIRKFDA LIFSLI RHHVTH+CGAPVVLNMLTNSP+NRPL
Sbjct: 262 HANGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHMCGAPVVLNMLTNSPNNRPL 321

Query: 301 DRPVKILTAGAPPHATVLLRTESLGFVVTHGYGLTESGGTIICCSCKSEWNRLTATEQAR 360
           +RPVKILTAGAPP A VLLRTESLGF V+HGYGLTE+GG +I CS K EWN+L ATE+AR
Sbjct: 322 ERPVKILTAGAPPPAAVLLRTESLGFDVSHGYGLTETGGLVIFCSWKLEWNQLPATERAR 381

Query: 361 LKARQGVRMLAVEAADVLDPNTRKSVKRDGASIGEIVVRGGSIMLGYFKDPEATSKAMTN 420
           LKARQGVR LA+  ADVLD  T K V+RDG SIGEIV+RGGS+MLGYFKDPEAT KAMT 
Sbjct: 382 LKARQGVRTLAMAEADVLDSGTGKRVQRDGVSIGEIVIRGGSMMLGYFKDPEATKKAMTE 441

Query: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYMNPVVNEVAVVARPD 480
           +GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVES+LY NP VNE AVVARPD
Sbjct: 442 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 501

Query: 481 EFWGETPCAFVSLKKGLTRKPTEEEIIEYCKGKLPRFMVPKTVVFTTELPKTSTGKIKKF 540
           EFWGETPCAFVSL++GLTR PTEEEIIEYCKGKL  FMVPKTVVF  +LPKTSTGKI+KF
Sbjct: 502 EFWGETPCAFVSLREGLTRVPTEEEIIEYCKGKLAGFMVPKTVVFMEQLPKTSTGKIQKF 561

Query: 541 VLREMAKSMGPTPKSRL 558
           +LREMAKSMG +PKSRL
Sbjct: 562 LLREMAKSMGCSPKSRL 576

BLAST of Lsi09G008820 vs. NCBI nr
Match: gi|659126423|ref|XP_008463175.1| (PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis melo])

HSP 1 Score: 911.4 bits (2354), Expect = 7.9e-262
Identity = 448/557 (80.43%), Postives = 496/557 (89.05%), Query Frame = 1

Query: 1   MEDLKPSPANSSPLTPIGFIERAAVIYGDSPSIIYNSTTYTWSETYRRCLRLASSLSSIG 60
           M+ LKP+PANSSPLTPI F+ERA+++Y DSPSIIYNST++TWS+TY RCLRLASSLSS+G
Sbjct: 1   MDHLKPTPANSSPLTPISFLERASLVYADSPSIIYNSTSFTWSQTYLRCLRLASSLSSLG 60

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDARTIFVLLRHSNSKLIFVDQA 120
           I +GHVVSVI+PNTP MYEL F+VPM GAIL+ INLRLDARTI V LRHS SKL+FVDQA
Sbjct: 61  IHKGHVVSVISPNTPPMYELQFSVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 120

Query: 121 SCALIFEALALFPLETKRPLLVLIVDDTAMQDSPLPSFVSIHKDFVDSYEDMVQRGDSNF 180
           S ALI +AL LFP E+KRPLLVLI DD A++DS   S V+I  DFVD YEDM+Q+GD  F
Sbjct: 121 SSALISDALTLFPPESKRPLLVLIADDAAVEDSA--SSVAIGGDFVDFYEDMIQKGDPEF 180

Query: 181 QWIRPTSEWDPIVLNYTSGTTSLPKGVVHCHRGIFTVTLDSLLEWGVPKQSVYLWTLPMF 240
           +WIRP SEWDPIV+NYTSGTTS PKGVVH HRGIFTVTLDSL+EWGVPK SVYLWTLPMF
Sbjct: 181 RWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMF 240

Query: 241 HANGWSFPWGIAAVGGTNVCIRKFDATLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRPL 300
           HANGWSFP+ IAAVGGTN+CIRKFDA LIFSLI RHHVTHLCGAPVVLNMLTNSP+NRPL
Sbjct: 241 HANGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHLCGAPVVLNMLTNSPNNRPL 300

Query: 301 DRPVKILTAGAPPHATVLLRTESLGFVVTHGYGLTESGGTIICCSCKSEWNRLTATEQAR 360
           +RPVKILTAGAPP A VL RTESLGF V+HGYGLTE+GG +I CS K +WN+L ATE+AR
Sbjct: 301 ERPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQLPATERAR 360

Query: 361 LKARQGVRMLAVEAADVLDPNTRKSVKRDGASIGEIVVRGGSIMLGYFKDPEATSKAMTN 420
           LKARQGVR LA+  ADVLD  T KSVKRDG SIGEIV+RGGS+MLGYFKDPEAT KAMT 
Sbjct: 361 LKARQGVRTLAMAEADVLDSETGKSVKRDGVSIGEIVLRGGSMMLGYFKDPEATKKAMTE 420

Query: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYMNPVVNEVAVVARPD 480
           +GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVES+LY NP VNE AVVARPD
Sbjct: 421 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480

Query: 481 EFWGETPCAFVSLKKGLTRKPTEEEIIEYCKGKLPRFMVPKTVVFTTELPKTSTGKIKKF 540
           EFWGETPCAFVSL++GL+R PTEEEIIEYC+GKL  FMVPKTVVF  +LPKTSTGKI+KF
Sbjct: 481 EFWGETPCAFVSLREGLSRVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTSTGKIQKF 540

Query: 541 VLREMAKSMGPTPKSRL 558
           +LREMAKSMGP+ KSRL
Sbjct: 541 LLREMAKSMGPSRKSRL 555

BLAST of Lsi09G008820 vs. NCBI nr
Match: gi|1009151390|ref|XP_015893528.1| (PREDICTED: probable acyl-activating enzyme 6 [Ziziphus jujuba])

HSP 1 Score: 837.0 bits (2161), Expect = 1.9e-239
Identity = 404/560 (72.14%), Postives = 472/560 (84.29%), Query Frame = 1

Query: 1   MEDLKPSPANSSPLTPIGFIERAAVIYGDSPSIIYNSTTYTWSETYRRCLRLASSLSSIG 60
           MEDLKP+P NSSPLTPIGF+ERAA +YGD PSI+YN+TTYTWS+T+ RCLR+ASS+ S+G
Sbjct: 1   MEDLKPTPPNSSPLTPIGFLERAATVYGDCPSIVYNTTTYTWSQTHLRCLRVASSIQSLG 60

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDARTIFVLLRHSNSKLIFVDQA 120
           I++GHVVSV+APN PAMYELHFA  M GAILN INLRLD RT+ +LLRHS SKL+FVDQ 
Sbjct: 61  IKKGHVVSVLAPNIPAMYELHFAALMSGAILNNINLRLDPRTVSILLRHSESKLVFVDQL 120

Query: 121 SCALIFEALALFPLETKRPLLVLIVDDTAMQDSPLPSFVSIHKDFVDSYEDMVQRGDSNF 180
           S +LI EAL+LFP     P L+LI DDT    S   S  S   +FV SYED+V+RGD  F
Sbjct: 121 SQSLIHEALSLFPPGIPIPRLILIADDTDEASSSPSSSSSPAVEFVCSYEDLVERGDPGF 180

Query: 181 QWIRPTSEWDPIVLNYTSGTTSLPKGVVHCHRGIFTVTLDSLLEWGVPKQSVYLWTLPMF 240
           +WIRPTSEWDP +LNYTSGTTS PKGVVHC RG+F +T+DSL++W VPKQ VYLWTLPMF
Sbjct: 181 EWIRPTSEWDPAILNYTSGTTSSPKGVVHCQRGLFLITVDSLIDWAVPKQPVYLWTLPMF 240

Query: 241 HANGWSFPWGIAAVGGTNVCIRKFDATLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRPL 300
           HANGWSFPWG+AAVGGTN+C+RKFDAT+I+ LIR H+VTH+CGAPVVLNML+NSPDN PL
Sbjct: 241 HANGWSFPWGMAAVGGTNICLRKFDATIIYRLIRAHNVTHMCGAPVVLNMLSNSPDNEPL 300

Query: 301 DRPVKILTAGAPPHATVLLRTESLGFVVTHGYGLTESGGTIICCSCKSEWNRLTATEQAR 360
             PV ILTAGAPP A VL RTESLGFVV+HGYGLTE+ G ++ C+ K EWNRL A+E+AR
Sbjct: 301 KNPVNILTAGAPPPAAVLHRTESLGFVVSHGYGLTETAGLVVSCAWKREWNRLPASERAR 360

Query: 361 LKARQGVRMLAVEAADVLDPNTRKSVKRDGASIGEIVVRGGSIMLGYFKDPEATSKAMTN 420
           LKARQGVR+  +   DVLDPNT KSVKRDG+++GEIV+RGG +MLGY KDP  T+K M  
Sbjct: 361 LKARQGVRLAGMTRIDVLDPNTGKSVKRDGSTLGEIVLRGGCVMLGYLKDPAGTAKCM-K 420

Query: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYMNPVVNEVAVVARPD 480
           DGWFFTGDV VMHPDGYLEIKDRSKDVIISGGENLSSVEVES+LYM+P +NE AVVARPD
Sbjct: 421 DGWFFTGDVAVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMHPAINEAAVVARPD 480

Query: 481 EFWGETPCAFVSLKKGLT-RKPTEEEIIEYCKGKLPRFMVPKTVVFTTELPKTSTGKIKK 540
           +FWGETPCAFVSLK+ +   KP+E+EI+E+C+ KLP +MVPKTVVF  ELPKTSTGKI+K
Sbjct: 481 DFWGETPCAFVSLKERIRGNKPSEKEIMEFCREKLPHYMVPKTVVFKEELPKTSTGKIQK 540

Query: 541 FVLREMAKSMGP--TPKSRL 558
           F+LRE AKSMG   T +SR+
Sbjct: 541 FILRETAKSMGSAGTRRSRI 559