Lsi09G006980 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi09G006980
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Vacuolar iron transporter 1-like protein
Location	chr09 : 7921481 .. 7921681 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTTTTCTCAAAAGCCTCAGAGGCTGTGGTTGCATCTGTTGCCTTAACTTTGGTGGCACTCTTAGTTTTTGGCTACGCCAAGGGTTACTTCACCGGCAATAGACCCGTCATGAGTGCCGTCCAAACCGCCCTCATCGGAGCCATAGCGTCATCGGCCGCCTTCGGCATGGCCAAGGCTGTCCAACCACGTCAACCCTAA

mRNA sequence

ATGTTTTTCTCAAAAGCCTCAGAGGCTGTGGTTGCATCTGTTGCCTTAACTTTGGTGGCACTCTTAGTTTTTGGCTACGCCAAGGGTTACTTCACCGGCAATAGACCCGTCATGAGTGCCGTCCAAACCGCCCTCATCGGAGCCATAGCGTCATCGGCCGCCTTCGGCATGGCCAAGGCTGTCCAACCACGTCAACCCTAA

Coding sequence (CDS)

ATGTTTTTCTCAAAAGCCTCAGAGGCTGTGGTTGCATCTGTTGCCTTAACTTTGGTGGCACTCTTAGTTTTTGGCTACGCCAAGGGTTACTTCACCGGCAATAGACCCGTCATGAGTGCCGTCCAAACCGCCCTCATCGGAGCCATAGCGTCATCGGCCGCCTTCGGCATGGCCAAGGCTGTCCAACCACGTCAACCCTAA

Protein sequence

MFFSKASEAVVASVALTLVALLVFGYAKGYFTGNRPVMSAVQTALIGAIASSAAFGMAKAVQPRQP

Homology

[Close]

BLAST of Lsi09G006980 vs. Swiss-Prot
Match: VIT11_ORYSJ (Vacuolar iron transporter 1.1 OS=Oryza sativa subsp. japonica GN=VIT1.1 PE=2 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 4.1e-16
Identity = 45/64 (70.31%), Postives = 53/64 (82.81%), Query Frame = 1


Query: 1   MFFSKASEAVVASVALTLVALLVFGYAKGYFTGNRPVMSAVQTALIGAIASSAAFGMAKA 60
           MF S A  A++ SV +TLVALL FGY KG FTGNRP +SAVQTA+IGA+AS+AA+GMAKA
Sbjct: 189 MFISTAQNAMLTSVGVTLVALLFFGYIKGRFTGNRPFLSAVQTAIIGALASAAAYGMAKA 248

Query: 61  VQPR 65
           VQ R
Sbjct: 249 VQTR 252

[Close]

BLAST of Lsi09G006980 vs. Swiss-Prot
Match: VIT12_ORYSJ (Vacuolar iron transporter 1.2 OS=Oryza sativa subsp. japonica GN=VIT1.2 PE=3 SV=2)

HSP 1 Score: 77.8 bits (190), Expect = 5.0e-14
Identity = 42/62 (67.74%), Postives = 48/62 (77.42%), Query Frame = 1


Query: 1   MFFSKASEAVVASVALTLVALLVFGYAKGYFTGNRPVMSAVQTALIGAIASSAAFGMAKA 60
           MF   A  A+  SV +TL ALL FGY KG FTGNRP +SA QTA+IGA+AS+AAFGMAKA
Sbjct: 183 MFVPTADRAMATSVVVTLAALLFFGYVKGRFTGNRPFISAFQTAVIGALASAAAFGMAKA 242

Query: 61  VQ 63
           VQ
Sbjct: 243 VQ 244

[Close]

BLAST of Lsi09G006980 vs. Swiss-Prot
Match: VIT1_ARATH (Vacuolar iron transporter 1 OS=Arabidopsis thaliana GN=VIT1 PE=2 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 9.3e-13
Identity = 39/62 (62.90%), Postives = 49/62 (79.03%), Query Frame = 1


Query: 1   MFFSKASEAVVASVALTLVALLVFGYAKGYFTGNRPVMSAVQTALIGAIASSAAFGMAKA 60
           M    A +AVVASV +TL AL +FGYAKG+FTG++P+ SA +TA IGAIAS+AAF +AK 
Sbjct: 188 MLIPHAMDAVVASVVITLFALFIFGYAKGHFTGSKPLRSAFETAFIGAIASAAAFCLAKV 247

Query: 61  VQ 63
           VQ
Sbjct: 248 VQ 249

[Close]

BLAST of Lsi09G006980 vs. TrEMBL
Match: A0A0A0KSS5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G550250 PE=4 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 6.9e-23
Identity = 59/66 (89.39%), Postives = 65/66 (98.48%), Query Frame = 1


Query: 1   MFFSKASEAVVASVALTLVALLVFGYAKGYFTGNRPVMSAVQTALIGAIASSAAFGMAKA 60
           MFF KASEAV+AS+ALTLVALLVFGYAKGYFTGN+PVMSAVQTALIGAIAS+AA+GMAKA
Sbjct: 181 MFFPKASEAVLASIALTLVALLVFGYAKGYFTGNKPVMSAVQTALIGAIASAAAYGMAKA 240

Query: 61  VQPRQP 67
           +QPRQP
Sbjct: 241 IQPRQP 246

[Close]

BLAST of Lsi09G006980 vs. TrEMBL
Match: V4U611_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10016475mg PE=4 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 2.2e-16
Identity = 48/61 (78.69%), Postives = 57/61 (93.44%), Query Frame = 1


Query: 1   MFFSKASEAVVASVALTLVALLVFGYAKGYFTGNRPVMSAVQTALIGAIASSAAFGMAKA 60
           MF  +A++AV+ASVA+TLVALL+FGYAKGYFTGN+PV SA+QTA IGAIAS+AAFGMAKA
Sbjct: 179 MFIPRATDAVLASVAVTLVALLIFGYAKGYFTGNKPVKSALQTAFIGAIASAAAFGMAKA 238

Query: 61  V 62
           V
Sbjct: 239 V 239

[Close]

BLAST of Lsi09G006980 vs. TrEMBL
Match: A0A067F3X4_CITSI (Uncharacterized protein (Fragment) OS=Citrus sinensis GN=CISIN_1g0263512mg PE=4 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 2.2e-16
Identity = 48/61 (78.69%), Postives = 57/61 (93.44%), Query Frame = 1


Query: 1   MFFSKASEAVVASVALTLVALLVFGYAKGYFTGNRPVMSAVQTALIGAIASSAAFGMAKA 60
           MF  +A++AV+ASVA+TLVALL+FGYAKGYFTGN+PV SA+QTA IGAIAS+AAFGMAKA
Sbjct: 103 MFIPRATDAVLASVAVTLVALLIFGYAKGYFTGNKPVKSALQTAFIGAIASAAAFGMAKA 162

Query: 61  V 62
           V
Sbjct: 163 V 163

[Close]

BLAST of Lsi09G006980 vs. TrEMBL
Match: A0A0S3QWI8_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.01G013600 PE=4 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 2.8e-16
Identity = 49/63 (77.78%), Postives = 56/63 (88.89%), Query Frame = 1


Query: 1   MFFSKASEAVVASVALTLVALLVFGYAKGYFTGNRPVMSAVQTALIGAIASSAAFGMAKA 60
           MF ++ASEAVV SV +TLVALLVFGYAKGYFTGN+P  SA++TALIGAIAS+AAFGMAKA
Sbjct: 185 MFIARASEAVVFSVIVTLVALLVFGYAKGYFTGNKPFRSALETALIGAIASAAAFGMAKA 244

Query: 61  VQP 64
             P
Sbjct: 245 FNP 247

[Close]

BLAST of Lsi09G006980 vs. TrEMBL
Match: A0A0L9T931_PHAAN (Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan325s004800 PE=4 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 2.8e-16
Identity = 49/63 (77.78%), Postives = 56/63 (88.89%), Query Frame = 1


Query: 1   MFFSKASEAVVASVALTLVALLVFGYAKGYFTGNRPVMSAVQTALIGAIASSAAFGMAKA 60
           MF ++ASEAVV SV +TLVALLVFGYAKGYFTGN+P  SA++TALIGAIAS+AAFGMAKA
Sbjct: 185 MFIARASEAVVFSVIVTLVALLVFGYAKGYFTGNKPFRSALETALIGAIASAAAFGMAKA 244

Query: 61  VQP 64
             P
Sbjct: 245 FNP 247

[Close]

BLAST of Lsi09G006980 vs. TAIR10
Match: AT2G01770.1 (AT2G01770.1 vacuolar iron transporter 1)

HSP 1 Score: 73.6 bits (179), Expect = 5.3e-14
Identity = 39/62 (62.90%), Postives = 49/62 (79.03%), Query Frame = 1


Query: 1   MFFSKASEAVVASVALTLVALLVFGYAKGYFTGNRPVMSAVQTALIGAIASSAAFGMAKA 60
           M    A +AVVASV +TL AL +FGYAKG+FTG++P+ SA +TA IGAIAS+AAF +AK 
Sbjct: 188 MLIPHAMDAVVASVVITLFALFIFGYAKGHFTGSKPLRSAFETAFIGAIASAAAFCLAKV 247

Query: 61  VQ 63
           VQ
Sbjct: 248 VQ 249

[Close]

BLAST of Lsi09G006980 vs. NCBI nr
Match: gi|449450149|ref|XP_004142826.1| (PREDICTED: vacuolar iron transporter 1.1 [Cucumis sativus])

HSP 1 Score: 114.0 bits (284), Expect = 1.0e-22
Identity = 59/66 (89.39%), Postives = 65/66 (98.48%), Query Frame = 1


Query: 1   MFFSKASEAVVASVALTLVALLVFGYAKGYFTGNRPVMSAVQTALIGAIASSAAFGMAKA 60
           MFF KASEAV+AS+ALTLVALLVFGYAKGYFTGN+PVMSAVQTALIGAIAS+AA+GMAKA
Sbjct: 181 MFFPKASEAVLASIALTLVALLVFGYAKGYFTGNKPVMSAVQTALIGAIASAAAYGMAKA 240

Query: 61  VQPRQP 67
           +QPRQP
Sbjct: 241 IQPRQP 246

[Close]

BLAST of Lsi09G006980 vs. NCBI nr
Match: gi|659072768|ref|XP_008467051.1| (PREDICTED: vacuolar iron transporter 1.1-like [Cucumis melo])

HSP 1 Score: 110.2 bits (274), Expect = 1.4e-21
Identity = 58/66 (87.88%), Postives = 63/66 (95.45%), Query Frame = 1


Query: 1   MFFSKASEAVVASVALTLVALLVFGYAKGYFTGNRPVMSAVQTALIGAIASSAAFGMAKA 60
           MFF KASEAV+ASVALTLVALLVFGYAKGYFTGN+P  SAVQTALIGAIAS+AA+GMAKA
Sbjct: 181 MFFPKASEAVLASVALTLVALLVFGYAKGYFTGNKPFTSAVQTALIGAIASAAAYGMAKA 240

Query: 61  VQPRQP 67
           +QPRQP
Sbjct: 241 IQPRQP 246

[Close]

BLAST of Lsi09G006980 vs. NCBI nr
Match: gi|641841890|gb|KDO60800.1| (hypothetical protein CISIN_1g0263512mg, partial [Citrus sinensis])

HSP 1 Score: 92.4 bits (228), Expect = 3.1e-16
Identity = 48/61 (78.69%), Postives = 57/61 (93.44%), Query Frame = 1


Query: 1   MFFSKASEAVVASVALTLVALLVFGYAKGYFTGNRPVMSAVQTALIGAIASSAAFGMAKA 60
           MF  +A++AV+ASVA+TLVALL+FGYAKGYFTGN+PV SA+QTA IGAIAS+AAFGMAKA
Sbjct: 103 MFIPRATDAVLASVAVTLVALLIFGYAKGYFTGNKPVKSALQTAFIGAIASAAAFGMAKA 162

Query: 61  V 62
           V
Sbjct: 163 V 163

[Close]

BLAST of Lsi09G006980 vs. NCBI nr
Match: gi|567911647|ref|XP_006448137.1| (hypothetical protein CICLE_v10016475mg [Citrus clementina])

HSP 1 Score: 92.4 bits (228), Expect = 3.1e-16
Identity = 48/61 (78.69%), Postives = 57/61 (93.44%), Query Frame = 1


Query: 1   MFFSKASEAVVASVALTLVALLVFGYAKGYFTGNRPVMSAVQTALIGAIASSAAFGMAKA 60
           MF  +A++AV+ASVA+TLVALL+FGYAKGYFTGN+PV SA+QTA IGAIAS+AAFGMAKA
Sbjct: 179 MFIPRATDAVLASVAVTLVALLIFGYAKGYFTGNKPVKSALQTAFIGAIASAAAFGMAKA 238

Query: 61  V 62
           V
Sbjct: 239 V 239

[Close]

BLAST of Lsi09G006980 vs. NCBI nr
Match: gi|659129452|ref|XP_008464694.1| (PREDICTED: vacuolar iron transporter 1-like [Cucumis melo])

HSP 1 Score: 92.4 bits (228), Expect = 3.1e-16
Identity = 49/62 (79.03%), Postives = 53/62 (85.48%), Query Frame = 1


Query: 1   MFFSKASEAVVASVALTLVALLVFGYAKGYFTGNRPVMSAVQTALIGAIASSAAFGMAKA 60
           MF S    AV ASVALTLVALLVFGYAKGYFTGN+P  SA+QT LIGAIAS+AAFGMAKA
Sbjct: 189 MFISNVKRAVTASVALTLVALLVFGYAKGYFTGNKPFKSAIQTTLIGAIASAAAFGMAKA 248

Query: 61  VQ 63
           +Q
Sbjct: 249 IQ 250

The following BLAST results are available for this feature:

BLAST of Lsi09G006980 vs. Swiss-Prot


Match Name	E-value	Identity	Description
VIT11_ORYSJ	4.1e-16	70.31	Vacuolar iron transporter 1.1 OS=Oryza sativa subsp. japonica GN=VIT1.1 PE=2 SV=...	[more]
VIT12_ORYSJ	5.0e-14	67.74	Vacuolar iron transporter 1.2 OS=Oryza sativa subsp. japonica GN=VIT1.2 PE=3 SV=...	[more]
VIT1_ARATH	9.3e-13	62.90	Vacuolar iron transporter 1 OS=Arabidopsis thaliana GN=VIT1 PE=2 SV=1	[more]

BLAST of Lsi09G006980 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KSS5_CUCSA	6.9e-23	89.39	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G550250 PE=4 SV=1	[more]
V4U611_9ROSI	2.2e-16	78.69	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10016475mg PE=4 SV=1	[more]
A0A067F3X4_CITSI	2.2e-16	78.69	Uncharacterized protein (Fragment) OS=Citrus sinensis GN=CISIN_1g0263512mg PE=4 ...	[more]
A0A0S3QWI8_PHAAN	2.8e-16	77.78	Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.01G013600 PE=...	[more]
A0A0L9T931_PHAAN	2.8e-16	77.78	Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan325s004800 PE=4 SV=1	[more]

BLAST of Lsi09G006980 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G01770.1	5.3e-14	62.90	vacuolar iron transporter 1	[more]

BLAST of Lsi09G006980 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449450149\|ref\|XP_004142826.1\|	1.0e-22	89.39	PREDICTED: vacuolar iron transporter 1.1 [Cucumis sativus]	[more]
gi\|659072768\|ref\|XP_008467051.1\|	1.4e-21	87.88	PREDICTED: vacuolar iron transporter 1.1-like [Cucumis melo]	[more]
gi\|641841890\|gb\|KDO60800.1\|	3.1e-16	78.69	hypothetical protein CISIN_1g0263512mg, partial [Citrus sinensis]	[more]
gi\|567911647\|ref\|XP_006448137.1\|	3.1e-16	78.69	hypothetical protein CICLE_v10016475mg [Citrus clementina]	[more]
gi\|659129452\|ref\|XP_008464694.1\|	3.1e-16	79.03	PREDICTED: vacuolar iron transporter 1-like [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR008217	Ccc1_fam

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030026	cellular manganese ion homeostasis
biological_process	GO:0071281	cellular response to iron ion
biological_process	GO:0006880	intracellular sequestering of iron ion
biological_process	GO:0034755	iron ion transmembrane transport
biological_process	GO:0071421	manganese ion transmembrane transport
biological_process	GO:0008150	biological_process
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005774	vacuolar membrane
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005381	iron ion transmembrane transporter activity
molecular_function	GO:0005384	manganese ion transmembrane transporter activity
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi09G006980.1	Lsi09G006980.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008217	Ccc1 family	PFAM	PF01988	VIT1	coord: 1..58 score: 1.2
None	No IPR available	PANTHER	PTHR31851	FAMILY NOT NAMED	coord: 6..62 score: 6.4
None	No IPR available	PANTHER	PTHR31851:SF9	SUBFAMILY NOT NAMED	coord: 6..62 score: 6.4

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi09G006980	Csa5G550240	Cucumber (Chinese Long) v2	culsiB329
Lsi09G006980	CSPI05G19170	Wild cucumber (PI 183967)	cpilsiB329
Lsi09G006980	Cucsa.012090	Cucumber (Gy14) v1	cgylsiB316
Lsi09G006980	CmaCh04G021350	Cucurbita maxima (Rimu)	cmalsiB622
Lsi09G006980	CmoCh04G022280	Cucurbita moschata (Rifu)	cmolsiB610
Lsi09G006980	Cla005800	Watermelon (97103) v1	lsiwmB028
Lsi09G006980	MELO3C005260	Melon (DHL92) v3.5.1	lsimeB013
Lsi09G006980	ClCG01G006640	Watermelon (Charleston Gray)	lsiwcgB008
Lsi09G006980	MELO3C005259.2	Melon (DHL92) v3.6.1	lsimedB008
Lsi09G006980	Cla97C01G006930	Watermelon (97103) v2	lsiwmbB007
Lsi09G006980	Bhi12G000836	Wax gourd	lsiwgoB006
Lsi09G006980	Cp4.1LG13g06660	Cucurbita pepo (Zucchini)	cpelsiB130
Lsi09G006980	CsGy5G018610	Cucumber (Gy14) v2	cgyblsiB301
Lsi09G006980	Carg25246	Silver-seed gourd	carlsiB799
Lsi09G006980	CsaV3_5G027930	Cucumber (Chinese Long) v3	cuclsiB352

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi09G006980	Cucurbita maxima (Rimu)	cmalsiB268
Lsi09G006980	Cucurbita moschata (Rifu)	cmolsiB256
Lsi09G006980	Cucurbita pepo (Zucchini)	cpelsiB311
Lsi09G006980	Melon (DHL92) v3.5.1	lsimeB008