Lsi09G006650 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi09G006650
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Calcium-dependent lipid-binding domain-containing protein
Location	chr09 : 7443141 .. 7445538 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TAATAAAATGTTTAAATTGAAGGGACCTGACCGGCGAAACTACAGTTTAGCGCGGCGACAACGATGGTCGACCACAAAGAAAATCCTGAGTTCCGACCACCCACTTCTTCAATCTTCTTCTTCATTTCTGTATTTCTCTCTTCTTTTAACTCATCGTTAGGGTTTTGAAACTCTGATTTTTCCACTCCGTGTAAGTCAAATTTTCTCTTTAAGTTCGTACTCCAATTATTTATATCTCATTACCTTCTCGATTGAGGTCTATTTTCCTCCACGATGGAACACTTTTTGGGTCTTCTCCGAATTCGGGTGTTGCACGGTGTTAATCTCGCTATTCGTGATTCCCGTAGCAGCGATCCTTATGTCGTCGTCACAATGGGCGAGCAGGTCTCTGGATTTTCATTTCTTACTAGAATTTTCCTGTTTTTTATGAGATTGAAGTGTGCTTTATGCTTAAGTTTCTTGAATCAGTGCATATTAATTTAGCTCGTTTTAGGTTTATTCATTATTCAGTTGTTCTCTGTTCCCTTCTTCCCCCGTGCGATCGTCCATCTCCTTGACCTAGATTGTTTGCTTTTAAATTCAGACTAACAGAAGAGGCTTGAATAGTTTTGAAAAGAAAGGATTAGAATAACTGATTTGATCGATATCACTTTGAAAAATGGTATTAAATATCATTTCGATTTTGAGTTTGTCGATGACCTTTCATGGGCCTAGCTGTTCTGAGCTTAAGAAGAGGGGGATCATCTGTGCATTATCTTTTTGTGGCAGTTGGAGGTTTTCTTGTTATTTTTGTAAACAATTTTTCTGATTTTTAGTTGGGTATGTTGCTGATTTGATATTTGTCTAAAATGGAGGCTTTTTTTGTATTTATTCGTTATTTTTCGATTTTGGGGATCTCTATACGATTGTAGGAAGTTCGTATCTTTGAATACTTATTTAACTTTTTAACTCTTGATGAAAAGTATGCTTCTGGTTTTGGAAAAAATGCGTTTTGTGAATGCTTGATAATTTATAATAACAGGAAACATTGGAAGAAGCAGAAAATCAAGACAACGGGAATTGTGTAGCCTAACTTATTTTCATTTTTTTGCTTTTCTTGAATCAAATGACTTTGGTCTTTGCTTGATTATTCTTTGTGCCATCATTTTTCTTTTTCTTTTTTGCCAAGGATAACAGGTAAATCTATGGTAAGATCGGTTGATCCAACTTACTCTTGGTCTACTTTTATGAGGTATTTGATTCGTGTGAGGGTTGGGACATTAAGCCTAAATTCAAAACATTGGATTGTTTTATCGTAGAGCTCGACGATTTGCTAATCCGATGAAATGATAATGTTACCAAGTCTTGAGATTCATCTCTCAGGATTATTACTCGAGAGAAATTTATAAACCAAACATACGACACACAGAATTAAGTTTCTAATGTTCATCTCTAAACACATAATTCTAGGTTTCCTAATTAACTATGAAAACTCAAGCTTTCTAAATAATGAACCAAAGCCCACCTTACTGATACTCTTGAGCCTTCTATTTTGAATCTTGAATGATTTTTTTTTCTTATTCTTCTGACATAACTCAAATTGGTGCAGAGGTTGAAGACCCGGGTGGTGAAAAATAATTGCAATCCTGAATGGAATGATGAGTTGACTCTTTCAGTTGCAGATCTCAGGACTCCAATCGGTTTAGTAAGTAATATTTCTCTTCAATTATTAGGAATAAGAGCTTCAAGCCAACCACCTGAAGAGGAAAGGAGATATATTCTATGTACAATGAAGAAAATGAGCATAATGAGCATGTGTAACTTACTGCTTTATGTGCTCTTGTTTTTCCCTTTTTGTAGTCAGTGTATGACAAAGACACACTAACAGACCATGACAAAATGGGAGATGCCGAGATCGACATTGAACCGTACATCGAGTGTTTGAAGATGGGACTGGAGAGCCTCCCAGATGGGTGTGTGGTTAAGAGAGTATATCCAAAAAGAACAAACAATTTAGCTGATGAGAGCCAATGTGTTTGGCAGAAGGGGAAAATTGTACAGAACATGTTCCTGAGGTTGAAGAATGTAGAGTGTGGTGAGGTGGCAATTCAGCTCGAATGGATCGACGTTCCTGGTGCTAAGGGTCTGCCGATTGGGGGTACGAGCCACTTCTGATTTCTTAACTGATGCACCAGGTAACTAAAAATTTCCCAGTTCCATGACTTGCAGATTATGTGAAATAAAATAAAGATGTTTGCCTTGTGACCTGAAACTGGATTTAGAGTTGTGGGTCTGTAGTATGAATACAACTCTGGATTGGGTGTAGTGGATGGGGCTGGGGCTGGGTTAGTGAAACTGCTCCCGGTTAGTGAAACTGCTCCCTGTAATCATAAATATTACTATGGTTGTTTGTTGGCAG

mRNA sequence

TAATAAAATGTTTAAATTGAAGGGACCTGACCGGCGAAACTACAGTTTAGCGCGGCGACAACGATGGTCGACCACAAAGAAAATCCTGAGTTCCGACCACCCACTTCTTCAATCTTCTTCTTCATTTCTGTATTTCTCTCTTCTTTTAACTCATCGTTAGGGTTTTGAAACTCTGATTTTTCCACTCCGTGTAAGTCAAATTTTCTCTTTAAGTTCGTACTCCAATTATTTATATCTCATTACCTTCTCGATTGAGGTCTATTTTCCTCCACGATGGAACACTTTTTGGGTCTTCTCCGAATTCGGGTGTTGCACGGTGTTAATCTCGCTATTCGTGATTCCCGTAGCAGCGATCCTTATGTCGTCGTCACAATGGGCGAGCAGATTGAAGTGTGCTTTATGCTTAAGTTTCTTGAATCAGTGCATATTAATTTAGCTCGTTTTAGGTTTATTCATTATTCAGTTGTTCTCTGTTCCCTTCTTCCCCCTTGGGAAGTTCGTATCTTTGAATACTTATTTAACTTTTTAACTCTTGATGAAAAGATAACAGGTAAATCTATGGTAAGATCGGTTGATCCAACTTACTCTTGGTCTACTTTTATGAGGTATTTGATTCGTAGGTTGAAGACCCGGGTGGTGAAAAATAATTGCAATCCTGAATGGAATGATGAGTTGACTCTTTCAGTTGCAGATCTCAGGACTCCAATCGGTTTATCAGTGTATGACAAAGACACACTAACAGACCATGACAAAATGGGAGATGCCGAGATCGACATTGAACCGTACATCGAGTGTTTGAAGATGGGACTGGAGAGCCTCCCAGATGGGTGTGTGGTTAAGAGAGTATATCCAAAAAGAACAAACAATTTAGCTGATGAGAGCCAATGTGTTTGGCAGAAGGGGAAAATTGTACAGAACATGTTCCTGAGGTTGAAGAATGTAGAGTGTGGTGAGGTGGCAATTCAGCTCGAATGGATCGACGTTCCTGGTGCTAAGGGTCTGCCGATTGGGGGTACGAGCCACTTCTGATTTCTTAACTGATGCACCAGGTAACTAAAAATTTCCCAGTTCCATGACTTGCAGATTATGTGAAATAAAATAAAGATGTTTGCCTTGTGACCTGAAACTGGATTTAGAGTTGTGGGTCTGTAGTATGAATACAACTCTGGATTGGGTGTAGTGGATGGGGCTGGGGCTGGGTTAGTGAAACTGCTCCCGGTTAGTGAAACTGCTCCCTGTAATCATAAATATTACTATGGTTGTTTGTTGGCAG

Coding sequence (CDS)

ATGGAACACTTTTTGGGTCTTCTCCGAATTCGGGTGTTGCACGGTGTTAATCTCGCTATTCGTGATTCCCGTAGCAGCGATCCTTATGTCGTCGTCACAATGGGCGAGCAGATTGAAGTGTGCTTTATGCTTAAGTTTCTTGAATCAGTGCATATTAATTTAGCTCGTTTTAGGTTTATTCATTATTCAGTTGTTCTCTGTTCCCTTCTTCCCCCTTGGGAAGTTCGTATCTTTGAATACTTATTTAACTTTTTAACTCTTGATGAAAAGATAACAGGTAAATCTATGGTAAGATCGGTTGATCCAACTTACTCTTGGTCTACTTTTATGAGGTATTTGATTCGTAGGTTGAAGACCCGGGTGGTGAAAAATAATTGCAATCCTGAATGGAATGATGAGTTGACTCTTTCAGTTGCAGATCTCAGGACTCCAATCGGTTTATCAGTGTATGACAAAGACACACTAACAGACCATGACAAAATGGGAGATGCCGAGATCGACATTGAACCGTACATCGAGTGTTTGAAGATGGGACTGGAGAGCCTCCCAGATGGGTGTGTGGTTAAGAGAGTATATCCAAAAAGAACAAACAATTTAGCTGATGAGAGCCAATGTGTTTGGCAGAAGGGGAAAATTGTACAGAACATGTTCCTGAGGTTGAAGAATGTAGAGTGTGGTGAGGTGGCAATTCAGCTCGAATGGATCGACGTTCCTGGTGCTAAGGGTCTGCCGATTGGGGGTACGAGCCACTTCTGA

Protein sequence

MEHFLGLLRIRVLHGVNLAIRDSRSSDPYVVVTMGEQIEVCFMLKFLESVHINLARFRFIHYSVVLCSLLPPWEVRIFEYLFNFLTLDEKITGKSMVRSVDPTYSWSTFMRYLIRRLKTRVVKNNCNPEWNDELTLSVADLRTPIGLSVYDKDTLTDHDKMGDAEIDIEPYIECLKMGLESLPDGCVVKRVYPKRTNNLADESQCVWQKGKIVQNMFLRLKNVECGEVAIQLEWIDVPGAKGLPIGGTSHF

Homology

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BLAST of Lsi09G006650 vs. Swiss-Prot
Match: CAR7_ARATH (Protein C2-DOMAIN ABA-RELATED 7 OS=Arabidopsis thaliana GN=CAR7 PE=1 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 1.9e-45
Identity = 84/129 (65.12%), Postives = 102/129 (79.07%), Query Frame = 1


Query: 115 RRLKTRVVKNNCNPEWNDELTLSVADLRTPIGLSVYDKDTLTDHDKMGDAEIDIEPYIEC 174
           ++LKTRVV+NNCNPEWN+ELTL++     P+ L VYDKDT T HDKMGDA+IDI+P++E 
Sbjct: 37  QKLKTRVVENNCNPEWNEELTLALRHPDEPVNLIVYDKDTFTSHDKMGDAKIDIKPFLEV 96

Query: 175 LKMGLESLPDGCVVKRVYPKRTNNLADESQCVWQKGKIVQNMFLRLKNVECGEVAIQLEW 234
            KMGL+ LPDG  +KRV P R N LA+ S  V   GKIVQNM L L+NVECGEV IQLEW
Sbjct: 97  HKMGLQELPDGTEIKRVVPNRENCLAEASSIVSNNGKIVQNMILLLRNVECGEVEIQLEW 156

Query: 235 IDVPGAKGL 244
           ID+PG++GL
Sbjct: 157 IDIPGSRGL 165

[Close]

BLAST of Lsi09G006650 vs. Swiss-Prot
Match: CAR9_ARATH (Protein C2-DOMAIN ABA-RELATED 9 OS=Arabidopsis thaliana GN=CAR9 PE=2 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 7.3e-42
Identity = 77/131 (58.78%), Postives = 97/131 (74.05%), Query Frame = 1


Query: 115 RRLKTRVVKNNCNPEWNDELTLSVADLRTPIGLSVYDKDTLTDHDKMGDAEIDIEPYIEC 174
           ++LKTRV+ NNCNP WN++LTLS+ D+  PI L+V+DKD  +  DKMGDAEID  P++E 
Sbjct: 38  QKLKTRVINNNCNPVWNEQLTLSIKDVNDPIRLTVFDKDRFSGDDKMGDAEIDFRPFLEA 97

Query: 175 LKMGLE--SLPDGCVVKRVYPKRTNNLADESQCVWQKGKIVQNMFLRLKNVECGEVAIQL 234
            +M L+   LP+GC +KR+ P RTN LA+ES   W  GKI+Q M LRLKNVECGEV + L
Sbjct: 98  HQMELDFQKLPNGCAIKRIRPGRTNCLAEESSITWSNGKIMQEMILRLKNVECGEVELML 157

Query: 235 EWIDVPGAKGL 244
           EW D PG KGL
Sbjct: 158 EWTDGPGCKGL 168

[Close]

BLAST of Lsi09G006650 vs. Swiss-Prot
Match: CAR10_ARATH (Protein C2-DOMAIN ABA-RELATED 10 OS=Arabidopsis thaliana GN=CAR10 PE=2 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 4.0e-40
Identity = 75/129 (58.14%), Postives = 94/129 (72.87%), Query Frame = 1


Query: 117 LKTRVVKNNCNPEWNDELTLSVADLRTPIGLSVYDKDTLTDHDKMGDAEIDIEPYIECLK 176
           LKTRVVK NCNP WN+E+T+++ D   PI L+V+D D  T  DKMGDA IDI+PY+E LK
Sbjct: 40  LKTRVVKKNCNPVWNEEMTVAIKDPNVPIRLTVFDWDKFTGDDKMGDANIDIQPYLEALK 99

Query: 177 MGLE--SLPDGCVVKRVYPKRTNNLADESQCVWQKGKIVQNMFLRLKNVECGEVAIQLEW 236
           MG+E   LP+GC +KRV P R N L+DES  VW  GKI Q++ LRL NVECGE+ I LEW
Sbjct: 100 MGMELLRLPNGCAIKRVQPSRHNCLSDESSIVWNNGKITQDLILRLNNVECGEIEIMLEW 159

Query: 237 IDVPGAKGL 244
            +  G +G+
Sbjct: 160 HEGAGCRGI 168

[Close]

BLAST of Lsi09G006650 vs. Swiss-Prot
Match: CAR1_ARATH (Protein C2-DOMAIN ABA-RELATED 1 OS=Arabidopsis thaliana GN=CAR1 PE=1 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 1.7e-38
Identity = 75/132 (56.82%), Postives = 100/132 (75.76%), Query Frame = 1


Query: 115 RRLKTRVVKNNCNPEWNDELTLSVADLRTPIGLSVYDKDTLTDHDKMGDAEIDIEPYIEC 174
           ++LKTRVVK++ NPEWND+LTLSV D   PI L+VYD D L+  DKMG+AE  I P+IE 
Sbjct: 37  QKLKTRVVKHSVNPEWNDDLTLSVTDPNLPIKLTVYDYDLLSADDKMGEAEFHIGPFIEA 96

Query: 175 LKMGLE---SLPDGCVVKRVYPKRTNNLADESQCVWQKGKIVQNMFLRLKNVECGEVAIQ 234
           +K   +    LP+G ++K++ P R N L++ S  V  +GKIVQNMFLRL++VECGEV +Q
Sbjct: 97  IKFAHQLGPGLPNGTIIKKIEPSRKNCLSESSHIVLNQGKIVQNMFLRLQHVECGEVELQ 156

Query: 235 LEWIDVPGAKGL 244
           LEWIDVPG++G+
Sbjct: 157 LEWIDVPGSRGI 168

[Close]

BLAST of Lsi09G006650 vs. Swiss-Prot
Match: CAR4_ARATH (Protein C2-DOMAIN ABA-RELATED 4 OS=Arabidopsis thaliana GN=CAR4 PE=1 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 1.7e-38
Identity = 72/129 (55.81%), Postives = 96/129 (74.42%), Query Frame = 1


Query: 115 RRLKTRVVKNNCNPEWNDELTLSVADLRTPIGLSVYDKDTLTDHDKMGDAEIDIEPYIEC 174
           ++LKTRV+  + NPEWN++LTLSV D    + L+VYD D  +  DKMGDAE +I+PYIE 
Sbjct: 49  QKLKTRVINKDVNPEWNEDLTLSVTDSNLTVLLTVYDHDMFSKDDKMGDAEFEIKPYIEA 108

Query: 175 LKMGLESLPDGCVVKRVYPKRTNNLADESQCVWQKGKIVQNMFLRLKNVECGEVAIQLEW 234
           L+M L+ LP G +V  V P R N LA+ES+  W  GK+VQ++ LRL++VECGEV  QL+W
Sbjct: 109 LRMQLDGLPSGTIVTTVKPSRRNCLAEESRVTWVDGKLVQDLVLRLRHVECGEVEAQLQW 168

Query: 235 IDVPGAKGL 244
           ID+PG+KGL
Sbjct: 169 IDLPGSKGL 177

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BLAST of Lsi09G006650 vs. TrEMBL
Match: A0A0A0KRJ0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G569360 PE=4 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 7.1e-68
Identity = 122/137 (89.05%), Postives = 130/137 (94.89%), Query Frame = 1


Query: 115 RRLKTRVVKNNCNPEWNDELTLSVADLRTPIGLSVYDKDTLTDHDKMGDAEIDIEPYIEC 174
           +RLKTRVVKNNCNPEWNDELTLSVADLRTPIGLSVYDKDTLTDHDKMGDAEIDI PYIEC
Sbjct: 37  QRLKTRVVKNNCNPEWNDELTLSVADLRTPIGLSVYDKDTLTDHDKMGDAEIDIGPYIEC 96

Query: 175 LKMGLESLPDGCVVKRVYPKRTNNLADESQCVWQKGKIVQNMFLRLKNVECGEVAIQLEW 234
           L+MGL+SLPDGCVV+R+YP RTN LADESQCVWQKGKIVQNM LRLKN ECGEVA+QLEW
Sbjct: 97  LRMGLQSLPDGCVVRRLYPSRTNCLADESQCVWQKGKIVQNMILRLKNAECGEVAVQLEW 156

Query: 235 IDVPGAKGLPIGGTSHF 252
           I+VPGAKGLP G TS+F
Sbjct: 157 INVPGAKGLPDGSTSNF 173

[Close]

BLAST of Lsi09G006650 vs. TrEMBL
Match: W9S150_9ROSA (Putative ADP-ribosylation factor GTPase-activating protein AGD13 OS=Morus notabilis GN=L484_000411 PE=4 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 2.1e-51
Identity = 93/130 (71.54%), Postives = 111/130 (85.38%), Query Frame = 1


Query: 114 IRRLKTRVVKNNCNPEWNDELTLSVADLRTPIGLSVYDKDTLTDHDKMGDAEIDIEPYIE 173
           +++LKTRVVKNNCNPEWNDELTLS+ DL TPI L+V+D+DTLT  DKMGDAEIDI+PYIE
Sbjct: 1   MQKLKTRVVKNNCNPEWNDELTLSITDLETPITLTVFDRDTLTSDDKMGDAEIDIKPYIE 60

Query: 174 CLKMGLESLPDGCVVKRVYPKRTNNLADESQCVWQKGKIVQNMFLRLKNVECGEVAIQLE 233
           CLKMGLE+ PDG VVKR+ P   N L+DES CVW  GKIVQ+M LRL+NVECG++ +Q+E
Sbjct: 61  CLKMGLENRPDGSVVKRIQPSHENCLSDESSCVWTNGKIVQDMCLRLRNVECGKIVVQIE 120

Query: 234 WIDVPGAKGL 244
           WI+ PG KGL
Sbjct: 121 WINFPGCKGL 130

[Close]

BLAST of Lsi09G006650 vs. TrEMBL
Match: W9QZY4_9ROSA (ADP-ribosylation factor GTPase-activating protein AGD12 OS=Morus notabilis GN=L484_002690 PE=4 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 3.5e-51
Identity = 93/128 (72.66%), Postives = 109/128 (85.16%), Query Frame = 1


Query: 116 RLKTRVVKNNCNPEWNDELTLSVADLRTPIGLSVYDKDTLTDHDKMGDAEIDIEPYIECL 175
           +LKTRVVKNNCNPEWNDELTLS+ DL TPI L+V+D+DTLT  DKMGDAEIDI+PYIECL
Sbjct: 38  KLKTRVVKNNCNPEWNDELTLSITDLETPITLTVFDRDTLTSDDKMGDAEIDIKPYIECL 97

Query: 176 KMGLESLPDGCVVKRVYPKRTNNLADESQCVWQKGKIVQNMFLRLKNVECGEVAIQLEWI 235
           KMGLE+ PDG VVKR+ P   N L+DES CVW  GKIVQ+M LRL+NVECG++ +Q+EWI
Sbjct: 98  KMGLENRPDGSVVKRIQPSHENCLSDESSCVWTNGKIVQDMCLRLRNVECGKIVVQIEWI 157

Query: 236 DVPGAKGL 244
           + PG KGL
Sbjct: 158 NFPGCKGL 165

[Close]

BLAST of Lsi09G006650 vs. TrEMBL
Match: A5BXZ0_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0011g05230 PE=4 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 4.3e-49
Identity = 91/129 (70.54%), Postives = 107/129 (82.95%), Query Frame = 1


Query: 115 RRLKTRVVKNNCNPEWNDELTLSVADLRTPIGLSVYDKDTLTDHDKMGDAEIDIEPYIEC 174
           ++LKTRVVK+NCNPEWN+ELTLS+AD   PI L VYD DT T  DKMGDAEIDI+PY+EC
Sbjct: 37  QKLKTRVVKDNCNPEWNEELTLSIADTDVPINLVVYDSDTFTLDDKMGDAEIDIKPYVEC 96

Query: 175 LKMGLESLPDGCVVKRVYPKRTNNLADESQCVWQKGKIVQNMFLRLKNVECGEVAIQLEW 234
           LKMGLE+LP G V+ RV P RTN LADES CVW  GKI Q+M LRL+NVECGEV +Q+EW
Sbjct: 97  LKMGLENLPTGTVISRVQPSRTNCLADESCCVWDNGKIRQDMLLRLRNVECGEVEVQIEW 156

Query: 235 IDVPGAKGL 244
           I++PG +GL
Sbjct: 157 INIPGCRGL 165

[Close]

BLAST of Lsi09G006650 vs. TrEMBL
Match: A0A0R0I760_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_09G117100 PE=4 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 2.8e-48
Identity = 85/129 (65.89%), Postives = 110/129 (85.27%), Query Frame = 1


Query: 115 RRLKTRVVKNNCNPEWNDELTLSVADLRTPIGLSVYDKDTLTDHDKMGDAEIDIEPYIEC 174
           ++LKTRVVKNNCNP+WN+ELTLSV D++TPI L+VYDKDT +  DKMG+AEID++PY++C
Sbjct: 47  QKLKTRVVKNNCNPDWNEELTLSVKDVKTPIHLTVYDKDTFSVDDKMGEAEIDLKPYVQC 106

Query: 175 LKMGLESLPDGCVVKRVYPKRTNNLADESQCVWQKGKIVQNMFLRLKNVECGEVAIQLEW 234
            +MGL  LP+GC +KR+ P RTN LA+ES C+WQ GKIVQ MFLRL+NVE GE+ +++EW
Sbjct: 107 KQMGLGKLPNGCSLKRIQPDRTNYLAEESSCIWQNGKIVQEMFLRLRNVESGEILVEIEW 166

Query: 235 IDVPGAKGL 244
           +DV G KGL
Sbjct: 167 VDVVGCKGL 175

[Close]

BLAST of Lsi09G006650 vs. TAIR10
Match: AT1G70810.1 (AT1G70810.1 Calcium-dependent lipid-binding (CaLB domain) family protein)

HSP 1 Score: 184.1 bits (466), Expect = 1.1e-46
Identity = 84/129 (65.12%), Postives = 102/129 (79.07%), Query Frame = 1


Query: 115 RRLKTRVVKNNCNPEWNDELTLSVADLRTPIGLSVYDKDTLTDHDKMGDAEIDIEPYIEC 174
           ++LKTRVV+NNCNPEWN+ELTL++     P+ L VYDKDT T HDKMGDA+IDI+P++E 
Sbjct: 37  QKLKTRVVENNCNPEWNEELTLALRHPDEPVNLIVYDKDTFTSHDKMGDAKIDIKPFLEV 96

Query: 175 LKMGLESLPDGCVVKRVYPKRTNNLADESQCVWQKGKIVQNMFLRLKNVECGEVAIQLEW 234
            KMGL+ LPDG  +KRV P R N LA+ S  V   GKIVQNM L L+NVECGEV IQLEW
Sbjct: 97  HKMGLQELPDGTEIKRVVPNRENCLAEASSIVSNNGKIVQNMILLLRNVECGEVEIQLEW 156

Query: 235 IDVPGAKGL 244
           ID+PG++GL
Sbjct: 157 IDIPGSRGL 165

[Close]

BLAST of Lsi09G006650 vs. TAIR10
Match: AT1G70790.2 (AT1G70790.2 Calcium-dependent lipid-binding (CaLB domain) family protein)

HSP 1 Score: 172.2 bits (435), Expect = 4.1e-43
Identity = 77/131 (58.78%), Postives = 97/131 (74.05%), Query Frame = 1


Query: 115 RRLKTRVVKNNCNPEWNDELTLSVADLRTPIGLSVYDKDTLTDHDKMGDAEIDIEPYIEC 174
           ++LKTRV+ NNCNP WN++LTLS+ D+  PI L+V+DKD  +  DKMGDAEID  P++E 
Sbjct: 38  QKLKTRVINNNCNPVWNEQLTLSIKDVNDPIRLTVFDKDRFSGDDKMGDAEIDFRPFLEA 97

Query: 175 LKMGLE--SLPDGCVVKRVYPKRTNNLADESQCVWQKGKIVQNMFLRLKNVECGEVAIQL 234
            +M L+   LP+GC +KR+ P RTN LA+ES   W  GKI+Q M LRLKNVECGEV + L
Sbjct: 98  HQMELDFQKLPNGCAIKRIRPGRTNCLAEESSITWSNGKIMQEMILRLKNVECGEVELML 157

Query: 235 EWIDVPGAKGL 244
           EW D PG KGL
Sbjct: 158 EWTDGPGCKGL 168

[Close]

BLAST of Lsi09G006650 vs. TAIR10
Match: AT2G01540.1 (AT2G01540.1 Calcium-dependent lipid-binding (CaLB domain) family protein)

HSP 1 Score: 166.4 bits (420), Expect = 2.3e-41
Identity = 75/129 (58.14%), Postives = 94/129 (72.87%), Query Frame = 1


Query: 117 LKTRVVKNNCNPEWNDELTLSVADLRTPIGLSVYDKDTLTDHDKMGDAEIDIEPYIECLK 176
           LKTRVVK NCNP WN+E+T+++ D   PI L+V+D D  T  DKMGDA IDI+PY+E LK
Sbjct: 40  LKTRVVKKNCNPVWNEEMTVAIKDPNVPIRLTVFDWDKFTGDDKMGDANIDIQPYLEALK 99

Query: 177 MGLE--SLPDGCVVKRVYPKRTNNLADESQCVWQKGKIVQNMFLRLKNVECGEVAIQLEW 236
           MG+E   LP+GC +KRV P R N L+DES  VW  GKI Q++ LRL NVECGE+ I LEW
Sbjct: 100 MGMELLRLPNGCAIKRVQPSRHNCLSDESSIVWNNGKITQDLILRLNNVECGEIEIMLEW 159

Query: 237 IDVPGAKGL 244
            +  G +G+
Sbjct: 160 HEGAGCRGI 168

[Close]

BLAST of Lsi09G006650 vs. TAIR10
Match: AT3G17980.1 (AT3G17980.1 Calcium-dependent lipid-binding (CaLB domain) family protein)

HSP 1 Score: 161.0 bits (406), Expect = 9.5e-40
Identity = 72/129 (55.81%), Postives = 96/129 (74.42%), Query Frame = 1


Query: 115 RRLKTRVVKNNCNPEWNDELTLSVADLRTPIGLSVYDKDTLTDHDKMGDAEIDIEPYIEC 174
           ++LKTRV+  + NPEWN++LTLSV D    + L+VYD D  +  DKMGDAE +I+PYIE 
Sbjct: 49  QKLKTRVINKDVNPEWNEDLTLSVTDSNLTVLLTVYDHDMFSKDDKMGDAEFEIKPYIEA 108

Query: 175 LKMGLESLPDGCVVKRVYPKRTNNLADESQCVWQKGKIVQNMFLRLKNVECGEVAIQLEW 234
           L+M L+ LP G +V  V P R N LA+ES+  W  GK+VQ++ LRL++VECGEV  QL+W
Sbjct: 109 LRMQLDGLPSGTIVTTVKPSRRNCLAEESRVTWVDGKLVQDLVLRLRHVECGEVEAQLQW 168

Query: 235 IDVPGAKGL 244
           ID+PG+KGL
Sbjct: 169 IDLPGSKGL 177

[Close]

BLAST of Lsi09G006650 vs. TAIR10
Match: AT5G37740.2 (AT5G37740.2 Calcium-dependent lipid-binding (CaLB domain) family protein)

HSP 1 Score: 160.6 bits (405), Expect = 1.2e-39
Identity = 75/131 (57.25%), Postives = 99/131 (75.57%), Query Frame = 1


Query: 116 RLKTRVVKNNCNPEWNDELTLSVADLRTPIGLSVYDKDTLTDHDKMGDAEIDIEPYIECL 175
           +LKTRVVK++ NPEWND+LTLSV D   PI L+VYD D L+  DKMG+AE  I P+IE +
Sbjct: 48  KLKTRVVKHSVNPEWNDDLTLSVTDPNLPIKLTVYDYDLLSADDKMGEAEFHIGPFIEAI 107

Query: 176 KMGLE---SLPDGCVVKRVYPKRTNNLADESQCVWQKGKIVQNMFLRLKNVECGEVAIQL 235
           K   +    LP+G ++K++ P R N L++ S  V  +GKIVQNMFLRL++VECGEV +QL
Sbjct: 108 KFAHQLGPGLPNGTIIKKIEPSRKNCLSESSHIVLNQGKIVQNMFLRLQHVECGEVELQL 167

Query: 236 EWIDVPGAKGL 244
           EWIDVPG++G+
Sbjct: 168 EWIDVPGSRGI 178

[Close]

BLAST of Lsi09G006650 vs. NCBI nr
Match: gi|659072854|ref|XP_008467129.1| (PREDICTED: probable ADP-ribosylation factor GTPase-activating protein AGD11 [Cucumis melo])

HSP 1 Score: 266.5 bits (680), Expect = 4.6e-68
Identity = 122/137 (89.05%), Postives = 129/137 (94.16%), Query Frame = 1


Query: 115 RRLKTRVVKNNCNPEWNDELTLSVADLRTPIGLSVYDKDTLTDHDKMGDAEIDIEPYIEC 174
           ++LKTRVVKNNCNPEWNDELTLSVADLRTPIG+SVYDKDTLTDHDKMGDAEIDIEPYIEC
Sbjct: 37  QKLKTRVVKNNCNPEWNDELTLSVADLRTPIGISVYDKDTLTDHDKMGDAEIDIEPYIEC 96

Query: 175 LKMGLESLPDGCVVKRVYPKRTNNLADESQCVWQKGKIVQNMFLRLKNVECGEVAIQLEW 234
           LKMGL+SLPDGCVV+R+YP  TN LADESQCVW  GKIVQNM LRLKNVECGEVAIQLEW
Sbjct: 97  LKMGLQSLPDGCVVRRLYPSGTNYLADESQCVWHNGKIVQNMILRLKNVECGEVAIQLEW 156

Query: 235 IDVPGAKGLPIGGTSHF 252
           IDVPGAKGLP+G TS F
Sbjct: 157 IDVPGAKGLPVGSTSDF 173

[Close]

BLAST of Lsi09G006650 vs. NCBI nr
Match: gi|449465757|ref|XP_004150594.1| (PREDICTED: probable ADP-ribosylation factor GTPase-activating protein AGD11 [Cucumis sativus])

HSP 1 Score: 265.4 bits (677), Expect = 1.0e-67
Identity = 122/137 (89.05%), Postives = 130/137 (94.89%), Query Frame = 1


Query: 115 RRLKTRVVKNNCNPEWNDELTLSVADLRTPIGLSVYDKDTLTDHDKMGDAEIDIEPYIEC 174
           +RLKTRVVKNNCNPEWNDELTLSVADLRTPIGLSVYDKDTLTDHDKMGDAEIDI PYIEC
Sbjct: 37  QRLKTRVVKNNCNPEWNDELTLSVADLRTPIGLSVYDKDTLTDHDKMGDAEIDIGPYIEC 96

Query: 175 LKMGLESLPDGCVVKRVYPKRTNNLADESQCVWQKGKIVQNMFLRLKNVECGEVAIQLEW 234
           L+MGL+SLPDGCVV+R+YP RTN LADESQCVWQKGKIVQNM LRLKN ECGEVA+QLEW
Sbjct: 97  LRMGLQSLPDGCVVRRLYPSRTNCLADESQCVWQKGKIVQNMILRLKNAECGEVAVQLEW 156

Query: 235 IDVPGAKGLPIGGTSHF 252
           I+VPGAKGLP G TS+F
Sbjct: 157 INVPGAKGLPDGSTSNF 173

[Close]

BLAST of Lsi09G006650 vs. NCBI nr
Match: gi|1009154994|ref|XP_015895476.1| (PREDICTED: protein C2-DOMAIN ABA-RELATED 7-like [Ziziphus jujuba])

HSP 1 Score: 221.1 bits (562), Expect = 2.2e-54
Identity = 101/131 (77.10%), Postives = 114/131 (87.02%), Query Frame = 1


Query: 115 RRLKTRVVKNNCNPEWNDELTLSVADLRTPIGLSVYDKDTLTDHDKMGDAEIDIEPYIEC 174
           +RLK+RV+K NCNPEWNDELTLS++DL TPI L+VYDKDTLT  DKMG+AEIDI+P IEC
Sbjct: 37  QRLKSRVIKKNCNPEWNDELTLSISDLDTPITLTVYDKDTLTVDDKMGEAEIDIKPLIEC 96

Query: 175 LKMGLESLPDGCVVKRVYPKRTNNLADESQCVWQKGKIVQNMFLRLKNVECGEVAIQLEW 234
           L+MGLE LP+GCVVKRV P RTN LADES CVW  GKIVQNM LRLKNVECGEV +QLEW
Sbjct: 97  LRMGLEKLPNGCVVKRVQPNRTNCLADESSCVWNNGKIVQNMSLRLKNVECGEVVLQLEW 156

Query: 235 IDVPGAKGLPI 246
           ID+PG KGL +
Sbjct: 157 IDLPGCKGLGV 167

[Close]

BLAST of Lsi09G006650 vs. NCBI nr
Match: gi|703132316|ref|XP_010105094.1| (putative ADP-ribosylation factor GTPase-activating protein AGD13 [Morus notabilis])

HSP 1 Score: 210.7 bits (535), Expect = 3.0e-51
Identity = 93/130 (71.54%), Postives = 111/130 (85.38%), Query Frame = 1


Query: 114 IRRLKTRVVKNNCNPEWNDELTLSVADLRTPIGLSVYDKDTLTDHDKMGDAEIDIEPYIE 173
           +++LKTRVVKNNCNPEWNDELTLS+ DL TPI L+V+D+DTLT  DKMGDAEIDI+PYIE
Sbjct: 1   MQKLKTRVVKNNCNPEWNDELTLSITDLETPITLTVFDRDTLTSDDKMGDAEIDIKPYIE 60

Query: 174 CLKMGLESLPDGCVVKRVYPKRTNNLADESQCVWQKGKIVQNMFLRLKNVECGEVAIQLE 233
           CLKMGLE+ PDG VVKR+ P   N L+DES CVW  GKIVQ+M LRL+NVECG++ +Q+E
Sbjct: 61  CLKMGLENRPDGSVVKRIQPSHENCLSDESSCVWTNGKIVQDMCLRLRNVECGKIVVQIE 120

Query: 234 WIDVPGAKGL 244
           WI+ PG KGL
Sbjct: 121 WINFPGCKGL 130

[Close]

BLAST of Lsi09G006650 vs. NCBI nr
Match: gi|703096053|ref|XP_010095718.1| (ADP-ribosylation factor GTPase-activating protein AGD12 [Morus notabilis])

HSP 1 Score: 209.9 bits (533), Expect = 5.1e-51
Identity = 93/128 (72.66%), Postives = 109/128 (85.16%), Query Frame = 1


Query: 116 RLKTRVVKNNCNPEWNDELTLSVADLRTPIGLSVYDKDTLTDHDKMGDAEIDIEPYIECL 175
           +LKTRVVKNNCNPEWNDELTLS+ DL TPI L+V+D+DTLT  DKMGDAEIDI+PYIECL
Sbjct: 38  KLKTRVVKNNCNPEWNDELTLSITDLETPITLTVFDRDTLTSDDKMGDAEIDIKPYIECL 97

Query: 176 KMGLESLPDGCVVKRVYPKRTNNLADESQCVWQKGKIVQNMFLRLKNVECGEVAIQLEWI 235
           KMGLE+ PDG VVKR+ P   N L+DES CVW  GKIVQ+M LRL+NVECG++ +Q+EWI
Sbjct: 98  KMGLENRPDGSVVKRIQPSHENCLSDESSCVWTNGKIVQDMCLRLRNVECGKIVVQIEWI 157

Query: 236 DVPGAKGL 244
           + PG KGL
Sbjct: 158 NFPGCKGL 165

The following BLAST results are available for this feature:

BLAST of Lsi09G006650 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CAR7_ARATH	1.9e-45	65.12	Protein C2-DOMAIN ABA-RELATED 7 OS=Arabidopsis thaliana GN=CAR7 PE=1 SV=1	[more]
CAR9_ARATH	7.3e-42	58.78	Protein C2-DOMAIN ABA-RELATED 9 OS=Arabidopsis thaliana GN=CAR9 PE=2 SV=1	[more]
CAR10_ARATH	4.0e-40	58.14	Protein C2-DOMAIN ABA-RELATED 10 OS=Arabidopsis thaliana GN=CAR10 PE=2 SV=1	[more]
CAR1_ARATH	1.7e-38	56.82	Protein C2-DOMAIN ABA-RELATED 1 OS=Arabidopsis thaliana GN=CAR1 PE=1 SV=1	[more]
CAR4_ARATH	1.7e-38	55.81	Protein C2-DOMAIN ABA-RELATED 4 OS=Arabidopsis thaliana GN=CAR4 PE=1 SV=1	[more]

BLAST of Lsi09G006650 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KRJ0_CUCSA	7.1e-68	89.05	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G569360 PE=4 SV=1	[more]
W9S150_9ROSA	2.1e-51	71.54	Putative ADP-ribosylation factor GTPase-activating protein AGD13 OS=Morus notabi...	[more]
W9QZY4_9ROSA	3.5e-51	72.66	ADP-ribosylation factor GTPase-activating protein AGD12 OS=Morus notabilis GN=L4...	[more]
A5BXZ0_VITVI	4.3e-49	70.54	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0011g05230 PE=4 SV=...	[more]
A0A0R0I760_SOYBN	2.8e-48	65.89	Uncharacterized protein OS=Glycine max GN=GLYMA_09G117100 PE=4 SV=1	[more]

BLAST of Lsi09G006650 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G70810.1	1.1e-46	65.12	Calcium-dependent lipid-binding (CaLB domain) family protein	[more]
AT1G70790.2	4.1e-43	58.78	Calcium-dependent lipid-binding (CaLB domain) family protein	[more]
AT2G01540.1	2.3e-41	58.14	Calcium-dependent lipid-binding (CaLB domain) family protein	[more]
AT3G17980.1	9.5e-40	55.81	Calcium-dependent lipid-binding (CaLB domain) family protein	[more]
AT5G37740.2	1.2e-39	57.25	Calcium-dependent lipid-binding (CaLB domain) family protein	[more]

BLAST of Lsi09G006650 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659072854\|ref\|XP_008467129.1\|	4.6e-68	89.05	PREDICTED: probable ADP-ribosylation factor GTPase-activating protein AGD11 [Cuc...	[more]
gi\|449465757\|ref\|XP_004150594.1\|	1.0e-67	89.05	PREDICTED: probable ADP-ribosylation factor GTPase-activating protein AGD11 [Cuc...	[more]
gi\|1009154994\|ref\|XP_015895476.1\|	2.2e-54	77.10	PREDICTED: protein C2-DOMAIN ABA-RELATED 7-like [Ziziphus jujuba]	[more]
gi\|703132316\|ref\|XP_010105094.1\|	3.0e-51	71.54	putative ADP-ribosylation factor GTPase-activating protein AGD13 [Morus notabili...	[more]
gi\|703096053\|ref\|XP_010095718.1\|	5.1e-51	72.66	ADP-ribosylation factor GTPase-activating protein AGD12 [Morus notabilis]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005515	protein binding

Vocabulary: **INTERPRO**
Term	Definition
IPR000008	C2_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046486	glycerolipid metabolic process
biological_process	GO:0006544	glycine metabolic process
biological_process	GO:0006563	L-serine metabolic process
biological_process	GO:0006566	threonine metabolic process
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004609	phosphatidylserine decarboxylase activity
molecular_function	GO:0005515	protein binding
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi09G006650.1	Lsi09G006650.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000008	C2 domain	GENE3D	G3DSA:2.60.40.150		coord: 6..37 score: 1.2E-20coord: 114..173 score: 1.2
IPR000008	C2 domain	PFAM	PF00168	C2	coord: 6..37 score: 0.019coord: 115..170 score: 6.6
IPR000008	C2 domain	SMART	SM00239	C2_3c	coord: 7..180 score: 2.9
IPR000008	C2 domain	PROFILE	PS50004	C2	coord: 115..165 score: 9
IPR000008	C2 domain	unknown	SSF49562	C2 domain (Calcium/lipid-binding domain, CaLB)	coord: 204..234 score: 3.77E-19coord: 111..169 score: 3.77E-19coord: 3..35 score: 3.77
None	No IPR available	PANTHER	PTHR23180	CENTAURIN/ARF	coord: 116..238 score: 1.7E-70coord: 1..37 score: 1.7
None	No IPR available	PANTHER	PTHR23180:SF268	CALCIUM-DEPENDENT LIPID-BINDING DOMAIN-CONTAINING PROTEIN	coord: 116..238 score: 1.7E-70coord: 1..37 score: 1.7

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi09G006650	Csa5G569360	Cucumber (Chinese Long) v2	culsiB329
Lsi09G006650	CSPI05G19520	Wild cucumber (PI 183967)	cpilsiB329
Lsi09G006650	Cucsa.280320	Cucumber (Gy14) v1	cgylsiB452
Lsi09G006650	CmaCh04G021220	Cucurbita maxima (Rimu)	cmalsiB626
Lsi09G006650	CmoCh04G022210	Cucurbita moschata (Rifu)	cmolsiB615
Lsi09G006650	Cla003931	Watermelon (97103) v1	lsiwmB028
Lsi09G006650	MELO3C005299	Melon (DHL92) v3.5.1	lsimeB013
Lsi09G006650	ClCG01G006300	Watermelon (Charleston Gray)	lsiwcgB008
Lsi09G006650	MELO3C005299.2	Melon (DHL92) v3.6.1	lsimedB013
Lsi09G006650	Cla97C01G006610	Watermelon (97103) v2	lsiwmbB007
Lsi09G006650	Bhi12G000789	Wax gourd	lsiwgoB006
Lsi09G006650	Cp4.1LG01g18510	Cucurbita pepo (Zucchini)	cpelsiB315
Lsi09G006650	CsGy5G019030	Cucumber (Gy14) v2	cgyblsiB301
Lsi09G006650	Carg25238	Silver-seed gourd	carlsiB800
Lsi09G006650	CsaV3_5G028360	Cucumber (Chinese Long) v3	cuclsiB352

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi09G006650	Silver-seed gourd	carlsiB169
Lsi09G006650	Silver-seed gourd	carlsiB387
Lsi09G006650	Cucurbita maxima (Rimu)	cmalsiB263
Lsi09G006650	Cucurbita maxima (Rimu)	cmalsiB622
Lsi09G006650	Cucurbita moschata (Rifu)	cmolsiB250
Lsi09G006650	Cucurbita moschata (Rifu)	cmolsiB610
Lsi09G006650	Cucurbita pepo (Zucchini)	cpelsiB135
Lsi09G006650	Cucurbita pepo (Zucchini)	cpelsiB311