Lsi09G005750 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi09G005750
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Serine/Threonine kinase family protein
Location	chr09 : 6190954 .. 6191825 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGAAGGAGATTTTGTTGTTTCCTAGAACAATTGTGAAGGAGTTCTCTCTCCTCGAAACGGATGCTCCCCCTCTTCCGGTTACCCCCACCCTGCCTTCCCCCATGCTTTCGACCATGATCTGTCAAGCTCCAAAGCTCAAGAGAAGTGGAAATGATGTTGGAGTCTTTTATCTGTGAAGATTGAATACTATGTTTTTGTGTGCAGTTTCGATAGAGAGAAAAGGTAGAGTTTGCAAAATCATAGAAAAGCATGGGAAAAGATTTTCCGCTCATTTGTAAGGAGGGACTTTATGGTTGATTGATGCCATTGACCAAACTTCTCATGAAATGGACATTTCATAAAACTGTGGCTTCAGCTTGTTTTTGCTTTTTCATTGAACACAGAAAGCTAGAGTTTCTATGTCCATCTTATTTTCTTTTATTGAACTTTATACTATAATAGTATATTTTTGCAGCAGCTTGAAAGAGTATTTTCTTTTGTCTCACCCAAGCTTGCATTTCACCCTTGTGACTAGGGTCAAGAACTCAAGTTCCCTTAGGCATATACCCTTTACTCATTTAGAAATATATTGTTTTTCATCCTTATACTACATACAATCGAATGGATTATGTTTTTGATTAGGTTAATTGGTTATTTCAGATATGTTGTCAAAGATCTACTTGGTCATGGGACTTTTGGGCAGGTTGCTAAATGCTGGGTTGCGGAAACTAACAGCTTTGTAGCTGTGAAGATCATAAAAAACCAACCTGCATATTATCAGCAAGCACTGGTTGAAGTATCTATTTTATCATTGGTAACTTGTTTCCATTTTCATCTATTTTGATAATGGCTATTTCCTTTCCTTTTTCTTGCTATTTAGATAATTTAG

mRNA sequence

ATGATGAAGGAGATTTTGTTGTTTCCTAGAACAATTGTGAAGGAGTTCTCTCTCCTCGAAACGGATGCTCCCCCTCTTCCGGTTACCCCCACCCTGCCTTCCCCCATGCTTTCGACCATGATCTGTCAAGCTCCAAAGCTCAAGAGAAGTGGAAATGATAAAGCTAGAGTTTCTATGTCCATCTTATTTTCTTTTATTGAACTTTATACTATAATAGTATATTTTTGCAGCAGCTTGAAAGAATATGTTGTCAAAGATCTACTTGGTCATGGGACTTTTGGGCAGGTTGCTAAATGCTGGGTTGCGGAAACTAACAGCTTTGTAGCTGTGAAGATCATAAAAAACCAACCTGCATATTATCAGCAAGCACTGGTTGAAATAATTTAG

Coding sequence (CDS)

ATGATGAAGGAGATTTTGTTGTTTCCTAGAACAATTGTGAAGGAGTTCTCTCTCCTCGAAACGGATGCTCCCCCTCTTCCGGTTACCCCCACCCTGCCTTCCCCCATGCTTTCGACCATGATCTGTCAAGCTCCAAAGCTCAAGAGAAGTGGAAATGATAAAGCTAGAGTTTCTATGTCCATCTTATTTTCTTTTATTGAACTTTATACTATAATAGTATATTTTTGCAGCAGCTTGAAAGAATATGTTGTCAAAGATCTACTTGGTCATGGGACTTTTGGGCAGGTTGCTAAATGCTGGGTTGCGGAAACTAACAGCTTTGTAGCTGTGAAGATCATAAAAAACCAACCTGCATATTATCAGCAAGCACTGGTTGAAATAATTTAG

Protein sequence

MMKEILLFPRTIVKEFSLLETDAPPLPVTPTLPSPMLSTMICQAPKLKRSGNDKARVSMSILFSFIELYTIIVYFCSSLKEYVVKDLLGHGTFGQVAKCWVAETNSFVAVKIIKNQPAYYQQALVEII

Homology

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BLAST of Lsi09G005750 vs. Swiss-Prot
Match: HIPK3_HUMAN (Homeodomain-interacting protein kinase 3 OS=Homo sapiens GN=HIPK3 PE=1 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 6.4e-10
Identity = 28/52 (53.85%), Postives = 34/52 (65.38%), Query Frame = 1


Query: 76  CSSLKEYVVKDLLGHGTFGQVAKCWVAETNSFVAVKIIKNQPAYYQQALVEI 128
           CS    Y V D LG GTFGQV KCW   TN  VA+KI+KN P+Y +Q  +E+
Sbjct: 191 CSMKNTYEVLDFLGRGTFGQVVKCWKRGTNEIVAIKILKNHPSYARQGQIEV 242

[Close]

BLAST of Lsi09G005750 vs. Swiss-Prot
Match: HIPK3_MOUSE (Homeodomain-interacting protein kinase 3 OS=Mus musculus GN=Hipk3 PE=1 SV=3)

HSP 1 Score: 65.1 bits (157), Expect = 6.4e-10
Identity = 28/52 (53.85%), Postives = 34/52 (65.38%), Query Frame = 1


Query: 76  CSSLKEYVVKDLLGHGTFGQVAKCWVAETNSFVAVKIIKNQPAYYQQALVEI 128
           CS    Y V D LG GTFGQV KCW   TN  VA+KI+KN P+Y +Q  +E+
Sbjct: 191 CSMKNTYEVLDFLGRGTFGQVVKCWKRGTNEIVAIKILKNHPSYARQGQIEV 242

[Close]

BLAST of Lsi09G005750 vs. Swiss-Prot
Match: HIPK3_RAT (Homeodomain-interacting protein kinase 3 OS=Rattus norvegicus GN=Hipk3 PE=1 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 8.4e-10
Identity = 28/52 (53.85%), Postives = 34/52 (65.38%), Query Frame = 1


Query: 76  CSSLKEYVVKDLLGHGTFGQVAKCWVAETNSFVAVKIIKNQPAYYQQALVEI 128
           CS    Y V D LG GTFGQV KCW   TN  VA+KI+KN P+Y +Q  +E+
Sbjct: 191 CSVKNTYEVLDFLGRGTFGQVVKCWKRGTNEIVAIKILKNHPSYARQGQIEV 242

[Close]

BLAST of Lsi09G005750 vs. Swiss-Prot
Match: HIPK1_RAT (Homeodomain-interacting protein kinase 1 OS=Rattus norvegicus GN=Hipk1 PE=2 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 1.1e-09
Identity = 27/52 (51.92%), Postives = 34/52 (65.38%), Query Frame = 1


Query: 76  CSSLKEYVVKDLLGHGTFGQVAKCWVAETNSFVAVKIIKNQPAYYQQALVEI 128
           CS    Y V + LG GTFGQVAKCW   T   VA+KI+KN P+Y +Q  +E+
Sbjct: 184 CSMTNSYEVLEFLGRGTFGQVAKCWKRSTKEIVAIKILKNHPSYARQGQIEV 235

[Close]

BLAST of Lsi09G005750 vs. Swiss-Prot
Match: HIPK1_MOUSE (Homeodomain-interacting protein kinase 1 OS=Mus musculus GN=Hipk1 PE=1 SV=2)

HSP 1 Score: 64.3 bits (155), Expect = 1.1e-09
Identity = 27/52 (51.92%), Postives = 34/52 (65.38%), Query Frame = 1


Query: 76  CSSLKEYVVKDLLGHGTFGQVAKCWVAETNSFVAVKIIKNQPAYYQQALVEI 128
           CS    Y V + LG GTFGQVAKCW   T   VA+KI+KN P+Y +Q  +E+
Sbjct: 184 CSMTNSYEVLEFLGRGTFGQVAKCWKRSTKEIVAIKILKNHPSYARQGQIEV 235

[Close]

BLAST of Lsi09G005750 vs. TrEMBL
Match: V4KPI2_EUTSA (Uncharacterized protein OS=Eutrema salsugineum GN=EUTSA_v10003604mg PE=3 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 1.3e-17
Identity = 46/55 (83.64%), Postives = 50/55 (90.91%), Query Frame = 1


Query: 75  FCSS--LKEYVVKDLLGHGTFGQVAKCWVAETNSFVAVKIIKNQPAYYQQALVEI 128
           FCSS   + Y+VKDLLGHGTFGQVAKCWV ETNSFVAVK+IKNQPAYYQQALVE+
Sbjct: 112 FCSSDSRQRYIVKDLLGHGTFGQVAKCWVPETNSFVAVKVIKNQPAYYQQALVEV 166

[Close]

BLAST of Lsi09G005750 vs. TrEMBL
Match: A0A087G2T6_ARAAL (Uncharacterized protein OS=Arabis alpina GN=AALP_AAs68745U000800 PE=3 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 1.3e-17
Identity = 46/55 (83.64%), Postives = 50/55 (90.91%), Query Frame = 1


Query: 75  FCSS--LKEYVVKDLLGHGTFGQVAKCWVAETNSFVAVKIIKNQPAYYQQALVEI 128
           FCSS   + Y+VKDLLGHGTFGQVAKCWV ETNSFVAVK+IKNQPAYYQQALVE+
Sbjct: 112 FCSSDSRQRYIVKDLLGHGTFGQVAKCWVPETNSFVAVKVIKNQPAYYQQALVEV 166

[Close]

BLAST of Lsi09G005750 vs. TrEMBL
Match: V4L0F9_EUTSA (Uncharacterized protein OS=Eutrema salsugineum GN=EUTSA_v10003604mg PE=3 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 1.3e-17
Identity = 46/55 (83.64%), Postives = 50/55 (90.91%), Query Frame = 1


Query: 75  FCSS--LKEYVVKDLLGHGTFGQVAKCWVAETNSFVAVKIIKNQPAYYQQALVEI 128
           FCSS   + Y+VKDLLGHGTFGQVAKCWV ETNSFVAVK+IKNQPAYYQQALVE+
Sbjct: 112 FCSSDSRQRYIVKDLLGHGTFGQVAKCWVPETNSFVAVKVIKNQPAYYQQALVEV 166

[Close]

BLAST of Lsi09G005750 vs. TrEMBL
Match: B9I6T2_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0013s07280g PE=3 SV=2)

HSP 1 Score: 96.3 bits (238), Expect = 2.9e-17
Identity = 43/48 (89.58%), Postives = 47/48 (97.92%), Query Frame = 1


Query: 80  KEYVVKDLLGHGTFGQVAKCWVAETNSFVAVKIIKNQPAYYQQALVEI 128
           + Y+VKD+LGHGTFGQVAKCWVAETNSFVAVKIIKNQPAYYQQALVE+
Sbjct: 137 RRYIVKDVLGHGTFGQVAKCWVAETNSFVAVKIIKNQPAYYQQALVEV 184

[Close]

BLAST of Lsi09G005750 vs. TrEMBL
Match: M5X6B0_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa001091mg PE=3 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 2.9e-17
Identity = 49/76 (64.47%), Postives = 58/76 (76.32%), Query Frame = 1


Query: 52  NDKARVSMSILFSFIELYTIIVYFCSSLKEYVVKDLLGHGTFGQVAKCWVAETNSFVAVK 111
           N  + + +++ F  + L T    F  +L  YVVKD+LGHGTFGQVAKCWV ETNSFVAVK
Sbjct: 48  NVNSDLILTVNFVLVNLDTQRRAFPLALFRYVVKDVLGHGTFGQVAKCWVPETNSFVAVK 107

Query: 112 IIKNQPAYYQQALVEI 128
           IIKNQPAYYQQALVE+
Sbjct: 108 IIKNQPAYYQQALVEV 123

[Close]

BLAST of Lsi09G005750 vs. TAIR10
Match: AT5G35980.1 (AT5G35980.1 yeast YAK1-related gene 1)

HSP 1 Score: 93.6 bits (231), Expect = 9.5e-20
Identity = 45/55 (81.82%), Postives = 49/55 (89.09%), Query Frame = 1


Query: 75  FCSS--LKEYVVKDLLGHGTFGQVAKCWVAETNSFVAVKIIKNQPAYYQQALVEI 128
           FCSS   + Y+VKDLLGHGTFGQVAKCWV ETNSFVAVK+IKNQ AYYQQALVE+
Sbjct: 113 FCSSDSRQRYIVKDLLGHGTFGQVAKCWVPETNSFVAVKVIKNQLAYYQQALVEV 167

[Close]

BLAST of Lsi09G005750 vs. NCBI nr
Match: gi|567143570|ref|XP_006395923.1| (hypothetical protein EUTSA_v10003604mg [Eutrema salsugineum])

HSP 1 Score: 97.4 bits (241), Expect = 1.9e-17
Identity = 46/55 (83.64%), Postives = 50/55 (90.91%), Query Frame = 1


Query: 75  FCSS--LKEYVVKDLLGHGTFGQVAKCWVAETNSFVAVKIIKNQPAYYQQALVEI 128
           FCSS   + Y+VKDLLGHGTFGQVAKCWV ETNSFVAVK+IKNQPAYYQQALVE+
Sbjct: 112 FCSSDSRQRYIVKDLLGHGTFGQVAKCWVPETNSFVAVKVIKNQPAYYQQALVEV 166

[Close]

BLAST of Lsi09G005750 vs. NCBI nr
Match: gi|727532080|ref|XP_010440780.1| (PREDICTED: serine/threonine-protein kinase ppk15-like isoform X2 [Camelina sativa])

HSP 1 Score: 97.4 bits (241), Expect = 1.9e-17
Identity = 46/55 (83.64%), Postives = 50/55 (90.91%), Query Frame = 1


Query: 75  FCSS--LKEYVVKDLLGHGTFGQVAKCWVAETNSFVAVKIIKNQPAYYQQALVEI 128
           FCSS   + Y+VKDLLGHGTFGQVAKCWV ETNSFVAVK+IKNQPAYYQQALVE+
Sbjct: 105 FCSSDSRQRYIVKDLLGHGTFGQVAKCWVPETNSFVAVKVIKNQPAYYQQALVEV 159

[Close]

BLAST of Lsi09G005750 vs. NCBI nr
Match: gi|674230403|gb|KFK24188.1| (hypothetical protein AALP_AAs68745U000800 [Arabis alpina])

HSP 1 Score: 97.4 bits (241), Expect = 1.9e-17
Identity = 46/55 (83.64%), Postives = 50/55 (90.91%), Query Frame = 1


Query: 75  FCSS--LKEYVVKDLLGHGTFGQVAKCWVAETNSFVAVKIIKNQPAYYQQALVEI 128
           FCSS   + Y+VKDLLGHGTFGQVAKCWV ETNSFVAVK+IKNQPAYYQQALVE+
Sbjct: 112 FCSSDSRQRYIVKDLLGHGTFGQVAKCWVPETNSFVAVKVIKNQPAYYQQALVEV 166

[Close]

BLAST of Lsi09G005750 vs. NCBI nr
Match: gi|567143565|ref|XP_006395922.1| (hypothetical protein EUTSA_v10003604mg [Eutrema salsugineum])

HSP 1 Score: 97.4 bits (241), Expect = 1.9e-17
Identity = 46/55 (83.64%), Postives = 50/55 (90.91%), Query Frame = 1


Query: 75  FCSS--LKEYVVKDLLGHGTFGQVAKCWVAETNSFVAVKIIKNQPAYYQQALVEI 128
           FCSS   + Y+VKDLLGHGTFGQVAKCWV ETNSFVAVK+IKNQPAYYQQALVE+
Sbjct: 112 FCSSDSRQRYIVKDLLGHGTFGQVAKCWVPETNSFVAVKVIKNQPAYYQQALVEV 166

[Close]

BLAST of Lsi09G005750 vs. NCBI nr
Match: gi|727651778|ref|XP_010496604.1| (PREDICTED: uncharacterized protein LOC104773658 [Camelina sativa])

HSP 1 Score: 97.4 bits (241), Expect = 1.9e-17
Identity = 46/55 (83.64%), Postives = 50/55 (90.91%), Query Frame = 1


Query: 75  FCSS--LKEYVVKDLLGHGTFGQVAKCWVAETNSFVAVKIIKNQPAYYQQALVEI 128
           FCSS   + Y+VKDLLGHGTFGQVAKCWV ETNSFVAVK+IKNQPAYYQQALVE+
Sbjct: 105 FCSSDSRQRYIVKDLLGHGTFGQVAKCWVPETNSFVAVKVIKNQPAYYQQALVEV 159

The following BLAST results are available for this feature:

BLAST of Lsi09G005750 vs. Swiss-Prot


Match Name	E-value	Identity	Description
HIPK3_HUMAN	6.4e-10	53.85	Homeodomain-interacting protein kinase 3 OS=Homo sapiens GN=HIPK3 PE=1 SV=1	[more]
HIPK3_MOUSE	6.4e-10	53.85	Homeodomain-interacting protein kinase 3 OS=Mus musculus GN=Hipk3 PE=1 SV=3	[more]
HIPK3_RAT	8.4e-10	53.85	Homeodomain-interacting protein kinase 3 OS=Rattus norvegicus GN=Hipk3 PE=1 SV=1	[more]
HIPK1_RAT	1.1e-09	51.92	Homeodomain-interacting protein kinase 1 OS=Rattus norvegicus GN=Hipk1 PE=2 SV=1	[more]
HIPK1_MOUSE	1.1e-09	51.92	Homeodomain-interacting protein kinase 1 OS=Mus musculus GN=Hipk1 PE=1 SV=2	[more]

BLAST of Lsi09G005750 vs. TrEMBL


Match Name	E-value	Identity	Description
V4KPI2_EUTSA	1.3e-17	83.64	Uncharacterized protein OS=Eutrema salsugineum GN=EUTSA_v10003604mg PE=3 SV=1	[more]
A0A087G2T6_ARAAL	1.3e-17	83.64	Uncharacterized protein OS=Arabis alpina GN=AALP_AAs68745U000800 PE=3 SV=1	[more]
V4L0F9_EUTSA	1.3e-17	83.64	Uncharacterized protein OS=Eutrema salsugineum GN=EUTSA_v10003604mg PE=3 SV=1	[more]
B9I6T2_POPTR	2.9e-17	89.58	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0013s07280g PE=3 SV=2	[more]
M5X6B0_PRUPE	2.9e-17	64.47	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa001091mg PE=3 SV=1	[more]

BLAST of Lsi09G005750 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G35980.1	9.5e-20	81.82	yeast YAK1-related gene 1	[more]

BLAST of Lsi09G005750 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|567143570\|ref\|XP_006395923.1\|	1.9e-17	83.64	hypothetical protein EUTSA_v10003604mg [Eutrema salsugineum]	[more]
gi\|727532080\|ref\|XP_010440780.1\|	1.9e-17	83.64	PREDICTED: serine/threonine-protein kinase ppk15-like isoform X2 [Camelina sativ...	[more]
gi\|674230403\|gb\|KFK24188.1\|	1.9e-17	83.64	hypothetical protein AALP_AAs68745U000800 [Arabis alpina]	[more]
gi\|567143565\|ref\|XP_006395922.1\|	1.9e-17	83.64	hypothetical protein EUTSA_v10003604mg [Eutrema salsugineum]	[more]
gi\|727651778\|ref\|XP_010496604.1\|	1.9e-17	83.64	PREDICTED: uncharacterized protein LOC104773658 [Camelina sativa]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0006468	protein phosphorylation

Vocabulary: **Molecular Function**
Term	Definition
GO:0005524	ATP binding
GO:0004672	protein kinase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR017441	Protein_kinase_ATP_BS
IPR011009	Kinase-like_dom_sf
IPR000719	Prot_kinase_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
biological_process	GO:0009069	serine family amino acid metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004674	protein serine/threonine kinase activity
molecular_function	GO:0004672	protein kinase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi09G005750.1	Lsi09G005750.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	PROFILE	PS50011	PROTEIN_KINASE_DOM	coord: 82..128 score: 8
IPR011009	Protein kinase-like domain	unknown	SSF56112	Protein kinase-like (PK-like)	coord: 57..127 score: 1.59
IPR017441	Protein kinase, ATP binding site	PROSITE	PS00107	PROTEIN_KINASE_ATP	coord: 88..111 scor
None	No IPR available	GENE3D	G3DSA:3.30.200.20		coord: 79..127 score: 2.4
None	No IPR available	PANTHER	PTHR24058	DUAL SPECIFICITY PROTEIN KINASE	coord: 58..127 score: 1.3
None	No IPR available	PANTHER	PTHR24058:SF64	SUBFAMILY NOT NAMED	coord: 58..127 score: 1.3

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi09G005750	Silver-seed gourd	carlsiB648
Lsi09G005750	Silver-seed gourd	carlsiB676
Lsi09G005750	Cucumber (Chinese Long) v3	cuclsiB345
Lsi09G005750	Watermelon (97103) v2	lsiwmbB007
Lsi09G005750	Wax gourd	lsiwgoB029
Lsi09G005750	Cucumber (Gy14) v1	cgylsiB162
Lsi09G005750	Cucurbita maxima (Rimu)	cmalsiB261
Lsi09G005750	Cucurbita maxima (Rimu)	cmalsiB531
Lsi09G005750	Cucurbita moschata (Rifu)	cmolsiB248
Lsi09G005750	Cucurbita moschata (Rifu)	cmolsiB517
Lsi09G005750	Cucurbita pepo (Zucchini)	cpelsiB133
Lsi09G005750	Cucurbita pepo (Zucchini)	cpelsiB583
Lsi09G005750	Wild cucumber (PI 183967)	cpilsiB322
Lsi09G005750	Cucumber (Chinese Long) v2	culsiB322
Lsi09G005750	Melon (DHL92) v3.5.1	lsimeB013
Lsi09G005750	Watermelon (Charleston Gray)	lsiwcgB008
Lsi09G005750	Watermelon (97103) v1	lsiwmB030
Lsi09G005750	Cucumber (Gy14) v2	cgyblsiB294
Lsi09G005750	Melon (DHL92) v3.6.1	lsimedB013