Lsi09G003480 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi09G003480
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Cytochrome P450, putative
Location	chr09 : 3632340 .. 3634817 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TATTAACACATACCAATTCCATTTGGCTCTTTCTTAACTATTGACCGAAGAAGATGGATTTTAATTTTTTCACCTTCTGTTTCTGCCTTATCTCCACTTTCATAATCCTCCGTCTAATTCCGGCGAGAAGAAACCCCAAACTTCCACCCGGGCCAACACCTCTTCCAATCATCGGAAACCTATTGGATCTTGGACACAACCCCCACAAATCTCTCGCCAAACTTGCCAAGTCTTACGGTCCAATTATGTCGTTAAAGCTTGGTCAAGTCACTGTGGTCGTTGTATCGTCACTGGAGACGATCCAACAAGTCCTTCAAACACATGACCATATCTTATCTTATAGAGCCGTTCCCGATGCTCTAACGGCTTACGACCATGGTCAACTAGGATTCCCATGGATTCCAATTTCCCCCACTTACAAAAACATTTGAAGAATTTGCAACACCTATCTTTTATCTCCCAAGACTCTTGACACCAATCGAAATCTCCGCCGTATGAAGATAGACGACCTTCTCGATAACGTTCGACGAAGCACTGCGAACGGCGAGACGGTGGACATTGGAGGAGTTTTGTTTGCGACGACGGTTAATTTGATATCGTATTCGATTTGGTCTGTGGATTTGGCGGACCCAAATTCTGAAATGGCCAAACAGTTTAAGGCGACTATGAAAGGAATCATGGAAGAAGCTGGAAGACCCAATATTAGTGACTTTTTTCCGGTGCTGAAGAAGATGGATATCCAAGGTATAAGGAGGCGCATTACCGTTCATTTTGCAAAGATGTTTGATTTGATTGATGGGATGATTGATCAACGGCTAAAGATGTAAGAATCGCCTGATTTTACACCCAAAAATGATATGTTACATCATATTCTCAACATGAGGGAGGAGAACAACGAGATCCCTCTTGATAGAAACCAAATCAGACATTCAATATTGGTTAGCCATGTTTTTTCTTATTGATGTTTTGTGTTTTTATTATTCTCTAACATTCTAGTCAAATTCTTACCATAGTTTTTGTCATTCTTAAGTAAAAAAGTTCAATTTTGAGCCAAATTCCAAAAACAAAAATAAGTTTTTACAAACTATTTATTTTAGTTTTCAAAATTTGGTATTTCTGTTTGAAAACTTTGGTAAAAAGTAGATAACAAAGTCAGGAATTTAGAGGTGGAATGAGTGTAATTTTTAGTTTTTGGTTTTAGCAAGTGAGCCTTAAAAAACTACTTCTACCCATAAGTTTGTCATTTTGTAATTCACTTTCTACTATTGCATGCTTTTGAAAATATACTAAGTTTTGAAAACTAAGAGAAGAATTAAGGCCAGTTTGTTAACTATTTAGTTTTTAAAATTCAAATTTATAACATTACTTACACCTATAATTTTTTATGTTTTATTATTCATCTTCTACCACTTAAAAAAAAAAATTAAGTCAAAATTTGAAAGCTAATAAAAGTAGCTTTCAAAAACATTTTGAAAGCTACTTTTATTAGCTTTCAAATTTTGACTTAGTTTATTTTTTTTAGATAACGATAGCATTATTAAATGGGGCCTACATTTTCAAAATTTTATGTTTGTTTTTAAAATTTAACTAGAAATTTAAATATAGGAAATTTTCACATATAGAAAAAATGTCAAACTATTTACAAAAAATAACAAAAAAAAATTGATAAACATTGATATACTTCTATCATTTCTATCACTGATAGATCCTGATAGACTTCTATCAGCGTCTATCACAACTATTTAATTTTTTTTATTTTATATAAATAATTTTCTTTATTTTTTATTTTTAAAAATTTCTCTTTAAATATTCATTTAATAAAGTTCGAAAACCATAATTAGCTATTTGAGAACAAACATAAGCTTGAAAAACATATATAACACTAAAAAACGTAACTATATTATCAAATGAAGTCTTAATTATATAGTCTTGAAAATAAGTACTACACCACATTAAAAGAATTCAAACATAACCAAAGCTTAAAGTAAATTTAATTATATCAATTCTTGAACACAAATTAATCGGAGTTAGAAATGGTTTTAGGTTTTATTTACAGCAAGCACAGATACGACTACATCAATAGTACAATGGGCAATGACGCATATTTTAAAGAACCCAAAAGTTATGTGGAGAGCAAAGGAAGAGATGTTGGGAGTGATAGGAAAAGGGAATTCAATTGAGGAATCACACATCGATAAGCTTCCATATTTACAAGCAATCATCAAAGCAACCTTACGAATGCAATCTGCACTTCTTCTTCCTCGTAAAGCCGAATCACAAGTCACAATTTCAGGCTTCACAATTCCAAAGGGAACTCAAATAATTGTCAATATGTGGGCTTCAGGCAGAGACTCCAATGTATGGGAAAATCCAGATTTGTTCATGCCAGAAAGGTTCTTGGGTTGTGAAACTAAAGGTAGAAATTTTGAGTTCATTCCATTTGGTAGTGGGCGGAGAATTTGCCTGGTCAGCCATTAG

mRNA sequence

TATTAACACATACCAATTCCATTTGGCTCTTTCTTAACTATTGACCGAAGAAGATGGATTTTAATTTTTTCACCTTCTGTTTCTGCCTTATCTCCACTTTCATAATCCTCCGTCTAATTCCGGCGAGAAGAAACCCCAAACTTCCACCCGGGCCAACACCTCTTCCAATCATCGGAAACCTATTGGATCTTGGACACAACCCCCACAAATCTCTCGCCAAACTTGCCAAGTCTTACGGTCCAATTATGTCGTTAAAGCTTGGTCAAGTCACTGTGGTCGTTGTATCGTCACTGGAGACGATCCAACAAGTCCTTCAAACACATGACCATATCTTATCTTATAGAGCCGTTCCCGATGCTCTAACGGCTTACGACCATGGTCAACTAGGATTCCCATGGATTCCAATTTCCCCCACTTACAAAAACATTTGAAGAATTTGCAACACCTATCTTTTATCTCCCAAGACTCTTGACACCAATCGAAATCTCCGCCGTATGAAGATAGACGACCTTCTCGATAACGTTCGACGAAGCACTGCGAACGGCGAGACGGTGGACATTGGAGGAGTTTTGTTTGCGACGACGGTTAATTTGATATCGTATTCGATTTGGTCTGTGGATTTGGCGGACCCAAATTCTGAAATGGCCAAACAGTTTAAGGCGACTATGAAAGGAATCATGGAAGAAGCTGGAAGACCCAATATTAGTGACTTTTTTCCGGTGCTGAAGAAGATGGATATCCAAGGTATAAGGAGGCGCATTACCGTTCATTTTGCAAAGATGTTTGATTTGATTGATGGGATGATTGATCAACGGCTAAAGATTCTTAATTATATAGTCTTGAAAATAACAAGCACAGATACGACTACATCAATAGTACAATGGGCAATGACGCATATTTTAAAGAACCCAAAAGTTATGTGGAGAGCAAAGGAAGAGATGTTGGGAGTGATAGGAAAAGGGAATTCAATTGAGGAATCACACATCGATAAGCTTCCATATTTACAAGCAATCATCAAAGCAACCTTACGAATGCAATCTGCACTTCTTCTTCCTCGTAAAGCCGAATCACAAGTCACAATTTCAGGCTTCACAATTCCAAAGGGAACTCAAATAATTGTCAATATGTGGGCTTCAGGCAGAGACTCCAATGTATGGGAAAATCCAGATTTGTTCATGCCAGAAAGGTTCTTGGGTTGTGAAACTAAAGGTAGAAATTTTGAGTTCATTCCATTTGGTAGTGGGCGGAGAATTTGCCTGGTCAGCCATTAG

Coding sequence (CDS)

ATGAAGATAGACGACCTTCTCGATAACGTTCGACGAAGCACTGCGAACGGCGAGACGGTGGACATTGGAGGAGTTTTGTTTGCGACGACGGTTAATTTGATATCGTATTCGATTTGGTCTGTGGATTTGGCGGACCCAAATTCTGAAATGGCCAAACAGTTTAAGGCGACTATGAAAGGAATCATGGAAGAAGCTGGAAGACCCAATATTAGTGACTTTTTTCCGGTGCTGAAGAAGATGGATATCCAAGGTATAAGGAGGCGCATTACCGTTCATTTTGCAAAGATGTTTGATTTGATTGATGGGATGATTGATCAACGGCTAAAGATTCTTAATTATATAGTCTTGAAAATAACAAGCACAGATACGACTACATCAATAGTACAATGGGCAATGACGCATATTTTAAAGAACCCAAAAGTTATGTGGAGAGCAAAGGAAGAGATGTTGGGAGTGATAGGAAAAGGGAATTCAATTGAGGAATCACACATCGATAAGCTTCCATATTTACAAGCAATCATCAAAGCAACCTTACGAATGCAATCTGCACTTCTTCTTCCTCGTAAAGCCGAATCACAAGTCACAATTTCAGGCTTCACAATTCCAAAGGGAACTCAAATAATTGTCAATATGTGGGCTTCAGGCAGAGACTCCAATGTATGGGAAAATCCAGATTTGTTCATGCCAGAAAGGTTCTTGGGTTGTGAAACTAAAGGTAGAAATTTTGAGTTCATTCCATTTGGTAGTGGGCGGAGAATTTGCCTGGTCAGCCATTAG

Protein sequence

MKIDDLLDNVRRSTANGETVDIGGVLFATTVNLISYSIWSVDLADPNSEMAKQFKATMKGIMEEAGRPNISDFFPVLKKMDIQGIRRRITVHFAKMFDLIDGMIDQRLKILNYIVLKITSTDTTTSIVQWAMTHILKNPKVMWRAKEEMLGVIGKGNSIEESHIDKLPYLQAIIKATLRMQSALLLPRKAESQVTISGFTIPKGTQIIVNMWASGRDSNVWENPDLFMPERFLGCETKGRNFEFIPFGSGRRICLVSH

Homology

[Close]

BLAST of Lsi09G003480 vs. Swiss-Prot
Match: C76B6_CATRO (Geraniol 8-hydroxylase OS=Catharanthus roseus GN=CYP76B6 PE=1 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 2.7e-63
Identity = 122/288 (42.36%), Postives = 187/288 (64.93%), Query Frame = 1


Query: 2   KIDDLLDNVRRSTANGETVDIGGVLFATTVNLISYSIWSVDLADPNSEMAKQFKATMKGI 61
           K+ +L+   R+++ +GE VD+G   F T++NL+S  I+S DL DP S+ AK+FK  +  I
Sbjct: 149 KVQELIAYCRKNSQSGEAVDVGRAAFRTSLNLLSNLIFSKDLTDPYSDSAKEFKDLVWNI 208

Query: 62  MEEAGRPNISDFFPVLKKMDIQGIRRRITVHFAKMFDLIDGMIDQRLK----------IL 121
           M EAG+PN+ DFFP+L+K+D QGIR R+T+HF ++  L  G++++RL+          +L
Sbjct: 209 MVEAGKPNLVDFFPLLEKVDPQGIRHRMTIHFGEVLKLFGGLVNERLEQRRSKGEKNDVL 268

Query: 122 NYIV---------------------LKITSTDTTTSIVQWAMTHILKNPKVMWRAKEEML 181
           + ++                     L +  TDTT+S ++WAM+ +LKNP  M + ++E+ 
Sbjct: 269 DVLLTTSQESPEEIDRTHIERMCLDLFVAGTDTTSSTLEWAMSEMLKNPDKMKKTQDELA 328

Query: 182 GVIGKGNSIEESHIDKLPYLQAIIKATLRMQSAL--LLPRKAESQVTISGFTIPKGTQII 241
            VIG+G +IEES I++LPYL+ ++K TLR+   +  L+PRK E  V + G+ +PKG+Q++
Sbjct: 329 QVIGRGKTIEESDINRLPYLRCVMKETLRIHPPVPFLIPRKVEQSVEVCGYNVPKGSQVL 388

Query: 242 VNMWASGRDSNVWENPDLFMPERFLGCE--TKGRNFEFIPFGSGRRIC 255
           VN WA GRD  VW++   F PERF+  E   +GR+FE IPFG+GRRIC
Sbjct: 389 VNAWAIGRDETVWDDALAFKPERFMESELDIRGRDFELIPFGAGRRIC 436

[Close]

BLAST of Lsi09G003480 vs. Swiss-Prot
Match: C76BA_SWEMU (Geraniol 8-hydroxylase OS=Swertia mussotii GN=CYP76B10 PE=1 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 3.5e-63
Identity = 120/288 (41.67%), Postives = 188/288 (65.28%), Query Frame = 1


Query: 2   KIDDLLDNVRRSTANGETVDIGGVLFATTVNLISYSIWSVDLADPNSEMAKQFKATMKGI 61
           K+ +L+   R+S+  G+ +D+G   F T++NL+S +++S DL DP S+ AK+FK  +  +
Sbjct: 151 KVQELIAYCRKSSQTGDAIDVGRAAFRTSLNLLSNTMFSKDLTDPYSDSAKEFKDLVWNV 210

Query: 62  MEEAGRPNISDFFPVLKKMDIQGIRRRITVHFAKMFDLIDGMIDQRLK----------IL 121
           M EAG+PN+ D+FP+L K+D QGIR+R+T+HF K+ +L  G+ID+RL+          +L
Sbjct: 211 MVEAGKPNLVDYFPLLDKVDPQGIRKRMTIHFGKILELFGGLIDERLQQKKAKGVNDDVL 270

Query: 122 NYIV---------------------LKITSTDTTTSIVQWAMTHILKNPKVMWRAKEEML 181
           + ++                     L +  TDTT+S ++WAM+ +LKNP+ M  A+ E+ 
Sbjct: 271 DVLLTTSEESPEEIDRTHIQRMCLDLFVAGTDTTSSTLEWAMSEMLKNPEKMKAAQAELA 330

Query: 182 GVIGKGNSIEESHIDKLPYLQAIIKATLRMQS--ALLLPRKAESQVTISGFTIPKGTQII 241
            VIGKG ++EE+ + +LPYL+  IK TLR+     LL+PR+ E +V + G+T+PK +Q++
Sbjct: 331 QVIGKGKAVEEADLARLPYLRCAIKETLRIHPPVPLLIPRRTEQEVEVCGYTVPKNSQVL 390

Query: 242 VNMWASGRDSNVWENPDLFMPERFL--GCETKGRNFEFIPFGSGRRIC 255
           VN+WA  RD  +W++P  F PERFL    E +G++FE IPFG+GRRIC
Sbjct: 391 VNVWAISRDDAIWKDPLSFKPERFLESELEMRGKDFELIPFGAGRRIC 438

[Close]

BLAST of Lsi09G003480 vs. Swiss-Prot
Match: C76C3_ARATH (Cytochrome P450 76C3 OS=Arabidopsis thaliana GN=CYP76C3 PE=2 SV=2)

HSP 1 Score: 204.5 bits (519), Expect = 1.4e-51
Identity = 114/295 (38.64%), Postives = 171/295 (57.97%), Query Frame = 1


Query: 2   KIDDLLDNVRRSTANGETVDIGGVLFATTVNLISYSIWSVDLADPNSEMAK-QFKATMKG 61
           K+++L+  V      GE +D+    F T+ N+IS +++SVDLA  +S  +  +F  T+  
Sbjct: 157 KVEELVSLVNEFRERGEAIDLARASFVTSFNIISNALFSVDLATYDSNSSSYEFHNTVVH 216

Query: 62  IMEEAGRPNISDFFPVLKKMDIQGIRRRITVHFAKMFDLIDGMIDQRL------------ 121
           + + AG PN+ D+F  ++ +D+QG R++  +   K+F +    ID RL            
Sbjct: 217 LTDIAGIPNVGDYFQYMRFLDLQGTRKKAVLCIEKLFRVFQEFIDARLAKRFSRTEKEPK 276

Query: 122 ------------------------KILNYIVLKI--TSTDTTTSIVQWAMTHILKNPKVM 181
                                     L +++L +    TDT +S ++WAMT + ++ + M
Sbjct: 277 EASSIDMLDSLLDLTQQNEAELTMNDLKHLLLDVFVAGTDTNSSTMEWAMTELFRSTEKM 336

Query: 182 WRAKEEMLGVIGKGNSIEESHIDKLPYLQAIIKATLRMQSAL-LLPRKAESQVTISGFTI 241
            +A+ E+  VIG+   ++ES I  LPYLQAI+K TLR+  A  L+PRK+ES V I GF +
Sbjct: 337 VKAQSEIRQVIGQNGFVQESDIPSLPYLQAIVKETLRLHPAAPLIPRKSESDVQIMGFLV 396

Query: 242 PKGTQIIVNMWASGRDSNVWENPDLFMPERFLGCET--KGRNFEFIPFGSGRRIC 255
           PK TQ++VN+WA GRD++VWENP  F PERFL  ET  KGR+FE IPFGSGRR+C
Sbjct: 397 PKNTQVVVNVWAIGRDASVWENPMKFEPERFLLRETDVKGRDFELIPFGSGRRMC 451

[Close]

BLAST of Lsi09G003480 vs. Swiss-Prot
Match: C76C1_ARATH (Cytochrome P450 76C1 OS=Arabidopsis thaliana GN=CYP76C1 PE=2 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 1.5e-50
Identity = 112/293 (38.23%), Postives = 172/293 (58.70%), Query Frame = 1


Query: 2   KIDDLLDNVRRSTANGETVDIGGVLFATTVNLISYSIWSVDLADPNSEMA-KQFKATMKG 61
           K+ +L+  +  S+   E+VDI  V F TT+N+IS  ++SVDL   N++ +    + T+  
Sbjct: 158 KVKELVSFISESSDREESVDISRVAFITTLNIISNILFSVDLGSYNAKASINGVQDTVIS 217

Query: 62  IMEEAGRPNISDFFPVLKKMDIQGIRRRITVHFAKMFDLIDGMIDQRL------------ 121
           +M+ AG P+ +++FP L+ +D+QG  +   V   ++  +  G ID ++            
Sbjct: 218 VMDAAGTPDAANYFPFLRFLDLQGNVKTFKVCTERLVRVFRGFIDAKIAEKSSQNNPKDV 277

Query: 122 -------KILNY----------------IVLKITSTDTTTSIVQWAMTHILKNPKVMWRA 181
                   +L+Y                + +    TDT++S ++WAMT +LKNPK M +A
Sbjct: 278 SKNDFVDNLLDYKGDESELSISDIEHLLLDMFTAGTDTSSSTLEWAMTELLKNPKTMAKA 337

Query: 182 KEEMLGVIGKGNSIEESHIDKLPYLQAIIKATLRMQS--ALLLPRKAESQVTISGFTIPK 241
           + E+  VIG+   +EES I KLPYLQA++K T R+ +   LL+PRKAES   I GF + K
Sbjct: 338 QAEIDCVIGQNGIVEESDISKLPYLQAVVKETFRLHTPVPLLIPRKAESDAEILGFMVLK 397

Query: 242 GTQIIVNMWASGRDSNVWENPDLFMPERFLG--CETKGRNFEFIPFGSGRRIC 255
            TQ++VN+WA GRD +VW+NP  F PERFLG   + +GR++E  PFG+GRRIC
Sbjct: 398 DTQVLVNVWAIGRDPSVWDNPSQFEPERFLGKDMDVRGRDYELTPFGAGRRIC 450

[Close]

BLAST of Lsi09G003480 vs. Swiss-Prot
Match: C76A1_SOLME (Cytochrome P450 76A1 (Fragment) OS=Solanum melongena GN=CYP76A1 PE=2 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 4.1e-48
Identity = 110/297 (37.04%), Postives = 166/297 (55.89%), Query Frame = 1


Query: 1   MKIDDLLDNVRRSTANGETVDIGGVLFATTVNLISYSIWSVDLA-DPNSEMAKQFKATMK 60
           +++D+  +    S   GE +++   +F  + N++   I+S DL  DP S+   +F   M 
Sbjct: 117 LRLDE--EKASSSGGGGEGIEVTRYMFLASFNMVGNMIFSKDLVTDPESKQGSEFFNAMI 176

Query: 61  GIMEEAGRPNISDFFPVLKKMDIQGIRRRITVHFAKMFDLIDGMIDQRLK---------- 120
           GIME AG PNISD FP LK  D+QG+R+++     K  ++    I++R++          
Sbjct: 177 GIMEWAGVPNISDIFPCLKMFDVQGLRKKMERDMGKGKEITKKFIEERIEERKKGEKNRS 236

Query: 121 ---ILNYIV---------------LKIT---------STDTTTSIVQWAMTHILKNPKVM 180
              +L+ ++                +IT          T+TT+S V+WA+T +L++P+ M
Sbjct: 237 IKDLLDVLIDFEGSGKDEPDKLSEDEITVIILEMFLAGTETTSSSVEWALTELLRHPQAM 296

Query: 181 WRAKEEMLGVIGKGNSIEESHIDKLPYLQAIIKATLRMQS--ALLLPRKAESQVTISGFT 240
            + K E+L VIG     EE  ID LPY+QA++K  LR+     LL+PRKA       G+ 
Sbjct: 297 AKVKLEILQVIGPNKKFEECDIDSLPYMQAVLKEQLRLHPPLPLLIPRKAIQDTKFMGYD 356

Query: 241 IPKGTQIIVNMWASGRDSNVWENPDLFMPERFL--GCETKGRNFEFIPFGSGRRICL 256
           IPKGTQ++VN WA GRD   W+NP  F PERFL    + KG+N+E IPFG+GRR+C+
Sbjct: 357 IPKGTQVLVNAWAIGRDPEYWDNPFEFKPERFLESKVDVKGQNYELIPFGAGRRMCV 411

[Close]

BLAST of Lsi09G003480 vs. TrEMBL
Match: A0A0A0KKP6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G157320 PE=3 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 1.2e-91
Identity = 177/288 (61.46%), Postives = 209/288 (72.57%), Query Frame = 1


Query: 1   MKIDDLLDNVRRSTANGETVDIGGVLFATTVNLISYSIWSVDLADPNSEMAKQFKATMKG 60
           M+ID  LDN+RR   NGE VDIG  LF+  +NLISYSIWS+DL D +SEM K+FKAT++G
Sbjct: 155 MRIDKHLDNIRRCAVNGEVVDIGTTLFSLALNLISYSIWSMDLVDTDSEMTKEFKATLRG 214

Query: 61  IMEEAGRPNISDFFPVLKKMDIQGIRRRITVHFAKMFDLIDGMIDQRLK----------- 120
            +EE GRPNISDFFPVLKKMD+QG+RRR  +HF KM  LID MID+RLK           
Sbjct: 215 SLEEMGRPNISDFFPVLKKMDVQGVRRRTAIHFGKMLGLIDEMIDKRLKMQETPDFTPKN 274

Query: 121 -----ILNY------------------IVLKITSTDTTTSIVQWAMTHILKNPKVMWRAK 180
                +LN                    VL   S+DTT S+ QWAM ++LK PKVM +AK
Sbjct: 275 DMLHHLLNMKEDNNEIPLDRNQIKHSIFVLFSGSSDTTGSVAQWAMAYLLKYPKVMCKAK 334

Query: 181 EEMLGVIGKGNSIEESHIDKLPYLQAIIKATLRMQSALLLPRKAESQVTISGFTIPKGTQ 240
           EE+L VIGKGN IEESH++KLPYLQAIIK  LRMQS+ L+PRK+ES+VTI GFT+PKGTQ
Sbjct: 335 EELLEVIGKGNPIEESHMEKLPYLQAIIKEVLRMQSSFLIPRKSESEVTILGFTVPKGTQ 394

Query: 241 IIVNMWASGRDSNVWENPDLFMPERFLGCETKGRNFEFIPFGSGRRIC 255
           IIVN+WAS RD N+WENPD+FMPERFL  E+K RNFEFIPF  GRR C
Sbjct: 395 IIVNLWASCRDPNLWENPDVFMPERFL--ESKARNFEFIPFSHGRRTC 440

[Close]

BLAST of Lsi09G003480 vs. TrEMBL
Match: W9S6N5_9ROSA (Cytochrome P450 76C4 OS=Morus notabilis GN=L484_023164 PE=3 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 1.1e-74
Identity = 149/289 (51.56%), Postives = 196/289 (67.82%), Query Frame = 1


Query: 2   KIDDLLDNVRRSTANGETVDIGGVLFATTVNLISYSIWSVDLADPNSEMAKQFKATMKGI 61
           K+ +L + VRRS + GE+VDIG   F TT+NL+S +I+SVDLADP SE+A++FK    GI
Sbjct: 153 KVRELFEGVRRSGSAGESVDIGSEAFTTTLNLLSNTIFSVDLADPKSELAREFKEVAWGI 212

Query: 62  MEEAGRPNISDFFPVLKKMDIQGIRRRITVHFAKMFDLIDGMIDQRLKI----------- 121
           M+EAG PN+ D+FP+L+K+D QGIRRR+TVHF ++  L+DGMI+QRL++           
Sbjct: 213 MKEAGTPNLGDYFPLLRKIDPQGIRRRMTVHFERILSLLDGMINQRLRVRESSGVVHGDV 272

Query: 122 ----LNYI-----------------VLKITSTDTTTSIVQWAMTHILKNPKVMWRAKEEM 181
               LN I                 VL +  TDTT S  QWAM  +L+NP+V+ +A+ E+
Sbjct: 273 LDTLLNIIEEKTEDINRHQVLHFLLVLFVAGTDTTASTFQWAMAELLRNPEVLSKARAEL 332

Query: 182 LGVIGKGNSIEESHIDKLPYLQAIIKATLRMQSA--LLLPRKAESQVTISGFTIPKGTQI 241
             +IGKG  +EES I +LPYLQA++K T R+     LLLPR+AE+ V I GF IPKG QI
Sbjct: 333 NQIIGKGKQVEESDIARLPYLQAVVKETFRLHPVVPLLLPRRAETDVEIGGFKIPKGAQI 392

Query: 242 IVNMWASGRDSNVWENPDLFMPERFLGCE--TKGRNFEFIPFGSGRRIC 255
           +VN WA GRDS  WE+P+ F PERFLG E   +GR+FE IPFG+GRRIC
Sbjct: 393 MVNAWAIGRDSGTWEDPNEFKPERFLGSEIDVRGRSFELIPFGAGRRIC 441

[Close]

BLAST of Lsi09G003480 vs. TrEMBL
Match: A0A061G3V0_THECC (Cytochrome P450, putative OS=Theobroma cacao GN=TCM_016122 PE=3 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 2.2e-72
Identity = 142/290 (48.97%), Postives = 197/290 (67.93%), Query Frame = 1


Query: 2   KIDDLLDNVRRSTANGETVDIGGVLFATTVNLISYSIWSVDLADPNSEMAKQFKATMKGI 61
           KI +L+  V++S   GE ++IG   F TT+NL+S +I+S+DL DPNS  A++FK T+ GI
Sbjct: 154 KIQELIAIVQKSCHAGEVINIGQAAFNTTINLLSNTIFSMDLVDPNSPTAQEFKKTVWGI 213

Query: 62  MEEAGRPNISDFFPVLKKMDIQGIRRRITVHFAKMFDLIDGMIDQRLK------------ 121
           MEEAG+PN++D+FP+L+K+D QG+RRR+T+HF K+ DL   M D+RL+            
Sbjct: 214 MEEAGKPNLADYFPILRKIDPQGVRRRMTIHFEKLLDLFGKMFDERLQSRKAQGSTASND 273

Query: 122 ----ILNYI-----------------VLKITSTDTTTSIVQWAMTHILKNPKVMWRAKEE 181
               +L+ I                 VL +   DTT+S ++WAM  +L+NP+V+ +AK+E
Sbjct: 274 VLDTVLDIIEGNIEELNKTHVTHLLLVLFVAGADTTSSTLEWAMAELLRNPQVLVKAKKE 333

Query: 182 MLGVIGKGNSIEESHIDKLPYLQAIIKATLRMQSA--LLLPRKAESQVTISGFTIPKGTQ 241
           +  VIGKGN +EES I++LPYLQAIIK T RM +   LLLPRKA +   I GFT+PKG Q
Sbjct: 334 LEQVIGKGNPVEESDINRLPYLQAIIKETFRMHATVPLLLPRKAGADAEICGFTVPKGAQ 393

Query: 242 IIVNMWASGRDSNVWENPDLFMPERFLG--CETKGRNFEFIPFGSGRRIC 255
           ++VN+WA GRD ++WE P  FMPERF+G   + KGR+F  IPFG+GRRIC
Sbjct: 394 VLVNVWAIGRDPSIWEKPSAFMPERFMGSDIDVKGRDFGLIPFGAGRRIC 443

[Close]

BLAST of Lsi09G003480 vs. TrEMBL
Match: V4VLS2_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10019826mg PE=3 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 3.2e-71
Identity = 143/290 (49.31%), Postives = 192/290 (66.21%), Query Frame = 1


Query: 2   KIDDLLDNVRRSTANGETVDIGGVLFATTVNLISYSIWSVDLADPNSEMAKQFKATMKGI 61
           KI DLL  V+ +   G  + IG   F TT+NLIS +I+S+DLADP S+ A++FK T  G+
Sbjct: 153 KIQDLLAYVQENCRAGTAIHIGQAAFDTTLNLISNTIFSIDLADPTSKTAREFKQTNWGM 212

Query: 62  MEEAGRPNISDFFPVLKKMDIQGIRRRITVHFAKMFDLIDGMIDQR-------------- 121
           MEEAG+PN+SDFFPVL+ +D+QGIRRR+TV+F KM D+ D +IDQR              
Sbjct: 213 MEEAGKPNLSDFFPVLENLDLQGIRRRMTVYFGKMLDVFDRLIDQRLKQRQERDFTESKD 272

Query: 122 -----LKILN--------------YIVLKITSTDTTTSIVQWAMTHILKNPKVMWRAKEE 181
                LKI                ++VL    TDTT+S ++WAMT +L+NP ++ + + E
Sbjct: 273 MLGALLKICEDKSVEIDINDIKHLFLVLFAAGTDTTSSTLEWAMTELLRNPDILSKVRLE 332

Query: 182 MLGVIGKGNSIEESHIDKLPYLQAIIKATLRMQSA--LLLPRKAESQVTISGFTIPKGTQ 241
           +   IGKGN IEES I +LPYLQA++K T R+  A  LLLPRKA +   ++GF +P G Q
Sbjct: 333 LEQTIGKGNPIEESDITRLPYLQAVLKETFRLHPAVPLLLPRKASADTEVAGFMVPNGAQ 392

Query: 242 IIVNMWASGRDSNVWENPDLFMPERFLG--CETKGRNFEFIPFGSGRRIC 255
           ++VN WA GRD+++W+NP  F+PERFLG   + KGRNFE IPFG+GRRIC
Sbjct: 393 VLVNTWAIGRDASLWDNPYSFVPERFLGLDLDVKGRNFELIPFGAGRRIC 442

[Close]

BLAST of Lsi09G003480 vs. TrEMBL
Match: M5XKJ7_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa023299mg PE=3 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 9.2e-71
Identity = 134/261 (51.34%), Postives = 187/261 (71.65%), Query Frame = 1


Query: 1   MKIDDLLDNVRRSTANGETVDIGGVLFATTVNLISYSIWSVDLADPNSEMAKQFKATMKG 60
           +K+ +L+ +V+ S   G+  ++G   F TT+NL+S +++SVDLADPNSE A++FK  +  
Sbjct: 151 LKVQELIADVKESVVKGKAAEVGRAAFKTTLNLMSRTVFSVDLADPNSERAREFKELVWS 210

Query: 61  IMEEAGRPNISDFFPVLKKMDIQGIRRRITVHFAKMFDLIDGMIDQRL---KILNYIVLK 120
           IMEEAG+PN++D+FPVLKK+D  GIRRR+  HF KM DL D MI QRL   K  +Y +  
Sbjct: 211 IMEEAGKPNLADYFPVLKKIDPMGIRRRLGKHFQKMIDLFDRMIVQRLESRKSRDYDLFG 270

Query: 121 ITSTDTTTSIVQWAMTHILKNPKVMWRAKEEMLGVIGKGNSIEESHIDKLPYLQAIIKAT 180
              TDTT++ ++WAM  +L+NP+ + +A+EE+  V+GKG  +EES I +LPYLQAIIK T
Sbjct: 271 -AGTDTTSATLEWAMAELLRNPEKLSKAQEELKQVVGKGKPVEESDITRLPYLQAIIKET 330

Query: 181 LRMQSA--LLLPRKAESQVTISGFTIPKGTQIIVNMWASGRDSNVWENPDLFMPERFLG- 240
            R+     LLLPRKA++ + I G+ +PKG Q++VN WA GRD ++W+NP  F+PERF G 
Sbjct: 331 FRLHPVVPLLLPRKAQADIEICGYIVPKGVQVLVNAWAIGRDPSIWDNPTSFIPERFSGL 390

Query: 241 -CETKGRNFEFIPFGSGRRIC 255
             +  G+NFE IPFG GRRIC
Sbjct: 391 DIDVTGQNFELIPFGGGRRIC 410

[Close]

BLAST of Lsi09G003480 vs. TAIR10
Match: AT2G45580.1 (AT2G45580.1 cytochrome P450, family 76, subfamily C, polypeptide 3)

HSP 1 Score: 204.5 bits (519), Expect = 7.7e-53
Identity = 114/295 (38.64%), Postives = 171/295 (57.97%), Query Frame = 1


Query: 2   KIDDLLDNVRRSTANGETVDIGGVLFATTVNLISYSIWSVDLADPNSEMAK-QFKATMKG 61
           K+++L+  V      GE +D+    F T+ N+IS +++SVDLA  +S  +  +F  T+  
Sbjct: 157 KVEELVSLVNEFRERGEAIDLARASFVTSFNIISNALFSVDLATYDSNSSSYEFHNTVVH 216

Query: 62  IMEEAGRPNISDFFPVLKKMDIQGIRRRITVHFAKMFDLIDGMIDQRL------------ 121
           + + AG PN+ D+F  ++ +D+QG R++  +   K+F +    ID RL            
Sbjct: 217 LTDIAGIPNVGDYFQYMRFLDLQGTRKKAVLCIEKLFRVFQEFIDARLAKRFSRTEKEPK 276

Query: 122 ------------------------KILNYIVLKI--TSTDTTTSIVQWAMTHILKNPKVM 181
                                     L +++L +    TDT +S ++WAMT + ++ + M
Sbjct: 277 EASSIDMLDSLLDLTQQNEAELTMNDLKHLLLDVFVAGTDTNSSTMEWAMTELFRSTEKM 336

Query: 182 WRAKEEMLGVIGKGNSIEESHIDKLPYLQAIIKATLRMQSAL-LLPRKAESQVTISGFTI 241
            +A+ E+  VIG+   ++ES I  LPYLQAI+K TLR+  A  L+PRK+ES V I GF +
Sbjct: 337 VKAQSEIRQVIGQNGFVQESDIPSLPYLQAIVKETLRLHPAAPLIPRKSESDVQIMGFLV 396

Query: 242 PKGTQIIVNMWASGRDSNVWENPDLFMPERFLGCET--KGRNFEFIPFGSGRRIC 255
           PK TQ++VN+WA GRD++VWENP  F PERFL  ET  KGR+FE IPFGSGRR+C
Sbjct: 397 PKNTQVVVNVWAIGRDASVWENPMKFEPERFLLRETDVKGRDFELIPFGSGRRMC 451

[Close]

BLAST of Lsi09G003480 vs. TAIR10
Match: AT2G45560.1 (AT2G45560.1 cytochrome P450, family 76, subfamily C, polypeptide 1)

HSP 1 Score: 201.1 bits (510), Expect = 8.6e-52
Identity = 112/293 (38.23%), Postives = 172/293 (58.70%), Query Frame = 1


Query: 2   KIDDLLDNVRRSTANGETVDIGGVLFATTVNLISYSIWSVDLADPNSEMA-KQFKATMKG 61
           K+ +L+  +  S+   E+VDI  V F TT+N+IS  ++SVDL   N++ +    + T+  
Sbjct: 158 KVKELVSFISESSDREESVDISRVAFITTLNIISNILFSVDLGSYNAKASINGVQDTVIS 217

Query: 62  IMEEAGRPNISDFFPVLKKMDIQGIRRRITVHFAKMFDLIDGMIDQRL------------ 121
           +M+ AG P+ +++FP L+ +D+QG  +   V   ++  +  G ID ++            
Sbjct: 218 VMDAAGTPDAANYFPFLRFLDLQGNVKTFKVCTERLVRVFRGFIDAKIAEKSSQNNPKDV 277

Query: 122 -------KILNY----------------IVLKITSTDTTTSIVQWAMTHILKNPKVMWRA 181
                   +L+Y                + +    TDT++S ++WAMT +LKNPK M +A
Sbjct: 278 SKNDFVDNLLDYKGDESELSISDIEHLLLDMFTAGTDTSSSTLEWAMTELLKNPKTMAKA 337

Query: 182 KEEMLGVIGKGNSIEESHIDKLPYLQAIIKATLRMQS--ALLLPRKAESQVTISGFTIPK 241
           + E+  VIG+   +EES I KLPYLQA++K T R+ +   LL+PRKAES   I GF + K
Sbjct: 338 QAEIDCVIGQNGIVEESDISKLPYLQAVVKETFRLHTPVPLLIPRKAESDAEILGFMVLK 397

Query: 242 GTQIIVNMWASGRDSNVWENPDLFMPERFLG--CETKGRNFEFIPFGSGRRIC 255
            TQ++VN+WA GRD +VW+NP  F PERFLG   + +GR++E  PFG+GRRIC
Sbjct: 398 DTQVLVNVWAIGRDPSVWDNPSQFEPERFLGKDMDVRGRDYELTPFGAGRRIC 450

[Close]

BLAST of Lsi09G003480 vs. TAIR10
Match: AT3G61040.1 (AT3G61040.1 cytochrome P450, family 76, subfamily C, polypeptide 7)

HSP 1 Score: 196.1 bits (497), Expect = 2.8e-50
Identity = 109/288 (37.85%), Postives = 170/288 (59.03%), Query Frame = 1


Query: 2   KIDDLLDNVRRSTANGETVDIGGVLFATTVNLISYSIWSVDLAD-PNSEMAKQFKATMKG 61
           K+ +L++ V +     E V+I    F T++N+IS +++S +LA+  +S+    F+  +  
Sbjct: 152 KVQELMNFVNKCCERREAVNISRASFITSLNIISNALFSTNLANFDDSKTFHDFQNVVIR 211

Query: 62  IMEEAGRPNISDFFPVLKKMDIQGIRRRITVHFAKMFDLIDGMIDQRLKILN-------- 121
           +ME +G+PN++DFFP L  +D+QG R+   +   K+F +  G ID +    +        
Sbjct: 212 MMEISGKPNLADFFPFLGFLDLQGARKEARLLMHKLFRVFQGFIDTKRSSTSRNNNDMLD 271

Query: 122 ---------------------YIVLKITSTDTTTSIVQWAMTHILKNPKVMWRAKEEMLG 181
                                 + L +   DT++S V+WAM  +L+NPK++ + +EE+  
Sbjct: 272 SLLDIAHKKESELDDNNIKHLLLDLFLAGVDTSSSAVEWAMAELLRNPKMIVKVQEEIRQ 331

Query: 182 VIGKGNSIEESHIDKLPYLQAIIKATLRMQ--SALLLPRKAES-QVTISGFTIPKGTQII 241
           VIG   ++++  I KLPYLQA++K +LR+   +  L+PRK+ES  V I  F IPK TQ++
Sbjct: 332 VIGLKGTVQDLDIVKLPYLQAVVKESLRLHPPAPFLVPRKSESDDVQIFEFLIPKNTQVL 391

Query: 242 VNMWASGRDSNVWENPDLFMPERFL--GCETKGRNFEFIPFGSGRRIC 255
           VN+WA GRD NVW+NP  F PERFL  G + KG +FE IPFG+GRRIC
Sbjct: 392 VNVWAIGRDPNVWKNPTQFEPERFLGRGIDVKGNHFELIPFGAGRRIC 439

[Close]

BLAST of Lsi09G003480 vs. TAIR10
Match: AT1G33720.1 (AT1G33720.1 cytochrome P450, family 76, subfamily C, polypeptide 6)

HSP 1 Score: 191.0 bits (484), Expect = 8.9e-49
Identity = 107/293 (36.52%), Postives = 166/293 (56.66%), Query Frame = 1


Query: 2   KIDDLLDNVRRSTANGETVDIGGVLFATTVNLISYSIWSVDLADPNSEMAKQFKATMKGI 61
           K+ +L++ +  S    E VDI  V F T +N+IS  ++SV+L   +S+ +  F+  + G 
Sbjct: 158 KVQELVNFLSESCEREEAVDISHVSFVTALNIISNILFSVNLGSYDSKNSSAFQEMVIGY 217

Query: 62  MEEAGRPNISDFFPVLKKMDIQGIRRRITVHFAKMF------------------------ 121
            E  G P++++FFP ++ +D+QG  +++     ++                         
Sbjct: 218 QESIGNPDLANFFPFMRFLDLQGNSKKMRESSGRLLQVFREFYDARIVEKSSRSVEKDVS 277

Query: 122 --DLIDGMIDQR--------LKILNYIVLK--ITSTDTTTSIVQWAMTHILKNPKVMWRA 181
             D +D +ID +        +  + +++L   +  TDT +S V+WAM  +L NPK M + 
Sbjct: 278 SKDFLDVLIDLQQGDETEINIDEIEHLLLDMFVAGTDTNSSTVEWAMAELLGNPKTMTKV 337

Query: 182 KEEMLGVIGKGNSIEESHIDKLPYLQAIIKATLRMQSA--LLLPRKAESQVTISGFTIPK 241
           ++E+  VIG+    +ES I KLPYL+A++K T R+  A   LL RKAE+ V I GFT+ K
Sbjct: 338 QDEINHVIGQNGDFQESDISKLPYLKAVVKETFRLHPAAPFLLQRKAETNVEILGFTVLK 397

Query: 242 GTQIIVNMWASGRDSNVWENPDLFMPERFLGCE--TKGRNFEFIPFGSGRRIC 255
            +Q++VN+WA GRD  VWENP  F PERFLG E   KG ++E  PFG+GRRIC
Sbjct: 398 DSQVLVNVWAIGRDPLVWENPTHFEPERFLGKEIDVKGTDYELTPFGAGRRIC 450

[Close]

BLAST of Lsi09G003480 vs. TAIR10
Match: AT1G33730.1 (AT1G33730.1 cytochrome P450, family 76, subfamily C, polypeptide 5)

HSP 1 Score: 186.0 bits (471), Expect = 2.8e-47
Identity = 108/293 (36.86%), Postives = 163/293 (55.63%), Query Frame = 1


Query: 2   KIDDLLDNVRRSTANGETVDIGGVLFATTVNLISYSIWSVDLADPNSEMAKQFKATMKGI 61
           K+ +L++ +  S+  GE VDI    F T +N+IS  ++SV+L   +S+ +  F+  + G 
Sbjct: 22  KVQELVNFLSESSERGEAVDISRASFVTALNIISNILFSVNLGSYDSKNSSAFQEMVIGY 81

Query: 62  MEEAGRPNISDFFPVLKKMDIQGIRRRITVHFAKMFDLIDGMIDQR-LKILNYIVLK-IT 121
           ME  G P++S+FFP ++ +D+QG  +++  +  K+  +     D R L+  + I  K ++
Sbjct: 82  MESIGNPDVSNFFPFMRLLDLQGNSKKMKEYSGKLLQVFREFYDARILENSSRIDEKDVS 141

Query: 122 STDTTTSIVQW-----------AMTHILK-----------------------NPKVMWRA 181
           S D   +++              + H+L                        NPK M + 
Sbjct: 142 SRDFLDALIDLQQGDESEINIDEIEHLLLDMFLAGTDTNSSTVEWAMTELLGNPKTMTKV 201

Query: 182 KEEMLGVIGKGNSIEESHIDKLPYLQAIIKATLRMQSA--LLLPRKAESQVTISGFTIPK 241
           ++E+  VI +   ++ESHI KLPYLQA+IK T R+  A   LLPRKAE  V I GF +PK
Sbjct: 202 QDEINRVIRQNGDVQESHISKLPYLQAVIKETFRLHPAAPFLLPRKAERDVDILGFHVPK 261

Query: 242 GTQIIVNMWASGRDSNVWENPDLFMPERFLG--CETKGRNFEFIPFGSGRRIC 255
            + ++VN+WA GRD NVWENP  F PERFLG   + KG N+E  PFG+GRRIC
Sbjct: 262 DSHVLVNVWAIGRDPNVWENPTQFEPERFLGKDIDVKGTNYELTPFGAGRRIC 314

[Close]

BLAST of Lsi09G003480 vs. NCBI nr
Match: gi|659073646|ref|XP_008437175.1| (PREDICTED: geraniol 8-hydroxylase-like [Cucumis melo])

HSP 1 Score: 350.1 bits (897), Expect = 3.2e-93
Identity = 179/288 (62.15%), Postives = 211/288 (73.26%), Query Frame = 1


Query: 1   MKIDDLLDNVRRSTANGETVDIGGVLFATTVNLISYSIWSVDLADPNSEMAKQFKATMKG 60
           M+ID LLDN+RR   NGE VD+G  LF+  +NLISYSIWS+DL D +SEM K+FK T++G
Sbjct: 149 MRIDKLLDNIRRCAVNGEVVDVGRTLFSMALNLISYSIWSMDLVDTDSEMIKEFKTTLRG 208

Query: 61  IMEEAGRPNISDFFPVLKKMDIQGIRRRITVHFAKMFDLIDGMIDQRLK----------- 120
            +EE GRPNISDFFPVLKKMDIQG+RRR  VHF K+  LID MID+RLK           
Sbjct: 209 SLEEMGRPNISDFFPVLKKMDIQGVRRRTAVHFGKILGLIDEMIDKRLKMQESPDFTPKN 268

Query: 121 -----ILNY------------------IVLKITSTDTTTSIVQWAMTHILKNPKVMWRAK 180
                +LN                    VL     D   SI QWAM ++LKNPKVM +AK
Sbjct: 269 DMLHHLLNMKEDNNQIPLDRNQIKHSIFVLFTAGPDAAGSIAQWAMAYLLKNPKVMCKAK 328

Query: 181 EEMLGVIGKGNSIEESHIDKLPYLQAIIKATLRMQSALLLPRKAESQVTISGFTIPKGTQ 240
           EE++ VIGKGNSIEESH++KLPYLQAIIK  LRMQS+LL+PR+AES+VTISGFTIPKG Q
Sbjct: 329 EELMEVIGKGNSIEESHMEKLPYLQAIIKEALRMQSSLLIPRRAESEVTISGFTIPKGAQ 388

Query: 241 IIVNMWASGRDSNVWENPDLFMPERFLGCETKGRNFEFIPFGSGRRIC 255
           IIVN+WAS RD N+WENPD+F+PERFL  E+KGRNFEFIPFG+GRR C
Sbjct: 389 IIVNLWASCRDPNLWENPDMFIPERFL--ESKGRNFEFIPFGNGRRTC 434

[Close]

BLAST of Lsi09G003480 vs. NCBI nr
Match: gi|449459736|ref|XP_004147602.1| (PREDICTED: geraniol 8-hydroxylase [Cucumis sativus])

HSP 1 Score: 344.4 bits (882), Expect = 1.8e-91
Identity = 177/288 (61.46%), Postives = 209/288 (72.57%), Query Frame = 1


Query: 1   MKIDDLLDNVRRSTANGETVDIGGVLFATTVNLISYSIWSVDLADPNSEMAKQFKATMKG 60
           M+ID  LDN+RR   NGE VDIG  LF+  +NLISYSIWS+DL D +SEM K+FKAT++G
Sbjct: 155 MRIDKHLDNIRRCAVNGEVVDIGTTLFSLALNLISYSIWSMDLVDTDSEMTKEFKATLRG 214

Query: 61  IMEEAGRPNISDFFPVLKKMDIQGIRRRITVHFAKMFDLIDGMIDQRLK----------- 120
            +EE GRPNISDFFPVLKKMD+QG+RRR  +HF KM  LID MID+RLK           
Sbjct: 215 SLEEMGRPNISDFFPVLKKMDVQGVRRRTAIHFGKMLGLIDEMIDKRLKMQETPDFTPKN 274

Query: 121 -----ILNY------------------IVLKITSTDTTTSIVQWAMTHILKNPKVMWRAK 180
                +LN                    VL   S+DTT S+ QWAM ++LK PKVM +AK
Sbjct: 275 DMLHHLLNMKEDNNEIPLDRNQIKHSIFVLFSGSSDTTGSVAQWAMAYLLKYPKVMCKAK 334

Query: 181 EEMLGVIGKGNSIEESHIDKLPYLQAIIKATLRMQSALLLPRKAESQVTISGFTIPKGTQ 240
           EE+L VIGKGN IEESH++KLPYLQAIIK  LRMQS+ L+PRK+ES+VTI GFT+PKGTQ
Sbjct: 335 EELLEVIGKGNPIEESHMEKLPYLQAIIKEVLRMQSSFLIPRKSESEVTILGFTVPKGTQ 394

Query: 241 IIVNMWASGRDSNVWENPDLFMPERFLGCETKGRNFEFIPFGSGRRIC 255
           IIVN+WAS RD N+WENPD+FMPERFL  E+K RNFEFIPF  GRR C
Sbjct: 395 IIVNLWASCRDPNLWENPDVFMPERFL--ESKARNFEFIPFSHGRRTC 440

[Close]

BLAST of Lsi09G003480 vs. NCBI nr
Match: gi|778699842|ref|XP_011654780.1| (PREDICTED: geraniol 8-hydroxylase-like [Cucumis sativus])

HSP 1 Score: 328.9 bits (842), Expect = 7.7e-87
Identity = 167/290 (57.59%), Postives = 211/290 (72.76%), Query Frame = 1


Query: 2   KIDDLLDNVRRSTANGETVDIGGVLFATTVNLISYSIWSVDLADPNSEMAKQFKATMKGI 61
           KI +LL++VR S   GE VD+G   F T +N++S SIWS+DLAD NSEM KQF++  +G+
Sbjct: 150 KIKELLNSVRESAVKGEVVDVGAAAFRTALNIMSCSIWSMDLADTNSEMVKQFQSRFRGV 209

Query: 62  MEEAGRPNISDFFPVLKKMDIQGIRRRITVHFAKMFDLIDGMIDQRLKI----------- 121
           ME  GR N+SDFFPV+KK DIQG+RRR +VH  K+FDLID MID RLK+           
Sbjct: 210 MEAFGRTNVSDFFPVVKKFDIQGVRRRNSVHLRKIFDLIDEMIDGRLKMQESLGFTPKID 269

Query: 122 ----------------------LNYIVLK--ITSTDTTTSIVQWAMTHILKNPKVMWRAK 181
                                 + +++L   +  TDTT +I+QWAM ++L+NPKVM +AK
Sbjct: 270 ALYHLLNLDEEKNGEILMDRNQIGHLILDLFVAGTDTTATIIQWAMAYLLQNPKVMSKAK 329

Query: 182 EEMLGVIGKGNSI-EESHIDKLPYLQAIIKATLRMQSALLLPRKAESQVTISGFTIPKGT 241
           +E+  +IGKGN+I EES I+KLPYLQAIIK TLR+QS+LLLPRKA+SQVTISG+T+PKGT
Sbjct: 330 DELNQMIGKGNAIIEESQIEKLPYLQAIIKETLRLQSSLLLPRKAQSQVTISGYTVPKGT 389

Query: 242 QIIVNMWASGRDSNVWENPDLFMPERFLG-CETKGRNFEFIPFGSGRRIC 255
           QIIVN+WA GRDSN+WE P+ F+PERF G   TKGRNFE+IPFGSGRRIC
Sbjct: 390 QIIVNLWALGRDSNIWEQPNCFIPERFFGNFNTKGRNFEYIPFGSGRRIC 439

[Close]

BLAST of Lsi09G003480 vs. NCBI nr
Match: gi|659073640|ref|XP_008437172.1| (PREDICTED: geraniol 8-hydroxylase-like [Cucumis melo])

HSP 1 Score: 320.1 bits (819), Expect = 3.6e-84
Identity = 165/290 (56.90%), Postives = 207/290 (71.38%), Query Frame = 1


Query: 2   KIDDLLDNVRRSTANGETVDIGGVLFATTVNLISYSIWSVDLADPNSEMAKQFKATMKGI 61
           KI+DLL +VR+    GE VD+G   F   +N++S SIWS+DLAD NSEM KQFK+   G+
Sbjct: 150 KIEDLLSSVRQRAVKGEVVDVGAAAFTAVLNMMSCSIWSMDLADTNSEMVKQFKSRFGGV 209

Query: 62  MEEAGRPNISDFFPVLKKMDIQGIRRRITVHFAKMFDLIDGMIDQRLKI----------- 121
           ME  G+ N+SDFFPV+KK DIQG+RRR +V   +MF LID MID RLK+           
Sbjct: 210 MEAFGKTNVSDFFPVVKKFDIQGVRRRNSVRLREMFVLIDEMIDGRLKMQESLDFTPKFD 269

Query: 122 ----------------------LNYIVLK--ITSTDTTTSIVQWAMTHILKNPKVMWRAK 181
                                 + +++L   +  TDT+ +I+QWAM ++L+NPKVM +AK
Sbjct: 270 ALHHLLNLDEEKNGEFLLDRNQIRHLILDLFVAGTDTSATIIQWAMAYLLQNPKVMSKAK 329

Query: 182 EEMLGVIGKGNSI-EESHIDKLPYLQAIIKATLRMQSALLLPRKAESQVTISGFTIPKGT 241
           EE+  +IGKGN+I EES I+KLPYLQAIIK TLR+QS+LLLPRK++SQVTISG+TIPKGT
Sbjct: 330 EELNQIIGKGNAIIEESQIEKLPYLQAIIKETLRLQSSLLLPRKSQSQVTISGYTIPKGT 389

Query: 242 QIIVNMWASGRDSNVWENPDLFMPERFLG-CETKGRNFEFIPFGSGRRIC 255
           QIIVN+WA GRDSN+WE P+ FMPERFLG   TKGR+F FIPFGSGRRIC
Sbjct: 390 QIIVNLWALGRDSNIWEQPNCFMPERFLGNLNTKGRDFGFIPFGSGRRIC 439

[Close]

BLAST of Lsi09G003480 vs. NCBI nr
Match: gi|659073642|ref|XP_008437173.1| (PREDICTED: geraniol 8-hydroxylase-like isoform X1 [Cucumis melo])

HSP 1 Score: 306.6 bits (784), Expect = 4.1e-80
Identity = 161/290 (55.52%), Postives = 202/290 (69.66%), Query Frame = 1


Query: 1   MKIDDLLDNVRRSTANGETVDIGGVLFATTVNLISYSIWSVDLADPNSEMAKQFKATMKG 60
           ++ID LLDN+RR   NGE VDIG  LF+  +NLISY+IWSVDL D +SEM K+FK T++G
Sbjct: 150 LRIDKLLDNIRRCAVNGEVVDIGRTLFSMALNLISYTIWSVDLVDEDSEMTKEFKTTLRG 209

Query: 61  IMEEAGRPNISDFFPVLKKMDIQGIRRRITVHFAKMF----------------------- 120
            +EE GRPNISDFFP+LKKMDIQG+RRR TVHF K+                        
Sbjct: 210 SLEEIGRPNISDFFPMLKKMDIQGVRRRTTVHFGKILSLIDEMIDKRLKMQESPDFTPKN 269

Query: 121 DLIDGMIDQ----------RLKILNYIVLKIT-STDTTTSIVQWAMTHILKNPKVMWRAK 180
           DL+  +++           R +I + I +  T S+DTT S+ QW M ++LKNPK+M +AK
Sbjct: 270 DLLHYLLNMKEDNSGIPLDRNQIKHSIFVLFTGSSDTTGSVAQWIMAYLLKNPKLMCKAK 329

Query: 181 EEMLGVIGKGNSIEESHIDKLPYLQAIIKATLRMQSALLLPRKAESQVTISGFTIPKGTQ 240
           EE++ VIGKGN IEESH++KLPYLQAIIK  LRMQS+ L+PRKAES+V ISGFTIPKGTQ
Sbjct: 330 EELMEVIGKGNRIEESHMEKLPYLQAIIKEALRMQSSFLIPRKAESEVIISGFTIPKGTQ 389

Query: 241 IIVNMWASGRDSNVWENPDLFMPERFLGCETKGRNFEFIPFGSGRRICLV 257
           I+VN+WAS RD N+WENPD F+PERFL  E+KG+         GR + LV
Sbjct: 390 ILVNLWASCRDPNLWENPDEFIPERFL--ESKGKILSLFHLVMGRGLVLV 437

The following BLAST results are available for this feature:

BLAST of Lsi09G003480 vs. Swiss-Prot


Match Name	E-value	Identity	Description
C76B6_CATRO	2.7e-63	42.36	Geraniol 8-hydroxylase OS=Catharanthus roseus GN=CYP76B6 PE=1 SV=1	[more]
C76BA_SWEMU	3.5e-63	41.67	Geraniol 8-hydroxylase OS=Swertia mussotii GN=CYP76B10 PE=1 SV=1	[more]
C76C3_ARATH	1.4e-51	38.64	Cytochrome P450 76C3 OS=Arabidopsis thaliana GN=CYP76C3 PE=2 SV=2	[more]
C76C1_ARATH	1.5e-50	38.23	Cytochrome P450 76C1 OS=Arabidopsis thaliana GN=CYP76C1 PE=2 SV=1	[more]
C76A1_SOLME	4.1e-48	37.04	Cytochrome P450 76A1 (Fragment) OS=Solanum melongena GN=CYP76A1 PE=2 SV=1	[more]

BLAST of Lsi09G003480 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KKP6_CUCSA	1.2e-91	61.46	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G157320 PE=3 SV=1	[more]
W9S6N5_9ROSA	1.1e-74	51.56	Cytochrome P450 76C4 OS=Morus notabilis GN=L484_023164 PE=3 SV=1	[more]
A0A061G3V0_THECC	2.2e-72	48.97	Cytochrome P450, putative OS=Theobroma cacao GN=TCM_016122 PE=3 SV=1	[more]
V4VLS2_9ROSI	3.2e-71	49.31	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10019826mg PE=3 SV=1	[more]
M5XKJ7_PRUPE	9.2e-71	51.34	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa023299mg PE=3 SV=1	[more]

BLAST of Lsi09G003480 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G45580.1	7.7e-53	38.64	cytochrome P450, family 76, subfamily C, polypeptide 3	[more]
AT2G45560.1	8.6e-52	38.23	cytochrome P450, family 76, subfamily C, polypeptide 1	[more]
AT3G61040.1	2.8e-50	37.85	cytochrome P450, family 76, subfamily C, polypeptide 7	[more]
AT1G33720.1	8.9e-49	36.52	cytochrome P450, family 76, subfamily C, polypeptide 6	[more]
AT1G33730.1	2.8e-47	36.86	cytochrome P450, family 76, subfamily C, polypeptide 5	[more]

BLAST of Lsi09G003480 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659073646\|ref\|XP_008437175.1\|	3.2e-93	62.15	PREDICTED: geraniol 8-hydroxylase-like [Cucumis melo]	[more]
gi\|449459736\|ref\|XP_004147602.1\|	1.8e-91	61.46	PREDICTED: geraniol 8-hydroxylase [Cucumis sativus]	[more]
gi\|778699842\|ref\|XP_011654780.1\|	7.7e-87	57.59	PREDICTED: geraniol 8-hydroxylase-like [Cucumis sativus]	[more]
gi\|659073640\|ref\|XP_008437172.1\|	3.6e-84	56.90	PREDICTED: geraniol 8-hydroxylase-like [Cucumis melo]	[more]
gi\|659073642\|ref\|XP_008437173.1\|	4.1e-80	55.52	PREDICTED: geraniol 8-hydroxylase-like isoform X1 [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0020037	heme binding
GO:0005506	iron ion binding
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Vocabulary: **INTERPRO**
Term	Definition
IPR002401	Cyt_P450_E_grp-I
IPR001128	Cyt_P450

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi09G003480.1	Lsi09G003480.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 245..254 score: 1.8E-6coord: 119..136 score: 1.8E-6coord: 172..183 score: 1.
IPR001128	Cytochrome P450	GENE3D	G3DSA:1.10.630.10		coord: 2..255 score: 3.4
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 118..255 score: 1.1E-45coord: 3..111 score: 9.
IPR001128	Cytochrome P450	unknown	SSF48264	Cytochrome P450	coord: 3..255 score: 9.95
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 128..154 score: 1.1E-17coord: 244..254 score: 1.1E-17coord: 210..234 score: 1.1
None	No IPR available	PANTHER	PTHR24298	FAMILY NOT NAMED	coord: 1..255 score: 1.1E
None	No IPR available	PANTHER	PTHR24298:SF121	CYTOCHROME P450 76C3	coord: 1..255 score: 1.1E

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi09G003480	Cucumber (Chinese Long) v3	cuclsiB171
Lsi09G003480	Cucumber (Chinese Long) v3	cuclsiB345
Lsi09G003480	Watermelon (97103) v2	lsiwmbB007
Lsi09G003480	Wax gourd	lsiwgoB010
Lsi09G003480	Bottle gourd (USVL1VR-Ls)	lsilsiB008
Lsi09G003480	Cucumber (Gy14) v1	cgylsiB411
Lsi09G003480	Cucumber (Gy14) v1	cgylsiB487
Lsi09G003480	Cucurbita maxima (Rimu)	cmalsiB267
Lsi09G003480	Cucurbita maxima (Rimu)	cmalsiB365
Lsi09G003480	Cucurbita maxima (Rimu)	cmalsiB530
Lsi09G003480	Cucurbita moschata (Rifu)	cmolsiB255
Lsi09G003480	Cucurbita moschata (Rifu)	cmolsiB353
Lsi09G003480	Cucurbita moschata (Rifu)	cmolsiB516
Lsi09G003480	Cucurbita pepo (Zucchini)	cpelsiB131
Lsi09G003480	Cucurbita pepo (Zucchini)	cpelsiB581
Lsi09G003480	Wild cucumber (PI 183967)	cpilsiB155
Lsi09G003480	Wild cucumber (PI 183967)	cpilsiB322
Lsi09G003480	Cucumber (Chinese Long) v2	culsiB156
Lsi09G003480	Cucumber (Chinese Long) v2	culsiB322
Lsi09G003480	Melon (DHL92) v3.5.1	lsimeB012
Lsi09G003480	Melon (DHL92) v3.5.1	lsimeB036
Lsi09G003480	Watermelon (Charleston Gray)	lsiwcgB008
Lsi09G003480	Watermelon (Charleston Gray)	lsiwcgB023
Lsi09G003480	Watermelon (97103) v1	lsiwmB024
Lsi09G003480	Watermelon (97103) v1	lsiwmB027
Lsi09G003480	Cucumber (Gy14) v2	cgyblsiB141
Lsi09G003480	Cucumber (Gy14) v2	cgyblsiB294
Lsi09G003480	Melon (DHL92) v3.6.1	lsimedB012
Lsi09G003480	Melon (DHL92) v3.6.1	lsimedB036
Lsi09G003480	Silver-seed gourd	carlsiB851