Lsi09G003420 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi09G003420
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Subtilisin-like serine protease
Location	chr09 : 3543450 .. 3544001 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACTACTGCAAACATAAAAACTCCTCAAGGAAAACCAATTCTGGATCAAGATTTGAAACCGGCTAACTTTTTTGCAATTGGTGCTGGACATGTAAACCCATCAAAGGCAGCTGACCCAGGATTGGTTTATGACATTCAATCTGATGATTACATTCCTTATCTTTGTGGTTTGTACAAAGATGAAGAAGTTTCAATTATTGTTCATAAAACAGTAATCTGCGGATTAGTACCAAGGATTCGAGAAGGGGATTTGAATTATCCTTCGTTTAGTGTCGAGTTAGGAGGCTTACAAACGTTCAAAAGAACAGTGACAAATGTGGGTGAAGCAAACTCGGTTTATTCTGTCATGGTTGAAGCACCGCTGGGAGTTTCTATGACGGTCTTACCAAGAAAATTGGTGTTCTCGAGGGTGAACCAAAAGTTGACCTTTTCGGTGACTTTTAGTCGAATTAGCTCTGTTAAAATAATAGGTGAATTTGGTCAAGGATATCTCAAATGGGTTTCTCAAAAACATGTTGTGAGGAGTCCCATTTCTGTGAAGTTTAATTAA

mRNA sequence

ATGACTACTGCAAACATAAAAACTCCTCAAGGAAAACCAATTCTGGATCAAGATTTGAAACCGGCTAACTTTTTTGCAATTGGTGCTGGACATGTAAACCCATCAAAGGCAGCTGACCCAGGATTGGTTTATGACATTCAATCTGATGATTACATTCCTTATCTTTGTGGTTTGTACAAAGATGAAGAAGTTTCAATTATTGTTCATAAAACAGTAATCTGCGGATTAGTACCAAGGATTCGAGAAGGGGATTTGAATTATCCTTCGTTTAGTGTCGAGTTAGGAGGCTTACAAACGTTCAAAAGAACAGTGACAAATGTGGGTGAAGCAAACTCGGTTTATTCTGTCATGGTTGAAGCACCGCTGGGAGTTTCTATGACGGTCTTACCAAGAAAATTGGTGTTCTCGAGGGTGAACCAAAAGTTGACCTTTTCGGTGACTTTTAGTCGAATTAGCTCTGTTAAAATAATAGGTGAATTTGGTCAAGGATATCTCAAATGGGTTTCTCAAAAACATGTTGTGAGGAGTCCCATTTCTGTGAAGTTTAATTAA

Coding sequence (CDS)

ATGACTACTGCAAACATAAAAACTCCTCAAGGAAAACCAATTCTGGATCAAGATTTGAAACCGGCTAACTTTTTTGCAATTGGTGCTGGACATGTAAACCCATCAAAGGCAGCTGACCCAGGATTGGTTTATGACATTCAATCTGATGATTACATTCCTTATCTTTGTGGTTTGTACAAAGATGAAGAAGTTTCAATTATTGTTCATAAAACAGTAATCTGCGGATTAGTACCAAGGATTCGAGAAGGGGATTTGAATTATCCTTCGTTTAGTGTCGAGTTAGGAGGCTTACAAACGTTCAAAAGAACAGTGACAAATGTGGGTGAAGCAAACTCGGTTTATTCTGTCATGGTTGAAGCACCGCTGGGAGTTTCTATGACGGTCTTACCAAGAAAATTGGTGTTCTCGAGGGTGAACCAAAAGTTGACCTTTTCGGTGACTTTTAGTCGAATTAGCTCTGTTAAAATAATAGGTGAATTTGGTCAAGGATATCTCAAATGGGTTTCTCAAAAACATGTTGTGAGGAGTCCCATTTCTGTGAAGTTTAATTAA

Protein sequence

MTTANIKTPQGKPILDQDLKPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLCGLYKDEEVSIIVHKTVICGLVPRIREGDLNYPSFSVELGGLQTFKRTVTNVGEANSVYSVMVEAPLGVSMTVLPRKLVFSRVNQKLTFSVTFSRISSVKIIGEFGQGYLKWVSQKHVVRSPISVKFN

Homology

[Close]

BLAST of Lsi09G003420 vs. Swiss-Prot
Match: SBT12_ARATH (Subtilisin-like protease SBT1.2 OS=Arabidopsis thaliana GN=SBT1.2 PE=2 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 1.4e-34
Identity = 90/190 (47.37%), Postives = 116/190 (61.05%), Query Frame = 1


Query: 1   MTTANIKTPQGKPILDQDLKPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLCGL-Y 60
           MTTA++   QGK I D + KPA  FAIGAGHVNP KA +PGLVY+IQ  DYI YLC L +
Sbjct: 582 MTTADLYDRQGKAIKDGN-KPAGVFAIGAGHVNPQKAINPGLVYNIQPVDYITYLCTLGF 641

Query: 61  KDEEVSIIVHKTVICGLVPRIREG-DLNYPSFSVELGGLQT---FKRTVTNVGEANSVYS 120
              ++  I HK V C  + R   G  LNYPS +V     +T     R VTNVG  NS+YS
Sbjct: 642 TRSDILAITHKNVSCNGILRKNPGFSLNYPSIAVIFKRGKTTEMITRRVTNVGSPNSIYS 701

Query: 121 VMVEAPLGVSMTVLPRKLVFSRVNQKLTFSVTF--SRISSVKIIGEFGQGYLKWVSQKHV 180
           V V+AP G+ + V P++LVF  V+Q L++ V F   + +    +  F QG L WV+  ++
Sbjct: 702 VNVKAPEGIKVIVNPKRLVFKHVDQTLSYRVWFVLKKKNRGGKVASFAQGQLTWVNSHNL 761

[Close]

BLAST of Lsi09G003420 vs. Swiss-Prot
Match: SBT11_ARATH (Subtilisin-like protease SBT1.1 OS=Arabidopsis thaliana GN=SBTI1.1 PE=1 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 1.2e-28
Identity = 78/191 (40.84%), Postives = 107/191 (56.02%), Query Frame = 1


Query: 1   MTTANIKTPQGKPILDQDL----KPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLC 60
           MTTA I   + +PI D+        A  FA GAG+V+P++A DPGLVYD  + DY+ YLC
Sbjct: 582 MTTARITDNRNRPIGDRGAAGAESAATAFAFGAGNVDPTRAVDPGLVYDTSTVDYLNYLC 641

Query: 61  GL-YKDEEVSIIVHKTVICGL-VPRIREGDLNYPSFSVEL---GGLQT--FKRTVTNVGE 120
            L Y  E + +       C      +  GDLNYPSF+V L     L+T  +KRTVTNVG 
Sbjct: 642 SLNYTSERILLFSGTNYTCASNAVVLSPGDLNYPSFAVNLVNGANLKTVRYKRTVTNVGS 701

Query: 121 ANSVYSVMVEAPLGVSMTVLPRKLVFSRVNQKLTFSVTFSRISSVKIIGEFGQGYLKWVS 180
               Y V VE P GV + V P+ L F +  ++L+++VT+   +S +       G L W+ 
Sbjct: 702 PTCEYMVHVEEPKGVKVRVEPKVLKFQKARERLSYTVTYDAEAS-RNSSSSSFGVLVWIC 761

[Close]

BLAST of Lsi09G003420 vs. Swiss-Prot
Match: SBT18_ARATH (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana GN=SBT1.8 PE=2 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 2.0e-28
Identity = 74/187 (39.57%), Postives = 104/187 (55.61%), Query Frame = 1


Query: 1   MTTANIKTPQGKPILDQ-DLKPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLCGL- 60
           MTTA +      P+ D  D   +N +A G+GHV+P KA  PGLVYDI +++YI +LC L 
Sbjct: 566 MTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLD 625

Query: 61  YKDEEVSIIVHKTVICGLVPRIREGDLNYPSFSVELGGLQT--FKRTVTNVGEANSVYSV 120
           Y  + +  IV +  +         G LNYPSFSV  GG +   + R VTNVG A+SVY V
Sbjct: 626 YTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSVLFGGKRVVRYTREVTNVGAASSVYKV 685

Query: 121 MVEAPLGVSMTVLPRKLVFSRVNQKLTFSVTFSRISSVKIIGEFGQGYLKWVSQKHVVRS 180
            V     V ++V P KL F  V +K  ++VTF     V +  +   G + W + +H VRS
Sbjct: 686 TVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFGSITWSNPQHEVRS 745

Query: 181 PISVKFN 184
           P++  +N
Sbjct: 746 PVAFSWN 752

[Close]

BLAST of Lsi09G003420 vs. Swiss-Prot
Match: SBT17_ARATH (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 2.0e-28
Identity = 74/187 (39.57%), Postives = 110/187 (58.82%), Query Frame = 1


Query: 1   MTTANIKTPQGKPILDQDL-KPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLCGL- 60
           MTTA      GKP+LD    KP+  F  GAGHV+P+ A +PGL+YD+ ++DY+ +LC L 
Sbjct: 572 MTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALN 631

Query: 61  YKDEEVSIIVHKTVICGLVPRIREGDLNYPSFSVELGGLQTFK--RTVTNVGEANSVYSV 120
           Y   ++  +  +   C         DLNYPSF+V + G+  +K  RTVT+VG A + YSV
Sbjct: 632 YTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGT-YSV 691

Query: 121 MVEA-PLGVSMTVLPRKLVFSRVNQKLTFSVTFSRISSVKIIGEFGQGYLKWVSQKHVVR 180
            V +   GV ++V P  L F   N+K +++VTF+ + S K  G    G ++W   KHVV 
Sbjct: 692 KVTSETTGVKISVEPAVLNFKEANEKKSYTVTFT-VDSSKPSGSNSFGSIEWSDGKHVVG 751

Query: 181 SPISVKF 183
           SP+++ +
Sbjct: 752 SPVAISW 756

[Close]

BLAST of Lsi09G003420 vs. Swiss-Prot
Match: SBT16_ARATH (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 5.7e-28
Identity = 74/191 (38.74%), Postives = 111/191 (58.12%), Query Frame = 1


Query: 1   MTTANIKTPQGKPILDQDL-KPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLCGL- 60
           MTT N+     + ++D+   K A  +  G+GH+N  +A +PGLVYDI +DDYI +LC + 
Sbjct: 568 MTTTNLVDNSNRSLIDESTGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIG 627

Query: 61  YKDEEVSIIVHKTVICGLVPRIREGDLNYPS----FSVELGGL--QTFKRTVTNVGEANS 120
           Y  + + +I    V C    +   G+LNYPS    F     GL  +T  RT TNVG+A +
Sbjct: 628 YGPKTIQVITRTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEA 687

Query: 121 VYSVMVEAPLGVSMTVLPRKLVFSRVNQKLTFSVTFSRISSVKIIGEFGQ--GYLKWV-S 180
           VY   +E+P GV++TV P +LVF+   ++ +++VT +  +   ++GE G   G + W   
Sbjct: 688 VYRARIESPRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDG 747

[Close]

BLAST of Lsi09G003420 vs. TrEMBL
Match: A0A0A0KMQ7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G157280 PE=4 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 6.8e-68
Identity = 127/182 (69.78%), Postives = 150/182 (82.42%), Query Frame = 1


Query: 1   MTTANIKTPQGKPILDQDLKPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLCGLYK 60
           MT+A+++ PQGKPI+DQDLKPANFFA+G+GHVNPSKAA+PGLVYDIQ DDY+PYLC LY 
Sbjct: 551 MTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYVPYLCHLYT 610

Query: 61  DEEVSIIVHKTVICGLVPRIREGDLNYPSFSVELGG-LQTFKRTVTNVGEANSVYSVMVE 120
           D +VSIIV + V C  V RIREGDLNYPSF+V LG   Q F RTVTNVG+ANSVY  +V+
Sbjct: 611 DAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVGDANSVYYAIVK 670

Query: 121 APLGVSMTVLPRKLVFSRVNQKLTFSVTFSRISSVKIIGEFGQGYLKWVSQKHVVRSPIS 180
           AP GVS+ V PR L FS++N+KLT+SVTFSRI  V+   EF +GYL WVS KH+VRSPIS
Sbjct: 671 APAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIWVSNKHIVRSPIS 730

Query: 181 VK 182
           VK
Sbjct: 731 VK 732

[Close]

BLAST of Lsi09G003420 vs. TrEMBL
Match: A0A0A0KKE3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G157240 PE=4 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 3.8e-63
Identity = 121/183 (66.12%), Postives = 143/183 (78.14%), Query Frame = 1


Query: 1   MTTANIKTPQGKPILDQDLKPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLCGL-Y 60
           MTTANI   +G PI+DQ L+PA+ FAIGAGHVNPSKA DPGLVYDIQ DDYIPYLCGL Y
Sbjct: 562 MTTANITNLEGNPIVDQTLQPADLFAIGAGHVNPSKAVDPGLVYDIQPDDYIPYLCGLGY 621

Query: 61  KDEEVSIIVHKTVICGLVPRIREGDLNYPSFSVELGGLQTFKRTVTNVGEANSVYSVMVE 120
            + +VS+I HK + C     I EG+LNYPSF V+LG +QTF RTVT VG    VY+V++E
Sbjct: 622 TNNQVSLIAHKPIDCLTTTSIPEGELNYPSFMVKLGQVQTFSRTVTYVGSGREVYNVVIE 681

Query: 121 APLGVSMTVLPRKLVFSRVNQKLTFSVTFSRISSVKIIGEFGQGYLKWVSQKHVVRSPIS 180
           AP GVS+TV PRK++FS +NQK T+SVTF RI S+    EF +GYLKWVS KH+VRSPIS
Sbjct: 682 APEGVSVTVRPRKVIFSALNQKATYSVTFKRIGSISPSTEFAEGYLKWVSAKHLVRSPIS 741

Query: 181 VKF 183
           VKF
Sbjct: 742 VKF 744

[Close]

BLAST of Lsi09G003420 vs. TrEMBL
Match: M5WE04_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa002068mg PE=4 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 2.0e-59
Identity = 120/182 (65.93%), Postives = 141/182 (77.47%), Query Frame = 1


Query: 1   MTTANIKTPQGKPILDQDLKPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLCGL-Y 60
           MT+A++   +GKPI D+ L+PA+  A G GHVNPSKA DPGLVYDIQ DDYIPYLCGL Y
Sbjct: 538 MTSADLLNLEGKPIPDETLQPADVLATGVGHVNPSKANDPGLVYDIQPDDYIPYLCGLGY 597

Query: 61  KDEEVSIIVHKTVICGLVPRIREGDLNYPSFSVELGGLQTFKRTVTNVGEANSVYSVMVE 120
           KD+EVSI+VH+ + C  V  I EG+LNYPSFSV LG  QTF RTVTNVGEA S Y+V V 
Sbjct: 598 KDDEVSILVHRPIKCSKVSSIPEGELNYPSFSVALGPSQTFTRTVTNVGEAYSSYAVKVN 657

Query: 121 APLGVSMTVLPRKLVFSRVNQKLTFSVTFSRISSVKIIGEFGQGYLKWVSQKHVVRSPIS 180
           AP  V +++ P+ L FS+VNQKL++SVTFSRI S    GEF QG+L WVS KHVVRSPIS
Sbjct: 658 APEEVYVSINPKTLYFSKVNQKLSYSVTFSRIGSRGKAGEFTQGFLTWVSAKHVVRSPIS 717

Query: 181 VK 182
           VK
Sbjct: 718 VK 719

[Close]

BLAST of Lsi09G003420 vs. TrEMBL
Match: A0A067JNI9_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_20707 PE=4 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 4.9e-58
Identity = 115/183 (62.84%), Postives = 138/183 (75.41%), Query Frame = 1


Query: 1   MTTANIKTPQGKPILDQDLKPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLCGL-Y 60
           MTTA+I   +G PI+D+  +PA+ F IGAGHVNPS+A DPGL+YDIQ DDYIPYLCGL Y
Sbjct: 574 MTTADIFNMEGSPIVDEKHQPADLFTIGAGHVNPSRANDPGLIYDIQPDDYIPYLCGLGY 633

Query: 61  KDEEVSIIVHKTVICGLVPRIREGDLNYPSFSVELGGLQTFKRTVTNVGEANSVYSVMVE 120
           K+E+VSII H+ + C     I EG LNYPSFSV LG  QTF RTVTNVGEANSVY+  + 
Sbjct: 634 KEEQVSIIAHRRIKCSEKLSIPEGQLNYPSFSVTLGASQTFTRTVTNVGEANSVYAATIV 693

Query: 121 APLGVSMTVLPRKLVFSRVNQKLTFSVTFSRISSVKIIGEFGQGYLKWVSQKHVVRSPIS 180
            P GV++TV P +L FS+VNQK+T+SVTFS   S     EF QGY+ W S KH+VRSPIS
Sbjct: 694 PPPGVAVTVEPYRLYFSQVNQKVTYSVTFSPTGSSGKTSEFAQGYILWSSAKHLVRSPIS 753

Query: 181 VKF 183
           V+F
Sbjct: 754 VRF 756

[Close]

BLAST of Lsi09G003420 vs. TrEMBL
Match: W9RYM8_9ROSA (Subtilisin-like protease SDD1 OS=Morus notabilis GN=L484_023157 PE=4 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 1.1e-57
Identity = 116/183 (63.39%), Postives = 136/183 (74.32%), Query Frame = 1


Query: 1   MTTANIKTPQGKPILDQDLKPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLCGL-Y 60
           MTTA+I   +GK ILDQ L PA+ FA GAGHVNP KA DPGL+YD+Q DDYIPYLCGL Y
Sbjct: 656 MTTADIVNLEGKAILDQALTPADVFATGAGHVNPIKANDPGLIYDLQPDDYIPYLCGLGY 715

Query: 61  KDEEVSIIVHKTVICGLVPRIREGDLNYPSFSVELGGLQTFKRTVTNVGEANSVYSVMVE 120
            D+EV I+  + + C   P I EG+LNYPSFSV LG  QTF RTVTNVGEA S Y+  + 
Sbjct: 716 NDKEVGIVARRPIKCSEKPSIPEGELNYPSFSVTLGPSQTFTRTVTNVGEAYSTYTANIM 775

Query: 121 APLGVSMTVLPRKLVFSRVNQKLTFSVTFSRISSVKIIGEFGQGYLKWVSQKHVVRSPIS 180
           AP GV ++V P KL FS+VNQK T+SV FSRI+S    G +GQG+L WVS +H VRSPIS
Sbjct: 776 APDGVYVSVKPSKLYFSKVNQKATYSVNFSRITSSGETGPYGQGFLTWVSARHCVRSPIS 835

Query: 181 VKF 183
           VKF
Sbjct: 836 VKF 838

[Close]

BLAST of Lsi09G003420 vs. TAIR10
Match: AT1G04110.1 (AT1G04110.1 Subtilase family protein)

HSP 1 Score: 147.5 bits (371), Expect = 8.0e-36
Identity = 90/190 (47.37%), Postives = 116/190 (61.05%), Query Frame = 1


Query: 1   MTTANIKTPQGKPILDQDLKPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLCGL-Y 60
           MTTA++   QGK I D + KPA  FAIGAGHVNP KA +PGLVY+IQ  DYI YLC L +
Sbjct: 582 MTTADLYDRQGKAIKDGN-KPAGVFAIGAGHVNPQKAINPGLVYNIQPVDYITYLCTLGF 641

Query: 61  KDEEVSIIVHKTVICGLVPRIREG-DLNYPSFSVELGGLQT---FKRTVTNVGEANSVYS 120
              ++  I HK V C  + R   G  LNYPS +V     +T     R VTNVG  NS+YS
Sbjct: 642 TRSDILAITHKNVSCNGILRKNPGFSLNYPSIAVIFKRGKTTEMITRRVTNVGSPNSIYS 701

Query: 121 VMVEAPLGVSMTVLPRKLVFSRVNQKLTFSVTF--SRISSVKIIGEFGQGYLKWVSQKHV 180
           V V+AP G+ + V P++LVF  V+Q L++ V F   + +    +  F QG L WV+  ++
Sbjct: 702 VNVKAPEGIKVIVNPKRLVFKHVDQTLSYRVWFVLKKKNRGGKVASFAQGQLTWVNSHNL 761

[Close]

BLAST of Lsi09G003420 vs. TAIR10
Match: AT1G01900.1 (AT1G01900.1 subtilase family protein)

HSP 1 Score: 127.9 bits (320), Expect = 6.5e-30
Identity = 78/191 (40.84%), Postives = 107/191 (56.02%), Query Frame = 1


Query: 1   MTTANIKTPQGKPILDQDL----KPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLC 60
           MTTA I   + +PI D+        A  FA GAG+V+P++A DPGLVYD  + DY+ YLC
Sbjct: 582 MTTARITDNRNRPIGDRGAAGAESAATAFAFGAGNVDPTRAVDPGLVYDTSTVDYLNYLC 641

Query: 61  GL-YKDEEVSIIVHKTVICGL-VPRIREGDLNYPSFSVEL---GGLQT--FKRTVTNVGE 120
            L Y  E + +       C      +  GDLNYPSF+V L     L+T  +KRTVTNVG 
Sbjct: 642 SLNYTSERILLFSGTNYTCASNAVVLSPGDLNYPSFAVNLVNGANLKTVRYKRTVTNVGS 701

Query: 121 ANSVYSVMVEAPLGVSMTVLPRKLVFSRVNQKLTFSVTFSRISSVKIIGEFGQGYLKWVS 180
               Y V VE P GV + V P+ L F +  ++L+++VT+   +S +       G L W+ 
Sbjct: 702 PTCEYMVHVEEPKGVKVRVEPKVLKFQKARERLSYTVTYDAEAS-RNSSSSSFGVLVWIC 761

[Close]

BLAST of Lsi09G003420 vs. TAIR10
Match: AT2G05920.1 (AT2G05920.1 Subtilase family protein)

HSP 1 Score: 127.1 bits (318), Expect = 1.1e-29
Identity = 74/187 (39.57%), Postives = 104/187 (55.61%), Query Frame = 1


Query: 1   MTTANIKTPQGKPILDQ-DLKPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLCGL- 60
           MTTA +      P+ D  D   +N +A G+GHV+P KA  PGLVYDI +++YI +LC L 
Sbjct: 566 MTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLD 625

Query: 61  YKDEEVSIIVHKTVICGLVPRIREGDLNYPSFSVELGGLQT--FKRTVTNVGEANSVYSV 120
           Y  + +  IV +  +         G LNYPSFSV  GG +   + R VTNVG A+SVY V
Sbjct: 626 YTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSVLFGGKRVVRYTREVTNVGAASSVYKV 685

Query: 121 MVEAPLGVSMTVLPRKLVFSRVNQKLTFSVTFSRISSVKIIGEFGQGYLKWVSQKHVVRS 180
            V     V ++V P KL F  V +K  ++VTF     V +  +   G + W + +H VRS
Sbjct: 686 TVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFGSITWSNPQHEVRS 745

Query: 181 PISVKFN 184
           P++  +N
Sbjct: 746 PVAFSWN 752

[Close]

BLAST of Lsi09G003420 vs. TAIR10
Match: AT5G67360.1 (AT5G67360.1 Subtilase family protein)

HSP 1 Score: 127.1 bits (318), Expect = 1.1e-29
Identity = 74/187 (39.57%), Postives = 110/187 (58.82%), Query Frame = 1


Query: 1   MTTANIKTPQGKPILDQDL-KPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLCGL- 60
           MTTA      GKP+LD    KP+  F  GAGHV+P+ A +PGL+YD+ ++DY+ +LC L 
Sbjct: 572 MTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALN 631

Query: 61  YKDEEVSIIVHKTVICGLVPRIREGDLNYPSFSVELGGLQTFK--RTVTNVGEANSVYSV 120
           Y   ++  +  +   C         DLNYPSF+V + G+  +K  RTVT+VG A + YSV
Sbjct: 632 YTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGT-YSV 691

Query: 121 MVEA-PLGVSMTVLPRKLVFSRVNQKLTFSVTFSRISSVKIIGEFGQGYLKWVSQKHVVR 180
            V +   GV ++V P  L F   N+K +++VTF+ + S K  G    G ++W   KHVV 
Sbjct: 692 KVTSETTGVKISVEPAVLNFKEANEKKSYTVTFT-VDSSKPSGSNSFGSIEWSDGKHVVG 751

Query: 181 SPISVKF 183
           SP+++ +
Sbjct: 752 SPVAISW 756

[Close]

BLAST of Lsi09G003420 vs. TAIR10
Match: AT4G34980.1 (AT4G34980.1 subtilisin-like serine protease 2)

HSP 1 Score: 125.6 bits (314), Expect = 3.2e-29
Identity = 74/191 (38.74%), Postives = 111/191 (58.12%), Query Frame = 1


Query: 1   MTTANIKTPQGKPILDQDL-KPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLCGL- 60
           MTT N+     + ++D+   K A  +  G+GH+N  +A +PGLVYDI +DDYI +LC + 
Sbjct: 568 MTTTNLVDNSNRSLIDESTGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIG 627

Query: 61  YKDEEVSIIVHKTVICGLVPRIREGDLNYPS----FSVELGGL--QTFKRTVTNVGEANS 120
           Y  + + +I    V C    +   G+LNYPS    F     GL  +T  RT TNVG+A +
Sbjct: 628 YGPKTIQVITRTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEA 687

Query: 121 VYSVMVEAPLGVSMTVLPRKLVFSRVNQKLTFSVTFSRISSVKIIGEFGQ--GYLKWV-S 180
           VY   +E+P GV++TV P +LVF+   ++ +++VT +  +   ++GE G   G + W   
Sbjct: 688 VYRARIESPRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDG 747

[Close]

BLAST of Lsi09G003420 vs. NCBI nr
Match: gi|659074655|ref|XP_008437722.1| (PREDICTED: subtilisin-like protease [Cucumis melo])

HSP 1 Score: 322.8 bits (826), Expect = 3.9e-85
Identity = 157/184 (85.33%), Postives = 172/184 (93.48%), Query Frame = 1


Query: 1   MTTANIKTPQGKPILDQDLKPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLCGLYK 60
           MTTANIKTPQG+PI DQDL+PANFFA+GAGHVNPSKAADPGLVYDIQ DDYIPYLCGLYK
Sbjct: 580 MTTANIKTPQGEPITDQDLQPANFFAMGAGHVNPSKAADPGLVYDIQPDDYIPYLCGLYK 639

Query: 61  DEEVSIIVHKTVICGLVPRIREGDLNYPSFSVELGGLQTFKRTVTNVGEANSVYSVMVEA 120
           DEEVSIIVH+TVICGLV  IREGDLNYPSFSV LGGLQTFKRTVTNVGEANSVY+ +VEA
Sbjct: 640 DEEVSIIVHRTVICGLVLSIREGDLNYPSFSVALGGLQTFKRTVTNVGEANSVYTAIVEA 699

Query: 121 PLGVSMTVLPRKLVFSRVNQKLTFSVTFSRISSVKIIGEFGQGYLKWV--SQKHVVRSPI 180
           PLGVSMTV+PRKL FSRVNQ +TF+VTF+RI  VKI+GEFG+GYLKWV  S+K+VVRSP+
Sbjct: 700 PLGVSMTVMPRKLFFSRVNQTMTFTVTFNRIRWVKIVGEFGEGYLKWVSKSKKYVVRSPV 759

Query: 181 SVKF 183
           SVKF
Sbjct: 760 SVKF 763

[Close]

BLAST of Lsi09G003420 vs. NCBI nr
Match: gi|659073650|ref|XP_008437177.1| (PREDICTED: subtilisin-like protease [Cucumis melo])

HSP 1 Score: 267.7 bits (683), Expect = 1.5e-68
Identity = 130/182 (71.43%), Postives = 152/182 (83.52%), Query Frame = 1


Query: 1   MTTANIKTPQGKPILDQDLKPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLCGLYK 60
           MT+A+++ PQGKPI+DQDLKPANFFA+G+GHVNPSKAA+PGLVYDIQ DDYIPYLC LY 
Sbjct: 571 MTSADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYIPYLCHLYT 630

Query: 61  DEEVSIIVHKTVICGLVPRIREGDLNYPSFSVELG-GLQTFKRTVTNVGEANSVYSVMVE 120
           D +VSIIV K V C  VPRIREGDLNYPSF+V LG G QTF RTVTNVG+ANSVY  +VE
Sbjct: 631 DAQVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLGAGSQTFNRTVTNVGDANSVYYPIVE 690

Query: 121 APLGVSMTVLPRKLVFSRVNQKLTFSVTFSRISSVKIIGEFGQGYLKWVSQKHVVRSPIS 180
           AP GVS+ V P  L FS++N+K+T+SVTFSRI  V+   EF +GYL WVS+K +VRSPIS
Sbjct: 691 APAGVSVKVTPSNLKFSKLNEKVTYSVTFSRIDFVRTTSEFSEGYLIWVSKKLMVRSPIS 750

Query: 181 VK 182
           VK
Sbjct: 751 VK 752

[Close]

BLAST of Lsi09G003420 vs. NCBI nr
Match: gi|449459730|ref|XP_004147599.1| (PREDICTED: subtilisin-like protease SBT1.7 [Cucumis sativus])

HSP 1 Score: 265.0 bits (676), Expect = 9.7e-68
Identity = 127/182 (69.78%), Postives = 150/182 (82.42%), Query Frame = 1


Query: 1   MTTANIKTPQGKPILDQDLKPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLCGLYK 60
           MT+A+++ PQGKPI+DQDLKPANFFA+G+GHVNPSKAA+PGLVYDIQ DDY+PYLC LY 
Sbjct: 551 MTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYVPYLCHLYT 610

Query: 61  DEEVSIIVHKTVICGLVPRIREGDLNYPSFSVELGG-LQTFKRTVTNVGEANSVYSVMVE 120
           D +VSIIV + V C  V RIREGDLNYPSF+V LG   Q F RTVTNVG+ANSVY  +V+
Sbjct: 611 DAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVGDANSVYYAIVK 670

Query: 121 APLGVSMTVLPRKLVFSRVNQKLTFSVTFSRISSVKIIGEFGQGYLKWVSQKHVVRSPIS 180
           AP GVS+ V PR L FS++N+KLT+SVTFSRI  V+   EF +GYL WVS KH+VRSPIS
Sbjct: 671 APAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIWVSNKHIVRSPIS 730

Query: 181 VK 182
           VK
Sbjct: 731 VK 732

[Close]

BLAST of Lsi09G003420 vs. NCBI nr
Match: gi|449459724|ref|XP_004147596.1| (PREDICTED: subtilisin-like protease SBT1.2 [Cucumis sativus])

HSP 1 Score: 249.2 bits (635), Expect = 5.5e-63
Identity = 121/183 (66.12%), Postives = 143/183 (78.14%), Query Frame = 1


Query: 1   MTTANIKTPQGKPILDQDLKPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLCGL-Y 60
           MTTANI   +G PI+DQ L+PA+ FAIGAGHVNPSKA DPGLVYDIQ DDYIPYLCGL Y
Sbjct: 562 MTTANITNLEGNPIVDQTLQPADLFAIGAGHVNPSKAVDPGLVYDIQPDDYIPYLCGLGY 621

Query: 61  KDEEVSIIVHKTVICGLVPRIREGDLNYPSFSVELGGLQTFKRTVTNVGEANSVYSVMVE 120
            + +VS+I HK + C     I EG+LNYPSF V+LG +QTF RTVT VG    VY+V++E
Sbjct: 622 TNNQVSLIAHKPIDCLTTTSIPEGELNYPSFMVKLGQVQTFSRTVTYVGSGREVYNVVIE 681

Query: 121 APLGVSMTVLPRKLVFSRVNQKLTFSVTFSRISSVKIIGEFGQGYLKWVSQKHVVRSPIS 180
           AP GVS+TV PRK++FS +NQK T+SVTF RI S+    EF +GYLKWVS KH+VRSPIS
Sbjct: 682 APEGVSVTVRPRKVIFSALNQKATYSVTFKRIGSISPSTEFAEGYLKWVSAKHLVRSPIS 741

Query: 181 VKF 183
           VKF
Sbjct: 742 VKF 744

[Close]

BLAST of Lsi09G003420 vs. NCBI nr
Match: gi|659073656|ref|XP_008437181.1| (PREDICTED: subtilisin-like protease [Cucumis melo])

HSP 1 Score: 246.5 bits (628), Expect = 3.6e-62
Identity = 122/183 (66.67%), Postives = 142/183 (77.60%), Query Frame = 1


Query: 1   MTTANIKTPQGKPILDQDLKPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLCGL-Y 60
           MTTANI   +G PILD+ L+PA+ FAIGAGHVNPSKA DPGLVYDIQ DDYIPYLCGL Y
Sbjct: 562 MTTANITNLEGNPILDETLQPADLFAIGAGHVNPSKAIDPGLVYDIQPDDYIPYLCGLGY 621

Query: 61  KDEEVSIIVHKTVICGLVPRIREGDLNYPSFSVELGGLQTFKRTVTNVGEANSVYSVMVE 120
            + +VS+I HK + C     I EG+LNYPSF V+LG +QTF RTVT+VG    VY+V++E
Sbjct: 622 TNNQVSLIAHKPIDCLTTSSIPEGELNYPSFMVKLGPVQTFSRTVTSVGSGRVVYNVVIE 681

Query: 121 APLGVSMTVLPRKLVFSRVNQKLTFSVTFSRISSVKIIGEFGQGYLKWVSQKHVVRSPIS 180
           AP GVS+TV PRKL FS +NQK T+SVTF R  S+    EF +GYLKWVS KHVVRSPIS
Sbjct: 682 APEGVSVTVRPRKLSFSALNQKATYSVTFKRSGSISPSIEFAEGYLKWVSAKHVVRSPIS 741

Query: 181 VKF 183
           VKF
Sbjct: 742 VKF 744

The following BLAST results are available for this feature:

BLAST of Lsi09G003420 vs. Swiss-Prot


Match Name	E-value	Identity	Description
SBT12_ARATH	1.4e-34	47.37	Subtilisin-like protease SBT1.2 OS=Arabidopsis thaliana GN=SBT1.2 PE=2 SV=1	[more]
SBT11_ARATH	1.2e-28	40.84	Subtilisin-like protease SBT1.1 OS=Arabidopsis thaliana GN=SBTI1.1 PE=1 SV=1	[more]
SBT18_ARATH	2.0e-28	39.57	Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana GN=SBT1.8 PE=2 SV=1	[more]
SBT17_ARATH	2.0e-28	39.57	Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana GN=SBT1.7 PE=1 SV=1	[more]
SBT16_ARATH	5.7e-28	38.74	Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana GN=SBT1.6 PE=2 SV=1	[more]

BLAST of Lsi09G003420 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KMQ7_CUCSA	6.8e-68	69.78	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G157280 PE=4 SV=1	[more]
A0A0A0KKE3_CUCSA	3.8e-63	66.12	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G157240 PE=4 SV=1	[more]
M5WE04_PRUPE	2.0e-59	65.93	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa002068mg PE=4 SV=1	[more]
A0A067JNI9_JATCU	4.9e-58	62.84	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_20707 PE=4 SV=1	[more]
W9RYM8_9ROSA	1.1e-57	63.39	Subtilisin-like protease SDD1 OS=Morus notabilis GN=L484_023157 PE=4 SV=1	[more]

BLAST of Lsi09G003420 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G04110.1	8.0e-36	47.37	Subtilase family protein	[more]
AT1G01900.1	6.5e-30	40.84	subtilase family protein	[more]
AT2G05920.1	1.1e-29	39.57	Subtilase family protein	[more]
AT5G67360.1	1.1e-29	39.57	Subtilase family protein	[more]
AT4G34980.1	3.2e-29	38.74	subtilisin-like serine protease 2	[more]

BLAST of Lsi09G003420 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659074655\|ref\|XP_008437722.1\|	3.9e-85	85.33	PREDICTED: subtilisin-like protease [Cucumis melo]	[more]
gi\|659073650\|ref\|XP_008437177.1\|	1.5e-68	71.43	PREDICTED: subtilisin-like protease [Cucumis melo]	[more]
gi\|449459730\|ref\|XP_004147599.1\|	9.7e-68	69.78	PREDICTED: subtilisin-like protease SBT1.7 [Cucumis sativus]	[more]
gi\|449459724\|ref\|XP_004147596.1\|	5.5e-63	66.12	PREDICTED: subtilisin-like protease SBT1.2 [Cucumis sativus]	[more]
gi\|659073656\|ref\|XP_008437181.1\|	3.6e-62	66.67	PREDICTED: subtilisin-like protease [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR015500	Peptidase_S8_subtilisin-rel

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005618	cell wall
cellular_component	GO:0016020	membrane
molecular_function	GO:0004252	serine-type endopeptidase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi09G003420.1	Lsi09G003420.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015500	Peptidase S8, subtilisin-related	PANTHER	PTHR10795	PROPROTEIN CONVERTASE SUBTILISIN/KEXIN	coord: 1..182 score: 6.6
None	No IPR available	PANTHER	PTHR10795:SF370	SUBFAMILY NOT NAMED	coord: 1..182 score: 6.6

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi09G003420	Bottle gourd (USVL1VR-Ls)	lsilsiB008
Lsi09G003420	Cucumber (Gy14) v1	cgylsiB411
Lsi09G003420	Cucumber (Gy14) v1	cgylsiB487
Lsi09G003420	Cucurbita maxima (Rimu)	cmalsiB267
Lsi09G003420	Cucurbita maxima (Rimu)	cmalsiB365
Lsi09G003420	Cucurbita maxima (Rimu)	cmalsiB530
Lsi09G003420	Cucurbita moschata (Rifu)	cmolsiB255
Lsi09G003420	Cucurbita moschata (Rifu)	cmolsiB353
Lsi09G003420	Cucurbita moschata (Rifu)	cmolsiB516
Lsi09G003420	Cucurbita pepo (Zucchini)	cpelsiB131
Lsi09G003420	Wild cucumber (PI 183967)	cpilsiB155
Lsi09G003420	Wild cucumber (PI 183967)	cpilsiB322
Lsi09G003420	Cucumber (Chinese Long) v2	culsiB156
Lsi09G003420	Cucumber (Chinese Long) v2	culsiB322
Lsi09G003420	Melon (DHL92) v3.5.1	lsimeB012
Lsi09G003420	Melon (DHL92) v3.5.1	lsimeB036
Lsi09G003420	Watermelon (Charleston Gray)	lsiwcgB008
Lsi09G003420	Watermelon (Charleston Gray)	lsiwcgB023
Lsi09G003420	Watermelon (97103) v1	lsiwmB024
Lsi09G003420	Watermelon (97103) v1	lsiwmB027
Lsi09G003420	Cucumber (Gy14) v2	cgyblsiB141
Lsi09G003420	Cucumber (Gy14) v2	cgyblsiB294
Lsi09G003420	Melon (DHL92) v3.6.1	lsimedB012
Lsi09G003420	Melon (DHL92) v3.6.1	lsimedB036
Lsi09G003420	Silver-seed gourd	carlsiB851
Lsi09G003420	Cucumber (Chinese Long) v3	cuclsiB171
Lsi09G003420	Cucumber (Chinese Long) v3	cuclsiB345
Lsi09G003420	Watermelon (97103) v2	lsiwmbB007
Lsi09G003420	Wax gourd	lsiwgoB010