Lsi09G003410 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi09G003410
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionSubtilisin-like serine protease
Locationchr09 : 3541767 .. 3543381 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGGTTCTTCCTTTCCTCTGTATTTTTTTCTCGCTGAACATTGAGAGCAATGTTGCCGCAGAAATTGAGCTTCCAACTACTCCCCACCAAAGCAAACTTCAAACCTATATTGTCCATGTGGAGCAGCCCAAGATCAGACTTGTAGGTGAATCCTCAAATGATCATATTGAAAGTTGGTACATGTCATTTCTACCGGCAATGACGGAGACTGTGGCCGAGCCGCCGCGTTTGCTTTATGCGTATCGAAATGTAATGAGTGGCTTCTCTGCAAGACTTACAAAAGAACAAGTAAAATCTATGGAGAAGAAGGATGGTTTCATATCAGCAATGCCTGAAACTATAATGAATTTGCACACAACTCATACCCCTGACTATTTGGGGTTGAACCAACAATTTGGATTATGGAAAAATTCAAACTTTGGAAAAGGAGTGATTATAGGAGTGTTGGATACTGGAATTCATCCGAGTCATCCTTCGTTTAGCGACGAAGGAATGTCGCCGCCACCCGCAAAATGGAAAGGAAGATGCGAGTTTAATATCTCTGTATGCAACAACAAATTGATTGGTGCTAGAAATTTCAACCGTGGAAATAGTTTTTTGATGGGGGATCAGTCGCCTTCTGATGAAAAAGGCCATGGCACACACACAGCAAGCACGGCTGCTGGTGCCTTTGTGGAAGGTGCTAAAGCTTTGGGAAATGCTAGAGGCAAGGCGGCTGGCATGGCACCTTTAGCTCACCGTGCAATTTATAAAGTTTGTTCCGTTAAAAGTTGTCCGAGTAGTGACGTTCTTGCAGGGATAGATGCTGCTATCGACGATGGGGTCGACGTGCTATCGATCTCACTCGGGTCTCGCTCGGTTCCCTTTTTCAAGGACAACATAGCTGTAGCCACATTTGGAGCAATTCAAAAGGGGGTTTTTGTTAGTTGTTCTGCAGGAAATTCAGGGCCTTTTAATAGTACATTATCTAATGAAGCGCCATGGATTTTAACAGTTGAAGCAAGCACTATTAATAGAAGGATTGTAGCAGTTGCCAAGCTTGTAAATGGTGAGGAATATGAAGGTGAATCTCTTTACCAACCAAATGACTTTCCATCTACATTTTTACCACTTGTATATGCTGGAAGCAGAGAAAACAAAAACTCTGCATTTTGTGGTGAAGGTTCACTTGAAAATATGGATGAAAGGAGGAGTTGGGAGAGTTGCAAAAGGATTAGTAGTGAAAAATGCTGGTGGTGCAGCAATGATTCTTATAAATCAACAACAAGATGGATTTAGTACTCTGTCTGAAGCTCATGTTCTTCCAGCAACACATGTCAGCTATGAAGCTGGACTCTTGATCAAATCCTACATAAATTCATCACAAAATCCAACTGCATCAATATCATTTAAAGGAACCATAATTGGAGATAATGATGACTTTTCAGCTCCTGCCATGGCTTCTTTTTCTTCTCGAGGGCCCTGCCTTCCTAGCCCTGGGATCTTAAAACCTGACATAACAGGCCCTGGAGTCAACATTCTCGCAGCTTGGCCATTCCCATTAGACAACAACACAAACACAAAATCAACATTTAACGTTATGTCAGGAACATCAATGTCTTGCCTCATTTAA

mRNA sequence

ATGGTGGTTCTTCCTTTCCTCTGTATTTTTTTCTCGCTGAACATTGAGAGCAATGTTGCCGCAGAAATTGAGCTTCCAACTACTCCCCACCAAAGCAAACTTCAAACCTATATTGTCCATGTGGAGCAGCCCAAGATCAGACTTGTAGGTGAATCCTCAAATGATCATATTGAAAGTTGGTACATGTCATTTCTACCGGCAATGACGGAGACTGTGGCCGAGCCGCCGCGTTTGCTTTATGCGTATCGAAATGTAATGAGTGGCTTCTCTGCAAGACTTACAAAAGAACAAGTAAAATCTATGGAGAAGAAGGATGGTTTCATATCAGCAATGCCTGAAACTATAATGAATTTGCACACAACTCATACCCCTGACTATTTGGGGTTGAACCAACAATTTGGATTATGGAAAAATTCAAACTTTGGAAAAGGAGTGATTATAGGAGTGTTGGATACTGGAATTCATCCGAGTCATCCTTCGTTTAGCGACGAAGGAATGTCGCCGCCACCCGCAAAATGGAAAGGAAGATGCGAGTTTAATATCTCTGTATGCAACAACAAATTGATTGGTGCTAGAAATTTCAACCGTGGAAATAGTTTTTTGATGGGGGATCAGTCGCCTTCTGATGAAAAAGGCCATGGCACACACACAGCAAGCACGGCTGCTGGTGCCTTTGTGGAAGGTGCTAAAGCTTTGGGAAATGCTAGAGGCAAGGCGGCTGGCATGGCACCTTTAGCTCACCGTGCAATTTATAAAGTTTGTTCCGTTAAAAGTTGTCCGAGTAGTGACGTTCTTGCAGGGATAGATGCTGCTATCGACGATGGGGTCGACGTGCTATCGATCTCACTCGGGTCTCGCTCGGTTCCCTTTTTCAAGGACAACATAGCTGTAGCCACATTTGGAGCAATTCAAAAGGGGGTTTTTGTTAGTTGTTCTGCAGGAAATTCAGGGCCTTTTAATAGTACATTATCTAATGAAGCGCCATGGATTTTAACAGTTGAAGCAAGCACTATTAATAGAAGGATTGTAGCAGTTGCCAAGCTTGTAAATGAGAAAACAAAAACTCTGCATTTTGTGGTGAAGGTTCACTTGAAAATATGGATGAAAGGAGGAGTTGGGAGAGTTGCAAAAGGATTAGTAGTGAAAAATGCTGGTGGTGCAGCAATGATTCTTATAAATCAACAACAAGATGGATTTAGTACTCTGTCTGAAGCTCATGTTCTTCCAGCAACACATGTCAGCTATGAAGCTGGACTCTTGATCAAATCCTACATAAATTCATCACAAAATCCAACTGCATCAATATCATTTAAAGGAACCATAATTGGAGATAATGATGACTTTTCAGCTCCTGCCATGGCTTCTTTTTCTTCTCGAGGGCCCTGCCTTCCTAGCCCTGGGATCTTAAAACCTGACATAACAGGCCCTGGAGTCAACATTCTCGCAGCTTGGCCATTCCCATTAGACAACAACACAAACACAAAATCAACATTTAACGTTATGTCAGGAACATCAATGTCTTGCCTCATTTAA

Coding sequence (CDS)

ATGGTGGTTCTTCCTTTCCTCTGTATTTTTTTCTCGCTGAACATTGAGAGCAATGTTGCCGCAGAAATTGAGCTTCCAACTACTCCCCACCAAAGCAAACTTCAAACCTATATTGTCCATGTGGAGCAGCCCAAGATCAGACTTGTAGGTGAATCCTCAAATGATCATATTGAAAGTTGGTACATGTCATTTCTACCGGCAATGACGGAGACTGTGGCCGAGCCGCCGCGTTTGCTTTATGCGTATCGAAATGTAATGAGTGGCTTCTCTGCAAGACTTACAAAAGAACAAGTAAAATCTATGGAGAAGAAGGATGGTTTCATATCAGCAATGCCTGAAACTATAATGAATTTGCACACAACTCATACCCCTGACTATTTGGGGTTGAACCAACAATTTGGATTATGGAAAAATTCAAACTTTGGAAAAGGAGTGATTATAGGAGTGTTGGATACTGGAATTCATCCGAGTCATCCTTCGTTTAGCGACGAAGGAATGTCGCCGCCACCCGCAAAATGGAAAGGAAGATGCGAGTTTAATATCTCTGTATGCAACAACAAATTGATTGGTGCTAGAAATTTCAACCGTGGAAATAGTTTTTTGATGGGGGATCAGTCGCCTTCTGATGAAAAAGGCCATGGCACACACACAGCAAGCACGGCTGCTGGTGCCTTTGTGGAAGGTGCTAAAGCTTTGGGAAATGCTAGAGGCAAGGCGGCTGGCATGGCACCTTTAGCTCACCGTGCAATTTATAAAGTTTGTTCCGTTAAAAGTTGTCCGAGTAGTGACGTTCTTGCAGGGATAGATGCTGCTATCGACGATGGGGTCGACGTGCTATCGATCTCACTCGGGTCTCGCTCGGTTCCCTTTTTCAAGGACAACATAGCTGTAGCCACATTTGGAGCAATTCAAAAGGGGGTTTTTGTTAGTTGTTCTGCAGGAAATTCAGGGCCTTTTAATAGTACATTATCTAATGAAGCGCCATGGATTTTAACAGTTGAAGCAAGCACTATTAATAGAAGGATTGTAGCAGTTGCCAAGCTTGTAAATGAGAAAACAAAAACTCTGCATTTTGTGGTGAAGGTTCACTTGAAAATATGGATGAAAGGAGGAGTTGGGAGAGTTGCAAAAGGATTAGTAGTGAAAAATGCTGGTGGTGCAGCAATGATTCTTATAAATCAACAACAAGATGGATTTAGTACTCTGTCTGAAGCTCATGTTCTTCCAGCAACACATGTCAGCTATGAAGCTGGACTCTTGATCAAATCCTACATAAATTCATCACAAAATCCAACTGCATCAATATCATTTAAAGGAACCATAATTGGAGATAATGATGACTTTTCAGCTCCTGCCATGGCTTCTTTTTCTTCTCGAGGGCCCTGCCTTCCTAGCCCTGGGATCTTAAAACCTGACATAACAGGCCCTGGAGTCAACATTCTCGCAGCTTGGCCATTCCCATTAGACAACAACACAAACACAAAATCAACATTTAACGTTATGTCAGGAACATCAATGTCTTGCCTCATTTAA

Protein sequence

MVVLPFLCIFFSLNIESNVAAEIELPTTPHQSKLQTYIVHVEQPKIRLVGESSNDHIESWYMSFLPAMTETVAEPPRLLYAYRNVMSGFSARLTKEQVKSMEKKDGFISAMPETIMNLHTTHTPDYLGLNQQFGLWKNSNFGKGVIIGVLDTGIHPSHPSFSDEGMSPPPAKWKGRCEFNISVCNNKLIGARNFNRGNSFLMGDQSPSDEKGHGTHTASTAAGAFVEGAKALGNARGKAAGMAPLAHRAIYKVCSVKSCPSSDVLAGIDAAIDDGVDVLSISLGSRSVPFFKDNIAVATFGAIQKGVFVSCSAGNSGPFNSTLSNEAPWILTVEASTINRRIVAVAKLVNEKTKTLHFVVKVHLKIWMKGGVGRVAKGLVVKNAGGAAMILINQQQDGFSTLSEAHVLPATHVSYEAGLLIKSYINSSQNPTASISFKGTIIGDNDDFSAPAMASFSSRGPCLPSPGILKPDITGPGVNILAAWPFPLDNNTNTKSTFNVMSGTSMSCLI
BLAST of Lsi09G003410 vs. Swiss-Prot
Match: SBT17_ARATH (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 2.1e-104
Identity = 237/527 (44.97%), Postives = 312/527 (59.20%), Query Frame = 1

Query: 36  TYIVHVEQPKIRLVGESSNDHIESWYMSFLPAMTETVAEPPRLLYAYRNVMSGFSARLTK 95
           TYIVH+ + ++     SS D   +WY S L     ++++   LLY Y N + GFS RLT+
Sbjct: 31  TYIVHMAKSQM----PSSFDLHSNWYDSSL----RSISDSAELLYTYENAIHGFSTRLTQ 90

Query: 96  EQVKSMEKKDGFISAMPETIMNLHTTHTPDYLGLNQQFG-LWKNSNFGKGVIIGVLDTGI 155
           E+  S+  + G IS +PE    LHTT TP +LGL++    L+  +     V++GVLDTG+
Sbjct: 91  EEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYSDVVVGVLDTGV 150

Query: 156 HPSHPSFSDEGMSPPPAKWKGRCE----FNISVCNNKLIGARNFNRGNSFLMG------- 215
            P   S+SDEG  P P+ WKG CE    F  S+CN KLIGAR F RG    MG       
Sbjct: 151 WPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGPIDESKE 210

Query: 216 DQSPSDEKGHGTHTASTAAGAFVEGAKALGNARGKAAGMAPLAHRAIYKVCSVKSCPSSD 275
            +SP D+ GHGTHT+STAAG+ VEGA  LG A G A GMAP A  A+YKVC +  C SSD
Sbjct: 211 SRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWLGGCFSSD 270

Query: 276 VLAGIDAAIDDGVDVLSISLGSRSVPFFKDNIAVATFGAIQKGVFVSCSAGNSGPFNSTL 335
           +LA ID AI D V+VLS+SLG     +++D +A+  F A+++G+ VSCSAGN+GP +S+L
Sbjct: 271 ILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSL 330

Query: 336 SNEAPWILTVEASTINRRIVAVAKLVNEKT--------------KTLHFVV--------- 395
           SN APWI TV A T++R   A+A L N K               K L F+          
Sbjct: 331 SNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFIYAGNASNATN 390

Query: 396 ------------KVHLKIWM--KGGVGRVAKGLVVKNAGGAAMILINQQQDGFSTLSEAH 455
                       KV  KI M  +G   RV KG VVK AGG  MIL N   +G   +++AH
Sbjct: 391 GNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAH 450

Query: 456 VLPATHVSYEAGLLIKSYINSSQNPTASISFKGTIIGDNDDFSAPAMASFSSRGPCLPSP 509
           +LPAT V  +AG +I+ Y+ +  NPTASIS  GT++G      +P +A+FSSRGP   +P
Sbjct: 451 LLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVK---PSPVVAAFSSRGPNSITP 510

BLAST of Lsi09G003410 vs. Swiss-Prot
Match: SBT14_ARATH (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 8.1e-96
Identity = 230/559 (41.14%), Postives = 306/559 (54.74%), Query Frame = 1

Query: 3   VLPFLCIFFSLNIESNVAAEIELPTTPHQSKLQTYIVHVEQPKIRLVGESSNDHIESWYM 62
           V P L  FFS             P++     L++YIVHV++     +  S N+    W++
Sbjct: 12  VFPLLLCFFS-------------PSSSSSDGLESYIVHVQRSHKPSLFSSHNN----WHV 71

Query: 63  SFLPAMTETVAEPPRLLYAYRNVMSGFSARLTKEQVKSMEKKDGFISAMPETIMNLHTTH 122
           S L ++  +  +P  LLY+Y   + GFSARL+  Q  ++ +    IS +P+    +HTTH
Sbjct: 72  SLLRSLPSS-PQPATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTH 131

Query: 123 TPDYLGLNQQFGLWKNSNFGKGVIIGVLDTGIHPSHPSFSDEGMSPPPAKWKGRCE---- 182
           TP +LG +Q  GLW NSN+G+ VI+GVLDTGI P HPSFSD G+ P P+ WKG CE    
Sbjct: 132 TPAFLGFSQNSGLWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPD 191

Query: 183 FNISVCNNKLIGARNFNRG---------NSFLMGDQSPSDEKGHGTHTASTAAGAFVEGA 242
           F  S CN KLIGAR F RG                +SP D +GHGTHTASTAAG+ V  A
Sbjct: 192 FPASSCNRKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANA 251

Query: 243 KALGNARGKAAGMAPLAHRAIYKVCSVKSCPSSDVLAGIDAAIDDGVDVLSISLG-SRSV 302
                ARG A GMA  A  A YK+C    C  SD+LA +D A+ DGV V+S+S+G S S 
Sbjct: 252 SLYQYARGTATGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSA 311

Query: 303 P-FFKDNIAVATFGAIQKGVFVSCSAGNSGPFNSTLSNEAPWILTVEASTINRRIVAVAK 362
           P +  D+IA+  FGA + G+ VSCSAGNSGP   T +N APWILTV AST++R   A A 
Sbjct: 312 PEYHTDSIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAI 371

Query: 363 LVNEKTKT------LHFVVKVHLKIWMKGGVGR------------VAKGLVVKNAGGAAM 422
             + K  T         +    L +   G  G             V   +V+ + GG A 
Sbjct: 372 TGDGKVFTGTSLYAGESLPDSQLSLVYSGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNAR 431

Query: 423 ILINQ---------------QQDGFSTLSEAHVLPATHVSYEAGLLIKSYINSSQNPTAS 482
           +                    + G    +++H++PAT V  +AG  I+ YI +S +PTA 
Sbjct: 432 VEKGSAVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAK 491

Query: 483 ISFKGTIIGDNDDFSAPAMASFSSRGPCLPSPGILKPDITGPGVNILAAW-----PFPLD 509
           ISF GT+IG +    +P +A+FSSRGP   +P ILKPD+  PGVNILA W     P  LD
Sbjct: 492 ISFLGTLIGPSP--PSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLD 549

BLAST of Lsi09G003410 vs. Swiss-Prot
Match: SBT12_ARATH (Subtilisin-like protease SBT1.2 OS=Arabidopsis thaliana GN=SBT1.2 PE=2 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 6.2e-88
Identity = 221/572 (38.64%), Postives = 304/572 (53.15%), Query Frame = 1

Query: 6   FLCIFFSLNIESNVAAEIELPTTPHQSKLQTYIVHVEQPKIRLVGESSNDHIESWYMSFL 65
           FLCI F L   S+  +EI         + QTYIV +  P        ++     W++SFL
Sbjct: 7   FLCIIFLLFCSSS--SEI--------LQKQTYIVQLH-PNSETAKTFASKF--DWHLSFL 66

Query: 66  P----AMTETVAEPP-RLLYAYRNVMSGFSARLTKEQVKSMEKKDGFISAMPETIMNLHT 125
                 + E   EP  RLLY+Y + + GF+A+LT+ + + +      ++  P+ ++ + T
Sbjct: 67  QEAVLGVEEEEEEPSSRLLYSYGSAIEGFAAQLTESEAEILRYSPEVVAVRPDHVLQVQT 126

Query: 126 THTPDYLGLNQ--QFGLWKNSNFGKGVIIGVLDTGIHPSHPSFSDEGMSPPPAKWKGRCE 185
           T++  +LGL+     G+W  S FG+G IIGVLDTG+ P  PSF D GM   P KWKG C+
Sbjct: 127 TYSYKFLGLDGFGNSGVWSKSRFGQGTIIGVLDTGVWPESPSFDDTGMPSIPRKWKGICQ 186

Query: 186 ----FNISVCNNKLIGARNFNRGNSFLMG-DQSPS---------DEKGHGTHTASTAAGA 245
               F+ S CN KLIGAR F RG+      ++SP+         D  GHGTHTAST  G+
Sbjct: 187 EGESFSSSSCNRKLIGARFFIRGHRVANSPEESPNMPREYISARDSTGHGTHTASTVGGS 246

Query: 246 FVEGAKALGNARGKAAGMAPLAHRAIYKVCSVKSCPSSDVLAGIDAAIDDGVDVLSISLG 305
            V  A  LGN  G A GMAP AH A+YKVC    C SSD+LA ID AI D VDVLS+SLG
Sbjct: 247 SVSMANVLGNGAGVARGMAPGAHIAVYKVCWFNGCYSSDILAAIDVAIQDKVDVLSLSLG 306

Query: 306 SRSVPFFKDNIAVATFGAIQKGVFVSCSAGNSGPFNSTLSN---------------EAPW 365
              +P + D IA+ TF A+++G+ V C+AGN+GP  S+++N                 P 
Sbjct: 307 GFPIPLYDDTIAIGTFRAMERGISVICAAGNNGPIESSVANTAPWVSTIGAGTLDRRFPA 366

Query: 366 ILTVE---------------ASTINRRIVAVAKLVNEKTKTLHF-------VVKVHLKIW 425
           ++ +                     R +  +     +K              ++  + I 
Sbjct: 367 VVRLANGKLLYGESLYPGKGIKNAGREVEVIYVTGGDKGSEFCLRGSLPREEIRGKMVIC 426

Query: 426 MKGGVGRVAKGLVVKNAGGAAMIL----INQQQDGFSTLSEAHVLPATHVSYEAGLLIKS 485
            +G  GR  KG  VK AGG AMIL    INQ++D      + H+LPAT + Y   +L+K+
Sbjct: 427 DRGVNGRSEKGEAVKEAGGVAMILANTEINQEEDSI----DVHLLPATLIGYTESVLLKA 486

Query: 486 YINSSQNPTASISFKGTIIGDNDDFSAPAMASFSSRGPCLPSPGILKPDITGPGVNILAA 509
           Y+N++  P A I F GT+IG +    AP +A FS+RGP L +P ILKPD+  PGVNI+AA
Sbjct: 487 YVNATVKPKARIIFGGTVIGRS---RAPEVAQFSARGPSLANPSILKPDMIAPGVNIIAA 546

BLAST of Lsi09G003410 vs. Swiss-Prot
Match: SBT54_ARATH (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 1.7e-82
Identity = 208/561 (37.08%), Postives = 293/561 (52.23%), Query Frame = 1

Query: 12  SLNIESNVAAEIELPTTPHQSKLQTYIV----HVEQPKIRLVGESSNDHIESWYMSFLPA 71
           SL   S++   + L  +P  +  ++YIV    H   P+I         H    +++    
Sbjct: 17  SLQSLSSLLLLVTLFFSPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVG 76

Query: 72  MTETVAEPPRLLYAYRNVMSGFSARLTKEQVKSMEKKDGFISAMPETIMNLHTTHTPDYL 131
             E   E   + Y+Y+  ++GF+A L + +   + K    +S  P     LHTTH+ +++
Sbjct: 77  SHENAKEA--IFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFM 136

Query: 132 -----GLNQQFGLWKNSNFGKGVIIGVLDTGIHPSHPSFSDEGMSPPPAKWKGRCEFNIS 191
                G+  +  LW  + +G+  II  LDTG+ P   SFSDEG    PA+WKGRC  ++ 
Sbjct: 137 LLAKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCHKDVP 196

Query: 192 VCNNKLIGARNFNRGNSFLMGDQSPS------DEKGHGTHTASTAAGAFVEGAKALGNAR 251
            CN KLIGAR FN+G     G  S +      D  GHG+HT STAAG FV GA   G   
Sbjct: 197 -CNRKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGN 256

Query: 252 GKAAGMAPLAHRAIYKVC----SVKSCPSSDVLAGIDAAIDDGVDVLSISLGSRSVPFFK 311
           G A+G +P A  A YKVC        C  +D+LA I+AAI+DGVDVLS S+G  +  +  
Sbjct: 257 GTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMS 316

Query: 312 DNIAVATFGAIQKGVFVSCSAGNSGPFNSTLSNEAPWILTVEASTINRRIVAVAKLVN-- 371
           D IA+ +F A++ GV V CSAGNSGP + T+SN APW++TV AS+++R   A  +L N  
Sbjct: 317 DGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQ 376

Query: 372 -------------EKTKTLHFVVKVHLK--------IWMKGGV----------------- 431
                        EK  +L      ++         +  KG +                 
Sbjct: 377 SFKGTSLSKPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDN 436

Query: 432 GRVAKGLVVKNAGGAAMILINQQQDGFSTLSEAHVLPATHVSYEAGLLIKSYINSSQNPT 491
            RV KG+    AG A M+L N +  G   +S+AHVLPA+ + Y+ G  + SY++S+++P 
Sbjct: 437 ARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPK 496

Query: 492 ASISFKGTIIGDNDDFSAPAMASFSSRGPCLPSPGILKPDITGPGVNILAAW-----PFP 509
             I      +       AP MASFSSRGP   +PGILKPDIT PGVNI+AA+     P  
Sbjct: 497 GYIKAPTATLNTK---PAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTD 556

BLAST of Lsi09G003410 vs. Swiss-Prot
Match: SBT19_ARATH (Subtilisin-like protease SBT1.9 OS=Arabidopsis thaliana GN=SBT1.9 PE=2 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 4.9e-77
Identity = 198/522 (37.93%), Postives = 284/522 (54.41%), Query Frame = 1

Query: 37  YIVHVEQPKIRLVGESSNDHIESWYMSFLPAMTETVAEPPRLLYAYRNVMSGFSARLTKE 96
           YI+H++     L     +DH  SW+ + L ++       P+++YAY + + GFSA LT  
Sbjct: 25  YIIHMDLSAKPL---PFSDH-RSWFSTTLTSVITN--RKPKIIYAYTDSVHGFSAVLTNS 84

Query: 97  QVKSMEKKDGFISAMPETIMNLHTTHTPDYLGLNQQFGLWKNSNFGKGVIIGVLDTGIHP 156
           +++ ++ K G++S   +  + LHTT +P ++GLN   G W  SN+G G++IG++DTGI P
Sbjct: 85  ELQRLKHKPGYVSFTKDLPVKLHTTFSPKFIGLNSTSGTWPVSNYGAGIVIGIIDTGIWP 144

Query: 157 SHPSFSDEGMSPPPAKWKGRCEFN-ISVCNNKLIGARNFNRG---------NSFLMGDQS 216
             PSF D+G+   P+KWKG CEFN  S+CN KLIGA+ FN+G          + +    S
Sbjct: 145 DSPSFHDDGVGSVPSKWKGACEFNSSSLCNKKLIGAKVFNKGLFANNPDLRETKIGQYSS 204

Query: 217 PSDEKGHGTHTASTAAGAFVEGAKALGNARGKAAGMAPLAHRAIYKVCSVKSCPSSDVLA 276
           P D  GHGTH A+ AAG  V+ A     A+G A+G+AP AH AIYK    +   SSDV+A
Sbjct: 205 PYDTIGHGTHVAAIAAGNHVKNASYFSYAQGTASGIAPHAHLAIYKAAWEEGIYSSDVIA 264

Query: 277 GIDAAIDDGVDVLSISLG--------SRSVPFFKDNIAVATFGAIQKGVFVSCSAGNSGP 336
            ID AI DGV V+S+SLG        +       D IAVA+F AIQKGVFV  S GN GP
Sbjct: 265 AIDQAIRDGVHVISLSLGLSFEDDDDNDGFGLENDPIAVASFAAIQKGVFVVTSGGNDGP 324

Query: 337 FNSTLSNEAPWILTVEASTINRRIVAVAKLVNEKTKTLHFV-------VKVHLKIWMKGG 396
           +  +L N APWI+TV A TI R+        N  + +   +       V+  +     G 
Sbjct: 325 YYWSLINGAPWIMTVGAGTIGRQFQGTLTFGNRVSFSFPSLFPGEFPSVQFPVTYIESGS 384

Query: 397 VGR--VAKGLVV--------------KNAGGAAMILIN----QQQDGFSTLSEAHVLPAT 456
           V    +A  +VV              ++ G AA++LI     ++QD           P  
Sbjct: 385 VENKTLANRIVVCNENINIGSKLHQIRSTGAAAVVLITDKLLEEQDTIK-----FQFPVA 444

Query: 457 HVSYEAGLLIKSYINSSQ-NPTASISFKGTIIGDNDDFSAPAMASFSSRGPCLPSPGILK 508
            +  +    I+SY +S++ N TA + F+ T+IG      AP + ++SSRGP    P ILK
Sbjct: 445 FIGSKHRETIESYASSNKNNATAKLEFRKTVIGTK---PAPEVGTYSSRGPFTSFPQILK 504

BLAST of Lsi09G003410 vs. TrEMBL
Match: A0A0A0KMQ7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G157280 PE=4 SV=1)

HSP 1 Score: 672.9 bits (1735), Expect = 3.0e-190
Identity = 351/517 (67.89%), Postives = 409/517 (79.11%), Query Frame = 1

Query: 32  SKLQTYIVHVEQPKIRLVGESSNDHIESWYMSFLPAMTETVA-EPPRLLYAYRNVMSGFS 91
           + LQTYIVHV+QP++ ++G++ +  +++WY SFLP   E  + E  RLLY+YR+V+SGFS
Sbjct: 11  TNLQTYIVHVKQPEVEILGDTID--LQNWYTSFLPETIEASSNEQSRLLYSYRHVISGFS 70

Query: 92  ARLTKEQVKSMEKKDGFISAMPETIMNLHTTHTPDYLGLNQQFGLWKNSNFGKGVIIGVL 151
           ARLTKEQVK+ME+KDGFISAMPET +NLHTTHTP+YLGLNQ FGLWKNSNFGKGVIIGVL
Sbjct: 71  ARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFGKGVIIGVL 130

Query: 152 DTGIHPSHPSFSDEGMSPPPAKWKGRCEFNISVCNNKLIGARNFNRGNSFLMGDQSPSDE 211
           DTGIHP+HPSF+DEGM  PPAKWKGRCEF  S+CNNKLIGAR FN  N+  +G +SP+DE
Sbjct: 131 DTGIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVSIG-KSPNDE 190

Query: 212 KGHGTHTASTAAGAFVEGAKALGNARGKAAGMAPLAHRAIYKVCSVKSCPSSDVLAGIDA 271
            GHGTHTASTAAG FV+GA+ALGNARGKA GMAPLAH A+YKVCS K C SSD+LA +DA
Sbjct: 191 NGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSPKGCSSSDILAALDA 250

Query: 272 AIDDGVDVLSISLGSRSVPFFKDNIAVATFGAIQKGVFVSCSAGNSGPFNSTLSNEAPWI 331
           AIDDGVDVLS+SLG+ S PFFKD IAV  F AI+KG+FVSCSAGNSGP  +TL+NEAPWI
Sbjct: 251 AIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNTLANEAPWI 310

Query: 332 LTVEASTINRRIVAVAKLVNEKTKT-----------LHFVVKVH---------------- 391
           LTV ASTI+R+IVA+AKL + K  T             F+  V+                
Sbjct: 311 LTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEGSEYCVEGS 370

Query: 392 ---LKIWMK-------GGVGRVAKGLVVKNAGGAAMILINQQQDGFSTLSEAHVLPATHV 451
              L +  K       GG+GR+AKGLVVKN GGAAMIL+NQ+ DGFSTL+EAHVLP TH+
Sbjct: 371 LEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAEAHVLPTTHL 430

Query: 452 SYEAGLLIKSYINSSQNPTASISFKGTIIGDNDDFSAPAMASFSSRGPCLPSPGILKPDI 509
           SYE GL IK YINSS NP ASISF+GT++G+     +PAMASFSSRGPC  SPGILKPDI
Sbjct: 431 SYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQASPGILKPDI 490

BLAST of Lsi09G003410 vs. TrEMBL
Match: A0A0A0KKE3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G157240 PE=4 SV=1)

HSP 1 Score: 629.4 bits (1622), Expect = 3.8e-177
Identity = 344/549 (62.66%), Postives = 401/549 (73.04%), Query Frame = 1

Query: 1   MVVLPFLCIFFSLNIESNVAAEIELPTTPHQSKLQTYIVHVEQPKIRLVGESSNDHIESW 60
           MV+LP L +   LN     A   ELP     S L TYIVHV++P++        D +ESW
Sbjct: 1   MVLLPSLFLLLLLNFHVYEAQVTELPF----SNLHTYIVHVKKPEV-------VDDLESW 60

Query: 61  YMSFLPAMTETVAEPPRLLYAYRNVMSGFSARLTKEQVKSMEKKDGFISAMPETIMNLHT 120
           + SFLP   E   E P LLY+YRNVMSGFSARLT+E VK+ME+KDGF+SA  ETI++LHT
Sbjct: 61  HRSFLPTSLENSEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHT 120

Query: 121 THTPDYLGLNQQFGLWKNSNFGKGVIIGVLDTGIHPSHPSFSDEGMSPPPAKWKGRCEFN 180
           TH+P++LGLN+QFG WK+SNFGKGVIIGVLD GI PSHPSF D GM  PPAKWKGRCEFN
Sbjct: 121 THSPNFLGLNRQFGFWKDSNFGKGVIIGVLDGGITPSHPSFVDAGMPQPPAKWKGRCEFN 180

Query: 181 ISVCNNKLIGARNFNRGNSFLMG-----DQSPSDEKGHGTHTASTAAGAFVEGAKALGNA 240
            S CNNKLIGAR+ N  +  L G     D SP DE GHGTHTASTAAG FV+GA+ALGNA
Sbjct: 181 FSACNNKLIGARSLNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNA 240

Query: 241 RGKAAGMAPLAHRAIYKVCSVKSCPSSDVLAGIDAAIDDGVDVLSISLGSRSVPFFKDNI 300
            G A GMAPLAH AIYKVC  +SC + D+LAG+DAA++DGVDVLSISLG   VPFF D  
Sbjct: 241 FGTAVGMAPLAHLAIYKVCFGESCSNVDILAGLDAAVEDGVDVLSISLGGPPVPFFADIT 300

Query: 301 AVATFGAIQKGVFVSCSAGNSGPFNSTLSNEAPWILTVEASTINRRIVAVAKLVN----- 360
           A+  F AIQKG+FVSCSA NSGPFN+TLSNEAPWILTV ASTI+R+I A AKL N     
Sbjct: 301 AIGAFAAIQKGIFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFD 360

Query: 361 ---------------------EKTKTLHFVVKVHLK-IWMK---------GGVGRVAKGL 420
                                EK +T+    +  LK I +K         GG+ R+AKG+
Sbjct: 361 GESLFQPNDFPQTFLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCDRGGGIARIAKGV 420

Query: 421 VVKNAGGAAMILINQQQDGFSTLSEAHVLPATHVSYEAGLLIKSYINSSQNPTASISFKG 480
            VKNAGGAAMIL+N + DGF+T ++AHVLPA+HVS+ A L IK+YINS+  PTA+I FKG
Sbjct: 421 EVKNAGGAAMILLNAESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPTATIVFKG 480

Query: 481 TIIGDNDDFSAPAMASFSSRGPCLPSPGILKPDITGPGVNILAAWPFPLDNNTNTKSTFN 509
           T IG  DDFS PA+A+FSSRGP L SPGILKPDITGPGV+ILAAWPFPLDNNTNTKSTFN
Sbjct: 481 TTIG--DDFS-PAIAAFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFN 535

BLAST of Lsi09G003410 vs. TrEMBL
Match: B9RBY5_RICCO (Cucumisin, putative OS=Ricinus communis GN=RCOM_1682320 PE=4 SV=1)

HSP 1 Score: 600.9 bits (1548), Expect = 1.4e-168
Identity = 330/553 (59.67%), Postives = 396/553 (71.61%), Query Frame = 1

Query: 1   MVVLPFLCIFFSLNIESNVAAEIELPT-TPHQSKLQTYIVHVEQPKIRLVGESSNDHIES 60
           M V+PF+ + F  N    +A   E  T T  +  LQTYIVHV QP+ R    S  + +++
Sbjct: 10  MTVVPFIFLIFLFNFYPLIAQSAEHTTETIEKKNLQTYIVHVNQPEGRTF--SQPEDLKN 69

Query: 61  WYMSFLPAMTETVAEPP--RLLYAYRNVMSGFSARLTKEQVKSMEKKDGFISAMPETIMN 120
           W+ SFL   T +  E    R+LY+Y+N++SGFSARLT+E+VK+ME+  GF+SA  E  + 
Sbjct: 70  WHKSFLSFSTASSEEEQQQRMLYSYQNIISGFSARLTQEEVKAMEEITGFVSACLERKLR 129

Query: 121 LHTTHTPDYLGLNQQFGLWKNSNFGKGVIIGVLDTGIHPSHPSFSDEGMSPPPAKWKGRC 180
           L TTHTP +LGL+QQ GLWK+S+FGKGVIIG+LD G++PSHPSFSDEGM  PPAKWKGRC
Sbjct: 130 LQTTHTPSFLGLHQQMGLWKDSDFGKGVIIGILDGGVYPSHPSFSDEGMPLPPAKWKGRC 189

Query: 181 EFNISVCNNKLIGARNFNRGNSFLMG--DQSPSDEKGHGTHTASTAAGAFVEGAKALGNA 240
           EFN S CNNKLIGAR FN     + G   + P D  GHGTHTASTAAG FV  +  LGNA
Sbjct: 190 EFNASECNNKLIGARTFNLAAKTMKGAPTEPPIDVDGHGTHTASTAAGGFVYNSDVLGNA 249

Query: 241 RGKAAGMAPLAHRAIYKVC---SVKSCPSSDVLAGIDAAIDDGVDVLSISLGSRSVPFFK 300
           +G A GMAP AH AIYKVC       CP SDVLAG+DAA+DDGVDVLS+SLG  S+PFF+
Sbjct: 250 KGTAVGMAPFAHLAIYKVCFGDPNDDCPESDVLAGLDAAVDDGVDVLSLSLGDVSMPFFQ 309

Query: 301 DNIAVATFGAIQKGVFVSCSAGNSGPFNSTLSNEAPWILTVEASTINRRIVAVAKLVNEK 360
           DNIA+ +F AIQKG+FVSCSAGNSGP  STLSNEAPWILTV ASTI+RRIVA+AKL N +
Sbjct: 310 DNIAIGSFAAIQKGIFVSCSAGNSGPSKSTLSNEAPWILTVGASTIDRRIVAIAKLGNGE 369

Query: 361 --------------TKTLHFV--------------------VKVHLKIWM---KGGVGRV 420
                         T  L  V                    + V  K+ M    GG+GR+
Sbjct: 370 ELDGESVSQPSNFPTTLLPIVYAGMNSKPDSAFCGEGALEGMNVKDKVVMCERGGGIGRI 429

Query: 421 AKGLVVKNAGGAAMILINQQQDGFSTLSEAHVLPATHVSYEAGLLIKSYINSSQNPTASI 480
           AKG  VKNAGGAAMIL+N + +GFST+++AHVLPATHVS+ AGL IK+YINS++ P A+I
Sbjct: 430 AKGDEVKNAGGAAMILVNDETNGFSTIADAHVLPATHVSFAAGLKIKAYINSTKTPMATI 489

Query: 481 SFKGTIIGDNDDFSAPAMASFSSRGPCLPSPGILKPDITGPGVNILAAWPFPLDNNTNTK 509
            FKGT+IGD+   S+PA+ SFSSRGP L SPGILKPDI GPGV+ILAAWPFPLDNNTNTK
Sbjct: 490 LFKGTVIGDS---SSPAVTSFSSRGPSLASPGILKPDIIGPGVSILAAWPFPLDNNTNTK 549

BLAST of Lsi09G003410 vs. TrEMBL
Match: B9GW37_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0003s11870g PE=4 SV=2)

HSP 1 Score: 599.4 bits (1544), Expect = 4.2e-168
Identity = 326/552 (59.06%), Postives = 388/552 (70.29%), Query Frame = 1

Query: 1   MVVLPFLCIFFSLNIESNVAAEIELPTT-PHQSKLQTYIVHVEQPKIRLVGESSNDHIES 60
           M ++P L + F LN    +A   +  T    ++ L  YIVHV +P+ R + E  +  +ES
Sbjct: 1   MAIIPLLLLIFKLNFCPEIAQATQHTTKITEKTTLLNYIVHVAKPEGRTMAEFED--LES 60

Query: 61  WYMSFLPAMTETVAEPPRLLYAYRNVMSGFSARLTKEQVKSMEKKDGFISAMPETIMNLH 120
           WY SFLP  T +  +  R+LYAY+NVMSGF+ARLT+E+VKSME+KDGF+SA PE I++L 
Sbjct: 61  WYQSFLPVSTASSEKQQRMLYAYQNVMSGFAARLTQEEVKSMEEKDGFLSARPERILHLQ 120

Query: 121 TTHTPDYLGLNQQFGLWKNSNFGKGVIIGVLDTGIHPSHPSFSDEGMSPPPAKWKGRCEF 180
           TTHTP +LGL+Q+ G WK SNFGKGVIIGVLD GI PSHPSFSDEGM PPPAKWKGRC+F
Sbjct: 121 TTHTPRFLGLHQELGFWKESNFGKGVIIGVLDGGIFPSHPSFSDEGMPPPPAKWKGRCDF 180

Query: 181 NISVCNNKLIGARNFNRGNSFLMGD---QSPSDEKGHGTHTASTAAGAFVEGAKALGNAR 240
           N S CNNKLIGAR+FN       G    + P D  GHGTHTASTAAGAFV+ A+ LGNAR
Sbjct: 181 NASDCNNKLIGARSFNIAAKAKKGSAATEPPIDVDGHGTHTASTAAGAFVKDAEVLGNAR 240

Query: 241 GKAAGMAPLAHRAIYKVC---SVKSCPSSDVLAGIDAAIDDGVDVLSISLGSRSVPFFKD 300
           G A G+AP AH AIYKVC       CP SD+LAG+DAA+ DGVDVLS+SLG  SVP F D
Sbjct: 241 GTAVGIAPHAHLAIYKVCFGDPGDDCPESDILAGLDAAVQDGVDVLSLSLGEDSVPLFND 300

Query: 301 NIAVATFGAIQKGVFVSCSAGNSGPFNSTLSNEAPWILTVEASTINRRIVAVAKLVN--- 360
            IA+ +F AIQKG+FVSCSAGNSGPFN TLSNEAPWILTV AST++RR  A A+L N   
Sbjct: 301 TIAIGSFAAIQKGIFVSCSAGNSGPFNGTLSNEAPWILTVGASTVDRRFSATARLGNGEQ 360

Query: 361 ---EKTKTLHFVVKVHLKIWMKGGVGR-------------------------------VA 420
              E            L +   G  G+                               +A
Sbjct: 361 IDGESLSQHSNFPSTLLPLVYAGMSGKPNSSLCGEGALEGMDVKGKIVLCERGGGIGRIA 420

Query: 421 KGLVVKNAGGAAMILINQQQDGFSTLSEAHVLPATHVSYEAGLLIKSYINSSQNPTASIS 480
           KG  VKNAGGAAMIL+N++ DGFST ++ HVLPATHVS+ AGL IK+YINS+Q P A+I 
Sbjct: 421 KGGEVKNAGGAAMILMNEEADGFSTNADVHVLPATHVSFAAGLKIKAYINSTQAPMATIL 480

Query: 481 FKGTIIGDNDDFSAPAMASFSSRGPCLPSPGILKPDITGPGVNILAAWPFPLDNNTNTKS 509
           FKGT+IGD+   S+P +ASFSSRGP L SPGILKPDI GPGV+ILAAWPFPLDNNTN+KS
Sbjct: 481 FKGTVIGDS---SSPFVASFSSRGPSLASPGILKPDIIGPGVSILAAWPFPLDNNTNSKS 540

BLAST of Lsi09G003410 vs. TrEMBL
Match: A0A067JNI9_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_20707 PE=4 SV=1)

HSP 1 Score: 594.3 bits (1531), Expect = 1.4e-166
Identity = 321/552 (58.15%), Postives = 392/552 (71.01%), Query Frame = 1

Query: 1   MVVLPFLCIFFSLNIESNVAAEIELPTTPHQSK-LQTYIVHVEQPKIRLVGESSNDHIES 60
           M  +PFL + F  N   N+A   +  T   ++K LQ YIVHV  P+ R   +  N  +E+
Sbjct: 1   MAAIPFLIVIFLFNFYPNIAQAAKHTTEITENKNLQAYIVHVSPPEGRTFSQREN--LEN 60

Query: 61  WYMSFLPAMT-ETVAEPPRLLYAYRNVMSGFSARLTKEQVKSMEKKDGFISAMPETIMNL 120
           W+ SFLP  T  +  +  R+LY+Y N++SGFSARLT E+VK+ME+ +GF+ A PE  ++L
Sbjct: 61  WHKSFLPFSTASSEKQQKRMLYSYHNIISGFSARLTHEEVKAMEEINGFVLARPERKLHL 120

Query: 121 HTTHTPDYLGLNQQFGLWKNSNFGKGVIIGVLDTGIHPSHPSFSDEGMSPPPAKWKGRCE 180
            TTHTP +LGL++Q G WK SNFGKGVIIGVLD G+ PSHPSF+D+GM PPPAKWKGRCE
Sbjct: 121 QTTHTPSFLGLHRQMGFWKESNFGKGVIIGVLDGGVFPSHPSFNDKGMPPPPAKWKGRCE 180

Query: 181 FNISVCNNKLIGARNFNRGNSFLMG--DQSPSDEKGHGTHTASTAAGAFVEGAKALGNAR 240
           FN S CNNKLIGAR+FN     + G   ++P D  GHGTHTASTAAG+FV  A  LGNA+
Sbjct: 181 FNASKCNNKLIGARSFNLAAKAMKGIAAETPIDVDGHGTHTASTAAGSFVYNANVLGNAK 240

Query: 241 GKAAGMAPLAHRAIYKVC---SVKSCPSSDVLAGIDAAIDDGVDVLSISLGSRSVPFFKD 300
           G A GMAP AH AIYKVC       CP SD+LAG+DAAI DGVDVLS+S+G  S+PFF+D
Sbjct: 241 GTAVGMAPYAHLAIYKVCFGDPNDDCPESDILAGLDAAIQDGVDVLSLSIGDISMPFFQD 300

Query: 301 NIAVATFGAIQKGVFVSCSAGNSGPFNSTLSNEAPWILTVEASTINRRIVAVAKL----- 360
           NIA+ +F AIQKG+FVSC+AGNSGPFN TLSNEAPWILTV ASTI+R+I A AKL     
Sbjct: 301 NIAIGSFAAIQKGIFVSCAAGNSGPFNGTLSNEAPWILTVGASTIDRKIAATAKLGNGEE 360

Query: 361 -----------------------VNEKTKTLHFVVKVHLKIWMK---------GGVGRVA 420
                                  +N KT++     +    + +K         GG+GRVA
Sbjct: 361 LDGESVLQPSNFPTTLLPLVYPGMNGKTESAFCSERAVQGMDVKDKVVLCERGGGIGRVA 420

Query: 421 KGLVVKNAGGAAMILINQQQDGFSTLSEAHVLPATHVSYEAGLLIKSYINSSQNPTASIS 480
           KG  VKNAGGAAMILIN +  GFST+++AHVLPATHVS+ AGL IK+YINS++ P A+I 
Sbjct: 421 KGEEVKNAGGAAMILINDEISGFSTIADAHVLPATHVSFAAGLQIKAYINSTKTPMATIL 480

Query: 481 FKGTIIGDNDDFSAPAMASFSSRGPCLPSPGILKPDITGPGVNILAAWPFPLDNNTNTKS 509
           FKGT+IGD     +PA+ SFSSRGP L SPGILKPDI GPGV+ILAAWPFPLDN TNTKS
Sbjct: 481 FKGTVIGDP---LSPAVTSFSSRGPNLASPGILKPDIIGPGVSILAAWPFPLDNTTNTKS 540

BLAST of Lsi09G003410 vs. TAIR10
Match: AT5G67360.1 (AT5G67360.1 Subtilase family protein)

HSP 1 Score: 380.9 bits (977), Expect = 1.2e-105
Identity = 237/527 (44.97%), Postives = 312/527 (59.20%), Query Frame = 1

Query: 36  TYIVHVEQPKIRLVGESSNDHIESWYMSFLPAMTETVAEPPRLLYAYRNVMSGFSARLTK 95
           TYIVH+ + ++     SS D   +WY S L     ++++   LLY Y N + GFS RLT+
Sbjct: 31  TYIVHMAKSQM----PSSFDLHSNWYDSSL----RSISDSAELLYTYENAIHGFSTRLTQ 90

Query: 96  EQVKSMEKKDGFISAMPETIMNLHTTHTPDYLGLNQQFG-LWKNSNFGKGVIIGVLDTGI 155
           E+  S+  + G IS +PE    LHTT TP +LGL++    L+  +     V++GVLDTG+
Sbjct: 91  EEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYSDVVVGVLDTGV 150

Query: 156 HPSHPSFSDEGMSPPPAKWKGRCE----FNISVCNNKLIGARNFNRGNSFLMG------- 215
            P   S+SDEG  P P+ WKG CE    F  S+CN KLIGAR F RG    MG       
Sbjct: 151 WPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGPIDESKE 210

Query: 216 DQSPSDEKGHGTHTASTAAGAFVEGAKALGNARGKAAGMAPLAHRAIYKVCSVKSCPSSD 275
            +SP D+ GHGTHT+STAAG+ VEGA  LG A G A GMAP A  A+YKVC +  C SSD
Sbjct: 211 SRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWLGGCFSSD 270

Query: 276 VLAGIDAAIDDGVDVLSISLGSRSVPFFKDNIAVATFGAIQKGVFVSCSAGNSGPFNSTL 335
           +LA ID AI D V+VLS+SLG     +++D +A+  F A+++G+ VSCSAGN+GP +S+L
Sbjct: 271 ILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSL 330

Query: 336 SNEAPWILTVEASTINRRIVAVAKLVNEKT--------------KTLHFVV--------- 395
           SN APWI TV A T++R   A+A L N K               K L F+          
Sbjct: 331 SNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFIYAGNASNATN 390

Query: 396 ------------KVHLKIWM--KGGVGRVAKGLVVKNAGGAAMILINQQQDGFSTLSEAH 455
                       KV  KI M  +G   RV KG VVK AGG  MIL N   +G   +++AH
Sbjct: 391 GNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAH 450

Query: 456 VLPATHVSYEAGLLIKSYINSSQNPTASISFKGTIIGDNDDFSAPAMASFSSRGPCLPSP 509
           +LPAT V  +AG +I+ Y+ +  NPTASIS  GT++G      +P +A+FSSRGP   +P
Sbjct: 451 LLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVK---PSPVVAAFSSRGPNSITP 510

BLAST of Lsi09G003410 vs. TAIR10
Match: AT3G14067.1 (AT3G14067.1 Subtilase family protein)

HSP 1 Score: 352.4 bits (903), Expect = 4.5e-97
Identity = 230/559 (41.14%), Postives = 306/559 (54.74%), Query Frame = 1

Query: 3   VLPFLCIFFSLNIESNVAAEIELPTTPHQSKLQTYIVHVEQPKIRLVGESSNDHIESWYM 62
           V P L  FFS             P++     L++YIVHV++     +  S N+    W++
Sbjct: 12  VFPLLLCFFS-------------PSSSSSDGLESYIVHVQRSHKPSLFSSHNN----WHV 71

Query: 63  SFLPAMTETVAEPPRLLYAYRNVMSGFSARLTKEQVKSMEKKDGFISAMPETIMNLHTTH 122
           S L ++  +  +P  LLY+Y   + GFSARL+  Q  ++ +    IS +P+    +HTTH
Sbjct: 72  SLLRSLPSS-PQPATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTH 131

Query: 123 TPDYLGLNQQFGLWKNSNFGKGVIIGVLDTGIHPSHPSFSDEGMSPPPAKWKGRCE---- 182
           TP +LG +Q  GLW NSN+G+ VI+GVLDTGI P HPSFSD G+ P P+ WKG CE    
Sbjct: 132 TPAFLGFSQNSGLWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPD 191

Query: 183 FNISVCNNKLIGARNFNRG---------NSFLMGDQSPSDEKGHGTHTASTAAGAFVEGA 242
           F  S CN KLIGAR F RG                +SP D +GHGTHTASTAAG+ V  A
Sbjct: 192 FPASSCNRKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANA 251

Query: 243 KALGNARGKAAGMAPLAHRAIYKVCSVKSCPSSDVLAGIDAAIDDGVDVLSISLG-SRSV 302
                ARG A GMA  A  A YK+C    C  SD+LA +D A+ DGV V+S+S+G S S 
Sbjct: 252 SLYQYARGTATGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSA 311

Query: 303 P-FFKDNIAVATFGAIQKGVFVSCSAGNSGPFNSTLSNEAPWILTVEASTINRRIVAVAK 362
           P +  D+IA+  FGA + G+ VSCSAGNSGP   T +N APWILTV AST++R   A A 
Sbjct: 312 PEYHTDSIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAI 371

Query: 363 LVNEKTKT------LHFVVKVHLKIWMKGGVGR------------VAKGLVVKNAGGAAM 422
             + K  T         +    L +   G  G             V   +V+ + GG A 
Sbjct: 372 TGDGKVFTGTSLYAGESLPDSQLSLVYSGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNAR 431

Query: 423 ILINQ---------------QQDGFSTLSEAHVLPATHVSYEAGLLIKSYINSSQNPTAS 482
           +                    + G    +++H++PAT V  +AG  I+ YI +S +PTA 
Sbjct: 432 VEKGSAVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAK 491

Query: 483 ISFKGTIIGDNDDFSAPAMASFSSRGPCLPSPGILKPDITGPGVNILAAW-----PFPLD 509
           ISF GT+IG +    +P +A+FSSRGP   +P ILKPD+  PGVNILA W     P  LD
Sbjct: 492 ISFLGTLIGPSP--PSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLD 549

BLAST of Lsi09G003410 vs. TAIR10
Match: AT1G04110.1 (AT1G04110.1 Subtilase family protein)

HSP 1 Score: 326.2 bits (835), Expect = 3.5e-89
Identity = 221/572 (38.64%), Postives = 304/572 (53.15%), Query Frame = 1

Query: 6   FLCIFFSLNIESNVAAEIELPTTPHQSKLQTYIVHVEQPKIRLVGESSNDHIESWYMSFL 65
           FLCI F L   S+  +EI         + QTYIV +  P        ++     W++SFL
Sbjct: 7   FLCIIFLLFCSSS--SEI--------LQKQTYIVQLH-PNSETAKTFASKF--DWHLSFL 66

Query: 66  P----AMTETVAEPP-RLLYAYRNVMSGFSARLTKEQVKSMEKKDGFISAMPETIMNLHT 125
                 + E   EP  RLLY+Y + + GF+A+LT+ + + +      ++  P+ ++ + T
Sbjct: 67  QEAVLGVEEEEEEPSSRLLYSYGSAIEGFAAQLTESEAEILRYSPEVVAVRPDHVLQVQT 126

Query: 126 THTPDYLGLNQ--QFGLWKNSNFGKGVIIGVLDTGIHPSHPSFSDEGMSPPPAKWKGRCE 185
           T++  +LGL+     G+W  S FG+G IIGVLDTG+ P  PSF D GM   P KWKG C+
Sbjct: 127 TYSYKFLGLDGFGNSGVWSKSRFGQGTIIGVLDTGVWPESPSFDDTGMPSIPRKWKGICQ 186

Query: 186 ----FNISVCNNKLIGARNFNRGNSFLMG-DQSPS---------DEKGHGTHTASTAAGA 245
               F+ S CN KLIGAR F RG+      ++SP+         D  GHGTHTAST  G+
Sbjct: 187 EGESFSSSSCNRKLIGARFFIRGHRVANSPEESPNMPREYISARDSTGHGTHTASTVGGS 246

Query: 246 FVEGAKALGNARGKAAGMAPLAHRAIYKVCSVKSCPSSDVLAGIDAAIDDGVDVLSISLG 305
            V  A  LGN  G A GMAP AH A+YKVC    C SSD+LA ID AI D VDVLS+SLG
Sbjct: 247 SVSMANVLGNGAGVARGMAPGAHIAVYKVCWFNGCYSSDILAAIDVAIQDKVDVLSLSLG 306

Query: 306 SRSVPFFKDNIAVATFGAIQKGVFVSCSAGNSGPFNSTLSN---------------EAPW 365
              +P + D IA+ TF A+++G+ V C+AGN+GP  S+++N                 P 
Sbjct: 307 GFPIPLYDDTIAIGTFRAMERGISVICAAGNNGPIESSVANTAPWVSTIGAGTLDRRFPA 366

Query: 366 ILTVE---------------ASTINRRIVAVAKLVNEKTKTLHF-------VVKVHLKIW 425
           ++ +                     R +  +     +K              ++  + I 
Sbjct: 367 VVRLANGKLLYGESLYPGKGIKNAGREVEVIYVTGGDKGSEFCLRGSLPREEIRGKMVIC 426

Query: 426 MKGGVGRVAKGLVVKNAGGAAMIL----INQQQDGFSTLSEAHVLPATHVSYEAGLLIKS 485
            +G  GR  KG  VK AGG AMIL    INQ++D      + H+LPAT + Y   +L+K+
Sbjct: 427 DRGVNGRSEKGEAVKEAGGVAMILANTEINQEEDSI----DVHLLPATLIGYTESVLLKA 486

Query: 486 YINSSQNPTASISFKGTIIGDNDDFSAPAMASFSSRGPCLPSPGILKPDITGPGVNILAA 509
           Y+N++  P A I F GT+IG +    AP +A FS+RGP L +P ILKPD+  PGVNI+AA
Sbjct: 487 YVNATVKPKARIIFGGTVIGRS---RAPEVAQFSARGPSLANPSILKPDMIAPGVNIIAA 546

BLAST of Lsi09G003410 vs. TAIR10
Match: AT5G59810.1 (AT5G59810.1 Subtilase family protein)

HSP 1 Score: 308.1 bits (788), Expect = 9.8e-84
Identity = 208/561 (37.08%), Postives = 293/561 (52.23%), Query Frame = 1

Query: 12  SLNIESNVAAEIELPTTPHQSKLQTYIV----HVEQPKIRLVGESSNDHIESWYMSFLPA 71
           SL   S++   + L  +P  +  ++YIV    H   P+I         H    +++    
Sbjct: 17  SLQSLSSLLLLVTLFFSPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVG 76

Query: 72  MTETVAEPPRLLYAYRNVMSGFSARLTKEQVKSMEKKDGFISAMPETIMNLHTTHTPDYL 131
             E   E   + Y+Y+  ++GF+A L + +   + K    +S  P     LHTTH+ +++
Sbjct: 77  SHENAKEA--IFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFM 136

Query: 132 -----GLNQQFGLWKNSNFGKGVIIGVLDTGIHPSHPSFSDEGMSPPPAKWKGRCEFNIS 191
                G+  +  LW  + +G+  II  LDTG+ P   SFSDEG    PA+WKGRC  ++ 
Sbjct: 137 LLAKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCHKDVP 196

Query: 192 VCNNKLIGARNFNRGNSFLMGDQSPS------DEKGHGTHTASTAAGAFVEGAKALGNAR 251
            CN KLIGAR FN+G     G  S +      D  GHG+HT STAAG FV GA   G   
Sbjct: 197 -CNRKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGN 256

Query: 252 GKAAGMAPLAHRAIYKVC----SVKSCPSSDVLAGIDAAIDDGVDVLSISLGSRSVPFFK 311
           G A+G +P A  A YKVC        C  +D+LA I+AAI+DGVDVLS S+G  +  +  
Sbjct: 257 GTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMS 316

Query: 312 DNIAVATFGAIQKGVFVSCSAGNSGPFNSTLSNEAPWILTVEASTINRRIVAVAKLVN-- 371
           D IA+ +F A++ GV V CSAGNSGP + T+SN APW++TV AS+++R   A  +L N  
Sbjct: 317 DGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQ 376

Query: 372 -------------EKTKTLHFVVKVHLK--------IWMKGGV----------------- 431
                        EK  +L      ++         +  KG +                 
Sbjct: 377 SFKGTSLSKPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDN 436

Query: 432 GRVAKGLVVKNAGGAAMILINQQQDGFSTLSEAHVLPATHVSYEAGLLIKSYINSSQNPT 491
            RV KG+    AG A M+L N +  G   +S+AHVLPA+ + Y+ G  + SY++S+++P 
Sbjct: 437 ARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPK 496

Query: 492 ASISFKGTIIGDNDDFSAPAMASFSSRGPCLPSPGILKPDITGPGVNILAAW-----PFP 509
             I      +       AP MASFSSRGP   +PGILKPDIT PGVNI+AA+     P  
Sbjct: 497 GYIKAPTATLNTK---PAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTD 556

BLAST of Lsi09G003410 vs. TAIR10
Match: AT5G67090.1 (AT5G67090.1 Subtilisin-like serine endopeptidase family protein)

HSP 1 Score: 290.0 bits (741), Expect = 2.8e-78
Identity = 198/522 (37.93%), Postives = 284/522 (54.41%), Query Frame = 1

Query: 37  YIVHVEQPKIRLVGESSNDHIESWYMSFLPAMTETVAEPPRLLYAYRNVMSGFSARLTKE 96
           YI+H++     L     +DH  SW+ + L ++       P+++YAY + + GFSA LT  
Sbjct: 25  YIIHMDLSAKPL---PFSDH-RSWFSTTLTSVITN--RKPKIIYAYTDSVHGFSAVLTNS 84

Query: 97  QVKSMEKKDGFISAMPETIMNLHTTHTPDYLGLNQQFGLWKNSNFGKGVIIGVLDTGIHP 156
           +++ ++ K G++S   +  + LHTT +P ++GLN   G W  SN+G G++IG++DTGI P
Sbjct: 85  ELQRLKHKPGYVSFTKDLPVKLHTTFSPKFIGLNSTSGTWPVSNYGAGIVIGIIDTGIWP 144

Query: 157 SHPSFSDEGMSPPPAKWKGRCEFN-ISVCNNKLIGARNFNRG---------NSFLMGDQS 216
             PSF D+G+   P+KWKG CEFN  S+CN KLIGA+ FN+G          + +    S
Sbjct: 145 DSPSFHDDGVGSVPSKWKGACEFNSSSLCNKKLIGAKVFNKGLFANNPDLRETKIGQYSS 204

Query: 217 PSDEKGHGTHTASTAAGAFVEGAKALGNARGKAAGMAPLAHRAIYKVCSVKSCPSSDVLA 276
           P D  GHGTH A+ AAG  V+ A     A+G A+G+AP AH AIYK    +   SSDV+A
Sbjct: 205 PYDTIGHGTHVAAIAAGNHVKNASYFSYAQGTASGIAPHAHLAIYKAAWEEGIYSSDVIA 264

Query: 277 GIDAAIDDGVDVLSISLG--------SRSVPFFKDNIAVATFGAIQKGVFVSCSAGNSGP 336
            ID AI DGV V+S+SLG        +       D IAVA+F AIQKGVFV  S GN GP
Sbjct: 265 AIDQAIRDGVHVISLSLGLSFEDDDDNDGFGLENDPIAVASFAAIQKGVFVVTSGGNDGP 324

Query: 337 FNSTLSNEAPWILTVEASTINRRIVAVAKLVNEKTKTLHFV-------VKVHLKIWMKGG 396
           +  +L N APWI+TV A TI R+        N  + +   +       V+  +     G 
Sbjct: 325 YYWSLINGAPWIMTVGAGTIGRQFQGTLTFGNRVSFSFPSLFPGEFPSVQFPVTYIESGS 384

Query: 397 VGR--VAKGLVV--------------KNAGGAAMILIN----QQQDGFSTLSEAHVLPAT 456
           V    +A  +VV              ++ G AA++LI     ++QD           P  
Sbjct: 385 VENKTLANRIVVCNENINIGSKLHQIRSTGAAAVVLITDKLLEEQDTIK-----FQFPVA 444

Query: 457 HVSYEAGLLIKSYINSSQ-NPTASISFKGTIIGDNDDFSAPAMASFSSRGPCLPSPGILK 508
            +  +    I+SY +S++ N TA + F+ T+IG      AP + ++SSRGP    P ILK
Sbjct: 445 FIGSKHRETIESYASSNKNNATAKLEFRKTVIGTK---PAPEVGTYSSRGPFTSFPQILK 504

BLAST of Lsi09G003410 vs. NCBI nr
Match: gi|659074655|ref|XP_008437722.1| (PREDICTED: subtilisin-like protease [Cucumis melo])

HSP 1 Score: 836.6 bits (2160), Expect = 2.3e-239
Identity = 433/540 (80.19%), Postives = 469/540 (86.85%), Query Frame = 1

Query: 6   FLCIFFSLNIESNVAAEIELPTTPHQSKLQTYIVHVEQPKIRLVGESSNDHIESWYMSFL 65
           F   FF LNIES+VAA++ELPTTP QSKLQTYIVHVEQPKIRLVGESSNDHIESWYMSF+
Sbjct: 15  FPLYFFLLNIESHVAAKVELPTTPLQSKLQTYIVHVEQPKIRLVGESSNDHIESWYMSFI 74

Query: 66  PAMTETVAEPPRLLYAYRNVMSGFSARLTKEQVKSMEKKDGFISAMPETIMNLHTTHTPD 125
           P  TET  E P+LLY+YRNVMSGFSARLT EQVK+MEKKDGFISAMPETIM+LHTTHTP+
Sbjct: 75  PKSTETTVEQPQLLYSYRNVMSGFSARLTIEQVKAMEKKDGFISAMPETIMSLHTTHTPE 134

Query: 126 YLGLNQQFGLWKNSNFGKGVIIGVLDTGIHPSHPSFSDEGMSPPPAKWKGRCEFNISVCN 185
           YLGLNQQFG+WKNSNFGKGVI+GVLDTGIHP+HPSF+DEGMSPPPAKWKG+CEFN S+CN
Sbjct: 135 YLGLNQQFGIWKNSNFGKGVIVGVLDTGIHPNHPSFNDEGMSPPPAKWKGKCEFNSSLCN 194

Query: 186 NKLIGARNFNRGNSFLMGDQSPSDEKGHGTHTASTAAGAFVEGAKALGNARGKAAGMAPL 245
           NKLIGAR FN GN+FLM ++SP+DEKGHGTHTASTAAGAFVE A+ALGNA+GKAAG+APL
Sbjct: 195 NKLIGARTFNLGNNFLM-EESPNDEKGHGTHTASTAAGAFVEDAEALGNAKGKAAGIAPL 254

Query: 246 AHRAIYKVCSVKSCPSSDVLAGIDAAIDDGVDVLSISLGSRSVPFFKDNIAVATFGAIQK 305
           AH AIYKVCS K CPSSDV AGIDAAIDDGVDVLSISLGSRSVPFFKDNIAVATFGAIQK
Sbjct: 255 AHLAIYKVCSGKRCPSSDVFAGIDAAIDDGVDVLSISLGSRSVPFFKDNIAVATFGAIQK 314

Query: 306 GVFVSCSAGNSGPFNSTLSNEAPWILTVEASTINRRIVAVAKL----------------- 365
           G+FVS SAGNSGP NSTLSN+APWILTV ASTINRRIVAVAKL                 
Sbjct: 315 GIFVSSSAGNSGPLNSTLSNDAPWILTVGASTINRRIVAVAKLGNGEQYEGESLYQPNDF 374

Query: 366 ----------VNEKTKTLHFV-------VKVHLKIWM---KGGVGRVAKGLVVKNAGGAA 425
                      N + KT  F        + V  K+ +   KGGVGRVAKGLVVKNAGGAA
Sbjct: 375 PSKFLPLVYAGNRENKTYAFCGEGSLENMDVKGKVVVCEGKGGVGRVAKGLVVKNAGGAA 434

Query: 426 MILINQQQDGFSTLSEAHVLPATHVSYEAGLLIKSYINSSQNPTASISFKGTIIGDNDDF 485
           MILINQ++DGFSTLSEAHVLPATHVSY+AG+LIKSYINSSQNPTASISFKGT+IGD DDF
Sbjct: 435 MILINQEEDGFSTLSEAHVLPATHVSYKAGVLIKSYINSSQNPTASISFKGTVIGDGDDF 494

Query: 486 SAPAMASFSSRGPCLPSPGILKPDITGPGVNILAAWPFPLDNNTNTKSTFNVMSGTSMSC 509
           SAP+MASFSSRGPCLPSPGILKPDITGPGVNILAAWPFPLDN+TNTKSTFNV+SGTSMSC
Sbjct: 495 SAPSMASFSSRGPCLPSPGILKPDITGPGVNILAAWPFPLDNDTNTKSTFNVISGTSMSC 553

BLAST of Lsi09G003410 vs. NCBI nr
Match: gi|659073650|ref|XP_008437177.1| (PREDICTED: subtilisin-like protease [Cucumis melo])

HSP 1 Score: 696.4 bits (1796), Expect = 3.6e-197
Identity = 367/548 (66.97%), Postives = 431/548 (78.65%), Query Frame = 1

Query: 1   MVVLPFLCIFFSLNIESNVAAEIELPTTPHQSKLQTYIVHVEQPKIRLVGESSNDHIESW 60
           MV LPF+CIFF LN    VA + EL +T + + LQTYIVHV+QP + ++G++ +  +++W
Sbjct: 1   MVFLPFICIFFFLNFHGYVAMKTELSSTSN-TNLQTYIVHVKQPVLEILGDTID--LQNW 60

Query: 61  YMSFLPAMTETVA-EPPRLLYAYRNVMSGFSARLTKEQVKSMEKKDGFISAMPETIMNLH 120
           Y SFLP   E  + E PRLLY+YR+VMSGFSARLTKEQVK+ME+KDGFISAMPET +NLH
Sbjct: 61  YTSFLPETIEASSDEQPRLLYSYRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLH 120

Query: 121 TTHTPDYLGLNQQFGLWKNSNFGKGVIIGVLDTGIHPSHPSFSDEGMSPPPAKWKGRCEF 180
           TTHTP+YLGLN+ FGLWKNSNFGKGVIIGVLDTGIHP+HPSF+DEGMS PPAKWKGRCEF
Sbjct: 121 TTHTPEYLGLNKHFGLWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEF 180

Query: 181 NISVCNNKLIGARNFNRGNSFLMGDQSPSDEKGHGTHTASTAAGAFVEGAKALGNARGKA 240
             S+CNNKLIGAR FN  N+F++G +SP+DE GHGTHTASTAAG FV+GA+ALGNARGKA
Sbjct: 181 GASICNNKLIGARTFNLANNFVIG-KSPNDENGHGTHTASTAAGTFVKGAEALGNARGKA 240

Query: 241 AGMAPLAHRAIYKVCSVKSCPSSDVLAGIDAAIDDGVDVLSISLGSRSVPFFKDNIAVAT 300
            GMAPLAH A+YKVCS   C SSD+LA +DAAIDDGVDVLS+SLG+ S PFF+D IA+  
Sbjct: 241 VGMAPLAHIAMYKVCSPNGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAMGA 300

Query: 301 FGAIQKGVFVSCSAGNSGPFNSTLSNEAPWILTVEASTINRRIVAVAKLVNEKTKT---- 360
           F AI+KG+FVSCSAGNSGP  +TL+NEAPWILTV ASTI+R+IVA+AKL + +  T    
Sbjct: 301 FAAIKKGIFVSCSAGNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESL 360

Query: 361 -------LHFVVKVH-------------------LKIWMK-------GGVGRVAKGLVVK 420
                    F+  V+                   L +  K       GG+ RVAKGLVVK
Sbjct: 361 FQPRNFPSKFLPLVYAGKSGIEGSEYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVK 420

Query: 421 NAGGAAMILINQQQDGFSTLSEAHVLPATHVSYEAGLLIKSYINSSQNPTASISFKGTII 480
           N GGAAMILINQ+ +GFSTL+EAHVLP TH+SYE GL IK+YINSS NP ASISFKGT++
Sbjct: 421 NGGGAAMILINQKPEGFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLL 480

Query: 481 GDNDDFSAPAMASFSSRGPCLPSPGILKPDITGPGVNILAAWPFPLDN--NTNTKSTFNV 509
           G+     +PAMASFSSRGPC  SPGILKPDITGPGVNILAAWPFPL+N  NTNTKSTFNV
Sbjct: 481 GNRATTFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNV 540

BLAST of Lsi09G003410 vs. NCBI nr
Match: gi|449459730|ref|XP_004147599.1| (PREDICTED: subtilisin-like protease SBT1.7 [Cucumis sativus])

HSP 1 Score: 672.9 bits (1735), Expect = 4.3e-190
Identity = 351/517 (67.89%), Postives = 409/517 (79.11%), Query Frame = 1

Query: 32  SKLQTYIVHVEQPKIRLVGESSNDHIESWYMSFLPAMTETVA-EPPRLLYAYRNVMSGFS 91
           + LQTYIVHV+QP++ ++G++ +  +++WY SFLP   E  + E  RLLY+YR+V+SGFS
Sbjct: 11  TNLQTYIVHVKQPEVEILGDTID--LQNWYTSFLPETIEASSNEQSRLLYSYRHVISGFS 70

Query: 92  ARLTKEQVKSMEKKDGFISAMPETIMNLHTTHTPDYLGLNQQFGLWKNSNFGKGVIIGVL 151
           ARLTKEQVK+ME+KDGFISAMPET +NLHTTHTP+YLGLNQ FGLWKNSNFGKGVIIGVL
Sbjct: 71  ARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFGKGVIIGVL 130

Query: 152 DTGIHPSHPSFSDEGMSPPPAKWKGRCEFNISVCNNKLIGARNFNRGNSFLMGDQSPSDE 211
           DTGIHP+HPSF+DEGM  PPAKWKGRCEF  S+CNNKLIGAR FN  N+  +G +SP+DE
Sbjct: 131 DTGIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVSIG-KSPNDE 190

Query: 212 KGHGTHTASTAAGAFVEGAKALGNARGKAAGMAPLAHRAIYKVCSVKSCPSSDVLAGIDA 271
            GHGTHTASTAAG FV+GA+ALGNARGKA GMAPLAH A+YKVCS K C SSD+LA +DA
Sbjct: 191 NGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSPKGCSSSDILAALDA 250

Query: 272 AIDDGVDVLSISLGSRSVPFFKDNIAVATFGAIQKGVFVSCSAGNSGPFNSTLSNEAPWI 331
           AIDDGVDVLS+SLG+ S PFFKD IAV  F AI+KG+FVSCSAGNSGP  +TL+NEAPWI
Sbjct: 251 AIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNTLANEAPWI 310

Query: 332 LTVEASTINRRIVAVAKLVNEKTKT-----------LHFVVKVH---------------- 391
           LTV ASTI+R+IVA+AKL + K  T             F+  V+                
Sbjct: 311 LTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEGSEYCVEGS 370

Query: 392 ---LKIWMK-------GGVGRVAKGLVVKNAGGAAMILINQQQDGFSTLSEAHVLPATHV 451
              L +  K       GG+GR+AKGLVVKN GGAAMIL+NQ+ DGFSTL+EAHVLP TH+
Sbjct: 371 LEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAEAHVLPTTHL 430

Query: 452 SYEAGLLIKSYINSSQNPTASISFKGTIIGDNDDFSAPAMASFSSRGPCLPSPGILKPDI 509
           SYE GL IK YINSS NP ASISF+GT++G+     +PAMASFSSRGPC  SPGILKPDI
Sbjct: 431 SYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQASPGILKPDI 490

BLAST of Lsi09G003410 vs. NCBI nr
Match: gi|659073656|ref|XP_008437181.1| (PREDICTED: subtilisin-like protease [Cucumis melo])

HSP 1 Score: 629.8 bits (1623), Expect = 4.2e-177
Identity = 343/549 (62.48%), Postives = 399/549 (72.68%), Query Frame = 1

Query: 1   MVVLPFLCIFFSLNIESNVAAEIELPTTPHQSKLQTYIVHVEQPKIRLVGESSNDHIESW 60
           MV+LP L +   LN     A   ELP     S L TYIVHV++P++        D +E W
Sbjct: 1   MVLLPSLFLLLLLNFHGYEAQVTELPL----SNLHTYIVHVKKPEV-------VDDLEIW 60

Query: 61  YMSFLPAMTETVAEPPRLLYAYRNVMSGFSARLTKEQVKSMEKKDGFISAMPETIMNLHT 120
           + SFLP   +   E P LLY+YRNVMSGFSARLT+E VK+ME+KDGF+SA  ETI++LHT
Sbjct: 61  HRSFLPTSLDNEEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHT 120

Query: 121 THTPDYLGLNQQFGLWKNSNFGKGVIIGVLDTGIHPSHPSFSDEGMSPPPAKWKGRCEFN 180
           THTPD+LGLN+QFG WK+SNFGKGVIIGVLD GI P+HPSF D GM+ PPAKWKGRCEFN
Sbjct: 121 THTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMAQPPAKWKGRCEFN 180

Query: 181 ISVCNNKLIGARNFNRGNSFLMG-----DQSPSDEKGHGTHTASTAAGAFVEGAKALGNA 240
            S CNNKLIGAR+ N  +  L G     D SP DE GHGTHTASTAAG FV+GA+ALGNA
Sbjct: 181 FSACNNKLIGARSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNA 240

Query: 241 RGKAAGMAPLAHRAIYKVCSVKSCPSSDVLAGIDAAIDDGVDVLSISLGSRSVPFFKDNI 300
            G A GMAPLAH AIYKVC  + C   D+LAG+DAA++DGVDVLSISLG  SVPFF D  
Sbjct: 241 FGTAVGMAPLAHLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADIT 300

Query: 301 AVATFGAIQKGVFVSCSAGNSGPFNSTLSNEAPWILTVEASTINRRIVAVAKLVN----- 360
           A+ +F AIQKG+FVSCSA NSGPFN+TLSNEAPWILTV ASTI+R+I A AKL N     
Sbjct: 301 AIGSFAAIQKGIFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFD 360

Query: 361 ---------------------EKTKTLHFVVKVHLK-IWMK---------GGVGRVAKGL 420
                                EK +T+    +  LK I +K         GG+ R+AKG+
Sbjct: 361 GESLFQPNDFPQTLLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGV 420

Query: 421 VVKNAGGAAMILINQQQDGFSTLSEAHVLPATHVSYEAGLLIKSYINSSQNPTASISFKG 480
            VKN GGAAMIL+N + DGF+T  +AHVLPA+HVS+ A L IK+YINS+  PTA+I FKG
Sbjct: 421 EVKNGGGAAMILLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKG 480

Query: 481 TIIGDNDDFSAPAMASFSSRGPCLPSPGILKPDITGPGVNILAAWPFPLDNNTNTKSTFN 509
           T IG  DDFS PA+ASFSSRGP L SPGILKPDITGPGV+ILAAWPFPLDNNTNTKSTFN
Sbjct: 481 TTIG--DDFS-PAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFN 535

BLAST of Lsi09G003410 vs. NCBI nr
Match: gi|449459724|ref|XP_004147596.1| (PREDICTED: subtilisin-like protease SBT1.2 [Cucumis sativus])

HSP 1 Score: 629.4 bits (1622), Expect = 5.5e-177
Identity = 344/549 (62.66%), Postives = 401/549 (73.04%), Query Frame = 1

Query: 1   MVVLPFLCIFFSLNIESNVAAEIELPTTPHQSKLQTYIVHVEQPKIRLVGESSNDHIESW 60
           MV+LP L +   LN     A   ELP     S L TYIVHV++P++        D +ESW
Sbjct: 1   MVLLPSLFLLLLLNFHVYEAQVTELPF----SNLHTYIVHVKKPEV-------VDDLESW 60

Query: 61  YMSFLPAMTETVAEPPRLLYAYRNVMSGFSARLTKEQVKSMEKKDGFISAMPETIMNLHT 120
           + SFLP   E   E P LLY+YRNVMSGFSARLT+E VK+ME+KDGF+SA  ETI++LHT
Sbjct: 61  HRSFLPTSLENSEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHT 120

Query: 121 THTPDYLGLNQQFGLWKNSNFGKGVIIGVLDTGIHPSHPSFSDEGMSPPPAKWKGRCEFN 180
           TH+P++LGLN+QFG WK+SNFGKGVIIGVLD GI PSHPSF D GM  PPAKWKGRCEFN
Sbjct: 121 THSPNFLGLNRQFGFWKDSNFGKGVIIGVLDGGITPSHPSFVDAGMPQPPAKWKGRCEFN 180

Query: 181 ISVCNNKLIGARNFNRGNSFLMG-----DQSPSDEKGHGTHTASTAAGAFVEGAKALGNA 240
            S CNNKLIGAR+ N  +  L G     D SP DE GHGTHTASTAAG FV+GA+ALGNA
Sbjct: 181 FSACNNKLIGARSLNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNA 240

Query: 241 RGKAAGMAPLAHRAIYKVCSVKSCPSSDVLAGIDAAIDDGVDVLSISLGSRSVPFFKDNI 300
            G A GMAPLAH AIYKVC  +SC + D+LAG+DAA++DGVDVLSISLG   VPFF D  
Sbjct: 241 FGTAVGMAPLAHLAIYKVCFGESCSNVDILAGLDAAVEDGVDVLSISLGGPPVPFFADIT 300

Query: 301 AVATFGAIQKGVFVSCSAGNSGPFNSTLSNEAPWILTVEASTINRRIVAVAKLVN----- 360
           A+  F AIQKG+FVSCSA NSGPFN+TLSNEAPWILTV ASTI+R+I A AKL N     
Sbjct: 301 AIGAFAAIQKGIFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFD 360

Query: 361 ---------------------EKTKTLHFVVKVHLK-IWMK---------GGVGRVAKGL 420
                                EK +T+    +  LK I +K         GG+ R+AKG+
Sbjct: 361 GESLFQPNDFPQTFLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCDRGGGIARIAKGV 420

Query: 421 VVKNAGGAAMILINQQQDGFSTLSEAHVLPATHVSYEAGLLIKSYINSSQNPTASISFKG 480
            VKNAGGAAMIL+N + DGF+T ++AHVLPA+HVS+ A L IK+YINS+  PTA+I FKG
Sbjct: 421 EVKNAGGAAMILLNAESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPTATIVFKG 480

Query: 481 TIIGDNDDFSAPAMASFSSRGPCLPSPGILKPDITGPGVNILAAWPFPLDNNTNTKSTFN 509
           T IG  DDFS PA+A+FSSRGP L SPGILKPDITGPGV+ILAAWPFPLDNNTNTKSTFN
Sbjct: 481 TTIG--DDFS-PAIAAFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFN 535

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
SBT17_ARATH2.1e-10444.97Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana GN=SBT1.7 PE=1 SV=1[more]
SBT14_ARATH8.1e-9641.14Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana GN=SBT1.4 PE=2 SV=1[more]
SBT12_ARATH6.2e-8838.64Subtilisin-like protease SBT1.2 OS=Arabidopsis thaliana GN=SBT1.2 PE=2 SV=1[more]
SBT54_ARATH1.7e-8237.08Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana GN=SBT5.4 PE=1 SV=1[more]
SBT19_ARATH4.9e-7737.93Subtilisin-like protease SBT1.9 OS=Arabidopsis thaliana GN=SBT1.9 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KMQ7_CUCSA3.0e-19067.89Uncharacterized protein OS=Cucumis sativus GN=Csa_5G157280 PE=4 SV=1[more]
A0A0A0KKE3_CUCSA3.8e-17762.66Uncharacterized protein OS=Cucumis sativus GN=Csa_5G157240 PE=4 SV=1[more]
B9RBY5_RICCO1.4e-16859.67Cucumisin, putative OS=Ricinus communis GN=RCOM_1682320 PE=4 SV=1[more]
B9GW37_POPTR4.2e-16859.06Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0003s11870g PE=4 SV=2[more]
A0A067JNI9_JATCU1.4e-16658.15Uncharacterized protein OS=Jatropha curcas GN=JCGZ_20707 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G67360.11.2e-10544.97 Subtilase family protein[more]
AT3G14067.14.5e-9741.14 Subtilase family protein[more]
AT1G04110.13.5e-8938.64 Subtilase family protein[more]
AT5G59810.19.8e-8437.08 Subtilase family protein[more]
AT5G67090.12.8e-7837.93 Subtilisin-like serine endopeptidase family protein[more]
Match NameE-valueIdentityDescription
gi|659074655|ref|XP_008437722.1|2.3e-23980.19PREDICTED: subtilisin-like protease [Cucumis melo][more]
gi|659073650|ref|XP_008437177.1|3.6e-19766.97PREDICTED: subtilisin-like protease [Cucumis melo][more]
gi|449459730|ref|XP_004147599.1|4.3e-19067.89PREDICTED: subtilisin-like protease SBT1.7 [Cucumis sativus][more]
gi|659073656|ref|XP_008437181.1|4.2e-17762.48PREDICTED: subtilisin-like protease [Cucumis melo][more]
gi|449459724|ref|XP_004147596.1|5.5e-17762.66PREDICTED: subtilisin-like protease SBT1.2 [Cucumis sativus][more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: Molecular Function
TermDefinition
GO:0004252serine-type endopeptidase activity
Vocabulary: INTERPRO
TermDefinition
IPR023827Peptidase_S8_Asp-AS
IPR015500Peptidase_S8_subtilisin-rel
IPR010259S8pro/Inhibitor_I9
IPR003137PA_domain
IPR000209Peptidase_S8/S53_dom
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005618 cell wall
cellular_component GO:0016020 membrane
molecular_function GO:0004252 serine-type endopeptidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi09G003410.1Lsi09G003410.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000209Peptidase S8/S53 domainGENE3DG3DSA:3.40.50.200coord: 449..508
score: 2.3E-62coord: 134..348
score: 2.3
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 142..508
score: 1.7
IPR000209Peptidase S8/S53 domainunknownSSF52743Subtilisin-likecoord: 453..508
score: 6.68E-59coord: 118..356
score: 6.68
IPR003137PA domainPFAMPF02225PAcoord: 361..419
score: 6.
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 36..119
score: 1.7
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 142..161
score: 1.6E-12coord: 502..510
score: 1.6E-12coord: 209..222
score: 1.6
IPR015500Peptidase S8, subtilisin-relatedPANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 1..508
score: 4.7E
IPR023827Peptidase S8, subtilisin, Asp-active sitePROSITEPS00136SUBTILASE_ASPcoord: 147..158
scor
NoneNo IPR availablePANTHERPTHR10795:SF370SUBFAMILY NOT NAMEDcoord: 1..508
score: 4.7E