Lsi09G003370 (gene) Bottle gourd (USVL1VR-Ls)

BLAST of Lsi09G003370 vs. Swiss-Prot
Match: SBT12_ARATH (Subtilisin-like protease SBT1.2 OS=Arabidopsis thaliana GN=SBT1.2 PE=2 SV=1)

HSP 1 Score: 576.2 bits (1484), Expect = 5.0e-163
Identity = 336/751 (44.74%), Postives = 454/751 (60.45%), Query Frame = 1

Query: 31  RTYIVHVN-KPETTDDLES---WHRSFLPTST---ENSEEQPM--LLYSYRNVMSGFAAR 90
           +TYIV ++   ET     S   WH SFL  +    E  EE+P   LLYSY + + GFAA+
Sbjct: 26  QTYIVQLHPNSETAKTFASKFDWHLSFLQEAVLGVEEEEEEPSSRLLYSYGSAIEGFAAQ 85

Query: 91  LTEEQVKAMEEKDGFVSARRERILNLHTTHTPDFLGLNR--QFGFWKDSNFGKGVIIGVL 150
           LTE + + +      V+ R + +L + TT++  FLGL+     G W  S FG+G IIGVL
Sbjct: 86  LTESEAEILRYSPEVVAVRPDHVLQVQTTYSYKFLGLDGFGNSGVWSKSRFGQGTIIGVL 145

Query: 151 DGGITPNHPSFDDVGMPQPPAKWKGRCE----FNFSACNNKLIGARSFNLASKALKGKTT 210
           D G+ P  PSFDD GMP  P KWKG C+    F+ S+CN KLIGAR F    +       
Sbjct: 146 DTGVWPESPSFDDTGMPSIPRKWKGICQEGESFSSSSCNRKLIGARFFIRGHRVANSPEE 205

Query: 211 TMDD-----SPIDEDGHGTHTASTAAGTFVDGAEALGNAVGTAVGMAPLAHLAIYKVCFG 270
           + +      S  D  GHGTHTAST  G+ V  A  LGN  G A GMAP AH+A+YKVC+ 
Sbjct: 206 SPNMPREYISARDSTGHGTHTASTVGGSSVSMANVLGNGAGVARGMAPGAHIAVYKVCWF 265

Query: 271 EDCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFADNIAIGALAAIQKGIFVSCSAANS 330
             C  +DILAA+D A++D VDVLSLSLG    P + D IAIG   A+++GI V C+A N+
Sbjct: 266 NGCYSSDILAAIDVAIQDKVDVLSLSLGGFPIPLYDDTIAIGTFRAMERGISVICAAGNN 325

Query: 331 GPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDGESLFQ----PNDFPQTFLPL 390
           GP  ++++N APW+ T+ A T+DRR  A  +L NG+   GESL+      N   +  +  
Sbjct: 326 GPIESSVANTAPWVSTIGAGTLDRRFPAVVRLANGKLLYGESLYPGKGIKNAGREVEVIY 385

Query: 391 VFPGEKNETVALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMILLNLES 450
           V  G+K      C  GSL   +++GK+V+C+RG    R  KG  VK AGG AMIL N E 
Sbjct: 386 VTGGDKGS--EFCLRGSLPREEIRGKMVICDRGVN-GRSEKGEAVKEAGGVAMILANTEI 445

Query: 451 DGFTTEADAHVLPASHVSHTVALKIKAYINSTTYPIATIVFKGTIIGDDFSPAIASFSSR 510
           +      D H+LPA+ + +T ++ +KAY+N+T  P A I+F GT+IG   +P +A FS+R
Sbjct: 446 NQEEDSIDVHLLPATLIGYTESVLLKAYVNATVKPKARIIFGGTVIGRSRAPEVAQFSAR 505

Query: 511 GPSLASPGILKPDITGPGVSILAAWP-------FPLDNNTNTKSTFNLVSGTSMSCPHLS 570
           GPSLA+P ILKPD+  PGV+I+AAWP        P D+    +  F ++SGTSMSCPH+S
Sbjct: 506 GPSLANPSILKPDMIAPGVNIIAAWPQNLGPTGLPYDSR---RVNFTVMSGTSMSCPHVS 565

Query: 571 GIAALIKSSHPDWSPAVIKSAIMTTANITNLEGNPILDQTLEPADLFAIGAGHVNPSKAT 630
           GI ALI+S++P+WSPA IKSA+MTTA++ + +G  I D   +PA +FAIGAGHVNP KA 
Sbjct: 566 GITALIRSAYPNWSPAAIKSALMTTADLYDRQGKAIKDGN-KPAGVFAIGAGHVNPQKAI 625

Query: 631 DPGLVYDIQPDDYIPYLCGLGYKNNQVSLIAHKPIDCL-TTPSIPEGELNYPSFTVELGL 690
           +PGLVY+IQP DYI YLC LG+  + +  I HK + C       P   LNYPS  V    
Sbjct: 626 NPGLVYNIQPVDYITYLCTLGFTRSDILAITHKNVSCNGILRKNPGFSLNYPSIAVIFKR 685

Query: 691 PQT---FTRTVTNVGDDCEVYTAVIEAPAAVSVTVRPRKLIFSTLNQKATYSVTF--KRI 742
            +T    TR VTNVG    +Y+  ++AP  + V V P++L+F  ++Q  +Y V F  K+ 
Sbjct: 686 GKTTEMITRRVTNVGSPNSIYSVNVKAPEGIKVIVNPKRLVFKHVDQTLSYRVWFVLKKK 745

BLAST of Lsi09G003370 vs. Swiss-Prot
Match: SBT18_ARATH (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana GN=SBT1.8 PE=2 SV=1)

HSP 1 Score: 568.5 bits (1464), Expect = 1.0e-160
Identity = 325/734 (44.28%), Postives = 450/734 (61.31%), Query Frame = 1

Query: 31  RTYIVHVN---KPETTDDLESWHRSFLPTSTENSEEQPMLLYSYRNVMSGFAARLTEEQV 90
           +TYI+ VN   KPE+      W+     TS  NSE    LLY+Y     GF+A L   + 
Sbjct: 28  KTYIIRVNHSDKPESFLTHHDWY-----TSQLNSESS--LLYTYTTSFHGFSAYLDSTEA 87

Query: 91  KAM-EEKDGFVSARRERILNLHTTHTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGITPN 150
            ++    +  +    + +  LHTT TP+FLGLN +FG     +   GVIIGVLD G+ P 
Sbjct: 88  DSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPE 147

Query: 151 HPSFDDVGMPQPPAKWKGRCE----FNFSACNNKLIGARSFNLASKALKG---KTTTMDD 210
             SFDD  MP+ P+KWKG CE    F+   CN KLIGARSF+   +   G    +     
Sbjct: 148 SRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESV 207

Query: 211 SPIDEDGHGTHTASTAAGTFVDGAEALGNAVGTAVGMAPLAHLAIYKVCFGEDCPDTDIL 270
           SP D DGHGTHT++TAAG+ V  A  LG A GTA GMA  A +A YKVC+   C  +DIL
Sbjct: 208 SPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDIL 267

Query: 271 AALDAAVEDGVDVLSLSLGSPSAPFFADNIAIGALAAIQKGIFVSCSAANSGPFNATLSN 330
           AA+D A+ DGVDVLSLSLG  SAP++ D IAIGA +A+++G+FVSCSA NSGP  A+++N
Sbjct: 268 AAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASVAN 327

Query: 331 EAPWILTVAASTIDRRIVAAAKLGNGEEFDGESLFQPNDFPQTFLPLVFPGEKNETVALC 390
            APW++TV A T+DR   A A LGNG+   G SL+         L LV+    + +  LC
Sbjct: 328 VAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYNKGNSSSSNLC 387

Query: 391 GEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMILLNLESDGFTTEADAHVLP 450
             GSL +  V+GK+VVC+RG   AR+ KG  V++AGG  MI+ N  + G    AD+H+LP
Sbjct: 388 LPGSLDSSIVRGKIVVCDRGVN-ARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLP 447

Query: 451 ASHVSHTVALKIKAYINSTTYPIATIVFKGTIIGDDFSPAIASFSSRGPSLASPGILKPD 510
           A  V       ++ Y+ S + P A +VFKGT++    SP +A+FSSRGP+  +P ILKPD
Sbjct: 448 AIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPD 507

Query: 511 ITGPGVSILAAW-----PFPLDNNTNTKSTFNLVSGTSMSCPHLSGIAALIKSSHPDWSP 570
           + GPGV+ILA W     P  LD ++  ++ FN++SGTSMSCPH+SG+A L+K++HP+WSP
Sbjct: 508 VIGPGVNILAGWSDAIGPTGLDKDSR-RTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSP 567

Query: 571 AVIKSAIMTTANITNLEGNPILDQTLEP-ADLFAIGAGHVNPSKATDPGLVYDIQPDDYI 630
           + IKSA+MTTA + +    P+ D      ++ +A G+GHV+P KA  PGLVYDI  ++YI
Sbjct: 568 SAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYI 627

Query: 631 PYLCGLGYKNNQVSLIAHKP-IDCLTTPSIPEGELNYPSFTVELGLPQT--FTRTVTNVG 690
            +LC L Y  + +  I  +P ++C    S P G+LNYPSF+V  G  +   +TR VTNVG
Sbjct: 628 RFLCSLDYTVDHIVAIVKRPSVNCSKKFSDP-GQLNYPSFSVLFGGKRVVRYTREVTNVG 687

Query: 691 DDCEVYTAVIEAPAAVSVTVRPRKLIFSTLNQKATYSVTFKRIGSISPSIEFAKGYLKWV 745
               VY   +    +V ++V+P KL F ++ +K  Y+VTF     +S + +   G + W 
Sbjct: 688 AASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFGSITWS 747

BLAST of Lsi09G003370 vs. Swiss-Prot
Match: SBT17_ARATH (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 566.6 bits (1459), Expect = 4.0e-160
Identity = 333/759 (43.87%), Postives = 461/759 (60.74%), Query Frame = 1

Query: 6   SLFLLLLLNFHGYVALVTEFPLSSLRTYIVHVNK---PETTDDLESWHRSFLPTSTENSE 65
           + FLLL L F            S   TYIVH+ K   P + D   +W+ S L + ++++E
Sbjct: 10  AFFLLLCLGF-----CHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAE 69

Query: 66  EQPMLLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARRERILNLHTTHTPDFLGLNRQF 125
               LLY+Y N + GF+ RLT+E+  ++  + G +S   E    LHTT TP FLGL+   
Sbjct: 70  ----LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHT 129

Query: 126 G-FWKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPAKWKGRCE----FNFSACNNKL 185
              + ++     V++GVLD G+ P   S+ D G    P+ WKG CE    F  S CN KL
Sbjct: 130 ADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKL 189

Query: 186 IGARSFNLASKALKGKTTTMDDS--PIDEDGHGTHTASTAAGTFVDGAEALGNAVGTAVG 245
           IGAR F    ++  G      +S  P D+DGHGTHT+STAAG+ V+GA  LG A GTA G
Sbjct: 190 IGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARG 249

Query: 246 MAPLAHLAIYKVCFGEDCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFADNIAIGALA 305
           MAP A +A+YKVC+   C  +DILAA+D A+ D V+VLS+SLG   + ++ D +AIGA A
Sbjct: 250 MAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFA 309

Query: 306 AIQKGIFVSCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDGESLFQ 365
           A+++GI VSCSA N+GP +++LSN APWI TV A T+DR   A A LGNG+ F G SLF+
Sbjct: 310 AMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFK 369

Query: 366 PNDFPQTFLPLVFPGEKNETV--ALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKN 425
               P   LP ++ G  +      LC  G+L    VKGK+V+C+RG   AR+ KG  VK 
Sbjct: 370 GEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGIN-ARVQKGDVVKA 429

Query: 426 AGGAAMILLNLESDGFTTEADAHVLPASHVSHTVALKIKAYINSTTYPIATIVFKGTIIG 485
           AGG  MIL N  ++G    ADAH+LPA+ V       I+ Y+ +   P A+I   GT++G
Sbjct: 430 AGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVG 489

Query: 486 DDFSPAIASFSSRGPSLASPGILKPDITGPGVSILAAW-----PFPLDNNTNTKSTFNLV 545
              SP +A+FSSRGP+  +P ILKPD+  PGV+ILAAW     P  L +++  +  FN++
Sbjct: 490 VKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSR-RVEFNII 549

Query: 546 SGTSMSCPHLSGIAALIKSSHPDWSPAVIKSAIMTTANITNLEGNPILD-QTLEPADLFA 605
           SGTSMSCPH+SG+AAL+KS HP+WSPA I+SA+MTTA  T  +G P+LD  T +P+  F 
Sbjct: 550 SGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFD 609

Query: 606 IGAGHVNPSKATDPGLVYDIQPDDYIPYLCGLGYKNNQVSLIAHKPIDCLTTPSIPEGEL 665
            GAGHV+P+ AT+PGL+YD+  +DY+ +LC L Y + Q+  ++ +   C  + S    +L
Sbjct: 610 HGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADL 669

Query: 666 NYPSFTVEL-GL-PQTFTRTVTNVGDDCEVYTAVIEAPAAVSVTVRPRKLIFSTLNQKAT 725
           NYPSF V + G+    +TRTVT+VG        V      V ++V P  L F   N+K +
Sbjct: 670 NYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKS 729

Query: 726 YSVTFKRIGSISPSIEFAKGYLKWVSTKHVVGSPISFKF 745
           Y+VTF  + S  PS   + G ++W   KHVVGSP++  +
Sbjct: 730 YTVTF-TVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of Lsi09G003370 vs. Swiss-Prot
Match: SBT14_ARATH (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 562.4 bits (1448), Expect = 7.5e-159
Identity = 340/748 (45.45%), Postives = 448/748 (59.89%), Query Frame = 1

Query: 30  LRTYIVHV---NKPETTDDLESWHRSFLPTSTENSEEQPMLLYSYRNVMSGFAARLTEEQ 89
           L +YIVHV   +KP       +WH S L  S  +S +   LLYSY   + GF+ARL+  Q
Sbjct: 30  LESYIVHVQRSHKPSLFSSHNNWHVSLL-RSLPSSPQPATLLYSYSRAVHGFSARLSPIQ 89

Query: 90  VKAMEEKDGFVSARRERILNLHTTHTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGITPN 149
             A+      +S   ++   +HTTHTP FLG ++  G W +SN+G+ VI+GVLD GI P 
Sbjct: 90  TAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWSNSNYGEDVIVGVLDTGIWPE 149

Query: 150 HPSFDDVGMPQPPAKWKGRCE----FNFSACNNKLIGARSFN----LASKALKGKTTTMD 209
           HPSF D G+   P+ WKG CE    F  S+CN KLIGAR+F           K       
Sbjct: 150 HPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGARAFYRGYLTQRNGTKKHAAKES 209

Query: 210 DSPIDEDGHGTHTASTAAGTFVDGAEALGNAVGTAVGMAPLAHLAIYKVCFGEDCPDTDI 269
            SP D +GHGTHTASTAAG+ V  A     A GTA GMA  A +A YK+C+   C D+DI
Sbjct: 210 RSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMASKARIAAYKICWTGGCYDSDI 269

Query: 270 LAALDAAVEDGVDVLSLSLG-SPSAP-FFADNIAIGALAAIQKGIFVSCSAANSGPFNAT 329
           LAA+D AV DGV V+SLS+G S SAP +  D+IAIGA  A + GI VSCSA NSGP   T
Sbjct: 270 LAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGATRHGIVVSCSAGNSGPNPET 329

Query: 330 LSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDGESLFQPNDFPQTFLPLVFPGEKNETV 389
            +N APWILTV AST+DR   A A  G+G+ F G SL+     P + L LV+ G+     
Sbjct: 330 ATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYAGESLPDSQLSLVYSGDCGS-- 389

Query: 390 ALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMILLNLESDGFTTEADAH 449
            LC  G L +  V+GK+V+C+RGG  AR+ KG  VK AGGA MIL N    G    AD+H
Sbjct: 390 RLCYPGKLNSSLVEGKIVLCDRGGN-ARVEKGSAVKLAGGAGMILANTAESGEELTADSH 449

Query: 450 VLPASHVSHTVALKIKAYINSTTYPIATIVFKGTIIG-DDFSPAIASFSSRGPSLASPGI 509
           ++PA+ V      +I+ YI ++  P A I F GT+IG    SP +A+FSSRGP+  +P I
Sbjct: 450 LVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSPPSPRVAAFSSRGPNHLTPVI 509

Query: 510 LKPDITGPGVSILAAW-----PFPLDNNTNTKSTFNLVSGTSMSCPHLSGIAALIKSSHP 569
           LKPD+  PGV+ILA W     P  LD +   +  FN++SGTSMSCPH+SG+AAL++ +HP
Sbjct: 510 LKPDVIAPGVNILAGWTGMVGPTDLDIDPR-RVQFNIISGTSMSCPHVSGLAALLRKAHP 569

Query: 570 DWSPAVIKSAIMTTANITNLEGNPILD-QTLEPADLFAIGAGHVNPSKATDPGLVYDIQP 629
           DWSPA IKSA++TTA      G PI D  T + ++ F  GAGHV+P+KA +PGLVYDI+ 
Sbjct: 570 DWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFIHGAGHVDPNKALNPGLVYDIEV 629

Query: 630 DDYIPYLCGLGYKNNQVSLIAHKPI---DCLTTPSIPEGELNYPSFTV---ELGLPQTFT 689
            +Y+ +LC +GY+   + +    P     C T+     G+LNYPSF+V     G    + 
Sbjct: 630 KEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDLNYPSFSVVFASTGEVVKYK 689

Query: 690 RTVTNVGDDCE-VYTAVIEAPAAVSVTVRPRKLIFSTLNQKATYSVTFKR------IGSI 745
           R V NVG + + VY   +++PA V + V P KL FS       Y VTFK       +GS+
Sbjct: 690 RVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSV 749

BLAST of Lsi09G003370 vs. Swiss-Prot
Match: SBT15_ARATH (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 4.1e-157
Identity = 339/767 (44.20%), Postives = 450/767 (58.67%), Query Frame = 1

Query: 15  FHGYVALVTEFPLSSLR-----TYIVHVN---KPETTDDLESWHRSFLPTSTENSEEQPM 74
           F+ +  L    P SS       TYIVHV+   KP        W+ S L + T +    P 
Sbjct: 5   FYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSP---PS 64

Query: 75  LLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARRERILNLHTTHTPDFLGLNR--QFGF 134
           ++++Y  V  GF+ARLT +    + +    +S   E++ +LHTT +P+FLGL    + G 
Sbjct: 65  IIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGL 124

Query: 135 WKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPAKWKGRC----EFNFSACNNKLIGA 194
            ++S+FG  ++IGV+D G+ P  PSFDD G+   P KWKG+C    +F  SACN KL+GA
Sbjct: 125 LEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGA 184

Query: 195 RSFNLASKALKGKT--TTMDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAVGTAVGMAP 254
           R F    +A  GK   TT   SP D DGHGTHTAS +AG +V  A  LG A G A GMAP
Sbjct: 185 RFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAP 244

Query: 255 LAHLAIYKVCFGEDCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFADNIAIGALAAIQ 314
            A LA YKVC+   C D+DILAA D AV DGVDV+SLS+G    P++ D IAIGA  AI 
Sbjct: 245 KARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAID 304

Query: 315 KGIFVSCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDGESLF-QPN 374
           +GIFVS SA N GP   T++N APW+ TV A TIDR   A  KLGNG+   G S++  P 
Sbjct: 305 RGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPG 364

Query: 375 DFPQTFLPLVFPGE----KNETVALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKN 434
             P    PLV+ G        + +LC EGSL    VKGK+V+C+RG   +R  KG  V+ 
Sbjct: 365 LDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGIN-SRATKGEIVRK 424

Query: 435 AGGAAMILLNLESDGFTTEADAHVLPASHVSHTVALKIKAYIN------STTYPIATIVF 494
            GG  MI+ N   DG    AD HVLPA+ V  +   +I+ YI+      S+ +P ATIVF
Sbjct: 425 NGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVF 484

Query: 495 KGTIIGDDFSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLD----NNTNTKS 554
           KGT +G   +P +ASFS+RGP+  +P ILKPD+  PG++ILAAWP  +      + N ++
Sbjct: 485 KGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRT 544

Query: 555 TFNLVSGTSMSCPHLSGIAALIKSSHPDWSPAVIKSAIMTTANITNLEGNPILDQ-TLEP 614
            FN++SGTSM+CPH+SG+AAL+K++HPDWSPA I+SA++TTA   +  G P++D+ T   
Sbjct: 545 EFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNT 604

Query: 615 ADLFAIGAGHVNPSKATDPGLVYDIQPDDYIPYLCGLGYKNNQVSLIAHKPIDCLTTPSI 674
           + +   G+GHV+P+KA DPGLVYDI   DYI +LC   Y    +  I  +  DC      
Sbjct: 605 SSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRA 664

Query: 675 PE-GELNYPSFTV------ELGLPQTFTRTVTNVGDDCEVYTAVIEAPAAVSVTVRPRKL 734
              G LNYPSF+V      E  +   F RTVTNVGD   VY   I  P   +VTV P KL
Sbjct: 665 GHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKL 724

Query: 735 IFSTLNQKATYSVTFKRIG-SISP-SIEFAKGYLKWVSTKHVVGSPI 741
            F  + QK ++ V  K     +SP +     G++ W   K  V SP+
Sbjct: 725 SFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPL 767

BLAST of Lsi09G003370 vs. TrEMBL
Match: A0A0A0KKE3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G157240 PE=4 SV=1)

HSP 1 Score: 1323.5 bits (3424), Expect = 0.0e+00
Identity = 666/745 (89.40%), Postives = 690/745 (92.62%), Query Frame = 1

Query: 1   MVLLPSLFLLLLLNFHGYVALVTEFPLSSLRTYIVHVNKPETTDDLESWHRSFLPTSTEN 60
           MVLLPSLFLLLLLNFH Y A VTE P S+L TYIVHV KPE  DDLESWHRSFLPTS EN
Sbjct: 1   MVLLPSLFLLLLLNFHVYEAQVTELPFSNLHTYIVHVKKPEVVDDLESWHRSFLPTSLEN 60

Query: 61  SEEQPMLLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARRERILNLHTTHTPDFLGLNR 120
           SEEQP LLYSYRNVMSGF+ARLTEE VKAMEEKDGFVSARRE I++LHTTH+P+FLGLNR
Sbjct: 61  SEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHSPNFLGLNR 120

Query: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPAKWKGRCEFNFSACNNKLIGA 180
           QFGFWKDSNFGKGVIIGVLDGGITP+HPSF D GMPQPPAKWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGITPSHPSFVDAGMPQPPAKWKGRCEFNFSACNNKLIGA 180

Query: 181 RSFNLASKALKGKTTTMDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAVGTAVGMAPLA 240
           RS NLAS+ALKGK TT+DDSPIDEDGHGTHTASTAAGTFVDGAEALGNA GTAVGMAPLA
Sbjct: 181 RSLNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240

Query: 241 HLAIYKVCFGEDCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFADNIAIGALAAIQKG 300
           HLAIYKVCFGE C + DILA LDAAVEDGVDVLS+SLG P  PFFAD  AIGA AAIQKG
Sbjct: 241 HLAIYKVCFGESCSNVDILAGLDAAVEDGVDVLSISLGGPPVPFFADITAIGAFAAIQKG 300

Query: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDGESLFQPNDFP 360
           IFVSCSAANSGPFNATLSNEAPWILTVAASTIDR+I A AKLGNGEEFDGESLFQPNDFP
Sbjct: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360

Query: 361 QTFLPLVFPGEKNETVALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMI 420
           QTFLPLVFPGEKNETVALC EGSLKNIDVKGKVVVC+RGGGIARIAKGVEVKNAGGAAMI
Sbjct: 361 QTFLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCDRGGGIARIAKGVEVKNAGGAAMI 420

Query: 421 LLNLESDGFTTEADAHVLPASHVSHTVALKIKAYINSTTYPIATIVFKGTIIGDDFSPAI 480
           LLN ESDGFTTEADAHVLPASHVSHT ALKIKAYINSTTYP ATIVFKGT IGDDFSPAI
Sbjct: 421 LLNAESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPTATIVFKGTTIGDDFSPAI 480

Query: 481 ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNLVSGTSMSCPHLSG 540
           A+FSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFN+VSGTSMSCPHLSG
Sbjct: 481 AAFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIVSGTSMSCPHLSG 540

Query: 541 IAALIKSSHPDWSPAVIKSAIMTTANITNLEGNPILDQTLEPADLFAIGAGHVNPSKATD 600
           IAALIKS+HPDWSPA IKS+IMTTANITNLEGNPI+DQTL+PADLFAIGAGHVNPSKA D
Sbjct: 541 IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPIVDQTLQPADLFAIGAGHVNPSKAVD 600

Query: 601 PGLVYDIQPDDYIPYLCGLGYKNNQVSLIAHKPIDCLTTPSIPEGELNYPSFTVELGLPQ 660
           PGLVYDIQPDDYIPYLCGLGY NNQVSLIAHKPIDCLTT SIPEGELNYPSF V+LG  Q
Sbjct: 601 PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTTSIPEGELNYPSFMVKLGQVQ 660

Query: 661 TFTRTVTNVGDDCEVYTAVIEAPAAVSVTVRPRKLIFSTLNQKATYSVTFKRIGSISPSI 720
           TF+RTVT VG   EVY  VIEAP  VSVTVRPRK+IFS LNQKATYSVTFKRIGSISPS 
Sbjct: 661 TFSRTVTYVGSGREVYNVVIEAPEGVSVTVRPRKVIFSALNQKATYSVTFKRIGSISPST 720

Query: 721 EFAKGYLKWVSTKHVVGSPISFKFV 746
           EFA+GYLKWVS KH+V SPIS KFV
Sbjct: 721 EFAEGYLKWVSAKHLVRSPISVKFV 745

BLAST of Lsi09G003370 vs. TrEMBL
Match: B9GW37_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0003s11870g PE=4 SV=2)

HSP 1 Score: 1030.0 bits (2662), Expect = 1.4e-297
Identity = 526/755 (69.67%), Postives = 606/755 (80.26%), Query Frame = 1

Query: 1   MVLLPSLFLLLLLNFHGYVALVTEFPL-----SSLRTYIVHVNKPETT-----DDLESWH 60
           M ++P L L+  LNF   +A  T+        ++L  YIVHV KPE       +DLESW+
Sbjct: 1   MAIIPLLLLIFKLNFCPEIAQATQHTTKITEKTTLLNYIVHVAKPEGRTMAEFEDLESWY 60

Query: 61  RSFLPTSTENSEEQPMLLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARRERILNLHTT 120
           +SFLP ST +SE+Q  +LY+Y+NVMSGFAARLT+E+VK+MEEKDGF+SAR ERIL+L TT
Sbjct: 61  QSFLPVSTASSEKQQRMLYAYQNVMSGFAARLTQEEVKSMEEKDGFLSARPERILHLQTT 120

Query: 121 HTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPAKWKGRCEFNF 180
           HTP FLGL+++ GFWK+SNFGKGVIIGVLDGGI P+HPSF D GMP PPAKWKGRC+FN 
Sbjct: 121 HTPRFLGLHQELGFWKESNFGKGVIIGVLDGGIFPSHPSFSDEGMPPPPAKWKGRCDFNA 180

Query: 181 SACNNKLIGARSFNLASKALKGKTTTMDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAV 240
           S CNNKLIGARSFN+A+KA KG   T  + PID DGHGTHTASTAAG FV  AE LGNA 
Sbjct: 181 SDCNNKLIGARSFNIAAKAKKGSAAT--EPPIDVDGHGTHTASTAAGAFVKDAEVLGNAR 240

Query: 241 GTAVGMAPLAHLAIYKVCFGE---DCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFAD 300
           GTAVG+AP AHLAIYKVCFG+   DCP++DILA LDAAV+DGVDVLSLSLG  S P F D
Sbjct: 241 GTAVGIAPHAHLAIYKVCFGDPGDDCPESDILAGLDAAVQDGVDVLSLSLGEDSVPLFND 300

Query: 301 NIAIGALAAIQKGIFVSCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEE 360
            IAIG+ AAIQKGIFVSCSA NSGPFN TLSNEAPWILTV AST+DRR  A A+LGNGE+
Sbjct: 301 TIAIGSFAAIQKGIFVSCSAGNSGPFNGTLSNEAPWILTVGASTVDRRFSATARLGNGEQ 360

Query: 361 FDGESLFQPNDFPQTFLPLVFPGEKNE-TVALCGEGSLKNIDVKGKVVVCERGGGIARIA 420
            DGESL Q ++FP T LPLV+ G   +   +LCGEG+L+ +DVKGK+V+CERGGGI RIA
Sbjct: 361 IDGESLSQHSNFPSTLLPLVYAGMSGKPNSSLCGEGALEGMDVKGKIVLCERGGGIGRIA 420

Query: 421 KGVEVKNAGGAAMILLNLESDGFTTEADAHVLPASHVSHTVALKIKAYINSTTYPIATIV 480
           KG EVKNAGGAAMIL+N E+DGF+T AD HVLPA+HVS    LKIKAYINST  P+ATI+
Sbjct: 421 KGGEVKNAGGAAMILMNEEADGFSTNADVHVLPATHVSFAAGLKIKAYINSTQAPMATIL 480

Query: 481 FKGTIIGDDFSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFN 540
           FKGT+IGD  SP +ASFSSRGPSLASPGILKPDI GPGVSILAAWPFPLDNNTN+KSTFN
Sbjct: 481 FKGTVIGDSSSPFVASFSSRGPSLASPGILKPDIIGPGVSILAAWPFPLDNNTNSKSTFN 540

Query: 541 LVSGTSMSCPHLSGIAALIKSSHPDWSPAVIKSAIMTTANITNLEGNPILDQTLEPADLF 600
           ++SGTSMSCPHLSGIAAL+KSSHP WSPA IKSAIMTTA+  N+EG  I+DQTL+PAD+F
Sbjct: 541 IISGTSMSCPHLSGIAALLKSSHPYWSPAAIKSAIMTTADTLNMEGKLIVDQTLQPADIF 600

Query: 601 AIGAGHVNPSKATDPGLVYDIQPDDYIPYLCGLGYKNNQVSLIAHKPIDCLTTPSIPEGE 660
           A GAGHVNPS+A +PGLVYDIQPDDYIPYLCGLGY +N+VS+I H+ + C   PSIPEGE
Sbjct: 601 ATGAGHVNPSRANNPGLVYDIQPDDYIPYLCGLGYADNEVSIIVHEQVKCSEKPSIPEGE 660

Query: 661 LNYPSFTVELGLPQTFTRTVTNVGDDCEVYTAVIEAPAAVSVTVRPRKLIFSTLNQKATY 720
           LNYPSF V LG  QTFTRTVTNVGD    Y   I +P  V VTV+P KL FS +NQKATY
Sbjct: 661 LNYPSFAVTLGPSQTFTRTVTNVGDVNSAYEVAIVSPPGVDVTVKPSKLYFSKVNQKATY 720

Query: 721 SVTFKRIGSISPSIEFAKGYLKWVSTKHVVGSPIS 742
           SV F R      + E A+GY+ W S K+ V SPI+
Sbjct: 721 SVAFSRTEYGGKTSETAQGYIVWASAKYTVRSPIA 753

BLAST of Lsi09G003370 vs. TrEMBL
Match: A0A067JNI9_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_20707 PE=4 SV=1)

HSP 1 Score: 1028.9 bits (2659), Expect = 3.1e-297
Identity = 525/759 (69.17%), Postives = 609/759 (80.24%), Query Frame = 1

Query: 1   MVLLPSLFLLLLLNFHGYVALVTEFPLS-----SLRTYIVHVNKPE-----TTDDLESWH 60
           M  +P L ++ L NF+  +A   +         +L+ YIVHV+ PE       ++LE+WH
Sbjct: 1   MAAIPFLIVIFLFNFYPNIAQAAKHTTEITENKNLQAYIVHVSPPEGRTFSQRENLENWH 60

Query: 61  RSFLPTSTENSE-EQPMLLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARRERILNLHT 120
           +SFLP ST +SE +Q  +LYSY N++SGF+ARLT E+VKAMEE +GFV AR ER L+L T
Sbjct: 61  KSFLPFSTASSEKQQKRMLYSYHNIISGFSARLTHEEVKAMEEINGFVLARPERKLHLQT 120

Query: 121 THTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPAKWKGRCEFN 180
           THTP FLGL+RQ GFWK+SNFGKGVIIGVLDGG+ P+HPSF+D GMP PPAKWKGRCEFN
Sbjct: 121 THTPSFLGLHRQMGFWKESNFGKGVIIGVLDGGVFPSHPSFNDKGMPPPPAKWKGRCEFN 180

Query: 181 FSACNNKLIGARSFNLASKALKGKTTTMDDSPIDEDGHGTHTASTAAGTFVDGAEALGNA 240
            S CNNKLIGARSFNLA+KA+KG      ++PID DGHGTHTASTAAG+FV  A  LGNA
Sbjct: 181 ASKCNNKLIGARSFNLAAKAMKGIAA---ETPIDVDGHGTHTASTAAGSFVYNANVLGNA 240

Query: 241 VGTAVGMAPLAHLAIYKVCFGE---DCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFA 300
            GTAVGMAP AHLAIYKVCFG+   DCP++DILA LDAA++DGVDVLSLS+G  S PFF 
Sbjct: 241 KGTAVGMAPYAHLAIYKVCFGDPNDDCPESDILAGLDAAIQDGVDVLSLSIGDISMPFFQ 300

Query: 301 DNIAIGALAAIQKGIFVSCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGE 360
           DNIAIG+ AAIQKGIFVSC+A NSGPFN TLSNEAPWILTV ASTIDR+I A AKLGNGE
Sbjct: 301 DNIAIGSFAAIQKGIFVSCAAGNSGPFNGTLSNEAPWILTVGASTIDRKIAATAKLGNGE 360

Query: 361 EFDGESLFQPNDFPQTFLPLVFPGEKNETV-ALCGEGSLKNIDVKGKVVVCERGGGIARI 420
           E DGES+ QP++FP T LPLV+PG   +T  A C E +++ +DVK KVV+CERGGGI R+
Sbjct: 361 ELDGESVLQPSNFPTTLLPLVYPGMNGKTESAFCSERAVQGMDVKDKVVLCERGGGIGRV 420

Query: 421 AKGVEVKNAGGAAMILLNLESDGFTTEADAHVLPASHVSHTVALKIKAYINSTTYPIATI 480
           AKG EVKNAGGAAMIL+N E  GF+T ADAHVLPA+HVS    L+IKAYINST  P+ATI
Sbjct: 421 AKGEEVKNAGGAAMILINDEISGFSTIADAHVLPATHVSFAAGLQIKAYINSTKTPMATI 480

Query: 481 VFKGTIIGDDFSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTF 540
           +FKGT+IGD  SPA+ SFSSRGP+LASPGILKPDI GPGVSILAAWPFPLDN TNTKSTF
Sbjct: 481 LFKGTVIGDPLSPAVTSFSSRGPNLASPGILKPDIIGPGVSILAAWPFPLDNTTNTKSTF 540

Query: 541 NLVSGTSMSCPHLSGIAALIKSSHPDWSPAVIKSAIMTTANITNLEGNPILDQTLEPADL 600
           NLVSGTSM+CPHLSGIAAL+KSSHP WSPA IKSAIMTTA+I N+EG+PI+D+  +PADL
Sbjct: 541 NLVSGTSMACPHLSGIAALLKSSHPYWSPAAIKSAIMTTADIFNMEGSPIVDEKHQPADL 600

Query: 601 FAIGAGHVNPSKATDPGLVYDIQPDDYIPYLCGLGYKNNQVSLIAHKPIDCLTTPSIPEG 660
           F IGAGHVNPS+A DPGL+YDIQPDDYIPYLCGLGYK  QVS+IAH+ I C    SIPEG
Sbjct: 601 FTIGAGHVNPSRANDPGLIYDIQPDDYIPYLCGLGYKEEQVSIIAHRRIKCSEKLSIPEG 660

Query: 661 ELNYPSFTVELGLPQTFTRTVTNVGDDCEVYTAVIEAPAAVSVTVRPRKLIFSTLNQKAT 720
           +LNYPSF+V LG  QTFTRTVTNVG+   VY A I  P  V+VTV P +L FS +NQK T
Sbjct: 661 QLNYPSFSVTLGASQTFTRTVTNVGEANSVYAATIVPPPGVAVTVEPYRLYFSQVNQKVT 720

Query: 721 YSVTFKRIGSISPSIEFAKGYLKWVSTKHVVGSPISFKF 745
           YSVTF   GS   + EFA+GY+ W S KH+V SPIS +F
Sbjct: 721 YSVTFSPTGSSGKTSEFAQGYILWSSAKHLVRSPISVRF 756

BLAST of Lsi09G003370 vs. TrEMBL
Match: W9RYM8_9ROSA (Subtilisin-like protease SDD1 OS=Morus notabilis GN=L484_023157 PE=4 SV=1)

HSP 1 Score: 1025.4 bits (2650), Expect = 3.5e-296
Identity = 528/752 (70.21%), Postives = 600/752 (79.79%), Query Frame = 1

Query: 7   LFLLLLLNFHGYVALVTEF-------PLSSLRTYIVHVNKPE-----TTDDLESWHRSFL 66
           L  + +LNF   +AL +E          SSL+ YI+HV  P+      ++DLESW+RSFL
Sbjct: 91  LSFIFVLNFFHVIALQSEVISVSQTTESSSLQNYIIHVKPPKGRVLSQSEDLESWYRSFL 150

Query: 67  PTSTE-NSEEQPMLLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARRERILNLHTTHTP 126
           P +T  +S+ QP +LY+YRNV+ GFAARLT++QV+AME KDGF+SAR ERIL   TTHTP
Sbjct: 151 PATTAASSDNQPRMLYAYRNVLRGFAARLTQDQVRAMEGKDGFISARPERILKKLTTHTP 210

Query: 127 DFLGLNRQFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPAKWKGRCEFNFSAC 186
           +FLGL++Q GFW+DSNFGKGVIIGVLDGGI P+HPSF D GMP PPAKWKGRC+FN S C
Sbjct: 211 NFLGLHQQKGFWRDSNFGKGVIIGVLDGGIFPSHPSFSDEGMPPPPAKWKGRCDFNVSDC 270

Query: 187 NNKLIGARSFNLASKALKGKTTTMDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAVGTA 246
           NNKLIGARSFNLA+KA KG      + PIDEDGHGTHTASTAAG FV+ A+ LGNA GTA
Sbjct: 271 NNKLIGARSFNLAAKATKGDKA---EPPIDEDGHGTHTASTAAGGFVNYADVLGNAKGTA 330

Query: 247 VGMAPLAHLAIYKVCFGEDCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFADNIAIGA 306
           VGMAP AHLAIYKVCFGEDCPD DILAALDAAVEDGVDVLSLSLG  S PFF D++AIGA
Sbjct: 331 VGMAPYAHLAIYKVCFGEDCPDADILAALDAAVEDGVDVLSLSLGDVSRPFFNDSLAIGA 390

Query: 307 LAAIQKGIFVSCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDGESL 366
            AA +KGI VSCSA NSGP N+TLSNEAPWILTV ASTIDR+I+A AKLGN EEFDGES+
Sbjct: 391 FAATEKGILVSCSAGNSGPVNSTLSNEAPWILTVGASTIDRKIIATAKLGNDEEFDGESI 450

Query: 367 FQPNDFPQTFLPLVFPGEKNET-VALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVK 426
            +  DFPQT  PLV+ G   +   A C EGSLK+IDVK KVV+CERGGG+ RIAKG EVK
Sbjct: 451 HR-GDFPQTSWPLVYAGINGKADSAFCAEGSLKDIDVKNKVVLCERGGGVGRIAKGEEVK 510

Query: 427 NAGGAAMILLNLESDGFTTEADAHVLPASHVSHTVALKIKAYINSTTYPIATIVFKGTII 486
           NAGGAAMIL+N ESDGF+TEAD H LPA+HVS    LKIKAYINST  P AT+ FKGT+I
Sbjct: 511 NAGGAAMILVNQESDGFSTEADPHALPAAHVSFADGLKIKAYINSTATPTATLFFKGTVI 570

Query: 487 GDDFSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNLVSGTS 546
           GD  +P IASFSSRGP+LASPGILKPDI GPGVSILAAWPFPLDNNTN KS FN++SGTS
Sbjct: 571 GDSLAPFIASFSSRGPNLASPGILKPDIIGPGVSILAAWPFPLDNNTNPKSPFNIMSGTS 630

Query: 547 MSCPHLSGIAALIKSSHPDWSPAVIKSAIMTTANITNLEGNPILDQTLEPADLFAIGAGH 606
           MSCPHLSGIA L+KSSHP WSPA IKSAIMTTA+I NLEG  ILDQ L PAD+FA GAGH
Sbjct: 631 MSCPHLSGIAVLLKSSHPYWSPAAIKSAIMTTADIVNLEGKAILDQALTPADVFATGAGH 690

Query: 607 VNPSKATDPGLVYDIQPDDYIPYLCGLGYKNNQVSLIAHKPIDCLTTPSIPEGELNYPSF 666
           VNP KA DPGL+YD+QPDDYIPYLCGLGY + +V ++A +PI C   PSIPEGELNYPSF
Sbjct: 691 VNPIKANDPGLIYDLQPDDYIPYLCGLGYNDKEVGIVARRPIKCSEKPSIPEGELNYPSF 750

Query: 667 TVELGLPQTFTRTVTNVGDDCEVYTAVIEAPAAVSVTVRPRKLIFSTLNQKATYSVTFKR 726
           +V LG  QTFTRTVTNVG+    YTA I AP  V V+V+P KL FS +NQKATYSV F R
Sbjct: 751 SVTLGPSQTFTRTVTNVGEAYSTYTANIMAPDGVYVSVKPSKLYFSKVNQKATYSVNFSR 810

Query: 727 IGSISPSIEFAKGYLKWVSTKHVVGSPISFKF 745
           I S   +  + +G+L WVS +H V SPIS KF
Sbjct: 811 ITSSGETGPYGQGFLTWVSARHCVRSPISVKF 838

BLAST of Lsi09G003370 vs. TrEMBL
Match: B9RBY5_RICCO (Cucumisin, putative OS=Ricinus communis GN=RCOM_1682320 PE=4 SV=1)

HSP 1 Score: 1023.8 bits (2646), Expect = 1.0e-295
Identity = 523/760 (68.82%), Postives = 613/760 (80.66%), Query Frame = 1

Query: 1   MVLLPSLFLLLLLNFHGYVALVTEFPLSS-----LRTYIVHVNKPE-----TTDDLESWH 60
           M ++P +FL+ L NF+  +A   E    +     L+TYIVHVN+PE       +DL++WH
Sbjct: 10  MTVVPFIFLIFLFNFYPLIAQSAEHTTETIEKKNLQTYIVHVNQPEGRTFSQPEDLKNWH 69

Query: 61  RSFLPTSTENSEE--QPMLLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARRERILNLH 120
           +SFL  ST +SEE  Q  +LYSY+N++SGF+ARLT+E+VKAMEE  GFVSA  ER L L 
Sbjct: 70  KSFLSFSTASSEEEQQQRMLYSYQNIISGFSARLTQEEVKAMEEITGFVSACLERKLRLQ 129

Query: 121 TTHTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPAKWKGRCEF 180
           TTHTP FLGL++Q G WKDS+FGKGVIIG+LDGG+ P+HPSF D GMP PPAKWKGRCEF
Sbjct: 130 TTHTPSFLGLHQQMGLWKDSDFGKGVIIGILDGGVYPSHPSFSDEGMPLPPAKWKGRCEF 189

Query: 181 NFSACNNKLIGARSFNLASKALKGKTTTMDDSPIDEDGHGTHTASTAAGTFVDGAEALGN 240
           N S CNNKLIGAR+FNLA+K +KG  T   + PID DGHGTHTASTAAG FV  ++ LGN
Sbjct: 190 NASECNNKLIGARTFNLAAKTMKGAPT---EPPIDVDGHGTHTASTAAGGFVYNSDVLGN 249

Query: 241 AVGTAVGMAPLAHLAIYKVCFGE---DCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFF 300
           A GTAVGMAP AHLAIYKVCFG+   DCP++D+LA LDAAV+DGVDVLSLSLG  S PFF
Sbjct: 250 AKGTAVGMAPFAHLAIYKVCFGDPNDDCPESDVLAGLDAAVDDGVDVLSLSLGDVSMPFF 309

Query: 301 ADNIAIGALAAIQKGIFVSCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNG 360
            DNIAIG+ AAIQKGIFVSCSA NSGP  +TLSNEAPWILTV ASTIDRRIVA AKLGNG
Sbjct: 310 QDNIAIGSFAAIQKGIFVSCSAGNSGPSKSTLSNEAPWILTVGASTIDRRIVAIAKLGNG 369

Query: 361 EEFDGESLFQPNDFPQTFLPLVFPGEKNET-VALCGEGSLKNIDVKGKVVVCERGGGIAR 420
           EE DGES+ QP++FP T LP+V+ G  ++   A CGEG+L+ ++VK KVV+CERGGGI R
Sbjct: 370 EELDGESVSQPSNFPTTLLPIVYAGMNSKPDSAFCGEGALEGMNVKDKVVMCERGGGIGR 429

Query: 421 IAKGVEVKNAGGAAMILLNLESDGFTTEADAHVLPASHVSHTVALKIKAYINSTTYPIAT 480
           IAKG EVKNAGGAAMIL+N E++GF+T ADAHVLPA+HVS    LKIKAYINST  P+AT
Sbjct: 430 IAKGDEVKNAGGAAMILVNDETNGFSTIADAHVLPATHVSFAAGLKIKAYINSTKTPMAT 489

Query: 481 IVFKGTIIGDDFSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKST 540
           I+FKGT+IGD  SPA+ SFSSRGPSLASPGILKPDI GPGVSILAAWPFPLDNNTNTK T
Sbjct: 490 ILFKGTVIGDSSSPAVTSFSSRGPSLASPGILKPDIIGPGVSILAAWPFPLDNNTNTKLT 549

Query: 541 FNLVSGTSMSCPHLSGIAALIKSSHPDWSPAVIKSAIMTTANITNLEGNPILDQTLEPAD 600
           FN++SGTSMSCPHLSGIAAL+KSSHP WSPA IKSAI+TTA+I N+EG PI+D+T +PAD
Sbjct: 550 FNIMSGTSMSCPHLSGIAALLKSSHPYWSPAAIKSAIVTTADILNMEGKPIVDETHQPAD 609

Query: 601 LFAIGAGHVNPSKATDPGLVYDIQPDDYIPYLCGLGYKNNQVSLIAHKPIDCLTTPSIPE 660
            FA GAGHVNPS+A DPGLVYDIQPDDYIPYLCGL Y + QVS+IAH+PI C T  +I E
Sbjct: 610 FFATGAGHVNPSRANDPGLVYDIQPDDYIPYLCGLNYTDEQVSIIAHRPISCSTIQTIAE 669

Query: 661 GELNYPSFTVELGLPQTFTRTVTNVGDDCEVYTAVIEAPAAVSVTVRPRKLIFSTLNQKA 720
           G+LNYPSF+V LG PQTF RTVTNVG    V+ A I +P  V+V+V+P +L FS LNQKA
Sbjct: 670 GQLNYPSFSVTLGPPQTFIRTVTNVGYANSVFAATITSPPGVAVSVKPSRLYFSKLNQKA 729

Query: 721 TYSVTFKRIGSISPSIEFAKGYLKWVSTKHVVGSPISFKF 745
           TYS+TF   G  + + EF +GY+ WVS K+ VGSPIS +F
Sbjct: 730 TYSITFSHTGYGAKTSEFGQGYITWVSDKYFVGSPISVRF 766

BLAST of Lsi09G003370 vs. TAIR10
Match: AT1G04110.1 (AT1G04110.1 Subtilase family protein)

HSP 1 Score: 576.2 bits (1484), Expect = 2.8e-164
Identity = 336/751 (44.74%), Postives = 454/751 (60.45%), Query Frame = 1

Query: 31  RTYIVHVN-KPETTDDLES---WHRSFLPTST---ENSEEQPM--LLYSYRNVMSGFAAR 90
           +TYIV ++   ET     S   WH SFL  +    E  EE+P   LLYSY + + GFAA+
Sbjct: 26  QTYIVQLHPNSETAKTFASKFDWHLSFLQEAVLGVEEEEEEPSSRLLYSYGSAIEGFAAQ 85

Query: 91  LTEEQVKAMEEKDGFVSARRERILNLHTTHTPDFLGLNR--QFGFWKDSNFGKGVIIGVL 150
           LTE + + +      V+ R + +L + TT++  FLGL+     G W  S FG+G IIGVL
Sbjct: 86  LTESEAEILRYSPEVVAVRPDHVLQVQTTYSYKFLGLDGFGNSGVWSKSRFGQGTIIGVL 145

Query: 151 DGGITPNHPSFDDVGMPQPPAKWKGRCE----FNFSACNNKLIGARSFNLASKALKGKTT 210
           D G+ P  PSFDD GMP  P KWKG C+    F+ S+CN KLIGAR F    +       
Sbjct: 146 DTGVWPESPSFDDTGMPSIPRKWKGICQEGESFSSSSCNRKLIGARFFIRGHRVANSPEE 205

Query: 211 TMDD-----SPIDEDGHGTHTASTAAGTFVDGAEALGNAVGTAVGMAPLAHLAIYKVCFG 270
           + +      S  D  GHGTHTAST  G+ V  A  LGN  G A GMAP AH+A+YKVC+ 
Sbjct: 206 SPNMPREYISARDSTGHGTHTASTVGGSSVSMANVLGNGAGVARGMAPGAHIAVYKVCWF 265

Query: 271 EDCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFADNIAIGALAAIQKGIFVSCSAANS 330
             C  +DILAA+D A++D VDVLSLSLG    P + D IAIG   A+++GI V C+A N+
Sbjct: 266 NGCYSSDILAAIDVAIQDKVDVLSLSLGGFPIPLYDDTIAIGTFRAMERGISVICAAGNN 325

Query: 331 GPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDGESLFQ----PNDFPQTFLPL 390
           GP  ++++N APW+ T+ A T+DRR  A  +L NG+   GESL+      N   +  +  
Sbjct: 326 GPIESSVANTAPWVSTIGAGTLDRRFPAVVRLANGKLLYGESLYPGKGIKNAGREVEVIY 385

Query: 391 VFPGEKNETVALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMILLNLES 450
           V  G+K      C  GSL   +++GK+V+C+RG    R  KG  VK AGG AMIL N E 
Sbjct: 386 VTGGDKGS--EFCLRGSLPREEIRGKMVICDRGVN-GRSEKGEAVKEAGGVAMILANTEI 445

Query: 451 DGFTTEADAHVLPASHVSHTVALKIKAYINSTTYPIATIVFKGTIIGDDFSPAIASFSSR 510
           +      D H+LPA+ + +T ++ +KAY+N+T  P A I+F GT+IG   +P +A FS+R
Sbjct: 446 NQEEDSIDVHLLPATLIGYTESVLLKAYVNATVKPKARIIFGGTVIGRSRAPEVAQFSAR 505

Query: 511 GPSLASPGILKPDITGPGVSILAAWP-------FPLDNNTNTKSTFNLVSGTSMSCPHLS 570
           GPSLA+P ILKPD+  PGV+I+AAWP        P D+    +  F ++SGTSMSCPH+S
Sbjct: 506 GPSLANPSILKPDMIAPGVNIIAAWPQNLGPTGLPYDSR---RVNFTVMSGTSMSCPHVS 565

Query: 571 GIAALIKSSHPDWSPAVIKSAIMTTANITNLEGNPILDQTLEPADLFAIGAGHVNPSKAT 630
           GI ALI+S++P+WSPA IKSA+MTTA++ + +G  I D   +PA +FAIGAGHVNP KA 
Sbjct: 566 GITALIRSAYPNWSPAAIKSALMTTADLYDRQGKAIKDGN-KPAGVFAIGAGHVNPQKAI 625

Query: 631 DPGLVYDIQPDDYIPYLCGLGYKNNQVSLIAHKPIDCL-TTPSIPEGELNYPSFTVELGL 690
           +PGLVY+IQP DYI YLC LG+  + +  I HK + C       P   LNYPS  V    
Sbjct: 626 NPGLVYNIQPVDYITYLCTLGFTRSDILAITHKNVSCNGILRKNPGFSLNYPSIAVIFKR 685

Query: 691 PQT---FTRTVTNVGDDCEVYTAVIEAPAAVSVTVRPRKLIFSTLNQKATYSVTF--KRI 742
            +T    TR VTNVG    +Y+  ++AP  + V V P++L+F  ++Q  +Y V F  K+ 
Sbjct: 686 GKTTEMITRRVTNVGSPNSIYSVNVKAPEGIKVIVNPKRLVFKHVDQTLSYRVWFVLKKK 745

BLAST of Lsi09G003370 vs. TAIR10
Match: AT2G05920.1 (AT2G05920.1 Subtilase family protein)

HSP 1 Score: 568.5 bits (1464), Expect = 5.9e-162
Identity = 325/734 (44.28%), Postives = 450/734 (61.31%), Query Frame = 1

Query: 31  RTYIVHVN---KPETTDDLESWHRSFLPTSTENSEEQPMLLYSYRNVMSGFAARLTEEQV 90
           +TYI+ VN   KPE+      W+     TS  NSE    LLY+Y     GF+A L   + 
Sbjct: 28  KTYIIRVNHSDKPESFLTHHDWY-----TSQLNSESS--LLYTYTTSFHGFSAYLDSTEA 87

Query: 91  KAM-EEKDGFVSARRERILNLHTTHTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGITPN 150
            ++    +  +    + +  LHTT TP+FLGLN +FG     +   GVIIGVLD G+ P 
Sbjct: 88  DSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPE 147

Query: 151 HPSFDDVGMPQPPAKWKGRCE----FNFSACNNKLIGARSFNLASKALKG---KTTTMDD 210
             SFDD  MP+ P+KWKG CE    F+   CN KLIGARSF+   +   G    +     
Sbjct: 148 SRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESV 207

Query: 211 SPIDEDGHGTHTASTAAGTFVDGAEALGNAVGTAVGMAPLAHLAIYKVCFGEDCPDTDIL 270
           SP D DGHGTHT++TAAG+ V  A  LG A GTA GMA  A +A YKVC+   C  +DIL
Sbjct: 208 SPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDIL 267

Query: 271 AALDAAVEDGVDVLSLSLGSPSAPFFADNIAIGALAAIQKGIFVSCSAANSGPFNATLSN 330
           AA+D A+ DGVDVLSLSLG  SAP++ D IAIGA +A+++G+FVSCSA NSGP  A+++N
Sbjct: 268 AAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASVAN 327

Query: 331 EAPWILTVAASTIDRRIVAAAKLGNGEEFDGESLFQPNDFPQTFLPLVFPGEKNETVALC 390
            APW++TV A T+DR   A A LGNG+   G SL+         L LV+    + +  LC
Sbjct: 328 VAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYNKGNSSSSNLC 387

Query: 391 GEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMILLNLESDGFTTEADAHVLP 450
             GSL +  V+GK+VVC+RG   AR+ KG  V++AGG  MI+ N  + G    AD+H+LP
Sbjct: 388 LPGSLDSSIVRGKIVVCDRGVN-ARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLP 447

Query: 451 ASHVSHTVALKIKAYINSTTYPIATIVFKGTIIGDDFSPAIASFSSRGPSLASPGILKPD 510
           A  V       ++ Y+ S + P A +VFKGT++    SP +A+FSSRGP+  +P ILKPD
Sbjct: 448 AIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPD 507

Query: 511 ITGPGVSILAAW-----PFPLDNNTNTKSTFNLVSGTSMSCPHLSGIAALIKSSHPDWSP 570
           + GPGV+ILA W     P  LD ++  ++ FN++SGTSMSCPH+SG+A L+K++HP+WSP
Sbjct: 508 VIGPGVNILAGWSDAIGPTGLDKDSR-RTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSP 567

Query: 571 AVIKSAIMTTANITNLEGNPILDQTLEP-ADLFAIGAGHVNPSKATDPGLVYDIQPDDYI 630
           + IKSA+MTTA + +    P+ D      ++ +A G+GHV+P KA  PGLVYDI  ++YI
Sbjct: 568 SAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYI 627

Query: 631 PYLCGLGYKNNQVSLIAHKP-IDCLTTPSIPEGELNYPSFTVELGLPQT--FTRTVTNVG 690
            +LC L Y  + +  I  +P ++C    S P G+LNYPSF+V  G  +   +TR VTNVG
Sbjct: 628 RFLCSLDYTVDHIVAIVKRPSVNCSKKFSDP-GQLNYPSFSVLFGGKRVVRYTREVTNVG 687

Query: 691 DDCEVYTAVIEAPAAVSVTVRPRKLIFSTLNQKATYSVTFKRIGSISPSIEFAKGYLKWV 745
               VY   +    +V ++V+P KL F ++ +K  Y+VTF     +S + +   G + W 
Sbjct: 688 AASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFGSITWS 747

BLAST of Lsi09G003370 vs. TAIR10
Match: AT5G67360.1 (AT5G67360.1 Subtilase family protein)

HSP 1 Score: 566.6 bits (1459), Expect = 2.2e-161
Identity = 333/759 (43.87%), Postives = 461/759 (60.74%), Query Frame = 1

Query: 6   SLFLLLLLNFHGYVALVTEFPLSSLRTYIVHVNK---PETTDDLESWHRSFLPTSTENSE 65
           + FLLL L F            S   TYIVH+ K   P + D   +W+ S L + ++++E
Sbjct: 10  AFFLLLCLGF-----CHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAE 69

Query: 66  EQPMLLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARRERILNLHTTHTPDFLGLNRQF 125
               LLY+Y N + GF+ RLT+E+  ++  + G +S   E    LHTT TP FLGL+   
Sbjct: 70  ----LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHT 129

Query: 126 G-FWKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPAKWKGRCE----FNFSACNNKL 185
              + ++     V++GVLD G+ P   S+ D G    P+ WKG CE    F  S CN KL
Sbjct: 130 ADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKL 189

Query: 186 IGARSFNLASKALKGKTTTMDDS--PIDEDGHGTHTASTAAGTFVDGAEALGNAVGTAVG 245
           IGAR F    ++  G      +S  P D+DGHGTHT+STAAG+ V+GA  LG A GTA G
Sbjct: 190 IGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARG 249

Query: 246 MAPLAHLAIYKVCFGEDCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFADNIAIGALA 305
           MAP A +A+YKVC+   C  +DILAA+D A+ D V+VLS+SLG   + ++ D +AIGA A
Sbjct: 250 MAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFA 309

Query: 306 AIQKGIFVSCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDGESLFQ 365
           A+++GI VSCSA N+GP +++LSN APWI TV A T+DR   A A LGNG+ F G SLF+
Sbjct: 310 AMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFK 369

Query: 366 PNDFPQTFLPLVFPGEKNETV--ALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKN 425
               P   LP ++ G  +      LC  G+L    VKGK+V+C+RG   AR+ KG  VK 
Sbjct: 370 GEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGIN-ARVQKGDVVKA 429

Query: 426 AGGAAMILLNLESDGFTTEADAHVLPASHVSHTVALKIKAYINSTTYPIATIVFKGTIIG 485
           AGG  MIL N  ++G    ADAH+LPA+ V       I+ Y+ +   P A+I   GT++G
Sbjct: 430 AGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVG 489

Query: 486 DDFSPAIASFSSRGPSLASPGILKPDITGPGVSILAAW-----PFPLDNNTNTKSTFNLV 545
              SP +A+FSSRGP+  +P ILKPD+  PGV+ILAAW     P  L +++  +  FN++
Sbjct: 490 VKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSR-RVEFNII 549

Query: 546 SGTSMSCPHLSGIAALIKSSHPDWSPAVIKSAIMTTANITNLEGNPILD-QTLEPADLFA 605
           SGTSMSCPH+SG+AAL+KS HP+WSPA I+SA+MTTA  T  +G P+LD  T +P+  F 
Sbjct: 550 SGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFD 609

Query: 606 IGAGHVNPSKATDPGLVYDIQPDDYIPYLCGLGYKNNQVSLIAHKPIDCLTTPSIPEGEL 665
            GAGHV+P+ AT+PGL+YD+  +DY+ +LC L Y + Q+  ++ +   C  + S    +L
Sbjct: 610 HGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADL 669

Query: 666 NYPSFTVEL-GL-PQTFTRTVTNVGDDCEVYTAVIEAPAAVSVTVRPRKLIFSTLNQKAT 725
           NYPSF V + G+    +TRTVT+VG        V      V ++V P  L F   N+K +
Sbjct: 670 NYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKS 729

Query: 726 YSVTFKRIGSISPSIEFAKGYLKWVSTKHVVGSPISFKF 745
           Y+VTF  + S  PS   + G ++W   KHVVGSP++  +
Sbjct: 730 YTVTF-TVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of Lsi09G003370 vs. TAIR10
Match: AT3G14067.1 (AT3G14067.1 Subtilase family protein)

HSP 1 Score: 562.4 bits (1448), Expect = 4.2e-160
Identity = 340/748 (45.45%), Postives = 448/748 (59.89%), Query Frame = 1

Query: 30  LRTYIVHV---NKPETTDDLESWHRSFLPTSTENSEEQPMLLYSYRNVMSGFAARLTEEQ 89
           L +YIVHV   +KP       +WH S L  S  +S +   LLYSY   + GF+ARL+  Q
Sbjct: 30  LESYIVHVQRSHKPSLFSSHNNWHVSLL-RSLPSSPQPATLLYSYSRAVHGFSARLSPIQ 89

Query: 90  VKAMEEKDGFVSARRERILNLHTTHTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGITPN 149
             A+      +S   ++   +HTTHTP FLG ++  G W +SN+G+ VI+GVLD GI P 
Sbjct: 90  TAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWSNSNYGEDVIVGVLDTGIWPE 149

Query: 150 HPSFDDVGMPQPPAKWKGRCE----FNFSACNNKLIGARSFN----LASKALKGKTTTMD 209
           HPSF D G+   P+ WKG CE    F  S+CN KLIGAR+F           K       
Sbjct: 150 HPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGARAFYRGYLTQRNGTKKHAAKES 209

Query: 210 DSPIDEDGHGTHTASTAAGTFVDGAEALGNAVGTAVGMAPLAHLAIYKVCFGEDCPDTDI 269
            SP D +GHGTHTASTAAG+ V  A     A GTA GMA  A +A YK+C+   C D+DI
Sbjct: 210 RSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMASKARIAAYKICWTGGCYDSDI 269

Query: 270 LAALDAAVEDGVDVLSLSLG-SPSAP-FFADNIAIGALAAIQKGIFVSCSAANSGPFNAT 329
           LAA+D AV DGV V+SLS+G S SAP +  D+IAIGA  A + GI VSCSA NSGP   T
Sbjct: 270 LAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGATRHGIVVSCSAGNSGPNPET 329

Query: 330 LSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDGESLFQPNDFPQTFLPLVFPGEKNETV 389
            +N APWILTV AST+DR   A A  G+G+ F G SL+     P + L LV+ G+     
Sbjct: 330 ATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYAGESLPDSQLSLVYSGDCGS-- 389

Query: 390 ALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMILLNLESDGFTTEADAH 449
            LC  G L +  V+GK+V+C+RGG  AR+ KG  VK AGGA MIL N    G    AD+H
Sbjct: 390 RLCYPGKLNSSLVEGKIVLCDRGGN-ARVEKGSAVKLAGGAGMILANTAESGEELTADSH 449

Query: 450 VLPASHVSHTVALKIKAYINSTTYPIATIVFKGTIIG-DDFSPAIASFSSRGPSLASPGI 509
           ++PA+ V      +I+ YI ++  P A I F GT+IG    SP +A+FSSRGP+  +P I
Sbjct: 450 LVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSPPSPRVAAFSSRGPNHLTPVI 509

Query: 510 LKPDITGPGVSILAAW-----PFPLDNNTNTKSTFNLVSGTSMSCPHLSGIAALIKSSHP 569
           LKPD+  PGV+ILA W     P  LD +   +  FN++SGTSMSCPH+SG+AAL++ +HP
Sbjct: 510 LKPDVIAPGVNILAGWTGMVGPTDLDIDPR-RVQFNIISGTSMSCPHVSGLAALLRKAHP 569

Query: 570 DWSPAVIKSAIMTTANITNLEGNPILD-QTLEPADLFAIGAGHVNPSKATDPGLVYDIQP 629
           DWSPA IKSA++TTA      G PI D  T + ++ F  GAGHV+P+KA +PGLVYDI+ 
Sbjct: 570 DWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFIHGAGHVDPNKALNPGLVYDIEV 629

Query: 630 DDYIPYLCGLGYKNNQVSLIAHKPI---DCLTTPSIPEGELNYPSFTV---ELGLPQTFT 689
            +Y+ +LC +GY+   + +    P     C T+     G+LNYPSF+V     G    + 
Sbjct: 630 KEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDLNYPSFSVVFASTGEVVKYK 689

Query: 690 RTVTNVGDDCE-VYTAVIEAPAAVSVTVRPRKLIFSTLNQKATYSVTFKR------IGSI 745
           R V NVG + + VY   +++PA V + V P KL FS       Y VTFK       +GS+
Sbjct: 690 RVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSV 749

BLAST of Lsi09G003370 vs. TAIR10
Match: AT3G14240.1 (AT3G14240.1 Subtilase family protein)

HSP 1 Score: 556.6 bits (1433), Expect = 2.3e-158
Identity = 339/767 (44.20%), Postives = 450/767 (58.67%), Query Frame = 1

Query: 15  FHGYVALVTEFPLSSLR-----TYIVHVN---KPETTDDLESWHRSFLPTSTENSEEQPM 74
           F+ +  L    P SS       TYIVHV+   KP        W+ S L + T +    P 
Sbjct: 5   FYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSP---PS 64

Query: 75  LLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARRERILNLHTTHTPDFLGLNR--QFGF 134
           ++++Y  V  GF+ARLT +    + +    +S   E++ +LHTT +P+FLGL    + G 
Sbjct: 65  IIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGL 124

Query: 135 WKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPAKWKGRC----EFNFSACNNKLIGA 194
            ++S+FG  ++IGV+D G+ P  PSFDD G+   P KWKG+C    +F  SACN KL+GA
Sbjct: 125 LEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGA 184

Query: 195 RSFNLASKALKGKT--TTMDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAVGTAVGMAP 254
           R F    +A  GK   TT   SP D DGHGTHTAS +AG +V  A  LG A G A GMAP
Sbjct: 185 RFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAP 244

Query: 255 LAHLAIYKVCFGEDCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFADNIAIGALAAIQ 314
            A LA YKVC+   C D+DILAA D AV DGVDV+SLS+G    P++ D IAIGA  AI 
Sbjct: 245 KARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAID 304

Query: 315 KGIFVSCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDGESLF-QPN 374
           +GIFVS SA N GP   T++N APW+ TV A TIDR   A  KLGNG+   G S++  P 
Sbjct: 305 RGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPG 364

Query: 375 DFPQTFLPLVFPGE----KNETVALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKN 434
             P    PLV+ G        + +LC EGSL    VKGK+V+C+RG   +R  KG  V+ 
Sbjct: 365 LDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGIN-SRATKGEIVRK 424

Query: 435 AGGAAMILLNLESDGFTTEADAHVLPASHVSHTVALKIKAYIN------STTYPIATIVF 494
            GG  MI+ N   DG    AD HVLPA+ V  +   +I+ YI+      S+ +P ATIVF
Sbjct: 425 NGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVF 484

Query: 495 KGTIIGDDFSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLD----NNTNTKS 554
           KGT +G   +P +ASFS+RGP+  +P ILKPD+  PG++ILAAWP  +      + N ++
Sbjct: 485 KGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRT 544

Query: 555 TFNLVSGTSMSCPHLSGIAALIKSSHPDWSPAVIKSAIMTTANITNLEGNPILDQ-TLEP 614
            FN++SGTSM+CPH+SG+AAL+K++HPDWSPA I+SA++TTA   +  G P++D+ T   
Sbjct: 545 EFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNT 604

Query: 615 ADLFAIGAGHVNPSKATDPGLVYDIQPDDYIPYLCGLGYKNNQVSLIAHKPIDCLTTPSI 674
           + +   G+GHV+P+KA DPGLVYDI   DYI +LC   Y    +  I  +  DC      
Sbjct: 605 SSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRA 664

Query: 675 PE-GELNYPSFTV------ELGLPQTFTRTVTNVGDDCEVYTAVIEAPAAVSVTVRPRKL 734
              G LNYPSF+V      E  +   F RTVTNVGD   VY   I  P   +VTV P KL
Sbjct: 665 GHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKL 724

Query: 735 IFSTLNQKATYSVTFKRIG-SISP-SIEFAKGYLKWVSTKHVVGSPI 741
            F  + QK ++ V  K     +SP +     G++ W   K  V SP+
Sbjct: 725 SFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPL 767

BLAST of Lsi09G003370 vs. NCBI nr
Match: gi|659073656|ref|XP_008437181.1| (PREDICTED: subtilisin-like protease [Cucumis melo])

HSP 1 Score: 1327.0 bits (3433), Expect = 0.0e+00
Identity = 667/745 (89.53%), Postives = 688/745 (92.35%), Query Frame = 1

Query: 1   MVLLPSLFLLLLLNFHGYVALVTEFPLSSLRTYIVHVNKPETTDDLESWHRSFLPTSTEN 60
           MVLLPSLFLLLLLNFHGY A VTE PLS+L TYIVHV KPE  DDLE WHRSFLPTS +N
Sbjct: 1   MVLLPSLFLLLLLNFHGYEAQVTELPLSNLHTYIVHVKKPEVVDDLEIWHRSFLPTSLDN 60

Query: 61  SEEQPMLLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARRERILNLHTTHTPDFLGLNR 120
            EEQP LLYSYRNVMSGF+ARLTEE VKAMEEKDGFVSARRE I++LHTTHTPDFLGLNR
Sbjct: 61  EEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGLNR 120

Query: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPAKWKGRCEFNFSACNNKLIGA 180
           QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDD GM QPPAKWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMAQPPAKWKGRCEFNFSACNNKLIGA 180

Query: 181 RSFNLASKALKGKTTTMDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAVGTAVGMAPLA 240
           RS NLAS+ALKGK TT+DDSPIDEDGHGTHTASTAAGTFVDGAEALGNA GTAVGMAPLA
Sbjct: 181 RSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240

Query: 241 HLAIYKVCFGEDCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFADNIAIGALAAIQKG 300
           HLAIYKVCFGEDC D DILA LDAAVEDGVDVLS+SLG PS PFFAD  AIG+ AAIQKG
Sbjct: 241 HLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQKG 300

Query: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDGESLFQPNDFP 360
           IFVSCSAANSGPFNATLSNEAPWILTVAASTIDR+I A AKLGNGEEFDGESLFQPNDFP
Sbjct: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360

Query: 361 QTFLPLVFPGEKNETVALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMI 420
           QT LPLVFPGEKNETVALC EGSLKNIDVKGKVVVCERGGGIARIAKGVEVKN GGAAMI
Sbjct: 361 QTLLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMI 420

Query: 421 LLNLESDGFTTEADAHVLPASHVSHTVALKIKAYINSTTYPIATIVFKGTIIGDDFSPAI 480
           LLN ESDGFTTE DAHVLPASHVSHT ALKIKAYINSTTYP ATI+FKGT IGDDFSPAI
Sbjct: 421 LLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGTTIGDDFSPAI 480

Query: 481 ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNLVSGTSMSCPHLSG 540
           ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFN++SGTSMSCPHLSG
Sbjct: 481 ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISGTSMSCPHLSG 540

Query: 541 IAALIKSSHPDWSPAVIKSAIMTTANITNLEGNPILDQTLEPADLFAIGAGHVNPSKATD 600
           IAALIKS+HPDWSPA IKS+IMTTANITNLEGNPILD+TL+PADLFAIGAGHVNPSKA D
Sbjct: 541 IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILDETLQPADLFAIGAGHVNPSKAID 600

Query: 601 PGLVYDIQPDDYIPYLCGLGYKNNQVSLIAHKPIDCLTTPSIPEGELNYPSFTVELGLPQ 660
           PGLVYDIQPDDYIPYLCGLGY NNQVSLIAHKPIDCLTT SIPEGELNYPSF V+LG  Q
Sbjct: 601 PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYPSFMVKLGPVQ 660

Query: 661 TFTRTVTNVGDDCEVYTAVIEAPAAVSVTVRPRKLIFSTLNQKATYSVTFKRIGSISPSI 720
           TF+RTVT+VG    VY  VIEAP  VSVTVRPRKL FS LNQKATYSVTFKR GSISPSI
Sbjct: 661 TFSRTVTSVGSGRVVYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTFKRSGSISPSI 720

Query: 721 EFAKGYLKWVSTKHVVGSPISFKFV 746
           EFA+GYLKWVS KHVV SPIS KFV
Sbjct: 721 EFAEGYLKWVSAKHVVRSPISVKFV 745

BLAST of Lsi09G003370 vs. NCBI nr
Match: gi|449459724|ref|XP_004147596.1| (PREDICTED: subtilisin-like protease SBT1.2 [Cucumis sativus])

HSP 1 Score: 1323.5 bits (3424), Expect = 0.0e+00
Identity = 666/745 (89.40%), Postives = 690/745 (92.62%), Query Frame = 1

Query: 1   MVLLPSLFLLLLLNFHGYVALVTEFPLSSLRTYIVHVNKPETTDDLESWHRSFLPTSTEN 60
           MVLLPSLFLLLLLNFH Y A VTE P S+L TYIVHV KPE  DDLESWHRSFLPTS EN
Sbjct: 1   MVLLPSLFLLLLLNFHVYEAQVTELPFSNLHTYIVHVKKPEVVDDLESWHRSFLPTSLEN 60

Query: 61  SEEQPMLLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARRERILNLHTTHTPDFLGLNR 120
           SEEQP LLYSYRNVMSGF+ARLTEE VKAMEEKDGFVSARRE I++LHTTH+P+FLGLNR
Sbjct: 61  SEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHSPNFLGLNR 120

Query: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPAKWKGRCEFNFSACNNKLIGA 180
           QFGFWKDSNFGKGVIIGVLDGGITP+HPSF D GMPQPPAKWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGITPSHPSFVDAGMPQPPAKWKGRCEFNFSACNNKLIGA 180

Query: 181 RSFNLASKALKGKTTTMDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAVGTAVGMAPLA 240
           RS NLAS+ALKGK TT+DDSPIDEDGHGTHTASTAAGTFVDGAEALGNA GTAVGMAPLA
Sbjct: 181 RSLNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240

Query: 241 HLAIYKVCFGEDCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFADNIAIGALAAIQKG 300
           HLAIYKVCFGE C + DILA LDAAVEDGVDVLS+SLG P  PFFAD  AIGA AAIQKG
Sbjct: 241 HLAIYKVCFGESCSNVDILAGLDAAVEDGVDVLSISLGGPPVPFFADITAIGAFAAIQKG 300

Query: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDGESLFQPNDFP 360
           IFVSCSAANSGPFNATLSNEAPWILTVAASTIDR+I A AKLGNGEEFDGESLFQPNDFP
Sbjct: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360

Query: 361 QTFLPLVFPGEKNETVALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMI 420
           QTFLPLVFPGEKNETVALC EGSLKNIDVKGKVVVC+RGGGIARIAKGVEVKNAGGAAMI
Sbjct: 361 QTFLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCDRGGGIARIAKGVEVKNAGGAAMI 420

Query: 421 LLNLESDGFTTEADAHVLPASHVSHTVALKIKAYINSTTYPIATIVFKGTIIGDDFSPAI 480
           LLN ESDGFTTEADAHVLPASHVSHT ALKIKAYINSTTYP ATIVFKGT IGDDFSPAI
Sbjct: 421 LLNAESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPTATIVFKGTTIGDDFSPAI 480

Query: 481 ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNLVSGTSMSCPHLSG 540
           A+FSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFN+VSGTSMSCPHLSG
Sbjct: 481 AAFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIVSGTSMSCPHLSG 540

Query: 541 IAALIKSSHPDWSPAVIKSAIMTTANITNLEGNPILDQTLEPADLFAIGAGHVNPSKATD 600
           IAALIKS+HPDWSPA IKS+IMTTANITNLEGNPI+DQTL+PADLFAIGAGHVNPSKA D
Sbjct: 541 IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPIVDQTLQPADLFAIGAGHVNPSKAVD 600

Query: 601 PGLVYDIQPDDYIPYLCGLGYKNNQVSLIAHKPIDCLTTPSIPEGELNYPSFTVELGLPQ 660
           PGLVYDIQPDDYIPYLCGLGY NNQVSLIAHKPIDCLTT SIPEGELNYPSF V+LG  Q
Sbjct: 601 PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTTSIPEGELNYPSFMVKLGQVQ 660

Query: 661 TFTRTVTNVGDDCEVYTAVIEAPAAVSVTVRPRKLIFSTLNQKATYSVTFKRIGSISPSI 720
           TF+RTVT VG   EVY  VIEAP  VSVTVRPRK+IFS LNQKATYSVTFKRIGSISPS 
Sbjct: 661 TFSRTVTYVGSGREVYNVVIEAPEGVSVTVRPRKVIFSALNQKATYSVTFKRIGSISPST 720

Query: 721 EFAKGYLKWVSTKHVVGSPISFKFV 746
           EFA+GYLKWVS KH+V SPIS KFV
Sbjct: 721 EFAEGYLKWVSAKHLVRSPISVKFV 745

BLAST of Lsi09G003370 vs. NCBI nr
Match: gi|731379755|ref|XP_002275471.2| (PREDICTED: uncharacterized protein LOC100242816 [Vitis vinifera])

HSP 1 Score: 1035.4 bits (2676), Expect = 4.8e-299
Identity = 514/723 (71.09%), Postives = 592/723 (81.88%), Query Frame = 1

Query: 28   SSLRTYIVHVNKPETT-----DDLESWHRSFLPTSTENSEEQPMLLYSYRNVMSGFAARL 87
            S L+TYIVHV + E +     ++LESWHRSFLP +T  S+ Q  L+YSY+NV+SGFAARL
Sbjct: 765  SMLQTYIVHVKQLERSTTAQQENLESWHRSFLPVATATSDNQERLVYSYKNVISGFAARL 824

Query: 88   TEEQVKAMEEKDGFVSARRERILNLHTTHTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGG 147
            TEE+V+AME  DGF+SA  E++L L TTH+PDFLGL+++ GFWK+SNFGKGVIIGVLD G
Sbjct: 825  TEEEVRAMENMDGFISASPEKMLPLLTTHSPDFLGLHQEMGFWKESNFGKGVIIGVLDSG 884

Query: 148  ITPNHPSFDDVGMPQPPAKWKGRCEFNFSACNNKLIGARSFNLASKALKGKTTTMDDSPI 207
            + P+HPSF   G+P PPAKWKG CEF  S CNNKLIGARSFN+ +KA KG T    + P+
Sbjct: 885  VLPSHPSFSGEGIPPPPAKWKGSCEFMASECNNKLIGARSFNVGAKATKGVTA---EPPL 944

Query: 208  DEDGHGTHTASTAAGTFVDGAEALGNAVGTAVGMAPLAHLAIYKVCFGEDCPDTDILAAL 267
            D+DGHGTHTASTAAG FV  A+ LGNA GTAVGMAP AHLAIYKVCFG DCP++D++A L
Sbjct: 945  DDDGHGTHTASTAAGAFVKNADVLGNAKGTAVGMAPYAHLAIYKVCFGPDCPESDVIAGL 1004

Query: 268  DAAVEDGVDVLSLSLGSPSAPFFADNIAIGALAAIQKGIFVSCSAANSGPFNATLSNEAP 327
            DAAVEDGVDV+S+SLG P+ PFF DNIA+G+ AA+QKGIFVSCSA NSGPFN TLSNEAP
Sbjct: 1005 DAAVEDGVDVISISLGDPAVPFFQDNIAVGSFAAMQKGIFVSCSAGNSGPFNTTLSNEAP 1064

Query: 328  WILTVAASTIDRRIVAAAKLGNGEEFDGESLFQPNDFPQTFLPLVFPGEKNETV-ALCGE 387
            WILTV AS+IDR I AAAKLGNGE+FDGE+LFQP+DFP T LPLV+ G   +   A+CGE
Sbjct: 1065 WILTVGASSIDRTIKAAAKLGNGEQFDGETLFQPSDFPATQLPLVYAGMNGKPESAVCGE 1124

Query: 388  GSLKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMILLNLESDGFTTEADAHVLPAS 447
            GSLKNIDVKGKVV+C+RGGGIARI KG EVKNAGGAAMIL+N ESDGF+T ADAHVLPA+
Sbjct: 1125 GSLKNIDVKGKVVLCDRGGGIARIDKGTEVKNAGGAAMILVNQESDGFSTLADAHVLPAT 1184

Query: 448  HVSHTVALKIKAYINSTTYPIATIVFKGTIIGDDFSPAIASFSSRGPSLASPGILKPDIT 507
            HVS+   LKIKAYINST  P A I+FKGT+IG+  SPAI SFSSRGPS ASPGILKPDI 
Sbjct: 1185 HVSYAAGLKIKAYINSTATPTAAILFKGTVIGNPLSPAITSFSSRGPSFASPGILKPDII 1244

Query: 508  GPGVSILAAWPFPLDNNTNTKSTFNLVSGTSMSCPHLSGIAALIKSSHPDWSPAVIKSAI 567
            GPGVSILAAWPFPLDNN N+KSTFN++SGTSMSCPHLSGIAAL+KSSHPDWSPA IKSAI
Sbjct: 1245 GPGVSILAAWPFPLDNNINSKSTFNIISGTSMSCPHLSGIAALLKSSHPDWSPAAIKSAI 1304

Query: 568  MTTANITNLEGNPILDQTLEPADLFAIGAGHVNPSKATDPGLVYDIQPDDYIPYLCGLGY 627
            MTTA++ N+ G PI+D+ L PAD+FA GAGHVNPS+A DPGLVYDI+PDDYIPYLCGLGY
Sbjct: 1305 MTTADLLNVGGKPIVDERLLPADIFATGAGHVNPSRANDPGLVYDIEPDDYIPYLCGLGY 1364

Query: 628  KNNQVSLIAHKPIDCLTTPSIPEGELNYPSFTVELGLPQTFTRTVTNVGDDCEVYTAVIE 687
             + +V ++AH+ I C    SIPEGELNYPSF+V LG PQTFTRTVTNVG+    YT    
Sbjct: 1365 TDTEVGILAHRSIKCSEESSIPEGELNYPSFSVALGPPQTFTRTVTNVGEAYSSYTVTAI 1424

Query: 688  APAAVSVTVRPRKLIFSTLNQKATYSVTFKRIGSISPSIEFAKGYLKWVSTKHVVGSPIS 745
             P  V V+V P KL FS +NQK TYSVTF    S   S +FA+GYLKWVS KH VGSPIS
Sbjct: 1425 VPQGVDVSVNPDKLYFSKVNQKLTYSVTFSHNSSSGKSSKFAQGYLKWVSGKHSVGSPIS 1484

BLAST of Lsi09G003370 vs. NCBI nr
Match: gi|470129682|ref|XP_004300738.1| (PREDICTED: subtilisin-like protease SDD1 [Fragaria vesca subsp. vesca])

HSP 1 Score: 1031.2 bits (2665), Expect = 9.1e-298
Identity = 518/747 (69.34%), Postives = 602/747 (80.59%), Query Frame = 1

Query: 1   MVLLPSLFLLLLLNFHGYVALVTEFPLSSLRTYIVHVNKPE-----TTDDLESWHRSFLP 60
           M ++P L L+ +L+F   +A  TE  +S L+TYIVHV +PE      T+DLESWH+SFLP
Sbjct: 1   MAVIPFLTLIFMLSFSHGIAQKTE--ISPLQTYIVHVMQPEGRVFAATEDLESWHKSFLP 60

Query: 61  TSTENSEEQPMLLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARRERILNLHTTHTPDF 120
           + T +S++Q  LLYSY+ V+SGF+ARLT+E+VK ME  DGFV+A  ER+    TTHTP+F
Sbjct: 61  SMTASSDDQTRLLYSYKTVISGFSARLTQEEVKVMELMDGFVAAHPERVFRRKTTHTPNF 120

Query: 121 LGLNRQFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPAKWKGRCEFNFSACNN 180
           LGLNRQ G WKDSNFGKGVIIGVLDGG+ P+HPSF   G+P PPAKWKGRC+FN S CNN
Sbjct: 121 LGLNRQAGIWKDSNFGKGVIIGVLDGGVFPSHPSFSGAGIPPPPAKWKGRCDFNVSECNN 180

Query: 181 KLIGARSFNLASKALKGKTTTMDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAVGTAVG 240
           KLIGA+SFNLA+ ALKG      + PIDEDGHGTHTASTA G FV  A+ LGNA GTAVG
Sbjct: 181 KLIGAQSFNLAAMALKGAKA---EPPIDEDGHGTHTASTAGGAFVQNADVLGNAKGTAVG 240

Query: 241 MAPLAHLAIYKVCFGEDCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFADNIAIGALA 300
           MAP AHLAIYKVCFGE CP++DILAAL+AAV DGVDV+S+SLG  S PFF D+ AIG+ A
Sbjct: 241 MAPYAHLAIYKVCFGEPCPESDILAALEAAVHDGVDVISISLGEDSVPFFQDSTAIGSFA 300

Query: 301 AIQKGIFVSCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDGESLFQ 360
           AIQKGIFVSC+A NSGPFN T+SNEAPWILTV AST+DRRIVA+A LGNG  FDGESLFQ
Sbjct: 301 AIQKGIFVSCAAGNSGPFNGTISNEAPWILTVGASTLDRRIVASAALGNGLVFDGESLFQ 360

Query: 361 PNDFPQTFLPLVFPGEKNETV-ALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNA 420
           P DFP T LPLV+ G   +   A C EGSLKNI VKGKVVVCERGGGI RI KGVEVKNA
Sbjct: 361 PKDFPSTLLPLVYAGVIGKVESAFCAEGSLKNISVKGKVVVCERGGGIGRIDKGVEVKNA 420

Query: 421 GGAAMILLNLESDGFTTEADAHVLPASHVSHTVALKIKAYINSTTYPIATIVFKGTIIGD 480
           GGAAMILLN E+DGF+T ADAHVLPA+HV+H   L IKAYINST  P ATI+FKGT+IGD
Sbjct: 421 GGAAMILLNAETDGFSTSADAHVLPAAHVTHAAGLNIKAYINSTATPTATILFKGTVIGD 480

Query: 481 DFSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNLVSGTSMS 540
             SPA+ASFSSRGP+LASPGILKPDI GPGV++LAAWPFPLDNNT   STFN++SGTSMS
Sbjct: 481 STSPAVASFSSRGPNLASPGILKPDIIGPGVNVLAAWPFPLDNNTKAASTFNIISGTSMS 540

Query: 541 CPHLSGIAALIKSSHPDWSPAVIKSAIMTTANITNLEGNPILDQTLEPADLFAIGAGHVN 600
           CPHLSGIAAL+KSSHP WSPA IKSAIMT+A++ NL+G  I D+TL+PAD+ A GAGHVN
Sbjct: 541 CPHLSGIAALLKSSHPYWSPAAIKSAIMTSADLINLQGKLIFDETLQPADVLATGAGHVN 600

Query: 601 PSKATDPGLVYDIQPDDYIPYLCGLGYKNNQVSLIAHKPIDCLTTPSIPEGELNYPSFTV 660
           PSKATDPGLVYDIQPDDYIPYLCGLGYK+++VS++AH+PI C    SIPEGELNYPSF+V
Sbjct: 601 PSKATDPGLVYDIQPDDYIPYLCGLGYKDSEVSILAHRPITCSKVSSIPEGELNYPSFSV 660

Query: 661 ELGLPQTFTRTVTNVGDDCEVYTAVIEAPAAVSVTVRPRKLIFSTLNQKATYSVTFKRIG 720
           +LG  QTFTRTVTNVG     Y+  + AP  V VTV+P  L F+ +NQK +YSVTF   G
Sbjct: 661 KLGPSQTFTRTVTNVGAPYSTYSVKVNAPQGVYVTVKPSTLYFTKMNQKMSYSVTFSH-G 720

Query: 721 SISPSIEFAKGYLKWVSTKHVVGSPIS 742
           S   +  F +G++ W S KH+V SP+S
Sbjct: 721 SGGKAGSFTQGFITWASAKHIVRSPVS 741

BLAST of Lsi09G003370 vs. NCBI nr
Match: gi|566162226|ref|XP_002303551.2| (hypothetical protein POPTR_0003s11870g [Populus trichocarpa])

HSP 1 Score: 1030.0 bits (2662), Expect = 2.0e-297
Identity = 526/755 (69.67%), Postives = 606/755 (80.26%), Query Frame = 1

Query: 1   MVLLPSLFLLLLLNFHGYVALVTEFPL-----SSLRTYIVHVNKPETT-----DDLESWH 60
           M ++P L L+  LNF   +A  T+        ++L  YIVHV KPE       +DLESW+
Sbjct: 1   MAIIPLLLLIFKLNFCPEIAQATQHTTKITEKTTLLNYIVHVAKPEGRTMAEFEDLESWY 60

Query: 61  RSFLPTSTENSEEQPMLLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARRERILNLHTT 120
           +SFLP ST +SE+Q  +LY+Y+NVMSGFAARLT+E+VK+MEEKDGF+SAR ERIL+L TT
Sbjct: 61  QSFLPVSTASSEKQQRMLYAYQNVMSGFAARLTQEEVKSMEEKDGFLSARPERILHLQTT 120

Query: 121 HTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPAKWKGRCEFNF 180
           HTP FLGL+++ GFWK+SNFGKGVIIGVLDGGI P+HPSF D GMP PPAKWKGRC+FN 
Sbjct: 121 HTPRFLGLHQELGFWKESNFGKGVIIGVLDGGIFPSHPSFSDEGMPPPPAKWKGRCDFNA 180

Query: 181 SACNNKLIGARSFNLASKALKGKTTTMDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAV 240
           S CNNKLIGARSFN+A+KA KG   T  + PID DGHGTHTASTAAG FV  AE LGNA 
Sbjct: 181 SDCNNKLIGARSFNIAAKAKKGSAAT--EPPIDVDGHGTHTASTAAGAFVKDAEVLGNAR 240

Query: 241 GTAVGMAPLAHLAIYKVCFGE---DCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFAD 300
           GTAVG+AP AHLAIYKVCFG+   DCP++DILA LDAAV+DGVDVLSLSLG  S P F D
Sbjct: 241 GTAVGIAPHAHLAIYKVCFGDPGDDCPESDILAGLDAAVQDGVDVLSLSLGEDSVPLFND 300

Query: 301 NIAIGALAAIQKGIFVSCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEE 360
            IAIG+ AAIQKGIFVSCSA NSGPFN TLSNEAPWILTV AST+DRR  A A+LGNGE+
Sbjct: 301 TIAIGSFAAIQKGIFVSCSAGNSGPFNGTLSNEAPWILTVGASTVDRRFSATARLGNGEQ 360

Query: 361 FDGESLFQPNDFPQTFLPLVFPGEKNE-TVALCGEGSLKNIDVKGKVVVCERGGGIARIA 420
            DGESL Q ++FP T LPLV+ G   +   +LCGEG+L+ +DVKGK+V+CERGGGI RIA
Sbjct: 361 IDGESLSQHSNFPSTLLPLVYAGMSGKPNSSLCGEGALEGMDVKGKIVLCERGGGIGRIA 420

Query: 421 KGVEVKNAGGAAMILLNLESDGFTTEADAHVLPASHVSHTVALKIKAYINSTTYPIATIV 480
           KG EVKNAGGAAMIL+N E+DGF+T AD HVLPA+HVS    LKIKAYINST  P+ATI+
Sbjct: 421 KGGEVKNAGGAAMILMNEEADGFSTNADVHVLPATHVSFAAGLKIKAYINSTQAPMATIL 480

Query: 481 FKGTIIGDDFSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFN 540
           FKGT+IGD  SP +ASFSSRGPSLASPGILKPDI GPGVSILAAWPFPLDNNTN+KSTFN
Sbjct: 481 FKGTVIGDSSSPFVASFSSRGPSLASPGILKPDIIGPGVSILAAWPFPLDNNTNSKSTFN 540

Query: 541 LVSGTSMSCPHLSGIAALIKSSHPDWSPAVIKSAIMTTANITNLEGNPILDQTLEPADLF 600
           ++SGTSMSCPHLSGIAAL+KSSHP WSPA IKSAIMTTA+  N+EG  I+DQTL+PAD+F
Sbjct: 541 IISGTSMSCPHLSGIAALLKSSHPYWSPAAIKSAIMTTADTLNMEGKLIVDQTLQPADIF 600

Query: 601 AIGAGHVNPSKATDPGLVYDIQPDDYIPYLCGLGYKNNQVSLIAHKPIDCLTTPSIPEGE 660
           A GAGHVNPS+A +PGLVYDIQPDDYIPYLCGLGY +N+VS+I H+ + C   PSIPEGE
Sbjct: 601 ATGAGHVNPSRANNPGLVYDIQPDDYIPYLCGLGYADNEVSIIVHEQVKCSEKPSIPEGE 660

Query: 661 LNYPSFTVELGLPQTFTRTVTNVGDDCEVYTAVIEAPAAVSVTVRPRKLIFSTLNQKATY 720
           LNYPSF V LG  QTFTRTVTNVGD    Y   I +P  V VTV+P KL FS +NQKATY
Sbjct: 661 LNYPSFAVTLGPSQTFTRTVTNVGDVNSAYEVAIVSPPGVDVTVKPSKLYFSKVNQKATY 720

Query: 721 SVTFKRIGSISPSIEFAKGYLKWVSTKHVVGSPIS 742
           SV F R      + E A+GY+ W S K+ V SPI+
Sbjct: 721 SVAFSRTEYGGKTSETAQGYIVWASAKYTVRSPIA 753