Lsi09G003010 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi09G003010
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionGDSL esterase/lipase 5
Locationchr09 : 3098050 .. 3099936 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ACCAAAATCTCTTCCAAGTTCCAACCTTATCCATTTGAACTTTGAATTGCGACTATTTGGAGGCAACGAAAAAGTTTGAGTTGACTAAGAAACTTCAAAGTCAATACATATATAAACTTACAAGTGCCTCTATGTATATATATAAAAACATATGTTGGCAAAACCCCCAATTAAGAGAGAAAAACATGGGAAACTCAAAGTTGGTTACATGTGGATTATTATTGTTGAGCGTTTTGGGAATAATACAAATGTGTGAAGCATTGGGAAAGCAAGATGAAGAAGAAGGTGAAATTCCTCTGTTTGTGTTTGGAGATTCACTTTACGACGTCGGAAATAATAATTACATCAACACAACAACCATTTCTCAGGCCAACTTCCCACCTTACGGCCAAACTTTCTTCAAATTCCCCACCGGAAGATTCTCCGATGGCAGAGTCATTCCTGATTTTATTGGTAACCATCTTCCAAACTTTTTCTAATTTTCAAACATTTATTATTGATTATTGATTATTTATTTATTTTGTTTAATGATTTTGCAGCTGAATATGCAAAGTTGCCATTAATTCTTCCATATCTTTATCCGGGCATTAAGGATTTTGTTAAGGGAGTAAACTTCGCATCTGGAGGAGCTGGAGCTCTCGACCAAACTTTCCCAGGATATGTAACACAATTTTATTTTATTTATTTATTTTTTTTTTTTTTACCGTGTAAGGAAGATTAGGCTACTATAATATGGCCATATTTTTCAGGTGGTGCCGTTGAGAAGACAGGTGGATTATTTTAAAGAAACAGAAACGAGTTTGAGAAAGAAACTTGGAACTTCAGAAACAAAGAAATTACTTTCAAAAGCTGTTTACTTAATCGCCATTGGAAGCAACGACTATGGTGCTTTTGATCCACAATCCAATTTATTTTATCAATCTTACACAACACAACAATATGTTGATTCAGTCATTGGAAACATCACCACTGTCATCAAAGTAATTATAGCACATCGACTATCGAATTGATCTTGCTTTCATTTTGTTCTATATAATACTCTTTGATTTGGGTTTTAATTATATTTAATTACTTGTAGGAAATTTACAAGACAGGAGGAAGGAAGTTTTCAGTGCTAAATATTGGGCCTATCGATCATTTGCCTGCTGTACAAGAAGCTATTATATCCCATGTTAGACCTCTTGCTTGGTTGGAACAATTCAAGCGATTTATTGTTTTACACAACGAACAACTTCCCAAAGCTCTCCAAAAGCTTGCACAAAAGCTTAAAGGATTCGTATATTCTCATACTGATTTCCACACTGCTATCAATGACATAATCCACCACCCAACAAAATACGGTACGTGATTTGAATTATAGAAATTATTCAATGAGACCAGTACAATGTGATGAATAACTTTGAACCGTCGGATGTATGAGAGGTAAACTACAAAAGTATCTCGAATTTGCATCTATGGTTGTCAGAGACATGCATATTTTCGAATCTACATCCAACGGTAATTAGAAGTGATGGTCTCGTTAAATAATTTTTCATTAAACTATACTATTTCTAGTTCCATTTCAATTGGATTATTATGGTTGTAAAGATTTCATCATAAAGTTACTCATGTCGTAATGTGGTTGCTCCTAATACCCACAAATTTATTGAAAAAGGCATGAAAGAAGTGAAGAGTGCATGCTGTGGAAGTGGGGCATTGAGAGGAAGGGATAGCTGTGGAGGGATGAAAGGCATAAAAGAATATGAGTTATGTGAAAATCCAGGGGAGCATGTGTTTTTTGATGCCAACCATGGAACTGATAGAATATACCAGTTTATAGCAGAAATGATGTGGACTGGGACTTCAAATATTACAACTCCTATCAATCTCAATTCCTTATTCTACATA

mRNA sequence

ACCAAAATCTCTTCCAAGTTCCAACCTTATCCATTTGAACTTTGAATTGCGACTATTTGGAGGCAACGAAAAAGTTTGAGTTGACTAAGAAACTTCAAAGTCAATACATATATAAACTTACAAGTGCCTCTATGTATATATATAAAAACATATGTTGGCAAAACCCCCAATTAAGAGAGAAAAACATGGGAAACTCAAAGTTGGTTACATGTGGATTATTATTGTTGAGCGTTTTGGGAATAATACAAATGTGTGAAGCATTGGGAAAGCAAGATGAAGAAGAAGGTGAAATTCCTCTGTTTGTGTTTGGAGATTCACTTTACGACGTCGGAAATAATAATTACATCAACACAACAACCATTTCTCAGGCCAACTTCCCACCTTACGGCCAAACTTTCTTCAAATTCCCCACCGGAAGATTCTCCGATGGCAGAGTCATTCCTGATTTTATTGCTGAATATGCAAAGTTGCCATTAATTCTTCCATATCTTTATCCGGGCATTAAGGATTTTGTTAAGGGAGTAAACTTCGCATCTGGAGGAGCTGGAGCTCTCGACCAAACTTTCCCAGGATATGTGGTGCCGTTGAGAAGACAGGTGGATTATTTTAAAGAAACAGAAACGAGTTTGAGAAAGAAACTTGGAACTTCAGAAACAAAGAAATTACTTTCAAAAGCTGTTTACTTAATCGCCATTGGAAGCAACGACTATGGTGCTTTTGATCCACAATCCAATTTATTTTATCAATCTTACACAACACAACAATATGTTGATTCAGTCATTGGAAACATCACCACTGTCATCAAAGAAATTTACAAGACAGGAGGAAGGAAGTTTTCAGTGCTAAATATTGGGCCTATCGATCATTTGCCTGCTGTACAAGAAGCTATTATATCCCATGTTAGACCTCTTGCTTGGTTGGAACAATTCAAGCGATTTATTGTTTTACACAACGAACAACTTCCCAAAGCTCTCCAAAAGCTTGCACAAAAGCTTAAAGGATTCAGTGCATGCTGTGGAAGTGGGGCATTGAGAGGAAGGGATAGCTGTGGAGGGATGAAAGGCATAAAAGAATATGAGTTATGTGAAAATCCAGGGGAGCATGTGTTTTTTGATGCCAACCATGGAACTGATAGAATATACCAGTTTATAGCAGAAATGATGTGGACTGGGACTTCAAATATTACAACTCCTATCAATCTCAATTCCTTATTCTACATA

Coding sequence (CDS)

ATGTATATATATAAAAACATATGTTGGCAAAACCCCCAATTAAGAGAGAAAAACATGGGAAACTCAAAGTTGGTTACATGTGGATTATTATTGTTGAGCGTTTTGGGAATAATACAAATGTGTGAAGCATTGGGAAAGCAAGATGAAGAAGAAGGTGAAATTCCTCTGTTTGTGTTTGGAGATTCACTTTACGACGTCGGAAATAATAATTACATCAACACAACAACCATTTCTCAGGCCAACTTCCCACCTTACGGCCAAACTTTCTTCAAATTCCCCACCGGAAGATTCTCCGATGGCAGAGTCATTCCTGATTTTATTGCTGAATATGCAAAGTTGCCATTAATTCTTCCATATCTTTATCCGGGCATTAAGGATTTTGTTAAGGGAGTAAACTTCGCATCTGGAGGAGCTGGAGCTCTCGACCAAACTTTCCCAGGATATGTGGTGCCGTTGAGAAGACAGGTGGATTATTTTAAAGAAACAGAAACGAGTTTGAGAAAGAAACTTGGAACTTCAGAAACAAAGAAATTACTTTCAAAAGCTGTTTACTTAATCGCCATTGGAAGCAACGACTATGGTGCTTTTGATCCACAATCCAATTTATTTTATCAATCTTACACAACACAACAATATGTTGATTCAGTCATTGGAAACATCACCACTGTCATCAAAGAAATTTACAAGACAGGAGGAAGGAAGTTTTCAGTGCTAAATATTGGGCCTATCGATCATTTGCCTGCTGTACAAGAAGCTATTATATCCCATGTTAGACCTCTTGCTTGGTTGGAACAATTCAAGCGATTTATTGTTTTACACAACGAACAACTTCCCAAAGCTCTCCAAAAGCTTGCACAAAAGCTTAAAGGATTCAGTGCATGCTGTGGAAGTGGGGCATTGAGAGGAAGGGATAGCTGTGGAGGGATGAAAGGCATAAAAGAATATGAGTTATGTGAAAATCCAGGGGAGCATGTGTTTTTTGATGCCAACCATGGAACTGATAGAATATACCAGTTTATAGCAGAAATGATGTGGACTGGGACTTCAAATATTACAACTCCTATCAATCTCAATTCCTTATTCTACATA

Protein sequence

MYIYKNICWQNPQLREKNMGNSKLVTCGLLLLSVLGIIQMCEALGKQDEEEGEIPLFVFGDSLYDVGNNNYINTTTISQANFPPYGQTFFKFPTGRFSDGRVIPDFIAEYAKLPLILPYLYPGIKDFVKGVNFASGGAGALDQTFPGYVVPLRRQVDYFKETETSLRKKLGTSETKKLLSKAVYLIAIGSNDYGAFDPQSNLFYQSYTTQQYVDSVIGNITTVIKEIYKTGGRKFSVLNIGPIDHLPAVQEAIISHVRPLAWLEQFKRFIVLHNEQLPKALQKLAQKLKGFSACCGSGALRGRDSCGGMKGIKEYELCENPGEHVFFDANHGTDRIYQFIAEMMWTGTSNITTPINLNSLFYI
Homology
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BLAST of Lsi09G003010 vs. Swiss-Prot
Match: GLIP1_ARATH (GDSL esterase/lipase 1 OS=Arabidopsis thaliana GN=GLIP1 PE=1 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 1.2e-82
Identity = 172/374 (45.99%), Postives = 229/374 (61.23%), Query Frame = 1 

Query: 19  MGNSKLVTCGLLLLSVLGIIQMCEALGKQDEEEGEIPLFVFGDSLYDVGNNNYINTTTIS 78
           M NS+LV+   L  +++  I     +   +    +  LFVFGDS++D GNNNYI+T +  
Sbjct: 1   MENSQLVSITFLAYTIIISIGSINCIDNNNLVTNQSALFVFGDSVFDAGNNNYIDTLSSV 60

Query: 79  QANFPPYGQTFFKFPTGRFSDGRVIPDFIAEYAKLPLILPYLYP--GIKDFVKGVNFASG 138
           ++N+ PYGQT FK PTGR SDGR+IPDFIAEYA LPLI P L P  G   F  GVNFASG
Sbjct: 61  RSNYWPYGQTTFKSPTGRVSDGRLIPDFIAEYAWLPLIPPNLQPFNGNSQFAYGVNFASG 120

Query: 139 GAGALDQTFPGYVVPLRRQVDYFKETETSLRKKLGTSETKKLLSKAVYLIAIGSNDYG-A 198
           GAGAL  TF G V+ LR Q++ FK+ E  LR KLG +E K+++S+AVYL  IG NDY   
Sbjct: 121 GAGALVGTFSGLVINLRTQLNNFKKVEEMLRSKLGDAEGKRVISRAVYLFHIGLNDYQYP 180

Query: 199 FDPQSNLFYQSYTTQQYVDSVIGNITTVIKEIYKTGGRKFSVLNIGPIDHLPAVQEAIIS 258
           F   S+LF QS + ++YVD V+GN+T V KE+Y  GGRKF +LN GP D  PA    +I 
Sbjct: 181 FTTNSSLF-QSISNEKYVDYVVGNMTDVFKEVYNLGGRKFGILNTGPYDCAPA--SLVID 240

Query: 259 HVRPLAWLEQFKRFIVLHNEQLPKALQKLAQKLKGF------------------------ 318
             +  +  +     I +HNE+L   L++L  +L GF                        
Sbjct: 241 QTKIRSCFQPVTELINMHNEKLLNGLRRLNHELSGFKYALHDYHTSLSERMNDPSKYGFK 300

Query: 319 ---SACCGSGALRGRDSCGGMKGIKE-YELCENPGEHVFFDANHGTDRIYQFIAEMMWTG 362
               ACCGSG LRG ++CGG  G+ + YELCEN  +++FFD  H T++  + IAE++W+G
Sbjct: 301 EGKKACCGSGPLRGINTCGGRMGLSQSYELCENVTDYLFFDPFHLTEKANRQIAELIWSG 360

[Close]
BLAST of Lsi09G003010 vs. Swiss-Prot
Match: GLIP2_ARATH (GDSL esterase/lipase 2 OS=Arabidopsis thaliana GN=GLIP2 PE=2 SV=1)

HSP 1 Score: 291.6 bits (745), Expect = 1.2e-77
Identity = 162/337 (48.07%), Postives = 209/337 (62.02%), Query Frame = 1 

Query: 56  LFVFGDSLYDVGNNNYINTTTISQANFPPYGQTFFKFPTGRFSDGRVIPDFIAEYAKLPL 115
           LFVFGDS++D GNNNYI+T    ++N+ PYGQT FKFPTGR SDGR IPDFIAEYA LPL
Sbjct: 40  LFVFGDSVFDAGNNNYIDTLPSFRSNYWPYGQTTFKFPTGRVSDGRTIPDFIAEYAWLPL 99

Query: 116 ILPYLYP--GIKDFVKGVNFASGGAGALDQTFPGYVVPLRRQVDYFKETETSLRKKLGTS 175
           I  YL P  G   F  GV+FAS GAGAL  TFPG V+ L+ Q++ FK+ E  LR  LG +
Sbjct: 100 IPAYLQPSNGKNQFPYGVSFASAGAGALVGTFPGMVINLKSQLNNFKKVEKLLRSTLGEA 159

Query: 176 ETKKLLSKAVYLIAIGSNDYG-AFDPQSNLFYQSYTTQQYVDSVIGNITTVIKEIYKTGG 235
           + K ++S+AVYL  IG NDY   F   S++F QS   + YVD V+GN T VIKE+YK GG
Sbjct: 160 QGKMVISRAVYLFHIGVNDYQYPFSTNSSIF-QSSPQEIYVDFVVGNTTAVIKEVYKIGG 219

Query: 236 RKFSVLNIGPIDHLPAVQEAIISHVRPLAWLEQFKRFIVLHNEQLPKALQKLAQKLKGFS 295
           RKF  LN+G  D  PA    II   +     +     I LHNE+L   L++L ++L GF 
Sbjct: 220 RKFGFLNMGAYDCAPA--SLIIDQTKIGTCFKPVTELINLHNEKLESGLRRLERELSGFK 279

Query: 296 ---------------------------ACCGSGALRGRDSCGGMKGIKE-YELCENPGEH 355
                                      ACCG+G LRG ++CGG  G+ + YELCE   ++
Sbjct: 280 YALHDYHTSLSVRMNNPSKYGFKEGKMACCGTGPLRGINTCGGRMGVSQSYELCEKVTDY 339

Query: 356 VFFDANHGTDRIYQFIAEMMWTGTSNITTPINLNSLF 362
           +FFD  H T++ +Q IAE++W+G +N+T P NL +LF
Sbjct: 340 LFFDHFHLTEKAHQQIAELIWSGPTNVTKPYNLQALF 373

[Close]
BLAST of Lsi09G003010 vs. Swiss-Prot
Match: GLIP5_ARATH (GDSL esterase/lipase 5 OS=Arabidopsis thaliana GN=GLIP5 PE=2 SV=2)

HSP 1 Score: 291.2 bits (744), Expect = 1.6e-77
Identity = 161/341 (47.21%), Postives = 208/341 (61.00%), Query Frame = 1 

Query: 56  LFVFGDSLYDVGNNNYINTTTISQANFPPYGQTFFKFPTGRFSDGRVIPDFIAEYAKLPL 115
           LF+FGDS  D GNNNYINTTT+ QANFPPYGQTFF  PTGRFSDGR+I DFIAEYA LPL
Sbjct: 49  LFLFGDSFLDAGNNNYINTTTLDQANFPPYGQTFFGLPTGRFSDGRLISDFIAEYANLPL 108

Query: 116 ILPYLYPG-IKDFVKGVNFASGGAGALDQTFPGYVVPLRRQVDYFKETETSLRKKLGTSE 175
           I P+L PG  +  + GVNFAS GAGAL +TF G V+ LR Q+D++K+ E   R   G  E
Sbjct: 109 IPPFLEPGNSQKKLYGVNFASAGAGALVETFQGSVINLRTQLDHYKKVERLWRTNFGKEE 168

Query: 176 TKKLLSKAVYLIAIGSNDYGA-FDPQSNLFYQSYTTQQYVDSVIGNITTVIKEIYKTGGR 235
           +KK +S+AVYLI+IGSNDY + F    +L     +  Q+VD VIGN+TT I EIYK GGR
Sbjct: 169 SKKRISRAVYLISIGSNDYSSIFLTNQSL---PISMSQHVDIVIGNLTTFIHEIYKIGGR 228

Query: 236 KFSVLNIGPIDHLPAVQEAIISHVRPLAWLEQFKRFIVLHNEQLPKALQKLAQKLKGF-- 295
           KF  LN+  +   PA++  I+      + L    R   +HN  L   L ++ +++KGF  
Sbjct: 229 KFGFLNVPDLGCFPALR--ILQPKNDDSCLRDASRLASMHNRALTNLLFQMQRQVKGFKF 288

Query: 296 -------------------------SACCGSGALRGRDSCGGMKGIKEYELCENPGEHVF 355
                                     ACCG+G  RG  SCGG + +KEY+LCENP +++F
Sbjct: 289 SLFDMNKSLRLRMQHPSKFGFKEGEEACCGTGKWRGVFSCGGKRIVKEYQLCENPKDYIF 348

Query: 356 FDANHGTDRIYQFIAEMMWTG----TSNITTPINLNSLFYI 364
           +D+ H T   Y   A ++W G     S +  P N+N+LF I
Sbjct: 349 WDSLHLTQNTYNQFANLIWNGGHMSDSLVVGPYNINNLFQI 384

[Close]
BLAST of Lsi09G003010 vs. Swiss-Prot
Match: GRIP4_ARATH (GDSL esterase/lipase 4 OS=Arabidopsis thaliana GN=GLIP4 PE=2 SV=2)

HSP 1 Score: 275.0 bits (702), Expect = 1.2e-72
Identity = 149/350 (42.57%), Postives = 210/350 (60.00%), Query Frame = 1 

Query: 46  KQDEEEGEIPLFVFGDSLYDVGNNNYINTTTISQANFPPYGQTFFKFPTGRFSDGRVIPD 105
           K+D +  +  LF FGDSL++ GNNNY ++ +  ++NF PYG+T FKFPTGR SDGR++ D
Sbjct: 28  KEDLKTNQAALFAFGDSLFEAGNNNYFDSISSFRSNFWPYGKTTFKFPTGRVSDGRIMID 87

Query: 106 FIAEYAKLPLILPYLYPGIKD--FVKGVNFASGGAGALDQTFPGYVVPLRR----QVDYF 165
           FIAEYA LPLI P L PG  +     G+NFA+  AG    TFPG V  L +    Q++ F
Sbjct: 88  FIAEYAWLPLIPPNLQPGYSNSQLTYGLNFATTAAGVFAGTFPGSVTNLSKDLGTQLNNF 147

Query: 166 KETETSLRKKLGTSETKKLLSKAVYLIAIGSNDYGAFDPQSNLFYQSYTTQQYVDSVIGN 225
           K  E +LR  LG +E ++++SKAVYL  IG+NDY      +   + + T ++++D VIGN
Sbjct: 148 KNVEKTLRSNLGDAEARRVISKAVYLFHIGANDYQYPFFANTSTFSNTTKERFIDFVIGN 207

Query: 226 ITTVIKEIYKTGGRKFSVLNIGPIDHLPAVQEAIISHVRPLAWLEQFKRFIVLHNEQLPK 285
            TTVI+E+YK G RKF  L++GP    P+    II+  +  +  E     I LHN++ PK
Sbjct: 208 TTTVIEELYKLGARKFGFLSLGPFGCTPSA--LIINSTKIGSCFEPVTELINLHNQEFPK 267

Query: 286 ALQKLAQKLKGFS---------------------------ACCGSGALRGRDSCGGMKGI 345
            L++L ++L GF                            ACCGSG LRG ++CG   G 
Sbjct: 268 VLRRLERRLSGFKYALHDFHTSLSQRINNPSRYGFKEGEMACCGSGPLRGINTCGFRNGP 327

Query: 346 KE-YELCENPGEHVFFDANHGTDRIYQFIAEMMWTGTSNITTPINLNSLF 362
            + Y+LCEN  ++VFFD +H T+  +Q IAE++W+G  N+T P NL +LF
Sbjct: 328 SQGYKLCENADDYVFFDPSHLTETAHQQIAELIWSGPPNVTAPYNLKTLF 375

[Close]
BLAST of Lsi09G003010 vs. Swiss-Prot
Match: GLIP3_ARATH (GDSL esterase/lipase 3 OS=Arabidopsis thaliana GN=GLIP3 PE=2 SV=2)

HSP 1 Score: 260.4 bits (664), Expect = 3.0e-68
Identity = 146/336 (43.45%), Postives = 202/336 (60.12%), Query Frame = 1 

Query: 56  LFVFGDSLYDVGNNNYINTTTISQANFPPYGQTFFKFPTGRFSDGRVIPDFIAEYAKLPL 115
           LFVFGDSL+D GNNNYINT +  ++N  PYGQT FKFPTGR SDG        E A LP 
Sbjct: 36  LFVFGDSLFDAGNNNYINTVSSFRSNIWPYGQTNFKFPTGRLSDG-------PEKAWLPS 95

Query: 116 ILPYLYP--GIKDFVKGVNFASGGAGALDQTFPGYVVPLRRQVDYFKETETSLRKKLGTS 175
           I P L P  G   F  GV+FAS GAGAL ++F G V+ L  Q++ FK+ E SLR +LG +
Sbjct: 96  IPPNLQPNNGNNQFTYGVSFASAGAGALAESFLGMVINLGTQLNNFKDVEKSLRSELGDA 155

Query: 176 ETKKLLSKAVYLIAIGSNDYGAFDPQSNLFYQSYTTQQYVDSVIGNITTVIKEIYKTGGR 235
           ETK++ S+AVYL  IG+NDY      ++  ++S + +++VD VIGNIT VI+E+YK GGR
Sbjct: 156 ETKRVFSRAVYLFHIGANDYFYPFSANSSTFKSNSKEKFVDFVIGNITFVIEEVYKMGGR 215

Query: 236 KFSVLNIGPIDHLPAVQEAIISHVRPLAWLEQFKRFIVLHNEQLPKALQKLAQKLKGF-- 295
           KF  LN+GP +  P     I    +  +  +     I +HN++ P  L++L ++L GF  
Sbjct: 216 KFGFLNVGPYECSP--NSLIRDRTKIGSCFKPVAELIDMHNKKFPDVLRRLQRQLSGFRY 275

Query: 296 -------------------------SACCGSGALRGRDSCGGMKGIKE-YELCENPGEHV 355
                                     ACCGSG LRG ++CG   G  + Y LCEN  +++
Sbjct: 276 ALHDYHTSLSERINSPSKYGFKEGKKACCGSGPLRGINTCGNRIGPSQGYGLCENVTDYL 335

Query: 356 FFDANHGTDRIYQFIAEMMWTGTSNITTPINLNSLF 362
           F+D++H T++ ++ IAE++W G  N+T P NL +LF
Sbjct: 336 FYDSSHLTEKAHRQIAELIWNGPPNVTRPYNLKALF 362

[Close]
BLAST of Lsi09G003010 vs. TrEMBL
Match: A0A0A0KP02_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G155530 PE=4 SV=1)

HSP 1 Score: 442.6 bits (1137), Expect = 4.7e-121
Identity = 221/284 (77.82%), Postives = 235/284 (82.75%), Query Frame = 1 

Query: 107 IAEYAKLPLILPYLYPGIKDFVKGVNFASGGAGALDQTFPGYVVPLRRQVDYFKETETSL 166
           I+EYAKLPLILPYLYPGIKDFVKGVNFASGGAG LD TFPGYVV LRRQV+YFKE E SL
Sbjct: 21  ISEYAKLPLILPYLYPGIKDFVKGVNFASGGAGVLDTTFPGYVVTLRRQVNYFKEMERSL 80

Query: 167 RKKLGTSETKKLLSKAVYLIAIGSNDYGAFDPQSNLFYQSYTTQQYVDSVIGNITTVIKE 226
           RKKLGTS+TKKLLSKAVYLIAIGS DY AFDP+SN  YQSYTTQQYVD VIGN+T+ I+E
Sbjct: 81  RKKLGTSKTKKLLSKAVYLIAIGSGDYDAFDPKSNSLYQSYTTQQYVDLVIGNMTSFIEE 140

Query: 227 IYKTGGRKFSVLNIGPIDHLPAVQEAIISHVRPLAWLEQFKRFIVLHNEQLPKALQKLAQ 286
           IYKTGGRKFSVLNIGPIDHLPAVQEAIISH R  AW+EQFK+FI LHNE+LPKALQ LAQ
Sbjct: 141 IYKTGGRKFSVLNIGPIDHLPAVQEAIISHYRTPAWMEQFKQFIGLHNEKLPKALQNLAQ 200

Query: 287 KLKGF---------------------------SACCGSGALRGRDSCGGMKGIKEYELCE 346
           K KG                            S CCGSGA RG+ SCGGM+GIKEYELCE
Sbjct: 201 KFKGLLYSHTDFHTAISNIIHHPTKYGMKEVKSGCCGSGAFRGKSSCGGMRGIKEYELCE 260

Query: 347 NPGEHVFFDANHGTDRIYQFIAEMMWTGTSNITTPINLNSLFYI 364
           NP EHVFFDANHGTDRIY+F+AEMMWTGTSNITTPINLNSLFYI
Sbjct: 261 NPEEHVFFDANHGTDRIYKFVAEMMWTGTSNITTPINLNSLFYI 304

[Close]
BLAST of Lsi09G003010 vs. TrEMBL
Match: D7TZL6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_09s0002g00540 PE=4 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 9.9e-95
Identity = 180/340 (52.94%), Postives = 225/340 (66.18%), Query Frame = 1 

Query: 49  EEEGEIPLFVFGDSLYDVGNNNYINTTTISQANFPPYGQTFFKFPTGRFSDGRVIPDFIA 108
           + +  +  F+FGDSL D GNNNYINTTT  QANF PYG+TFFK+PTGRFSDGR+IPDFIA
Sbjct: 33  QPQNHVAFFIFGDSLLDPGNNNYINTTTEDQANFRPYGETFFKYPTGRFSDGRLIPDFIA 92

Query: 109 EYAKLPLILPYLYPGIKDFVKGVNFASGGAGALDQTFPGYVVPLRRQVDYFKETETSLRK 168
           EYAKLPLI PYL PG   F  G NFASGGAGALD+   G VV L  Q+ YFK+ E  LR+
Sbjct: 93  EYAKLPLIPPYLQPGNHQFTYGANFASGGAGALDEINQGLVVNLNTQLRYFKKVEKHLRE 152

Query: 169 KLGTSETKKLLSKAVYLIAIGSNDYGAFDPQSNLFYQSYTTQQYVDSVIGNITTVIKEIY 228
           KLG  E+KKLL +AVYLI+IG NDY +   ++   +Q Y+ +QY+D V+GN+T VI+EIY
Sbjct: 153 KLGDEESKKLLLEAVYLISIGGNDYISPLFRNYSVFQIYSHRQYLDMVMGNLTVVIQEIY 212

Query: 229 KTGGRKFSVLNIGPIDHLPAVQEAIISHVRPLAWLEQFKRFIVLHNEQLPKALQKLAQKL 288
           + GGRKF  +N+GP+  LPA++   +        +E+    + LHN  LP+ LQKL  KL
Sbjct: 213 QKGGRKFGFVNMGPLGCLPAMKAIKLQQGGAGECMEEATVLVKLHNRVLPEVLQKLGSKL 272

Query: 289 KGFS---------------------------ACCGSGALRGRDSCGGMKGIKEYELCENP 348
           KGF                            ACCGSG  RG  SCGGM+G KEYELC N 
Sbjct: 273 KGFKYSIFDFYTTAKERMDNPSKYGFKEAKIACCGSGPYRGLYSCGGMRGTKEYELCSNV 332

Query: 349 GEHVFFDANHGTDRIYQFIAEMMWTGTSNITTPINLNSLF 362
            E++FFD+ H TDR+YQ +AE++W+GT N+  P NL  LF
Sbjct: 333 SEYMFFDSFHPTDRVYQQLAELVWSGTHNVIKPYNLKQLF 372

[Close]
BLAST of Lsi09G003010 vs. TrEMBL
Match: D7TZL3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_09s0002g00510 PE=4 SV=1)

HSP 1 Score: 343.2 bits (879), Expect = 3.9e-91
Identity = 181/340 (53.24%), Postives = 218/340 (64.12%), Query Frame = 1 

Query: 49  EEEGEIPLFVFGDSLYDVGNNNYINTTTISQANFPPYGQTFFKFPTGRFSDGRVIPDFIA 108
           + E    LF+FGDS++D GNN YINTTT  Q NF PYG+TFF +PTGR SDGR+IPDFIA
Sbjct: 30  QPEKHAALFIFGDSIFDAGNNIYINTTTDYQRNFWPYGETFFDYPTGRASDGRLIPDFIA 89

Query: 109 EYAKLPLILPYLYPGIKDFVKGVNFASGGAGALDQTFPGYVVPLRRQVDYFKETETSLRK 168
           EYAKLP + PYL PG   F  G NFASGGAGALDQT  G VV L  Q+ YFK+ E  LR+
Sbjct: 90  EYAKLPFLPPYLQPGNNQFTYGSNFASGGAGALDQTNQGLVVNLNTQLTYFKDVEKLLRQ 149

Query: 169 KLGTSETKKLLSKAVYLIAIGSNDYGAFDPQSNLFYQSYTTQQYVDSVIGNITTVIKEIY 228
           KLG    KK+L +AVYLI IGSNDY +    ++   QSY+ +QYV  VIGN+T VIKEIY
Sbjct: 150 KLGDEAAKKMLFEAVYLINIGSNDYLSPFLWNSTVLQSYSHEQYVHMVIGNLTVVIKEIY 209

Query: 229 KTGGRKFSVLNIGPIDHLPAVQEAIISHVRPLAWLEQFKRFIVLHNEQLPKALQKLAQKL 288
           K GGRKF +L++GP+  +P ++E  +     +  +E+      LHN  L K LQ+L  KL
Sbjct: 210 KKGGRKFGLLDVGPLGCVPIMKEIKLQQ-GGMGCIEESTELAKLHNIALSKVLQELESKL 269

Query: 289 KGFS---------------------------ACCGSGALRGRDSCGGMKGIKEYELCENP 348
           KGF                            ACCGSG  RG  SCGG   IKEYELC N 
Sbjct: 270 KGFKYSISNFYTFLEERMNNPSKYGFKEGKIACCGSGPFRGLSSCGGKSSIKEYELCSNV 329

Query: 349 GEHVFFDANHGTDRIYQFIAEMMWTGTSNITTPINLNSLF 362
            E+VFFD+ H TDR YQ IAE++W+GT NIT P NL +LF
Sbjct: 330 SEYVFFDSVHPTDRAYQQIAELIWSGTRNITGPYNLKALF 368

[Close]
BLAST of Lsi09G003010 vs. TrEMBL
Match: A0A061E3A2_THECC (GDSL lipase 1, putative OS=Theobroma cacao GN=TCM_007501 PE=4 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 7.3e-90
Identity = 177/370 (47.84%), Postives = 234/370 (63.24%), Query Frame = 1 

Query: 19  MGNSKLVTCGLLLLSVLGIIQMCEALGKQDEEEGEIPLFVFGDSLYDVGNNNYINTTTIS 78
           M +S++  C LL  + L  +  C  L      E  + LF+FGDSL+D GNNNYINTT   
Sbjct: 1   MASSRIHICFLLYFATLLNLSCCYIL-----PENHVALFIFGDSLFDAGNNNYINTTVNY 60

Query: 79  QANFPPYGQTFFKFPTGRFSDGRVIPDFIAEYAKLPLILPYLYPGIKDFVKGVNFASGGA 138
           QANF PYG+TFFK+PTGRFSDGR++PDFIAEYA LPLI PY  P    FV GVNFAS GA
Sbjct: 61  QANFWPYGETFFKYPTGRFSDGRLLPDFIAEYAGLPLIPPYFQPVNNKFVDGVNFASAGA 120

Query: 139 GALDQTFPGYVVPLRRQVDYFKETETSLRKKLGTSETKKLLSKAVYLIAIGSNDYGAFDP 198
           GAL +T  G+V+ L  QV YFK+ E  LR++LG +E K+LL +A+Y+I+IGSNDY     
Sbjct: 121 GALVETHQGFVINLNTQVSYFKDVEKLLRQELGDAEAKRLLGRALYIISIGSNDYFVRIT 180

Query: 199 QSNLFYQSYTTQQYVDSVIGNITTVIKEIYKTGGRKFSVLNIGPIDHLPAVQEAIISHVR 258
           Q++   QSY+ ++YV  VIGN+T  IKEI+K GGRKF  L++GP+  +P ++  +     
Sbjct: 181 QNSSVLQSYSEEEYVAIVIGNLTVAIKEIHKKGGRKFGFLSLGPLGCIPGMKVLVSGSTG 240

Query: 259 PLAWLEQFKRFIVLHNEQLPKALQKLAQKLKGFS-------------------------- 318
             + +++      LHN+ L  ALQKL  +L+GF                           
Sbjct: 241 --SCVDKATTLAHLHNKALSIALQKLENRLEGFKFANHDLYTSVSERMNNPSKYGFKVGN 300

Query: 319 -ACCGSGALRGRDSCGGMKGIKEYELCENPGEHVFFDANHGTDRIYQFIAEMMWTGTSNI 362
            ACCGSG  RG+ SCGG + IKEY+LCE   E++FFD+ H T+  ++ IAE++W GT NI
Sbjct: 301 MACCGSGPYRGQSSCGGKRQIKEYQLCEKASEYLFFDSGHPTEMAHRQIAELIWHGTPNI 360

[Close]
BLAST of Lsi09G003010 vs. TrEMBL
Match: A0A067L971_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_24643 PE=4 SV=1)

HSP 1 Score: 330.9 bits (847), Expect = 2.0e-87
Identity = 177/370 (47.84%), Postives = 232/370 (62.70%), Query Frame = 1 

Query: 19  MGNSKLVTCGLLLLSVLGIIQMCEALGKQDEEEGEIPLFVFGDSLYDVGNNNYINTTTIS 78
           M NSKL    L+  S L I      L  QD +   + LF+FGDSL+D GNNNY+ T  I+
Sbjct: 1   MANSKLGFSFLIFCSTLLISTKSNNLPSQDHQR--VALFIFGDSLFDAGNNNYLKTD-IA 60

Query: 79  QANFPPYGQTFFKFPTGRFSDGRVIPDFIAEYAKLPLILPYLYPGIKDFVKGVNFASGGA 138
           +ANF PYG+TFFK PTGR+SDGR+IPDFIAEY KLP I PYL P    + KGVNFASGGA
Sbjct: 61  KANFWPYGETFFKHPTGRYSDGRIIPDFIAEYLKLPFIRPYLEPNNHHYTKGVNFASGGA 120

Query: 139 GALDQTFPGYVVPLRRQVDYFKETETSLRKKLGTSETKKLLSKAVYLIAIGSNDY-GAFD 198
           GAL +TFPG V+ L+ QV YFK+ +  LR++LG SET  LLSKA+YL +IG+NDY  +  
Sbjct: 121 GALSETFPGQVLDLKTQVTYFKKVKKQLRQELGDSETNALLSKAIYLFSIGTNDYFSSLL 180

Query: 199 PQSNLFYQSYTTQQYVDSVIGNITTVIKEIYKTGGRKFSVLNIGPIDHLPAVQEAIISHV 258
             S+      + ++YV  VIGN+TT IKEIY+ GGRKF VL+IG ++ LP V+   I  +
Sbjct: 181 TNSSTVVNDNSRKEYVAMVIGNLTTAIKEIYRNGGRKFGVLSIGSLEFLPVVRA--IEQI 240

Query: 259 RPLAWLEQFKRFIVLHNEQLPKALQKLAQKLKGF-------------------------- 318
               + EQ + F  LHN  LP  L+KL ++L+GF                          
Sbjct: 241 NRGGFREQLRTFAKLHNNALPHVLKKLQRQLQGFKYSYFDTYTSSIYRINNPSKYGFKVA 300

Query: 319 -SACCGSGALRGRDSCGGMKGIKEYELCENPGEHVFFDANHGTDRIYQFIAEMMWTGTSN 361
            SACCG+G  RG ++CGG     E+ELC+NP E++FFD  H T++    +A++MW+G   
Sbjct: 301 KSACCGNGPYRGIENCGGT----EFELCKNPSEYLFFDGGHPTEKFNYQLAKLMWSGNFR 360

[Close]
BLAST of Lsi09G003010 vs. TAIR10
Match: AT5G40990.1 (AT5G40990.1 GDSL lipase 1)

HSP 1 Score: 308.1 bits (788), Expect = 7.0e-84
Identity = 172/374 (45.99%), Postives = 229/374 (61.23%), Query Frame = 1 

Query: 19  MGNSKLVTCGLLLLSVLGIIQMCEALGKQDEEEGEIPLFVFGDSLYDVGNNNYINTTTIS 78
           M NS+LV+   L  +++  I     +   +    +  LFVFGDS++D GNNNYI+T +  
Sbjct: 1   MENSQLVSITFLAYTIIISIGSINCIDNNNLVTNQSALFVFGDSVFDAGNNNYIDTLSSV 60

Query: 79  QANFPPYGQTFFKFPTGRFSDGRVIPDFIAEYAKLPLILPYLYP--GIKDFVKGVNFASG 138
           ++N+ PYGQT FK PTGR SDGR+IPDFIAEYA LPLI P L P  G   F  GVNFASG
Sbjct: 61  RSNYWPYGQTTFKSPTGRVSDGRLIPDFIAEYAWLPLIPPNLQPFNGNSQFAYGVNFASG 120

Query: 139 GAGALDQTFPGYVVPLRRQVDYFKETETSLRKKLGTSETKKLLSKAVYLIAIGSNDYG-A 198
           GAGAL  TF G V+ LR Q++ FK+ E  LR KLG +E K+++S+AVYL  IG NDY   
Sbjct: 121 GAGALVGTFSGLVINLRTQLNNFKKVEEMLRSKLGDAEGKRVISRAVYLFHIGLNDYQYP 180

Query: 199 FDPQSNLFYQSYTTQQYVDSVIGNITTVIKEIYKTGGRKFSVLNIGPIDHLPAVQEAIIS 258
           F   S+LF QS + ++YVD V+GN+T V KE+Y  GGRKF +LN GP D  PA    +I 
Sbjct: 181 FTTNSSLF-QSISNEKYVDYVVGNMTDVFKEVYNLGGRKFGILNTGPYDCAPA--SLVID 240

Query: 259 HVRPLAWLEQFKRFIVLHNEQLPKALQKLAQKLKGF------------------------ 318
             +  +  +     I +HNE+L   L++L  +L GF                        
Sbjct: 241 QTKIRSCFQPVTELINMHNEKLLNGLRRLNHELSGFKYALHDYHTSLSERMNDPSKYGFK 300

Query: 319 ---SACCGSGALRGRDSCGGMKGIKE-YELCENPGEHVFFDANHGTDRIYQFIAEMMWTG 362
               ACCGSG LRG ++CGG  G+ + YELCEN  +++FFD  H T++  + IAE++W+G
Sbjct: 301 EGKKACCGSGPLRGINTCGGRMGLSQSYELCENVTDYLFFDPFHLTEKANRQIAELIWSG 360

[Close]
BLAST of Lsi09G003010 vs. TAIR10
Match: AT1G53920.1 (AT1G53920.1 GDSL-motif lipase 5)

HSP 1 Score: 291.2 bits (744), Expect = 8.8e-79
Identity = 161/341 (47.21%), Postives = 208/341 (61.00%), Query Frame = 1 

Query: 56  LFVFGDSLYDVGNNNYINTTTISQANFPPYGQTFFKFPTGRFSDGRVIPDFIAEYAKLPL 115
           LF+FGDS  D GNNNYINTTT+ QANFPPYGQTFF  PTGRFSDGR+I DFIAEYA LPL
Sbjct: 49  LFLFGDSFLDAGNNNYINTTTLDQANFPPYGQTFFGLPTGRFSDGRLISDFIAEYANLPL 108

Query: 116 ILPYLYPG-IKDFVKGVNFASGGAGALDQTFPGYVVPLRRQVDYFKETETSLRKKLGTSE 175
           I P+L PG  +  + GVNFAS GAGAL +TF G V+ LR Q+D++K+ E   R   G  E
Sbjct: 109 IPPFLEPGNSQKKLYGVNFASAGAGALVETFQGSVINLRTQLDHYKKVERLWRTNFGKEE 168

Query: 176 TKKLLSKAVYLIAIGSNDYGA-FDPQSNLFYQSYTTQQYVDSVIGNITTVIKEIYKTGGR 235
           +KK +S+AVYLI+IGSNDY + F    +L     +  Q+VD VIGN+TT I EIYK GGR
Sbjct: 169 SKKRISRAVYLISIGSNDYSSIFLTNQSL---PISMSQHVDIVIGNLTTFIHEIYKIGGR 228

Query: 236 KFSVLNIGPIDHLPAVQEAIISHVRPLAWLEQFKRFIVLHNEQLPKALQKLAQKLKGF-- 295
           KF  LN+  +   PA++  I+      + L    R   +HN  L   L ++ +++KGF  
Sbjct: 229 KFGFLNVPDLGCFPALR--ILQPKNDDSCLRDASRLASMHNRALTNLLFQMQRQVKGFKF 288

Query: 296 -------------------------SACCGSGALRGRDSCGGMKGIKEYELCENPGEHVF 355
                                     ACCG+G  RG  SCGG + +KEY+LCENP +++F
Sbjct: 289 SLFDMNKSLRLRMQHPSKFGFKEGEEACCGTGKWRGVFSCGGKRIVKEYQLCENPKDYIF 348

Query: 356 FDANHGTDRIYQFIAEMMWTG----TSNITTPINLNSLFYI 364
           +D+ H T   Y   A ++W G     S +  P N+N+LF I
Sbjct: 349 WDSLHLTQNTYNQFANLIWNGGHMSDSLVVGPYNINNLFQI 384

[Close]
BLAST of Lsi09G003010 vs. TAIR10
Match: AT1G53940.1 (AT1G53940.1 GDSL-motif lipase 2)

HSP 1 Score: 279.3 bits (713), Expect = 3.5e-75
Identity = 156/326 (47.85%), Postives = 201/326 (61.66%), Query Frame = 1 

Query: 56  LFVFGDSLYDVGNNNYINTTTISQANFPPYGQTFFKFPTGRFSDGRVIPDFIAEYAKLPL 115
           LFVFGDS++D GNNNYI+T    ++N+ PYGQT FKFPTGR SDGR IPDFIAEYA LPL
Sbjct: 40  LFVFGDSVFDAGNNNYIDTLPSFRSNYWPYGQTTFKFPTGRVSDGRTIPDFIAEYAWLPL 99

Query: 116 ILPYLYP--GIKDFVKGVNFASGGAGALDQTFPGYVVPLRRQVDYFKETETSLRKKLGTS 175
           I  YL P  G   F  GV+FAS GAGAL  TFPG V+ L+ Q++ FK+ E  LR  LG +
Sbjct: 100 IPAYLQPSNGKNQFPYGVSFASAGAGALVGTFPGMVINLKSQLNNFKKVEKLLRSTLGEA 159

Query: 176 ETKKLLSKAVYLIAIGSNDYG-AFDPQSNLFYQSYTTQQYVDSVIGNITTVIKEIYKTGG 235
           + K ++S+AVYL  IG NDY   F   S++F QS   + YVD V+GN T VIKE+YK GG
Sbjct: 160 QGKMVISRAVYLFHIGVNDYQYPFSTNSSIF-QSSPQEIYVDFVVGNTTAVIKEVYKIGG 219

Query: 236 RKFSVLNIGPIDHLPAVQEAIISHVRPLAWLEQFKRFIVLHNEQLPKALQKLAQKLKGFS 295
           RKF  LN+G  D  PA    II   +     +     I LHNE+L   L++L ++L GF 
Sbjct: 220 RKFGFLNMGAYDCAPA--SLIIDQTKIGTCFKPVTELINLHNEKLESGLRRLERELSGFK 279

Query: 296 ---------------------------ACCGSGALRGRDSCGGMKGIKE-YELCENPGEH 351
                                      ACCG+G LRG ++CGG  G+ + YELCE   ++
Sbjct: 280 YALHDYHTSLSVRMNNPSKYGFKEGKMACCGTGPLRGINTCGGRMGVSQSYELCEKVTDY 339

[Close]
BLAST of Lsi09G003010 vs. TAIR10
Match: AT3G14225.1 (AT3G14225.1 GDSL-motif lipase 4)

HSP 1 Score: 275.0 bits (702), Expect = 6.6e-74
Identity = 149/350 (42.57%), Postives = 210/350 (60.00%), Query Frame = 1 

Query: 46  KQDEEEGEIPLFVFGDSLYDVGNNNYINTTTISQANFPPYGQTFFKFPTGRFSDGRVIPD 105
           K+D +  +  LF FGDSL++ GNNNY ++ +  ++NF PYG+T FKFPTGR SDGR++ D
Sbjct: 28  KEDLKTNQAALFAFGDSLFEAGNNNYFDSISSFRSNFWPYGKTTFKFPTGRVSDGRIMID 87

Query: 106 FIAEYAKLPLILPYLYPGIKD--FVKGVNFASGGAGALDQTFPGYVVPLRR----QVDYF 165
           FIAEYA LPLI P L PG  +     G+NFA+  AG    TFPG V  L +    Q++ F
Sbjct: 88  FIAEYAWLPLIPPNLQPGYSNSQLTYGLNFATTAAGVFAGTFPGSVTNLSKDLGTQLNNF 147

Query: 166 KETETSLRKKLGTSETKKLLSKAVYLIAIGSNDYGAFDPQSNLFYQSYTTQQYVDSVIGN 225
           K  E +LR  LG +E ++++SKAVYL  IG+NDY      +   + + T ++++D VIGN
Sbjct: 148 KNVEKTLRSNLGDAEARRVISKAVYLFHIGANDYQYPFFANTSTFSNTTKERFIDFVIGN 207

Query: 226 ITTVIKEIYKTGGRKFSVLNIGPIDHLPAVQEAIISHVRPLAWLEQFKRFIVLHNEQLPK 285
            TTVI+E+YK G RKF  L++GP    P+    II+  +  +  E     I LHN++ PK
Sbjct: 208 TTTVIEELYKLGARKFGFLSLGPFGCTPSA--LIINSTKIGSCFEPVTELINLHNQEFPK 267

Query: 286 ALQKLAQKLKGFS---------------------------ACCGSGALRGRDSCGGMKGI 345
            L++L ++L GF                            ACCGSG LRG ++CG   G 
Sbjct: 268 VLRRLERRLSGFKYALHDFHTSLSQRINNPSRYGFKEGEMACCGSGPLRGINTCGFRNGP 327

Query: 346 KE-YELCENPGEHVFFDANHGTDRIYQFIAEMMWTGTSNITTPINLNSLF 362
            + Y+LCEN  ++VFFD +H T+  +Q IAE++W+G  N+T P NL +LF
Sbjct: 328 SQGYKLCENADDYVFFDPSHLTETAHQQIAELIWSGPPNVTAPYNLKTLF 375

[Close]
BLAST of Lsi09G003010 vs. TAIR10
Match: AT1G53990.1 (AT1G53990.1 GDSL-motif lipase 3)

HSP 1 Score: 260.4 bits (664), Expect = 1.7e-69
Identity = 146/336 (43.45%), Postives = 202/336 (60.12%), Query Frame = 1 

Query: 56  LFVFGDSLYDVGNNNYINTTTISQANFPPYGQTFFKFPTGRFSDGRVIPDFIAEYAKLPL 115
           LFVFGDSL+D GNNNYINT +  ++N  PYGQT FKFPTGR SDG        E A LP 
Sbjct: 36  LFVFGDSLFDAGNNNYINTVSSFRSNIWPYGQTNFKFPTGRLSDG-------PEKAWLPS 95

Query: 116 ILPYLYP--GIKDFVKGVNFASGGAGALDQTFPGYVVPLRRQVDYFKETETSLRKKLGTS 175
           I P L P  G   F  GV+FAS GAGAL ++F G V+ L  Q++ FK+ E SLR +LG +
Sbjct: 96  IPPNLQPNNGNNQFTYGVSFASAGAGALAESFLGMVINLGTQLNNFKDVEKSLRSELGDA 155

Query: 176 ETKKLLSKAVYLIAIGSNDYGAFDPQSNLFYQSYTTQQYVDSVIGNITTVIKEIYKTGGR 235
           ETK++ S+AVYL  IG+NDY      ++  ++S + +++VD VIGNIT VI+E+YK GGR
Sbjct: 156 ETKRVFSRAVYLFHIGANDYFYPFSANSSTFKSNSKEKFVDFVIGNITFVIEEVYKMGGR 215

Query: 236 KFSVLNIGPIDHLPAVQEAIISHVRPLAWLEQFKRFIVLHNEQLPKALQKLAQKLKGF-- 295
           KF  LN+GP +  P     I    +  +  +     I +HN++ P  L++L ++L GF  
Sbjct: 216 KFGFLNVGPYECSP--NSLIRDRTKIGSCFKPVAELIDMHNKKFPDVLRRLQRQLSGFRY 275

Query: 296 -------------------------SACCGSGALRGRDSCGGMKGIKE-YELCENPGEHV 355
                                     ACCGSG LRG ++CG   G  + Y LCEN  +++
Sbjct: 276 ALHDYHTSLSERINSPSKYGFKEGKKACCGSGPLRGINTCGNRIGPSQGYGLCENVTDYL 335

Query: 356 FFDANHGTDRIYQFIAEMMWTGTSNITTPINLNSLF 362
           F+D++H T++ ++ IAE++W G  N+T P NL +LF
Sbjct: 336 FYDSSHLTEKAHRQIAELIWNGPPNVTRPYNLKALF 362

[Close]
BLAST of Lsi09G003010 vs. NCBI nr
Match: gi|449452488|ref|XP_004143991.1| (PREDICTED: GDSL esterase/lipase 1-like [Cucumis sativus])

HSP 1 Score: 582.4 bits (1500), Expect = 5.4e-163
Identity = 294/373 (78.82%), Postives = 311/373 (83.38%), Query Frame = 1 

Query: 19  MGNS-KLVTCGLLLLSVLGIIQMCEALGKQDEEEGEIPLFVFGDSLYDVGNNNYINTTTI 78
           M NS KLV CGLLLL  LGIIQ+CEALGK        PLFVFGDS+YDVGNNNYINTTTI
Sbjct: 2   MRNSIKLVLCGLLLLCSLGIIQICEALGKDH------PLFVFGDSIYDVGNNNYINTTTI 61

Query: 79  SQANFPPYGQTFFKFPTGRFSDGRVIPDFIAEYAKLPLILPYLYPGIKDFVKGVNFASGG 138
           SQANFPPYGQTFF+FPTGRFSDGRVIPDFIAEYAKLPLILPYLYPGIKDFVKGVNFASGG
Sbjct: 62  SQANFPPYGQTFFRFPTGRFSDGRVIPDFIAEYAKLPLILPYLYPGIKDFVKGVNFASGG 121

Query: 139 AGALDQTFPGYVVPLRRQVDYFKETETSLRKKLGTSETKKLLSKAVYLIAIGSNDYGAFD 198
           AG LD TFPGYVV LRRQV+YFKE E SLRKKLGTS+TKKLLSKAVYLIAIGS DY AFD
Sbjct: 122 AGVLDTTFPGYVVTLRRQVNYFKEMERSLRKKLGTSKTKKLLSKAVYLIAIGSGDYDAFD 181

Query: 199 PQSNLFYQSYTTQQYVDSVIGNITTVIKEIYKTGGRKFSVLNIGPIDHLPAVQEAIISHV 258
           P+SN  YQSYTTQQYVD VIGN+T+ I+EIYKTGGRKFSVLNIGPIDHLPAVQEAIISH 
Sbjct: 182 PKSNSLYQSYTTQQYVDLVIGNMTSFIEEIYKTGGRKFSVLNIGPIDHLPAVQEAIISHY 241

Query: 259 RPLAWLEQFKRFIVLHNEQLPKALQKLAQKLKGF-------------------------- 318
           R  AW+EQFK+FI LHNE+LPKALQ LAQK KG                           
Sbjct: 242 RTPAWMEQFKQFIGLHNEKLPKALQNLAQKFKGLLYSHTDFHTAISNIIHHPTKYGMKEV 301

Query: 319 -SACCGSGALRGRDSCGGMKGIKEYELCENPGEHVFFDANHGTDRIYQFIAEMMWTGTSN 364
            S CCGSGA RG+ SCGGM+GIKEYELCENP EHVFFDANHGTDRIY+F+AEMMWTGTSN
Sbjct: 302 KSGCCGSGAFRGKSSCGGMRGIKEYELCENPEEHVFFDANHGTDRIYKFVAEMMWTGTSN 361

[Close]
BLAST of Lsi09G003010 vs. NCBI nr
Match: gi|700194965|gb|KGN50142.1| (hypothetical protein Csa_5G155530 [Cucumis sativus])

HSP 1 Score: 442.6 bits (1137), Expect = 6.7e-121
Identity = 221/284 (77.82%), Postives = 235/284 (82.75%), Query Frame = 1 

Query: 107 IAEYAKLPLILPYLYPGIKDFVKGVNFASGGAGALDQTFPGYVVPLRRQVDYFKETETSL 166
           I+EYAKLPLILPYLYPGIKDFVKGVNFASGGAG LD TFPGYVV LRRQV+YFKE E SL
Sbjct: 21  ISEYAKLPLILPYLYPGIKDFVKGVNFASGGAGVLDTTFPGYVVTLRRQVNYFKEMERSL 80

Query: 167 RKKLGTSETKKLLSKAVYLIAIGSNDYGAFDPQSNLFYQSYTTQQYVDSVIGNITTVIKE 226
           RKKLGTS+TKKLLSKAVYLIAIGS DY AFDP+SN  YQSYTTQQYVD VIGN+T+ I+E
Sbjct: 81  RKKLGTSKTKKLLSKAVYLIAIGSGDYDAFDPKSNSLYQSYTTQQYVDLVIGNMTSFIEE 140

Query: 227 IYKTGGRKFSVLNIGPIDHLPAVQEAIISHVRPLAWLEQFKRFIVLHNEQLPKALQKLAQ 286
           IYKTGGRKFSVLNIGPIDHLPAVQEAIISH R  AW+EQFK+FI LHNE+LPKALQ LAQ
Sbjct: 141 IYKTGGRKFSVLNIGPIDHLPAVQEAIISHYRTPAWMEQFKQFIGLHNEKLPKALQNLAQ 200

Query: 287 KLKGF---------------------------SACCGSGALRGRDSCGGMKGIKEYELCE 346
           K KG                            S CCGSGA RG+ SCGGM+GIKEYELCE
Sbjct: 201 KFKGLLYSHTDFHTAISNIIHHPTKYGMKEVKSGCCGSGAFRGKSSCGGMRGIKEYELCE 260

Query: 347 NPGEHVFFDANHGTDRIYQFIAEMMWTGTSNITTPINLNSLFYI 364
           NP EHVFFDANHGTDRIY+F+AEMMWTGTSNITTPINLNSLFYI
Sbjct: 261 NPEEHVFFDANHGTDRIYKFVAEMMWTGTSNITTPINLNSLFYI 304

[Close]
BLAST of Lsi09G003010 vs. NCBI nr
Match: gi|449453393|ref|XP_004144442.1| (PREDICTED: GDSL esterase/lipase 1 [Cucumis sativus])

HSP 1 Score: 374.4 bits (960), Expect = 2.3e-100
Identity = 190/370 (51.35%), Postives = 250/370 (67.57%), Query Frame = 1 

Query: 19  MGNSKLVTCGLLLLSVLGIIQMCEALGKQDEEEGEIPLFVFGDSLYDVGNNNYINTTTIS 78
           M N K +   LLLL +   +  C ++ +QD     +PLFVFGDS+ D+GNNNYINT +I+
Sbjct: 1   MANLKDLCLCLLLLFIFFSVGDCISVCRQDHN---VPLFVFGDSIVDIGNNNYINTNSIA 60

Query: 79  QANFPPYGQTFFKFPTGRFSDGRVIPDFIAEYAKLPLILPYLYPGIKDFVKGVNFASGGA 138
           Q+N+PPYG TFFK+P+GR+SDGRV+PDF A+YA L L+LPYLYPG K ++ G+NFASGGA
Sbjct: 61  QSNYPPYGLTFFKYPSGRWSDGRVVPDFFAQYANLQLLLPYLYPGNKRYIDGINFASGGA 120

Query: 139 GALDQTFPGYVVPLRRQVDYFKETETSLRKKLGTSETKKLLSKAVYLIAIGSNDYGAFDP 198
           GALD+   G V+ L+ Q   FK+ E  LRK+LG ++ K LLS+AVYLI++G+NDY  F  
Sbjct: 121 GALDEINRGLVISLKTQARSFKKVEKILRKQLGKTQAKTLLSRAVYLISVGTNDYRTFAS 180

Query: 199 QSNLFYQSYTTQQYVDSVIGNITTVIKEIYKTGGRKFSVLNIGPIDHLPAVQEAIISHVR 258
            S LF  SY+ ++YVD VIGN+T+VIKEIYK GGRKF V+N+   +H+PAV EA+ S  +
Sbjct: 181 DSKLF-DSYSIEEYVDLVIGNLTSVIKEIYKNGGRKFVVMNLWSFNHVPAVLEAVASQGK 240

Query: 259 PLAWLEQFKRFIVLHNEQLPKAL-----------QKLAQKLKGF---------------- 318
             A L+Q  + + +HN+QL KAL                  K F                
Sbjct: 241 D-AQLKQLNQLVEMHNKQLYKALQKLTTELQGFRYSYVDSYKVFEEITTNPAKHGLKEVK 300

Query: 319 SACCGSGALRGRDSCGGMKGIKEYELCENPGEHVFFDANHGTDRIYQFIAEMMWTGTSNI 362
           SACCGSG  RG  SCGG   +KEYELC+NP EH+FFD+NHG+++ YQ +AEM W G SN 
Sbjct: 301 SACCGSGIYRGIQSCGGKGDVKEYELCKNPKEHLFFDSNHGSEKAYQILAEMAWNGDSNT 360

[Close]
BLAST of Lsi09G003010 vs. NCBI nr
Match: gi|659120754|ref|XP_008460337.1| (PREDICTED: GDSL esterase/lipase 1-like [Cucumis melo])

HSP 1 Score: 363.2 bits (931), Expect = 5.2e-97
Identity = 185/369 (50.14%), Postives = 244/369 (66.12%), Query Frame = 1 

Query: 20  GNSKLVTCGLLLLSVLGIIQMCEALGKQDEEEGEIPLFVFGDSLYDVGNNNYINTTTISQ 79
           G SK +    +++     +  C ++ KQD     +PLFVFGDS+ D+GNNNYINT  I++
Sbjct: 5   GESKRLVFLFIVVVHFFTVGDCISVCKQDHN---VPLFVFGDSIVDIGNNNYINTIPIAR 64

Query: 80  ANFPPYGQTFFKFPTGRFSDGRVIPDFIAEYAKLPLILPYLYPGIKDFVKGVNFASGGAG 139
           +N+PPYG TFFK P+GR+SDGRV+PDF A+YA L L+LPYLYPG K ++ G+NFASGGAG
Sbjct: 65  SNYPPYGLTFFKCPSGRWSDGRVVPDFFAQYANLQLLLPYLYPGNKHYINGINFASGGAG 124

Query: 140 ALDQTFPGYVVPLRRQVDYFKETETSLRKKLGTSETKKLLSKAVYLIAIGSNDYGAFDPQ 199
           ALD+   G V+ L+ Q   FK+ E  LRK++G ++ K LLS+AVYLI+IG+NDY  F   
Sbjct: 125 ALDEINRGLVISLKTQARSFKKVEKILRKQIGKTQAKALLSRAVYLISIGTNDYRTFASN 184

Query: 200 SNLFYQSYTTQQYVDSVIGNITTVIKEIYKTGGRKFSVLNIGPIDHLPAVQEAIISHVRP 259
           S LF  SY+ ++YVD VIGN+T+VIKEIYK GGRKF V+N+   +H+PAV EA+ S  + 
Sbjct: 185 SKLF-DSYSFEEYVDLVIGNLTSVIKEIYKNGGRKFVVMNLWSFNHVPAVLEAVASQGKD 244

Query: 260 LAWLEQFKRFIVLHNEQLPKAL-----------QKLAQKLKGF----------------S 319
            A LEQ  + + +HN+QL KA+                  K F                +
Sbjct: 245 -AQLEQLNQLVEMHNKQLYKAVQKLTTELQGFRYSYVDSYKVFEEITNNPAKHGLKEVKN 304

Query: 320 ACCGSGALRGRDSCGGMKGIKEYELCENPGEHVFFDANHGTDRIYQFIAEMMWTGTSNIT 362
           ACCGSG  RG  SCGG   +KEYELC NP EH+FFD+NHG+D+ YQ +AEM W G  N +
Sbjct: 305 ACCGSGVYRGIQSCGGKGNVKEYELCGNPKEHLFFDSNHGSDKAYQILAEMAWNGDLNAS 364

[Close]
BLAST of Lsi09G003010 vs. NCBI nr
Match: gi|225442009|ref|XP_002271802.1| (PREDICTED: GDSL esterase/lipase 1 [Vitis vinifera])

HSP 1 Score: 355.1 bits (910), Expect = 1.4e-94
Identity = 180/340 (52.94%), Postives = 225/340 (66.18%), Query Frame = 1 

Query: 49  EEEGEIPLFVFGDSLYDVGNNNYINTTTISQANFPPYGQTFFKFPTGRFSDGRVIPDFIA 108
           + +  +  F+FGDSL D GNNNYINTTT  QANF PYG+TFFK+PTGRFSDGR+IPDFIA
Sbjct: 33  QPQNHVAFFIFGDSLLDPGNNNYINTTTEDQANFRPYGETFFKYPTGRFSDGRLIPDFIA 92

Query: 109 EYAKLPLILPYLYPGIKDFVKGVNFASGGAGALDQTFPGYVVPLRRQVDYFKETETSLRK 168
           EYAKLPLI PYL PG   F  G NFASGGAGALD+   G VV L  Q+ YFK+ E  LR+
Sbjct: 93  EYAKLPLIPPYLQPGNHQFTYGANFASGGAGALDEINQGLVVNLNTQLRYFKKVEKHLRE 152

Query: 169 KLGTSETKKLLSKAVYLIAIGSNDYGAFDPQSNLFYQSYTTQQYVDSVIGNITTVIKEIY 228
           KLG  E+KKLL +AVYLI+IG NDY +   ++   +Q Y+ +QY+D V+GN+T VI+EIY
Sbjct: 153 KLGDEESKKLLLEAVYLISIGGNDYISPLFRNYSVFQIYSHRQYLDMVMGNLTVVIQEIY 212

Query: 229 KTGGRKFSVLNIGPIDHLPAVQEAIISHVRPLAWLEQFKRFIVLHNEQLPKALQKLAQKL 288
           + GGRKF  +N+GP+  LPA++   +        +E+    + LHN  LP+ LQKL  KL
Sbjct: 213 QKGGRKFGFVNMGPLGCLPAMKAIKLQQGGAGECMEEATVLVKLHNRVLPEVLQKLGSKL 272

Query: 289 KGFS---------------------------ACCGSGALRGRDSCGGMKGIKEYELCENP 348
           KGF                            ACCGSG  RG  SCGGM+G KEYELC N 
Sbjct: 273 KGFKYSIFDFYTTAKERMDNPSKYGFKEAKIACCGSGPYRGLYSCGGMRGTKEYELCSNV 332

Query: 349 GEHVFFDANHGTDRIYQFIAEMMWTGTSNITTPINLNSLF 362
            E++FFD+ H TDR+YQ +AE++W+GT N+  P NL  LF
Sbjct: 333 SEYMFFDSFHPTDRVYQQLAELVWSGTHNVIKPYNLKQLF 372

The following BLAST results are available for this feature:
BLAST of Lsi09G003010 vs. Swiss-Prot
Match NameE-valueIdentityDescription
GLIP1_ARATH1.2e-8245.99GDSL esterase/lipase 1 OS=Arabidopsis thaliana GN=GLIP1 PE=1 SV=1[more]
GLIP2_ARATH1.2e-7748.07GDSL esterase/lipase 2 OS=Arabidopsis thaliana GN=GLIP2 PE=2 SV=1[more]
GLIP5_ARATH1.6e-7747.21GDSL esterase/lipase 5 OS=Arabidopsis thaliana GN=GLIP5 PE=2 SV=2[more]
GRIP4_ARATH1.2e-7242.57GDSL esterase/lipase 4 OS=Arabidopsis thaliana GN=GLIP4 PE=2 SV=2[more]
GLIP3_ARATH3.0e-6843.45GDSL esterase/lipase 3 OS=Arabidopsis thaliana GN=GLIP3 PE=2 SV=2[more]
BLAST of Lsi09G003010 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KP02_CUCSA4.7e-12177.82Uncharacterized protein OS=Cucumis sativus GN=Csa_5G155530 PE=4 SV=1[more]
D7TZL6_VITVI9.9e-9552.94Putative uncharacterized protein OS=Vitis vinifera GN=VIT_09s0002g00540 PE=4 SV=... [more]
D7TZL3_VITVI3.9e-9153.24Putative uncharacterized protein OS=Vitis vinifera GN=VIT_09s0002g00510 PE=4 SV=... [more]
A0A061E3A2_THECC7.3e-9047.84GDSL lipase 1, putative OS=Theobroma cacao GN=TCM_007501 PE=4 SV=1[more]
A0A067L971_JATCU2.0e-8747.84Uncharacterized protein OS=Jatropha curcas GN=JCGZ_24643 PE=4 SV=1[more]
BLAST of Lsi09G003010 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G40990.17.0e-8445.99 GDSL lipase 1[more]
AT1G53920.18.8e-7947.21 GDSL-motif lipase 5[more]
AT1G53940.13.5e-7547.85 GDSL-motif lipase 2[more]
AT3G14225.16.6e-7442.57 GDSL-motif lipase 4[more]
AT1G53990.11.7e-6943.45 GDSL-motif lipase 3[more]
BLAST of Lsi09G003010 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449452488|ref|XP_004143991.1|5.4e-16378.82PREDICTED: GDSL esterase/lipase 1-like [Cucumis sativus][more]
gi|700194965|gb|KGN50142.1|6.7e-12177.82hypothetical protein Csa_5G155530 [Cucumis sativus][more]
gi|449453393|ref|XP_004144442.1|2.3e-10051.35PREDICTED: GDSL esterase/lipase 1 [Cucumis sativus][more]
gi|659120754|ref|XP_008460337.1|5.2e-9750.14PREDICTED: GDSL esterase/lipase 1-like [Cucumis melo][more]
gi|225442009|ref|XP_002271802.1|1.4e-9452.94PREDICTED: GDSL esterase/lipase 1 [Vitis vinifera][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0016788hydrolase activity, acting on ester bonds
Vocabulary: INTERPRO
TermDefinition
IPR001087GDSL
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006506 GPI anchor biosynthetic process
biological_process GO:0006629 lipid metabolic process
biological_process GO:0008152 metabolic process
biological_process GO:0016255 attachment of GPI anchor to protein
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005575 cellular_component
cellular_component GO:0042765 GPI-anchor transamidase complex
molecular_function GO:0016788 hydrolase activity, acting on ester bonds
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi09G003010.1Lsi09G003010.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 56..282
score: 7.5
NoneNo IPR availablePANTHERPTHR22835ZINC FINGER FYVE DOMAIN CONTAINING PROTEINcoord: 29..360
score: 3.2E
NoneNo IPR availablePANTHERPTHR22835:SF262GDSL ESTERASE/LIPASE 5-RELATEDcoord: 29..360
score: 3.2E

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Lsi09G003010Csa5G155530Cucumber (Chinese Long) v2culsiB322
Lsi09G003010CSPI05G05830Wild cucumber (PI 183967)cpilsiB322
Lsi09G003010CmaCh02G014800Cucurbita maxima (Rimu)cmalsiB530
Lsi09G003010CmoCh02G015180Cucurbita moschata (Rifu)cmolsiB516
Lsi09G003010Cla011483Watermelon (97103) v1lsiwmB027
Lsi09G003010ClCG01G003090Watermelon (Charleston Gray)lsiwcgB008
Lsi09G003010Cla97C01G003170Watermelon (97103) v2lsiwmbB007
Lsi09G003010CsGy5G003100Cucumber (Gy14) v2cgyblsiB294
Lsi09G003010CsaV3_5G003210Cucumber (Chinese Long) v3cuclsiB345
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Lsi09G003010Bottle gourd (USVL1VR-Ls)lsilsiB008
Lsi09G003010Cucumber (Gy14) v1cgylsiB411
Lsi09G003010Cucumber (Gy14) v1cgylsiB487
Lsi09G003010Cucurbita maxima (Rimu)cmalsiB267
Lsi09G003010Cucurbita maxima (Rimu)cmalsiB302
Lsi09G003010Cucurbita maxima (Rimu)cmalsiB365
Lsi09G003010Cucurbita moschata (Rifu)cmolsiB255
Lsi09G003010Cucurbita moschata (Rifu)cmolsiB290
Lsi09G003010Cucurbita moschata (Rifu)cmolsiB353
Lsi09G003010Cucurbita pepo (Zucchini)cpelsiB000
Lsi09G003010Cucurbita pepo (Zucchini)cpelsiB131
Lsi09G003010Cucurbita pepo (Zucchini)cpelsiB582
Lsi09G003010Wild cucumber (PI 183967)cpilsiB155
Lsi09G003010Cucumber (Chinese Long) v2culsiB156
Lsi09G003010Melon (DHL92) v3.5.1lsimeB012
Lsi09G003010Melon (DHL92) v3.5.1lsimeB036
Lsi09G003010Watermelon (Charleston Gray)lsiwcgB023
Lsi09G003010Watermelon (97103) v1lsiwmB024
Lsi09G003010Cucumber (Gy14) v2cgyblsiB141
Lsi09G003010Melon (DHL92) v3.6.1lsimedB012
Lsi09G003010Melon (DHL92) v3.6.1lsimedB036
Lsi09G003010Silver-seed gourdcarlsiB836
Lsi09G003010Silver-seed gourdcarlsiB851
Lsi09G003010Cucumber (Chinese Long) v3cuclsiB171
Lsi09G003010Watermelon (97103) v2lsiwmbB022
Lsi09G003010Wax gourdlsiwgoB022
Lsi09G003010Wax gourdlsiwgoB044