Lsi09G000540 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi09G000540
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Acidic chitinase
Location	chr09 : 446418 .. 446990 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCCCACAAAATTATCACAACCCTTTCAATAATCTTCCTCCTCTCCTCCATATTTCAATCTTCCGACGCGGCCGGAATTGCCATCTATTGGGGCCAAAACGGCAACGAAGGCTCTCTTGCCTCCACCTGCGCCACTGGAAACTACGAGTTTGTCAACGTAGCATTTCTCTCGTCTTTTGGCAACGGTCAAACTCCGGTCCTCAACCTTGCCGGTCATTGCAACCCTGACAACAACGGTTGCACTTTTTTGAGCGACGAAATAAACTCCTGCCAAAGTCAAGGCGTCAAAGTCCTCCTCTCTATCGGTGGTGGCGCAGGGAGCTATTCACTCTCCTCCGCCGACGATGCGAGAGAAGTCGCCAACTTTCTTTGGAACAACTTCCTCGGCGGGCAGTCGGGTTCAAGGCCACTTGGCGACGCCGTTTTGGACGGCATTGATTTCGATATCGAATCTGGCTCCGGTCAGTTCTGGGACGAACTAGCTCGACAGTTGAAAAGCTTCGATCAAGTCCTTCTCTCTGCCGCGCCGCAGTGTCCGATCCCCGACGCAAACCTAGACGCGGCCTAG

mRNA sequence

ATGGCTTCCCACAAAATTATCACAACCCTTTCAATAATCTTCCTCCTCTCCTCCATATTTCAATCTTCCGACGCGGCCGGAATTGCCATCTATTGGGGCCAAAACGGCAACGAAGGCTCTCTTGCCTCCACCTGCGCCACTGGAAACTACGAGTTTGTCAACGTAGCATTTCTCTCGTCTTTTGGCAACGGTCAAACTCCGGTCCTCAACCTTGCCGGTCATTGCAACCCTGACAACAACGGTTGCACTTTTTTGAGCGACGAAATAAACTCCTGCCAAAGTCAAGGCGTCAAAGTCCTCCTCTCTATCGGTGGTGGCGCAGGGAGCTATTCACTCTCCTCCGCCGACGATGCGAGAGAAGTCGCCAACTTTCTTTGGAACAACTTCCTCGGCGGGCAGTCGGGTTCAAGGCCACTTGGCGACGCCGTTTTGGACGGCATTGATTTCGATATCGAATCTGGCTCCGGTCAGTTCTGGGACGAACTAGCTCGACAGTTGAAAAGCTTCGATCAAGTCCTTCTCTCTGCCGCGCCGCAGTGTCCGATCCCCGACGCAAACCTAGACGCGGCCTAG

Coding sequence (CDS)

ATGGCTTCCCACAAAATTATCACAACCCTTTCAATAATCTTCCTCCTCTCCTCCATATTTCAATCTTCCGACGCGGCCGGAATTGCCATCTATTGGGGCCAAAACGGCAACGAAGGCTCTCTTGCCTCCACCTGCGCCACTGGAAACTACGAGTTTGTCAACGTAGCATTTCTCTCGTCTTTTGGCAACGGTCAAACTCCGGTCCTCAACCTTGCCGGTCATTGCAACCCTGACAACAACGGTTGCACTTTTTTGAGCGACGAAATAAACTCCTGCCAAAGTCAAGGCGTCAAAGTCCTCCTCTCTATCGGTGGTGGCGCAGGGAGCTATTCACTCTCCTCCGCCGACGATGCGAGAGAAGTCGCCAACTTTCTTTGGAACAACTTCCTCGGCGGGCAGTCGGGTTCAAGGCCACTTGGCGACGCCGTTTTGGACGGCATTGATTTCGATATCGAATCTGGCTCCGGTCAGTTCTGGGACGAACTAGCTCGACAGTTGAAAAGCTTCGATCAAGTCCTTCTCTCTGCCGCGCCGCAGTGTCCGATCCCCGACGCAAACCTAGACGCGGCCTAG

Protein sequence

MASHKIITTLSIIFLLSSIFQSSDAAGIAIYWGQNGNEGSLASTCATGNYEFVNVAFLSSFGNGQTPVLNLAGHCNPDNNGCTFLSDEINSCQSQGVKVLLSIGGGAGSYSLSSADDAREVANFLWNNFLGGQSGSRPLGDAVLDGIDFDIESGSGQFWDELARQLKSFDQVLLSAAPQCPIPDANLDAA

Homology

[Close]

BLAST of Lsi09G000540 vs. Swiss-Prot
Match: CHIA_CUCSA (Acidic endochitinase OS=Cucumis sativus PE=1 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 6.9e-93
Identity = 165/190 (86.84%), Postives = 181/190 (95.26%), Query Frame = 1


Query: 1   MASHKIITTLSIIFLLSSIFQSSDAAGIAIYWGQNGNEGSLASTCATGNYEFVNVAFLSS 60
           MA+HKI TTLSI FLLSSIF+SSDAAGIAIYWGQNGNEGSLASTCATGNYEFVN+AFLSS
Sbjct: 1   MAAHKITTTLSIFFLLSSIFRSSDAAGIAIYWGQNGNEGSLASTCATGNYEFVNIAFLSS 60

Query: 61  FGNGQTPVLNLAGHCNPDNNGCTFLSDEINSCQSQGVKVLLSIGGGAGSYSLSSADDARE 120
           FG+GQ PVLNLAGHCNPDNNGC FLSDEINSC+SQ VKVLLSIGGGAGSYSLSSADDA++
Sbjct: 61  FGSGQAPVLNLAGHCNPDNNGCAFLSDEINSCKSQNVKVLLSIGGGAGSYSLSSADDAKQ 120

Query: 121 VANFLWNNFLGGQSGSRPLGDAVLDGIDFDIESGSGQFWDELARQLKSFDQVLLSAAPQC 180
           VANF+WN++LGGQS SRPLG AVLDG+DFDIESGSGQFWD LA++LK+F QV+LSAAPQC
Sbjct: 121 VANFIWNSYLGGQSDSRPLGAAVLDGVDFDIESGSGQFWDVLAQELKNFGQVILSAAPQC 180

Query: 181 PIPDANLDAA 191
           PIPDA+LDAA
Sbjct: 181 PIPDAHLDAA 190

[Close]

BLAST of Lsi09G000540 vs. Swiss-Prot
Match: CHIA_PHAAN (Acidic endochitinase OS=Phaseolus angularis PE=2 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 3.3e-71
Identity = 127/191 (66.49%), Postives = 155/191 (81.15%), Query Frame = 1


Query: 1   MASHKIITTLSIIFLLSSIFQSSDAAGIAIYWGQNGNEGSLASTCATGNYEFVNVAFLSS 60
           MA  K ++ L +  L  S F+ S A GI++YWGQNGNEGSLA  C TGNY++VN+AFL +
Sbjct: 5   MACLKQVSALLLPLLFISFFKPSHAGGISVYWGQNGNEGSLADACNTGNYKYVNIAFLFT 64

Query: 61  FGNGQTPVLNLAGHCNPDNNGCTFLSDEINSCQSQGVKVLLSIGGGAGSYSLSSADDARE 120
           FG GQTP LNLAGHCNP  N C   SD+I  CQS+ +KVLLS+GG +GSYSL+SADDA +
Sbjct: 65  FGGGQTPQLNLAGHCNPSINNCNVFSDQIKECQSKDIKVLLSLGGASGSYSLTSADDATQ 124

Query: 121 VANFLWNNFLGGQSGSRPLGDAVLDGIDFDIESGSGQFWDELARQLKSFD-QVLLSAAPQ 180
           VAN++WNNFLGGQS SRPLGDA+LDG+DFDIESG+G+ WD+LAR LK F+ Q+LL+AAPQ
Sbjct: 125 VANYIWNNFLGGQSSSRPLGDAILDGVDFDIESGTGEHWDDLARALKGFNSQLLLTAAPQ 184

Query: 181 CPIPDANLDAA 191
           CPIPDA+LD A
Sbjct: 185 CPIPDAHLDTA 195

[Close]

BLAST of Lsi09G000540 vs. Swiss-Prot
Match: CHIA_CICAR (Acidic endochitinase OS=Cicer arietinum PE=2 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 5.9e-68
Identity = 126/188 (67.02%), Postives = 152/188 (80.85%), Query Frame = 1


Query: 5   KIITTLSIIFLLSSIFQSSDAAGIAIYWGQNGNEGSLASTCATGNYEFVNVAFLSSFGNG 64
           K    +  + L+S + +SS+AAGIA+YWGQNGNEGSL   C T NY+FVN+AFLS+FGNG
Sbjct: 3   KCFNIIPSLLLISLLIKSSNAAGIAVYWGQNGNEGSLQDACNTNNYQFVNIAFLSTFGNG 62

Query: 65  QTPVLNLAGHCNPDNNGCTFLSDEINSCQSQGVKVLLSIGGGAGSYSLSSADDAREVANF 124
           Q P +NLAGHC+P  NGCT  S EI +CQ++G+KVLLS+GGGAGSYSL+SA++A  +AN+
Sbjct: 63  QNPQINLAGHCDPSTNGCTKFSPEIQACQAKGIKVLLSLGGGAGSYSLNSAEEATTLANY 122

Query: 125 LWNNFLGGQSGSRPLGDAVLDGIDFDIESGSGQFWDELARQLKSFDQ--VLLSAAPQCPI 184
           LWNNFLGG S SRPLGDAVLDGIDFDIESG GQ +DELA+ L  F Q  V LSAAPQCP 
Sbjct: 123 LWNNFLGGTSTSRPLGDAVLDGIDFDIESG-GQHYDELAKALNGFSQQKVYLSAAPQCPY 182

Query: 185 PDANLDAA 191
           PDA+LD+A
Sbjct: 183 PDAHLDSA 189

[Close]

BLAST of Lsi09G000540 vs. Swiss-Prot
Match: CHIA_TOBAC (Acidic endochitinase OS=Nicotiana tabacum PE=1 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 1.6e-65
Identity = 123/193 (63.73%), Postives = 147/193 (76.17%), Query Frame = 1


Query: 1   MASHKIITTLSIIFLLSSIFQSSDAAGIAIYWGQNGNEGSLASTCATGNYEFVNVAFLSS 60
           M  +  + T  ++FL +   ++ D   I IYWGQNGNEGSLA TCAT NY  VN+AFL  
Sbjct: 1   MIKYSFLLTALVLFLRALKLEAGD---IVIYWGQNGNEGSLADTCATNNYAIVNIAFLVV 60

Query: 61  FGNGQTPVLNLAGHCNPDNNGCTFLSDEINSCQSQGVKVLLSIGGGAGSYSLSSADDARE 120
           FGNGQ PVLNLAGHC+P+   CT LS++I +CQ+QG+KV+LS+GGGAGSY LSSADDAR 
Sbjct: 61  FGNGQNPVLNLAGHCDPNAGACTGLSNDIRACQNQGIKVMLSLGGGAGSYFLSSADDARN 120

Query: 121 VANFLWNNFLGGQSGSRPLGDAVLDGIDFDIESGSGQFWDELARQLKSFDQ---VLLSAA 180
           VAN+LWNN+LGGQS +RPLGDAVLDGIDFDIE G+ Q WDELA+ L  F Q   V L+AA
Sbjct: 121 VANYLWNNYLGGQSNTRPLGDAVLDGIDFDIEGGTTQHWDELAKTLSQFSQQRKVYLTAA 180

Query: 181 PQCPIPDANLDAA 191
           PQCP PD  L+ A
Sbjct: 181 PQCPFPDTWLNGA 190

[Close]

BLAST of Lsi09G000540 vs. Swiss-Prot
Match: CHIT3_VITVI (Acidic endochitinase OS=Vitis vinifera GN=CHIT3 PE=2 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 8.0e-65
Identity = 122/189 (64.55%), Postives = 148/189 (78.31%), Query Frame = 1


Query: 3   SHKIITTLSIIFLLSSIFQSSDAAGIAIYWGQNGNEGSLASTCATGNYEFVNVAFLSSFG 62
           S  ++ +LS++ LL    Q+S A GIAIYWGQNGNEG+L  TC TG Y +VN+AFL+ FG
Sbjct: 7   STPLLISLSVLALL----QTSYAGGIAIYWGQNGNEGTLTQTCNTGKYSYVNIAFLNKFG 66

Query: 63  NGQTPVLNLAGHCNPDNNGCTFLSDEINSCQSQGVKVLLSIGGGAGSYSLSSADDAREVA 122
           NGQTP +NLAGHCNP +NGCT +S  I +CQ++G+KV+LSIGGGAGSYSLSS++DA+ VA
Sbjct: 67  NGQTPEINLAGHCNPASNGCTSVSTGIRNCQNRGIKVMLSIGGGAGSYSLSSSNDAQNVA 126

Query: 123 NFLWNNFLGGQSGSRPLGDAVLDGIDFDIESGSGQFWDELARQLKSFD-------QVLLS 182
           N+LWNNFLGGQS SRPLGDAVLDGIDFDIE GS   WD+LAR L   +       +V L+
Sbjct: 127 NYLWNNFLGGQSSSRPLGDAVLDGIDFDIELGSTLHWDDLARALSRIEFQQERGRKVYLT 186

Query: 183 AAPQCPIPD 185
           AAPQCP PD
Sbjct: 187 AAPQCPFPD 191

[Close]

BLAST of Lsi09G000540 vs. TrEMBL
Match: Q09Y38_CITLA (Acidic class III chitinase OS=Citrullus lanatus PE=2 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 1.8e-95
Identity = 175/190 (92.11%), Postives = 184/190 (96.84%), Query Frame = 1


Query: 1   MASHKIITTLSIIFLLSSIFQSSDAAGIAIYWGQNGNEGSLASTCATGNYEFVNVAFLSS 60
           MASHKI TTLSIIFLLSSIF+SSDAAGIAIYWGQNGNEGSLASTCATGNY+FVN+AFLSS
Sbjct: 1   MASHKITTTLSIIFLLSSIFRSSDAAGIAIYWGQNGNEGSLASTCATGNYKFVNIAFLSS 60

Query: 61  FGNGQTPVLNLAGHCNPDNNGCTFLSDEINSCQSQGVKVLLSIGGGAGSYSLSSADDARE 120
           FGNGQTPVLNLAGHCNPDNNGC FLSDEINSC+S G+KVLLSIGGGAGSYSLSSA+DAR+
Sbjct: 61  FGNGQTPVLNLAGHCNPDNNGCAFLSDEINSCKSLGIKVLLSIGGGAGSYSLSSAEDARD 120

Query: 121 VANFLWNNFLGGQSGSRPLGDAVLDGIDFDIESGSGQFWDELARQLKSFDQVLLSAAPQC 180
           VANFLWNNFLGGQS SRPLGDAVLDGIDFDIESGSGQ+WDELARQLK F QVLLSAAPQC
Sbjct: 121 VANFLWNNFLGGQSSSRPLGDAVLDGIDFDIESGSGQWWDELARQLKGFGQVLLSAAPQC 180

Query: 181 PIPDANLDAA 191
           PIPDA+LDAA
Sbjct: 181 PIPDAHLDAA 190

[Close]

BLAST of Lsi09G000540 vs. TrEMBL
Match: I3RVA1_9ROSI (Acidic class III chitinase OS=Citrullus colocynthis PE=3 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 3.0e-95
Identity = 174/190 (91.58%), Postives = 183/190 (96.32%), Query Frame = 1


Query: 1   MASHKIITTLSIIFLLSSIFQSSDAAGIAIYWGQNGNEGSLASTCATGNYEFVNVAFLSS 60
           MA+HKI TTLSIIFLLSSI +SSDAAGIAIYWGQNGNEGSLASTCATGNY+FVN+AFLSS
Sbjct: 1   MAAHKITTTLSIIFLLSSILRSSDAAGIAIYWGQNGNEGSLASTCATGNYKFVNIAFLSS 60

Query: 61  FGNGQTPVLNLAGHCNPDNNGCTFLSDEINSCQSQGVKVLLSIGGGAGSYSLSSADDARE 120
           FGNGQTPVLNLAGHCNPDNNGC FLSDEINSC+SQG+KVLLSIGGGAGSYSLSS DDAR+
Sbjct: 61  FGNGQTPVLNLAGHCNPDNNGCAFLSDEINSCKSQGIKVLLSIGGGAGSYSLSSTDDARD 120

Query: 121 VANFLWNNFLGGQSGSRPLGDAVLDGIDFDIESGSGQFWDELARQLKSFDQVLLSAAPQC 180
           VANFLWNNFLGGQS SRPLGDAVLDGIDFDIESGSGQ+WDELARQLK F QVLLSAAPQC
Sbjct: 121 VANFLWNNFLGGQSSSRPLGDAVLDGIDFDIESGSGQWWDELARQLKGFGQVLLSAAPQC 180

Query: 181 PIPDANLDAA 191
           PIPDA+LDAA
Sbjct: 181 PIPDAHLDAA 190

[Close]

BLAST of Lsi09G000540 vs. TrEMBL
Match: Q9M544_CUCME (Chitinase 1 OS=Cucumis melo PE=2 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 7.0e-92
Identity = 169/190 (88.95%), Postives = 182/190 (95.79%), Query Frame = 1


Query: 1   MASHKIITTLSIIFLLSSIFQSSDAAGIAIYWGQNGNEGSLASTCATGNYEFVNVAFLSS 60
           MA+HKI TTLSI FLLSSIF+SS+AAGIAIYWGQNGNEGSLASTCATGNYEFVN+AFLSS
Sbjct: 1   MAAHKITTTLSIFFLLSSIFRSSEAAGIAIYWGQNGNEGSLASTCATGNYEFVNIAFLSS 60

Query: 61  FGNGQTPVLNLAGHCNPDNNGCTFLSDEINSCQSQGVKVLLSIGGGAGSYSLSSADDARE 120
           FG+GQTPVLNLAGHCNPDNNGC FLSDEINSCQSQ VKVLLSIGGGAGSYSLSSADDAR+
Sbjct: 61  FGSGQTPVLNLAGHCNPDNNGCAFLSDEINSCQSQNVKVLLSIGGGAGSYSLSSADDARQ 120

Query: 121 VANFLWNNFLGGQSGSRPLGDAVLDGIDFDIESGSGQFWDELARQLKSFDQVLLSAAPQC 180
           VANFLWN++LGGQS SRPLG AVL+GIDFDIESGSGQFWD LA++LKSF QV+LSAAPQC
Sbjct: 121 VANFLWNSYLGGQSDSRPLGAAVLNGIDFDIESGSGQFWDVLAQELKSFGQVILSAAPQC 180

Query: 181 PIPDANLDAA 191
           PIPDA+LDAA
Sbjct: 181 PIPDAHLDAA 190

[Close]

BLAST of Lsi09G000540 vs. TrEMBL
Match: D6QXZ9_CUCSA (Chitinase OS=Cucumis sativus GN=Csa_5G139760 PE=2 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 1.0e-90
Identity = 165/190 (86.84%), Postives = 180/190 (94.74%), Query Frame = 1


Query: 1   MASHKIITTLSIIFLLSSIFQSSDAAGIAIYWGQNGNEGSLASTCATGNYEFVNVAFLSS 60
           MA+HKI TTLSI FLLSSIF+SSDAAGIAIYWGQNGNEGSLASTCATGNYEFVN+AFLSS
Sbjct: 1   MAAHKITTTLSIFFLLSSIFRSSDAAGIAIYWGQNGNEGSLASTCATGNYEFVNIAFLSS 60

Query: 61  FGNGQTPVLNLAGHCNPDNNGCTFLSDEINSCQSQGVKVLLSIGGGAGSYSLSSADDARE 120
           FG+GQ PVLNLAGHCNPDNNGC FLSDEINSC+SQ VKVLLSIGGGAGSYSLSSADDA++
Sbjct: 61  FGSGQAPVLNLAGHCNPDNNGCAFLSDEINSCKSQNVKVLLSIGGGAGSYSLSSADDAKQ 120

Query: 121 VANFLWNNFLGGQSGSRPLGDAVLDGIDFDIESGSGQFWDELARQLKSFDQVLLSAAPQC 180
           VANF+WN++LGGQS SRPLG AVLDG+DFDIESGSGQFWD LA++LK+F QV+LSAAPQC
Sbjct: 121 VANFIWNSYLGGQSDSRPLGAAVLDGVDFDIESGSGQFWDVLAQELKNFGQVILSAAPQC 180

Query: 181 PIPDANLDAA 191
           PIPDA LDAA
Sbjct: 181 PIPDAQLDAA 190

[Close]

BLAST of Lsi09G000540 vs. TrEMBL
Match: A0A088GK18_CUCMA (Acidic class III chitinase OS=Cucurbita maxima PE=3 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 2.9e-90
Identity = 164/190 (86.32%), Postives = 181/190 (95.26%), Query Frame = 1


Query: 1   MASHKIITTLSIIFLLSSIFQSSDAAGIAIYWGQNGNEGSLASTCATGNYEFVNVAFLSS 60
           MA+HKI TTLSI FLLSSIF+SSDAAGIAIYWGQNGNEGSLASTCATGNYEFVN+AFLSS
Sbjct: 1   MAAHKITTTLSIFFLLSSIFRSSDAAGIAIYWGQNGNEGSLASTCATGNYEFVNIAFLSS 60

Query: 61  FGNGQTPVLNLAGHCNPDNNGCTFLSDEINSCQSQGVKVLLSIGGGAGSYSLSSADDARE 120
           FG+GQ PVLNLAGHCNPDNNGC FLSDEINSC+SQ VKVLLSIGGGAGSYSLSSADDA++
Sbjct: 61  FGSGQAPVLNLAGHCNPDNNGCAFLSDEINSCKSQNVKVLLSIGGGAGSYSLSSADDAKQ 120

Query: 121 VANFLWNNFLGGQSGSRPLGDAVLDGIDFDIESGSGQFWDELARQLKSFDQVLLSAAPQC 180
           VANF+WN++LGGQS SRPLG AVL+G+DFDIESGSGQFWD LA++LK+F QV+LSAAPQC
Sbjct: 121 VANFIWNSYLGGQSDSRPLGAAVLNGVDFDIESGSGQFWDVLAQELKNFGQVILSAAPQC 180

Query: 181 PIPDANLDAA 191
           PIPDA+LDAA
Sbjct: 181 PIPDAHLDAA 190

[Close]

BLAST of Lsi09G000540 vs. TAIR10
Match: AT5G24090.1 (AT5G24090.1 chitinase A)

HSP 1 Score: 236.9 bits (603), Expect = 1.0e-62
Identity = 112/185 (60.54%), Postives = 142/185 (76.76%), Query Frame = 1


Query: 12  IIFLLSSIFQSSDAA--GIAIYWGQNGNEGSLASTCATGNYEFVNVAFLSSFGNGQTPVL 71
           + F+  S+ + SDA+  GIAIYWGQNGNEG+L++TCATG Y +VNVAFL  FGNGQTP L
Sbjct: 14  LFFISCSLSKPSDASRGGIAIYWGQNGNEGNLSATCATGRYAYVNVAFLVKFGNGQTPEL 73

Query: 72  NLAGHCNPDNNGCTFLSDEINSCQSQGVKVLLSIGGGAGSYSLSSADDAREVANFLWNNF 131
           NLAGHCNP  N CT    ++  CQS+G+KV+LS+GGG G+YS+ S +DA+ +A++LWNNF
Sbjct: 74  NLAGHCNPAANTCTHFGSQVKDCQSRGIKVMLSLGGGIGNYSIGSREDAKVIADYLWNNF 133

Query: 132 LGGQSGSRPLGDAVLDGIDFDIESGSGQFWDELARQLKSFD----QVLLSAAPQCPIPDA 191
           LGG+S SRPLGDAVLDGIDF+IE GS Q WD+LAR L  F     ++ L+ APQCP PD 
Sbjct: 134 LGGKSSSRPLGDAVLDGIDFNIELGSPQHWDDLARTLSKFSHRGRKIYLTGAPQCPFPDR 193

[Close]

BLAST of Lsi09G000540 vs. NCBI nr
Match: gi|75708015|gb|ABA26457.1| (acidic class III chitinase [Citrullus lanatus])

HSP 1 Score: 356.7 bits (914), Expect = 2.5e-95
Identity = 175/190 (92.11%), Postives = 184/190 (96.84%), Query Frame = 1


Query: 1   MASHKIITTLSIIFLLSSIFQSSDAAGIAIYWGQNGNEGSLASTCATGNYEFVNVAFLSS 60
           MASHKI TTLSIIFLLSSIF+SSDAAGIAIYWGQNGNEGSLASTCATGNY+FVN+AFLSS
Sbjct: 1   MASHKITTTLSIIFLLSSIFRSSDAAGIAIYWGQNGNEGSLASTCATGNYKFVNIAFLSS 60

Query: 61  FGNGQTPVLNLAGHCNPDNNGCTFLSDEINSCQSQGVKVLLSIGGGAGSYSLSSADDARE 120
           FGNGQTPVLNLAGHCNPDNNGC FLSDEINSC+S G+KVLLSIGGGAGSYSLSSA+DAR+
Sbjct: 61  FGNGQTPVLNLAGHCNPDNNGCAFLSDEINSCKSLGIKVLLSIGGGAGSYSLSSAEDARD 120

Query: 121 VANFLWNNFLGGQSGSRPLGDAVLDGIDFDIESGSGQFWDELARQLKSFDQVLLSAAPQC 180
           VANFLWNNFLGGQS SRPLGDAVLDGIDFDIESGSGQ+WDELARQLK F QVLLSAAPQC
Sbjct: 121 VANFLWNNFLGGQSSSRPLGDAVLDGIDFDIESGSGQWWDELARQLKGFGQVLLSAAPQC 180

Query: 181 PIPDANLDAA 191
           PIPDA+LDAA
Sbjct: 181 PIPDAHLDAA 190

[Close]

BLAST of Lsi09G000540 vs. NCBI nr
Match: gi|388458933|gb|AFK31317.1| (acidic class III chitinase [Citrullus colocynthis])

HSP 1 Score: 355.9 bits (912), Expect = 4.3e-95
Identity = 174/190 (91.58%), Postives = 183/190 (96.32%), Query Frame = 1


Query: 1   MASHKIITTLSIIFLLSSIFQSSDAAGIAIYWGQNGNEGSLASTCATGNYEFVNVAFLSS 60
           MA+HKI TTLSIIFLLSSI +SSDAAGIAIYWGQNGNEGSLASTCATGNY+FVN+AFLSS
Sbjct: 1   MAAHKITTTLSIIFLLSSILRSSDAAGIAIYWGQNGNEGSLASTCATGNYKFVNIAFLSS 60

Query: 61  FGNGQTPVLNLAGHCNPDNNGCTFLSDEINSCQSQGVKVLLSIGGGAGSYSLSSADDARE 120
           FGNGQTPVLNLAGHCNPDNNGC FLSDEINSC+SQG+KVLLSIGGGAGSYSLSS DDAR+
Sbjct: 61  FGNGQTPVLNLAGHCNPDNNGCAFLSDEINSCKSQGIKVLLSIGGGAGSYSLSSTDDARD 120

Query: 121 VANFLWNNFLGGQSGSRPLGDAVLDGIDFDIESGSGQFWDELARQLKSFDQVLLSAAPQC 180
           VANFLWNNFLGGQS SRPLGDAVLDGIDFDIESGSGQ+WDELARQLK F QVLLSAAPQC
Sbjct: 121 VANFLWNNFLGGQSSSRPLGDAVLDGIDFDIESGSGQWWDELARQLKGFGQVLLSAAPQC 180

Query: 181 PIPDANLDAA 191
           PIPDA+LDAA
Sbjct: 181 PIPDAHLDAA 190

[Close]

BLAST of Lsi09G000540 vs. NCBI nr
Match: gi|7595839|gb|AAF64474.1|AF241266_1 (chitinase 1 [Cucumis melo])

HSP 1 Score: 344.7 bits (883), Expect = 1.0e-91
Identity = 169/190 (88.95%), Postives = 182/190 (95.79%), Query Frame = 1


Query: 1   MASHKIITTLSIIFLLSSIFQSSDAAGIAIYWGQNGNEGSLASTCATGNYEFVNVAFLSS 60
           MA+HKI TTLSI FLLSSIF+SS+AAGIAIYWGQNGNEGSLASTCATGNYEFVN+AFLSS
Sbjct: 1   MAAHKITTTLSIFFLLSSIFRSSEAAGIAIYWGQNGNEGSLASTCATGNYEFVNIAFLSS 60

Query: 61  FGNGQTPVLNLAGHCNPDNNGCTFLSDEINSCQSQGVKVLLSIGGGAGSYSLSSADDARE 120
           FG+GQTPVLNLAGHCNPDNNGC FLSDEINSCQSQ VKVLLSIGGGAGSYSLSSADDAR+
Sbjct: 61  FGSGQTPVLNLAGHCNPDNNGCAFLSDEINSCQSQNVKVLLSIGGGAGSYSLSSADDARQ 120

Query: 121 VANFLWNNFLGGQSGSRPLGDAVLDGIDFDIESGSGQFWDELARQLKSFDQVLLSAAPQC 180
           VANFLWN++LGGQS SRPLG AVL+GIDFDIESGSGQFWD LA++LKSF QV+LSAAPQC
Sbjct: 121 VANFLWNSYLGGQSDSRPLGAAVLNGIDFDIESGSGQFWDVLAQELKSFGQVILSAAPQC 180

Query: 181 PIPDANLDAA 191
           PIPDA+LDAA
Sbjct: 181 PIPDAHLDAA 190

[Close]

BLAST of Lsi09G000540 vs. NCBI nr
Match: gi|659074352|ref|XP_008437557.1| (PREDICTED: acidic endochitinase [Cucumis melo])

HSP 1 Score: 344.7 bits (883), Expect = 1.0e-91
Identity = 169/190 (88.95%), Postives = 182/190 (95.79%), Query Frame = 1


Query: 1   MASHKIITTLSIIFLLSSIFQSSDAAGIAIYWGQNGNEGSLASTCATGNYEFVNVAFLSS 60
           MA+HKI TTLSI FLLSSIF+SS+AAGIAIYWGQNGNEGSLASTCATGNYEFVN+AFLSS
Sbjct: 1   MAAHKITTTLSIFFLLSSIFRSSEAAGIAIYWGQNGNEGSLASTCATGNYEFVNIAFLSS 60

Query: 61  FGNGQTPVLNLAGHCNPDNNGCTFLSDEINSCQSQGVKVLLSIGGGAGSYSLSSADDARE 120
           FG+GQTPVLNLAGHCNPDNNGC FLSDEINSCQSQ VKVLLSIGGGAGSYSLSSADDAR+
Sbjct: 61  FGSGQTPVLNLAGHCNPDNNGCAFLSDEINSCQSQNVKVLLSIGGGAGSYSLSSADDARQ 120

Query: 121 VANFLWNNFLGGQSGSRPLGDAVLDGIDFDIESGSGQFWDELARQLKSFDQVLLSAAPQC 180
           VANFLWN++LGGQS SRPLG AVL+GIDFDIESGSGQFWD LA++LKSF QV+LSAAPQC
Sbjct: 121 VANFLWNSYLGGQSDSRPLGAAVLNGIDFDIESGSGQFWDVLAQELKSFGQVILSAAPQC 180

Query: 181 PIPDANLDAA 191
           PIPDA+LDAA
Sbjct: 181 PIPDAHLDAA 190

[Close]

BLAST of Lsi09G000540 vs. NCBI nr
Match: gi|116328|sp|P17541.1|CHIA_CUCSA (RecName: Full=Acidic endochitinase; Flags: Precursor)

HSP 1 Score: 341.3 bits (874), Expect = 1.1e-90
Identity = 165/190 (86.84%), Postives = 181/190 (95.26%), Query Frame = 1


Query: 1   MASHKIITTLSIIFLLSSIFQSSDAAGIAIYWGQNGNEGSLASTCATGNYEFVNVAFLSS 60
           MA+HKI TTLSI FLLSSIF+SSDAAGIAIYWGQNGNEGSLASTCATGNYEFVN+AFLSS
Sbjct: 1   MAAHKITTTLSIFFLLSSIFRSSDAAGIAIYWGQNGNEGSLASTCATGNYEFVNIAFLSS 60

Query: 61  FGNGQTPVLNLAGHCNPDNNGCTFLSDEINSCQSQGVKVLLSIGGGAGSYSLSSADDARE 120
           FG+GQ PVLNLAGHCNPDNNGC FLSDEINSC+SQ VKVLLSIGGGAGSYSLSSADDA++
Sbjct: 61  FGSGQAPVLNLAGHCNPDNNGCAFLSDEINSCKSQNVKVLLSIGGGAGSYSLSSADDAKQ 120

Query: 121 VANFLWNNFLGGQSGSRPLGDAVLDGIDFDIESGSGQFWDELARQLKSFDQVLLSAAPQC 180
           VANF+WN++LGGQS SRPLG AVLDG+DFDIESGSGQFWD LA++LK+F QV+LSAAPQC
Sbjct: 121 VANFIWNSYLGGQSDSRPLGAAVLDGVDFDIESGSGQFWDVLAQELKNFGQVILSAAPQC 180

Query: 181 PIPDANLDAA 191
           PIPDA+LDAA
Sbjct: 181 PIPDAHLDAA 190

The following BLAST results are available for this feature:

BLAST of Lsi09G000540 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CHIA_CUCSA	6.9e-93	86.84	Acidic endochitinase OS=Cucumis sativus PE=1 SV=1	[more]
CHIA_PHAAN	3.3e-71	66.49	Acidic endochitinase OS=Phaseolus angularis PE=2 SV=1	[more]
CHIA_CICAR	5.9e-68	67.02	Acidic endochitinase OS=Cicer arietinum PE=2 SV=1	[more]
CHIA_TOBAC	1.6e-65	63.73	Acidic endochitinase OS=Nicotiana tabacum PE=1 SV=1	[more]
CHIT3_VITVI	8.0e-65	64.55	Acidic endochitinase OS=Vitis vinifera GN=CHIT3 PE=2 SV=1	[more]

BLAST of Lsi09G000540 vs. TrEMBL


Match Name	E-value	Identity	Description
Q09Y38_CITLA	1.8e-95	92.11	Acidic class III chitinase OS=Citrullus lanatus PE=2 SV=1	[more]
I3RVA1_9ROSI	3.0e-95	91.58	Acidic class III chitinase OS=Citrullus colocynthis PE=3 SV=1	[more]
Q9M544_CUCME	7.0e-92	88.95	Chitinase 1 OS=Cucumis melo PE=2 SV=1	[more]
D6QXZ9_CUCSA	1.0e-90	86.84	Chitinase OS=Cucumis sativus GN=Csa_5G139760 PE=2 SV=1	[more]
A0A088GK18_CUCMA	2.9e-90	86.32	Acidic class III chitinase OS=Cucurbita maxima PE=3 SV=1	[more]

BLAST of Lsi09G000540 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G24090.1	1.0e-62	60.54	chitinase A	[more]

BLAST of Lsi09G000540 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|75708015\|gb\|ABA26457.1\|	2.5e-95	92.11	acidic class III chitinase [Citrullus lanatus]	[more]
gi\|388458933\|gb\|AFK31317.1\|	4.3e-95	91.58	acidic class III chitinase [Citrullus colocynthis]	[more]
gi\|7595839\|gb\|AAF64474.1\|AF241266_1	1.0e-91	88.95	chitinase 1 [Cucumis melo]	[more]
gi\|659074352\|ref\|XP_008437557.1\|	1.0e-91	88.95	PREDICTED: acidic endochitinase [Cucumis melo]	[more]
gi\|116328\|sp\|P17541.1\|CHIA_CUCSA	1.1e-90	86.84	RecName: Full=Acidic endochitinase; Flags: Precursor	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0005975	carbohydrate metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds

Vocabulary: **INTERPRO**
Term	Definition
IPR017853	Glycoside_hydrolase_SF
IPR013781	Glycoside hydrolase, catalytic domain
IPR001579	Glyco_hydro_18_chit_AS
IPR001223	Glyco_hydro18_cat

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016998	cell wall macromolecule catabolic process
biological_process	GO:0006032	chitin catabolic process
biological_process	GO:0000272	polysaccharide catabolic process
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0005615	extracellular space
molecular_function	GO:0004568	chitinase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi09G000540.1	Lsi09G000540.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001223	Glycoside hydrolase family 18, catalytic domain	PFAM	PF00704	Glyco_hydro_18	coord: 28..184 score: 3.6
IPR001579	Glycoside hydrolase, chitinase active site	PROSITE	PS01095	CHITINASE_18	coord: 144..152 scor
IPR013781	Glycoside hydrolase, catalytic domain	GENE3D	G3DSA:3.20.20.80		coord: 24..189 score: 1.7
IPR017853	Glycoside hydrolase superfamily	unknown	SSF51445	(Trans)glycosidases	coord: 24..188 score: 1.87
None	No IPR available	PANTHER	PTHR11177	CHITINASE	coord: 1..190 score: 1.5E
None	No IPR available	PANTHER	PTHR11177:SF161	SUBFAMILY NOT NAMED	coord: 1..190 score: 1.5E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi09G000540	Bhi12G000526	Wax gourd	lsiwgoB024

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Lsi09G000540	Lsi05G020810	Bottle gourd (USVL1VR-Ls)	lsilsiB014

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi09G000540	Silver-seed gourd	carlsiB276
Lsi09G000540	Silver-seed gourd	carlsiB457
Lsi09G000540	Silver-seed gourd	carlsiB487
Lsi09G000540	Silver-seed gourd	carlsiB902
Lsi09G000540	Cucumber (Chinese Long) v3	cuclsiB164
Lsi09G000540	Cucumber (Chinese Long) v3	cuclsiB268
Lsi09G000540	Cucumber (Chinese Long) v3	cuclsiB345
Lsi09G000540	Watermelon (97103) v2	lsiwmbB007
Lsi09G000540	Watermelon (97103) v2	lsiwmbB019
Lsi09G000540	Wax gourd	lsiwgoB045
Lsi09G000540	Bottle gourd (USVL1VR-Ls)	lsilsiB010
Lsi09G000540	Cucumber (Gy14) v1	cgylsiB347
Lsi09G000540	Cucumber (Gy14) v1	cgylsiB413
Lsi09G000540	Cucurbita maxima (Rimu)	cmalsiB226
Lsi09G000540	Cucurbita maxima (Rimu)	cmalsiB262
Lsi09G000540	Cucurbita maxima (Rimu)	cmalsiB298
Lsi09G000540	Cucurbita maxima (Rimu)	cmalsiB363
Lsi09G000540	Cucurbita maxima (Rimu)	cmalsiB536
Lsi09G000540	Cucurbita moschata (Rifu)	cmolsiB214
Lsi09G000540	Cucurbita moschata (Rifu)	cmolsiB249
Lsi09G000540	Cucurbita moschata (Rifu)	cmolsiB286
Lsi09G000540	Cucurbita moschata (Rifu)	cmolsiB351
Lsi09G000540	Cucurbita moschata (Rifu)	cmolsiB520
Lsi09G000540	Cucurbita pepo (Zucchini)	cpelsiB019
Lsi09G000540	Cucurbita pepo (Zucchini)	cpelsiB163
Lsi09G000540	Cucurbita pepo (Zucchini)	cpelsiB134
Lsi09G000540	Cucurbita pepo (Zucchini)	cpelsiB486
Lsi09G000540	Cucurbita pepo (Zucchini)	cpelsiB579
Lsi09G000540	Wild cucumber (PI 183967)	cpilsiB150
Lsi09G000540	Wild cucumber (PI 183967)	cpilsiB322
Lsi09G000540	Cucumber (Chinese Long) v2	culsiB151
Lsi09G000540	Cucumber (Chinese Long) v2	culsiB322
Lsi09G000540	Melon (DHL92) v3.5.1	lsimeB012
Lsi09G000540	Melon (DHL92) v3.5.1	lsimeB031
Lsi09G000540	Melon (DHL92) v3.5.1	lsimeB035
Lsi09G000540	Watermelon (Charleston Gray)	lsiwcgB008
Lsi09G000540	Watermelon (Charleston Gray)	lsiwcgB018
Lsi09G000540	Watermelon (Charleston Gray)	lsiwcgB021
Lsi09G000540	Watermelon (97103) v1	lsiwmB018
Lsi09G000540	Watermelon (97103) v1	lsiwmB023
Lsi09G000540	Watermelon (97103) v1	lsiwmB027
Lsi09G000540	Cucumber (Gy14) v2	cgyblsiB137
Lsi09G000540	Cucumber (Gy14) v2	cgyblsiB294
Lsi09G000540	Melon (DHL92) v3.6.1	lsimedB012
Lsi09G000540	Melon (DHL92) v3.6.1	lsimedB033
Lsi09G000540	Melon (DHL92) v3.6.1	lsimedB039