Lsi08G014260 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi08G014260
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Allene oxide synthase
Location	chr08 : 22492550 .. 22494148 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGACTACCTCCATTATTATCCCTTCTCTTCAAACCCAGTTGCGATTCCCGTCGCAAGAAACGCCTCTGAGATCTCGTTCTAGAGTTGGGTTCGTTTCCCTACGCCCAATCTGCGCCGCCGACAGGGTTTCCTCCACGTCTTCCTCCTCTGTTCAAGTGCCGCAGCGGACTGTTTCGCCGCCGGAACCTACCAAGCTTCCTTTGAGGAAGATTCCAGGTGACTATGGGCCGCCGATGTTTGGGGCGTTGAAGGACCGACAAGACTATTTCTATAATCAGGGGAGGGAAGAGTATCTGAAATCTCGAATGCTTCGGTATGAATCCACTGTGTATAGAACCAATATGCCACCGGGTCCATTTATCACTTCCGATTCTCGAGTTGTTGTTTTACTCGACGGGAAGAGTTTTCCTGTTCTTTTCGACAATTCTAAAGTTGAGAAGAAGGATCTCTTTACCGGGACTTACATGCCTTTAACAGAGCTCACCGGCGGTTACAGGGTTCTCTCTTATATTGACCCATCTGAGCCCGATCACGAGAAGCTTAAACAGCTCATCTTCTTCCTCCTCAAGCACCGCCGGGATAAAATTTTGCCGGAATTTCACTCTACTTTCTCCGAGCTATTTGAGACTCTGGAGAAGGATTTGGCTGCTGCTGGTAAAGCCGAGTACAATGCTTCCGGTGAACAGGCGGCGTTTAATTTCTTGGCTCGGTCTCTCTTCGGAGCCAATCCGGTCGATACCAAATTGGGTCGTGACGCTCCGAAATTGATTTCGAAATGGGTTTTGTTCCAGCTTGGCCCTGTTCTCAGGCTCGGTCTCCCCAAGGTCGTCGAGGAGCTTCTCCTCCGCACGGTCCGGCTCCCGCCGGCGCTGATCAAAGCCGATTACCGGCGTCTGTATGACTTCTTTTACCAGTCGTCGGAGGCGGTGTTTGAGGAGGCGGATAGATTGGGAATTTCGAGGGAAGAAGCTTGCCACAACTTGCTATTCACAACTTGTTTTAATTCATTCGGTGGGATGAAGATCTTATTCCCCAATATGATCAAATGGATCGGCCGAGCCGGAGTAAATCTCCACACCCAACTCGCACGGGAGATTCGTACTGCCGTAAAAGCCAACGGCGGGAAAATCACGATGGGGGCTATGGAACAGATGCCGCTGATGAAATCAGTGGTATATGAAGCGTTCAGAATCGAGCCGCCGGTTCCGGTTCAGTACGGTCGGGCGAAGAAGGACCTTGTGGTCGAAAGCCACGACGCGGCTTTCGAGATCAAAGAAGGAGAAATGTTGTGTGGGTATCAGCCATTCGCGACCAGAGATCCAAAAATCTTCGACAGAGCCGACGAGTTCGTGCCTGATCGATTCACCGGCGACGGTGAAGAGTTGCTGAAACACGTGCTATGGTCAAACGGACCAGAGACCCAGTCGCCGACGGTTCAGAACAAGCAGTGCGCCGGAAAAGACTTCATCGTCTTCATCTCTCGCCTTCTCGTCGTCGAACTCTTCCTCCGGTACGACTCCTTCGACATCGAAGCCACAAACACTCCGTTAGGCGCCGCCGTCACTGTAACTTCCCTGAAGAAAGCAAGTTTCTGA

mRNA sequence

ATGTCGACTACCTCCATTATTATCCCTTCTCTTCAAACCCAGTTGCGATTCCCGTCGCAAGAAACGCCTCTGAGATCTCGTTCTAGAGTTGGGTTCGTTTCCCTACGCCCAATCTGCGCCGCCGACAGGGTTTCCTCCACGTCTTCCTCCTCTGTTCAAGTGCCGCAGCGGACTGTTTCGCCGCCGGAACCTACCAAGCTTCCTTTGAGGAAGATTCCAGGTGACTATGGGCCGCCGATGTTTGGGGCGTTGAAGGACCGACAAGACTATTTCTATAATCAGGGGAGGGAAGAGTATCTGAAATCTCGAATGCTTCGGTATGAATCCACTGTGTATAGAACCAATATGCCACCGGGTCCATTTATCACTTCCGATTCTCGAGTTGTTGTTTTACTCGACGGGAAGAGTTTTCCTGTTCTTTTCGACAATTCTAAAGTTGAGAAGAAGGATCTCTTTACCGGGACTTACATGCCTTTAACAGAGCTCACCGGCGGTTACAGGGTTCTCTCTTATATTGACCCATCTGAGCCCGATCACGAGAAGCTTAAACAGCTCATCTTCTTCCTCCTCAAGCACCGCCGGGATAAAATTTTGCCGGAATTTCACTCTACTTTCTCCGAGCTATTTGAGACTCTGGAGAAGGATTTGGCTGCTGCTGGTAAAGCCGAGTACAATGCTTCCGGTGAACAGGCGGCGTTTAATTTCTTGGCTCGGTCTCTCTTCGGAGCCAATCCGGTCGATACCAAATTGGGTCGTGACGCTCCGAAATTGATTTCGAAATGGGTTTTGTTCCAGCTTGGCCCTGTTCTCAGGCTCGGTCTCCCCAAGGTCGTCGAGGAGCTTCTCCTCCGCACGGTCCGGCTCCCGCCGGCGCTGATCAAAGCCGATTACCGGCGTCTGTATGACTTCTTTTACCAGTCGTCGGAGGCGGTGTTTGAGGAGGCGGATAGATTGGGAATTTCGAGGGAAGAAGCTTGCCACAACTTGCTATTCACAACTTGTTTTAATTCATTCGGTGGGATGAAGATCTTATTCCCCAATATGATCAAATGGATCGGCCGAGCCGGAGTAAATCTCCACACCCAACTCGCACGGGAGATTCGTACTGCCGTAAAAGCCAACGGCGGGAAAATCACGATGGGGGCTATGGAACAGATGCCGCTGATGAAATCAGTGGTATATGAAGCGTTCAGAATCGAGCCGCCGGTTCCGGTTCAGTACGGTCGGGCGAAGAAGGACCTTGTGGTCGAAAGCCACGACGCGGCTTTCGAGATCAAAGAAGGAGAAATGTTGTGTGGGTATCAGCCATTCGCGACCAGAGATCCAAAAATCTTCGACAGAGCCGACGAGTTCGTGCCTGATCGATTCACCGGCGACGGTGAAGAGTTGCTGAAACACGTGCTATGGTCAAACGGACCAGAGACCCAGTCGCCGACGGTTCAGAACAAGCAGTGCGCCGGAAAAGACTTCATCGTCTTCATCTCTCGCCTTCTCGTCGTCGAACTCTTCCTCCGGTACGACTCCTTCGACATCGAAGCCACAAACACTCCGTTAGGCGCCGCCGTCACTGTAACTTCCCTGAAGAAAGCAAGTTTCTGA

Coding sequence (CDS)

ATGTCGACTACCTCCATTATTATCCCTTCTCTTCAAACCCAGTTGCGATTCCCGTCGCAAGAAACGCCTCTGAGATCTCGTTCTAGAGTTGGGTTCGTTTCCCTACGCCCAATCTGCGCCGCCGACAGGGTTTCCTCCACGTCTTCCTCCTCTGTTCAAGTGCCGCAGCGGACTGTTTCGCCGCCGGAACCTACCAAGCTTCCTTTGAGGAAGATTCCAGGTGACTATGGGCCGCCGATGTTTGGGGCGTTGAAGGACCGACAAGACTATTTCTATAATCAGGGGAGGGAAGAGTATCTGAAATCTCGAATGCTTCGGTATGAATCCACTGTGTATAGAACCAATATGCCACCGGGTCCATTTATCACTTCCGATTCTCGAGTTGTTGTTTTACTCGACGGGAAGAGTTTTCCTGTTCTTTTCGACAATTCTAAAGTTGAGAAGAAGGATCTCTTTACCGGGACTTACATGCCTTTAACAGAGCTCACCGGCGGTTACAGGGTTCTCTCTTATATTGACCCATCTGAGCCCGATCACGAGAAGCTTAAACAGCTCATCTTCTTCCTCCTCAAGCACCGCCGGGATAAAATTTTGCCGGAATTTCACTCTACTTTCTCCGAGCTATTTGAGACTCTGGAGAAGGATTTGGCTGCTGCTGGTAAAGCCGAGTACAATGCTTCCGGTGAACAGGCGGCGTTTAATTTCTTGGCTCGGTCTCTCTTCGGAGCCAATCCGGTCGATACCAAATTGGGTCGTGACGCTCCGAAATTGATTTCGAAATGGGTTTTGTTCCAGCTTGGCCCTGTTCTCAGGCTCGGTCTCCCCAAGGTCGTCGAGGAGCTTCTCCTCCGCACGGTCCGGCTCCCGCCGGCGCTGATCAAAGCCGATTACCGGCGTCTGTATGACTTCTTTTACCAGTCGTCGGAGGCGGTGTTTGAGGAGGCGGATAGATTGGGAATTTCGAGGGAAGAAGCTTGCCACAACTTGCTATTCACAACTTGTTTTAATTCATTCGGTGGGATGAAGATCTTATTCCCCAATATGATCAAATGGATCGGCCGAGCCGGAGTAAATCTCCACACCCAACTCGCACGGGAGATTCGTACTGCCGTAAAAGCCAACGGCGGGAAAATCACGATGGGGGCTATGGAACAGATGCCGCTGATGAAATCAGTGGTATATGAAGCGTTCAGAATCGAGCCGCCGGTTCCGGTTCAGTACGGTCGGGCGAAGAAGGACCTTGTGGTCGAAAGCCACGACGCGGCTTTCGAGATCAAAGAAGGAGAAATGTTGTGTGGGTATCAGCCATTCGCGACCAGAGATCCAAAAATCTTCGACAGAGCCGACGAGTTCGTGCCTGATCGATTCACCGGCGACGGTGAAGAGTTGCTGAAACACGTGCTATGGTCAAACGGACCAGAGACCCAGTCGCCGACGGTTCAGAACAAGCAGTGCGCCGGAAAAGACTTCATCGTCTTCATCTCTCGCCTTCTCGTCGTCGAACTCTTCCTCCGGTACGACTCCTTCGACATCGAAGCCACAAACACTCCGTTAGGCGCCGCCGTCACTGTAACTTCCCTGAAGAAAGCAAGTTTCTGA

Protein sequence

MSTTSIIIPSLQTQLRFPSQETPLRSRSRVGFVSLRPICAADRVSSTSSSSVQVPQRTVSPPEPTKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFITSDSRVVVLLDGKSFPVLFDNSKVEKKDLFTGTYMPLTELTGGYRVLSYIDPSEPDHEKLKQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAAAGKAEYNASGEQAAFNFLARSLFGANPVDTKLGRDAPKLISKWVLFQLGPVLRLGLPKVVEELLLRTVRLPPALIKADYRRLYDFFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKILFPNMIKWIGRAGVNLHTQLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDAAFEIKEGEMLCGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPTVQNKQCAGKDFIVFISRLLVVELFLRYDSFDIEATNTPLGAAVTVTSLKKASF

Homology

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BLAST of Lsi08G014260 vs. Swiss-Prot
Match: AOS1_SOLLC (Allene oxide synthase 1, chloroplastic OS=Solanum lycopersicum GN=AOS1 PE=2 SV=1)

HSP 1 Score: 729.6 bits (1882), Expect = 2.5e-209
Identity = 354/536 (66.04%), Postives = 448/536 (83.58%), Query Frame = 1


Query: 1   MSTTSIIIPSLQTQLRFPSQETPLRSRSRVGF-VSLRPICAA-DRVSSTSSSSVQVPQRT 60
           M++TS+ +PSL+  L+FPS  +    ++   + VS+RPI A+   +    SS  Q P  +
Sbjct: 1   MASTSLSLPSLK--LQFPSHTSSSSRKNSSSYRVSIRPIQASVSEIPPYISSPSQSPSSS 60

Query: 61  VSPP-EPTKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMP 120
            SPP +  KLP +K+PGDYG P+ G  KDR DYFYNQG+ E+ KSR+ +++STV+RTNMP
Sbjct: 61  SSPPVKQAKLPAQKVPGDYGLPLVGPWKDRLDYFYNQGKNEFFKSRIQKHQSTVFRTNMP 120

Query: 121 PGPFITSDSRVVVLLDGKSFPVLFDNSKVEKKDLFTGTYMPLTELTGGYRVLSYIDPSEP 180
           PGPFI+ +  VVVLLDGKSFPVLFD SKVEKKDLFTGT+MP T+LTGGYRVLSY+DPSEP
Sbjct: 121 PGPFISFNPNVVVLLDGKSFPVLFDVSKVEKKDLFTGTFMPSTDLTGGYRVLSYLDPSEP 180

Query: 181 DHEKLKQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAAAGKAEYNASGEQAAFNFLA 240
           +H KLK+L+F+LL  RR++++PEFH+++SELFETLE +L+  GKA  NA+ +QAAFNFLA
Sbjct: 181 NHAKLKKLMFYLLSSRRNEVIPEFHNSYSELFETLENELSTKGKAGLNAANDQAAFNFLA 240

Query: 241 RSLFGANPVDTKLGRDAPKLISKWVLFQLGPVLRLGLPKVVEELLLRTVRLPPALIKADY 300
           RSL+G NP DT+LG D PKLI KWVLFQL P+L LGLPKV+E+L++ T RLPPAL+K DY
Sbjct: 241 RSLYGINPQDTELGTDGPKLIGKWVLFQLHPLLILGLPKVLEDLVMHTFRLPPALVKKDY 300

Query: 301 RRLYDFFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKILFPNMIKWIGRAGV 360
           +RLY+FFY++S +V +EA+++GISREEACHNLLF TCFNSFGG+KI FPNM+KWIGRAG 
Sbjct: 301 QRLYNFFYENSTSVLDEAEKIGISREEACHNLLFATCFNSFGGIKIFFPNMLKWIGRAGA 360

Query: 361 NLHTQLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVE 420
            LH+QLA+EIR+ + +N GK+TM AME+MPLMKSVVYE+ RIEPPV  QYGRAK D+V+E
Sbjct: 361 KLHSQLAQEIRSVISSNSGKVTMAAMEKMPLMKSVVYESLRIEPPVASQYGRAKHDMVIE 420

Query: 421 SHDAAFEIKEGEMLCGYQPFATRDPKIFDRADEFVPDRFTG-DGEELLKHVLWSNGPETQ 480
           SHDA+FEIKEGE+L GYQPFAT+DPKIFDR++EFV DRF G +GE+LLKHVLWSNG ET+
Sbjct: 421 SHDASFEIKEGELLYGYQPFATKDPKIFDRSEEFVADRFIGEEGEKLLKHVLWSNGSETE 480

Query: 481 SPTVQNKQCAGKDFIVFISRLLVVELFLRYDSFDIEATNTPLGAAVTVTSLKKASF 533
           + ++ NKQCAGKDF+V +SRLL+VELFLRYDSF+IE   +PLGAA+T+TSL++ASF
Sbjct: 481 NASINNKQCAGKDFVVLVSRLLLVELFLRYDSFEIEVGASPLGAAITLTSLRRASF 534

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BLAST of Lsi08G014260 vs. Swiss-Prot
Match: CP74_LINUS (Allene oxide synthase, chloroplastic OS=Linum usitatissimum GN=CYP74A PE=1 SV=1)

HSP 1 Score: 685.6 bits (1768), Expect = 4.2e-196
Identity = 325/503 (64.61%), Postives = 412/503 (81.91%), Query Frame = 1


Query: 34  SLRPICAADRVSSTSSSSVQVPQRTVSPP---EPTKLPLRKIPGDYGPPMFGALKDRQDY 93
           + RPI    + S    S +++P  T  PP   + T LP+R+IPGDYG P  G ++DR DY
Sbjct: 38  AFRPI----KASLFGDSPIKIPGITSQPPPSSDETTLPIRQIPGDYGLPGIGPIQDRLDY 97

Query: 94  FYNQGREEYLKSRMLRYESTVYRTNMPPGPFITSDSRVVVLLDGKSFPVLFDNSKVEKKD 153
           FYNQGREE+ KSR+ +Y+STVYR NMPPGPFI S+ RV+VLLD KSFPVLFD SKVEKKD
Sbjct: 98  FYNQGREEFFKSRLQKYKSTVYRANMPPGPFIASNPRVIVLLDAKSFPVLFDMSKVEKKD 157

Query: 154 LFTGTYMPLTELTGGYRVLSYIDPSEPDHEKLKQLIFFLLKHRRDKILPEFHSTFSELFE 213
           LFTGTYMP TELTGGYR+LSY+DPSEP+H KLKQL+F L+K+RRD ++PEF S+F++L E
Sbjct: 158 LFTGTYMPSTELTGGYRILSYLDPSEPNHTKLKQLLFNLIKNRRDYVIPEFSSSFTDLCE 217

Query: 214 TLEKDLAAAGKAEYNASGEQAAFNFLARSLFGANPVDTKLGRDAPKLISKWVLFQLGPVL 273
            +E DLA  GKA +N   EQAAFNFL+R+ FG  P+DT LG+DAP LISKWVLF L P+L
Sbjct: 218 VVEYDLATKGKAAFNDPAEQAAFNFLSRAFFGVKPIDTPLGKDAPSLISKWVLFNLAPIL 277

Query: 274 RLGLPKVVEELLLRTVRLPPALIKADYRRLYDFFYQSSEAVFEEADRLGISREEACHNLL 333
            +GLPK VEE  L +VRLPP L++ DY RLY+FF  ++ +V +EA++ GISR+EACHN+L
Sbjct: 278 SVGLPKEVEEATLHSVRLPPLLVQNDYHRLYEFFTSAAGSVLDEAEQSGISRDEACHNIL 337

Query: 334 FTTCFNSFGGMKILFPNMIKWIGRAGVNLHTQLAREIRTAVKA-NGGKITMGAMEQMPLM 393
           F  CFNS+GG KILFP+++KWIGRAG+ LHT+LA+EIR+A+++  GGK+TM AMEQMPLM
Sbjct: 338 FAVCFNSWGGFKILFPSLMKWIGRAGLELHTKLAQEIRSAIQSTGGGKVTMAAMEQMPLM 397

Query: 394 KSVVYEAFRIEPPVPVQYGRAKKDLVVESHDAAFEIKEGEMLCGYQPFATRDPKIFDRAD 453
           KSVVYE  RIEPPV +QYG+AKKD ++ESH+AA+++KEGEML GYQPFAT+DPKIFDR +
Sbjct: 398 KSVVYETLRIEPPVALQYGKAKKDFILESHEAAYQVKEGEMLFGYQPFATKDPKIFDRPE 457

Query: 454 EFVPDRFTGDGEELLKHVLWSNGPETQSPTVQNKQCAGKDFIVFISRLLVVELFLRYDSF 513
           EFV DRF G+G +L+++V+WSNGPET++P+V NKQCAGKDF+V  +RL VVELF RYDSF
Sbjct: 458 EFVADRFVGEGVKLMEYVMWSNGPETETPSVANKQCAGKDFVVMAARLFVVELFKRYDSF 517

Query: 514 DIEATNTPLGAAVTVTSLKKASF 533
           DIE   + LGA++T+TSLK+++F
Sbjct: 518 DIEVGTSSLGASITLTSLKRSTF 536

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BLAST of Lsi08G014260 vs. Swiss-Prot
Match: AOS2_SOLLC (Allene oxide synthase 2, chloroplastic OS=Solanum lycopersicum GN=AOS2 PE=1 SV=1)

HSP 1 Score: 684.9 bits (1766), Expect = 7.1e-196
Identity = 331/522 (63.41%), Postives = 414/522 (79.31%), Query Frame = 1


Query: 10  SLQTQLRFPSQETPLRSRSRVGFVSLRPICAADRVSSTSSSSVQVPQRTVSPPEPTKLPL 69
           +L   L  PS    + S+    + + RPI     VS +  S++++ Q       P KL  
Sbjct: 4   TLSFSLPLPSLHQKIPSK----YSTFRPII----VSLSDKSTIEITQ-------PIKLST 63

Query: 70  RKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFITSDSRVV 129
           R IPGDYG P  G  KDR DYFYNQG+ ++ +SR+ +Y+ST++RTNMPPGPFITS+ +V+
Sbjct: 64  RTIPGDYGLPGIGPWKDRLDYFYNQGKNDFFESRIAKYKSTIFRTNMPPGPFITSNPKVI 123

Query: 130 VLLDGKSFPVLFDNSKVEKKDLFTGTYMPLTELTGGYRVLSYIDPSEPDHEKLKQLIFFL 189
           VLLDGKSFPVLFD SKVEKKDLFTGT++P TELTGGYR+LSY+DPSEP+HEKLK+L+FFL
Sbjct: 124 VLLDGKSFPVLFDASKVEKKDLFTGTFVPSTELTGGYRILSYLDPSEPNHEKLKKLMFFL 183

Query: 190 LKHRRDKILPEFHSTFSELFETLEKDLAAAGKAEYNASGEQAAFNFLARSLFGANPVDTK 249
           L  RRD ++PEFH T++ELFETL+K++   G   +N+  +QAAFNFLARSLFG NPV+TK
Sbjct: 184 LSSRRDHVIPEFHETYTELFETLDKEMEEKGTVGFNSGSDQAAFNFLARSLFGVNPVETK 243

Query: 250 LGRDAPKLISKWVLFQLGPVLRLGLPKVVEELLLRTVRLPPALIKADYRRLYDFFYQSSE 309
           LG D P LI KW+L QL PV+ LGLPK ++++LL T RLPP L+K DY+RLYDFFY +S 
Sbjct: 244 LGTDGPALIGKWILLQLHPVITLGLPKFLDDVLLHTFRLPPILVKKDYQRLYDFFYTNSA 303

Query: 310 AVFEEADRLGISREEACHNLLFTTCFNSFGGMKILFPNMIKWIGRAGVNLHTQLAREIRT 369
            +F EA++LGIS++EACHNLLF TCFNSFGGMKI FPNM+K I +AGV +HT+LA EIR+
Sbjct: 304 NLFIEAEKLGISKDEACHNLLFATCFNSFGGMKIFFPNMLKSIAKAGVEIHTRLANEIRS 363

Query: 370 AVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDAAFEIKEGE 429
            VK+ GGKITM AME+MPLMKSVVYEA R++PPV  QYGRAK+DL +ESHDA FE+K+GE
Sbjct: 364 EVKSAGGKITMSAMEKMPLMKSVVYEALRVDPPVASQYGRAKQDLKIESHDAVFEVKKGE 423

Query: 430 MLCGYQPFATRDPKIFDRADEFVPDRFTG-DGEELLKHVLWSNGPETQSPTVQNKQCAGK 489
           +L GYQPFAT+DPKIFDR  EFV DRF G +GE+LLKHVLWSNGPET+SPTV NKQCAGK
Sbjct: 424 ILFGYQPFATKDPKIFDRPGEFVADRFVGEEGEKLLKHVLWSNGPETESPTVGNKQCAGK 483

Query: 490 DFIVFISRLLVVELFLRYDSFDIEATNTPLGAAVTVTSLKKA 531
           DF+V +SRL V E FLRY + +++   + LG+++T+TSLKKA
Sbjct: 484 DFVVMVSRLFVTEFFLRYGTLNVDVGTSALGSSITITSLKKA 510

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BLAST of Lsi08G014260 vs. Swiss-Prot
Match: CP74A_ARATH (Allene oxide synthase, chloroplastic OS=Arabidopsis thaliana GN=CYP74A PE=1 SV=3)

HSP 1 Score: 676.4 bits (1744), Expect = 2.5e-193
Identity = 323/486 (66.46%), Postives = 398/486 (81.89%), Query Frame = 1


Query: 49  SSSVQVPQRTVSPPEPTK-LPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRY 108
           +S  + P  TV+    +K LP+R IPG+YG P+ G +KDR DYFY+QG EE+ KSR+ +Y
Sbjct: 33  ASGSETPDLTVATRTGSKDLPIRNIPGNYGLPIVGPIKDRWDYFYDQGAEEFFKSRIRKY 92

Query: 109 ESTVYRTNMPPGPFITSDSRVVVLLDGKSFPVLFDNSKVEKKDLFTGTYMPLTELTGGYR 168
            STVYR NMPPG FI  + +VV LLDGKSFPVLFD  KVEKKDLFTGTYMP TELTGGYR
Sbjct: 93  NSTVYRVNMPPGAFIAENPQVVALLDGKSFPVLFDVDKVEKKDLFTGTYMPSTELTGGYR 152

Query: 169 VLSYIDPSEPDHEKLKQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAAAGKAEYNAS 228
           +LSY+DPSEP HEKLK L+FFLLK  R++I PEF +T+SELF++LEK+L+  GKA++  S
Sbjct: 153 ILSYLDPSEPKHEKLKNLLFFLLKSSRNRIFPEFQATYSELFDSLEKELSLKGKADFGGS 212

Query: 229 GEQAAFNFLARSLFGANPVDTKLGRDAPKLISKWVLFQLGPVLRLGLPKVVEELLLRTVR 288
            +  AFNFLAR+ +G NP DTKL  DAP LI+KWVLF L P+L +GLP+V+EE L+ T  
Sbjct: 213 SDGTAFNFLARAFYGTNPADTKLKADAPGLITKWVLFNLHPLLSIGLPRVIEEPLIHTFS 272

Query: 289 LPPALIKADYRRLYDFFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKILFPN 348
           LPPAL+K+DY+RLY+FF +S+  +  EAD+LGISREEA HNLLF TCFN++GGMKILFPN
Sbjct: 273 LPPALVKSDYQRLYEFFLESAGEILVEADKLGISREEATHNLLFATCFNTWGGMKILFPN 332

Query: 349 MIKWIGRAGVNLHTQLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQY 408
           M+K IGRAG  +H +LA EIR+ +K+NGG++TMGA+E+M L KSVVYE  R EPPV  QY
Sbjct: 333 MVKRIGRAGHQVHNRLAEEIRSVIKSNGGELTMGAIEKMELTKSVVYECLRFEPPVTAQY 392

Query: 409 GRAKKDLVVESHDAAFEIKEGEMLCGYQPFATRDPKIFDRADEFVPDRFTG-DGEELLKH 468
           GRAKKDLV+ESHDAAF++K GEML GYQP ATRDPKIFDRADEFVP+RF G +GE+LL+H
Sbjct: 393 GRAKKDLVIESHDAAFKVKAGEMLYGYQPLATRDPKIFDRADEFVPERFVGEEGEKLLRH 452

Query: 469 VLWSNGPETQSPTVQNKQCAGKDFIVFISRLLVVELFLRYDSFDIEATNTPLGAAVTVTS 528
           VLWSNGPET++PTV NKQCAGKDF+V ++RL V+E+F RYDSFDIE   +PLG++V  +S
Sbjct: 453 VLWSNGPETETPTVGNKQCAGKDFVVLVARLFVIEIFRRYDSFDIEVGTSPLGSSVNFSS 512

Query: 529 LKKASF 533
           L+KASF
Sbjct: 513 LRKASF 518

[Close]

BLAST of Lsi08G014260 vs. Swiss-Prot
Match: C74A2_PARAR (Allene oxide synthase OS=Parthenium argentatum GN=CYP74A2 PE=1 SV=2)

HSP 1 Score: 643.3 bits (1658), Expect = 2.4e-183
Identity = 304/471 (64.54%), Postives = 380/471 (80.68%), Query Frame = 1


Query: 63  EPTKLPLRKIPGDYGPPMFGALKDRQDYFYNQG-REEYLKSRMLRYESTVYRTNMPPGPF 122
           +P+  PLR+IPG YG P F  +KDR +YFY  G R+EY +SRM +Y+STV+R NMPPGPF
Sbjct: 2   DPSSKPLREIPGSYGIPFFQPIKDRLEYFYGTGGRDEYFRSRMQKYQSTVFRANMPPGPF 61

Query: 123 ITSDSRVVVLLDGKSFPVLFDNSKVEKKDLFTGTYMPLTELTGGYRVLSYIDPSEPDHEK 182
           ++S+ +V+VLLD KSFP+LFD SKVEKKDLFTGTYMP T+LTGGYRVLSY+DPSEP H +
Sbjct: 62  VSSNPKVIVLLDAKSFPILFDVSKVEKKDLFTGTYMPSTKLTGGYRVLSYLDPSEPRHAQ 121

Query: 183 LKQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAAAGKAEYNASGEQAAFNFLARSLF 242
           LK L+FF+LK+  ++++P+F +T++ELFE LE +LA  GKA +N  GEQAAF FL R+ F
Sbjct: 122 LKNLLFFMLKNSSNRVIPQFETTYTELFEGLEAELAKNGKAAFNDVGEQAAFRFLGRAYF 181

Query: 243 GANPVDTKLGRDAPKLISKWVLFQLGPVLRLGLPKVVEELLLRTVRLPPALIKADYRRLY 302
            +NP +TKLG  AP LIS WVLF L P L LGLP  ++E LL T RLP  LIK+ Y +LY
Sbjct: 182 NSNPEETKLGTSAPTLISSWVLFNLAPTLDLGLPWFLQEPLLHTFRLPAFLIKSTYNKLY 241

Query: 303 DFFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKILFPNMIKWIGRAGVNLHT 362
           D+F   +  V E+A++LG+ ++EA HN+LF  CFN+FGG+KILFPN +KWIG AG NLHT
Sbjct: 242 DYFQSVATPVMEQAEKLGVPKDEAVHNILFAVCFNTFGGVKILFPNTLKWIGLAGENLHT 301

Query: 363 QLAREIRTAVKANG-GKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHD 422
           QLA EIR A+K+ G G +T+ A+EQMPL KSVVYE+ RIEPPVP QYG+AK +  +ESHD
Sbjct: 302 QLAEEIRGAIKSYGDGNVTLEAIEQMPLTKSVVYESLRIEPPVPPQYGKAKSNFTIESHD 361

Query: 423 AAFEIKEGEMLCGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPTV 482
           A FE+K+GEML GYQPFAT+DPK+FDR +E+VPDRF GDGE LLK+V WSNGPET+SPTV
Sbjct: 362 ATFEVKKGEMLFGYQPFATKDPKVFDRPEEYVPDRFVGDGEALLKYVWWSNGPETESPTV 421

Query: 483 QNKQCAGKDFIVFISRLLVVELFLRYDSFDIEATNTPLGAAVTVTSLKKAS 532
           +NKQCAGKDF+V I+RL V+ELF RYDSF+IE   +PLGAAVT+T LK+AS
Sbjct: 422 ENKQCAGKDFVVLITRLFVIELFRRYDSFEIELGESPLGAAVTLTFLKRAS 472

[Close]

BLAST of Lsi08G014260 vs. TrEMBL
Match: U5YTX9_CUCSA (Allene oxide synthase OS=Cucumis sativus GN=AOS PE=2 SV=1)

HSP 1 Score: 995.3 bits (2572), Expect = 2.8e-287
Identity = 497/531 (93.60%), Postives = 518/531 (97.55%), Query Frame = 1


Query: 4   TSIIIPSLQTQLRFPS-QETPLRSRSRVGFVSLRPIC-AADRVSSTSSSSVQVPQRTVSP 63
           +SI+IPSLQ  LRFPS QETP RSRSRVGFVS+RPI  AAD VSS+SSSS++VPQR VSP
Sbjct: 2   SSIVIPSLQPHLRFPSSQETPQRSRSRVGFVSIRPIVYAADGVSSSSSSSLRVPQRIVSP 61

Query: 64  PEPTKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPF 123
           PEP KLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPF
Sbjct: 62  PEPIKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPF 121

Query: 124 ITSDSRVVVLLDGKSFPVLFDNSKVEKKDLFTGTYMPLTELTGGYRVLSYIDPSEPDHEK 183
           ITSDSRVVVLLDGKSFPVLFD+SKVEKKDLFTGTYMP+TELTGGYRVLSYIDPSEPDH K
Sbjct: 122 ITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAK 181

Query: 184 LKQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAAAGKAEYNASGEQAAFNFLARSLF 243
           LKQL+FFLLKHRRDKI+PEFHSTFSELFETLEKDLAA+G+AEYNA GEQAAFNFLARSLF
Sbjct: 182 LKQLVFFLLKHRRDKIMPEFHSTFSELFETLEKDLAASGRAEYNAPGEQAAFNFLARSLF 241

Query: 244 GANPVDTKLGRDAPKLISKWVLFQLGPVLRLGLPKVVEELLLRTVRLPPALIKADYRRLY 303
           GA+PVD+KLGRDAPKLI+KWVLFQLGPVL LGLPKVVEELLLRTVRLPPALIKADYRRLY
Sbjct: 242 GADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLY 301

Query: 304 DFFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKILFPNMIKWIGRAGVNLHT 363
           +FFY+SSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKI FPNMIKWIGRAGVNLHT
Sbjct: 302 EFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHT 361

Query: 364 QLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDA 423
           QLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDA
Sbjct: 362 QLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDA 421

Query: 424 AFEIKEGEMLCGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPTVQ 483
           AFEIKEGEM+CGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSP+VQ
Sbjct: 422 AFEIKEGEMICGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPSVQ 481

Query: 484 NKQCAGKDFIVFISRLLVVELFLRYDSFDIEATNTPLGAAVTVTSLKKASF 533
           NKQCAGKDFIVFISRL+VVELFLRYDSFDIEA+NTPLGAAVTVTSLKKASF
Sbjct: 482 NKQCAGKDFIVFISRLMVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 532

[Close]

BLAST of Lsi08G014260 vs. TrEMBL
Match: Q8L5M5_CUCME (Allene oxide synthase OS=Cucumis melo GN=AOS PE=2 SV=1)

HSP 1 Score: 988.8 bits (2555), Expect = 2.6e-285
Identity = 495/536 (92.35%), Postives = 516/536 (96.27%), Query Frame = 1


Query: 4   TSIIIPSLQTQLRFPS-QETPLRSRSRVGFVSLRPICAADRVSSTSSSSVQVPQRTVSPP 63
           +SI+IPSLQ  LRFPS QETP RSRSRVGFVS+RPI A D VSS+SSSS+QVPQR VSPP
Sbjct: 2   SSIVIPSLQPHLRFPSSQETPQRSRSRVGFVSIRPIYATDGVSSSSSSSLQVPQRIVSPP 61

Query: 64  EPTKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFI 123
           EPTKLPLRK+PGDYGPPMFGALKDR DYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFI
Sbjct: 62  EPTKLPLRKVPGDYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFI 121

Query: 124 TSDSRVVVLLDGKSFPVLFDNSKVEKKDLFTGTYMPLTELTGGYRVLSYIDPSEPDHEKL 183
           TSDSRVVVLLDGKSFPVLFD+SKVEKKDLFTGTYMP+TELTGGYRVLSYIDPSEPDH KL
Sbjct: 122 TSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAKL 181

Query: 184 KQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAAAGKAEYNASGEQAAFNFLARSLFG 243
           KQLIFFLLKHRRDKI+PEFHSTFSELFETLEKDLAAAG+AEYNASGEQAAFNFLARSLFG
Sbjct: 182 KQLIFFLLKHRRDKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLARSLFG 241

Query: 244 ANPVDTKLGRDAPKLISKWVLFQLGPVLRLGLPKVVEELLLRTVRLPPALIKADYRRLYD 303
           A+PVD+KLGRDAPKLI+KWVLFQLGPVL LGLPKVVEELLLRTVRLPPALIKADYRRLYD
Sbjct: 242 ADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLYD 301

Query: 304 FFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKILFPNMIKWIGRAGVNLHTQ 363
           FFY+SSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKI FPNMIKWIGRAGVNLHT+
Sbjct: 302 FFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHTR 361

Query: 364 LAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDAA 423
           LAREIRTAVKANGGKITMGAMEQMPLMKSVVYEA RIEPPVPVQYGRAKKDLVVESHDAA
Sbjct: 362 LAREIRTAVKANGGKITMGAMEQMPLMKSVVYEALRIEPPVPVQYGRAKKDLVVESHDAA 421

Query: 424 FEIKEGEMLCGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPTVQN 483
           FEIKEGE++CGYQPFATRDPKIFDRADE VPDRFTG+GEELLKHV+WSNGPETQSP+VQN
Sbjct: 422 FEIKEGEVICGYQPFATRDPKIFDRADELVPDRFTGEGEELLKHVIWSNGPETQSPSVQN 481

Query: 484 KQCAGKDFIVFISRLLVVELFLRYDSFDIEATNTPLG------AAVTVTSLKKASF 533
           KQCAGKDFIVFISRLLVVELFLRYDSFDIEA+NTPLG      AAVTVTSLKKASF
Sbjct: 482 KQCAGKDFIVFISRLLVVELFLRYDSFDIEASNTPLGAAVTVSAAVTVTSLKKASF 537

[Close]

BLAST of Lsi08G014260 vs. TrEMBL
Match: A0A023UKW8_CAMSI (Allene oxide synthase OS=Camellia sinensis GN=AOS2 PE=2 SV=1)

HSP 1 Score: 760.0 bits (1961), Expect = 1.9e-216
Identity = 373/534 (69.85%), Postives = 448/534 (83.90%), Query Frame = 1


Query: 1   MSTTSIIIPSLQTQLRFPSQETPLRSRSRVGFVSLRPICAADRVSSTSSSSVQVPQRTVS 60
           M+++S+  PSLQ  L+FP+Q +   S  R+    +RPI A     S S      P   V 
Sbjct: 1   MASSSLTFPSLQ--LQFPTQRSSKPSSRRL---IVRPITA-----SISEKPSATPTPAVK 60

Query: 61  PPE-PTKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPG 120
           PP+ PT LP+RKIPG+YG P+ G LKDR DYFYNQGR+EY KSR+ +Y+STV+R NMPPG
Sbjct: 61  PPQPPTNLPIRKIPGNYGIPLIGPLKDRLDYFYNQGRDEYFKSRVQKYQSTVFRANMPPG 120

Query: 121 PFITSDSRVVVLLDGKSFPVLFDNSKVEKKDLFTGTYMPLTELTGGYRVLSYIDPSEPDH 180
           PFI S+  VVVLLDGKSFP+LFD +KV+K+D+FTGTYMP TELTGGYRVLSY+DPSEP+H
Sbjct: 121 PFIASNPNVVVLLDGKSFPILFDVTKVKKRDVFTGTYMPSTELTGGYRVLSYLDPSEPNH 180

Query: 181 EKLKQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAAAGKAEYNASGEQAAFNFLARS 240
            KLKQL+FFLLK  RD+++PEF ++F+ELFE LE +L + GKA +N + +QAAFNFLARS
Sbjct: 181 AKLKQLMFFLLKSSRDRVIPEFQTSFTELFEMLESELGSEGKASFNEANDQAAFNFLARS 240

Query: 241 LFGANPVDTKLGRDAPKLISKWVLFQLGPVLRLGLPKVVEELLLRTVRLPPALIKADYRR 300
           L+G NPVDTKLG D PKL++KWV+FQLGP+L LGLPK VEEL++ T RLPPAL+K DY+R
Sbjct: 241 LYGTNPVDTKLGIDGPKLVTKWVVFQLGPLLILGLPKFVEELVIHTFRLPPALVKKDYQR 300

Query: 301 LYDFFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKILFPNMIKWIGRAGVNL 360
           LYDFFY SS  V +EA+R+GISREEACHNL+F TCFNSFGGMKILFPNMIK IGRAGVNL
Sbjct: 301 LYDFFYDSSTLVLDEAERIGISREEACHNLIFATCFNSFGGMKILFPNMIKRIGRAGVNL 360

Query: 361 HTQLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESH 420
           HTQLA EIR+AV++NGG++TM AMEQMPLMKSVVYE+ RIEPP+ +QYGRAK+DLV+ESH
Sbjct: 361 HTQLAEEIRSAVRSNGGEVTMAAMEQMPLMKSVVYESLRIEPPISLQYGRAKRDLVIESH 420

Query: 421 DAAFEIKEGEMLCGYQPFATRDPKIFDRADEFVPDRFTG-DGEELLKHVLWSNGPETQSP 480
           DAAFE+KEGE+L G+QPFAT+DPKIF+R +EFV DRF G +GE LL+HVLWSNGPET+SP
Sbjct: 421 DAAFEVKEGELLFGFQPFATKDPKIFERPEEFVADRFVGEEGERLLRHVLWSNGPETESP 480

Query: 481 TVQNKQCAGKDFIVFISRLLVVELFLRYDSFDIEATNTPLGAAVTVTSLKKASF 533
           TV NKQCAGKDF+VF+SRLLVVELF RYDSF+IE   +PLGA VTVTSLK+ASF
Sbjct: 481 TVGNKQCAGKDFVVFVSRLLVVELFRRYDSFEIEVGQSPLGAKVTVTSLKRASF 524

[Close]

BLAST of Lsi08G014260 vs. TrEMBL
Match: W9S158_9ROSA (Allene oxide synthase OS=Morus notabilis GN=L484_021251 PE=3 SV=1)

HSP 1 Score: 756.1 bits (1951), Expect = 2.8e-215
Identity = 376/533 (70.54%), Postives = 444/533 (83.30%), Query Frame = 1


Query: 1   MSTTSIIIPSLQTQLRFPSQETPLRSRSRVGFVSLRPICAADRVSSTSSSSVQVPQRTVS 60
           M++TS+  PSLQ Q R  S+++  RS  R  FVS  PI A    S +  SSV VP   V 
Sbjct: 1   MASTSLAFPSLQPQFR-SSRKSHKRSNRR--FVSF-PIHA----SVSEKSSVPVPPAAVE 60

Query: 61  PPEPTKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGP 120
             EP+KLP+RKIPG YG P  G + DR DYFYNQGR+EY KSR+ +Y+STV+R N+PPGP
Sbjct: 61  QHEPSKLPIRKIPGKYGLPFIGPVADRLDYFYNQGRDEYFKSRIQKYQSTVFRANIPPGP 120

Query: 121 FITSDSRVVVLLDGKSFPVLFDNSKVEKKDLFTGTYMPLTELTGGYRVLSYIDPSEPDHE 180
            ITS+ RV+VLLDGKSFPVLFD +KVEKKDLFTGT+MP T+LTGGYRVLSY+DPSEP+H 
Sbjct: 121 LITSNPRVIVLLDGKSFPVLFDVTKVEKKDLFTGTFMPSTDLTGGYRVLSYLDPSEPNHG 180

Query: 181 KLKQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAAAGKAEYNASGEQAAFNFLARSL 240
           K+K LIFFLLK RRDK++PEFHS+++ELFETLE +LAA GK+ +  + +QAAFNFL+R+L
Sbjct: 181 KIKSLIFFLLKSRRDKVIPEFHSSYTELFETLESELAAKGKSNFGEANDQAAFNFLSRAL 240

Query: 241 FGANPVDTKLGRDAPKLISKWVLFQLGPVLRLGLPKVVEELLLRTVRLPPALIKADYRRL 300
           +G NP DTKLG D P L+ KWV+FQL P+L LGLPK VEEL L + RLPP L+K+DY+RL
Sbjct: 241 YGTNPADTKLGNDGPGLVQKWVVFQLSPILVLGLPKFVEELTLHSFRLPPFLVKSDYQRL 300

Query: 301 YDFFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKILFPNMIKWIGRAGVNLH 360
           YDFFY+SS  V +EA+RLGISREEACHNLLF TCFNSFGGMKILFPNM+K IGRAGV LH
Sbjct: 301 YDFFYESSGHVLDEAERLGISREEACHNLLFATCFNSFGGMKILFPNMLKQIGRAGVKLH 360

Query: 361 TQLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHD 420
           T+LAREIRT V++NGGK+TM AMEQMPLMKSVVYE+ RIEPPV +Q+G+AK+D V+ESHD
Sbjct: 361 TELAREIRTVVRSNGGKVTMAAMEQMPLMKSVVYESLRIEPPVSLQFGKAKRDFVIESHD 420

Query: 421 AAFEIKEGEMLCGYQPFATRDPKIFDRADEFVPDRFTG-DGEELLKHVLWSNGPETQSPT 480
           AAFEIKEGEM+ GYQPFAT+DPKIFDRA+EFVPDRF G +GE LLKHVLWSNGPET+SPT
Sbjct: 421 AAFEIKEGEMIFGYQPFATKDPKIFDRAEEFVPDRFVGEEGERLLKHVLWSNGPETESPT 480

Query: 481 VQNKQCAGKDFIVFISRLLVVELFLRYDSFDIEATNTPLGAAVTVTSLKKASF 533
           V NKQCAGKD +V  SRLLVVELFL YDSF+IE   + LG+AVTVTSLK+ASF
Sbjct: 481 VGNKQCAGKDLVVLFSRLLVVELFLLYDSFEIEVGTSALGSAVTVTSLKRASF 525

[Close]

BLAST of Lsi08G014260 vs. TrEMBL
Match: A0A0U5B3Q9_CAMSI (CYP74A52 OS=Camellia sinensis GN=cytochrome P450 PE=2 SV=1)

HSP 1 Score: 753.4 bits (1944), Expect = 1.8e-214
Identity = 370/529 (69.94%), Postives = 443/529 (83.74%), Query Frame = 1


Query: 1   MSTTSIIIPSLQTQLRFPSQETPLRSRSRVGFVSLRPICAADRVSSTSSSSVQVPQRTVS 60
           M+++S+  PSLQ  L+FP+Q +   S  R+    +RPI A     S S      P   V 
Sbjct: 1   MASSSLTFPSLQ--LQFPTQRSSKPSSRRL---IVRPITA-----SISEKPSATPTPAVK 60

Query: 61  PPE-PTKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPG 120
           PP+ PT LP+RKIPG+YG P+ G LKDR DYFYNQGR+EY KSR+ +Y+STV+R NMPPG
Sbjct: 61  PPQPPTNLPIRKIPGNYGIPLIGPLKDRLDYFYNQGRDEYFKSRVQKYQSTVFRANMPPG 120

Query: 121 PFITSDSRVVVLLDGKSFPVLFDNSKVEKKDLFTGTYMPLTELTGGYRVLSYIDPSEPDH 180
           PFI S+  VVVLLDGKSFP+LFD +KVEK+D+FTGTYMP TELTGGYRVLSY+DPSEP+H
Sbjct: 121 PFIASNPNVVVLLDGKSFPILFDVTKVEKRDVFTGTYMPSTELTGGYRVLSYLDPSEPNH 180

Query: 181 EKLKQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAAAGKAEYNASGEQAAFNFLARS 240
            KLKQL+FFLLK  RD+++PEF ++F+ELFE LE +L + GKA +N + +QAAFNFLARS
Sbjct: 181 AKLKQLMFFLLKSSRDRVIPEFQTSFTELFEMLESELGSEGKASFNEANDQAAFNFLARS 240

Query: 241 LFGANPVDTKLGRDAPKLISKWVLFQLGPVLRLGLPKVVEELLLRTVRLPPALIKADYRR 300
           L+G NPVDTKLG D PKL++KWV+FQLGP+L LGLPK VEEL++ T RLPPAL+K DY+R
Sbjct: 241 LYGTNPVDTKLGIDGPKLVTKWVVFQLGPLLILGLPKFVEELVIHTFRLPPALVKKDYQR 300

Query: 301 LYDFFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKILFPNMIKWIGRAGVNL 360
           LYDFFY SS  V +EA+R+GISREEACHNL+F TCFNSFGGMKILFPNMIK IGRAGVNL
Sbjct: 301 LYDFFYDSSTLVLDEAERIGISREEACHNLIFATCFNSFGGMKILFPNMIKRIGRAGVNL 360

Query: 361 HTQLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESH 420
           HTQLA EIR+AV++NGG++TM AMEQMPLMKSVVYE+ RIEPP+ +QYGRAK+DLV+ESH
Sbjct: 361 HTQLAEEIRSAVRSNGGEVTMAAMEQMPLMKSVVYESLRIEPPISLQYGRAKRDLVIESH 420

Query: 421 DAAFEIKEGEMLCGYQPFATRDPKIFDRADEFVPDRFTG-DGEELLKHVLWSNGPETQSP 480
           DAAFE+KEGE+L G+QPFAT+DPKIF+R +EFV DRF G +GE LL+HVLWSNGPET+SP
Sbjct: 421 DAAFEVKEGELLFGFQPFATKDPKIFERPEEFVADRFVGEEGERLLRHVLWSNGPETESP 480

Query: 481 TVQNKQCAGKDFIVFISRLLVVELFLRYDSFDIEATNTPLGAAVTVTSL 528
           TV NKQCAGKDF+VF+SRLLVVELF RYDSF+IE   +PLGA VTVTSL
Sbjct: 481 TVGNKQCAGKDFVVFVSRLLVVELFRRYDSFEIEVGQSPLGAKVTVTSL 519

[Close]

BLAST of Lsi08G014260 vs. TAIR10
Match: AT5G42650.1 (AT5G42650.1 allene oxide synthase)

HSP 1 Score: 676.4 bits (1744), Expect = 1.4e-194
Identity = 323/486 (66.46%), Postives = 398/486 (81.89%), Query Frame = 1


Query: 49  SSSVQVPQRTVSPPEPTK-LPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRY 108
           +S  + P  TV+    +K LP+R IPG+YG P+ G +KDR DYFY+QG EE+ KSR+ +Y
Sbjct: 33  ASGSETPDLTVATRTGSKDLPIRNIPGNYGLPIVGPIKDRWDYFYDQGAEEFFKSRIRKY 92

Query: 109 ESTVYRTNMPPGPFITSDSRVVVLLDGKSFPVLFDNSKVEKKDLFTGTYMPLTELTGGYR 168
            STVYR NMPPG FI  + +VV LLDGKSFPVLFD  KVEKKDLFTGTYMP TELTGGYR
Sbjct: 93  NSTVYRVNMPPGAFIAENPQVVALLDGKSFPVLFDVDKVEKKDLFTGTYMPSTELTGGYR 152

Query: 169 VLSYIDPSEPDHEKLKQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAAAGKAEYNAS 228
           +LSY+DPSEP HEKLK L+FFLLK  R++I PEF +T+SELF++LEK+L+  GKA++  S
Sbjct: 153 ILSYLDPSEPKHEKLKNLLFFLLKSSRNRIFPEFQATYSELFDSLEKELSLKGKADFGGS 212

Query: 229 GEQAAFNFLARSLFGANPVDTKLGRDAPKLISKWVLFQLGPVLRLGLPKVVEELLLRTVR 288
            +  AFNFLAR+ +G NP DTKL  DAP LI+KWVLF L P+L +GLP+V+EE L+ T  
Sbjct: 213 SDGTAFNFLARAFYGTNPADTKLKADAPGLITKWVLFNLHPLLSIGLPRVIEEPLIHTFS 272

Query: 289 LPPALIKADYRRLYDFFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKILFPN 348
           LPPAL+K+DY+RLY+FF +S+  +  EAD+LGISREEA HNLLF TCFN++GGMKILFPN
Sbjct: 273 LPPALVKSDYQRLYEFFLESAGEILVEADKLGISREEATHNLLFATCFNTWGGMKILFPN 332

Query: 349 MIKWIGRAGVNLHTQLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQY 408
           M+K IGRAG  +H +LA EIR+ +K+NGG++TMGA+E+M L KSVVYE  R EPPV  QY
Sbjct: 333 MVKRIGRAGHQVHNRLAEEIRSVIKSNGGELTMGAIEKMELTKSVVYECLRFEPPVTAQY 392

Query: 409 GRAKKDLVVESHDAAFEIKEGEMLCGYQPFATRDPKIFDRADEFVPDRFTG-DGEELLKH 468
           GRAKKDLV+ESHDAAF++K GEML GYQP ATRDPKIFDRADEFVP+RF G +GE+LL+H
Sbjct: 393 GRAKKDLVIESHDAAFKVKAGEMLYGYQPLATRDPKIFDRADEFVPERFVGEEGEKLLRH 452

Query: 469 VLWSNGPETQSPTVQNKQCAGKDFIVFISRLLVVELFLRYDSFDIEATNTPLGAAVTVTS 528
           VLWSNGPET++PTV NKQCAGKDF+V ++RL V+E+F RYDSFDIE   +PLG++V  +S
Sbjct: 453 VLWSNGPETETPTVGNKQCAGKDFVVLVARLFVIEIFRRYDSFDIEVGTSPLGSSVNFSS 512

Query: 529 LKKASF 533
           L+KASF
Sbjct: 513 LRKASF 518

[Close]

BLAST of Lsi08G014260 vs. TAIR10
Match: AT4G15440.1 (AT4G15440.1 hydroperoxide lyase 1)

HSP 1 Score: 278.1 bits (710), Expect = 1.1e-74
Identity = 143/367 (38.96%), Postives = 216/367 (58.86%), Query Frame = 1


Query: 146 VEKKDLFTGTYMPLTELTGGYRVLSYIDPSEPDHEKLKQLIFFLLKHRRDKILPEFHSTF 205
           V+K+D+  G + P     GG RV  Y+D +EP H K+K      LK      L E  S  
Sbjct: 4   VDKRDVLIGDFRPSLGFYGGVRVGVYLDTTEPKHAKIKGFAMETLKRSSKVWLQELRSNL 63

Query: 206 SELFETLEKDLAAAGKAEYNASGEQAAFNFLARSLFGANP-VDTKLGRDAPKLISKWVLF 265
           +  + T+E +++  G A Y    ++  F+FL  SL G +  V   +  +  K I+ W+  
Sbjct: 64  NIFWGTIESEISKNGAASYIFPLQRCIFSFLCASLAGVDASVSPDIAENGWKTINTWLAL 123

Query: 266 QLGPVLRLGL-PKVVEELLLRTVRLPPALIKADYRRLYDFFYQSS-EAVFEEADRLGISR 325
           Q+ P  +LG+ P+ +EE+LL T   P  LI  +Y++LY+F  +++ + +    +  G++R
Sbjct: 124 QVIPTAKLGVVPQPLEEILLHTWPYPSLLIAGNYKKLYNFIDENAGDCLRLGQEEFGLTR 183

Query: 326 EEACHNLLFTTCFNSFGGMKILFPNMIKWIGRAGVNLHTQLAREIRTAVKANGGKITMGA 385
           +EA  NLLF   FN++GG  +  P++I  I      L  ++  E+R  V  +G  +    
Sbjct: 184 DEAIQNLLFVLGFNAYGGFSVFLPSLIGRITGDNSGLQERIRTEVRR-VCGSGSDLNFKT 243

Query: 386 MEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDAAFEIKEGEMLCGYQPFATRDP 445
           + +M L+KSVVYE  R  PPVP+Q+ RA+KD  + SHDA FE+K+GE+LCGYQP   RD 
Sbjct: 244 VNEMELVKSVVYETLRFSPPVPLQFARARKDFQISSHDAVFEVKKGELLCGYQPLVMRDA 303

Query: 446 KIFDRADEFVPDRFTGD-GEELLKHVLWSNGPETQSPTVQNKQCAGKDFIVFISRLLVVE 505
            +FD  +EF PDR+ G+ G ELL ++ WSNGP+T +P+  NKQCA KD +   + LLV +
Sbjct: 304 NVFDEPEEFKPDRYVGETGSELLNYLYWSNGPQTGTPSASNKQCAAKDIVTLTASLLVAD 363

Query: 506 LFLRYDS 509
           LFLRYD+
Sbjct: 364 LFLRYDT 369

[Close]

BLAST of Lsi08G014260 vs. NCBI nr
Match: gi|659087845|ref|XP_008444665.1| (PREDICTED: allene oxide synthase [Cucumis melo])

HSP 1 Score: 997.7 bits (2578), Expect = 8.0e-288
Identity = 496/530 (93.58%), Postives = 517/530 (97.55%), Query Frame = 1


Query: 4   TSIIIPSLQTQLRFPS-QETPLRSRSRVGFVSLRPICAADRVSSTSSSSVQVPQRTVSPP 63
           +SI+IPSLQ  LRFPS QETP RSRSRVGFVS+RPI A D VSS+SSSS+QVPQR VSPP
Sbjct: 2   SSIVIPSLQPHLRFPSSQETPQRSRSRVGFVSIRPIYATDGVSSSSSSSLQVPQRIVSPP 61

Query: 64  EPTKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFI 123
           EPTKLPLRK+PGDYGPPMFGALKDR DYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFI
Sbjct: 62  EPTKLPLRKVPGDYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFI 121

Query: 124 TSDSRVVVLLDGKSFPVLFDNSKVEKKDLFTGTYMPLTELTGGYRVLSYIDPSEPDHEKL 183
           TSDSRVVVLLDGKSFPVLFD+SKVEKKDLFTGTYMP+TELTGGYRVLSYIDPSEPDH KL
Sbjct: 122 TSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAKL 181

Query: 184 KQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAAAGKAEYNASGEQAAFNFLARSLFG 243
           KQLIFFLLKHRRDKI+PEFHSTFSELFETLEKDLAAAG+AEYNASGEQAAFNFLARSLFG
Sbjct: 182 KQLIFFLLKHRRDKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLARSLFG 241

Query: 244 ANPVDTKLGRDAPKLISKWVLFQLGPVLRLGLPKVVEELLLRTVRLPPALIKADYRRLYD 303
           A+PVD+KLGRDAPKLI+KWVLFQLGPVL LGLPKVVEELLLRTVRLPPALIKADYRRLYD
Sbjct: 242 ADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLYD 301

Query: 304 FFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKILFPNMIKWIGRAGVNLHTQ 363
           FFY+SSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKI FPNMIKWIGRAGVNLHT+
Sbjct: 302 FFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHTR 361

Query: 364 LAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDAA 423
           LAREIRTAVKANGGKITMGAMEQMPLMKSVVYEA RIEPPVPVQYGRAKKDLVVESHDAA
Sbjct: 362 LAREIRTAVKANGGKITMGAMEQMPLMKSVVYEALRIEPPVPVQYGRAKKDLVVESHDAA 421

Query: 424 FEIKEGEMLCGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPTVQN 483
           FEIKEGE++CGYQPFATRDPKIFDRADEFVPDRFTG+GEELLKHV+WSNGPETQSP+VQN
Sbjct: 422 FEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTGEGEELLKHVIWSNGPETQSPSVQN 481

Query: 484 KQCAGKDFIVFISRLLVVELFLRYDSFDIEATNTPLGAAVTVTSLKKASF 533
           KQCAGKDFIVFISRLLVVELFLRYDSFDIEA+NTPLGAAVTVTSLKKASF
Sbjct: 482 KQCAGKDFIVFISRLLVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 531

[Close]

BLAST of Lsi08G014260 vs. NCBI nr
Match: gi|566006191|ref|NP_001274390.1| (allene oxide synthase [Cucumis sativus])

HSP 1 Score: 995.3 bits (2572), Expect = 3.9e-287
Identity = 497/531 (93.60%), Postives = 518/531 (97.55%), Query Frame = 1


Query: 4   TSIIIPSLQTQLRFPS-QETPLRSRSRVGFVSLRPIC-AADRVSSTSSSSVQVPQRTVSP 63
           +SI+IPSLQ  LRFPS QETP RSRSRVGFVS+RPI  AAD VSS+SSSS++VPQR VSP
Sbjct: 2   SSIVIPSLQPHLRFPSSQETPQRSRSRVGFVSIRPIVYAADGVSSSSSSSLRVPQRIVSP 61

Query: 64  PEPTKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPF 123
           PEP KLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPF
Sbjct: 62  PEPIKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPF 121

Query: 124 ITSDSRVVVLLDGKSFPVLFDNSKVEKKDLFTGTYMPLTELTGGYRVLSYIDPSEPDHEK 183
           ITSDSRVVVLLDGKSFPVLFD+SKVEKKDLFTGTYMP+TELTGGYRVLSYIDPSEPDH K
Sbjct: 122 ITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAK 181

Query: 184 LKQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAAAGKAEYNASGEQAAFNFLARSLF 243
           LKQL+FFLLKHRRDKI+PEFHSTFSELFETLEKDLAA+G+AEYNA GEQAAFNFLARSLF
Sbjct: 182 LKQLVFFLLKHRRDKIMPEFHSTFSELFETLEKDLAASGRAEYNAPGEQAAFNFLARSLF 241

Query: 244 GANPVDTKLGRDAPKLISKWVLFQLGPVLRLGLPKVVEELLLRTVRLPPALIKADYRRLY 303
           GA+PVD+KLGRDAPKLI+KWVLFQLGPVL LGLPKVVEELLLRTVRLPPALIKADYRRLY
Sbjct: 242 GADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLY 301

Query: 304 DFFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKILFPNMIKWIGRAGVNLHT 363
           +FFY+SSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKI FPNMIKWIGRAGVNLHT
Sbjct: 302 EFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHT 361

Query: 364 QLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDA 423
           QLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDA
Sbjct: 362 QLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDA 421

Query: 424 AFEIKEGEMLCGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPTVQ 483
           AFEIKEGEM+CGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSP+VQ
Sbjct: 422 AFEIKEGEMICGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPSVQ 481

Query: 484 NKQCAGKDFIVFISRLLVVELFLRYDSFDIEATNTPLGAAVTVTSLKKASF 533
           NKQCAGKDFIVFISRL+VVELFLRYDSFDIEA+NTPLGAAVTVTSLKKASF
Sbjct: 482 NKQCAGKDFIVFISRLMVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 532

[Close]

BLAST of Lsi08G014260 vs. NCBI nr
Match: gi|21616113|gb|AAM66138.1|AF081954_1 (allene oxide synthase [Cucumis melo])

HSP 1 Score: 988.8 bits (2555), Expect = 3.7e-285
Identity = 495/536 (92.35%), Postives = 516/536 (96.27%), Query Frame = 1


Query: 4   TSIIIPSLQTQLRFPS-QETPLRSRSRVGFVSLRPICAADRVSSTSSSSVQVPQRTVSPP 63
           +SI+IPSLQ  LRFPS QETP RSRSRVGFVS+RPI A D VSS+SSSS+QVPQR VSPP
Sbjct: 2   SSIVIPSLQPHLRFPSSQETPQRSRSRVGFVSIRPIYATDGVSSSSSSSLQVPQRIVSPP 61

Query: 64  EPTKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFI 123
           EPTKLPLRK+PGDYGPPMFGALKDR DYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFI
Sbjct: 62  EPTKLPLRKVPGDYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFI 121

Query: 124 TSDSRVVVLLDGKSFPVLFDNSKVEKKDLFTGTYMPLTELTGGYRVLSYIDPSEPDHEKL 183
           TSDSRVVVLLDGKSFPVLFD+SKVEKKDLFTGTYMP+TELTGGYRVLSYIDPSEPDH KL
Sbjct: 122 TSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAKL 181

Query: 184 KQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAAAGKAEYNASGEQAAFNFLARSLFG 243
           KQLIFFLLKHRRDKI+PEFHSTFSELFETLEKDLAAAG+AEYNASGEQAAFNFLARSLFG
Sbjct: 182 KQLIFFLLKHRRDKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLARSLFG 241

Query: 244 ANPVDTKLGRDAPKLISKWVLFQLGPVLRLGLPKVVEELLLRTVRLPPALIKADYRRLYD 303
           A+PVD+KLGRDAPKLI+KWVLFQLGPVL LGLPKVVEELLLRTVRLPPALIKADYRRLYD
Sbjct: 242 ADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLYD 301

Query: 304 FFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKILFPNMIKWIGRAGVNLHTQ 363
           FFY+SSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKI FPNMIKWIGRAGVNLHT+
Sbjct: 302 FFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHTR 361

Query: 364 LAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDAA 423
           LAREIRTAVKANGGKITMGAMEQMPLMKSVVYEA RIEPPVPVQYGRAKKDLVVESHDAA
Sbjct: 362 LAREIRTAVKANGGKITMGAMEQMPLMKSVVYEALRIEPPVPVQYGRAKKDLVVESHDAA 421

Query: 424 FEIKEGEMLCGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPTVQN 483
           FEIKEGE++CGYQPFATRDPKIFDRADE VPDRFTG+GEELLKHV+WSNGPETQSP+VQN
Sbjct: 422 FEIKEGEVICGYQPFATRDPKIFDRADELVPDRFTGEGEELLKHVIWSNGPETQSPSVQN 481

Query: 484 KQCAGKDFIVFISRLLVVELFLRYDSFDIEATNTPLG------AAVTVTSLKKASF 533
           KQCAGKDFIVFISRLLVVELFLRYDSFDIEA+NTPLG      AAVTVTSLKKASF
Sbjct: 482 KQCAGKDFIVFISRLLVVELFLRYDSFDIEASNTPLGAAVTVSAAVTVTSLKKASF 537

[Close]

BLAST of Lsi08G014260 vs. NCBI nr
Match: gi|619498821|gb|AHY03308.1| (allene oxide synthase [Camellia sinensis])

HSP 1 Score: 760.0 bits (1961), Expect = 2.8e-216
Identity = 373/534 (69.85%), Postives = 448/534 (83.90%), Query Frame = 1


Query: 1   MSTTSIIIPSLQTQLRFPSQETPLRSRSRVGFVSLRPICAADRVSSTSSSSVQVPQRTVS 60
           M+++S+  PSLQ  L+FP+Q +   S  R+    +RPI A     S S      P   V 
Sbjct: 1   MASSSLTFPSLQ--LQFPTQRSSKPSSRRL---IVRPITA-----SISEKPSATPTPAVK 60

Query: 61  PPE-PTKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPG 120
           PP+ PT LP+RKIPG+YG P+ G LKDR DYFYNQGR+EY KSR+ +Y+STV+R NMPPG
Sbjct: 61  PPQPPTNLPIRKIPGNYGIPLIGPLKDRLDYFYNQGRDEYFKSRVQKYQSTVFRANMPPG 120

Query: 121 PFITSDSRVVVLLDGKSFPVLFDNSKVEKKDLFTGTYMPLTELTGGYRVLSYIDPSEPDH 180
           PFI S+  VVVLLDGKSFP+LFD +KV+K+D+FTGTYMP TELTGGYRVLSY+DPSEP+H
Sbjct: 121 PFIASNPNVVVLLDGKSFPILFDVTKVKKRDVFTGTYMPSTELTGGYRVLSYLDPSEPNH 180

Query: 181 EKLKQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAAAGKAEYNASGEQAAFNFLARS 240
            KLKQL+FFLLK  RD+++PEF ++F+ELFE LE +L + GKA +N + +QAAFNFLARS
Sbjct: 181 AKLKQLMFFLLKSSRDRVIPEFQTSFTELFEMLESELGSEGKASFNEANDQAAFNFLARS 240

Query: 241 LFGANPVDTKLGRDAPKLISKWVLFQLGPVLRLGLPKVVEELLLRTVRLPPALIKADYRR 300
           L+G NPVDTKLG D PKL++KWV+FQLGP+L LGLPK VEEL++ T RLPPAL+K DY+R
Sbjct: 241 LYGTNPVDTKLGIDGPKLVTKWVVFQLGPLLILGLPKFVEELVIHTFRLPPALVKKDYQR 300

Query: 301 LYDFFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKILFPNMIKWIGRAGVNL 360
           LYDFFY SS  V +EA+R+GISREEACHNL+F TCFNSFGGMKILFPNMIK IGRAGVNL
Sbjct: 301 LYDFFYDSSTLVLDEAERIGISREEACHNLIFATCFNSFGGMKILFPNMIKRIGRAGVNL 360

Query: 361 HTQLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESH 420
           HTQLA EIR+AV++NGG++TM AMEQMPLMKSVVYE+ RIEPP+ +QYGRAK+DLV+ESH
Sbjct: 361 HTQLAEEIRSAVRSNGGEVTMAAMEQMPLMKSVVYESLRIEPPISLQYGRAKRDLVIESH 420

Query: 421 DAAFEIKEGEMLCGYQPFATRDPKIFDRADEFVPDRFTG-DGEELLKHVLWSNGPETQSP 480
           DAAFE+KEGE+L G+QPFAT+DPKIF+R +EFV DRF G +GE LL+HVLWSNGPET+SP
Sbjct: 421 DAAFEVKEGELLFGFQPFATKDPKIFERPEEFVADRFVGEEGERLLRHVLWSNGPETESP 480

Query: 481 TVQNKQCAGKDFIVFISRLLVVELFLRYDSFDIEATNTPLGAAVTVTSLKKASF 533
           TV NKQCAGKDF+VF+SRLLVVELF RYDSF+IE   +PLGA VTVTSLK+ASF
Sbjct: 481 TVGNKQCAGKDFVVFVSRLLVVELFRRYDSFEIEVGQSPLGAKVTVTSLKRASF 524

[Close]

BLAST of Lsi08G014260 vs. NCBI nr
Match: gi|703136134|ref|XP_010106073.1| (Allene oxide synthase [Morus notabilis])

HSP 1 Score: 756.1 bits (1951), Expect = 4.0e-215
Identity = 376/533 (70.54%), Postives = 444/533 (83.30%), Query Frame = 1


Query: 1   MSTTSIIIPSLQTQLRFPSQETPLRSRSRVGFVSLRPICAADRVSSTSSSSVQVPQRTVS 60
           M++TS+  PSLQ Q R  S+++  RS  R  FVS  PI A    S +  SSV VP   V 
Sbjct: 1   MASTSLAFPSLQPQFR-SSRKSHKRSNRR--FVSF-PIHA----SVSEKSSVPVPPAAVE 60

Query: 61  PPEPTKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGP 120
             EP+KLP+RKIPG YG P  G + DR DYFYNQGR+EY KSR+ +Y+STV+R N+PPGP
Sbjct: 61  QHEPSKLPIRKIPGKYGLPFIGPVADRLDYFYNQGRDEYFKSRIQKYQSTVFRANIPPGP 120

Query: 121 FITSDSRVVVLLDGKSFPVLFDNSKVEKKDLFTGTYMPLTELTGGYRVLSYIDPSEPDHE 180
            ITS+ RV+VLLDGKSFPVLFD +KVEKKDLFTGT+MP T+LTGGYRVLSY+DPSEP+H 
Sbjct: 121 LITSNPRVIVLLDGKSFPVLFDVTKVEKKDLFTGTFMPSTDLTGGYRVLSYLDPSEPNHG 180

Query: 181 KLKQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAAAGKAEYNASGEQAAFNFLARSL 240
           K+K LIFFLLK RRDK++PEFHS+++ELFETLE +LAA GK+ +  + +QAAFNFL+R+L
Sbjct: 181 KIKSLIFFLLKSRRDKVIPEFHSSYTELFETLESELAAKGKSNFGEANDQAAFNFLSRAL 240

Query: 241 FGANPVDTKLGRDAPKLISKWVLFQLGPVLRLGLPKVVEELLLRTVRLPPALIKADYRRL 300
           +G NP DTKLG D P L+ KWV+FQL P+L LGLPK VEEL L + RLPP L+K+DY+RL
Sbjct: 241 YGTNPADTKLGNDGPGLVQKWVVFQLSPILVLGLPKFVEELTLHSFRLPPFLVKSDYQRL 300

Query: 301 YDFFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKILFPNMIKWIGRAGVNLH 360
           YDFFY+SS  V +EA+RLGISREEACHNLLF TCFNSFGGMKILFPNM+K IGRAGV LH
Sbjct: 301 YDFFYESSGHVLDEAERLGISREEACHNLLFATCFNSFGGMKILFPNMLKQIGRAGVKLH 360

Query: 361 TQLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHD 420
           T+LAREIRT V++NGGK+TM AMEQMPLMKSVVYE+ RIEPPV +Q+G+AK+D V+ESHD
Sbjct: 361 TELAREIRTVVRSNGGKVTMAAMEQMPLMKSVVYESLRIEPPVSLQFGKAKRDFVIESHD 420

Query: 421 AAFEIKEGEMLCGYQPFATRDPKIFDRADEFVPDRFTG-DGEELLKHVLWSNGPETQSPT 480
           AAFEIKEGEM+ GYQPFAT+DPKIFDRA+EFVPDRF G +GE LLKHVLWSNGPET+SPT
Sbjct: 421 AAFEIKEGEMIFGYQPFATKDPKIFDRAEEFVPDRFVGEEGERLLKHVLWSNGPETESPT 480

Query: 481 VQNKQCAGKDFIVFISRLLVVELFLRYDSFDIEATNTPLGAAVTVTSLKKASF 533
           V NKQCAGKD +V  SRLLVVELFL YDSF+IE   + LG+AVTVTSLK+ASF
Sbjct: 481 VGNKQCAGKDLVVLFSRLLVVELFLLYDSFEIEVGTSALGSAVTVTSLKRASF 525

The following BLAST results are available for this feature:

BLAST of Lsi08G014260 vs. Swiss-Prot


Match Name	E-value	Identity	Description
AOS1_SOLLC	2.5e-209	66.04	Allene oxide synthase 1, chloroplastic OS=Solanum lycopersicum GN=AOS1 PE=2 SV=1	[more]
CP74_LINUS	4.2e-196	64.61	Allene oxide synthase, chloroplastic OS=Linum usitatissimum GN=CYP74A PE=1 SV=1	[more]
AOS2_SOLLC	7.1e-196	63.41	Allene oxide synthase 2, chloroplastic OS=Solanum lycopersicum GN=AOS2 PE=1 SV=1	[more]
CP74A_ARATH	2.5e-193	66.46	Allene oxide synthase, chloroplastic OS=Arabidopsis thaliana GN=CYP74A PE=1 SV=3	[more]
C74A2_PARAR	2.4e-183	64.54	Allene oxide synthase OS=Parthenium argentatum GN=CYP74A2 PE=1 SV=2	[more]

BLAST of Lsi08G014260 vs. TrEMBL


Match Name	E-value	Identity	Description
U5YTX9_CUCSA	2.8e-287	93.60	Allene oxide synthase OS=Cucumis sativus GN=AOS PE=2 SV=1	[more]
Q8L5M5_CUCME	2.6e-285	92.35	Allene oxide synthase OS=Cucumis melo GN=AOS PE=2 SV=1	[more]
A0A023UKW8_CAMSI	1.9e-216	69.85	Allene oxide synthase OS=Camellia sinensis GN=AOS2 PE=2 SV=1	[more]
W9S158_9ROSA	2.8e-215	70.54	Allene oxide synthase OS=Morus notabilis GN=L484_021251 PE=3 SV=1	[more]
A0A0U5B3Q9_CAMSI	1.8e-214	69.94	CYP74A52 OS=Camellia sinensis GN=cytochrome P450 PE=2 SV=1	[more]

BLAST of Lsi08G014260 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G42650.1	1.4e-194	66.46	allene oxide synthase	[more]
AT4G15440.1	1.1e-74	38.96	hydroperoxide lyase 1	[more]

BLAST of Lsi08G014260 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659087845\|ref\|XP_008444665.1\|	8.0e-288	93.58	PREDICTED: allene oxide synthase [Cucumis melo]	[more]
gi\|566006191\|ref\|NP_001274390.1\|	3.9e-287	93.60	allene oxide synthase [Cucumis sativus]	[more]
gi\|21616113\|gb\|AAM66138.1\|AF081954_1	3.7e-285	92.35	allene oxide synthase [Cucumis melo]	[more]
gi\|619498821\|gb\|AHY03308.1\|	2.8e-216	69.85	allene oxide synthase [Camellia sinensis]	[more]
gi\|703136134\|ref\|XP_010106073.1\|	4.0e-215	70.54	Allene oxide synthase [Morus notabilis]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004497	monooxygenase activity
GO:0020037	heme binding
GO:0005506	iron ion binding
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

Vocabulary: **INTERPRO**
Term	Definition
IPR002403	Cyt_P450_E_grp-IV
IPR001128	Cyt_P450

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0031407	oxylipin metabolic process
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0016125	sterol metabolic process
biological_process	GO:0009611	response to wounding
biological_process	GO:0009695	jasmonic acid biosynthetic process
biological_process	GO:0009753	response to jasmonic acid
cellular_component	GO:0009941	chloroplast envelope
cellular_component	GO:0009535	chloroplast thylakoid membrane
cellular_component	GO:0010287	plastoglobule
cellular_component	GO:0005739	mitochondrion
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016829	lyase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0047987	hydroperoxide dehydratase activity
molecular_function	GO:0020037	heme binding
molecular_function	GO:0009978	allene oxide synthase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G014260.1	Lsi08G014260.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	GENE3D	G3DSA:1.10.630.10		coord: 362..513 score: 1.9
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 361..459 score: 1.5
IPR001128	Cytochrome P450	unknown	SSF48264	Cytochrome P450	coord: 67..507 score: 1.7
IPR002403	Cytochrome P450, E-class, group IV	PRINTS	PR00465	EP450IV	coord: 386..402 score: 1.4E-5coord: 485..503 score: 1.4E-5coord: 439..457 score: 1.
None	No IPR available	PANTHER	PTHR24286	FAMILY NOT NAMED	coord: 40..515 score: 3.6E
None	No IPR available	PANTHER	PTHR24286:SF50	ALLENE OXIDE SYNTHASE, CHLOROPLASTIC	coord: 40..515 score: 3.6E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi08G014260	Csa7G075590	Cucumber (Chinese Long) v2	culsiB537
Lsi08G014260	Csa2G360780	Cucumber (Chinese Long) v2	culsiB142
Lsi08G014260	CSPI07G08060	Wild cucumber (PI 183967)	cpilsiB552
Lsi08G014260	CSPI02G19630	Wild cucumber (PI 183967)	cpilsiB141
Lsi08G014260	Cucsa.232480	Cucumber (Gy14) v1	cgylsiB379
Lsi08G014260	CmaCh05G002170	Cucurbita maxima (Rimu)	cmalsiB739
Lsi08G014260	CmaCh12G005010	Cucurbita maxima (Rimu)	cmalsiB184
Lsi08G014260	CmaCh19G006410	Cucurbita maxima (Rimu)	cmalsiB498
Lsi08G014260	CmoCh05G002230	Cucurbita moschata (Rifu)	cmolsiB731
Lsi08G014260	CmoCh11G013280	Cucurbita moschata (Rifu)	cmolsiB140
Lsi08G014260	CmoCh19G006540	Cucurbita moschata (Rifu)	cmolsiB482
Lsi08G014260	Cla022526	Watermelon (97103) v1	lsiwmB465
Lsi08G014260	Cla015969	Watermelon (97103) v1	lsiwmB492
Lsi08G014260	MELO3C010910	Melon (DHL92) v3.5.1	lsimeB469
Lsi08G014260	ClCG02G005440	Watermelon (Charleston Gray)	lsiwcgB428
Lsi08G014260	ClCG08G015460	Watermelon (Charleston Gray)	lsiwcgB438
Lsi08G014260	MELO3C010910.2	Melon (DHL92) v3.6.1	lsimedB489
Lsi08G014260	Bhi04G000261	Wax gourd	lsiwgoB591
Lsi08G014260	Cp4.1LG11g01710	Cucurbita pepo (Zucchini)	cpelsiB100
Lsi08G014260	Cp4.1LG07g04430	Cucurbita pepo (Zucchini)	cpelsiB685
Lsi08G014260	CsGy2G019490	Cucumber (Gy14) v2	cgyblsiB129
Lsi08G014260	CsGy7G007610	Cucumber (Gy14) v2	cgyblsiB505
Lsi08G014260	Carg11827	Silver-seed gourd	carlsiB164
Lsi08G014260	Carg04079	Silver-seed gourd	carlsiB898
Lsi08G014260	CsaV3_2G028330	Cucumber (Chinese Long) v3	cuclsiB155

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi08G014260	Bottle gourd (USVL1VR-Ls)	lsilsiB088
Lsi08G014260	Cucumber (Gy14) v1	cgylsiB130
Lsi08G014260	Cucurbita maxima (Rimu)	cmalsiB032
Lsi08G014260	Cucurbita maxima (Rimu)	cmalsiB456
Lsi08G014260	Cucurbita maxima (Rimu)	cmalsiB810
Lsi08G014260	Cucurbita moschata (Rifu)	cmolsiB020
Lsi08G014260	Cucurbita moschata (Rifu)	cmolsiB175
Lsi08G014260	Cucurbita moschata (Rifu)	cmolsiB442
Lsi08G014260	Cucurbita moschata (Rifu)	cmolsiB796
Lsi08G014260	Cucurbita pepo (Zucchini)	cpelsiB477
Lsi08G014260	Cucurbita pepo (Zucchini)	cpelsiB644
Lsi08G014260	Wild cucumber (PI 183967)	cpilsiB239
Lsi08G014260	Wild cucumber (PI 183967)	cpilsiB486
Lsi08G014260	Melon (DHL92) v3.5.1	lsimeB491
Lsi08G014260	Watermelon (Charleston Gray)	lsiwcgB431
Lsi08G014260	Melon (DHL92) v3.6.1	lsimedB507
Lsi08G014260	Melon (DHL92) v3.6.1	lsimedB510
Lsi08G014260	Silver-seed gourd	carlsiB096
Lsi08G014260	Silver-seed gourd	carlsiB746
Lsi08G014260	Cucumber (Chinese Long) v3	cuclsiB336
Lsi08G014260	Cucumber (Chinese Long) v3	cuclsiB507
Lsi08G014260	Watermelon (97103) v2	lsiwmbB423
Lsi08G014260	Watermelon (97103) v2	lsiwmbB444
Lsi08G014260	Wax gourd	lsiwgoB616