Lsi08G005630 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi08G005630
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Methenyltetrahydrofolate synthase domain-containing
Location	chr08 : 13824850 .. 13828778 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGAATATTAAAAAAATAAAAAATAAAAATCTTATCTTCATCCGTGGCGAACTATCCGCGAGGCGCGAACACTCGAACAGTACACTGAAATTCTCGTTAATGGATTCCAATCTCCTGCAATTATCCCACACACTATCACTCAAATCCACATTCCTCAACGCAATTCGAAGATCCCCTTCCATCTCTTCAATCTCCATCAGAAGAAATTTTCAGTTCCAGAGGCACTTCAAGCTGGAGAGCAGTAAAAATGGTGGCAGAGAAACCCAACGGGATTGTGCTTTCGACGAAGTAGCATTCGAGGCCGAGAGATCTCGCCTCGACGCCCAAGCTGTAAAATCCATGGCCGAAGCTTCAATTAGGGACACTGAAGGCGCTTCCGACGACGACCCGAAAGCTTGGAAGTGGATCATCCGAAAACGAGTTTGGGATTTCATGGAGTCACAAAATATCGCGGCAAATCCCCGTCCCGTTCACCATCGAATCCCTAATTTCCTTGGCGCCATGGAAGCTGCCAATAGAGTGAGCCTCCCCAATTCCTGAATTGTTTTCTTGGAAAGAAAATGTTTGAGTCGATCTTTTCAGTATTGGAAATTTTGATTCCTTCCGCGTATACCTGATGAAATGGAGTTGTATCAGTTGTGCGATTTGGAAGTATTCAGGGATTCACAGTGCGTGAAGGTTAATCCGGATTCTCCTCAGAAGGGTGTGAGGCTTCTTACACTTACGGGTCAGTTCTGCTGATACTTTTATGATTTATCCTGCTGTTCTTCTCGTTTGGTTGTTTGTTTTCTTTAGATTGTCTACCTACCATCGTCAAGAGACTATGTTTCGAGCTGTTAATGAATTCGAACACTCAATGCTAGCGAACAATACTATTTCGATATGATTTAGATTGGCATTTTTTATGTCATATCTTCTCTGGTGACTGAATGATAGTCTGGTCAATTGCAAGTCCGGTCTCTGAACTTGCGTGTGTCTATTTGGTTCGTGAAGTTTCAAAAGTGTCTAATAATCTTGAAGTTTCAATTGTGTTCAATAACCTGAACTTTAAAAACGGTTTAATGGATCTCCAAAATTTTAATTTCATGTCCAATATGTTTTTGACTTGCTCGATATTTCAAAAATTAATGAGTCTACCCGACACCAAATTGAAAATTTAAAGTTCTATCGTACACAAATTTCAAATTCGTATCTAATGGATCAATTATTTTTTGTGAAAATTTTGAATACATCATAGATTTATTAGACACAAAATAAAAAGTTTTTAGACCTAGTGACCTATTAGATACATAATTGAACACTTTTGAAAATTCAAAAGACTAAACACACGCAAACCTAAAAGTTCAGGTACTAAACTTCTAATTTAAACTTAATGTTTCTTTCTTTTCTTGAAGAGACTTTTGTCTGTGCATGATTCAACATCTGTGTACCTATATCTGCAAAAATATGATAATTGATTAGTAAATAAACGATGGAGATAATTCTTTTATTTTCTGGATAACCCTGTAGTACTTATCAAGAAGAGAGTTCACTCAAGGATGTTAAACCTTACAAAGCCTTGTGCAAAATTGTATGGCAAAAGGCCTGCATGAGTGATGTGAGGAGGGGAAACTTCAAAATATGTCAACTGGTGATGTCTTAGTTTATAACTAAAGCTAGGAAAGACGAGATCTACTGAAATTATGAATGTCTAATGGTAATTTGGTGGTTGGCTGTCTCAACGTCGACATTGTCAGATTTAGTTACAATTTTCTGGTGGTTAAATCATACAGTGTAAGCTTCGACTGAATCGTGAACATCATTTCACGACTTAGCGAGTTGTATGTAACGTCTTTGATTCTGAAAAAGCATGAAAGCTTTCTCATGTTAAATCACAAACTAAATGGTATTCTCATATGATCAGGTGGCAAAAAACTGTTAACACCTCAGCCTCGGTTAAGAACAGGGTTTTTCTCCATAGTCGAATCAGGGATGTTGACTCCGGCTACCATCAACGAGGCCTGCACTTCTGTTGGGGTAGCAAAGTATGGAAAGCCTATTGGACTGGATGAAAAGATCAAAGTTGATCTGATCATCATTGGTTCTGTTGCTGTTGACCCCAGAACGGGTGCTCGGCTTGGCAAGGGAGAGGTAAAAGTGTTACTGTTGGCTTTACCTTTTACATCGAAAATGGCTGGTTATAAAACATATTTGCTCAGAAGATTAGTCCTCAGTCTTCAGACATCCTCTCTTAGTATCTTCCCACATCTTCTCTACAGCCATCTATTTGTTTGCACATAACTTACATCTATGGCTCATTTCTTGCCTTCTTTGCCACTTTGAAAATGGAATGGATTGATTAAGGAACTCTTTAGATTGACTTTCAAAGTTCTTAGAAAGGTGTTTTTAAGTGCAAATGAAGTGTTTATAAATACTTAGAAAGTCAATTCAAATAGACCTCAACAAGGGAAATAGTATGTTTGATTCTAACTATGCTTCTTTAGGCCACTTTATAAAAATACATCCAATAGGTTTTTGAACTTTAAAAAGTGCCTAATCGGTCTTTAGACTTTCAATTTTGTGTCTAATAGATTTTGACTTATTTGATATTTTTATAATTCACAGCTCTAGTAGACACAAAATTGAATGTAAACTTAAAAGCTTATAAACTAAACTTAAAATTTACTCTATAAAATGCAGGGATTTGCAGAACTAGAATATGGAATGCTGCGGTACATGGGAGCCATTGATGACTCGACTCCAATAGTTACTTCTGGTTGTGTAACTACTCTGCTTCTATCTTATATTGTAGTTTCTCATTCTTTTCATTATTTTCAGCAACAATTTCTTGTGTAACATAATTTAACAGGTGGAGATAAATTTCAGTGCTTAATATCGAAACGTTCTTTACTATCAACTTAGATCGTGCAACTTTAGCTGTGTTTAGATAGTTCAGCAGTTGACTTCCCGATATATATAAATTTCAAACCTCCCCCATCCTCAAGAAAAAGAGAGCTTGTTTTCAAATTTACTTGCGTAGAATTATGGGTTGAAGACAACATTTGTCAAGCAGTTGAGATAATTTGTGTACAGAGGGCTGTTGGTTTGAGAACTATGGCGATAAAAATAACGTTGAAATGTTTTAGATACAAATGGATAGCTTACATGTTATTGAGCTCCCCTCTTTGCTTCACACATGTTAAAGTTCATATTGTCTATGTGCTTTTGGCTGTGCATTTTTATCAAACCTCCTGTCGTAATGCAGATTTCCAATTCTGTTGCAGTGCACGATTGCCAATTGGTTGACGATATTCCAGTTGAGAAGCTATTAGTCCATGACGTGCCTGTAGACATCGTATGCACTCCGACGCAGGTCATTTTAACCAACACGAGAATCCCCAAACCCCAAGGTTAGCAGCTTATTTGTAGACTTTTTAAGCAAAATTAATCATAGTAAAGCTGCCTTTTCTTTTTATTGCATATCTCAAAAGATTAGTGTAGCACATTTGAATTTGCTTAGATTGAAGGGACATGAGTTTATGAACAAATTTGTAATATCAACTGATAGTGTCTCATATATACAACCCAAAATGTTTTGAATTGCAGGCATTTACTGGGAAATGTTGTCTCCCGAGAAGCTGAGCCAAGTTCGAATACTCAGGGAGCTCAAACGTCGTATCGAACGGGAGACTGGAAAACCACTGCCATGTGGTCCGTCGGAGAAACTACCACCTACAGCTCAACGAAGCTCGAAACCTGGAAAACGTGCATCCCCTAAGAAGTGATGAAGGGGAAAATGGAGAAAGAAACAAACCTAAATTAGTTTTTTTCCCCCCCTTTTTCCTTCATTATAAAGTTTCATTGTTGAATAAACACTGTCCTAAAACAGACGAGGAAGATTTGAAAGTAACAAAGAAATGAAAATATAAGAGGGAGTGTTTTCTTCCCTAA

mRNA sequence

AGAATATTAAAAAAATAAAAAATAAAAATCTTATCTTCATCCGTGGCGAACTATCCGCGAGGCGCGAACACTCGAACAGTACACTGAAATTCTCGTTAATGGATTCCAATCTCCTGCAATTATCCCACACACTATCACTCAAATCCACATTCCTCAACGCAATTCGAAGATCCCCTTCCATCTCTTCAATCTCCATCAGAAGAAATTTTCAGTTCCAGAGGCACTTCAAGCTGGAGAGCAGTAAAAATGGTGGCAGAGAAACCCAACGGGATTGTGCTTTCGACGAAGTAGCATTCGAGGCCGAGAGATCTCGCCTCGACGCCCAAGCTGTAAAATCCATGGCCGAAGCTTCAATTAGGGACACTGAAGGCGCTTCCGACGACGACCCGAAAGCTTGGAAGTGGATCATCCGAAAACGAGTTTGGGATTTCATGGAGTCACAAAATATCGCGGCAAATCCCCGTCCCGTTCACCATCGAATCCCTAATTTCCTTGGCGCCATGGAAGCTGCCAATAGATTGTGCGATTTGGAAGTATTCAGGGATTCACAGTGCGTGAAGGTTAATCCGGATTCTCCTCAGAAGGGTGTGAGGCTTCTTACACTTACGGGTGGCAAAAAACTGTTAACACCTCAGCCTCGGTTAAGAACAGGGTTTTTCTCCATAGTCGAATCAGGGATGTTGACTCCGGCTACCATCAACGAGGCCTGCACTTCTGTTGGGGTAGCAAAGTATGGAAAGCCTATTGGACTGGATGAAAAGATCAAAGTTGATCTGATCATCATTGGTTCTGTTGCTGTTGACCCCAGAACGGGTGCTCGGCTTGGCAAGGGAGAGATTTCCAATTCTGTTGCAGTGCACGATTGCCAATTGGTTGACGATATTCCAGTTGAGAAGCTATTAGTCCATGACGTGCCTGTAGACATCGTATGCACTCCGACGCAGGTCATTTTAACCAACACGAGAATCCCCAAACCCCAAGGCATTTACTGGGAAATGTTGTCTCCCGAGAAGCTGAGCCAAGTTCGAATACTCAGGGAGCTCAAACGTCGTATCGAACGGGAGACTGGAAAACCACTGCCATGTGGTCCGTCGGAGAAACTACCACCTACAGCTCAACGAAGCTCGAAACCTGGAAAACGTGCATCCCCTAAGAAGTGATGAAGGGGAAAATGGAGAAAGAAACAAACCTAAATTAGTTTTTTTCCCCCCCTTTTTCCTTCATTATAAAGTTTCATTGTTGAATAAACACTGTCCTAAAACAGACGAGGAAGATTTGAAAGTAACAAAGAAATGAAAATATAAGAGGGAGTGTTTTCTTCCCTAA

Coding sequence (CDS)

ATGGATTCCAATCTCCTGCAATTATCCCACACACTATCACTCAAATCCACATTCCTCAACGCAATTCGAAGATCCCCTTCCATCTCTTCAATCTCCATCAGAAGAAATTTTCAGTTCCAGAGGCACTTCAAGCTGGAGAGCAGTAAAAATGGTGGCAGAGAAACCCAACGGGATTGTGCTTTCGACGAAGTAGCATTCGAGGCCGAGAGATCTCGCCTCGACGCCCAAGCTGTAAAATCCATGGCCGAAGCTTCAATTAGGGACACTGAAGGCGCTTCCGACGACGACCCGAAAGCTTGGAAGTGGATCATCCGAAAACGAGTTTGGGATTTCATGGAGTCACAAAATATCGCGGCAAATCCCCGTCCCGTTCACCATCGAATCCCTAATTTCCTTGGCGCCATGGAAGCTGCCAATAGATTGTGCGATTTGGAAGTATTCAGGGATTCACAGTGCGTGAAGGTTAATCCGGATTCTCCTCAGAAGGGTGTGAGGCTTCTTACACTTACGGGTGGCAAAAAACTGTTAACACCTCAGCCTCGGTTAAGAACAGGGTTTTTCTCCATAGTCGAATCAGGGATGTTGACTCCGGCTACCATCAACGAGGCCTGCACTTCTGTTGGGGTAGCAAAGTATGGAAAGCCTATTGGACTGGATGAAAAGATCAAAGTTGATCTGATCATCATTGGTTCTGTTGCTGTTGACCCCAGAACGGGTGCTCGGCTTGGCAAGGGAGAGATTTCCAATTCTGTTGCAGTGCACGATTGCCAATTGGTTGACGATATTCCAGTTGAGAAGCTATTAGTCCATGACGTGCCTGTAGACATCGTATGCACTCCGACGCAGGTCATTTTAACCAACACGAGAATCCCCAAACCCCAAGGCATTTACTGGGAAATGTTGTCTCCCGAGAAGCTGAGCCAAGTTCGAATACTCAGGGAGCTCAAACGTCGTATCGAACGGGAGACTGGAAAACCACTGCCATGTGGTCCGTCGGAGAAACTACCACCTACAGCTCAACGAAGCTCGAAACCTGGAAAACGTGCATCCCCTAAGAAGTGA

Protein sequence

MDSNLLQLSHTLSLKSTFLNAIRRSPSISSISIRRNFQFQRHFKLESSKNGGRETQRDCAFDEVAFEAERSRLDAQAVKSMAEASIRDTEGASDDDPKAWKWIIRKRVWDFMESQNIAANPRPVHHRIPNFLGAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLTGGKKLLTPQPRLRTGFFSIVESGMLTPATINEACTSVGVAKYGKPIGLDEKIKVDLIIIGSVAVDPRTGARLGKGEISNSVAVHDCQLVDDIPVEKLLVHDVPVDIVCTPTQVILTNTRIPKPQGIYWEMLSPEKLSQVRILRELKRRIERETGKPLPCGPSEKLPPTAQRSSKPGKRASPKK

Homology

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BLAST of Lsi08G005630 vs. Swiss-Prot
Match: 5FCLL_ARATH (5-formyltetrahydrofolate cyclo-ligase-like protein COG0212 OS=Arabidopsis thaliana GN=COG0212 PE=2 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 1.9e-112
Identity = 203/312 (65.06%), Postives = 243/312 (77.88%), Query Frame = 1


Query: 52  GRETQRDCAFDEVAFEAERSRLDAQAVKSMAEASIRDTEGASDDDPKAWKWIIRKRVWDF 111
           G E     AFD VA+EA+R  LDA A++ MAE + ++ E   D DPKAWKW+IRK++WD 
Sbjct: 43  GSENGDGVAFDAVAYEADRLSLDAAAMEDMAETAKKELESDPDSDPKAWKWVIRKKMWDL 102

Query: 112 MESQNIAANPRPVHHRIPNFLGAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLTG 171
           ME++N A +PRPVHHRIPNF+GA  AA +L +L+ FR +  VKVNPDSPQK +R LTL+G
Sbjct: 103 MEARNYAMSPRPVHHRIPNFVGASAAARKLAELDAFRMAMVVKVNPDSPQKQIRFLTLSG 162

Query: 172 GKKLLTPQPRLRTGFFSIVESGMLTPATINEACTSVGVAKYGKPIGLDEKIKVDLIIIGS 231
            KKLLTPQPRLRTGFFS++ES +L P TI EACTSVGVAKYG+ IGLDEKIKVDLI+IGS
Sbjct: 163 EKKLLTPQPRLRTGFFSVLESDLLKPETIMEACTSVGVAKYGRAIGLDEKIKVDLIVIGS 222

Query: 232 VAVDPRTGARLGKGE---------------ISNS----VAVHDCQLVDDIPVEKLLVHDV 291
           VAV+P+TGARLGKGE               I +S      VHDCQLVDDIP+EKL +HDV
Sbjct: 223 VAVNPQTGARLGKGEGFAELEYGMLRYMGAIDDSTPVVTTVHDCQLVDDIPLEKLAIHDV 282

Query: 292 PVDIVCTPTQVILTNTRIPKPQGIYWEMLSPEKLSQVRILRELKRRIERETGKPLPCGPS 345
           PVDI+CTPT+VI TNT IPKPQGIYW+ LSPEKL Q+RILRELK R+E++TG+ LP GPS
Sbjct: 283 PVDIICTPTRVIFTNTPIPKPQGIYWDKLSPEKLRQIRILRELKNRLEKKTGRKLPTGPS 342

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BLAST of Lsi08G005630 vs. Swiss-Prot
Match: MTHSD_DANRE (Methenyltetrahydrofolate synthase domain-containing protein OS=Danio rerio GN=mthfsd PE=2 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 6.5e-52
Identity = 111/245 (45.31%), Postives = 156/245 (63.67%), Query Frame = 1


Query: 101 KWIIRKRVWDFMESQNIAANPRPVHHRIPNFLGAMEAANRLCDLEVFRDSQCVKVNPDSP 160
           KW +R +VW+++E +N+A  PRPVH+RIPNF GA+EA N++  LE+F +S  VKV+PD P
Sbjct: 13  KWDVRHKVWNYIEVKNLANFPRPVHNRIPNFKGALEACNKVAQLEIFIESAVVKVDPDKP 72

Query: 161 QKGVRLLTLTGGKKLLTPQPRLRTGFFS-IVESGMLTPATINEACTSVGVAKYGKPIGLD 220
            +GVRL  L   K LL P PRLR G F+ I      T  T+    TS G+ ++  P+GLD
Sbjct: 73  MEGVRLAALKARKSLLVPTPRLRFGLFNRITPPKGATKETLRVCSTSQGIKEFSVPVGLD 132

Query: 221 EKIKVDLIIIGSVAVDPRTGARLGKGE---------------ISNS----VAVHDCQLVD 280
           +K++VDL+++GSVAV  + G R+GKGE               ++ S      VHDCQ++ 
Sbjct: 133 DKVQVDLVVVGSVAVSEK-GYRIGKGEGFADMEYAMMACMGSVTESTWVITVVHDCQVM- 192

Query: 281 DIPVEKLLVHDVPVDIVCTPTQVILTNTRIPKPQGIYWEMLSPEKLSQVRILRELKRRIE 326
           DIP E +  HD+ VD + T T+VI T  + PKPQGI W ML  E+L ++ IL++L R +E
Sbjct: 193 DIPEELIERHDLMVDFIITATRVIKTECKHPKPQGIIWSMLHKEELKKIPILKKL-RTLE 252

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BLAST of Lsi08G005630 vs. Swiss-Prot
Match: MTHSD_BOVIN (Methenyltetrahydrofolate synthase domain-containing protein OS=Bos taurus GN=MTHFSD PE=2 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 2.7e-50
Identity = 116/261 (44.44%), Postives = 151/261 (57.85%), Query Frame = 1


Query: 104 IRKRVWDFMESQNIAANPRPVHHRIPNFLGAMEAANRLCDLEVFRDSQCVKVNPDSPQKG 163
           IR+++WD+MESQN+A  PRPVHHRIPNF GA  AA     L+ F+ ++ +KVNPD+PQK 
Sbjct: 12  IREQIWDYMESQNLADFPRPVHHRIPNFKGASRAAEHFPRLQAFKMARTIKVNPDAPQKN 71

Query: 164 VRLLTLTGGKKLLTPQPRLRTGFFS-IVESGMLTPATINEACTSVGVAKYGKPIGLDEKI 223
            R   L   K LL P PRLRTG F+ I      T   + +  TS GV  Y  P+GLD K+
Sbjct: 72  ARFFVLESKKTLLVPTPRLRTGLFNKITPPPGATKDILRKCATSQGVRNYSTPVGLDSKV 131

Query: 224 KVDLIIIGSVAVDPRTGARLGKGE---------------ISNS----VAVHDCQLVDDIP 283
            VDL+++GSVAV  + G R+GKGE               +S        VHDCQ+V DIP
Sbjct: 132 LVDLVVVGSVAVSEK-GWRIGKGEGYADLEYAMMVSMGAVSQGTPVVTIVHDCQVV-DIP 191

Query: 284 VEKLLVHDVPVDIVCTPTQVILTNTRIPKPQGIYWEMLSPEKLSQVRILRELKRRIERET 343
              L  HD+ VD + TPT+VI T    PKP GI W  +S E L ++ ILR L+ + E + 
Sbjct: 192 EALLEDHDLTVDYILTPTRVITTGCERPKPAGIAWSKISCEMLGKMPILRSLRHQ-EEQA 251

Query: 344 GKPLPCGPSEKLPPTAQRSSK 345
           GK +      + PP A RS +
Sbjct: 252 GKDVTLRDGPRSPPGATRSPR 269

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BLAST of Lsi08G005630 vs. Swiss-Prot
Match: MTHSD_XENLA (Methenyltetrahydrofolate synthase domain-containing protein OS=Xenopus laevis GN=mthfsd PE=2 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 7.9e-50
Identity = 120/276 (43.48%), Postives = 160/276 (57.97%), Query Frame = 1


Query: 96  DPKAWKWIIRKRVWDFMESQNIAANPRPVHHRIPNFLGAMEAANRLCDLEVFRDSQCVKV 155
           DP   KW IR++VWD++E+ N+A  PRPVHHRIPNF  + +A   + DLEVFR +  VKV
Sbjct: 8   DPGCSKWDIRQKVWDYIENNNLADFPRPVHHRIPNFKESHQACQNIIDLEVFRRTWEVKV 67

Query: 156 NPDSPQKGVRLLTLTGGKKLLTPQPRLRTGFFSIVESGMLTPATINEAC-TSVGVAKYGK 215
           +PD P +GVRL  L   K LL P PRLRTG F+ +         +   C TS GV  Y  
Sbjct: 68  DPDKPLEGVRLAALQARKTLLVPTPRLRTGLFNKITPPPGANKEVLRICSTSQGVKDYSV 127

Query: 216 PIGLDEKIKVDLIIIGSVAVDPRTGARLGKGE----------ISNS---------VAVHD 275
           P+GLD K++VDL+++GSVAV  + G R+GKGE          +S             VHD
Sbjct: 128 PMGLDAKVQVDLVVVGSVAVSVQ-GWRIGKGEGFADMEYAMMVSMGAVTEHTVVVTVVHD 187

Query: 276 CQLVDDIPVEKLLVHDVPVDIVCTPTQVILTNTRIPKPQGIYWEMLSPEKLSQVRILREL 335
           CQ+V DIP E L  HD+ VD + TPT++I T+ +  KPQGI W M++ E + ++ ILR L
Sbjct: 188 CQVV-DIPEELLEDHDLTVDFILTPTRIIKTDCKRAKPQGIIWSMITREMMGKIPILRNL 247

Query: 336 KRRIERETGKPLPCGPSEKLPPTAQRSSKPGKRASP 352
           + R ER  GK +              +S+ G R SP
Sbjct: 248 QDR-ERRAGKDVSLKDG--------NASEQGSRQSP 272

[Close]

BLAST of Lsi08G005630 vs. Swiss-Prot
Match: MTHSD_HUMAN (Methenyltetrahydrofolate synthase domain-containing protein OS=Homo sapiens GN=MTHFSD PE=1 SV=2)

HSP 1 Score: 196.1 bits (497), Expect = 6.7e-49
Identity = 117/263 (44.49%), Postives = 156/263 (59.32%), Query Frame = 1


Query: 104 IRKRVWDFMESQNIAANPRPVHHRIPNFLGAMEAANRLCDLEVFRDSQCVKVNPDSPQKG 163
           IR+++W +MESQN+A  PRPVHHRIPNF G+  A   + DL+VF  +Q VKV+PD P +G
Sbjct: 13  IREQIWGYMESQNLADFPRPVHHRIPNFKGSYLACQNIKDLDVFARTQEVKVDPDKPLEG 72

Query: 164 VRLLTLTGGKKLLTPQPRLRTGFFS-IVESGMLTPATINEACTSVGVAKYGKPIGLDEKI 223
           VRLL L   K LL P PRLRTG F+ I      T   + +  TS GV  Y  PIGLD ++
Sbjct: 73  VRLLVLQSKKTLLVPTPRLRTGLFNKITPPPGATKDILRKCATSQGVRNYSVPIGLDSRV 132

Query: 224 KVDLIIIGSVAVDPRTGARLGKGE---------------ISNS----VAVHDCQLVDDIP 283
            VDL+++GSVAV  + G R+GKGE               +S        VHDCQ+V DIP
Sbjct: 133 LVDLVVVGSVAVSEK-GWRIGKGEGYADLEYAMMVSMGAVSKETPVVTIVHDCQVV-DIP 192

Query: 284 VEKLLVHDVPVDIVCTPTQVILTNTRIPKPQGIYWEMLSPEKLSQVRILRELKRRIERET 343
            E +  HD+ VD + TPT+VI T  + PKP GI W  +S E + ++ ILR L+ R E++ 
Sbjct: 193 EELVEEHDITVDYILTPTRVIATGCKRPKPMGITWFKISLEMMEKIPILRSLRAR-EQQA 252

Query: 344 GKPLPC-GPSEKLPPTAQRSSKP 346
           GK +   G  + LP    + + P
Sbjct: 253 GKDVTLQGEHQHLPEPGCQQTVP 272

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BLAST of Lsi08G005630 vs. TrEMBL
Match: B9H7U6_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0005s21670g PE=4 SV=2)

HSP 1 Score: 453.0 bits (1164), Expect = 3.4e-124
Identity = 243/367 (66.21%), Postives = 277/367 (75.48%), Query Frame = 1


Query: 1   MDSNLLQLSHTLSLKSTFL-NAIRRSPSISSISIRRNFQ---FQRHFKLESSKNGGRETQ 60
           MDS L +++  L L  T L N    SPS+     RR      F  + KL SSK    +  
Sbjct: 1   MDSTLTRIAAPLPLTHTLLFNYPHSSPSLICFFSRRTHSKRLFNSNCKLGSSKRNAHDD- 60

Query: 61  RDCAFDEVAFEAERSRLDAQAVKSMAEASIRDTEGASDDDPKAWKWIIRKRVWDFMESQN 120
            +   DE A+EAER R DAQA  SMAE S R+ E +S+DDPKAWKW+IRKR+WD ME++N
Sbjct: 61  -NVVLDEKAYEAERLRFDAQARDSMAETSKREMETSSEDDPKAWKWVIRKRIWDLMEARN 120

Query: 121 IAANPRPVHHRIPNFLGAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLTGGKKLL 180
           IA NPRPVHHRIPNF+GA  AA  L  LEVF ++ CVKVNPDSPQK VR LTL+GGKKLL
Sbjct: 121 IAQNPRPVHHRIPNFVGASAAAKMLSGLEVFLEASCVKVNPDSPQKQVRFLTLSGGKKLL 180

Query: 181 TPQPRLRTGFFSIVESGMLTPATINEACTSVGVAKYGKPIGLDEKIKVDLIIIGSVAVDP 240
           TPQPRLRTGFFSI+ES ML+P+TINEACTSVGVAK G+PIGLDEKIKVDLI+IGSVAVDP
Sbjct: 181 TPQPRLRTGFFSILESHMLSPSTINEACTSVGVAKNGRPIGLDEKIKVDLIVIGSVAVDP 240

Query: 241 RTGARLGKGE---------------ISNS----VAVHDCQLVDDIPVEKLLVHDVPVDIV 300
           +TGARLGKGE               I +S     +VHDCQLV+DIPVEKLL+HDVPVDI+
Sbjct: 241 KTGARLGKGEGFAELEYGMLRYMGAIDDSTPVVTSVHDCQLVEDIPVEKLLIHDVPVDII 300

Query: 301 CTPTQVILTNTRIPKPQGIYWEMLSPEKLSQVRILRELKRRIERETGKPLPCGPSEKLPP 345
           CTP QVI TNT IPKPQGIYW+ LSPEKL+Q+RILRELK RIERETG+ LPCGPSEKLPP
Sbjct: 301 CTPDQVIFTNTNIPKPQGIYWDKLSPEKLAQIRILRELKSRIERETGQKLPCGPSEKLPP 360

[Close]

BLAST of Lsi08G005630 vs. TrEMBL
Match: B9SE88_RICCO (Catalytic, putative OS=Ricinus communis GN=RCOM_0959760 PE=4 SV=1)

HSP 1 Score: 441.4 bits (1134), Expect = 1.0e-120
Identity = 236/364 (64.84%), Postives = 271/364 (74.45%), Query Frame = 1


Query: 1   MDSNLLQLSHTLSLKSTF-LNAIRRSPSISSISIRRNFQFQRHFKLESSKNGGRETQRDC 60
           MDS L +++  LSLK +   +  R   S+ S  +     F  +  LE+S +  R    D 
Sbjct: 1   MDSTLTRIAAPLSLKQSIRFSNYRNFHSLPSRELSVQRLFSSNPNLETSSSNRRAPGNDT 60

Query: 61  AFDEVAFEAERSRLDAQAVKSMAEASIRDTEGASDDDPKAWKWIIRKRVWDFMESQNIAA 120
            FDE  FEAER RLDA+A +SMAE S  + +     DPKAWKW+IRKR+WDFME++NIA 
Sbjct: 61  VFDESTFEAERLRLDAKARESMAETSKLEMQL----DPKAWKWVIRKRIWDFMEARNIAQ 120

Query: 121 NPRPVHHRIPNFLGAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLTGGKKLLTPQ 180
           NPRPVHHRIPNF+GA  AA  +  LEVF D+ CVKVNPDSPQK VR LTL+GGK+LLTPQ
Sbjct: 121 NPRPVHHRIPNFIGASAAAQNMAGLEVFHDAGCVKVNPDSPQKQVRFLTLSGGKELLTPQ 180

Query: 181 PRLRTGFFSIVESGMLTPATINEACTSVGVAKYGKPIGLDEKIKVDLIIIGSVAVDPRTG 240
           PRLRTGFFS++ES ML P TI EACTSVGVAKYG+PIGLDEKIKVDLI+IGSVAVDP+TG
Sbjct: 181 PRLRTGFFSVLESDMLAPNTILEACTSVGVAKYGRPIGLDEKIKVDLIVIGSVAVDPKTG 240

Query: 241 ARLGKGE---------------ISNS----VAVHDCQLVDDIPVEKLLVHDVPVDIVCTP 300
           ARLGKGE               I +S     +VHDCQL DDIPVEKLL+HDVPVDI+CTP
Sbjct: 241 ARLGKGEGFAELEYGMLRYMGAIDDSTPVVTSVHDCQLTDDIPVEKLLIHDVPVDIICTP 300

Query: 301 TQVILTNTRIPKPQGIYWEMLSPEKLSQVRILRELKRRIERETGKPLPCGPSEKLPPTAQ 345
           TQVI TNT IPKPQGIYW+ LSPEKL Q+RILRELKRRIE ETG+ LP GPSEKLPPTAQ
Sbjct: 301 TQVIFTNTTIPKPQGIYWDKLSPEKLGQIRILRELKRRIELETGQKLPTGPSEKLPPTAQ 360

[Close]

BLAST of Lsi08G005630 vs. TrEMBL
Match: F6GY50_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_18s0072g00300 PE=4 SV=1)

HSP 1 Score: 440.7 bits (1132), Expect = 1.7e-120
Identity = 230/334 (68.86%), Postives = 260/334 (77.84%), Query Frame = 1


Query: 36  NFQFQRHFKLESSKNGGRETQR---------DCAFDEVAFEAERSRLDAQAVKSMAEASI 95
           +F F   F L  +  G R  Q+         D  FDE AFEAER  LDA+A +SMAEAS 
Sbjct: 23  SFSFSVPFSLNKNVGGSRNYQKLGSSRSSRNDAVFDEAAFEAERLTLDARARESMAEASK 82

Query: 96  RDTEGASDDDPKAWKWIIRKRVWDFMESQNIAANPRPVHHRIPNFLGAMEAANRLCDLEV 155
           R+TE     DPKAWKW+IRKRVWDFME++NIA NPRPVHHRIPNF+GA  AA +L  LE 
Sbjct: 83  RETE----TDPKAWKWVIRKRVWDFMETRNIAQNPRPVHHRIPNFVGAAAAAQKLGGLEA 142

Query: 156 FRDSQCVKVNPDSPQKGVRLLTLTGGKKLLTPQPRLRTGFFSIVESGMLTPATINEACTS 215
           F  ++CVKVNPDSPQK VR LTL+GGKKLLTPQPRLRTGFFS++ES MLTP+TINEACTS
Sbjct: 143 FGVAKCVKVNPDSPQKQVRFLTLSGGKKLLTPQPRLRTGFFSVLESSMLTPSTINEACTS 202

Query: 216 VGVAKYGKPIGLDEKIKVDLIIIGSVAVDPRTGARLGKGE---------------ISNS- 275
           VGVAKYG+PIGLDE+IKVDLI+IGSVAVDP+TGARLGKGE               I ++ 
Sbjct: 203 VGVAKYGRPIGLDEQIKVDLIVIGSVAVDPKTGARLGKGEGFAELEYGMLRYMGAIDDTT 262

Query: 276 ---VAVHDCQLVDDIPVEKLLVHDVPVDIVCTPTQVILTNTRIPKPQGIYWEMLSPEKLS 335
               +VHD QLVDDIPVEKLL+HDVPVDI+CTPTQVI TNT IPKPQGIYW+ LSPEKL 
Sbjct: 263 PIVTSVHDEQLVDDIPVEKLLIHDVPVDIICTPTQVIFTNTSIPKPQGIYWDKLSPEKLG 322

Query: 336 QVRILRELKRRIERETGKPLPCGPSEKLPPTAQR 342
           Q+RILRELK RIE ETG+ LPCGPSEKLPPTA+R
Sbjct: 323 QIRILRELKSRIEGETGQKLPCGPSEKLPPTAKR 352

[Close]

BLAST of Lsi08G005630 vs. TrEMBL
Match: A0A0D2QAU8_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_002G231100 PE=4 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 2.3e-120
Identity = 224/321 (69.78%), Postives = 258/321 (80.37%), Query Frame = 1


Query: 42  HFKLESSKNGGRETQRDCAFDEVAFEAERSRLDAQAVKSMAEAS--IRDTEGASDDDPKA 101
           +F+L+S KN   + +    FDE AFEAER RLDA+A +SMA+ S  + +T   ++ DPKA
Sbjct: 49  NFRLQSRKNSTSDAKE---FDESAFEAERLRLDAKARESMAQISKTMTETTAEAEADPKA 108

Query: 102 WKWIIRKRVWDFMESQNIAANPRPVHHRIPNFLGAMEAANRLCDLEVFRDSQCVKVNPDS 161
           WKW+IRKR+WD ME+QNIA NPRPVHHRIPNF+GA  AA +L +LE F  + CVKVNPDS
Sbjct: 109 WKWVIRKRIWDLMEAQNIAQNPRPVHHRIPNFVGASSAAKKLSELEAFVMANCVKVNPDS 168

Query: 162 PQKGVRLLTLTGGKKLLTPQPRLRTGFFSIVESGMLTPATINEACTSVGVAKYGKPIGLD 221
           PQK VR LTL+ GKKLLTPQPRLRTGFFSI+ES MLTP+TINEACTSVGVAKYG+PIG D
Sbjct: 169 PQKQVRFLTLSDGKKLLTPQPRLRTGFFSILESSMLTPSTINEACTSVGVAKYGRPIGFD 228

Query: 222 EKIKVDLIIIGSVAVDPRTGARLGKGE---------------ISNS----VAVHDCQLVD 281
           EKIKVD+I+IGSVAVDP+TGARLGKGE               I +S     +VHDCQ+VD
Sbjct: 229 EKIKVDVIVIGSVAVDPKTGARLGKGEGFAELEYGMLRYMGAIDDSTPVVTSVHDCQIVD 288

Query: 282 DIPVEKLLVHDVPVDIVCTPTQVILTNTRIPKPQGIYWEMLSPEKLSQVRILRELKRRIE 341
           DIPVEKLLVHDVPVDI+CTPTQVI TN  +PKPQGIYW+ LSPEKL Q+RILRELK RIE
Sbjct: 289 DIPVEKLLVHDVPVDIICTPTQVIFTNRTVPKPQGIYWDKLSPEKLGQIRILRELKSRIE 348

[Close]

BLAST of Lsi08G005630 vs. TrEMBL
Match: A0A0B0Q0E2_GOSAR (Methenyltetrahydrofolate synthase domain-containing OS=Gossypium arboreum GN=F383_14415 PE=4 SV=1)

HSP 1 Score: 438.3 bits (1126), Expect = 8.6e-120
Identity = 224/321 (69.78%), Postives = 257/321 (80.06%), Query Frame = 1


Query: 42  HFKLESSKNGGRETQRDCAFDEVAFEAERSRLDAQAVKSMAEAS--IRDTEGASDDDPKA 101
           +F+L+S KN   + +    FDE AFEAER RLDA+A +SMA+ S  + +T   ++ DPKA
Sbjct: 34  NFRLQSRKNSNSDAKE---FDESAFEAERLRLDAKARESMAQISKTMTETTAEAEADPKA 93

Query: 102 WKWIIRKRVWDFMESQNIAANPRPVHHRIPNFLGAMEAANRLCDLEVFRDSQCVKVNPDS 161
           WKW+IRKR+WD ME+QNIA NPRPVHHRIPNF+GA  AA +L +LE F  + CVKVNPDS
Sbjct: 94  WKWVIRKRIWDLMEAQNIAQNPRPVHHRIPNFVGASSAAKKLSELEAFVMANCVKVNPDS 153

Query: 162 PQKGVRLLTLTGGKKLLTPQPRLRTGFFSIVESGMLTPATINEACTSVGVAKYGKPIGLD 221
           PQK VR LTL+ GKKLLTPQPRLRTGFFSI+ES MLTP+TINEACTSVGVAKYG+PIGLD
Sbjct: 154 PQKQVRFLTLSDGKKLLTPQPRLRTGFFSILESSMLTPSTINEACTSVGVAKYGRPIGLD 213

Query: 222 EKIKVDLIIIGSVAVDPRTGARLGKGE---------------ISNS----VAVHDCQLVD 281
           EKIKVD+I+IGSVAVDP TGARLGKGE               I +S     +VHDCQ+VD
Sbjct: 214 EKIKVDVIVIGSVAVDPTTGARLGKGEGFAELEYGMLRYMGAIDDSTLVVTSVHDCQIVD 273

Query: 282 DIPVEKLLVHDVPVDIVCTPTQVILTNTRIPKPQGIYWEMLSPEKLSQVRILRELKRRIE 341
           DIPVEKLLVHDVPVDI+CTPTQVI TN  IPKPQGIYW+ LSPEKL Q+RILRELK RIE
Sbjct: 274 DIPVEKLLVHDVPVDIICTPTQVIFTNRTIPKPQGIYWDKLSPEKLGQIRILRELKSRIE 333

[Close]

BLAST of Lsi08G005630 vs. TAIR10
Match: AT1G76730.1 (AT1G76730.1 NagB/RpiA/CoA transferase-like superfamily protein)

HSP 1 Score: 407.1 bits (1045), Expect = 1.1e-113
Identity = 203/312 (65.06%), Postives = 243/312 (77.88%), Query Frame = 1


Query: 52  GRETQRDCAFDEVAFEAERSRLDAQAVKSMAEASIRDTEGASDDDPKAWKWIIRKRVWDF 111
           G E     AFD VA+EA+R  LDA A++ MAE + ++ E   D DPKAWKW+IRK++WD 
Sbjct: 43  GSENGDGVAFDAVAYEADRLSLDAAAMEDMAETAKKELESDPDSDPKAWKWVIRKKMWDL 102

Query: 112 MESQNIAANPRPVHHRIPNFLGAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLTG 171
           ME++N A +PRPVHHRIPNF+GA  AA +L +L+ FR +  VKVNPDSPQK +R LTL+G
Sbjct: 103 MEARNYAMSPRPVHHRIPNFVGASAAARKLAELDAFRMAMVVKVNPDSPQKQIRFLTLSG 162

Query: 172 GKKLLTPQPRLRTGFFSIVESGMLTPATINEACTSVGVAKYGKPIGLDEKIKVDLIIIGS 231
            KKLLTPQPRLRTGFFS++ES +L P TI EACTSVGVAKYG+ IGLDEKIKVDLI+IGS
Sbjct: 163 EKKLLTPQPRLRTGFFSVLESDLLKPETIMEACTSVGVAKYGRAIGLDEKIKVDLIVIGS 222

Query: 232 VAVDPRTGARLGKGE---------------ISNS----VAVHDCQLVDDIPVEKLLVHDV 291
           VAV+P+TGARLGKGE               I +S      VHDCQLVDDIP+EKL +HDV
Sbjct: 223 VAVNPQTGARLGKGEGFAELEYGMLRYMGAIDDSTPVVTTVHDCQLVDDIPLEKLAIHDV 282

Query: 292 PVDIVCTPTQVILTNTRIPKPQGIYWEMLSPEKLSQVRILRELKRRIERETGKPLPCGPS 345
           PVDI+CTPT+VI TNT IPKPQGIYW+ LSPEKL Q+RILRELK R+E++TG+ LP GPS
Sbjct: 283 PVDIICTPTRVIFTNTPIPKPQGIYWDKLSPEKLRQIRILRELKNRLEKKTGRKLPTGPS 342

[Close]

BLAST of Lsi08G005630 vs. NCBI nr
Match: gi|778710781|ref|XP_004140963.2| (PREDICTED: LOW QUALITY PROTEIN: 5-formyltetrahydrofolate cyclo-ligase-like protein COG0212 [Cucumis sativus])

HSP 1 Score: 599.0 bits (1543), Expect = 5.5e-168
Identity = 311/373 (83.38%), Postives = 331/373 (88.74%), Query Frame = 1


Query: 1   MDSNLLQLSHTLSLKSTFLNAIRRSPSISSISIRRNFQFQR-HFKLESSKNGGRETQRDC 60
           MDS L+QLS  LSL+ST LNAIR  PSISSIS+  N  FQR HFKL+S+KNGG +TQRD 
Sbjct: 1   MDSILIQLSQPLSLRSTLLNAIRTPPSISSISLGGNLHFQRRHFKLDSTKNGGTDTQRDP 60

Query: 61  AFDEVAFEAERSRLDAQAVKSMAEASIRDTEGASDDDPKAWKWIIRKRVWDFMESQNIAA 120
            FDE AFEAERSRLDAQA KSMAEASIRDTEGASDDDPKAWKW+IRKR+WDFMESQN+AA
Sbjct: 61  PFDEPAFEAERSRLDAQARKSMAEASIRDTEGASDDDPKAWKWVIRKRIWDFMESQNVAA 120

Query: 121 NPRPVHHRIPNFLGAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLTGGKKLLTPQ 180
           NPRPVHHRIPNF+GAMEAANRLCDLEVFR+SQCVKVNPDSPQKGVRLLTLTGGKKLLTPQ
Sbjct: 121 NPRPVHHRIPNFIGAMEAANRLCDLEVFRNSQCVKVNPDSPQKGVRLLTLTGGKKLLTPQ 180

Query: 181 PRLRTGFFSIVESGMLTPATINEACTSVGVAKYGKPIGLDEKIKVDLIIIGSVAVDPRTG 240
           PRLRTGFFSIVESGMLTPATI EACTSVGVAKYGKPIGLDEKIKVDLI+IGSVAVDP+TG
Sbjct: 181 PRLRTGFFSIVESGMLTPATIKEACTSVGVAKYGKPIGLDEKIKVDLIVIGSVAVDPKTG 240

Query: 241 ARLGKGE---------------ISNS----VAVHDCQLVDDIPVEKLLVHDVPVDIVCTP 300
           ARLGKGE               I +S     +VHDCQLVDDIPV+KLL+HDVPV IVCTP
Sbjct: 241 ARLGKGEGFAELEYGMLRYMGAIDDSTLIVTSVHDCQLVDDIPVQKLLIHDVPVXIVCTP 300

Query: 301 TQVILTNTRIPKPQGIYWEMLSPEKLSQVRILRELKRRIERETGKPLPCGPSEKLPPTAQ 354
           TQVILTNT+IPKPQGIYWEMLSPEKLSQ+RILRELKRRIERETGK LPCGPSEKLPPTAQ
Sbjct: 301 TQVILTNTKIPKPQGIYWEMLSPEKLSQIRILRELKRRIERETGKLLPCGPSEKLPPTAQ 360

[Close]

BLAST of Lsi08G005630 vs. NCBI nr
Match: gi|659081769|ref|XP_008441505.1| (PREDICTED: 5-formyltetrahydrofolate cyclo-ligase-like protein COG0212 isoform X2 [Cucumis melo])

HSP 1 Score: 598.2 bits (1541), Expect = 9.3e-168
Identity = 310/374 (82.89%), Postives = 331/374 (88.50%), Query Frame = 1


Query: 1   MDSNLLQLSHTLSLKSTFLNAIRRSPSISSISIRRNFQF--QRHFKLESSKNGGRETQRD 60
           MDS L+QLS  LSLKST LNAIR  PSISSIS+R +     +RHFKLES+KNGG +TQRD
Sbjct: 1   MDSILMQLSQPLSLKSTLLNAIRTPPSISSISLRGSLHLIQRRHFKLESTKNGGTDTQRD 60

Query: 61  CAFDEVAFEAERSRLDAQAVKSMAEASIRDTEGASDDDPKAWKWIIRKRVWDFMESQNIA 120
             FDE AFEAERSRLDAQA KSMAEAS+RDTEGASDDDPKAWKW+IRKR+WDFMESQN+A
Sbjct: 61  LPFDEAAFEAERSRLDAQARKSMAEASMRDTEGASDDDPKAWKWVIRKRIWDFMESQNVA 120

Query: 121 ANPRPVHHRIPNFLGAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLTGGKKLLTP 180
           ANPRPVHHRIPNF+GAMEAANRLC+LEVF DSQCVKVNPDSPQKGVRLLTLTGGKKLLTP
Sbjct: 121 ANPRPVHHRIPNFIGAMEAANRLCELEVFSDSQCVKVNPDSPQKGVRLLTLTGGKKLLTP 180

Query: 181 QPRLRTGFFSIVESGMLTPATINEACTSVGVAKYGKPIGLDEKIKVDLIIIGSVAVDPRT 240
           QPRLRTGFFSIVESGMLTPATI EACTSVGVAKYGKPIGLDEKIKVDLI+IGSVAVDP+T
Sbjct: 181 QPRLRTGFFSIVESGMLTPATIKEACTSVGVAKYGKPIGLDEKIKVDLIVIGSVAVDPKT 240

Query: 241 GARLGKGE---------------ISNS----VAVHDCQLVDDIPVEKLLVHDVPVDIVCT 300
           GARLGKGE               I +S     +VHDCQLVDDIPV+KLLVHDVPVDIVCT
Sbjct: 241 GARLGKGEGFAELEYGMLRYMGAIDDSTLIVTSVHDCQLVDDIPVQKLLVHDVPVDIVCT 300

Query: 301 PTQVILTNTRIPKPQGIYWEMLSPEKLSQVRILRELKRRIERETGKPLPCGPSEKLPPTA 354
           PTQVILTNT+IPKPQGIYWEMLSPEKLSQ+RILRELKRRIERETGK LPCGPSEKLPPTA
Sbjct: 301 PTQVILTNTKIPKPQGIYWEMLSPEKLSQIRILRELKRRIERETGKLLPCGPSEKLPPTA 360

[Close]

BLAST of Lsi08G005630 vs. NCBI nr
Match: gi|659081767|ref|XP_008441504.1| (PREDICTED: 5-formyltetrahydrofolate cyclo-ligase-like protein COG0212 isoform X1 [Cucumis melo])

HSP 1 Score: 588.6 bits (1516), Expect = 7.4e-165
Identity = 310/388 (79.90%), Postives = 331/388 (85.31%), Query Frame = 1


Query: 1   MDSNLLQLSHTLSLKSTFLNAIRRSPSISSISIRRNFQF--QRHFKLESSKNGGRETQRD 60
           MDS L+QLS  LSLKST LNAIR  PSISSIS+R +     +RHFKLES+KNGG +TQRD
Sbjct: 1   MDSILMQLSQPLSLKSTLLNAIRTPPSISSISLRGSLHLIQRRHFKLESTKNGGTDTQRD 60

Query: 61  CAFDEVAFEAERSRLDAQAVKSMAEASIRDTEGASDDDPKAWKWIIRKRVWDFMESQNIA 120
             FDE AFEAERSRLDAQA KSMAEAS+RDTEGASDDDPKAWKW+IRKR+WDFMESQN+A
Sbjct: 61  LPFDEAAFEAERSRLDAQARKSMAEASMRDTEGASDDDPKAWKWVIRKRIWDFMESQNVA 120

Query: 121 ANPRPVHHRIPNFLGAMEAANR--------------LCDLEVFRDSQCVKVNPDSPQKGV 180
           ANPRPVHHRIPNF+GAMEAANR              LC+LEVF DSQCVKVNPDSPQKGV
Sbjct: 121 ANPRPVHHRIPNFIGAMEAANRVSYPIPADEMELYQLCELEVFSDSQCVKVNPDSPQKGV 180

Query: 181 RLLTLTGGKKLLTPQPRLRTGFFSIVESGMLTPATINEACTSVGVAKYGKPIGLDEKIKV 240
           RLLTLTGGKKLLTPQPRLRTGFFSIVESGMLTPATI EACTSVGVAKYGKPIGLDEKIKV
Sbjct: 181 RLLTLTGGKKLLTPQPRLRTGFFSIVESGMLTPATIKEACTSVGVAKYGKPIGLDEKIKV 240

Query: 241 DLIIIGSVAVDPRTGARLGKGE---------------ISNS----VAVHDCQLVDDIPVE 300
           DLI+IGSVAVDP+TGARLGKGE               I +S     +VHDCQLVDDIPV+
Sbjct: 241 DLIVIGSVAVDPKTGARLGKGEGFAELEYGMLRYMGAIDDSTLIVTSVHDCQLVDDIPVQ 300

Query: 301 KLLVHDVPVDIVCTPTQVILTNTRIPKPQGIYWEMLSPEKLSQVRILRELKRRIERETGK 354
           KLLVHDVPVDIVCTPTQVILTNT+IPKPQGIYWEMLSPEKLSQ+RILRELKRRIERETGK
Sbjct: 301 KLLVHDVPVDIVCTPTQVILTNTKIPKPQGIYWEMLSPEKLSQIRILRELKRRIERETGK 360

[Close]

BLAST of Lsi08G005630 vs. NCBI nr
Match: gi|566172564|ref|XP_002306710.2| (hypothetical protein POPTR_0005s21670g [Populus trichocarpa])

HSP 1 Score: 453.0 bits (1164), Expect = 4.8e-124
Identity = 243/367 (66.21%), Postives = 277/367 (75.48%), Query Frame = 1


Query: 1   MDSNLLQLSHTLSLKSTFL-NAIRRSPSISSISIRRNFQ---FQRHFKLESSKNGGRETQ 60
           MDS L +++  L L  T L N    SPS+     RR      F  + KL SSK    +  
Sbjct: 1   MDSTLTRIAAPLPLTHTLLFNYPHSSPSLICFFSRRTHSKRLFNSNCKLGSSKRNAHDD- 60

Query: 61  RDCAFDEVAFEAERSRLDAQAVKSMAEASIRDTEGASDDDPKAWKWIIRKRVWDFMESQN 120
            +   DE A+EAER R DAQA  SMAE S R+ E +S+DDPKAWKW+IRKR+WD ME++N
Sbjct: 61  -NVVLDEKAYEAERLRFDAQARDSMAETSKREMETSSEDDPKAWKWVIRKRIWDLMEARN 120

Query: 121 IAANPRPVHHRIPNFLGAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLTGGKKLL 180
           IA NPRPVHHRIPNF+GA  AA  L  LEVF ++ CVKVNPDSPQK VR LTL+GGKKLL
Sbjct: 121 IAQNPRPVHHRIPNFVGASAAAKMLSGLEVFLEASCVKVNPDSPQKQVRFLTLSGGKKLL 180

Query: 181 TPQPRLRTGFFSIVESGMLTPATINEACTSVGVAKYGKPIGLDEKIKVDLIIIGSVAVDP 240
           TPQPRLRTGFFSI+ES ML+P+TINEACTSVGVAK G+PIGLDEKIKVDLI+IGSVAVDP
Sbjct: 181 TPQPRLRTGFFSILESHMLSPSTINEACTSVGVAKNGRPIGLDEKIKVDLIVIGSVAVDP 240

Query: 241 RTGARLGKGE---------------ISNS----VAVHDCQLVDDIPVEKLLVHDVPVDIV 300
           +TGARLGKGE               I +S     +VHDCQLV+DIPVEKLL+HDVPVDI+
Sbjct: 241 KTGARLGKGEGFAELEYGMLRYMGAIDDSTPVVTSVHDCQLVEDIPVEKLLIHDVPVDII 300

Query: 301 CTPTQVILTNTRIPKPQGIYWEMLSPEKLSQVRILRELKRRIERETGKPLPCGPSEKLPP 345
           CTP QVI TNT IPKPQGIYW+ LSPEKL+Q+RILRELK RIERETG+ LPCGPSEKLPP
Sbjct: 301 CTPDQVIFTNTNIPKPQGIYWDKLSPEKLAQIRILRELKSRIERETGQKLPCGPSEKLPP 360

[Close]

BLAST of Lsi08G005630 vs. NCBI nr
Match: gi|743901865|ref|XP_011044259.1| (PREDICTED: 5-formyltetrahydrofolate cyclo-ligase-like protein COG0212 [Populus euphratica])

HSP 1 Score: 451.8 bits (1161), Expect = 1.1e-123
Identity = 243/367 (66.21%), Postives = 277/367 (75.48%), Query Frame = 1


Query: 1   MDSNLLQLSHTLSLKSTFL-NAIRRSPSISSISIRRNFQ---FQRHFKLESSKNGGRETQ 60
           MDS L +++    L  T L N    SPS+     RR      F  + KLESSK    +  
Sbjct: 1   MDSTLTRIAAPFPLTHTLLYNYPHSSPSLICFFSRRTHSKRLFNSNCKLESSKRNAHDD- 60

Query: 61  RDCAFDEVAFEAERSRLDAQAVKSMAEASIRDTEGASDDDPKAWKWIIRKRVWDFMESQN 120
            +   DE A+EAER RLDAQA  SMAE S R+ E +S+DDPKA KW+IR R+WD ME++N
Sbjct: 61  -NAVLDEKAYEAERLRLDAQARDSMAETSKREMETSSEDDPKARKWVIRNRIWDLMEARN 120

Query: 121 IAANPRPVHHRIPNFLGAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLTGGKKLL 180
           IA NPRPVHHRIPNF+GA  AA  L  LEVF D+ CVKVNPDSPQK VR LTL+GGKKLL
Sbjct: 121 IAQNPRPVHHRIPNFVGASAAAKMLSGLEVFLDASCVKVNPDSPQKQVRFLTLSGGKKLL 180

Query: 181 TPQPRLRTGFFSIVESGMLTPATINEACTSVGVAKYGKPIGLDEKIKVDLIIIGSVAVDP 240
           TPQPRLRTGFFSI+ES ML+P+TINEACTSVGVAK+G+PIGLDEKIKVDLI+IGSVAVDP
Sbjct: 181 TPQPRLRTGFFSILESHMLSPSTINEACTSVGVAKHGRPIGLDEKIKVDLIVIGSVAVDP 240

Query: 241 RTGARLGKGE---------------ISNS----VAVHDCQLVDDIPVEKLLVHDVPVDIV 300
           +TGARLGKGE               I +S     +VHDCQLV+DIPVEKLL+HDVPVDI+
Sbjct: 241 KTGARLGKGEGFAELEYGMLRYMGAIDDSTPVVTSVHDCQLVEDIPVEKLLIHDVPVDII 300

Query: 301 CTPTQVILTNTRIPKPQGIYWEMLSPEKLSQVRILRELKRRIERETGKPLPCGPSEKLPP 345
           CTP QVI TNT IPKPQGIYW+ LSPEKL+Q+RILRELK RIERETG+ LPCGPSEKLPP
Sbjct: 301 CTPDQVIFTNTNIPKPQGIYWDKLSPEKLAQIRILRELKSRIERETGQKLPCGPSEKLPP 360

The following BLAST results are available for this feature:

BLAST of Lsi08G005630 vs. Swiss-Prot


Match Name	E-value	Identity	Description
5FCLL_ARATH	1.9e-112	65.06	5-formyltetrahydrofolate cyclo-ligase-like protein COG0212 OS=Arabidopsis thalia...	[more]
MTHSD_DANRE	6.5e-52	45.31	Methenyltetrahydrofolate synthase domain-containing protein OS=Danio rerio GN=mt...	[more]
MTHSD_BOVIN	2.7e-50	44.44	Methenyltetrahydrofolate synthase domain-containing protein OS=Bos taurus GN=MTH...	[more]
MTHSD_XENLA	7.9e-50	43.48	Methenyltetrahydrofolate synthase domain-containing protein OS=Xenopus laevis GN...	[more]
MTHSD_HUMAN	6.7e-49	44.49	Methenyltetrahydrofolate synthase domain-containing protein OS=Homo sapiens GN=M...	[more]

BLAST of Lsi08G005630 vs. TrEMBL


Match Name	E-value	Identity	Description
B9H7U6_POPTR	3.4e-124	66.21	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0005s21670g PE=4 SV=2	[more]
B9SE88_RICCO	1.0e-120	64.84	Catalytic, putative OS=Ricinus communis GN=RCOM_0959760 PE=4 SV=1	[more]
F6GY50_VITVI	1.7e-120	68.86	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_18s0072g00300 PE=4 SV=...	[more]
A0A0D2QAU8_GOSRA	2.3e-120	69.78	Uncharacterized protein OS=Gossypium raimondii GN=B456_002G231100 PE=4 SV=1	[more]
A0A0B0Q0E2_GOSAR	8.6e-120	69.78	Methenyltetrahydrofolate synthase domain-containing OS=Gossypium arboreum GN=F38...	[more]

BLAST of Lsi08G005630 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G76730.1	1.1e-113	65.06	NagB/RpiA/CoA transferase-like superfamily protein	[more]

BLAST of Lsi08G005630 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778710781\|ref\|XP_004140963.2\|	5.5e-168	83.38	PREDICTED: LOW QUALITY PROTEIN: 5-formyltetrahydrofolate cyclo-ligase-like prote...	[more]
gi\|659081769\|ref\|XP_008441505.1\|	9.3e-168	82.89	PREDICTED: 5-formyltetrahydrofolate cyclo-ligase-like protein COG0212 isoform X2...	[more]
gi\|659081767\|ref\|XP_008441504.1\|	7.4e-165	79.90	PREDICTED: 5-formyltetrahydrofolate cyclo-ligase-like protein COG0212 isoform X1...	[more]
gi\|566172564\|ref\|XP_002306710.2\|	4.8e-124	66.21	hypothetical protein POPTR_0005s21670g [Populus trichocarpa]	[more]
gi\|743901865\|ref\|XP_011044259.1\|	1.1e-123	66.21	PREDICTED: 5-formyltetrahydrofolate cyclo-ligase-like protein COG0212 [Populus e...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR024185	FTHF_cligase-like_sf
IPR002698	FTHF_cligase

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
biological_process	GO:0044723	single-organism carbohydrate metabolic process
biological_process	GO:0008298	intracellular mRNA localization
biological_process	GO:0019252	starch biosynthetic process
biological_process	GO:0000023	maltose metabolic process
biological_process	GO:0016117	carotenoid biosynthetic process
biological_process	GO:0044763	single-organism cellular process
biological_process	GO:0044711	single-organism biosynthetic process
biological_process	GO:1901576	organic substance biosynthetic process
biological_process	GO:0044262	cellular carbohydrate metabolic process
biological_process	GO:0044249	cellular biosynthetic process
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0016874	ligase activity
molecular_function	GO:0003674	molecular_function
molecular_function	GO:0003723	RNA binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G005630.1	Lsi08G005630.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002698	5-formyltetrahydrofolate cyclo-ligase	PFAM	PF01812	5-FTHF_cyc-lig	coord: 101..246 score: 7.3
IPR024185	5-formyltetrahydrofolate cyclo-ligase-like domain	GENE3D	G3DSA:3.40.50.10420		coord: 252..298 score: 4.8E-8coord: 100..246 score: 1.1
None	No IPR available	PANTHER	PTHR13017	5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE-RELATED	coord: 70..341 score: 1.7E
None	No IPR available	unknown	SSF100950	NagB/RpiA/CoA transferase-like	coord: 100..299 score: 9.77

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi08G005630	Csa6G055960	Cucumber (Chinese Long) v2	culsiB478
Lsi08G005630	CSPI06G04780	Wild cucumber (PI 183967)	cpilsiB487
Lsi08G005630	Cucsa.363800	Cucumber (Gy14) v1	cgylsiB588
Lsi08G005630	CmaCh15G005880	Cucurbita maxima (Rimu)	cmalsiB294
Lsi08G005630	CmoCh15G005940	Cucurbita moschata (Rifu)	cmolsiB282
Lsi08G005630	Cla013809	Watermelon (97103) v1	lsiwmB466
Lsi08G005630	MELO3C008332	Melon (DHL92) v3.5.1	lsimeB475
Lsi08G005630	ClCG08G007080	Watermelon (Charleston Gray)	lsiwcgB439
Lsi08G005630	MELO3C008332.2	Melon (DHL92) v3.6.1	lsimedB494
Lsi08G005630	Cla97C08G149790	Watermelon (97103) v2	lsiwmbB445
Lsi08G005630	Bhi04G001355	Wax gourd	lsiwgoB602
Lsi08G005630	Cp4.1LG13g04030	Cucurbita pepo (Zucchini)	cpelsiB158
Lsi08G005630	CsGy6G004730	Cucumber (Gy14) v2	cgyblsiB444
Lsi08G005630	Carg01610	Silver-seed gourd	carlsiB758
Lsi08G005630	CsaV3_6G005110	Cucumber (Chinese Long) v3	cuclsiB509

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi08G005630	Bottle gourd (USVL1VR-Ls)	lsilsiB122
Lsi08G005630	Cucurbita maxima (Rimu)	cmalsiB703
Lsi08G005630	Cucurbita moschata (Rifu)	cmolsiB697
Lsi08G005630	Cucurbita pepo (Zucchini)	cpelsiB382
Lsi08G005630	Silver-seed gourd	carlsiB059