Lsi08G000170 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi08G000170
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionProtein NRT1/ PTR FAMILY 5.10
Locationchr08 : 398045 .. 404062 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATATGGCTGATGAATCCCGAAGGCCGCTGATATTAAACGACGACGTCGTACCGGGGTCCGTGGACCACCGAGAGCGTCCGAGCCGGCGATCAGAGTCTGGGCGGTGGAGGGCGGCGGGGTTGATAATTGGAGTTGAAATAGCGGAGCGGTTGGCATTCTTCGGGATAAGCACCAATCTGGTAAGCTACTTGACAGTGGAGATGGGGCAGTCCATGGCCGCCGCCGCCAAGAATGTAAATCTCTGGATCGGAACCGCCTCTTTGCTCCCATTGCTTGCCGCCTCTTTCGCCGATTCCTTCCTCGGACGCTACCTTACTATTCTTCTCGCCTCCGCTCTTTACATCTTGGTTAACCTTCTTTTTCCTTTTCTTTTTCTCATTCTCATTCTTTTAAACATCATCATAATAAACAAATTATGTAAAACACTTCATTTTTTCTTAAAATATATAAGTTCTGACTCTTTTTAATTTTCTAAATCTATATACTTAACTGAGGGGGCAATTTCTTTTATGACATAAACAAAAGGTGAAATAGATAACAGTTAGAGAGGGGGAGAGGAAAATATAGGAATAATAAAGAAGTCTAACGTCATAAATTAAATGGGGAGACAGAGGAAATGGAAGCGGTTGAATCTTTAGAATTAAGGTAATATTGCACTTTTGAATGTTTAGAGTTATTAACGGTTTAGGCATTTTAAGAATAATAATTGTGTATATGATAATATTTTTAAAAATTTGCAAATATAGTAAAATCTATTCGTGATTAGCTCTATTACTACTAGACTTCTACTAGTTATGATCTATCATTGATAGATTTTGAGAATTGGATCTAAATTTTGTCATATCTACAAATTCTTTTGGAAATTTTCACCAATAAAAAAAAGGAAACTATTTAAAAAAAATAGAAAAACAAAATAGCAAAAACATCACTAATAGACAGTTCTATCCGCATCTATCATTGATAGATTTCTATTATTTCTATCATTGATAGATTTCTATCATCATCTATCAAACTATCTAAAAATTTTGCTATTTTGTGTGGATGGTTTCCTTTATTTTTCTATTTTTAAAAACTCCCCAATTTTTTTTTTTTTTTTTACCATCTACATTGAGCCTCATCACTATATTTGCAACTAGTCTTAAATTTTAATGACTAACCATTTAGTATTTATACTTGTAACAATTTTTTTCTTTATTATAAAAAATCTTATGAAAACTAATGGTTTTCTTTTTTTTTTTTTTTTCCTTTGAGAAAAAATTACTTTATTAAGCCACATGGGCATTAGCATCAATTGCAGCAATGATTAACGGTTAATTTACCACGATTTAGTTTTATAGTTTATAAATAAATGTGATTTTCCATGGGTAATCTTGATAATATATATTTTCACAATACAAAGTTTAAATCATTACAAATGGTACAATGGTAAAGTACAAGCGCACTAAGTTCTTACTTATGGACTAAATTGTTTCCATTTGAAAGTATCCTTAACTAACTACTGTTAAGTTGAGAATTAAATTATAATTCTAAAAAAAAAAATTGAGAATTAAATTATTATTATTATTATTATTATTATTAGTTTTTTTTATAAAAAAAAGCCTAATAGGTTTAAAAAAATTAGAGGAAAAATTTCAAATTATTTACCAATATAGAAATTAGCTATAGATTTTTATTGTTATCTATCGCGATACAATTCTATCACCGATAGACCGTAATTTTTTTTAGATTTATAAATAGTTTGACTCATTTTTCTATATTTGAAAACGGTCTATGAGTTTAAGTTTTGTTTTTTTTAATGAATGAACTGAAATGCAGGGACTTGGCCTACTGACACTCTCAGCTACGCTCCGGGGATCTGGCTCTGCTGCCACGGGAGCTTCATCTCAGCCTGTTCCTGATGCTGATGTCGTGTTGTTCTTCAGCGCTTTGTACTTGGTGGCATTTGCACAAGGTGGACACAAGCCTTGTGTTCAAGCATTTGGTTGTGATCAATTTGATGGAGAAGATCCTCAAGAATGCATAGCCAAATGCTCTTTCTTCAACTGGTGGTACTTTGCCACTACTTTTGGATCCTTCATTGCTCTAATCATTTTAAACTACATTCAAGATAACCTCGGTTGGGGTCTTGGCTTCGGCATTCCCTGCATCTCCTCCGTAGCTGCTCTCGTTGTGTTCTTGCTCGGGACTCGGACTTATCGTTTCGTTACAGTGGCCAACAATGAGGAGAAAACATTCATGAGAATTGGTCGGGTTTTTGTCAATGCGGCTCGAAACTGGAGAACGACATCGTCGGACATTGCTATTTTGGAGGAAGGTCAAGATGCAATGCTTTATCAAAGATATGGACAATTGAGGTAAGCTAGTTGAGATTTCCCTTTCTTATTATTTTTCTTTCATCCTATATATTGATGTTGATTAAGCAATGTTTTTCCCCTTTAATTTATTTCATAAATCTTCTTTTGTGTTTACAATTAAATTGTTTGTTTTGTTTTTTTTCCGTAATTTATTTTTATAATTTTATTTCAATTCTAATAAAAGAGATGTGGTTATGCATATGGTGTCCTTGACCAATGGGAAGCAGCCAATTATGTAGAATATGGACAATTCTATATTTGTCTATTCAATGTCTCTTATTGTGTAGGAACTTCTAATGGATTTTAGGAAGCAACAATATATGATGGCTAATCTATTCGGAACACAAAATTTTTTTATTTAATATTAATATTATTATTATTATTTTGACAATAAGTGGGTTAGGGGAATCAAACCCGACTAGTACTTTGAGCTAGGCTCATTTTGGTAAATTTGGACTGATTTTGTTAATTTAAAACGTCGTATTTCATTATTCCTTTTGAAATTTGGACAACATTTCTAATGGATTTCTAAAGGGTTAAAATGTTGACTCAATTTCATAGAAATGTATTGATGAATATTTCTCCTAAAAGTTTAATTACAAAAAAAAAAAAAAAAATGAAGGAAAATTAATAAATAAACATTTTGTTTTTTTTAAATAAGTTAAAATATTGTTTATTTATATTTAATTCATATTAGTGACATATAAATTTGCTTATTTTGTATGTTTTCATTATTTTTTTTTTTCATTGAATAAGATATTGATTCATTCCACGTTGAATTTATGGGTATATGACAATGCCGATGAAAAATGTTTGACATGTTAACAAAAACATTAACACACTTTTTACTACATAGCAAAAGAAAAAATACTAGAGAAACTAGAGAACTCATGTATATGAAAGAAAATGAAGATTAAAATTAGGTTAAATAACAAGTTTAAGTTCGTAAGTTTGAGATTTGTTTTTATTTGATAGGTAGAAGAGGTGTTATAGGCTTCATTTAAAAAAAATTACTAATAAGTTTTTTTAATTGTGTCTAACATGTTCTTGAAAAAATTGAATACAAATTTTATTTTGGGAAAAGAGAAGACAAGACAATTTAACCCATAGCTTCTCATTTCCAGCCTTAGGAATAAGGGGTCGGGGGGATTCCCCATACCTAACTCTGTCTCCCAATTTGAGGGGAACAGGGGGTTCTGTCGGGGAATCACGTCGGTGATTTCCATCTCTGTTTTTATGGAAAATTTTTCGTTATTTCTCAATTTTTTTAAAATTTTTTTTATTAGCTATTATTTTTTAATCTATACTTTCGTTTAATAATACTTTTTAATTTTAGATTGGAGTATCTTTTTTCTGTGTAGATTACAAACCTTTGTGAAAAAAAATTAATAAACACAAATGCATACCTTTGAAAAAAATACATTAATAATAAAATGTGTTATTTTTAAATATAGAAAAATGAGTCAAACTATTTTTATATTTCACTATCTATCAGCGATAGAGTTCTATCACGATCTATCGTTTGAGCGATAGACAACAATAGAAGTCTATCAAGTGATAGATGTCTATCGCGGTCTATCGTTAATAGATAGTAATTTATTTTTTATTTTTCATAAAGTAAAATTAAGTAAGTGATATAATGTCATAGTTGTCTTCTTTCTAAGTAGAAAAATATATTAGTACAATAGAATGTAAAACTATAATAATATAATCAATAAGAAAACTATATCTGGTCATCTTCTTGTCCTTTAATCCCAATAACAAACTAAAACTAATAAAGAAAAATAGTTGGGGAGACCATTCTTCGTGAGTTTTTTTCCCTACCTTACTCTTCTTTCTTTGTGAGAATAAAGGAGGAAAATATGGCTGAAAATGATCCAAAACTCAAAAACTTCTAAAACAAATGTCCTCTTTTTAAAAGAACATGTTTTTTATTCTTTGTTGAGAAGTGAGATTATCATTTTATCCTTATAGTACAAAACATTAAATATCATAAATGATTTAAGTTCCGATGCCACTCAAAAGGCAAGATGTTTAAAATTTTTCTCAATTTATAAACATGTTTGGAAAGAACACGAAAACAATGTATGAAAAATGAGTGGTCATTTACCTAAAACGGATTTGTATCAATGAAGATATGATAGATCAAAACAAAATTACTTCGTGGATCGCGCAATAAAAAATGCGTCATTGCGCATACCTCTCGTTGTGCGCACTCTTATACTTCTCGCTTTGCGTTTTTTTTTGTCATAGCTCTCTTGATACAACTTCGTTTTAGCTAGAGGATCACTCCTTCTTATACATTTTTTCATGTTATTTTTAACCATGCTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAGAATCGTGAATTTAAATGGCCTTAAAAAGTTTCAACATAGAGTAATTTGGCTTTTGGGTATTGAAGCTAAAGATTTTTTTTTTAATTTATAAGCATGGTGGGAAAGAATACGAAAAAAAAACGTTAGAAAACATATACACGTGGTCATTTTTAGCAAAAGTCAAGTTGTATCAAAAGAGATATGAGAAAAGGTTTAAAAACATGTTTCTTTGTTTTTTTTTTTTTTTTTTTAAAGAAAATGAGGTCATTTGTAAAAAAGTTTTAAAAAGTTTTTGGGTTTTAGATTACTTTGAACGATTTTCCACCTCTTAGAAAAAGAAATACATCATATTATAAGATATGTGAGATCTTCAAAAGAGGATAGAGGTTGATATCGATAGTAAAAGTTTTATGTCATTTTCATACTACAGTTTTAATGTGGTGTATTTTGAATGTAACTGAGTGAGTGTTTTAAGTTGACAGGTTCCTTAACAAAGCCTTGGTTGCTCGAATGGACTCGAATAAAGATTTGAAAACGTGTAACATAGTGGAGGTTGAAGAAGCTAAGGGTATCCTAAGGCTGATCCCCATTTGGACAGCAAGCTTGGCATATGCCATTGTTTTATCACAATGCTCTACCTTCTTCGTCAAGCAAGCTTCCACCATGGACAGATCAATCTCACCAACCTTCAAAATACCCGCTGCTACAATCCAATGCTTTGCCCTCATTGCTGTTCTCCTCTTCCTTCCAATCTATGACCAACTCCTCGTTCCCATCGCCAGAATATTCACCCTCAAACCCTCCGGAATCTCCATGCTCCAAAGAATTGGAGTTGGGATGTTCATATCCACTCTTTCAATGGTTGTTGCCGCTCTTGTTGAATGTAAAAGGCTAGCCGTCGCTCGAGAACATGGCTTAACTCATAACCCTAACGCTACGATCCCTATCACCATCTGGTGGCTAACTCCACAGCTCATCCTCCTCGGAGTTTCTAGCGTTTTCACGATGGTAGGTTTGCAGGAGTTCTTCTACGACCAAGTTTTGATCGAGCTGAAGAGTGTGGGACTTGCACTGTATCTGAGCATCTTTGGAGTGGGGAACTTGCTTAGCGGTGTTCTTGTATCCATCATTGAAGACACAACTGGTCGACATGGAAGAGGTGGCTGGCTTGCTAACAACATAAATAATGCACATCTTGATTACTTTTATTGGCTTCTTGCTGGCATTGGTGAGGTAGGATTACTTGCATATATGTATTTTGCTAACTCTTATGTATATAAATACAACGTCGAAAGATCTAGAGATTAA

mRNA sequence

ATGGATATGGCTGATGAATCCCGAAGGCCGCTGATATTAAACGACGACGTCGTACCGGGGTCCGTGGACCACCGAGAGCGTCCGAGCCGGCGATCAGAGTCTGGGCGGTGGAGGGCGGCGGGGTTGATAATTGGAGTTGAAATAGCGGAGCGGTTGGCATTCTTCGGGATAAGCACCAATCTGGTAAGCTACTTGACAGTGGAGATGGGGCAGTCCATGGCCGCCGCCGCCAAGAATGTAAATCTCTGGATCGGAACCGCCTCTTTGCTCCCATTGCTTGCCGCCTCTTTCGCCGATTCCTTCCTCGGACGCTACCTTACTATTCTTCTCGCCTCCGCTCTTTACATCTTGGTTAACCTTCTTTTTCCTTTTCTTTTTCTCATTCTCATTCTTTTAAACATCATCATAATAAACAAATTATCTACGCTCCGGGGATCTGGCTCTGCTGCCACGGGAGCTTCATCTCAGCCTGTTCCTGATGCTGATGTCGTGTTGTTCTTCAGCGCTTTGTACTTGGTGGCATTTGCACAAGGTGGACACAAGCCTTGTGTTCAAGCATTTGGTTGTGATCAATTTGATGGAGAAGATCCTCAAGAATGCATAGCCAAATGCTCTTTCTTCAACTGGTGGTACTTTGCCACTACTTTTGGATCCTTCATTGCTCTAATCATTTTAAACTACATTCAAGATAACCTCGGTTGGGGTCTTGGCTTCGGCATTCCCTGCATCTCCTCCGTAGCTGCTCTCGTTGTGTTCTTGCTCGGGACTCGGACTTATCGTTTCGTTACAGTGGCCAACAATGAGGAGAAAACATTCATGAGAATTGGTCGGGTTTTTGTCAATGCGGCTCGAAACTGGAGAACGACATCGTCGGACATTGCTATTTTGGAGGAAGGTCAAGATGCAATGCTTTATCAAAGATATGGACAATTGAGGTTCCTTAACAAAGCCTTGGTTGCTCGAATGGACTCGAATAAAGATTTGAAAACGTGTAACATAGTGGAGGTTGAAGAAGCTAAGGGTATCCTAAGGCTGATCCCCATTTGGACAGCAAGCTTGGCATATGCCATTGTTTTATCACAATGCTCTACCTTCTTCGTCAAGCAAGCTTCCACCATGGACAGATCAATCTCACCAACCTTCAAAATACCCGCTGCTACAATCCAATGCTTTGCCCTCATTGCTGTTCTCCTCTTCCTTCCAATCTATGACCAACTCCTCGTTCCCATCGCCAGAATATTCACCCTCAAACCCTCCGGAATCTCCATGCTCCAAAGAATTGGAGTTGGGATGTTCATATCCACTCTTTCAATGGTTGTTGCCGCTCTTGTTGAATGTAAAAGGCTAGCCGTCGCTCGAGAACATGGCTTAACTCATAACCCTAACGCTACGATCCCTATCACCATCTGGTGGCTAACTCCACAGCTCATCCTCCTCGGAGTTTCTAGCGTTTTCACGATGGTAGGTTTGCAGGAGTTCTTCTACGACCAAGTTTTGATCGAGCTGAAGAGTGTGGGACTTGCACTGTATCTGAGCATCTTTGGAGTGGGGAACTTGCTTAGCGGTGTTCTTGTATCCATCATTGAAGACACAACTGGTCGACATGGAAGAGGTGGCTGGCTTGCTAACAACATAAATAATGCACATCTTGATTACTTTTATTGGCTTCTTGCTGGCATTGGTGAGGTAGGATTACTTGCATATATGTATTTTGCTAACTCTTATGTATATAAATACAACGTCGAAAGATCTAGAGATTAA

Coding sequence (CDS)

ATGGATATGGCTGATGAATCCCGAAGGCCGCTGATATTAAACGACGACGTCGTACCGGGGTCCGTGGACCACCGAGAGCGTCCGAGCCGGCGATCAGAGTCTGGGCGGTGGAGGGCGGCGGGGTTGATAATTGGAGTTGAAATAGCGGAGCGGTTGGCATTCTTCGGGATAAGCACCAATCTGGTAAGCTACTTGACAGTGGAGATGGGGCAGTCCATGGCCGCCGCCGCCAAGAATGTAAATCTCTGGATCGGAACCGCCTCTTTGCTCCCATTGCTTGCCGCCTCTTTCGCCGATTCCTTCCTCGGACGCTACCTTACTATTCTTCTCGCCTCCGCTCTTTACATCTTGGTTAACCTTCTTTTTCCTTTTCTTTTTCTCATTCTCATTCTTTTAAACATCATCATAATAAACAAATTATCTACGCTCCGGGGATCTGGCTCTGCTGCCACGGGAGCTTCATCTCAGCCTGTTCCTGATGCTGATGTCGTGTTGTTCTTCAGCGCTTTGTACTTGGTGGCATTTGCACAAGGTGGACACAAGCCTTGTGTTCAAGCATTTGGTTGTGATCAATTTGATGGAGAAGATCCTCAAGAATGCATAGCCAAATGCTCTTTCTTCAACTGGTGGTACTTTGCCACTACTTTTGGATCCTTCATTGCTCTAATCATTTTAAACTACATTCAAGATAACCTCGGTTGGGGTCTTGGCTTCGGCATTCCCTGCATCTCCTCCGTAGCTGCTCTCGTTGTGTTCTTGCTCGGGACTCGGACTTATCGTTTCGTTACAGTGGCCAACAATGAGGAGAAAACATTCATGAGAATTGGTCGGGTTTTTGTCAATGCGGCTCGAAACTGGAGAACGACATCGTCGGACATTGCTATTTTGGAGGAAGGTCAAGATGCAATGCTTTATCAAAGATATGGACAATTGAGGTTCCTTAACAAAGCCTTGGTTGCTCGAATGGACTCGAATAAAGATTTGAAAACGTGTAACATAGTGGAGGTTGAAGAAGCTAAGGGTATCCTAAGGCTGATCCCCATTTGGACAGCAAGCTTGGCATATGCCATTGTTTTATCACAATGCTCTACCTTCTTCGTCAAGCAAGCTTCCACCATGGACAGATCAATCTCACCAACCTTCAAAATACCCGCTGCTACAATCCAATGCTTTGCCCTCATTGCTGTTCTCCTCTTCCTTCCAATCTATGACCAACTCCTCGTTCCCATCGCCAGAATATTCACCCTCAAACCCTCCGGAATCTCCATGCTCCAAAGAATTGGAGTTGGGATGTTCATATCCACTCTTTCAATGGTTGTTGCCGCTCTTGTTGAATGTAAAAGGCTAGCCGTCGCTCGAGAACATGGCTTAACTCATAACCCTAACGCTACGATCCCTATCACCATCTGGTGGCTAACTCCACAGCTCATCCTCCTCGGAGTTTCTAGCGTTTTCACGATGGTAGGTTTGCAGGAGTTCTTCTACGACCAAGTTTTGATCGAGCTGAAGAGTGTGGGACTTGCACTGTATCTGAGCATCTTTGGAGTGGGGAACTTGCTTAGCGGTGTTCTTGTATCCATCATTGAAGACACAACTGGTCGACATGGAAGAGGTGGCTGGCTTGCTAACAACATAAATAATGCACATCTTGATTACTTTTATTGGCTTCTTGCTGGCATTGGTGAGGTAGGATTACTTGCATATATGTATTTTGCTAACTCTTATGTATATAAATACAACGTCGAAAGATCTAGAGATTAA

Protein sequence

MDMADESRRPLILNDDVVPGSVDHRERPSRRSESGRWRAAGLIIGVEIAERLAFFGISTNLVSYLTVEMGQSMAAAAKNVNLWIGTASLLPLLAASFADSFLGRYLTILLASALYILVNLLFPFLFLILILLNIIIINKLSTLRGSGSAATGASSQPVPDADVVLFFSALYLVAFAQGGHKPCVQAFGCDQFDGEDPQECIAKCSFFNWWYFATTFGSFIALIILNYIQDNLGWGLGFGIPCISSVAALVVFLLGTRTYRFVTVANNEEKTFMRIGRVFVNAARNWRTTSSDIAILEEGQDAMLYQRYGQLRFLNKALVARMDSNKDLKTCNIVEVEEAKGILRLIPIWTASLAYAIVLSQCSTFFVKQASTMDRSISPTFKIPAATIQCFALIAVLLFLPIYDQLLVPIARIFTLKPSGISMLQRIGVGMFISTLSMVVAALVECKRLAVAREHGLTHNPNATIPITIWWLTPQLILLGVSSVFTMVGLQEFFYDQVLIELKSVGLALYLSIFGVGNLLSGVLVSIIEDTTGRHGRGGWLANNINNAHLDYFYWLLAGIGEVGLLAYMYFANSYVYKYNVERSRD
BLAST of Lsi08G000170 vs. Swiss-Prot
Match: PTR9_ARATH (Protein NRT1/ PTR FAMILY 5.10 OS=Arabidopsis thaliana GN=NPF5.10 PE=2 SV=1)

HSP 1 Score: 605.9 bits (1561), Expect = 4.6e-172
Identity = 320/574 (55.75%), Postives = 405/574 (70.56%), Query Frame = 1

Query: 5   DESRRPLILNDDVVPGSVDHRERPSRRSESGRWRAAGLIIGVEIAERLAFFGISTNLVSY 64
           DE+  PL+        +VD+R +P+ +S SG WR+AG IIGVE+AER A++GIS+NL++Y
Sbjct: 8   DEAGTPLLAV------TVDYRNKPAVKSSSGGWRSAGFIIGVEVAERFAYYGISSNLITY 67

Query: 65  LTVEMGQSMAAAAKNVNLWIGTASLLPLLAASFADSFLGRYLTILLASALYILVNLLFPF 124
           LT  +GQS AAAA NVN W GTASLLPLL A  ADSFLGR+ TIL ASALYI        
Sbjct: 68  LTGPLGQSTAAAAANVNAWSGTASLLPLLGAFVADSFLGRFRTILAASALYI-------- 127

Query: 125 LFLILILLNIIIINKLSTLRGSGSAATGASSQPVPDADVVLFFSALYLVAFAQGGHKPCV 184
                + L ++    LS +  S    +   S   P   V+ FFSALYLVA AQGGHKPCV
Sbjct: 128 -----VGLGVL---TLSAMIPSDCKVSNLLSSCSPRFQVITFFSALYLVALAQGGHKPCV 187

Query: 185 QAFGCDQFDGEDPQECIAKCSFFNWWYFATTFGSFIALIILNYIQDNLGWGLGFGIPCIS 244
           QAFG DQFD ++P+EC AK SFFNWWYF   FG+   L +LNYIQDNL W LGFGIPCI+
Sbjct: 188 QAFGADQFDEKEPEECKAKSSFFNWWYFGMCFGTLTTLWVLNYIQDNLSWALGFGIPCIA 247

Query: 245 SVAALVVFLLGTRTYRFVTVANNEEKTFMRIGRVFVNAARNWRTTSSDIAILEEGQDAML 304
            V ALVV LLGT TYRF ++   ++  F+RIG V+V A +NW  ++ D+A  EE    + 
Sbjct: 248 MVVALVVLLLGTCTYRF-SIRREDQSPFVRIGNVYVAAVKNWSVSALDVAAAEERLGLVS 307

Query: 305 YQRYGQLRFLNKALVARMDSNKDLKTCNIVEVEEAKGILRLIPIWTASLAYAIVLSQCST 364
                Q  FLNKALVA+  S      C+I E+EEAK +LRL PIW   L YA+V +Q  T
Sbjct: 308 CSSSQQFSFLNKALVAKNGS------CSIDELEEAKSVLRLAPIWLTCLVYAVVFAQSPT 367

Query: 365 FFVKQASTMDRSISPTFKIPAATIQCFALIAVLLFLPIYDQLLVPIARIFTLKPSGISML 424
           FF KQ +TM+RSI+P +KI  AT+Q F  +++++F+PIYD++L+PIAR FT KP GI+ML
Sbjct: 368 FFTKQGATMERSITPGYKISPATLQSFISLSIVIFIPIYDRVLIPIARSFTHKPGGITML 427

Query: 425 QRIGVGMFISTLSMVVAALVECKRLAVAREHGLTHNPNATIPITIWWLTPQLILLGVSSV 484
           QRIG G+F+S L+MVVAALVE KRL  A ++GL  +P+AT+P+++WWL PQ +L G++ V
Sbjct: 428 QRIGTGIFLSFLAMVVAALVEMKRLKTAADYGLVDSPDATVPMSVWWLVPQYVLFGITDV 487

Query: 485 FTMVGLQEFFYDQVLIELKSVGLALYLSIFGVGNLLSGVLVSIIEDTTGRHGRGGWLANN 544
           F MVGLQEFFYDQV  EL+SVGLALYLSIFG+GN LS  ++SIIE  T + G+  W ANN
Sbjct: 488 FAMVGLQEFFYDQVPNELRSVGLALYLSIFGIGNFLSSFMISIIEKATSQSGQASWFANN 547

Query: 545 INNAHLDYFYWLLAGIGEVGLLAYMYFANSYVYK 579
           +N AHLDYFYWLLA +  +GL +Y+Y A SYV K
Sbjct: 548 LNQAHLDYFYWLLACLSFIGLASYLYVAKSYVSK 552

BLAST of Lsi08G000170 vs. Swiss-Prot
Match: PTR25_ARATH (Protein NRT1/ PTR FAMILY 5.12 OS=Arabidopsis thaliana GN=NPF5.12 PE=2 SV=1)

HSP 1 Score: 531.9 bits (1369), Expect = 8.5e-150
Identity = 288/571 (50.44%), Postives = 380/571 (66.55%), Query Frame = 1

Query: 12  ILNDDVVPGSVDHRERPSRRSESGRWRAAGLIIGVEIAERLAFFGISTNLVSYLTVEMGQ 71
           I++++ V  SVD R  PS RS SG W+++G  +  E+AE+ A+FGI++NL++Y T  +G+
Sbjct: 13  IVSNENVEFSVDFRGNPSIRSSSGAWKSSGFTMCAEVAEKFAYFGIASNLITYFTEALGE 72

Query: 72  SMAAAAKNVNLWIGTASLLPLLAASFADSFLGRYLTILLASALYILVNLLFPFLFLILIL 131
           S A AA NVNLW+GTA+ LPL+  S ADSFLGR+ TILL S+ YI+   L  F   I  L
Sbjct: 73  STAVAASNVNLWLGTAAFLPLIWGSIADSFLGRFRTILLTSSFYIMGLGLLTFSATIPSL 132

Query: 132 LNIIIINKLSTLRGSGSAATGASSQPVPDADVVLFFSALYLVAFAQGGHKPCVQAFGCDQ 191
            N                 T  S   V    V++FF ALYL+A  +GG K C++AFG DQ
Sbjct: 133 CN--------------DQETRESC--VSQVKVIIFFCALYLIALGEGGFKVCLRAFGADQ 192

Query: 192 FDGEDPQECIAKCSFFNWWYFATTFGSFIALIILNYIQDNLGWGLGFGIPCISSVAALVV 251
           FD +DP E  AK S+FNW YFA + G     ++ NY+Q+NL W LG+ IPC+S + AL +
Sbjct: 193 FDEQDPNESKAKSSYFNWLYFAISIGILTTRLVTNYVQENLSWALGYAIPCLSMMLALFL 252

Query: 252 FLLGTRTYRFVT-----VANNEEKTFMRIGRVFVNAARNWRTTSSDIAILEEGQDAMLYQ 311
           FLLG +TYRF T          +  F+RIGRVFV AARN R T SD  +L   +    + 
Sbjct: 253 FLLGIKTYRFSTGGEGRQGKKHDNPFVRIGRVFVAAARNRRQTPSDTCLLLPNESTKKF- 312

Query: 312 RYGQLRFLNKALVARMDSNKDLKTCNIVEVEEAKGILRLIPIWTASLAYAIVLSQCSTFF 371
                RFL++A+++          C+  EVEEAK +L LIPIW  SL + IV +Q  TFF
Sbjct: 313 -----RFLDRAVIS----------CDSYEVEEAKAVLSLIPIWLCSLVFGIVFAQSPTFF 372

Query: 372 VKQASTMDRSISPTFKIPAATIQCFALIAVLLFLPIYDQLLVPIARIFTLKPSGISMLQR 431
            KQ STMDRSIS T ++PAAT+QCF  +A+L+F+PIYD+L VPIAR  T KP+GI+ LQR
Sbjct: 373 TKQGSTMDRSISSTLQVPAATLQCFISLAILVFIPIYDRLFVPIARSITRKPAGITTLQR 432

Query: 432 IGVGMFISTLSMVVAALVECKRLAVAREHGLTHNPNATIPITIWWLTPQLILLGVSSVFT 491
           I  G+F+S +SMV+AALVE KRL  AR+HGL  +P AT+P+++ WL PQ IL GVS VFT
Sbjct: 433 ISTGIFLSIISMVIAALVEMKRLKTARDHGLVDSPKATVPMSVCWLIPQYILFGVSDVFT 492

Query: 492 MVGLQEFFYDQVLIELKSVGLALYLSIFGVGNLLSGVLVSIIEDTTGRHGRGGWLANNIN 551
           MVGLQEFFY +V  +L+S+GLALYLSI G+GN LS  +VS+IE+ T + G+  W +NN+N
Sbjct: 493 MVGLQEFFYGEVPPQLRSMGLALYLSIIGIGNFLSSFMVSVIEEATSQSGQVSWFSNNLN 551

Query: 552 NAHLDYFYWLLAGIGEVGLLAYMYFANSYVY 578
            AHLDYFYWLLA +  +  +  +YFA SY+Y
Sbjct: 553 QAHLDYFYWLLACLSSLAFIFTVYFAKSYLY 551

BLAST of Lsi08G000170 vs. Swiss-Prot
Match: PTR23_ARATH (Protein NRT1/ PTR FAMILY 5.13 OS=Arabidopsis thaliana GN=NPF5.13 PE=2 SV=2)

HSP 1 Score: 514.6 bits (1324), Expect = 1.4e-144
Identity = 277/567 (48.85%), Postives = 379/567 (66.84%), Query Frame = 1

Query: 13  LNDDVVPGSVDHRERPSRRSESGRWRAAGLIIGVEIAERLAFFGISTNLVSYLTVEMGQS 72
           L ++ V  +VDHR   +RRS +GRWRAA  IIGVE+AER A +GI +NL+SYLT  +GQS
Sbjct: 9   LQEEYVIDAVDHRGFSARRSITGRWRAAWFIIGVEVAERFANYGIGSNLISYLTGPLGQS 68

Query: 73  MAAAAKNVNLWIGTASLLPLLAASFADSFLGRYLTILLASALYILVNLLFPFLFLILILL 132
            A AA NVN W G +++LPLL A  AD+FLGRY+TI++AS +Y+L         L  + L
Sbjct: 69  TAVAAANVNAWSGISTILPLLGAFVADAFLGRYITIIIASFIYVLG--------LAFLTL 128

Query: 133 NIIIINKLSTLRGSGSAATGASSQPVPDADVVLFFSALYLVAFAQGGHKPCVQAFGCDQF 192
           +  +I   + +  S S+   A           LFF +LYLVA  Q GHKPCVQAFG DQF
Sbjct: 129 SAFLIPNNTEVTSSPSSFLNA-----------LFFFSLYLVAIGQSGHKPCVQAFGADQF 188

Query: 193 DGEDPQECIAKCSFFNWWYFATTFGSFIALIILNYIQDNLGWGLGFGIPCISSVAALVVF 252
           D ++PQE   + SFFNWWY +   G  +A++++ YIQ+N+ W LGFGIPC+  V +LV+F
Sbjct: 189 DEKNPQENSDRSSFFNWWYLSMCAGIGLAILVVVYIQENVSWALGFGIPCVFMVISLVLF 248

Query: 253 LLGTRTYRFVTVANNEEKT-FMRIGRVFVNAARNWRTTSSDIAILEEGQDAMLYQRYGQL 312
           +LG ++YRF      EE   F RIGRVF  A +N R  SSD+  +E  +     +   +L
Sbjct: 249 VLGRKSYRFSKTRQEEETNPFTRIGRVFFVAFKNQRLNSSDLCKVELIEANRSQESPEEL 308

Query: 313 RFLNKALVARMDSNKDLKTCNIVEVEEAKGILRLIPIWTASLAYAIVLSQCSTFFVKQAS 372
            FLNKAL+   DS++    C   +VE+A  ++RLIP+W  +LAYAI  +Q  TFF KQ  
Sbjct: 309 SFLNKALLVPNDSDEGEVACKSRDVEDATALVRLIPVWLTTLAYAIPFAQYMTFFTKQGV 368

Query: 373 TMDRSISPTFKIPAATIQCFALIAVLLFLPIYDQLLVPIARIFTLKPSGISMLQRIGVGM 432
           TM+R+I P  +IP A++Q    I+++LF+PIYD++LVPI R  T  P GI+ L+RIG GM
Sbjct: 369 TMERTIFPGVEIPPASLQVLISISIVLFVPIYDRVLVPIGRSITKDPCGITTLKRIGTGM 428

Query: 433 FISTLSMVVAALVECKRLAVAREHGLTHNPNATIPITIWWLTPQLILLGVSSVFTMVGLQ 492
            ++TL+MVVAALVE KRL  A+E+GL   P  T+P++IWWL PQ +LLG++ V T+VG+Q
Sbjct: 429 VLATLTMVVAALVESKRLETAKEYGLIDQPKTTLPMSIWWLFPQYMLLGLADVHTLVGMQ 488

Query: 493 EFFYDQVLIELKSVGLALYLSIFGVGNLLSGVLVSIIEDTTGRHGRGGWLANNINNAHLD 552
           EFFY QV  EL+S+GLA+YLS  GVG+LLS +L+ +I+  TG      W  +N+N AHLD
Sbjct: 489 EFFYSQVPTELRSLGLAIYLSAMGVGSLLSSLLIYLIDLATGGDAGNSWFNSNLNRAHLD 548

Query: 553 YFYWLLAGIGEVGLLAYMYFANSYVYK 579
           YFYWLLA +  VG   +++ + SY+Y+
Sbjct: 549 YFYWLLAVVSAVGFFTFLFISKSYIYR 556

BLAST of Lsi08G000170 vs. Swiss-Prot
Match: PTR22_ARATH (Protein NRT1/ PTR FAMILY 5.14 OS=Arabidopsis thaliana GN=NPF5.14 PE=2 SV=2)

HSP 1 Score: 513.5 bits (1321), Expect = 3.1e-144
Identity = 275/567 (48.50%), Postives = 378/567 (66.67%), Query Frame = 1

Query: 13  LNDDVVPGSVDHRERPSRRSESGRWRAAGLIIGVEIAERLAFFGISTNLVSYLTVEMGQS 72
           L ++ V  +VDHR   +RRS +GRWRAA  IIGVE+AER A++GI +NL+SYLT  +G+S
Sbjct: 9   LQEEYVTDAVDHRGLAARRSNTGRWRAALFIIGVEVAERFAYYGIGSNLISYLTGPLGES 68

Query: 73  MAAAAKNVNLWIGTASLLPLLAASFADSFLGRYLTILLASALYILVNLLFPFLFLILILL 132
            A AA NVN W G A+LLP+L A  AD+FLGRY TI+++S +Y+L         L  + L
Sbjct: 69  TAVAAANVNAWSGIATLLPVLGAFVADAFLGRYRTIIISSLIYVLG--------LAFLTL 128

Query: 133 NIIIINKLSTLRGSGSAATGASSQPVPDADVVLFFSALYLVAFAQGGHKPCVQAFGCDQF 192
           +  +I   + +  S S+              VLFF +LYLVA  Q GHKPCVQAFG DQF
Sbjct: 129 SAFLIPNTTEVTSSTSSFLN-----------VLFFFSLYLVAIGQSGHKPCVQAFGADQF 188

Query: 193 DGEDPQECIAKCSFFNWWYFATTFGSFIALIILNYIQDNLGWGLGFGIPCISSVAALVVF 252
           D +D QE   + SFFNWWY + + G   A++++ YIQ+   W  GFGIPC+  V +LV+F
Sbjct: 189 DEKDSQEKSDRSSFFNWWYLSLSAGICFAILVVVYIQEEFSWAFGFGIPCVFMVISLVLF 248

Query: 253 LLGTRTYRFVTVANNEE-KTFMRIGRVFVNAARNWRTTSSDIAILEEGQDAMLYQRYGQL 312
           + G R YR+    + EE   F RIGRVF  A +N R +SSD+  +E   +    ++    
Sbjct: 249 VSGRRIYRYSKRRHEEEINPFTRIGRVFFVALKNQRLSSSDLCKVELEANTSPEKQ---- 308

Query: 313 RFLNKALVARMDSNKDLKTCNIVEVEEAKGILRLIPIWTASLAYAIVLSQCSTFFVKQAS 372
            F NKAL+   DS++        +VE+A  ++RLIP+W  +LAYAI  +Q  TFF KQ  
Sbjct: 309 SFFNKALLVPNDSSQGENASKSSDVEDATALIRLIPVWFTTLAYAIPYAQYMTFFTKQGV 368

Query: 373 TMDRSISPTFKIPAATIQCFALIAVLLFLPIYDQLLVPIARIFTLKPSGISMLQRIGVGM 432
           TMDR+I P  KIP A++Q F  I+++LF+PIYD++ VPIAR+ T +P GI+ L+RIG G+
Sbjct: 369 TMDRTILPGVKIPPASLQVFIGISIVLFVPIYDRVFVPIARLITKEPCGITTLKRIGTGI 428

Query: 433 FISTLSMVVAALVECKRLAVAREHGLTHNPNATIPITIWWLTPQLILLGVSSVFTMVGLQ 492
            +ST++MV+AALVE KRL  A+EHGL   P AT+P++IWWL PQ +LLG++ V+T+VG+Q
Sbjct: 429 VLSTITMVIAALVEFKRLETAKEHGLIDQPEATLPMSIWWLIPQYLLLGLADVYTLVGMQ 488

Query: 493 EFFYDQVLIELKSVGLALYLSIFGVGNLLSGVLVSIIEDTTGRHGRGGWLANNINNAHLD 552
           EFFY QV  EL+S+GLALYLS  GVG+LLS +L+S+I+  TG      W  +N+N AHLD
Sbjct: 489 EFFYSQVPTELRSIGLALYLSALGVGSLLSSLLISLIDLATGGDAGNSWFNSNLNRAHLD 548

Query: 553 YFYWLLAGIGEVGLLAYMYFANSYVYK 579
           YFYWLLA +  VG   +++ + SY+Y+
Sbjct: 549 YFYWLLAIVSAVGFFTFLFISKSYIYR 552

BLAST of Lsi08G000170 vs. Swiss-Prot
Match: PTR10_ARATH (Protein NRT1/ PTR FAMILY 5.16 OS=Arabidopsis thaliana GN=NPF5.16 PE=2 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 7.2e-141
Identity = 276/568 (48.59%), Postives = 380/568 (66.90%), Query Frame = 1

Query: 13  LNDDVVPGSVDHRERPSRRSESGRWRAAGLIIGVEIAERLAFFGISTNLVSYLTVEMGQS 72
           L +D V  SVDHR  P+ +S +G WR+A  IIGVE+ ER A+FGI +NL++YLT  +GQS
Sbjct: 10  LIEDSVSDSVDHRGLPAGKSSTGGWRSAWYIIGVEVGERFAYFGIGSNLITYLTGPLGQS 69

Query: 73  MAAAAKNVNLWIGTASLLPLLAASFADSFLGRYLTILLASALYILVNLLFPFLFLILILL 132
            A AA NVN W GTAS+LP+L A  AD++LGRY TI++AS +YIL          +L L 
Sbjct: 70  TATAAVNVNTWSGTASILPVLGAFIADAYLGRYRTIVVASLIYILG-------LGLLTLS 129

Query: 133 NIIIINKLSTLRGSGSAATGASSQPVPDADVVLFFSALYLVAFAQGGHKPCVQAFGCDQF 192
           +I+I+  LS  R        AS++P    ++ LFF +LYLVA  QGGHKPCVQAFG DQF
Sbjct: 130 SILILMGLSEQRQHNR---NASAKPFFWVNI-LFFCSLYLVAIGQGGHKPCVQAFGADQF 189

Query: 193 DGEDPQECIAKCSFFNWWYFATTFGSFIALIILNYIQDNLGWGLGFGIPCISSVAALVVF 252
           D  DP+E I++ SFFNWW+ + + G  +++I++ Y+QDN+ W LGFGIPC+  V AL +F
Sbjct: 190 DVGDPKERISRGSFFNWWFLSLSAGITLSIIVVVYVQDNVNWALGFGIPCLFMVMALALF 249

Query: 253 LLGTRTYRFVTV-ANNEEKTFMRIGRVFVNAARNWRTTSSDIAILEEGQDAMLYQRY-GQ 312
           L G +TYR+       +   F RIGRVF+ A +N +   +    LE G     Y++  GQ
Sbjct: 250 LFGRKTYRYPRGDREGKNNAFARIGRVFLVAFKNRKLKLTHSGQLEVGS----YKKCKGQ 309

Query: 313 LRFLNKALVARMDSNKDLKTCNIVEVEEAKGILRLIPIWTASLAYAIVLSQCSTFFVKQA 372
           L FL KAL   +     ++ C+  +VE+A  ++RLIPIW  S+   I  +Q +TFF KQ 
Sbjct: 310 LEFLAKAL---LPGEGGVEPCSSRDVEDAMALVRLIPIWITSVVSTIPYAQYATFFTKQG 369

Query: 373 STMDRSISPTFKIPAATIQCFALIAVLLFLPIYDQLLVPIARIFTLKPSGISMLQRIGVG 432
            T+DR I P F+IP A+ Q    +++ + +P Y+++ +P+AR+ T KPSGI+MLQRIG G
Sbjct: 370 VTVDRKILPGFEIPPASFQALIGLSIFISVPTYERVFLPLARLITKKPSGITMLQRIGAG 429

Query: 433 MFISTLSMVVAALVECKRLAVAREHGLTHNPNATIPITIWWLTPQLILLGVSSVFTMVGL 492
           M +S+L+MVVAALVE KRL  A+EHGL   P+ATIP++IWW  PQ +LLG+  VF++VG 
Sbjct: 430 MVLSSLNMVVAALVEMKRLETAKEHGLVDRPDATIPMSIWWFVPQYLLLGMIDVFSLVGT 489

Query: 493 QEFFYDQVLIELKSVGLALYLSIFGVGNLLSGVLVSIIEDTTGRHGRGGWLANNINNAHL 552
           QEFFYDQV  EL+S+GLAL LS  G+ + LSG L+++I   TG++G   W   N+N AH+
Sbjct: 490 QEFFYDQVPTELRSIGLALSLSAMGLASFLSGFLITVINWATGKNGGDSWFNTNLNRAHV 549

Query: 553 DYFYWLLAGIGEVGLLAYMYFANSYVYK 579
           DYFYWLLA    +G LA++  +  YVY+
Sbjct: 550 DYFYWLLAAFTAIGFLAFLLLSRLYVYR 559

BLAST of Lsi08G000170 vs. TrEMBL
Match: U5FXL3_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0013s11120g PE=3 SV=1)

HSP 1 Score: 646.7 bits (1667), Expect = 2.6e-182
Identity = 340/580 (58.62%), Postives = 426/580 (73.45%), Query Frame = 1

Query: 3   MADESRRPLILNDDVVPGSVDHRERPSRRSESGRWRAAGLIIGVEIAERLAFFGISTNLV 62
           M+  ++ PL L+ D+V GSVD++ RP+ RS SG WR+A  IIGVE+AER A++GIS+NL+
Sbjct: 1   MSSSNQTPL-LSYDIVDGSVDYKSRPAYRSNSGGWRSASFIIGVEVAERFAYYGISSNLI 60

Query: 63  SYLTVEMGQSMAAAAKNVNLWIGTASLLPLLAASFADSFLGRYLTILLASALYIL-VNLL 122
           +YLT  +GQS A AA+NVN+W GTASLLPLL A  ADSFLGRY TI+ AS +YIL + LL
Sbjct: 61  TYLTGPLGQSTATAAENVNVWAGTASLLPLLGAFVADSFLGRYRTIIFASLIYILGLGLL 120

Query: 123 FPFLFLILILLNIIIINKLSTLRGSGSAATGASSQPVPDADV----VLFFSALYLVAFAQ 182
                             LS L  S +AATG  S  +P + V    +LFF ALYLVA  Q
Sbjct: 121 -----------------TLSALLPSHAAATGFRSTNLPSSPVPFQLILFFFALYLVAIGQ 180

Query: 183 GGHKPCVQAFGCDQFDGEDPQECIAKCSFFNWWYFATTFGSFIALIILNYIQDNLGWGLG 242
            GHKPCVQAFG DQFDG+DPQE  AK SFFNWWY     G+ + L++LNYIQDNL WGLG
Sbjct: 181 SGHKPCVQAFGADQFDGQDPQESKAKSSFFNWWYCFMCAGTVVTLLVLNYIQDNLNWGLG 240

Query: 243 FGIPCISSVAALVVFLLGTRTYRFVTVANNEEKTFMRIGRVFVNAARNWRTTSSDIAILE 302
           FGIPC   V AL++FLLGT+TYR+ ++  +E+  F+RIG VFV++ RNWRTT S IA  E
Sbjct: 241 FGIPCAVMVIALIIFLLGTKTYRY-SIKGDEKSAFLRIGWVFVSSIRNWRTTPSAIAFEE 300

Query: 303 EGQDAMLYQRYGQLRFLNKALVARMDSNKDLKTCNIVEVEEAKGILRLIPIWTASLAYAI 362
           E +  + +Q   Q +FLNKAL+A     +D K C++ +VEEAK +LRL+PIW   L +AI
Sbjct: 301 ETRGTLPHQSSEQYKFLNKALLAPNGLKEDGKVCSLGDVEEAKALLRLVPIWATCLVFAI 360

Query: 363 VLSQCSTFFVKQASTMDRSISPTFKIPAATIQCFALIAVLLFLPIYDQLLVPIARIFTLK 422
           V +Q STFF KQ  TMDRSISP+  +PAA++Q F  ++++LF+PIYD++LVPIAR  T K
Sbjct: 361 VFAQTSTFFTKQGVTMDRSISPSLDLPAASLQSFISLSIVLFIPIYDRVLVPIARALTRK 420

Query: 423 PSGISMLQRIGVGMFISTLSMVVAALVECKRLAVAREHGLTHNPNATIPITIWWLTPQLI 482
           PSGI+MLQRIG GMFIS ++M+VAALVE KRL  A+EHGL   PN TIP+++WWL PQ +
Sbjct: 421 PSGITMLQRIGSGMFISVVAMIVAALVEMKRLKTAQEHGLVDLPNVTIPMSVWWLIPQYV 480

Query: 483 LLGVSSVFTMVGLQEFFYDQVLIELKSVGLALYLSIFGVGNLLSGVLVSIIEDTTGRHGR 542
           L GV+  FTMVGLQEFFYDQV  +L+SVGL+LYLSIFGVG+ LS  L+SIIE  TG +GR
Sbjct: 481 LFGVAEAFTMVGLQEFFYDQVPSDLRSVGLSLYLSIFGVGSFLSSFLISIIEKATGGNGR 540

Query: 543 GGWLANNINNAHLDYFYWLLAGIGEVGLLAYMYFANSYVY 578
             W ANN+N AHLDYFYWLLAG+  V L+AY+ FA SY+Y
Sbjct: 541 YSWFANNLNRAHLDYFYWLLAGLSAVQLVAYVCFAKSYIY 561

BLAST of Lsi08G000170 vs. TrEMBL
Match: A0A059BVA5_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_F02994 PE=3 SV=1)

HSP 1 Score: 637.5 bits (1643), Expect = 1.6e-179
Identity = 332/574 (57.84%), Postives = 419/574 (73.00%), Query Frame = 1

Query: 4   ADESRRPLILNDDVVPGSVDHRERPSRRSESGRWRAAGLIIGVEIAERLAFFGISTNLVS 63
           ++++  PL+   D V G+VD+  RP  RS SG WR+AG IIGVE+AER A++GI++NL++
Sbjct: 12  SNDAETPLL--QDAVDGAVDYGGRPVARSASGGWRSAGFIIGVEVAERFAYYGIASNLIT 71

Query: 64  YLTVEMGQSMAAAAKNVNLWIGTASLLPLLAASFADSFLGRYLTILLASALYILVNLLFP 123
           YLT  +GQS AAAA NVN W GTASL PL+ A  ADSFLGRY TI+++S LYIL      
Sbjct: 72  YLTGPLGQSTAAAAANVNAWSGTASLSPLIGAFIADSFLGRYRTIVVSSLLYILG----- 131

Query: 124 FLFLILILLNIIIINKLSTLRGSGSAATGASSQPVPDADVVLFFSALYLVAFAQGGHKPC 183
              L L+ L+ ++ +  ++   S SA       P P   + LFF +LYLVAFAQGGHKPC
Sbjct: 132 ---LGLLTLSAMLPSLGTSDCQSNSAIQNLPCFP-PLYQLALFFFSLYLVAFAQGGHKPC 191

Query: 184 VQAFGCDQFDGEDPQECIAKCSFFNWWYFATTFGSFIALIILNYIQDNLGWGLGFGIPCI 243
           VQAFG DQFDG++P+EC AK SFFNWWYF    G+F  L+I+NYIQDNL WGLGFGIPC+
Sbjct: 192 VQAFGADQFDGQNPEECKAKSSFFNWWYFCMCGGTFATLLIVNYIQDNLNWGLGFGIPCV 251

Query: 244 SSVAALVVFLLGTRTYRFVTVANNEEKTFMRIGRVFVNAARNWRTTSSDIAILEEGQDAM 303
           S  AALV+FLLGT+TYR+ ++ ++E   F RIG VF  AARNW+TT+  +A  EE +  +
Sbjct: 252 SMGAALVLFLLGTKTYRY-SIKSDERNPFSRIGSVFATAARNWKTTTKALAADEESRGTL 311

Query: 304 LYQRYGQLRFLNKALVARMDSNKDLKTCNIVEVEEAKGILRLIPIWTASLAYAIVLSQCS 363
            Y+  GQ +FLNKAL++   S  D K C + EVEEAK +LRL PIW  SL YA+V +Q S
Sbjct: 312 PYEGSGQFKFLNKALLSPDGSKGDSKVCTVGEVEEAKSVLRLTPIWATSLIYAVVFAQSS 371

Query: 364 TFFVKQASTMDRSISPTFKIPAATIQCFALIAVLLFLPIYDQLLVPIARIFTLKPSGISM 423
           TFF KQ  TMDRSI+P F +P A++Q F  ++V++F+PIYD++ VP+AR FT +PSGI+M
Sbjct: 372 TFFTKQGVTMDRSITPGFDVPPASLQSFISLSVVMFIPIYDRVFVPLARSFTQRPSGITM 431

Query: 424 LQRIGVGMFISTLSMVVAALVECKRLAVAREHGLTHNPNATIPITIWWLTPQLILLGVSS 483
           LQRIG+GMF+S +SM VA LVE KRL  AREHGL   PN T+P+++WWL PQ +L G+S 
Sbjct: 432 LQRIGIGMFLSAVSMAVATLVEIKRLETAREHGLVDMPNVTVPMSVWWLIPQYVLFGISD 491

Query: 484 VFTMVGLQEFFYDQVLIELKSVGLALYLSIFGVGNLLSGVLVSIIEDTTGRHGRGGWLAN 543
           VFTMVGLQEFFYDQV  EL+SVGL+LYLSIFGVG+ LS  LVS I   TG  G   W AN
Sbjct: 492 VFTMVGLQEFFYDQVPSELRSVGLSLYLSIFGVGSFLSSFLVSAIATATGGDGGDSWFAN 551

Query: 544 NINNAHLDYFYWLLAGIGEVGLLAYMYFANSYVY 578
           N+N AHLDYFYWLLAG+    L+ Y+YFA SY+Y
Sbjct: 552 NLNKAHLDYFYWLLAGLSAFELVVYLYFARSYIY 573

BLAST of Lsi08G000170 vs. TrEMBL
Match: M5VYB7_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa003472mg PE=3 SV=1)

HSP 1 Score: 635.6 bits (1638), Expect = 6.1e-179
Identity = 334/565 (59.12%), Postives = 412/565 (72.92%), Query Frame = 1

Query: 13  LNDDVVPGSVDHRERPSRRSESGRWRAAGLIIGVEIAERLAFFGISTNLVSYLTVEMGQS 72
           L DDVV G+VD++  P  RS SG WR+A  IIGVE+AER A++GIS NL+++LT  +GQS
Sbjct: 15  LLDDVVEGAVDYKGLPVHRSRSGGWRSAWFIIGVEVAERFAYYGISCNLITFLTGPLGQS 74

Query: 73  MAAAAKNVNLWIGTASLLPLLAASFADSFLGRYLTILLASALYILVNLLFPFLFLILILL 132
            A AA+NVN+W GTASLLPLL A  ADSFLGRY TI+ AS LYIL         L L+ L
Sbjct: 75  TATAAENVNIWSGTASLLPLLGAFVADSFLGRYRTIVFASLLYILG--------LGLLTL 134

Query: 133 NIIIINKLSTLRGSGSAATGASSQPVPDADVVLFFSALYLVAFAQGGHKPCVQAFGCDQF 192
           + ++ +   +     + +T  SSQ      V+ FF +LYLVA AQGGHKPCVQAFG DQF
Sbjct: 135 SAVLPSLTGSYCQKFNQSTSCSSQ----FQVLFFFFSLYLVAVAQGGHKPCVQAFGADQF 194

Query: 193 DGEDPQECIAKCSFFNWWYFATTFGSFIALIILNYIQDNLGWGLGFGIPCISSVAALVVF 252
           D  DP+EC AK SFFNWWYF+  FG+   LI+L YIQDNL WGLGFGIPCI+ V AL +F
Sbjct: 195 DASDPEECKAKSSFFNWWYFSLCFGTTFTLILLTYIQDNLSWGLGFGIPCIAMVLALFIF 254

Query: 253 LLGTRTYRFVTVANNEEKTFMRIGRVFVNAARNWRTTSSDIAILEEGQDAMLYQRYGQLR 312
           LLGTRTYR  ++  +EE  F+RIGRVFV A RNWRTT S I   EE +  + ++   Q +
Sbjct: 255 LLGTRTYR-CSIKGDEESPFVRIGRVFVAALRNWRTTPSAITCEEESRGTLPHKSSEQFK 314

Query: 313 FLNKALVARMDSNKDLKTCNIVEVEEAKGILRLIPIWTASLAYAIVLSQCSTFFVKQAST 372
           FLNKAL+A  D  +  K C + EVEEAK +LRL PIW   LAYA+V +Q STFF KQ +T
Sbjct: 315 FLNKALLAPDDLKESRKVCTVAEVEEAKSVLRLFPIWVTCLAYAVVFAQYSTFFTKQGAT 374

Query: 373 MDRSISPTFKIPAATIQCFALIAVLLFLPIYDQLLVPIARIFTLKPSGISMLQRIGVGMF 432
           MDR+I+P   IPAA++Q F  I +++F+PIYD++ VPIAR+FT KPSGI+MLQRIG+GMF
Sbjct: 375 MDRTIAPGLDIPAASLQTFISITIVIFVPIYDRIFVPIARVFTRKPSGITMLQRIGIGMF 434

Query: 433 ISTLSMVVAALVECKRLAVAREHGLTHNPNATIPITIWWLTPQLILLGVSSVFTMVGLQE 492
           IS +SMVVAALVE KRL  A+++GL   P+AT+P++IWWL PQ +L G++ VFTMVGLQE
Sbjct: 435 ISIISMVVAALVEMKRLKTAKDYGLLDMPSATVPMSIWWLVPQYLLAGLADVFTMVGLQE 494

Query: 493 FFYDQVLIELKSVGLALYLSIFGVGNLLSGVLVSIIEDTTGRHGRGGWLANNINNAHLDY 552
           FFYDQV  ELKS+GLALYLSIFGVG+ LS  L+S I+D T   G   W + N+N AHLDY
Sbjct: 495 FFYDQVPNELKSIGLALYLSIFGVGSFLSSFLISAIDDATTWAGETSWFSTNLNRAHLDY 554

Query: 553 FYWLLAGIGEVGLLAYMYFANSYVY 578
           FYWLL GI  V L  Y+YFA SY+Y
Sbjct: 555 FYWLLGGISVVELAIYLYFAKSYIY 566

BLAST of Lsi08G000170 vs. TrEMBL
Match: A0A022Q2D5_ERYGU (Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a003626mg PE=4 SV=1)

HSP 1 Score: 632.9 bits (1631), Expect = 4.0e-178
Identity = 328/568 (57.75%), Postives = 411/568 (72.36%), Query Frame = 1

Query: 13  LNDDVVPGSVDHRERPSRRSESGRWRAAGLIIGVEIAERLAFFGISTNLVSYLTVEMGQS 72
           L +DVV GSVD ++RP  RS+SG WR+A  IIGVE+AER A++GIS+NL+SYLT  +GQS
Sbjct: 15  LLNDVVEGSVDFKDRPVSRSKSGGWRSASFIIGVEVAERFAYYGISSNLISYLTGPLGQS 74

Query: 73  MAAAAKNVNLWIGTASLLPLLAASFADSFLGRYLTILLASALYILVNLLFPFLFLILILL 132
            A AA+NVNLW GTASLLPLL A  ADSFLGRY  I+ AS LYIL              L
Sbjct: 75  TATAAENVNLWSGTASLLPLLGAFLADSFLGRYRMIIAASVLYILG-------------L 134

Query: 133 NIIIINKLSTLRGSG---SAATGASSQPVPDADVVLFFSALYLVAFAQGGHKPCVQAFGC 192
             + ++ L    GS    +AA  AS  P P   +++FF +LY+VA  QGGHKPCVQAFG 
Sbjct: 135 GCLTLSALVYSLGSSDCENAANNASCSP-PRLQIIVFFFSLYIVALGQGGHKPCVQAFGA 194

Query: 193 DQFDGEDPQECIAKCSFFNWWYFATTFGSFIALIILNYIQDNLGWGLGFGIPCISSVAAL 252
           DQFD +DP+EC AK SFFNWWYF+   G  +AL +LNYIQDNL WGLGFGIPCI    AL
Sbjct: 195 DQFDEQDPEECKAKSSFFNWWYFSFCAGVLVALSVLNYIQDNLSWGLGFGIPCIVMCLAL 254

Query: 253 VVFLLGTRTYRFVTVANNEEKTFMRIGRVFVNAARNWRTTSSDIAILEEGQDAMLYQRYG 312
           +VFLLGT T+RF  V ++E   F+RIG VFV AA+NW T  S I++  E Q  + Y+ + 
Sbjct: 255 IVFLLGTFTFRF-RVNSDEISPFLRIGHVFVRAAKNWHTNPSAISMEAEAQGVLPYEGFE 314

Query: 313 QLRFLNKALVARMDSNKDLKTCNIVEVEEAKGILRLIPIWTASLAYAIVLSQCSTFFVKQ 372
           Q +FLNKAL+    S++D K C+I ++EEAK +LRL+PIW   L YA+V SQ ST F KQ
Sbjct: 315 QYKFLNKALLGSKGSSQDSKVCSITDIEEAKAVLRLVPIWATCLIYAVVFSQSSTLFTKQ 374

Query: 373 ASTMDRSISPTFKIPAATIQCFALIAVLLFLPIYDQLLVPIARIFTLKPSGISMLQRIGV 432
             TMDR I+ TF+IP A++Q    ++++LF+PIYD++LVPIAR  + KPSGISMLQRIG 
Sbjct: 375 GVTMDRHITSTFQIPPASLQSLISLSIVLFIPIYDRILVPIARSISRKPSGISMLQRIGS 434

Query: 433 GMFISTLSMVVAALVECKRLAVAREHGLTHNPNATIPITIWWLTPQLILLGVSSVFTMVG 492
           G+F+S LSMV+AALVE KRLA A EHGL  +P A +P+++WWL PQ +L GVS VFTMVG
Sbjct: 435 GIFLSLLSMVIAALVERKRLATAAEHGLVDDPKAVVPMSVWWLAPQYLLFGVSDVFTMVG 494

Query: 493 LQEFFYDQVLIELKSVGLALYLSIFGVGNLLSGVLVSIIEDTTGRHGRGGWLANNINNAH 552
           LQEFFYDQV  EL+S+GLALYLSIFGVG+ +S  L+S+IE  T    +  W ++N+N AH
Sbjct: 495 LQEFFYDQVPTELRSIGLALYLSIFGVGSFISSFLISVIEKYTSGPSKDSWFSDNLNRAH 554

Query: 553 LDYFYWLLAGIGEVGLLAYMYFANSYVY 578
           +DYFYWLLAG   V  +AY+YFA SY+Y
Sbjct: 555 IDYFYWLLAGFSGVAFIAYVYFAKSYIY 567

BLAST of Lsi08G000170 vs. TrEMBL
Match: M1D3I7_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG401031322 PE=3 SV=1)

HSP 1 Score: 629.8 bits (1623), Expect = 3.3e-177
Identity = 322/562 (57.30%), Postives = 413/562 (73.49%), Query Frame = 1

Query: 16  DVVPGSVDHRERPSRRSESGRWRAAGLIIGVEIAERLAFFGISTNLVSYLTVEMGQSMAA 75
           DVV GSVD++ RP  RS+SG WR+A  IIGVE+AER A++GI +NL+SYLT  +GQS+A+
Sbjct: 17  DVVEGSVDYKGRPVTRSKSGGWRSASFIIGVEVAERFAYYGIGSNLISYLTGPLGQSVAS 76

Query: 76  AAKNVNLWIGTASLLPLLAASFADSFLGRYLTILLASALYILVNLLFPFLFLILILLNII 135
           AA+NVN+W GTASLLPLL A  ADS+LGRY TI+++S LYIL                + 
Sbjct: 77  AAENVNVWSGTASLLPLLGAFIADSYLGRYRTIIISSVLYIL---------------GLG 136

Query: 136 IINKLSTLRGSGSAATGASSQPVPDADVVLFFSALYLVAFAQGGHKPCVQAFGCDQFDGE 195
            +   + L  S S     ++   P   ++ FF +LYLVAF QGGHKPCVQAFG DQFD +
Sbjct: 137 FLTLSTVLPSSHSDCQDTATCSPPKFQIIFFFFSLYLVAFGQGGHKPCVQAFGADQFDAQ 196

Query: 196 DPQECIAKCSFFNWWYFATTFGSFIALIILNYIQDNLGWGLGFGIPCISSVAALVVFLLG 255
           DP+E  AK SFFNWWYF    G F+ L ILNY+QDNL WGLGFGIPCI    ALVVFLLG
Sbjct: 197 DPEESKAKSSFFNWWYFGMCGGLFMTLCILNYVQDNLSWGLGFGIPCIVMGLALVVFLLG 256

Query: 256 TRTYRFVTVANNEEKTFMRIGRVFVNAARNWRTTSSDIAILEEGQDAMLYQRYGQLRFLN 315
           + TYRF   +++E+  F RIG VF+NAARNW+TT+S I++ +E Q  + ++   Q +FLN
Sbjct: 257 SFTYRF-RQSSDEKNPFNRIGNVFINAARNWQTTTSAISVEQEVQGILPHEGSEQFKFLN 316

Query: 316 KALVARMDSNKDLKTCNIVEVEEAKGILRLIPIWTASLAYAIVLSQCSTFFVKQASTMDR 375
           KAL+A   S ++ K C+I EVEEAK ILRLIPIWT  L YAIV SQ ST F KQ +TMDR
Sbjct: 317 KALLAPNGSKENGKICSISEVEEAKAILRLIPIWTTCLVYAIVFSQSSTLFTKQGATMDR 376

Query: 376 SISPTFKIPAATIQCFALIAVLLFLPIYDQLLVPIARIFTLKPSGISMLQRIGVGMFIST 435
           S+   F++PAA++Q F  ++V++F+PIYD++LVP+AR  T KPSGI+MLQRIG G+F+S 
Sbjct: 377 SLGSNFEVPAASLQSFISLSVVIFIPIYDRILVPVARAITGKPSGITMLQRIGTGIFLSI 436

Query: 436 LSMVVAALVECKRLAVAREHGLTHNPNATIPITIWWLTPQLILLGVSSVFTMVGLQEFFY 495
           LSMVVAA++E KRL  A EHGL   P AT+P++I WL PQ IL G+S VFTMVGLQEFFY
Sbjct: 437 LSMVVAAIIEKKRLQTALEHGLVDMPKATVPMSICWLIPQYILFGISDVFTMVGLQEFFY 496

Query: 496 DQVLIELKSVGLALYLSIFGVGNLLSGVLVSIIEDTTGRHGRGGWLANNINNAHLDYFYW 555
           DQV +ELKS+GL+LYLSIFG+G+ LS  L+S+ E+ TG+ G+  W ++N+N AHLDYFYW
Sbjct: 497 DQVPVELKSIGLSLYLSIFGIGSFLSSFLISVTENITGKDGQTSWFSDNLNRAHLDYFYW 556

Query: 556 LLAGIGEVGLLAYMYFANSYVY 578
           +LA +  +   AY+YF+ SY+Y
Sbjct: 557 VLAVLSTIAFTAYLYFSRSYIY 562

BLAST of Lsi08G000170 vs. TAIR10
Match: AT1G22540.1 (AT1G22540.1 Major facilitator superfamily protein)

HSP 1 Score: 605.9 bits (1561), Expect = 2.6e-173
Identity = 320/574 (55.75%), Postives = 405/574 (70.56%), Query Frame = 1

Query: 5   DESRRPLILNDDVVPGSVDHRERPSRRSESGRWRAAGLIIGVEIAERLAFFGISTNLVSY 64
           DE+  PL+        +VD+R +P+ +S SG WR+AG IIGVE+AER A++GIS+NL++Y
Sbjct: 8   DEAGTPLLAV------TVDYRNKPAVKSSSGGWRSAGFIIGVEVAERFAYYGISSNLITY 67

Query: 65  LTVEMGQSMAAAAKNVNLWIGTASLLPLLAASFADSFLGRYLTILLASALYILVNLLFPF 124
           LT  +GQS AAAA NVN W GTASLLPLL A  ADSFLGR+ TIL ASALYI        
Sbjct: 68  LTGPLGQSTAAAAANVNAWSGTASLLPLLGAFVADSFLGRFRTILAASALYI-------- 127

Query: 125 LFLILILLNIIIINKLSTLRGSGSAATGASSQPVPDADVVLFFSALYLVAFAQGGHKPCV 184
                + L ++    LS +  S    +   S   P   V+ FFSALYLVA AQGGHKPCV
Sbjct: 128 -----VGLGVL---TLSAMIPSDCKVSNLLSSCSPRFQVITFFSALYLVALAQGGHKPCV 187

Query: 185 QAFGCDQFDGEDPQECIAKCSFFNWWYFATTFGSFIALIILNYIQDNLGWGLGFGIPCIS 244
           QAFG DQFD ++P+EC AK SFFNWWYF   FG+   L +LNYIQDNL W LGFGIPCI+
Sbjct: 188 QAFGADQFDEKEPEECKAKSSFFNWWYFGMCFGTLTTLWVLNYIQDNLSWALGFGIPCIA 247

Query: 245 SVAALVVFLLGTRTYRFVTVANNEEKTFMRIGRVFVNAARNWRTTSSDIAILEEGQDAML 304
            V ALVV LLGT TYRF ++   ++  F+RIG V+V A +NW  ++ D+A  EE    + 
Sbjct: 248 MVVALVVLLLGTCTYRF-SIRREDQSPFVRIGNVYVAAVKNWSVSALDVAAAEERLGLVS 307

Query: 305 YQRYGQLRFLNKALVARMDSNKDLKTCNIVEVEEAKGILRLIPIWTASLAYAIVLSQCST 364
                Q  FLNKALVA+  S      C+I E+EEAK +LRL PIW   L YA+V +Q  T
Sbjct: 308 CSSSQQFSFLNKALVAKNGS------CSIDELEEAKSVLRLAPIWLTCLVYAVVFAQSPT 367

Query: 365 FFVKQASTMDRSISPTFKIPAATIQCFALIAVLLFLPIYDQLLVPIARIFTLKPSGISML 424
           FF KQ +TM+RSI+P +KI  AT+Q F  +++++F+PIYD++L+PIAR FT KP GI+ML
Sbjct: 368 FFTKQGATMERSITPGYKISPATLQSFISLSIVIFIPIYDRVLIPIARSFTHKPGGITML 427

Query: 425 QRIGVGMFISTLSMVVAALVECKRLAVAREHGLTHNPNATIPITIWWLTPQLILLGVSSV 484
           QRIG G+F+S L+MVVAALVE KRL  A ++GL  +P+AT+P+++WWL PQ +L G++ V
Sbjct: 428 QRIGTGIFLSFLAMVVAALVEMKRLKTAADYGLVDSPDATVPMSVWWLVPQYVLFGITDV 487

Query: 485 FTMVGLQEFFYDQVLIELKSVGLALYLSIFGVGNLLSGVLVSIIEDTTGRHGRGGWLANN 544
           F MVGLQEFFYDQV  EL+SVGLALYLSIFG+GN LS  ++SIIE  T + G+  W ANN
Sbjct: 488 FAMVGLQEFFYDQVPNELRSVGLALYLSIFGIGNFLSSFMISIIEKATSQSGQASWFANN 547

Query: 545 INNAHLDYFYWLLAGIGEVGLLAYMYFANSYVYK 579
           +N AHLDYFYWLLA +  +GL +Y+Y A SYV K
Sbjct: 548 LNQAHLDYFYWLLACLSFIGLASYLYVAKSYVSK 552

BLAST of Lsi08G000170 vs. TAIR10
Match: AT1G72140.1 (AT1G72140.1 Major facilitator superfamily protein)

HSP 1 Score: 531.9 bits (1369), Expect = 4.8e-151
Identity = 288/571 (50.44%), Postives = 380/571 (66.55%), Query Frame = 1

Query: 12  ILNDDVVPGSVDHRERPSRRSESGRWRAAGLIIGVEIAERLAFFGISTNLVSYLTVEMGQ 71
           I++++ V  SVD R  PS RS SG W+++G  +  E+AE+ A+FGI++NL++Y T  +G+
Sbjct: 13  IVSNENVEFSVDFRGNPSIRSSSGAWKSSGFTMCAEVAEKFAYFGIASNLITYFTEALGE 72

Query: 72  SMAAAAKNVNLWIGTASLLPLLAASFADSFLGRYLTILLASALYILVNLLFPFLFLILIL 131
           S A AA NVNLW+GTA+ LPL+  S ADSFLGR+ TILL S+ YI+   L  F   I  L
Sbjct: 73  STAVAASNVNLWLGTAAFLPLIWGSIADSFLGRFRTILLTSSFYIMGLGLLTFSATIPSL 132

Query: 132 LNIIIINKLSTLRGSGSAATGASSQPVPDADVVLFFSALYLVAFAQGGHKPCVQAFGCDQ 191
            N                 T  S   V    V++FF ALYL+A  +GG K C++AFG DQ
Sbjct: 133 CN--------------DQETRESC--VSQVKVIIFFCALYLIALGEGGFKVCLRAFGADQ 192

Query: 192 FDGEDPQECIAKCSFFNWWYFATTFGSFIALIILNYIQDNLGWGLGFGIPCISSVAALVV 251
           FD +DP E  AK S+FNW YFA + G     ++ NY+Q+NL W LG+ IPC+S + AL +
Sbjct: 193 FDEQDPNESKAKSSYFNWLYFAISIGILTTRLVTNYVQENLSWALGYAIPCLSMMLALFL 252

Query: 252 FLLGTRTYRFVT-----VANNEEKTFMRIGRVFVNAARNWRTTSSDIAILEEGQDAMLYQ 311
           FLLG +TYRF T          +  F+RIGRVFV AARN R T SD  +L   +    + 
Sbjct: 253 FLLGIKTYRFSTGGEGRQGKKHDNPFVRIGRVFVAAARNRRQTPSDTCLLLPNESTKKF- 312

Query: 312 RYGQLRFLNKALVARMDSNKDLKTCNIVEVEEAKGILRLIPIWTASLAYAIVLSQCSTFF 371
                RFL++A+++          C+  EVEEAK +L LIPIW  SL + IV +Q  TFF
Sbjct: 313 -----RFLDRAVIS----------CDSYEVEEAKAVLSLIPIWLCSLVFGIVFAQSPTFF 372

Query: 372 VKQASTMDRSISPTFKIPAATIQCFALIAVLLFLPIYDQLLVPIARIFTLKPSGISMLQR 431
            KQ STMDRSIS T ++PAAT+QCF  +A+L+F+PIYD+L VPIAR  T KP+GI+ LQR
Sbjct: 373 TKQGSTMDRSISSTLQVPAATLQCFISLAILVFIPIYDRLFVPIARSITRKPAGITTLQR 432

Query: 432 IGVGMFISTLSMVVAALVECKRLAVAREHGLTHNPNATIPITIWWLTPQLILLGVSSVFT 491
           I  G+F+S +SMV+AALVE KRL  AR+HGL  +P AT+P+++ WL PQ IL GVS VFT
Sbjct: 433 ISTGIFLSIISMVIAALVEMKRLKTARDHGLVDSPKATVPMSVCWLIPQYILFGVSDVFT 492

Query: 492 MVGLQEFFYDQVLIELKSVGLALYLSIFGVGNLLSGVLVSIIEDTTGRHGRGGWLANNIN 551
           MVGLQEFFY +V  +L+S+GLALYLSI G+GN LS  +VS+IE+ T + G+  W +NN+N
Sbjct: 493 MVGLQEFFYGEVPPQLRSMGLALYLSIIGIGNFLSSFMVSVIEEATSQSGQVSWFSNNLN 551

Query: 552 NAHLDYFYWLLAGIGEVGLLAYMYFANSYVY 578
            AHLDYFYWLLA +  +  +  +YFA SY+Y
Sbjct: 553 QAHLDYFYWLLACLSSLAFIFTVYFAKSYLY 551

BLAST of Lsi08G000170 vs. TAIR10
Match: AT1G72125.1 (AT1G72125.1 Major facilitator superfamily protein)

HSP 1 Score: 514.6 bits (1324), Expect = 7.9e-146
Identity = 277/567 (48.85%), Postives = 379/567 (66.84%), Query Frame = 1

Query: 13  LNDDVVPGSVDHRERPSRRSESGRWRAAGLIIGVEIAERLAFFGISTNLVSYLTVEMGQS 72
           L ++ V  +VDHR   +RRS +GRWRAA  IIGVE+AER A +GI +NL+SYLT  +GQS
Sbjct: 9   LQEEYVIDAVDHRGFSARRSITGRWRAAWFIIGVEVAERFANYGIGSNLISYLTGPLGQS 68

Query: 73  MAAAAKNVNLWIGTASLLPLLAASFADSFLGRYLTILLASALYILVNLLFPFLFLILILL 132
            A AA NVN W G +++LPLL A  AD+FLGRY+TI++AS +Y+L         L  + L
Sbjct: 69  TAVAAANVNAWSGISTILPLLGAFVADAFLGRYITIIIASFIYVLG--------LAFLTL 128

Query: 133 NIIIINKLSTLRGSGSAATGASSQPVPDADVVLFFSALYLVAFAQGGHKPCVQAFGCDQF 192
           +  +I   + +  S S+   A           LFF +LYLVA  Q GHKPCVQAFG DQF
Sbjct: 129 SAFLIPNNTEVTSSPSSFLNA-----------LFFFSLYLVAIGQSGHKPCVQAFGADQF 188

Query: 193 DGEDPQECIAKCSFFNWWYFATTFGSFIALIILNYIQDNLGWGLGFGIPCISSVAALVVF 252
           D ++PQE   + SFFNWWY +   G  +A++++ YIQ+N+ W LGFGIPC+  V +LV+F
Sbjct: 189 DEKNPQENSDRSSFFNWWYLSMCAGIGLAILVVVYIQENVSWALGFGIPCVFMVISLVLF 248

Query: 253 LLGTRTYRFVTVANNEEKT-FMRIGRVFVNAARNWRTTSSDIAILEEGQDAMLYQRYGQL 312
           +LG ++YRF      EE   F RIGRVF  A +N R  SSD+  +E  +     +   +L
Sbjct: 249 VLGRKSYRFSKTRQEEETNPFTRIGRVFFVAFKNQRLNSSDLCKVELIEANRSQESPEEL 308

Query: 313 RFLNKALVARMDSNKDLKTCNIVEVEEAKGILRLIPIWTASLAYAIVLSQCSTFFVKQAS 372
            FLNKAL+   DS++    C   +VE+A  ++RLIP+W  +LAYAI  +Q  TFF KQ  
Sbjct: 309 SFLNKALLVPNDSDEGEVACKSRDVEDATALVRLIPVWLTTLAYAIPFAQYMTFFTKQGV 368

Query: 373 TMDRSISPTFKIPAATIQCFALIAVLLFLPIYDQLLVPIARIFTLKPSGISMLQRIGVGM 432
           TM+R+I P  +IP A++Q    I+++LF+PIYD++LVPI R  T  P GI+ L+RIG GM
Sbjct: 369 TMERTIFPGVEIPPASLQVLISISIVLFVPIYDRVLVPIGRSITKDPCGITTLKRIGTGM 428

Query: 433 FISTLSMVVAALVECKRLAVAREHGLTHNPNATIPITIWWLTPQLILLGVSSVFTMVGLQ 492
            ++TL+MVVAALVE KRL  A+E+GL   P  T+P++IWWL PQ +LLG++ V T+VG+Q
Sbjct: 429 VLATLTMVVAALVESKRLETAKEYGLIDQPKTTLPMSIWWLFPQYMLLGLADVHTLVGMQ 488

Query: 493 EFFYDQVLIELKSVGLALYLSIFGVGNLLSGVLVSIIEDTTGRHGRGGWLANNINNAHLD 552
           EFFY QV  EL+S+GLA+YLS  GVG+LLS +L+ +I+  TG      W  +N+N AHLD
Sbjct: 489 EFFYSQVPTELRSLGLAIYLSAMGVGSLLSSLLIYLIDLATGGDAGNSWFNSNLNRAHLD 548

Query: 553 YFYWLLAGIGEVGLLAYMYFANSYVYK 579
           YFYWLLA +  VG   +++ + SY+Y+
Sbjct: 549 YFYWLLAVVSAVGFFTFLFISKSYIYR 556

BLAST of Lsi08G000170 vs. TAIR10
Match: AT1G72120.1 (AT1G72120.1 Major facilitator superfamily protein)

HSP 1 Score: 513.5 bits (1321), Expect = 1.8e-145
Identity = 275/567 (48.50%), Postives = 378/567 (66.67%), Query Frame = 1

Query: 13  LNDDVVPGSVDHRERPSRRSESGRWRAAGLIIGVEIAERLAFFGISTNLVSYLTVEMGQS 72
           L ++ V  +VDHR   +RRS +GRWRAA  IIGVE+AER A++GI +NL+SYLT  +G+S
Sbjct: 9   LQEEYVTDAVDHRGLAARRSNTGRWRAALFIIGVEVAERFAYYGIGSNLISYLTGPLGES 68

Query: 73  MAAAAKNVNLWIGTASLLPLLAASFADSFLGRYLTILLASALYILVNLLFPFLFLILILL 132
            A AA NVN W G A+LLP+L A  AD+FLGRY TI+++S +Y+L         L  + L
Sbjct: 69  TAVAAANVNAWSGIATLLPVLGAFVADAFLGRYRTIIISSLIYVLG--------LAFLTL 128

Query: 133 NIIIINKLSTLRGSGSAATGASSQPVPDADVVLFFSALYLVAFAQGGHKPCVQAFGCDQF 192
           +  +I   + +  S S+              VLFF +LYLVA  Q GHKPCVQAFG DQF
Sbjct: 129 SAFLIPNTTEVTSSTSSFLN-----------VLFFFSLYLVAIGQSGHKPCVQAFGADQF 188

Query: 193 DGEDPQECIAKCSFFNWWYFATTFGSFIALIILNYIQDNLGWGLGFGIPCISSVAALVVF 252
           D +D QE   + SFFNWWY + + G   A++++ YIQ+   W  GFGIPC+  V +LV+F
Sbjct: 189 DEKDSQEKSDRSSFFNWWYLSLSAGICFAILVVVYIQEEFSWAFGFGIPCVFMVISLVLF 248

Query: 253 LLGTRTYRFVTVANNEE-KTFMRIGRVFVNAARNWRTTSSDIAILEEGQDAMLYQRYGQL 312
           + G R YR+    + EE   F RIGRVF  A +N R +SSD+  +E   +    ++    
Sbjct: 249 VSGRRIYRYSKRRHEEEINPFTRIGRVFFVALKNQRLSSSDLCKVELEANTSPEKQ---- 308

Query: 313 RFLNKALVARMDSNKDLKTCNIVEVEEAKGILRLIPIWTASLAYAIVLSQCSTFFVKQAS 372
            F NKAL+   DS++        +VE+A  ++RLIP+W  +LAYAI  +Q  TFF KQ  
Sbjct: 309 SFFNKALLVPNDSSQGENASKSSDVEDATALIRLIPVWFTTLAYAIPYAQYMTFFTKQGV 368

Query: 373 TMDRSISPTFKIPAATIQCFALIAVLLFLPIYDQLLVPIARIFTLKPSGISMLQRIGVGM 432
           TMDR+I P  KIP A++Q F  I+++LF+PIYD++ VPIAR+ T +P GI+ L+RIG G+
Sbjct: 369 TMDRTILPGVKIPPASLQVFIGISIVLFVPIYDRVFVPIARLITKEPCGITTLKRIGTGI 428

Query: 433 FISTLSMVVAALVECKRLAVAREHGLTHNPNATIPITIWWLTPQLILLGVSSVFTMVGLQ 492
            +ST++MV+AALVE KRL  A+EHGL   P AT+P++IWWL PQ +LLG++ V+T+VG+Q
Sbjct: 429 VLSTITMVIAALVEFKRLETAKEHGLIDQPEATLPMSIWWLIPQYLLLGLADVYTLVGMQ 488

Query: 493 EFFYDQVLIELKSVGLALYLSIFGVGNLLSGVLVSIIEDTTGRHGRGGWLANNINNAHLD 552
           EFFY QV  EL+S+GLALYLS  GVG+LLS +L+S+I+  TG      W  +N+N AHLD
Sbjct: 489 EFFYSQVPTELRSIGLALYLSALGVGSLLSSLLISLIDLATGGDAGNSWFNSNLNRAHLD 548

Query: 553 YFYWLLAGIGEVGLLAYMYFANSYVYK 579
           YFYWLLA +  VG   +++ + SY+Y+
Sbjct: 549 YFYWLLAIVSAVGFFTFLFISKSYIYR 552

BLAST of Lsi08G000170 vs. TAIR10
Match: AT1G22550.1 (AT1G22550.1 Major facilitator superfamily protein)

HSP 1 Score: 502.3 bits (1292), Expect = 4.1e-142
Identity = 276/568 (48.59%), Postives = 380/568 (66.90%), Query Frame = 1

Query: 13  LNDDVVPGSVDHRERPSRRSESGRWRAAGLIIGVEIAERLAFFGISTNLVSYLTVEMGQS 72
           L +D V  SVDHR  P+ +S +G WR+A  IIGVE+ ER A+FGI +NL++YLT  +GQS
Sbjct: 10  LIEDSVSDSVDHRGLPAGKSSTGGWRSAWYIIGVEVGERFAYFGIGSNLITYLTGPLGQS 69

Query: 73  MAAAAKNVNLWIGTASLLPLLAASFADSFLGRYLTILLASALYILVNLLFPFLFLILILL 132
            A AA NVN W GTAS+LP+L A  AD++LGRY TI++AS +YIL          +L L 
Sbjct: 70  TATAAVNVNTWSGTASILPVLGAFIADAYLGRYRTIVVASLIYILG-------LGLLTLS 129

Query: 133 NIIIINKLSTLRGSGSAATGASSQPVPDADVVLFFSALYLVAFAQGGHKPCVQAFGCDQF 192
           +I+I+  LS  R        AS++P    ++ LFF +LYLVA  QGGHKPCVQAFG DQF
Sbjct: 130 SILILMGLSEQRQHNR---NASAKPFFWVNI-LFFCSLYLVAIGQGGHKPCVQAFGADQF 189

Query: 193 DGEDPQECIAKCSFFNWWYFATTFGSFIALIILNYIQDNLGWGLGFGIPCISSVAALVVF 252
           D  DP+E I++ SFFNWW+ + + G  +++I++ Y+QDN+ W LGFGIPC+  V AL +F
Sbjct: 190 DVGDPKERISRGSFFNWWFLSLSAGITLSIIVVVYVQDNVNWALGFGIPCLFMVMALALF 249

Query: 253 LLGTRTYRFVTV-ANNEEKTFMRIGRVFVNAARNWRTTSSDIAILEEGQDAMLYQRY-GQ 312
           L G +TYR+       +   F RIGRVF+ A +N +   +    LE G     Y++  GQ
Sbjct: 250 LFGRKTYRYPRGDREGKNNAFARIGRVFLVAFKNRKLKLTHSGQLEVGS----YKKCKGQ 309

Query: 313 LRFLNKALVARMDSNKDLKTCNIVEVEEAKGILRLIPIWTASLAYAIVLSQCSTFFVKQA 372
           L FL KAL   +     ++ C+  +VE+A  ++RLIPIW  S+   I  +Q +TFF KQ 
Sbjct: 310 LEFLAKAL---LPGEGGVEPCSSRDVEDAMALVRLIPIWITSVVSTIPYAQYATFFTKQG 369

Query: 373 STMDRSISPTFKIPAATIQCFALIAVLLFLPIYDQLLVPIARIFTLKPSGISMLQRIGVG 432
            T+DR I P F+IP A+ Q    +++ + +P Y+++ +P+AR+ T KPSGI+MLQRIG G
Sbjct: 370 VTVDRKILPGFEIPPASFQALIGLSIFISVPTYERVFLPLARLITKKPSGITMLQRIGAG 429

Query: 433 MFISTLSMVVAALVECKRLAVAREHGLTHNPNATIPITIWWLTPQLILLGVSSVFTMVGL 492
           M +S+L+MVVAALVE KRL  A+EHGL   P+ATIP++IWW  PQ +LLG+  VF++VG 
Sbjct: 430 MVLSSLNMVVAALVEMKRLETAKEHGLVDRPDATIPMSIWWFVPQYLLLGMIDVFSLVGT 489

Query: 493 QEFFYDQVLIELKSVGLALYLSIFGVGNLLSGVLVSIIEDTTGRHGRGGWLANNINNAHL 552
           QEFFYDQV  EL+S+GLAL LS  G+ + LSG L+++I   TG++G   W   N+N AH+
Sbjct: 490 QEFFYDQVPTELRSIGLALSLSAMGLASFLSGFLITVINWATGKNGGDSWFNTNLNRAHV 549

Query: 553 DYFYWLLAGIGEVGLLAYMYFANSYVYK 579
           DYFYWLLA    +G LA++  +  YVY+
Sbjct: 550 DYFYWLLAAFTAIGFLAFLLLSRLYVYR 559

BLAST of Lsi08G000170 vs. NCBI nr
Match: gi|778708109|ref|XP_011656123.1| (PREDICTED: protein NRT1/ PTR FAMILY 5.10-like [Cucumis sativus])

HSP 1 Score: 883.2 bits (2281), Expect = 2.4e-253
Identity = 464/557 (83.30%), Postives = 504/557 (90.48%), Query Frame = 1

Query: 29  SRRSESGRWRAAGLIIGVEIAERLAFFGISTNLVSYLTVEMGQSMAAAAKNVNLWIGTAS 88
           +RRS+SG WRAAG+IIGVEIAER AFFGISTNLVSYLTVEMGQSMA AA+NVNLW+GTAS
Sbjct: 53  NRRSQSGGWRAAGVIIGVEIAERFAFFGISTNLVSYLTVEMGQSMADAAQNVNLWVGTAS 112

Query: 89  LLPLLAASFADSFLGRYLTILLASALYILVNLLFPFLFLILILLNIIIINKLSTLRGSGS 148
           LLPLLAASFADSFLGRYLTI+LASALYIL         L L+ L+ I+ +  S+ +GSGS
Sbjct: 113 LLPLLAASFADSFLGRYLTIILASALYILG--------LGLLTLSAILASP-SSFQGSGS 172

Query: 149 AATGASSQPVPDADVVLFFSALYLVAFAQGGHKPCVQAFGCDQFDGEDPQECIAKCSFFN 208
           AA+GASS+PV    V+ FF++LYLVAFAQGGHKPC+QAFGCDQFDGEDPQECIAKCSFFN
Sbjct: 173 AASGASSRPV--LHVLFFFTSLYLVAFAQGGHKPCLQAFGCDQFDGEDPQECIAKCSFFN 232

Query: 209 WWYFATTFGSFIALIILNYIQDNLGWGLGFGIPCISSVAALVVFLLGTRTYRFVTVANNE 268
           WWYF+TT GSFIALIIL+YIQDNLGWGLGFGIPCISS+ AL+VFLLGT TYRF  +AN+E
Sbjct: 233 WWYFSTTLGSFIALIILSYIQDNLGWGLGFGIPCISSLVALLVFLLGTHTYRFGKIANDE 292

Query: 269 EKTFMRIGRVFVNAARNWRTTSSDIAILEEGQDAMLYQRYGQLRFLNKALVARMDSNKDL 328
           EK FMRIGRVF NAARNWRT SS+I ILEEGQDAMLYQR GQLRFLNKALVA MDS++D 
Sbjct: 293 EKPFMRIGRVFFNAARNWRTKSSEIDILEEGQDAMLYQRSGQLRFLNKALVAPMDSDEDG 352

Query: 329 KTCNIVEVEEAKGILRLIPIWTASLAYAIVLSQCSTFFVKQASTMDRSISPTFKIPAATI 388
            TCNIVEVEEAKGILRLIPIW ASL+YAIVLSQCSTFFVKQ +TMDRSI+P+FKIPAATI
Sbjct: 353 NTCNIVEVEEAKGILRLIPIWIASLSYAIVLSQCSTFFVKQGATMDRSITPSFKIPAATI 412

Query: 389 QCFALIAVLLFLPIYDQLLVPIARIFTLKPSGISMLQRIGVGMFISTLSMVVAALVECKR 448
           QCF  IAV+ F+PIYD+LLVPIARIFTLKPSGISMLQRIGVGMFISTLSMVVAALVE KR
Sbjct: 413 QCFGCIAVVFFVPIYDRLLVPIARIFTLKPSGISMLQRIGVGMFISTLSMVVAALVEVKR 472

Query: 449 LAVAREHGLTHNPNATIPITIWWLTPQLILLGVSSVFTMVGLQEFFYDQVLIELKSVGLA 508
           LAVAR HGLT NPN+TIPITIWWLTPQL+LLGVSSVFTMVGLQEFFYDQV  E+KSVGLA
Sbjct: 473 LAVARAHGLTQNPNSTIPITIWWLTPQLLLLGVSSVFTMVGLQEFFYDQVSSEVKSVGLA 532

Query: 509 LYLSIFGVGNLLSGVLVSIIEDTT-GRHGRGGWLANNINNAHLDYFYWLLAGIGEVGLLA 568
           LYLSIFGVGNLLSG+LVS+IED T G  GRGGW ANNIN AHLDYFYWLLAGIG+VGLLA
Sbjct: 533 LYLSIFGVGNLLSGILVSVIEDATGGDDGRGGWFANNINTAHLDYFYWLLAGIGKVGLLA 592

Query: 569 YMYFANSYVYKYNVERS 585
           YMYFANSYVYKYNVERS
Sbjct: 593 YMYFANSYVYKYNVERS 598

BLAST of Lsi08G000170 vs. NCBI nr
Match: gi|659119563|ref|XP_008459724.1| (PREDICTED: uncharacterized protein LOC103498766 [Cucumis melo])

HSP 1 Score: 875.9 bits (2262), Expect = 3.8e-251
Identity = 469/576 (81.42%), Postives = 512/576 (88.89%), Query Frame = 1

Query: 12   ILNDDVVPGSVDHRERPSRRSESGRWRAAGLIIGVEIAERLAFFGISTNLVSYLTVEMGQ 71
            ILND  +PG+        RRS+SG WRAAG+IIGVEIAER AFFGISTNLVSYLTVEM Q
Sbjct: 614  ILND-AIPGN--------RRSQSGGWRAAGVIIGVEIAERFAFFGISTNLVSYLTVEMEQ 673

Query: 72   SMAAAAKNVNLWIGTASLLPLLAASFADSFLGRYLTILLASALYILVNLLFPFLFLILIL 131
            SMA+AA+NVNLWIGTASLLPL+AASFADSFLGRYLTI+LASALYIL         L L+ 
Sbjct: 674  SMASAAQNVNLWIGTASLLPLVAASFADSFLGRYLTIILASALYILG--------LGLLT 733

Query: 132  LNIIIINKLSTLRGSGSAA---TGASSQPVPDADVVLFFSALYLVAFAQGGHKPCVQAFG 191
            L+ ++ +  S+ +GSGSAA   TGAS++PV    VVLFFS+LYLVAFAQGGHKPC+QAFG
Sbjct: 734  LSAMLASP-SSFQGSGSAASAATGASARPV--LHVVLFFSSLYLVAFAQGGHKPCLQAFG 793

Query: 192  CDQFDGEDPQECIAKCSFFNWWYFATTFGSFIALIILNYIQDNLGWGLGFGIPCISSVAA 251
            CDQFDGEDPQECIAKCSFFNWWYFATT GSFIALIIL+YIQDNLGW LGFGIPCISS+ A
Sbjct: 794  CDQFDGEDPQECIAKCSFFNWWYFATTLGSFIALIILSYIQDNLGWCLGFGIPCISSLVA 853

Query: 252  LVVFLLGTRTYRFVTVANNEEKTFMRIGRVFVNAARNWRTTSSDIAILEEGQDAMLYQRY 311
            L+VFLLGT+TYRFVT+AN+EEK FMRIGRVF NAARNWRT SS+I I EEGQDAMLY+R 
Sbjct: 854  LLVFLLGTQTYRFVTIANDEEKPFMRIGRVFFNAARNWRTASSEIDISEEGQDAMLYRRC 913

Query: 312  GQLRFLNKALVARMDSNKDLKTCNIVEVEEAKGILRLIPIWTASLAYAIVLSQCSTFFVK 371
            GQLRFLNKALVA +DS+ D KTCNIVEVEEAKG+LRLIPIW ASL+YAIVLSQCSTFFVK
Sbjct: 914  GQLRFLNKALVAPVDSDDDGKTCNIVEVEEAKGVLRLIPIWIASLSYAIVLSQCSTFFVK 973

Query: 372  QASTMDRSISPTFKIPAATIQCFALIAVLLFLPIYDQLLVPIARIFTLKPSGISMLQRIG 431
            Q STMDRSI P+FKIPAATIQCF  IAV+LF+PIYD+LLVPIARIFTLKPSGISMLQRIG
Sbjct: 974  QGSTMDRSIRPSFKIPAATIQCFGCIAVVLFVPIYDRLLVPIARIFTLKPSGISMLQRIG 1033

Query: 432  VGMFISTLSMVVAALVECKRLAVAREHGLTHNPNATIPITIWWLTPQLILLGVSSVFTMV 491
            VGMFISTLSMVVAALVE KRLAVAREHGLT NPN+TIPITIWWLTPQL+LLGVSSVFTMV
Sbjct: 1034 VGMFISTLSMVVAALVEVKRLAVAREHGLTQNPNSTIPITIWWLTPQLLLLGVSSVFTMV 1093

Query: 492  GLQEFFYDQVLIELKSVGLALYLSIFGVGNLLSGVLVSIIEDTT-GRHGRGGWLANNINN 551
            GLQEFFYDQV  E+KSVGLALYLSIFGVGNLLS +LVS+IE+ T G  GRGGW ANNIN 
Sbjct: 1094 GLQEFFYDQVSSEVKSVGLALYLSIFGVGNLLSSLLVSVIEEATGGDDGRGGWFANNINK 1153

Query: 552  AHLDYFYWLLAGIGEVGLLAYMYFANSYVYKYNVER 584
            AHLDYFYWLLAGIG+VGLLAY+YFANSYVYKYNVER
Sbjct: 1154 AHLDYFYWLLAGIGKVGLLAYVYFANSYVYKYNVER 1169

BLAST of Lsi08G000170 vs. NCBI nr
Match: gi|566200617|ref|XP_006376225.1| (hypothetical protein POPTR_0013s11120g [Populus trichocarpa])

HSP 1 Score: 646.7 bits (1667), Expect = 3.8e-182
Identity = 340/580 (58.62%), Postives = 426/580 (73.45%), Query Frame = 1

Query: 3   MADESRRPLILNDDVVPGSVDHRERPSRRSESGRWRAAGLIIGVEIAERLAFFGISTNLV 62
           M+  ++ PL L+ D+V GSVD++ RP+ RS SG WR+A  IIGVE+AER A++GIS+NL+
Sbjct: 1   MSSSNQTPL-LSYDIVDGSVDYKSRPAYRSNSGGWRSASFIIGVEVAERFAYYGISSNLI 60

Query: 63  SYLTVEMGQSMAAAAKNVNLWIGTASLLPLLAASFADSFLGRYLTILLASALYIL-VNLL 122
           +YLT  +GQS A AA+NVN+W GTASLLPLL A  ADSFLGRY TI+ AS +YIL + LL
Sbjct: 61  TYLTGPLGQSTATAAENVNVWAGTASLLPLLGAFVADSFLGRYRTIIFASLIYILGLGLL 120

Query: 123 FPFLFLILILLNIIIINKLSTLRGSGSAATGASSQPVPDADV----VLFFSALYLVAFAQ 182
                             LS L  S +AATG  S  +P + V    +LFF ALYLVA  Q
Sbjct: 121 -----------------TLSALLPSHAAATGFRSTNLPSSPVPFQLILFFFALYLVAIGQ 180

Query: 183 GGHKPCVQAFGCDQFDGEDPQECIAKCSFFNWWYFATTFGSFIALIILNYIQDNLGWGLG 242
            GHKPCVQAFG DQFDG+DPQE  AK SFFNWWY     G+ + L++LNYIQDNL WGLG
Sbjct: 181 SGHKPCVQAFGADQFDGQDPQESKAKSSFFNWWYCFMCAGTVVTLLVLNYIQDNLNWGLG 240

Query: 243 FGIPCISSVAALVVFLLGTRTYRFVTVANNEEKTFMRIGRVFVNAARNWRTTSSDIAILE 302
           FGIPC   V AL++FLLGT+TYR+ ++  +E+  F+RIG VFV++ RNWRTT S IA  E
Sbjct: 241 FGIPCAVMVIALIIFLLGTKTYRY-SIKGDEKSAFLRIGWVFVSSIRNWRTTPSAIAFEE 300

Query: 303 EGQDAMLYQRYGQLRFLNKALVARMDSNKDLKTCNIVEVEEAKGILRLIPIWTASLAYAI 362
           E +  + +Q   Q +FLNKAL+A     +D K C++ +VEEAK +LRL+PIW   L +AI
Sbjct: 301 ETRGTLPHQSSEQYKFLNKALLAPNGLKEDGKVCSLGDVEEAKALLRLVPIWATCLVFAI 360

Query: 363 VLSQCSTFFVKQASTMDRSISPTFKIPAATIQCFALIAVLLFLPIYDQLLVPIARIFTLK 422
           V +Q STFF KQ  TMDRSISP+  +PAA++Q F  ++++LF+PIYD++LVPIAR  T K
Sbjct: 361 VFAQTSTFFTKQGVTMDRSISPSLDLPAASLQSFISLSIVLFIPIYDRVLVPIARALTRK 420

Query: 423 PSGISMLQRIGVGMFISTLSMVVAALVECKRLAVAREHGLTHNPNATIPITIWWLTPQLI 482
           PSGI+MLQRIG GMFIS ++M+VAALVE KRL  A+EHGL   PN TIP+++WWL PQ +
Sbjct: 421 PSGITMLQRIGSGMFISVVAMIVAALVEMKRLKTAQEHGLVDLPNVTIPMSVWWLIPQYV 480

Query: 483 LLGVSSVFTMVGLQEFFYDQVLIELKSVGLALYLSIFGVGNLLSGVLVSIIEDTTGRHGR 542
           L GV+  FTMVGLQEFFYDQV  +L+SVGL+LYLSIFGVG+ LS  L+SIIE  TG +GR
Sbjct: 481 LFGVAEAFTMVGLQEFFYDQVPSDLRSVGLSLYLSIFGVGSFLSSFLISIIEKATGGNGR 540

Query: 543 GGWLANNINNAHLDYFYWLLAGIGEVGLLAYMYFANSYVY 578
             W ANN+N AHLDYFYWLLAG+  V L+AY+ FA SY+Y
Sbjct: 541 YSWFANNLNRAHLDYFYWLLAGLSAVQLVAYVCFAKSYIY 561

BLAST of Lsi08G000170 vs. NCBI nr
Match: gi|645270701|ref|XP_008240575.1| (PREDICTED: protein NRT1/ PTR FAMILY 5.10-like [Prunus mume])

HSP 1 Score: 641.0 bits (1652), Expect = 2.1e-180
Identity = 335/565 (59.29%), Postives = 412/565 (72.92%), Query Frame = 1

Query: 13  LNDDVVPGSVDHRERPSRRSESGRWRAAGLIIGVEIAERLAFFGISTNLVSYLTVEMGQS 72
           L DDVV G+VD++ RP  RS SG WR+A  IIGVE+AER A++GIS NL+++LT  +GQS
Sbjct: 15  LLDDVVEGAVDYKGRPVHRSRSGGWRSAWFIIGVEVAERFAYYGISCNLITFLTGPLGQS 74

Query: 73  MAAAAKNVNLWIGTASLLPLLAASFADSFLGRYLTILLASALYILVNLLFPFLFLILILL 132
            A AA+NVN+W GTASLLPLL A  ADSFLGRY TI+ AS LYIL         L L+ L
Sbjct: 75  TATAAENVNIWSGTASLLPLLGAFVADSFLGRYRTIVFASLLYILG--------LGLLTL 134

Query: 133 NIIIINKLSTLRGSGSAATGASSQPVPDADVVLFFSALYLVAFAQGGHKPCVQAFGCDQF 192
           + +    L +L GS       S+       V+ FF +LYLVA AQGGHKPCVQAFG DQF
Sbjct: 135 SAV----LPSLTGSDCQKINQSTSCSSQFQVLFFFFSLYLVAVAQGGHKPCVQAFGADQF 194

Query: 193 DGEDPQECIAKCSFFNWWYFATTFGSFIALIILNYIQDNLGWGLGFGIPCISSVAALVVF 252
           D  DP+EC AK SFFNWWYF+  FG+   LI+L YIQDNL WGLGFGIPCI+ V AL +F
Sbjct: 195 DASDPEECKAKSSFFNWWYFSLCFGTTFTLILLTYIQDNLSWGLGFGIPCIAMVLALFIF 254

Query: 253 LLGTRTYRFVTVANNEEKTFMRIGRVFVNAARNWRTTSSDIAILEEGQDAMLYQRYGQLR 312
           LLGTRTYR+ ++  +EE  F+RIGRVFV A RNWRTT S I   EE +  + ++   Q +
Sbjct: 255 LLGTRTYRY-SIKGDEESPFVRIGRVFVTALRNWRTTPSAITSEEESRGTLPHESSEQFK 314

Query: 313 FLNKALVARMDSNKDLKTCNIVEVEEAKGILRLIPIWTASLAYAIVLSQCSTFFVKQAST 372
           FLNKAL+A  D  ++ K C + EVEEAK +LRL PIW   LAYA+V +Q STFF KQ +T
Sbjct: 315 FLNKALLAPDDLKENRKVCTVAEVEEAKSVLRLFPIWVTCLAYAVVFAQYSTFFTKQGAT 374

Query: 373 MDRSISPTFKIPAATIQCFALIAVLLFLPIYDQLLVPIARIFTLKPSGISMLQRIGVGMF 432
           MDR+I+P   IPAA++Q F  I +++F+PIYD++ VPIAR+FT KPSGI+MLQRIG+GMF
Sbjct: 375 MDRTIAPGLDIPAASLQTFISITIVIFVPIYDRIFVPIARVFTRKPSGITMLQRIGIGMF 434

Query: 433 ISTLSMVVAALVECKRLAVAREHGLTHNPNATIPITIWWLTPQLILLGVSSVFTMVGLQE 492
           IS +SM VAALVE KRL  A+++GL   P+AT+P++IWWL PQ +L G++ VFTMVGLQE
Sbjct: 435 ISIISMAVAALVEMKRLKTAKDYGLLDMPSATVPMSIWWLVPQYLLAGLADVFTMVGLQE 494

Query: 493 FFYDQVLIELKSVGLALYLSIFGVGNLLSGVLVSIIEDTTGRHGRGGWLANNINNAHLDY 552
           FFYDQV  ELKS+GLALYLSIFGVG+ LS  L+S I+D T   G   W + N+N AHLDY
Sbjct: 495 FFYDQVPNELKSIGLALYLSIFGVGSFLSSFLISAIDDATTWAGETSWFSTNLNRAHLDY 554

Query: 553 FYWLLAGIGEVGLLAYMYFANSYVY 578
           FYWLL GI  V L  Y+YFA SY+Y
Sbjct: 555 FYWLLGGISVVELAIYLYFAKSYIY 566

BLAST of Lsi08G000170 vs. NCBI nr
Match: gi|743914303|ref|XP_011001076.1| (PREDICTED: protein NRT1/ PTR FAMILY 5.10-like [Populus euphratica])

HSP 1 Score: 639.0 bits (1647), Expect = 7.9e-180
Identity = 339/580 (58.45%), Postives = 420/580 (72.41%), Query Frame = 1

Query: 3   MADESRRPLILNDDVVPGSVDHRERPSRRSESGRWRAAGLIIGVEIAERLAFFGISTNLV 62
           M+  ++ PL L+ D+V GSVD + RP+ RS SG WR+A  IIGVE+AER A++GIS+NL+
Sbjct: 1   MSSFNQTPL-LSYDIVDGSVDSKGRPAYRSNSGGWRSASFIIGVEVAERFAYYGISSNLI 60

Query: 63  SYLTVEMGQSMAAAAKNVNLWIGTASLLPLLAASFADSFLGRYLTILLASALYIL-VNLL 122
           +YLT  +GQS A AA+NVN+W GT SLLPLL A  ADSFLGRY TI+ AS +YIL + LL
Sbjct: 61  TYLTGPLGQSTATAAENVNVWAGTVSLLPLLGAFVADSFLGRYRTIIFASLIYILGLGLL 120

Query: 123 FPFLFLILILLNIIIINKLSTLRGSGSAATGASSQPVPDADV----VLFFSALYLVAFAQ 182
                             LS L  SG+A  G  S  +P + V    +LFF ALYLVA  Q
Sbjct: 121 -----------------TLSALLPSGAAVAGFRSTDLPSSPVPFQLILFFFALYLVAVGQ 180

Query: 183 GGHKPCVQAFGCDQFDGEDPQECIAKCSFFNWWYFATTFGSFIALIILNYIQDNLGWGLG 242
            GHKPCVQAFG DQFDG+DP E  AK SFFNWWY     G+ I L++LNYIQDNL WGLG
Sbjct: 181 SGHKPCVQAFGADQFDGQDPLESKAKSSFFNWWYCFMCAGTVITLLVLNYIQDNLNWGLG 240

Query: 243 FGIPCISSVAALVVFLLGTRTYRFVTVANNEEKTFMRIGRVFVNAARNWRTTSSDIAILE 302
           FGIPC   V ALV+FLLGTRTYR+ ++  +E+  F+RIG VFV++ RNWRTT S IA  E
Sbjct: 241 FGIPCAVMVIALVIFLLGTRTYRY-SIKGDEKSAFLRIGWVFVSSIRNWRTTPSAIAFEE 300

Query: 303 EGQDAMLYQRYGQLRFLNKALVARMDSNKDLKTCNIVEVEEAKGILRLIPIWTASLAYAI 362
           E +  + +Q   Q +FLNKAL+A   S +D K C + +VEEAK +LRL+PIW   L +AI
Sbjct: 301 ETRGTLPHQSSEQYKFLNKALLAPNGSKEDGKVCTLGDVEEAKALLRLVPIWATCLVFAI 360

Query: 363 VLSQCSTFFVKQASTMDRSISPTFKIPAATIQCFALIAVLLFLPIYDQLLVPIARIFTLK 422
           V +Q STFF KQ  TMDRSISP+  +PAA++Q F  ++++LF+PIYD++LVPIAR  T K
Sbjct: 361 VFAQTSTFFTKQGVTMDRSISPSLDLPAASLQSFISLSIVLFIPIYDRVLVPIARALTRK 420

Query: 423 PSGISMLQRIGVGMFISTLSMVVAALVECKRLAVAREHGLTHNPNATIPITIWWLTPQLI 482
           PSGI+MLQRIG GMFIS ++M+VAALVE KRL  A+EHGL   PN TIP+++WWL PQ +
Sbjct: 421 PSGITMLQRIGSGMFISVVAMIVAALVEMKRLKTAQEHGLVDLPNVTIPMSVWWLIPQYV 480

Query: 483 LLGVSSVFTMVGLQEFFYDQVLIELKSVGLALYLSIFGVGNLLSGVLVSIIEDTTGRHGR 542
           L GV+  FTMVGLQEFFYDQV  +L+SVGL+LYLSIFGVG+ LS  L+SIIE  TG  G+
Sbjct: 481 LFGVAEAFTMVGLQEFFYDQVPSDLRSVGLSLYLSIFGVGSFLSSFLISIIEKATGGDGQ 540

Query: 543 GGWLANNINNAHLDYFYWLLAGIGEVGLLAYMYFANSYVY 578
             W ANN+N AHLDYFYWLLAG+  V L+ Y+ FA SY+Y
Sbjct: 541 YSWFANNLNRAHLDYFYWLLAGLSAVQLVVYVCFAKSYIY 561

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PTR9_ARATH4.6e-17255.75Protein NRT1/ PTR FAMILY 5.10 OS=Arabidopsis thaliana GN=NPF5.10 PE=2 SV=1[more]
PTR25_ARATH8.5e-15050.44Protein NRT1/ PTR FAMILY 5.12 OS=Arabidopsis thaliana GN=NPF5.12 PE=2 SV=1[more]
PTR23_ARATH1.4e-14448.85Protein NRT1/ PTR FAMILY 5.13 OS=Arabidopsis thaliana GN=NPF5.13 PE=2 SV=2[more]
PTR22_ARATH3.1e-14448.50Protein NRT1/ PTR FAMILY 5.14 OS=Arabidopsis thaliana GN=NPF5.14 PE=2 SV=2[more]
PTR10_ARATH7.2e-14148.59Protein NRT1/ PTR FAMILY 5.16 OS=Arabidopsis thaliana GN=NPF5.16 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
U5FXL3_POPTR2.6e-18258.62Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0013s11120g PE=3 SV=1[more]
A0A059BVA5_EUCGR1.6e-17957.84Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_F02994 PE=3 SV=1[more]
M5VYB7_PRUPE6.1e-17959.12Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa003472mg PE=3 SV=1[more]
A0A022Q2D5_ERYGU4.0e-17857.75Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a003626mg PE=4 SV=1[more]
M1D3I7_SOLTU3.3e-17757.30Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG401031322 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G22540.12.6e-17355.75 Major facilitator superfamily protein[more]
AT1G72140.14.8e-15150.44 Major facilitator superfamily protein[more]
AT1G72125.17.9e-14648.85 Major facilitator superfamily protein[more]
AT1G72120.11.8e-14548.50 Major facilitator superfamily protein[more]
AT1G22550.14.1e-14248.59 Major facilitator superfamily protein[more]
Match NameE-valueIdentityDescription
gi|778708109|ref|XP_011656123.1|2.4e-25383.30PREDICTED: protein NRT1/ PTR FAMILY 5.10-like [Cucumis sativus][more]
gi|659119563|ref|XP_008459724.1|3.8e-25181.42PREDICTED: uncharacterized protein LOC103498766 [Cucumis melo][more]
gi|566200617|ref|XP_006376225.1|3.8e-18258.62hypothetical protein POPTR_0013s11120g [Populus trichocarpa][more]
gi|645270701|ref|XP_008240575.1|2.1e-18059.29PREDICTED: protein NRT1/ PTR FAMILY 5.10-like [Prunus mume][more]
gi|743914303|ref|XP_011001076.1|7.9e-18058.45PREDICTED: protein NRT1/ PTR FAMILY 5.10-like [Populus euphratica][more]
The following terms have been associated with this gene:
Vocabulary: Cellular Component
TermDefinition
GO:0016020membrane
Vocabulary: Biological Process
TermDefinition
GO:0006810transport
Vocabulary: Molecular Function
TermDefinition
GO:0005215transporter activity
Vocabulary: INTERPRO
TermDefinition
IPR020846MFS_dom
IPR000109POT_fam
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006810 transport
biological_process GO:0006857 oligopeptide transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016020 membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005215 transporter activity
molecular_function GO:0022857 transmembrane transporter activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi08G000170.1Lsi08G000170.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 1..586
score: 1.8E
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 107..536
score: 3.1
IPR020846Major facilitator superfamily domainunknownSSF103473MFS general substrate transportercoord: 336..572
score: 2.62E-20coord: 23..125
score: 2.62E-20coord: 157..282
score: 2.62
NoneNo IPR availableGENE3DG3DSA:1.20.1250.20coord: 166..257
score: 3.4E-4coord: 51..131
score: 3.
NoneNo IPR availablePANTHERPTHR11654:SF94PROTEIN NRT1/ PTR FAMILY 5.10-RELATEDcoord: 1..586
score: 1.8E

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Lsi08G000170MELO3C022223.2Melon (DHL92) v3.6.1lsimedB479
Lsi08G000170CsGy5G030760Cucumber (Gy14) v2cgyblsiB358
Lsi08G000170CsaV3_5G040490Cucumber (Chinese Long) v3cuclsiB414
The following gene(s) are paralogous to this gene:

None