Lsi07G009140 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi07G009140
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionRING/U-box superfamily protein, putative
Locationchr07 : 10640254 .. 10640961 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTTTCTCTACTACTTCTTCCATTCAATACTGTTTTCGCGGCATTCCAAACAACGAACAAATCCTTGAAATTAGATTTGGGTATTGCAGCCGCCTGATATCTCGATCTCCCTCCCCTGAAATTATTCCAGATTTAATACTGCAACAGACTCCTTCTACTCTGATCGGGGAGACTCTGTTTTCTTTTTCCAATCTGCAGGATCGTCAACAACAACAAGCAATAAAATTTGTAGTGAATGGGTTTCTCCTTTCCTTCAACCTCCATGTCTCTGTCTGCCAAATCATCTCCCGCAAGATTCTGTCATTTGGCCAACAGTTTGACACCAACAATGGAAGCTTCAAAATAGAAGCCAACATTGATTACATGAAGGAGTTTTGGATGGAGGCTGTCCCCGGGCCATACCCGATCTCCATCATCGGAGAGGAAGAGTTTGGGAGTATTTTTGAAGAAGCTTCACCACCGGGCAGGAGAGCAGGGGCGCCGGAGTCGGCGATTGAGAGATTAAGAAAGACAAAATTTGATGGGTTTAATGATGAAGAAGAAGAAGAGGTGGGAGATTGCAGCGTGTGTTACGAAGATCTAAAAAGCAAGGGGAAAGAGGTTAGCAGGATTCCCTGCGGTCATCTATATCATAAATCGTGCATCCTCACCTGGCTTGAAAGGAGCAATTCCTGTCCATTGTGCAGAGCGTCATTAAATCCATGA

mRNA sequence

ATGTCTTTCTCTACTACTTCTTCCATTCAATACTGTTTTCGCGGCATTCCAAACAACGAACAAATCCTTGAAATTAGATTTGGGTATTGCAGCCGCCTGATATCTCGATCTCCCTCCCCTGAAATTATTCCAGATTTAATACTGCAACAGACTCCTTCTACTCTGATCGGGGAGACTCTGTTTTCTTTTTCCAATCTGCAGGATCGTCAACAACAACAAGCAATAAAATTTGTAGTGAATGGGTTTCTCCTTTCCTTCAACCTCCATGTCTCTGTCTGCCAAATCATCTCCCGCAAGATTCTGTCATTTGGCCAACAGTTTGACACCAACAATGGAAGCTTCAAAATAGAAGCCAACATTGATTACATGAAGGAGTTTTGGATGGAGGCTGTCCCCGGGCCATACCCGATCTCCATCATCGGAGAGGAAGAGTTTGGGAGTATTTTTGAAGAAGCTTCACCACCGGGCAGGAGAGCAGGGGCGCCGGAGTCGGCGATTGAGAGATTAAGAAAGACAAAATTTGATGGGTTTAATGATGAAGAAGAAGAAGAGGTGGGAGATTGCAGCGTGTGTTACGAAGATCTAAAAAGCAAGGGGAAAGAGGTTAGCAGGATTCCCTGCGGTCATCTATATCATAAATCGTGCATCCTCACCTGGCTTGAAAGGAGCAATTCCTGTCCATTGTGCAGAGCGTCATTAAATCCATGA

Coding sequence (CDS)

ATGTCTTTCTCTACTACTTCTTCCATTCAATACTGTTTTCGCGGCATTCCAAACAACGAACAAATCCTTGAAATTAGATTTGGGTATTGCAGCCGCCTGATATCTCGATCTCCCTCCCCTGAAATTATTCCAGATTTAATACTGCAACAGACTCCTTCTACTCTGATCGGGGAGACTCTGTTTTCTTTTTCCAATCTGCAGGATCGTCAACAACAACAAGCAATAAAATTTGTAGTGAATGGGTTTCTCCTTTCCTTCAACCTCCATGTCTCTGTCTGCCAAATCATCTCCCGCAAGATTCTGTCATTTGGCCAACAGTTTGACACCAACAATGGAAGCTTCAAAATAGAAGCCAACATTGATTACATGAAGGAGTTTTGGATGGAGGCTGTCCCCGGGCCATACCCGATCTCCATCATCGGAGAGGAAGAGTTTGGGAGTATTTTTGAAGAAGCTTCACCACCGGGCAGGAGAGCAGGGGCGCCGGAGTCGGCGATTGAGAGATTAAGAAAGACAAAATTTGATGGGTTTAATGATGAAGAAGAAGAAGAGGTGGGAGATTGCAGCGTGTGTTACGAAGATCTAAAAAGCAAGGGGAAAGAGGTTAGCAGGATTCCCTGCGGTCATCTATATCATAAATCGTGCATCCTCACCTGGCTTGAAAGGAGCAATTCCTGTCCATTGTGCAGAGCGTCATTAAATCCATGA

Protein sequence

MSFSTTSSIQYCFRGIPNNEQILEIRFGYCSRLISRSPSPEIIPDLILQQTPSTLIGETLFSFSNLQDRQQQQAIKFVVNGFLLSFNLHVSVCQIISRKILSFGQQFDTNNGSFKIEANIDYMKEFWMEAVPGPYPISIIGEEEFGSIFEEASPPGRRAGAPESAIERLRKTKFDGFNDEEEEEVGDCSVCYEDLKSKGKEVSRIPCGHLYHKSCILTWLERSNSCPLCRASLNP
Homology
[Close]
BLAST of Lsi07G009140 vs. Swiss-Prot
Match: RING1_GOSHI (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum GN=RING1 PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 4.1e-10
Identity = 33/73 (45.21%), Postives = 42/73 (57.53%), Query Frame = 1 

Query: 161 APESAIERLRKTKFDGFNDEEEEEVGDCSVCYEDLKSKGKEVSRIPCGHLYHKSCILTWL 220
           A +SAIE L        N     E   C+VC +D + +G E  ++PC HLYHK C+L WL
Sbjct: 199 ASKSAIEALPLVNITKSN--LNSEFNQCAVCMDDFE-EGTEAKQMPCKHLYHKDCLLPWL 258

Query: 221 ERSNSCPLCRASL 234
           E  NSCP+CR  L
Sbjct: 259 ELHNSCPVCRHEL 268

[Close]
BLAST of Lsi07G009140 vs. Swiss-Prot
Match: RN126_MOUSE (E3 ubiquitin-protein ligase RNF126 OS=Mus musculus GN=Rnf126 PE=1 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 2.0e-09
Identity = 29/55 (52.73%), Postives = 39/55 (70.91%), Query Frame = 1 

Query: 182 EEEVG---DCSVCYEDLKSKGKEVSRIPCGHLYHKSCILTWLERSNSCPLCRASL 234
           EE VG   +C VC ED  + G+ V ++PC HL+H SCI+ WLE+ +SCP+CR SL
Sbjct: 222 EEHVGSGLECPVCKEDY-ALGESVRQLPCNHLFHDSCIVPWLEQHDSCPVCRKSL 275

[Close]
BLAST of Lsi07G009140 vs. Swiss-Prot
Match: AMFR_HUMAN (E3 ubiquitin-protein ligase AMFR OS=Homo sapiens GN=AMFR PE=1 SV=2)

HSP 1 Score: 63.5 bits (153), Expect = 3.4e-09
Identity = 23/48 (47.92%), Postives = 35/48 (72.92%), Query Frame = 1 

Query: 187 DCSVCYEDLKSKGKEVSRIPCGHLYHKSCILTWLERSNSCPLCRASLN 235
           DC++C++ +++  K    +PCGHL+H SC+ +WLE+  SCP CR SLN
Sbjct: 340 DCAICWDSMQAARK----LPCGHLFHNSCLRSWLEQDTSCPTCRMSLN 383

[Close]
BLAST of Lsi07G009140 vs. Swiss-Prot
Match: AMFR_MOUSE (E3 ubiquitin-protein ligase AMFR OS=Mus musculus GN=Amfr PE=1 SV=2)

HSP 1 Score: 63.5 bits (153), Expect = 3.4e-09
Identity = 23/48 (47.92%), Postives = 35/48 (72.92%), Query Frame = 1 

Query: 187 DCSVCYEDLKSKGKEVSRIPCGHLYHKSCILTWLERSNSCPLCRASLN 235
           DC++C++ +++  K    +PCGHL+H SC+ +WLE+  SCP CR SLN
Sbjct: 340 DCAICWDSMQAARK----LPCGHLFHNSCLRSWLEQDTSCPTCRMSLN 383

[Close]
BLAST of Lsi07G009140 vs. Swiss-Prot
Match: RN126_XENTR (E3 ubiquitin-protein ligase RNF126 OS=Xenopus tropicalis GN=rnf126 PE=2 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 4.5e-09
Identity = 27/56 (48.21%), Postives = 39/56 (69.64%), Query Frame = 1 

Query: 182 EEEVG---DCSVCYEDLKSKGKEVSRIPCGHLYHKSCILTWLERSNSCPLCRASLN 235
           EE VG   +C VC ED  + G+ V ++PC HL+H  CI+ WLE+ ++CP+CR SL+
Sbjct: 218 EEHVGSGLECPVCKEDY-TVGESVRQLPCNHLFHNDCIIPWLEQHDTCPVCRKSLS 272

[Close]
BLAST of Lsi07G009140 vs. TrEMBL
Match: A0A0A0LHS2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G130680 PE=4 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 1.4e-78
Identity = 149/235 (63.40%), Postives = 175/235 (74.47%), Query Frame = 1 

Query: 1   MSFSTTSSIQYCFRGIPNNEQILEIRFGYCSRLISRSPSPEIIPDLILQQTPSTLIGETL 60
           MSFS TS +Q  F G PN +Q L +R  +CSRLIS+S +PEI+PD+IL+QTP TLIGE L
Sbjct: 1   MSFSATSCVQCSFSGYPNTDQTLIVRLRFCSRLISQSLAPEIVPDVILEQTPFTLIGEAL 60

Query: 61  FSFSNLQDRQQQQAIKFVVNGFLLSFNLHVSVCQIISRKILSFGQQFDTNNGSFKIEANI 120
           FS SNLQ   QQQ ++++VN FL SFNL  SVC+I+S  ILSF QQFD NN  FKIE N+
Sbjct: 61  FSLSNLQYPLQQQELEYMVNEFLRSFNLRSSVCRIVSHNILSFAQQFDINNECFKIETNV 120

Query: 121 DYMKEFWMEAVPGPYPISIIGEEEFGSIFEEASPPGRRAGAPESAIERLRKTKFDGFNDE 180
           DY+KE+W EA P  YPIS +G        EE  PP    GA ESAIER++K KFDGF  E
Sbjct: 121 DYIKEYWTEAAPELYPISTVG--------EEGLPP---RGASESAIERVKKQKFDGFRGE 180

Query: 181 EEEEVGDCSVCYEDLKSKGKEVSRIPCGHLYHKSCILTWLERSNSCPLCRASLNP 236
           EEEE+GDCSVC E++K KG EV RIPCGH+YHKSCIL WLE SNSCPLCRA+L P
Sbjct: 181 EEEEIGDCSVCCEEIKRKGGEVRRIPCGHVYHKSCILKWLEISNSCPLCRAALEP 224

[Close]
BLAST of Lsi07G009140 vs. TrEMBL
Match: A0A0A0LRB0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G432790 PE=4 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 1.4e-30
Identity = 98/246 (39.84%), Postives = 125/246 (50.81%), Query Frame = 1 

Query: 1   MSFSTTSSIQYCFRGIPNNE--------QILEIRFGYCSRLISRSPSPEIIPDLILQQTP 60
           MS  TT S +   R   N          Q +E+R G+ S+L+  SPS +I     LQQT 
Sbjct: 1   MSSPTTFSFRCSIRCNANGSAATNGQPLQFVELRLGHFSQLVL-SPSHQI-----LQQTS 60

Query: 61  STLIGETLFSFSNLQDRQQQQAIKFVVNGFLLSFNLHVSVCQIISRKILSFGQQFDTNN- 120
             LI +TL  F   +           +  FL S+N+  +VC  I  KI SF  Q    N 
Sbjct: 61  PILISDTLIRFPLRELEDPPFISHSSLCRFLSSYNISDAVCDAIFLKISSFAFQLVAGNL 120

Query: 121 -GSFKIEANIDYMKEFWMEAVPGPYPISIIGEEEFGSIFEEASPPGRRAGAPESAIERLR 180
             +F I A +D++   W++  P     ++                  R GAP SAIERL 
Sbjct: 121 NSNFHIIAALDFVYTHWVDLDPAEDAAAV------------------RQGAPRSAIERLM 180

Query: 181 KTKFDGFNDEEEEEVGDCSVCYEDLKSKG---KEVSRIPCGHLYHKSCILTWLERSNSCP 234
           K K+DG   EE E+  +CSVCYEDL  K    KEVSRIPCGH++HKSCIL WL+ SNSCP
Sbjct: 181 KEKYDGNGGEEMED--ECSVCYEDLHGKTEKEKEVSRIPCGHMFHKSCILKWLKISNSCP 220

[Close]
BLAST of Lsi07G009140 vs. TrEMBL
Match: A0A0A0L3Z2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G551140 PE=4 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.0e-28
Identity = 90/225 (40.00%), Postives = 118/225 (52.44%), Query Frame = 1 

Query: 21  QILEIRFGYCSRLISRSPSPEIIPDLILQQTPSTLIGETLFSFSNLQDRQQQQAIKFVVN 80
           Q +++R  +  +L+  +PS +I     LQQT  TLI +TL  FS  +         F + 
Sbjct: 29  QFVQLRLRHFGQLV-HTPSQQI-----LQQTSPTLISDTLIPFSLWELEDPPFISYFFLF 88

Query: 81  GFLLSFNLHVSVCQIISRKILSFGQQF----DTN-NGSFKIEANIDYMKEFWMEAVPGPY 140
            FL S+++  +VC  I   I SF  Q     D+N N +F I A++D +   W++  P   
Sbjct: 89  RFLSSYDISAAVCDAIFYNISSFAGQILLAADSNINVNFHIIADVDIVYTHWVDLDPA-- 148

Query: 141 PISIIGEEEFGSIFEEASPPGRRAGAPESAIERLRKTKFDGFNDEEEEEVGDCSVCYEDL 200
                         E+A  P  R GA  S IER+ K K+DG   EE+E    CSVCYE L
Sbjct: 149 --------------EDA--PAVRKGASTSVIERVMKEKYDGNGGEEKEGDDQCSVCYEQL 208

Query: 201 -------KSKGKEVSRIPCGHLYHKSCILTWLERSNSCPLCRASL 234
                    K KEVSRIPCGH+YHKSCIL WL+ SNSCPLCR  L
Sbjct: 209 YGKRENENEKEKEVSRIPCGHMYHKSCILKWLKISNSCPLCRREL 229

[Close]
BLAST of Lsi07G009140 vs. TrEMBL
Match: A0A0A0K696_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G026750 PE=4 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 2.2e-26
Identity = 80/237 (33.76%), Postives = 125/237 (52.74%), Query Frame = 1 

Query: 1   MSFSTTSSIQY-CFRGIPNNEQILEIRFGYCSRLISRSPSPEIIPDLILQQTPSTLIGET 60
           MSF+ ++ IQY  +        +L++   +  ++IS SP     P  +L QTP       
Sbjct: 1   MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGR-PHEVLHQTPRIPYHNA 60

Query: 61  LFSFSNLQDRQQQQAIKFVVNGFLLSFNLHVSVCQIISRKILSFGQQFDTNNG--SFKIE 120
           LF  S     +Q Q+  F +   L S N+  +  Q ++R+I S   Q    N   +F I 
Sbjct: 61  LFYLS----LRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHIL 120

Query: 121 ANIDYMKEFWMEAVPGPYPISIIGEEEFGSIFEEASPPGRRAGAPESAIERLRKTKFDGF 180
           A +D+++  W+ ++ G       G EE  ++  + +PP  + G   +  ERLR       
Sbjct: 121 AEVDFIRLIWLPSMGG----GGAGAEE--TVVLDEAPPAVKRGVGVARGERLR------- 180

Query: 181 NDEEEEEVGDCSVCYEDLKSKGKEVSRIPCGHLYHKSCILTWLERSNSCPLCRASLN 235
           ++E+ EE+GDCS+C ++L  + +EV RIPCGH+YH+SCI  WLE  NSCPLCR  L+
Sbjct: 181 SEEKMEELGDCSICLDELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLH 219

[Close]
BLAST of Lsi07G009140 vs. TrEMBL
Match: A0A0A0K2W2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G025210 PE=4 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 2.2e-26
Identity = 80/237 (33.76%), Postives = 125/237 (52.74%), Query Frame = 1 

Query: 1   MSFSTTSSIQY-CFRGIPNNEQILEIRFGYCSRLISRSPSPEIIPDLILQQTPSTLIGET 60
           MSF+ ++ IQY  +        +L++   +  ++IS SP     P  +L QTP       
Sbjct: 1   MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGR-PHEVLHQTPRIPYHNA 60

Query: 61  LFSFSNLQDRQQQQAIKFVVNGFLLSFNLHVSVCQIISRKILSFGQQFDTNNG--SFKIE 120
           LF  S     +Q Q+  F +   L S N+  +  Q ++R+I S   Q    N   +F I 
Sbjct: 61  LFYLS----LRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHIL 120

Query: 121 ANIDYMKEFWMEAVPGPYPISIIGEEEFGSIFEEASPPGRRAGAPESAIERLRKTKFDGF 180
           A +D+++  W+ ++ G       G EE  ++  + +PP  + G   +  ERLR       
Sbjct: 121 AEVDFIRLIWLPSMGG----GGAGAEE--TVVLDEAPPAVKRGVGVARGERLR------- 180

Query: 181 NDEEEEEVGDCSVCYEDLKSKGKEVSRIPCGHLYHKSCILTWLERSNSCPLCRASLN 235
           ++E+ EE+GDCS+C ++L  + +EV RIPCGH+YH+SCI  WLE  NSCPLCR  L+
Sbjct: 181 SEEKMEELGDCSICLDELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLH 219

[Close]
BLAST of Lsi07G009140 vs. TAIR10
Match: AT3G19950.1 (AT3G19950.1 RING/U-box superfamily protein)

HSP 1 Score: 63.2 bits (152), Expect = 2.5e-10
Identity = 29/73 (39.73%), Postives = 45/73 (61.64%), Query Frame = 1 

Query: 161 APESAIERLRKTKFDGFNDEEEEEVGDCSVCYEDLKSKGKEVSRIPCGHLYHKSCILTWL 220
           A +SAI+ L   K     D  + E+  C+VC ++ +  G +V ++PC H++H+ C+L WL
Sbjct: 191 ASKSAIDALPTVKVT--KDMLKSEMNQCAVCMDEFED-GSDVKQMPCKHVFHQDCLLPWL 250

Query: 221 ERSNSCPLCRASL 234
           E  NSCP+CR  L
Sbjct: 251 ELHNSCPVCRFEL 260

[Close]
BLAST of Lsi07G009140 vs. TAIR10
Match: AT4G12190.1 (AT4G12190.1 RING/U-box superfamily protein)

HSP 1 Score: 62.8 bits (151), Expect = 3.3e-10
Identity = 24/52 (46.15%), Postives = 35/52 (67.31%), Query Frame = 1 

Query: 184 EVGDCSVCYEDLKS--KGKEVSRIPCGHLYHKSCILTWLERSNSCPLCRASL 234
           E   CS+C E L S  K ++++R+ C H++H  C+L WL+R N+CPLCR  L
Sbjct: 18  ETEPCSICLESLVSGPKPRDITRMTCSHVFHNGCLLEWLKRKNTCPLCRTEL 69

[Close]
BLAST of Lsi07G009140 vs. TAIR10
Match: AT4G05350.1 (AT4G05350.1 RING/U-box superfamily protein)

HSP 1 Score: 62.0 bits (149), Expect = 5.6e-10
Identity = 27/68 (39.71%), Postives = 41/68 (60.29%), Query Frame = 1 

Query: 168 RLRKTKFDGFNDEEEEEVGDCSVCYEDLKS--KGKEVSRIPCGHLYHKSCILTWLERSNS 227
           R++  +F     E E     CS+C E L S  K ++V+R+ C H++H  C+L WL+R N+
Sbjct: 141 RMKAEEFKSLKMETEP----CSICLESLVSGPKPRDVTRMTCSHVFHNGCLLEWLKRKNT 200

Query: 228 CPLCRASL 234
           CPLCR  +
Sbjct: 201 CPLCRTEI 204

[Close]
BLAST of Lsi07G009140 vs. TAIR10
Match: AT2G44330.1 (AT2G44330.1 RING/U-box superfamily protein)

HSP 1 Score: 61.6 bits (148), Expect = 7.4e-10
Identity = 24/46 (52.17%), Postives = 29/46 (63.04%), Query Frame = 1 

Query: 188 CSVCYEDLKSKGKEVSRIPCGHLYHKSCILTWLERSNSCPLCRASL 234
           C++C ED    G+   R+PC HLYH  CI+ WL   NSCPLCR  L
Sbjct: 96  CAICREDFVV-GESARRLPCNHLYHNDCIIPWLTSHNSCPLCRVEL 140

[Close]
BLAST of Lsi07G009140 vs. TAIR10
Match: AT2G40830.2 (AT2G40830.2 RING-H2 finger C1A)

HSP 1 Score: 61.6 bits (148), Expect = 7.4e-10
Identity = 33/89 (37.08%), Postives = 48/89 (53.93%), Query Frame = 1 

Query: 148 IFEEASPPGRRAG---APESAIERLRKTKFDGFNDEEEEEVGDCSVCYEDLKSKGKEVSR 207
           +FE+ S    R G   AP SAI+ L   K    +    +   +C VC ++ +  G E  +
Sbjct: 149 LFEQLSAGTTRRGPPPAPRSAIDALPTIKIAQRHLRSSDS--NCPVCKDEFEL-GSEAKQ 208

Query: 208 IPCGHLYHKSCILTWLERSNSCPLCRASL 234
           +PC H+YH  CI+ WL + NSCP+CR  L
Sbjct: 209 MPCNHIYHSDCIVPWLVQHNSCPVCRQEL 234

[Close]
BLAST of Lsi07G009140 vs. NCBI nr
Match: gi|449442467|ref|XP_004139003.1| (PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis sativus])

HSP 1 Score: 300.8 bits (769), Expect = 2.0e-78
Identity = 149/235 (63.40%), Postives = 175/235 (74.47%), Query Frame = 1 

Query: 1   MSFSTTSSIQYCFRGIPNNEQILEIRFGYCSRLISRSPSPEIIPDLILQQTPSTLIGETL 60
           MSFS TS +Q  F G PN +Q L +R  +CSRLIS+S +PEI+PD+IL+QTP TLIGE L
Sbjct: 1   MSFSATSCVQCSFSGYPNTDQTLIVRLRFCSRLISQSLAPEIVPDVILEQTPFTLIGEAL 60

Query: 61  FSFSNLQDRQQQQAIKFVVNGFLLSFNLHVSVCQIISRKILSFGQQFDTNNGSFKIEANI 120
           FS SNLQ   QQQ ++++VN FL SFNL  SVC+I+S  ILSF QQFD NN  FKIE N+
Sbjct: 61  FSLSNLQYPLQQQELEYMVNEFLRSFNLRSSVCRIVSHNILSFAQQFDINNECFKIETNV 120

Query: 121 DYMKEFWMEAVPGPYPISIIGEEEFGSIFEEASPPGRRAGAPESAIERLRKTKFDGFNDE 180
           DY+KE+W EA P  YPIS +G        EE  PP    GA ESAIER++K KFDGF  E
Sbjct: 121 DYIKEYWTEAAPELYPISTVG--------EEGLPP---RGASESAIERVKKQKFDGFRGE 180

Query: 181 EEEEVGDCSVCYEDLKSKGKEVSRIPCGHLYHKSCILTWLERSNSCPLCRASLNP 236
           EEEE+GDCSVC E++K KG EV RIPCGH+YHKSCIL WLE SNSCPLCRA+L P
Sbjct: 181 EEEEIGDCSVCCEEIKRKGGEVRRIPCGHVYHKSCILKWLEISNSCPLCRAALEP 224

[Close]
BLAST of Lsi07G009140 vs. NCBI nr
Match: gi|659114890|ref|XP_008457277.1| (PREDICTED: RING finger protein 165-like [Cucumis melo])

HSP 1 Score: 293.1 bits (749), Expect = 4.3e-76
Identity = 148/235 (62.98%), Postives = 176/235 (74.89%), Query Frame = 1 

Query: 1   MSFSTTSSIQYCFRGIPNNEQILEIRFGYCSRLISRSPSPEIIPDLILQQTPSTLIGETL 60
           MSFS TS ++  F G PN +Q L +RFG CSRLIS+SPSPEIIPD+IL+QTP TLIGE L
Sbjct: 1   MSFSATSCLECSFSGYPNTDQTLIVRFGSCSRLISQSPSPEIIPDVILEQTPFTLIGEAL 60

Query: 61  FSFSNLQDRQQQQAIKFVVNGFLLSFNLHVSVCQIISRKILSFGQQFDTNNGSFKIEANI 120
           FS SNL+   QQQ ++++VN FL SFNL  SVC+IIS  ILSF QQFD NN   KIEAN+
Sbjct: 61  FSLSNLRCPLQQQELQYLVNEFLHSFNLRSSVCRIISHNILSFAQQFDINNEWVKIEANV 120

Query: 121 DYMKEFWMEAVPGPYPISIIGEEEFGSIFEEASPPGRRAGAPESAIERLRKTKFDGFNDE 180
           D++KE+W EA P   PIS +GEE         SP     GA ESAIER++K K D F++E
Sbjct: 121 DHIKEYWTEAPPELNPISTVGEEGL-------SP----RGASESAIERVKKQKLDWFSEE 180

Query: 181 EEEEVGDCSVCYEDLKSKGKEVSRIPCGHLYHKSCILTWLERSNSCPLCRASLNP 236
           EEEE+GDC VC E++K KG+EV RIPCGH+YHKSCIL WLE SNSCPLCRA+L P
Sbjct: 181 EEEEIGDCCVCCEEMKRKGEEVRRIPCGHVYHKSCILKWLEISNSCPLCRAALEP 224

[Close]
BLAST of Lsi07G009140 vs. NCBI nr
Match: gi|659121671|ref|XP_008460768.1| (PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo])

HSP 1 Score: 246.1 bits (627), Expect = 6.0e-62
Identity = 137/237 (57.81%), Postives = 161/237 (67.93%), Query Frame = 1 

Query: 1   MSFSTTSSIQYCFRGIPNNEQILEIRFGYCSRLISRSPSPEIIPDLILQQTPSTLIGETL 60
           MS ST+SS Q  F   PN +  L  RFGY SRLI        I D ILQQTPS L+GETL
Sbjct: 1   MSLSTSSSFQCSFSATPNTDHFLICRFGYSSRLI--------IVDQILQQTPSILMGETL 60

Query: 61  FSFSNLQDRQQQQAIKFVVNGFLLSFNLH--VSVCQIISRKILSFGQQFDTNNGSFKIEA 120
             FSNL   +QQQ+I+F+VN FL SFNLH   S  + I RKILSF  QF   +GSFKIEA
Sbjct: 61  IPFSNLWHPEQQQSIEFLVNEFLGSFNLHSFASARRTICRKILSFALQFHNVHGSFKIEA 120

Query: 121 NIDYMKEFWMEAVPGPYPISIIGEEEFGSIFEEASPPGRRAGAPESAIERLRKTKFDGFN 180
           N++++++             IIGE+ F    +E   P R  GAPESAIERLRK KFD  N
Sbjct: 121 NVEHVEDL------------IIGEQGF----QETPSPPRTTGAPESAIERLRKEKFDVLN 180

Query: 181 DEEEEEVGDCSVCYEDLKSKGKEVSRIPCGHLYHKSCILTWLERSNSCPLCRASLNP 236
             +EEE+GDCSVCYE+LK +GKEVSRIPCGH+YHKSCILTWL+ +NSCPLCRA L P
Sbjct: 181 --KEEEIGDCSVCYEELKGQGKEVSRIPCGHVYHKSCILTWLQINNSCPLCRAPLQP 211

[Close]
BLAST of Lsi07G009140 vs. NCBI nr
Match: gi|659083325|ref|XP_008442291.1| (PREDICTED: RING finger protein 165-like [Cucumis melo])

HSP 1 Score: 208.0 bits (528), Expect = 1.8e-50
Identity = 109/179 (60.89%), Postives = 131/179 (73.18%), Query Frame = 1 

Query: 56  IGETLFSFSNLQDRQQQQAIKFVVNGFLLSFNLHVSVCQIISRKILSFGQQFDTNNGSFK 115
           +G+TL  FSNL+  +QQQ+I+F+VN FL SFNLH SV + I  +ILSF  QF     SFK
Sbjct: 1   MGDTLIPFSNLRHPRQQQSIQFLVNQFLCSFNLHSSVRRTICHEILSFALQFRAILPSFK 60

Query: 116 IEANIDYMKEFWMEAVPGPYPISIIGEEEFGSIFEEASPPGRRAGAPESAIERL-RKTKF 175
           IEANID++++             IIGE+ F     + +PP R  GAPESAIERL  K KF
Sbjct: 61  IEANIDHVEDL------------IIGEQGF-----QETPPPRTRGAPESAIERLIMKQKF 120

Query: 176 DGFNDEEEEEVGDCSVCYEDLKSKGKEVSRIPCGHLYHKSCILTWLERSNSCPLCRASL 234
           DGFN EEEEE+GDCSVCYE+LK KGKEVSRIPCGH+YHKSC+LTWL+ +NSCPLCRA L
Sbjct: 121 DGFNKEEEEEIGDCSVCYEELKGKGKEVSRIPCGHVYHKSCVLTWLQINNSCPLCRAPL 162

[Close]
BLAST of Lsi07G009140 vs. NCBI nr
Match: gi|778674460|ref|XP_011650219.1| (PREDICTED: RING-H2 finger protein ATL16-like [Cucumis sativus])

HSP 1 Score: 141.4 bits (355), Expect = 2.1e-30
Identity = 98/246 (39.84%), Postives = 125/246 (50.81%), Query Frame = 1 

Query: 1   MSFSTTSSIQYCFRGIPNNE--------QILEIRFGYCSRLISRSPSPEIIPDLILQQTP 60
           MS  TT S +   R   N          Q +E+R G+ S+L+  SPS +I     LQQT 
Sbjct: 1   MSSPTTFSFRCSIRCNANGSAATNGQPLQFVELRLGHFSQLVL-SPSHQI-----LQQTS 60

Query: 61  STLIGETLFSFSNLQDRQQQQAIKFVVNGFLLSFNLHVSVCQIISRKILSFGQQFDTNN- 120
             LI +TL  F   +           +  FL S+N+  +VC  I  KI SF  Q    N 
Sbjct: 61  PILISDTLIRFPLRELEDPPFISHSSLCRFLSSYNISDAVCDAIFLKISSFAFQLVAGNL 120

Query: 121 -GSFKIEANIDYMKEFWMEAVPGPYPISIIGEEEFGSIFEEASPPGRRAGAPESAIERLR 180
             +F I A +D++   W++  P     ++                  R GAP SAIERL 
Sbjct: 121 NSNFHIIAALDFVYTHWVDLDPAEDAAAV------------------RQGAPRSAIERLM 180

Query: 181 KTKFDGFNDEEEEEVGDCSVCYEDLKSKG---KEVSRIPCGHLYHKSCILTWLERSNSCP 234
           K K+DG   EE E+  +CSVCYEDL  K    KEVSRIPCGH++HKSCIL WL+ SNSCP
Sbjct: 181 KEKYDGNGGEEMED--ECSVCYEDLHGKTEKEKEVSRIPCGHMFHKSCILKWLKISNSCP 220

The following BLAST results are available for this feature:
BLAST of Lsi07G009140 vs. Swiss-Prot
Match NameE-valueIdentityDescription
RING1_GOSHI4.1e-1045.21E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum GN=RING1 PE=1 SV=1[more]
RN126_MOUSE2.0e-0952.73E3 ubiquitin-protein ligase RNF126 OS=Mus musculus GN=Rnf126 PE=1 SV=1[more]
AMFR_HUMAN3.4e-0947.92E3 ubiquitin-protein ligase AMFR OS=Homo sapiens GN=AMFR PE=1 SV=2[more]
AMFR_MOUSE3.4e-0947.92E3 ubiquitin-protein ligase AMFR OS=Mus musculus GN=Amfr PE=1 SV=2[more]
RN126_XENTR4.5e-0948.21E3 ubiquitin-protein ligase RNF126 OS=Xenopus tropicalis GN=rnf126 PE=2 SV=1[more]
BLAST of Lsi07G009140 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LHS2_CUCSA1.4e-7863.40Uncharacterized protein OS=Cucumis sativus GN=Csa_2G130680 PE=4 SV=1[more]
A0A0A0LRB0_CUCSA1.4e-3039.84Uncharacterized protein OS=Cucumis sativus GN=Csa_2G432790 PE=4 SV=1[more]
A0A0A0L3Z2_CUCSA1.0e-2840.00Uncharacterized protein OS=Cucumis sativus GN=Csa_4G551140 PE=4 SV=1[more]
A0A0A0K696_CUCSA2.2e-2633.76Uncharacterized protein OS=Cucumis sativus GN=Csa_7G026750 PE=4 SV=1[more]
A0A0A0K2W2_CUCSA2.2e-2633.76Uncharacterized protein OS=Cucumis sativus GN=Csa_7G025210 PE=4 SV=1[more]
BLAST of Lsi07G009140 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G19950.12.5e-1039.73 RING/U-box superfamily protein[more]
AT4G12190.13.3e-1046.15 RING/U-box superfamily protein[more]
AT4G05350.15.6e-1039.71 RING/U-box superfamily protein[more]
AT2G44330.17.4e-1052.17 RING/U-box superfamily protein[more]
AT2G40830.27.4e-1037.08 RING-H2 finger C1A[more]
BLAST of Lsi07G009140 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449442467|ref|XP_004139003.1|2.0e-7863.40PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis sativus][more]
gi|659114890|ref|XP_008457277.1|4.3e-7662.98PREDICTED: RING finger protein 165-like [Cucumis melo][more]
gi|659121671|ref|XP_008460768.1|6.0e-6257.81PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo][more]
gi|659083325|ref|XP_008442291.1|1.8e-5060.89PREDICTED: RING finger protein 165-like [Cucumis melo][more]
gi|778674460|ref|XP_011650219.1|2.1e-3039.84PREDICTED: RING-H2 finger protein ATL16-like [Cucumis sativus][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0008270zinc ion binding
GO:0005515protein binding
Vocabulary: INTERPRO
TermDefinition
IPR013083Znf_RING/FYVE/PHD
IPR001841Znf_RING
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005515 protein binding
molecular_function GO:0008270 zinc ion binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi07G009140.1Lsi07G009140.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 187..230
score: 1.1
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 188..229
score: 1.
IPR001841Zinc finger, RING-typePROFILEPS50089ZF_RING_2coord: 188..230
score: 13
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 183..231
score: 8.1
NoneNo IPR availablePANTHERPTHR22763RING ZINC FINGER PROTEINcoord: 17..47
score: 8.7E-21coord: 110..233
score: 8.7
NoneNo IPR availableunknownSSF57850RING/U-boxcoord: 179..233
score: 2.43

Synteny
The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Lsi07G009140Cucumber (Gy14) v1cgylsiB141
Lsi07G009140Cucurbita maxima (Rimu)cmalsiB328
Lsi07G009140Cucurbita maxima (Rimu)cmalsiB700
Lsi07G009140Cucurbita moschata (Rifu)cmolsiB317
Lsi07G009140Cucurbita moschata (Rifu)cmolsiB694
Lsi07G009140Cucurbita pepo (Zucchini)cpelsiB187
Lsi07G009140Cucurbita pepo (Zucchini)cpelsiB379
Lsi07G009140Wild cucumber (PI 183967)cpilsiB306
Lsi07G009140Cucumber (Chinese Long) v2culsiB306
Lsi07G009140Melon (DHL92) v3.5.1lsimeB457
Lsi07G009140Watermelon (Charleston Gray)lsiwcgB415
Lsi07G009140Watermelon (97103) v1lsiwmB446
Lsi07G009140Cucumber (Gy14) v2cgyblsiB280
Lsi07G009140Melon (DHL92) v3.6.1lsimedB475
Lsi07G009140Silver-seed gourdcarlsiB535
Lsi07G009140Silver-seed gourdcarlsiB860
Lsi07G009140Cucumber (Chinese Long) v3cuclsiB325
Lsi07G009140Watermelon (97103) v2lsiwmbB414
Lsi07G009140Wax gourdlsiwgoB556