Lsi07G008250 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi07G008250
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Serine/threonine-protein kinase
Location	chr07 : 9191165 .. 9192417 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACTTGAGTTTGGAAGTGAATTCTGGGTCAGAAGTAAGCAGTTTCTCTGGAGGTTACCTTGGCTAGGTGATGCTTTGCTCACCATTATCTTTCATGGCTGCTTCTTGAAGCCTATTGAGCTTTACTTCATACCATTATGATAAACATATCTTATCACTTATGAGGGAACTATCTCAAGTTTTCATTTCATGGAGGAATCTCCAATTCTCTACTTTCCATTTGGGTTCAACGTTAGTATTCCGGTCCTAAAAATTCCACCGAGCCCCATAGATCTAGAAAATCATCTAGCTAATTCCACTTGAAAGGATGCTGCTGGTGAATGATGACAGGGGATGCGGTTTACTTTTTGTTGGGCAGTGGCAAGGAACATATGTTTGTTTGCTATATCTGAGCAAATAATCTTTGCAAACGTCAATTAATAATGAATTGTTGTTCATTTGCACATTCTCTGGGGAGGGGAGGTCTGAGTTCTATTGGGTATGGATCTTGTCATGATGCAAACCTGCTGCATTGTTGATTGTTTCTTGTTTGGCATTTTGTGGGTGTGTCATGTGTGTGTGTTTTAATCAAATAAACTTGCATTTATAAGCTGGAGAACTATACATTTTCCATCATGAGATTATATGAACTGAGAAAATCATTAAGCAAATCATATTATATTTGGACTATAATTGTACAAGAGTTGCCTCCATGGACTGCCTCTGCATTGATAATCTTCTTACTAAAAGTTAACAGCATCTGAGATTCTTTTACATTTATTCTGTTCTATCCATATATCTAATTCTAAACTGATTTGTTTCAGTATTTAAAGCCAGTTTGGTCATAACACTCCTTGAAGGAGCGGAGAACAGCTGCTGCTTTATCATATAGATATGACTTGCTTCCCTTTTTTGTTCAGTACGAGGGTAAAACCCCCAACAAGAAGTTTTGTCGATGAAGAAGGTAAATTAATCACCGGGCATGCATAACATCATCTTTTTATGGACATATTTGAAGAGTTTCTTGTTGTTAAAATTTAAAATATGTAAAATGAATGTGCAACACTGTGCAGTCCTTTCTGCAAGGAAATTCTAACATTCTTATTCTTCTTCTATGGAATAGAATTTTCGAGCATGCAAAATGTTAAATTTTACACATACAAAGAGCTAAAAGTTGCAACTGAAGATTTCAGCTTGGCGAATAAAATAGGGGAGGGAGGTTTTGGTTCTGTCTACCAGGTAGCTGCATATCTCCTTTCACATGAACTATAG

mRNA sequence

ATGAACTTGAGTTTGGAAGTGAATTCTGGTCCTTTCTGCAAGGAAATTCTAACATTCTTATTCTTCTTCTATGGAATAGAATTTTCGAGCATGCAAAATGTTAAATTTTACACATACAAAGAGCTAAAAGTTGCAACTGAAGATTTCAGCTTGGCGAATAAAATAGGGGAGGGAGGTTTTGGTTCTGTCTACCAGGTAGCTGCATATCTCCTTTCACATGAACTATAG

Coding sequence (CDS)

ATGAACTTGAGTTTGGAAGTGAATTCTGGTCCTTTCTGCAAGGAAATTCTAACATTCTTATTCTTCTTCTATGGAATAGAATTTTCGAGCATGCAAAATGTTAAATTTTACACATACAAAGAGCTAAAAGTTGCAACTGAAGATTTCAGCTTGGCGAATAAAATAGGGGAGGGAGGTTTTGGTTCTGTCTACCAGGTAGCTGCATATCTCCTTTCACATGAACTATAG

Protein sequence

MNLSLEVNSGPFCKEILTFLFFFYGIEFSSMQNVKFYTYKELKVATEDFSLANKIGEGGFGSVYQVAAYLLSHEL

Homology

[Close]

BLAST of Lsi07G008250 vs. TrEMBL
Match: A0A140G4I5_9ROSI (LRR-RLK OS=Vernicia montana PE=2 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 1.9e-08
Identity = 31/39 (79.49%), Postives = 34/39 (87.18%), Query Frame = 1


Query: 27 EFSSMQNVKFYTYKELKVATEDFSLANKIGEGGFGSVYQ 66
          E + + NVKFYTYKELK ATEDFS ANKIGEGGFGSVY+
Sbjct: 25 EVAGLHNVKFYTYKELKNATEDFSPANKIGEGGFGSVYK 63

[Close]

BLAST of Lsi07G008250 vs. TrEMBL
Match: A0A061F917_THECC (LRR receptor-like serine/threonine-protein kinase OS=Theobroma cacao GN=TCM_032498 PE=3 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 5.5e-08
Identity = 30/35 (85.71%), Postives = 32/35 (91.43%), Query Frame = 1


Query: 31 MQNVKFYTYKELKVATEDFSLANKIGEGGFGSVYQ 66
          + NVK YTYKELK ATEDFSLANKIGEGGFGSVY+
Sbjct: 28 IHNVKLYTYKELKSATEDFSLANKIGEGGFGSVYK 62

[Close]

BLAST of Lsi07G008250 vs. TrEMBL
Match: A0A072TQR8_MEDTR (Receptor-like kinase OS=Medicago truncatula GN=MTR_8g463990 PE=3 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 9.4e-08
Identity = 28/40 (70.00%), Postives = 34/40 (85.00%), Query Frame = 1


Query: 26 IEFSSMQNVKFYTYKELKVATEDFSLANKIGEGGFGSVYQ 66
          I+ S + NVK YTYKEL++AT  FSLANKIG+GGFGSVY+
Sbjct: 21 IDISELHNVKIYTYKELRIATSGFSLANKIGQGGFGSVYK 60

[Close]

BLAST of Lsi07G008250 vs. TrEMBL
Match: A0A072TQ83_MEDTR (Receptor-like kinase OS=Medicago truncatula GN=MTR_8g463990 PE=4 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 9.4e-08
Identity = 28/40 (70.00%), Postives = 34/40 (85.00%), Query Frame = 1


Query: 26 IEFSSMQNVKFYTYKELKVATEDFSLANKIGEGGFGSVYQ 66
          I+ S + NVK YTYKEL++AT  FSLANKIG+GGFGSVY+
Sbjct: 21 IDISELHNVKIYTYKELRIATSGFSLANKIGQGGFGSVYK 60

[Close]

BLAST of Lsi07G008250 vs. TrEMBL
Match: A0A067EE14_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g016347mg PE=3 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 1.6e-07
Identity = 28/39 (71.79%), Postives = 33/39 (84.62%), Query Frame = 1


Query: 27 EFSSMQNVKFYTYKELKVATEDFSLANKIGEGGFGSVYQ 66
          EFS + NVK Y+YKEL +AT+DFS  NKIGEGGFGSVY+
Sbjct: 24 EFSGIHNVKLYSYKELSIATDDFSPINKIGEGGFGSVYK 62

[Close]

BLAST of Lsi07G008250 vs. TAIR10
Match: AT1G16670.1 (AT1G16670.1 Protein kinase superfamily protein)

HSP 1 Score: 57.0 bits (136), Expect = 5.8e-09
Identity = 24/39 (61.54%), Postives = 32/39 (82.05%), Query Frame = 1


Query: 27 EFSSMQNVKFYTYKELKVATEDFSLANKIGEGGFGSVYQ 66
          E +++ NVK Y Y+E++ AT+DFS  NKIGEGGFGSVY+
Sbjct: 19 EIAAIDNVKIYKYREIRQATDDFSAENKIGEGGFGSVYK 57

[Close]

BLAST of Lsi07G008250 vs. TAIR10
Match: AT1G56120.1 (AT1G56120.1 Leucine-rich repeat transmembrane protein kinase)

HSP 1 Score: 48.9 bits (115), Expect = 1.6e-06
Identity = 20/29 (68.97%), Postives = 26/29 (89.66%), Query Frame = 1


Query: 37  YTYKELKVATEDFSLANKIGEGGFGSVYQ 66
           +TY ELK AT+DF L+NK+GEGGFG+VY+
Sbjct: 698 FTYSELKNATQDFDLSNKLGEGGFGAVYK 726

[Close]

BLAST of Lsi07G008250 vs. TAIR10
Match: AT4G21230.1 (AT4G21230.1 cysteine-rich RLK (RECEPTOR-like protein kinase) 27)

HSP 1 Score: 47.4 bits (111), Expect = 4.6e-06
Identity = 22/39 (56.41%), Postives = 30/39 (76.92%), Query Frame = 1


Query: 27  EFSSMQNVKFYTYKELKVATEDFSLANKIGEGGFGSVYQ 66
           EF S  ++ F  ++ ++VAT+DFSL NKIGEGGFG VY+
Sbjct: 312 EFESTDSLHF-DFETIRVATDDFSLTNKIGEGGFGVVYK 349

[Close]

BLAST of Lsi07G008250 vs. NCBI nr
Match: gi|449460042|ref|XP_004147755.1| (PREDICTED: putative serine/threonine-protein kinase [Cucumis sativus])

HSP 1 Score: 79.0 bits (193), Expect = 4.0e-12
Identity = 37/39 (94.87%), Postives = 39/39 (100.00%), Query Frame = 1


Query: 27 EFSSMQNVKFYTYKELKVATEDFSLANKIGEGGFGSVYQ 66
          +FSSMQNVKFYTYKELKVATEDFSLANKIGEGGFGSVY+
Sbjct: 24 DFSSMQNVKFYTYKELKVATEDFSLANKIGEGGFGSVYK 62

[Close]

BLAST of Lsi07G008250 vs. NCBI nr
Match: gi|659101847|ref|XP_008451823.1| (PREDICTED: putative serine/threonine-protein kinase [Cucumis melo])

HSP 1 Score: 79.0 bits (193), Expect = 4.0e-12
Identity = 37/39 (94.87%), Postives = 39/39 (100.00%), Query Frame = 1


Query: 27 EFSSMQNVKFYTYKELKVATEDFSLANKIGEGGFGSVYQ 66
          +FSSMQNVKFYTYKELKVATEDFSLANKIGEGGFGSVY+
Sbjct: 24 DFSSMQNVKFYTYKELKVATEDFSLANKIGEGGFGSVYK 62

[Close]

BLAST of Lsi07G008250 vs. NCBI nr
Match: gi|1001910005|gb|AMM42957.1| (LRR-RLK [Vernicia montana])

HSP 1 Score: 66.2 bits (160), Expect = 2.7e-08
Identity = 31/39 (79.49%), Postives = 34/39 (87.18%), Query Frame = 1


Query: 27 EFSSMQNVKFYTYKELKVATEDFSLANKIGEGGFGSVYQ 66
          E + + NVKFYTYKELK ATEDFS ANKIGEGGFGSVY+
Sbjct: 25 EVAGLHNVKFYTYKELKNATEDFSPANKIGEGGFGSVYK 63

[Close]

BLAST of Lsi07G008250 vs. NCBI nr
Match: gi|470116056|ref|XP_004294202.1| (PREDICTED: putative serine/threonine-protein kinase [Fragaria vesca subsp. vesca])

HSP 1 Score: 65.9 bits (159), Expect = 3.5e-08
Identity = 30/39 (76.92%), Postives = 34/39 (87.18%), Query Frame = 1


Query: 27 EFSSMQNVKFYTYKELKVATEDFSLANKIGEGGFGSVYQ 66
          E S + +VK YTYKELK+ATEDF LANKIGEGGFGSVY+
Sbjct: 24 ELSGIHDVKLYTYKELKMATEDFDLANKIGEGGFGSVYK 62

[Close]

BLAST of Lsi07G008250 vs. NCBI nr
Match: gi|645239761|ref|XP_008226283.1| (PREDICTED: putative serine/threonine-protein kinase [Prunus mume])

HSP 1 Score: 64.7 bits (156), Expect = 7.9e-08
Identity = 29/39 (74.36%), Postives = 34/39 (87.18%), Query Frame = 1


Query: 27 EFSSMQNVKFYTYKELKVATEDFSLANKIGEGGFGSVYQ 66
          E S + +VK YTYKE+K+ATEDFS ANKIGEGGFGSVY+
Sbjct: 24 ELSGISDVKLYTYKEMKIATEDFSPANKIGEGGFGSVYK 62

The following BLAST results are available for this feature:

BLAST of Lsi07G008250 vs. Swiss-Prot


Match Name	E-value	Identity	Description

BLAST of Lsi07G008250 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A140G4I5_9ROSI	1.9e-08	79.49	LRR-RLK OS=Vernicia montana PE=2 SV=1	[more]
A0A061F917_THECC	5.5e-08	85.71	LRR receptor-like serine/threonine-protein kinase OS=Theobroma cacao GN=TCM_0324...	[more]
A0A072TQR8_MEDTR	9.4e-08	70.00	Receptor-like kinase OS=Medicago truncatula GN=MTR_8g463990 PE=3 SV=1	[more]
A0A072TQ83_MEDTR	9.4e-08	70.00	Receptor-like kinase OS=Medicago truncatula GN=MTR_8g463990 PE=4 SV=1	[more]
A0A067EE14_CITSI	1.6e-07	71.79	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g016347mg PE=3 SV=1	[more]

BLAST of Lsi07G008250 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G16670.1	5.8e-09	61.54	Protein kinase superfamily protein	[more]
AT1G56120.1	1.6e-06	68.97	Leucine-rich repeat transmembrane protein kinase	[more]
AT4G21230.1	4.6e-06	56.41	cysteine-rich RLK (RECEPTOR-like protein kinase) 27	[more]

BLAST of Lsi07G008250 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449460042\|ref\|XP_004147755.1\|	4.0e-12	94.87	PREDICTED: putative serine/threonine-protein kinase [Cucumis sativus]	[more]
gi\|659101847\|ref\|XP_008451823.1\|	4.0e-12	94.87	PREDICTED: putative serine/threonine-protein kinase [Cucumis melo]	[more]
gi\|1001910005\|gb\|AMM42957.1\|	2.7e-08	79.49	LRR-RLK [Vernicia montana]	[more]
gi\|470116056\|ref\|XP_004294202.1\|	3.5e-08	76.92	PREDICTED: putative serine/threonine-protein kinase [Fragaria vesca subsp. vesca...	[more]
gi\|645239761\|ref\|XP_008226283.1\|	7.9e-08	74.36	PREDICTED: putative serine/threonine-protein kinase [Prunus mume]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR011009	Kinase-like_dom_sf

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046777	protein autophosphorylation
biological_process	GO:0009069	serine family amino acid metabolic process
biological_process	GO:0006468	protein phosphorylation
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004674	protein serine/threonine kinase activity
molecular_function	GO:0004672	protein kinase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi07G008250.1	Lsi07G008250.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011009	Protein kinase-like domain	unknown	SSF56112	Protein kinase-like (PK-like)	coord: 22..68 score: 1.4
None	No IPR available	GENE3D	G3DSA:3.30.200.20		coord: 29..66 score: 7.
None	No IPR available	PANTHER	PTHR27006	FAMILY NOT NAMED	coord: 27..65 score: 3.9
None	No IPR available	PANTHER	PTHR27006:SF22	SUBFAMILY NOT NAMED	coord: 27..65 score: 3.9

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi07G008250	MELO3C016179.2	Melon (DHL92) v3.6.1	lsimedB472
Lsi07G008250	Cla97C07G137440	Watermelon (97103) v2	lsiwmbB411
Lsi07G008250	Bhi01G002408	Wax gourd	lsiwgoB544
Lsi07G008250	Cp4.1LG01g00960	Cucurbita pepo (Zucchini)	cpelsiB372
Lsi07G008250	CsGy4G007280	Cucumber (Gy14) v2	cgyblsiB278
Lsi07G008250	Carg11391	Silver-seed gourd	carlsiB596
Lsi07G008250	Carg13115	Silver-seed gourd	carlsiB043
Lsi07G008250	CsaV3_4G007620	Cucumber (Chinese Long) v3	cuclsiB323

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi07G008250	Cucumber (Gy14) v1	cgylsiB301
Lsi07G008250	Cucurbita maxima (Rimu)	cmalsiB325
Lsi07G008250	Cucurbita maxima (Rimu)	cmalsiB695
Lsi07G008250	Cucurbita moschata (Rifu)	cmolsiB314
Lsi07G008250	Cucurbita moschata (Rifu)	cmolsiB689
Lsi07G008250	Cucurbita pepo (Zucchini)	cpelsiB184
Lsi07G008250	Wild cucumber (PI 183967)	cpilsiB304
Lsi07G008250	Cucumber (Chinese Long) v2	culsiB304
Lsi07G008250	Melon (DHL92) v3.5.1	lsimeB455
Lsi07G008250	Watermelon (Charleston Gray)	lsiwcgB412
Lsi07G008250	Watermelon (97103) v1	lsiwmB440