Lsi07G003990 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi07G003990
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionIndole-3-acetic acid-amido synthetase GH3.17, putative
Locationchr07 : 4299410 .. 4301813 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTAATCCTTTCCCCTTCTGCCTCCCTGCCATTACAGATACCTGGAAAACAGAATCCCTTCACAAAGAAAAAGAAAAAGAAAAAAAAAAAAATTGCATAGTGAAATTGTCAGTTTATAGTTATAGTTATGAACTGATTGAGAGAGAAAAAGAAAAAAAAAAAAAAGCTACCTTTCTTTTGCTGTTTCATTCATTGCAATGTTTAAAATTTAAAAGTCAAGCACTTTCTCTTCAAATTCAAACCCTTTTTTGTTTCTTCTTCTTCTTCTTTAATTCTTCCTGTTCTGATTTGAAAAGATGTTGCCAAAATTTGATCCCAACAACAATGAATCTGGGTTGAAGCTGCTTGAAGATCTTACACAAAATGCAAAGCAAATTCAAGAACAAGTGATTGAGAAGATTCTTACACAAAACTTCAACACAGAATATCTCAAGACTTTTCTCAATGGCCAATCTCCTGATCTCCAAAGTTTCAAGAACTCTGTTCCTGTGGTCAACTATGAAGACATTAAGCCTTATATTGAGAGAATCGCCAATGGTGAACCTTCTCATATAATTTCATCTCAACCCATCACTGAGCTACTCACAAGGTAAATATTAAATTCATCCATTTTTCAACACTTTCAAAAGAAAATTTTCATACTCTCAACCAAATTTGCAGCTCTGGAACTTCTGGAGGACAGCCAAAAATGATGCCCTCAACTGCTGAGGATTTGGACAGAAAGACATTCTTTTACAATCTCCTTGTACCTGTTTTGAACAAGTATGAATAAAACAGTAAACTTTATCATTATTGTTCTAATTTTTTTCTTTTTTCTTTCTTTCTTTAAACATTATAGAGGAAAATTGTTGAAATTTCAGGTATGTGGATGGTTTGGAAGAAGGAAAAGGGATGTATCTTTTGTTTATAAAGCCAGAAATGAGTACCCCATGTGGGTTAATGGCAAGGCCAGTTTTGACAAGTTATTACAAAAGCAAAAACTTCAGAAATAGGCCTTTTAATAGATACAATGTGTACACAAGCCCAGATGAGACAATTCTTTGTTCTGACAGTAAACAGAGTATGTACTGTCAATTGCTTTGTGGGTTATTACAAAGAGATGAAGTTTTAAGAGTTGGTGCAGTTTTTGCCTCTGCTTTTCTTCGAGCTATCAAATTCTTAGAAGATTATTGGAAAGAATTGAGTGATAATATAAGAAATGGGGAAGTTAGTGAATGGATTACTGACTCAAATTGCAGAGCTTCTGTTTCTTTAATCCTTACAAAACCAAATGCTGCTTTGGCTGATTTGATTGATGGTATGTGTGATGAGAAATCATGGGAAGGAATAATTAAGAAGATTTGGCCTAAAACTAAATACATTGAAGTTATTGTAACTGGGTCTATGGCTCAGTATATTCCTACTCTTGAATTTTACAGTGGTGGGCTTCCTTTAGTTTCTACTATGTATGCTTCTTCTGAGTGTTATTTTGGGATTAATTTCAATCCTTTGAGTAAACCTTGTGATGTTTCTTATACACTTCTTCCAAATATGGCCTTCTTTGAATTCTTACCTGTGGAGAAAAATGATGGAGAATTAAGCCATTGCAATGGAAGCTCTGTTCAATCAATTGATGAATTCAAAACAGTTGATCTTGTCGATGTCAAACTTGGTCAATATTACGAATTGGTGGTCACTACTTTCACAGGTGAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAACCCTTTTTTTGTTTTTTGTTTTTGGATCTGACCCACATTATTTTTGCTCTGTTTTTTTTTTTTTTTTTTCTTTTTGGTGGTGGGTTCAGGGTTGTACAGATACAGAGTTGGTGATATTCTAATGGTAACTGGCTTCCACAACAAAGCACCTCAATTCAAGTTTGTTCATAGAAGGAATGTTGTTTTAAGCATAGATACAGATAAGACGAATGAAGATGATTTGTTAAATGCAATTACAAAAGCGAAATTACTTGTTGAACCACTTGGTGTTTTGTTAACAGAGTATACAAGTTATGCAGATACAGGGTCAATTCCAGGGCATTATGTTCTGTTTTGGGAGCTGAAGAAAAGAGGAGGGAATGAGAATTTAGAAGAACTTGAAGTGGGTTTAATGGAAGAATGTTGTTCAAGTATAGAACAATCATTGGATTCAGTTTATAGAAGATGCAGAAGCAAAGATAAATCAATTGGGCCATTGGAGATTAGAGTTGTGAAACATGGAGCTTTTGATGCACTTATGGATTTTTGTGTTTCACAAGGATCTTCTGTGAATCAATACAAAACACCAAGATGCATTAAATCAGAAGAAGCCATCAAAATTCTGGATTCTAGAGTTGTGGGAAGGTATTTCAGCACAAAAACACCATTTTGGGAGCCTTTCAGAGCTCATTACTCCCATTGA

mRNA sequence

ATTTAATCCTTTCCCCTTCTGCCTCCCTGCCATTACAGATACCTGGAAAACAGAATCCCTTCACAAAGAAAAAGAAAAAGAAAAAAAAAAAAATTGCATAGTGAAATTGTCAGTTTATAGTTATAGTTATGAACTGATTGAGAGAGAAAAAGAAAAAAAAAAAAAAGCTACCTTTCTTTTGCTGTTTCATTCATTGCAATGTTTAAAATTTAAAAGTCAAGCACTTTCTCTTCAAATTCAAACCCTTTTTTGTTTCTTCTTCTTCTTCTTTAATTCTTCCTGTTCTGATTTGAAAAGATGTTGCCAAAATTTGATCCCAACAACAATGAATCTGGGTTGAAGCTGCTTGAAGATCTTACACAAAATGCAAAGCAAATTCAAGAACAAGTGATTGAGAAGATTCTTACACAAAACTTCAACACAGAATATCTCAAGACTTTTCTCAATGGCCAATCTCCTGATCTCCAAAGTTTCAAGAACTCTGTTCCTGTGGTCAACTATGAAGACATTAAGCCTTATATTGAGAGAATCGCCAATGGTGAACCTTCTCATATAATTTCATCTCAACCCATCACTGAGCTACTCACAAGCTCTGGAACTTCTGGAGGACAGCCAAAAATGATGCCCTCAACTGCTGAGGATTTGGACAGAAAGACATTCTTTTACAATCTCCTTGTACCTGTTTTGAACAAGTATGTGGATGGTTTGGAAGAAGGAAAAGGGATGTATCTTTTGTTTATAAAGCCAGAAATGAGTACCCCATGTGGGTTAATGGCAAGGCCAGTTTTGACAAGTTATTACAAAAGCAAAAACTTCAGAAATAGGCCTTTTAATAGATACAATGTGTACACAAGCCCAGATGAGACAATTCTTTGTTCTGACAGTAAACAGAGTATGTACTGTCAATTGCTTTGTGGGTTATTACAAAGAGATGAAGTTTTAAGAGTTGGTGCAGTTTTTGCCTCTGCTTTTCTTCGAGCTATCAAATTCTTAGAAGATTATTGGAAAGAATTGAGTGATAATATAAGAAATGGGGAAGTTAGTGAATGGATTACTGACTCAAATTGCAGAGCTTCTGTTTCTTTAATCCTTACAAAACCAAATGCTGCTTTGGCTGATTTGATTGATGGTATGTGTGATGAGAAATCATGGGAAGGAATAATTAAGAAGATTTGGCCTAAAACTAAATACATTGAAGTTATTGTAACTGGGTCTATGGCTCAGTATATTCCTACTCTTGAATTTTACAGTGGTGGGCTTCCTTTAGTTTCTACTATGTATGCTTCTTCTGAGTGTTATTTTGGGATTAATTTCAATCCTTTGAGTAAACCTTGTGATGTTTCTTATACACTTCTTCCAAATATGGCCTTCTTTGAATTCTTACCTGTGGAGAAAAATGATGGAGAATTAAGCCATTGCAATGGAAGCTCTGTTCAATCAATTGATGAATTCAAAACAGTTGATCTTGTCGATGTCAAACTTGGTCAATATTACGAATTGGTGGTCACTACTTTCACAGGGTTGTACAGATACAGAGTTGGTGATATTCTAATGGTAACTGGCTTCCACAACAAAGCACCTCAATTCAAGTTTGTTCATAGAAGGAATGTTGTTTTAAGCATAGATACAGATAAGACGAATGAAGATGATTTGTTAAATGCAATTACAAAAGCGAAATTACTTGTTGAACCACTTGGTGTTTTGTTAACAGAGTATACAAGTTATGCAGATACAGGGTCAATTCCAGGGCATTATGTTCTGTTTTGGGAGCTGAAGAAAAGAGGAGGGAATGAGAATTTAGAAGAACTTGAAGTGGGTTTAATGGAAGAATGTTGTTCAAGTATAGAACAATCATTGGATTCAGTTTATAGAAGATGCAGAAGCAAAGATAAATCAATTGGGCCATTGGAGATTAGAGTTGTGAAACATGGAGCTTTTGATGCACTTATGGATTTTTGTGTTTCACAAGGATCTTCTGTGAATCAATACAAAACACCAAGATGCATTAAATCAGAAGAAGCCATCAAAATTCTGGATTCTAGAGTTGTGGGAAGGTATTTCAGCACAAAAACACCATTTTGGGAGCCTTTCAGAGCTCATTACTCCCATTGA

Coding sequence (CDS)

ATGTTGCCAAAATTTGATCCCAACAACAATGAATCTGGGTTGAAGCTGCTTGAAGATCTTACACAAAATGCAAAGCAAATTCAAGAACAAGTGATTGAGAAGATTCTTACACAAAACTTCAACACAGAATATCTCAAGACTTTTCTCAATGGCCAATCTCCTGATCTCCAAAGTTTCAAGAACTCTGTTCCTGTGGTCAACTATGAAGACATTAAGCCTTATATTGAGAGAATCGCCAATGGTGAACCTTCTCATATAATTTCATCTCAACCCATCACTGAGCTACTCACAAGCTCTGGAACTTCTGGAGGACAGCCAAAAATGATGCCCTCAACTGCTGAGGATTTGGACAGAAAGACATTCTTTTACAATCTCCTTGTACCTGTTTTGAACAAGTATGTGGATGGTTTGGAAGAAGGAAAAGGGATGTATCTTTTGTTTATAAAGCCAGAAATGAGTACCCCATGTGGGTTAATGGCAAGGCCAGTTTTGACAAGTTATTACAAAAGCAAAAACTTCAGAAATAGGCCTTTTAATAGATACAATGTGTACACAAGCCCAGATGAGACAATTCTTTGTTCTGACAGTAAACAGAGTATGTACTGTCAATTGCTTTGTGGGTTATTACAAAGAGATGAAGTTTTAAGAGTTGGTGCAGTTTTTGCCTCTGCTTTTCTTCGAGCTATCAAATTCTTAGAAGATTATTGGAAAGAATTGAGTGATAATATAAGAAATGGGGAAGTTAGTGAATGGATTACTGACTCAAATTGCAGAGCTTCTGTTTCTTTAATCCTTACAAAACCAAATGCTGCTTTGGCTGATTTGATTGATGGTATGTGTGATGAGAAATCATGGGAAGGAATAATTAAGAAGATTTGGCCTAAAACTAAATACATTGAAGTTATTGTAACTGGGTCTATGGCTCAGTATATTCCTACTCTTGAATTTTACAGTGGTGGGCTTCCTTTAGTTTCTACTATGTATGCTTCTTCTGAGTGTTATTTTGGGATTAATTTCAATCCTTTGAGTAAACCTTGTGATGTTTCTTATACACTTCTTCCAAATATGGCCTTCTTTGAATTCTTACCTGTGGAGAAAAATGATGGAGAATTAAGCCATTGCAATGGAAGCTCTGTTCAATCAATTGATGAATTCAAAACAGTTGATCTTGTCGATGTCAAACTTGGTCAATATTACGAATTGGTGGTCACTACTTTCACAGGGTTGTACAGATACAGAGTTGGTGATATTCTAATGGTAACTGGCTTCCACAACAAAGCACCTCAATTCAAGTTTGTTCATAGAAGGAATGTTGTTTTAAGCATAGATACAGATAAGACGAATGAAGATGATTTGTTAAATGCAATTACAAAAGCGAAATTACTTGTTGAACCACTTGGTGTTTTGTTAACAGAGTATACAAGTTATGCAGATACAGGGTCAATTCCAGGGCATTATGTTCTGTTTTGGGAGCTGAAGAAAAGAGGAGGGAATGAGAATTTAGAAGAACTTGAAGTGGGTTTAATGGAAGAATGTTGTTCAAGTATAGAACAATCATTGGATTCAGTTTATAGAAGATGCAGAAGCAAAGATAAATCAATTGGGCCATTGGAGATTAGAGTTGTGAAACATGGAGCTTTTGATGCACTTATGGATTTTTGTGTTTCACAAGGATCTTCTGTGAATCAATACAAAACACCAAGATGCATTAAATCAGAAGAAGCCATCAAAATTCTGGATTCTAGAGTTGTGGGAAGGTATTTCAGCACAAAAACACCATTTTGGGAGCCTTTCAGAGCTCATTACTCCCATTGA

Protein sequence

MLPKFDPNNNESGLKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTFLNGQSPDLQSFKNSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLDRKTFFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNRPFNRYNVYTSPDETILCSDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYWKELSDNIRNGEVSEWITDSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKTKYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMAFFEFLPVEKNDGELSHCNGSSVQSIDEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVGDILMVTGFHNKAPQFKFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTSYADTGSIPGHYVLFWELKKRGGNENLEELEVGLMEECCSSIEQSLDSVYRRCRSKDKSIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFSTKTPFWEPFRAHYSH
BLAST of Lsi07G003990 vs. Swiss-Prot
Match: GH317_ARATH (Indole-3-acetic acid-amido synthetase GH3.17 OS=Arabidopsis thaliana GN=GH3.17 PE=1 SV=1)

HSP 1 Score: 892.1 bits (2304), Expect = 3.3e-258
Identity = 435/605 (71.90%), Postives = 515/605 (85.12%), Query Frame = 1

Query: 1   MLPKFDPNNNESGLKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTFLNGQSP-DLQSF 60
           M+P +DPN+ E+GLKLLEDLT NA+ IQ+QV+ +IL+QN  T+YL+ FL+G++  + QSF
Sbjct: 1   MIPSYDPNDTEAGLKLLEDLTTNAEAIQQQVLHQILSQNSGTQYLRAFLDGEADKNQQSF 60

Query: 61  KNSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLDRK 120
           KN VPVVNY+D+KP+I+RIA+GE S I+S+QPITELLTSSGTS G+PK+MPSTAE+L+RK
Sbjct: 61  KNKVPVVNYDDVKPFIQRIADGESSDIVSAQPITELLTSSGTSAGKPKLMPSTAEELERK 120

Query: 121 TFFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNRPFN 180
           TFFY++LVP++NKYVDGL+EGKGMYLLFIKPE+ TP GLMARPVLTSYYKS++FRNRPFN
Sbjct: 121 TFFYSMLVPIMNKYVDGLDEGKGMYLLFIKPEIKTPSGLMARPVLTSYYKSQHFRNRPFN 180

Query: 181 RYNVYTSPDETILCSDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYWKEL 240
           +YNVYTSPD+TILC DSKQSMYCQLLCGL+QR  VLRVGAVFASAFLRA+KFLED++KEL
Sbjct: 181 KYNVYTSPDQTILCQDSKQSMYCQLLCGLVQRSHVLRVGAVFASAFLRAVKFLEDHYKEL 240

Query: 241 SDNIRNGEVSEWITDSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKTKYI 300
             +IR G V+ WITDS+CR SV  IL  PN  LAD I+  C EKSWEGI+++IWPK KY+
Sbjct: 241 CADIRTGTVTSWITDSSCRDSVLSILNGPNQELADEIESECAEKSWEGILRRIWPKAKYV 300

Query: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMAFF 360
           EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGIN NPL  P DVSYTLLPNMA+F
Sbjct: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINLNPLCDPADVSYTLLPNMAYF 360

Query: 361 EFLPV-EKNDGELSHCNGSSVQSID----EFKTVDLVDVKLGQYYELVVTTFTGLYRYRV 420
           EFLPV +K+  E+     S+    D    E   V+LV+V++GQYYE+V+TTFTGLYRYRV
Sbjct: 361 EFLPVDDKSHEEIHFATHSNTDDDDDALKEDLIVNLVNVEVGQYYEIVITTFTGLYRYRV 420

Query: 421 GDILMVTGFHNKAPQFKFVHRRNVVLSIDTDKTNEDDLLNAITKAKL--LVEPLGVLLTE 480
           GDIL VTGFHNKAPQF+FV RRNVVLSIDTDKT+E+DLLNA+T+AKL  L  P  +LLTE
Sbjct: 421 GDILKVTGFHNKAPQFRFVQRRNVVLSIDTDKTSEEDLLNAVTQAKLNHLQHPSSLLLTE 480

Query: 481 YTSYADTGSIPGHYVLFWELKKRGGNENLEELEVGLMEECCSSIEQSLDSVYRRCRSKDK 540
           YTSYADT SIPGHYVLFWELK R  N+   +L+   ME+CCS +E  LD VYRRCR++DK
Sbjct: 481 YTSYADTSSIPGHYVLFWELKPRHSNDP-PKLDDKTMEDCCSEVEDCLDYVYRRCRNRDK 540

Query: 541 SIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFSTKT 598
           SIGPLEIRVV  G FD+LMDFCVSQGSS+NQYKTPRC+KS  A++ILDSRV+GR+FS + 
Sbjct: 541 SIGPLEIRVVSLGTFDSLMDFCVSQGSSLNQYKTPRCVKSGGALEILDSRVIGRFFSKRV 600

BLAST of Lsi07G003990 vs. Swiss-Prot
Match: GH35_ARATH (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 708.4 bits (1827), Expect = 6.9e-203
Identity = 350/590 (59.32%), Postives = 452/590 (76.61%), Query Frame = 1

Query: 10  NESGLKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTF-LNGQSPDLQSFKNSVPVVNY 69
           N+  L+L+E+LT NA Q+Q QV+E+ILT+N + EYL+   LNG++ D ++FKN +PV+ Y
Sbjct: 21  NKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRT-DRETFKNIMPVITY 80

Query: 70  EDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLDRKTFFYNLLVP 129
           EDI+P I RIANG+ S I+SS+PI+E LTSSGTSGG+ K+MP+  E+LDR++  Y+LL+P
Sbjct: 81  EDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLMP 140

Query: 130 VLNKYVDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNRPFNRYNVYTSPD 189
           V++++V GLE GKGMY LFIK E  TP GL ARPVLTSYYKS +F+ RP++ Y  YTSP+
Sbjct: 141 VMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNYTSPN 200

Query: 190 ETILCSDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYWKELSDNIRNGEV 249
           ETILCSDS QSMY Q+LCGL Q  EVLRVGAVFAS F+RAIKFLE +W EL  +IR G +
Sbjct: 201 ETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIRTGTL 260

Query: 250 SEWITDSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKTKYIEVIVTGSMA 309
           S  ITD + R +V+ IL KP+  LAD ++  C + SW+GII ++WP TKY++VIVTG+M+
Sbjct: 261 SSLITDPSVREAVAKIL-KPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTGTMS 320

Query: 310 QYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMAFFEFLPVEKND 369
           QYIPTL++YS GLPLV TMYASSECYFG+N  PL KP +VSYTL+P+MA+FEFLPV +N+
Sbjct: 321 QYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPVHRNN 380

Query: 370 GELSHCN-GSSVQSIDEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVGDILMVTGFHNKA 429
           G  +  N   ++   ++ + VDLVDVKLGQ YELVVTT+ GL RYRVGD+L VTGF NKA
Sbjct: 381 GVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVTGFKNKA 440

Query: 430 PQFKFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTSYADTGSIPGHYV 489
           PQF F+ R+NVVLSID+DKT+E +L NA+  A   + P    L+EYTSYADT SIPGHYV
Sbjct: 441 PQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYV 500

Query: 490 LFWELKKRGGNENLEELEVGLMEECCSSIEQSLDSVYRRCRSKDKSIGPLEIRVVKHGAF 549
           LFWEL   G       +   + E+CC ++E+S ++VYR+ R  DKSIGPLEI++V+ G F
Sbjct: 501 LFWELCLDGNT----PIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEIKIVEPGTF 560

Query: 550 DALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFSTKTPFWEP 598
           D LMD+ +S G+S+NQYKTPRC+K    I++L+SRVV  YFS K P W P
Sbjct: 561 DKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWVP 604

BLAST of Lsi07G003990 vs. Swiss-Prot
Match: GH36_ARATH (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana GN=GH3.6 PE=1 SV=1)

HSP 1 Score: 705.7 bits (1820), Expect = 4.4e-202
Identity = 347/590 (58.81%), Postives = 450/590 (76.27%), Query Frame = 1

Query: 10  NESGLKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTF-LNGQSPDLQSFKNSVPVVNY 69
           N++ L+ +ED+T NA  +Q +V+E+IL++N + EYLK   L G++ D ++FK+ +PVV Y
Sbjct: 21  NKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRT-DRETFKHIMPVVTY 80

Query: 70  EDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLDRKTFFYNLLVP 129
           EDI+P I RIANG+ S ++ S PI+E LTSSGTSGG+ K+MP+  E+LDR++  Y+LL+P
Sbjct: 81  EDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLMP 140

Query: 130 VLNKYVDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNRPFNRYNVYTSPD 189
           V++++V GL++GKGMY LFIK E  TP GL ARPVLTSYYKS +F+NRP++ Y  YTSP+
Sbjct: 141 VMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYTSPN 200

Query: 190 ETILCSDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYWKELSDNIRNGEV 249
           +TILCSDS QSMY Q+LCGL Q  EVLRVGAVFAS F+RAIKFLE +W EL+ +IR G +
Sbjct: 201 QTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRTGTL 260

Query: 250 SEWITDSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKTKYIEVIVTGSMA 309
           S  ITDS+ R +V  IL KP+  LAD ++  C + SW+GII ++WP TKY++VIVTG+M+
Sbjct: 261 SSEITDSSVREAVGEIL-KPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGTMS 320

Query: 310 QYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMAFFEFLPVEKND 369
           QYIPTL++YS GLPLV TMYASSECYFG+N  PL KP +VSYTL+PNMA+FEFLPV +N 
Sbjct: 321 QYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHRNS 380

Query: 370 GELSHCN-GSSVQSIDEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVGDILMVTGFHNKA 429
           G  S  +   ++   ++ + VDLVDVKLGQ YELVVTT+ GLYRYRVGD+L V GF N A
Sbjct: 381 GVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFKNNA 440

Query: 430 PQFKFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTSYADTGSIPGHYV 489
           PQF F+ R+NVVLSID+DKT+E +L NA+  A   + P    L+EYTSYADT SIPGHYV
Sbjct: 441 PQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYV 500

Query: 490 LFWELKKRGGNENLEELEVGLMEECCSSIEQSLDSVYRRCRSKDKSIGPLEIRVVKHGAF 549
           LFWEL   G       +   + E+CC +IE+SL+SVYR+ R  DKSIGPLEI++V+ G F
Sbjct: 501 LFWELCLNGNT----PIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESGTF 560

Query: 550 DALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFSTKTPFWEP 598
           D LMD+ +S G+S+NQYKTPRC+K    I++L+SRVV  YFS K P W P
Sbjct: 561 DKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSP 604

BLAST of Lsi07G003990 vs. Swiss-Prot
Match: GH311_ORYSJ (Probable indole-3-acetic acid-amido synthetase GH3.11 OS=Oryza sativa subsp. japonica GN=GH3.11 PE=2 SV=1)

HSP 1 Score: 689.9 bits (1779), Expect = 2.5e-197
Identity = 337/581 (58.00%), Postives = 437/581 (75.22%), Query Frame = 1

Query: 14  LKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTFLNGQSPDLQSFKNSVPVVNYEDIKP 73
           L+ LE LT NAK+ QE ++ KIL +N  TEYL  F+NG S ++ +FK  VPVV Y+ + P
Sbjct: 14  LEELEMLTVNAKEAQELILTKILERNQATEYLSKFMNG-STNISAFKRHVPVVTYDKVHP 73

Query: 74  YIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLDRKTFFYNLLVPVLNKY 133
           YI RIA GE S I+  + I ELL SSGTS G+P++MPS  +DLDR+T+ Y+L++P++NKY
Sbjct: 74  YILRIATGEESSILCGEYILELLRSSGTSRGEPRLMPSILKDLDRRTYLYSLIMPIMNKY 133

Query: 134 VDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNRPFNRYNVYTSPDETILC 193
           + GL EGK MYLLF+K E  T  G+  R VLTSYYKS +F +R  + YN YTSPDE ILC
Sbjct: 134 ISGLGEGKAMYLLFVKAETLTDSGIPVRSVLTSYYKSPHFLHRKHDLYNNYTSPDEVILC 193

Query: 194 SDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYWKELSDNIRNGEVSEWIT 253
            DS+QSMYCQLLCGL++R  VLR+GAVFASAFLR+I FLE +W++L ++IR G+++  IT
Sbjct: 194 PDSQQSMYCQLLCGLVERQHVLRIGAVFASAFLRSISFLEQHWRDLVNDIRIGQLNSSIT 253

Query: 254 DSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKTKYIEVIVTGSMAQYIPT 313
              CR ++   L  PN  LAD ++ +C   SW+GI+ ++WP  KYIE ++TG+MAQYIP 
Sbjct: 254 SPACRLAMLNFLALPNPELADQVEAICSCGSWKGILGRLWPNVKYIEAVLTGTMAQYIPM 313

Query: 314 LEFYSGG-LPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMAFFEFLPVEKNDGELS 373
           LEFY GG +P V TMYASSE YFG+N +PL  P DVSYT+LPNMA+FEF+P+E       
Sbjct: 314 LEFYGGGAIPFVCTMYASSESYFGVNLSPLCSPADVSYTILPNMAYFEFIPLEDGLRLTD 373

Query: 374 HCNGSSVQSIDEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVGDILMVTGFHNKAPQFKF 433
           H      + I+  K V LVDVK+G YYELVVTTF+GLYRYRVGD+L VTGF+N+APQFKF
Sbjct: 374 H-----EEVIENDKLVSLVDVKVGCYYELVVTTFSGLYRYRVGDVLQVTGFYNRAPQFKF 433

Query: 434 VHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTSYADTGSIPGHYVLFWEL 493
           + RRNV+LSID+DKTNE+DL N++T AK ++E    LL EYTSY D  ++PGHYVLFWE+
Sbjct: 434 ICRRNVILSIDSDKTNEEDLHNSVTTAKKILENQNYLLLEYTSYTDISTVPGHYVLFWEI 493

Query: 494 KKRGGNENLEELEVGLMEECCSSIEQSLDSVYRRCRSKDKSIGPLEIRVVKHGAFDALMD 553
           K    +E    L+  L+E CC+++E+SLD VYRRCR+ D+SIGPLEIR+V+ GAFDALMD
Sbjct: 494 KST-HDERPAPLDAQLLESCCAAVEESLDYVYRRCRAHDRSIGPLEIRLVEAGAFDALMD 553

Query: 554 FCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFSTKTP 594
             VS GSS+NQYKTPRCI+S  A+K+L+S+V+  +FS + P
Sbjct: 554 LLVSHGSSINQYKTPRCIESSLALKLLNSKVIACFFSPQDP 587

BLAST of Lsi07G003990 vs. Swiss-Prot
Match: GH39_ARATH (Putative indole-3-acetic acid-amido synthetase GH3.9 OS=Arabidopsis thaliana GN=GH3.9 PE=2 SV=1)

HSP 1 Score: 683.7 bits (1763), Expect = 1.8e-195
Identity = 339/593 (57.17%), Postives = 440/593 (74.20%), Query Frame = 1

Query: 9   NNESGLKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTFLNGQSPDLQSFKNSVPVVNY 68
           +++S LK LE +T  A ++Q+ ++  IL +N +TEYL  ++NG S D+  FK +VP++ Y
Sbjct: 7   DHDSVLKELERITSKAAEVQDNILRGILERNKDTEYLSKYMNG-SKDVLEFKRAVPIIIY 66

Query: 69  EDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLDRKTFFYNLLVP 128
           +DI PYI+RIANGE S +I+   ITE+L SSGTS G+PK+MP+  EDLDR+TF YNL++P
Sbjct: 67  KDIYPYIQRIANGEDSSLITGHSITEILCSSGTSAGEPKLMPTIPEDLDRRTFLYNLIIP 126

Query: 129 VLNKYVDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNRPFNRYNVYTSPD 188
           ++NKY+ GL++GK MYL F+K E STPCGL  R VLTSYYKSK+F+ RP++ +N  TSP 
Sbjct: 127 IVNKYITGLDKGKAMYLNFVKAETSTPCGLPIRAVLTSYYKSKHFQCRPYDPFNDLTSPI 186

Query: 189 ETILCSDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYWKELSDNIRNGEV 248
           +TILC DS QSMYCQLL GL+ R +V+R+GAVFASAFLRAI +LE  W +L ++IR G +
Sbjct: 187 QTILCEDSNQSMYCQLLAGLIHRHKVMRLGAVFASAFLRAISYLEKKWSQLCEDIRTGSL 246

Query: 249 SEWITDSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKTKYIEVIVTGSMA 308
           +  ITD  C+ ++S +L  PN  LA  I+ +C   SW+GI+ ++WPK K+IE +VTGSMA
Sbjct: 247 NPMITDPGCQMAMSCLLMSPNPELASEIEEICGRSSWKGILCQLWPKAKFIEAVVTGSMA 306

Query: 309 QYIPTLEFYS-GGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMAFFEFLPVEKN 368
           QYIP LEF+S G +PLV  MYASSE YFG+N  PLSKP DV +TLLPNM +FEF+P+ KN
Sbjct: 307 QYIPALEFFSQGKIPLVCPMYASSETYFGVNVEPLSKPSDVVFTLLPNMCYFEFIPLGKN 366

Query: 369 DGELSHCNGSSVQSIDEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVGDILMVTGFHNKA 428
            G LS  +    + +   K VDLV+VKLG+YYELVVTTF GLYRYR+GD+L V GF+N A
Sbjct: 367 -GTLSF-DLDDDEQVPCDKVVDLVNVKLGRYYELVVTTFAGLYRYRIGDVLQVAGFYNGA 426

Query: 429 PQFKFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLG--VLLTEYTSYADTGSIPGH 488
           PQF+F+ RRNVVLSID DKTNE+DL  +IT AK   + LG    L EYTSYADT S+PGH
Sbjct: 427 PQFRFICRRNVVLSIDLDKTNEEDLHRSITLAK---KKLGSNAFLAEYTSYADTSSVPGH 486

Query: 489 YVLFWELKKRGGNENLEELEVGLMEECCSSIEQSLDSVYRRCRSKDKSIGPLEIRVVKHG 548
           YVLFWE++          LE  LMEECC ++E+ LD +YR+CR+K++SIG LEIRVVK G
Sbjct: 487 YVLFWEIQ--------GHLEPKLMEECCVAVEEELDYIYRQCRTKERSIGALEIRVVKPG 546

Query: 549 AFDALMDFCVSQGSSVNQYKTPRCIKSEEA-IKILDSRVVGRYFSTKTPFWEP 598
            F+ LMD  +SQG S NQYKTPRC+KS  A  K+L+  V+  +FS + P W P
Sbjct: 547 TFEKLMDLIISQGGSFNQYKTPRCVKSNSATFKLLNGHVMASFFSPRDPTWVP 585

BLAST of Lsi07G003990 vs. TrEMBL
Match: A0A0A0KVQ0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G007100 PE=4 SV=1)

HSP 1 Score: 1115.1 bits (2883), Expect = 0.0e+00
Identity = 554/611 (90.67%), Postives = 576/611 (94.27%), Query Frame = 1

Query: 1   MLPKFDPNNNESGLKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTFLNGQ---SPDLQ 60
           MLP FDPNNNESGLKLLEDLT NAKQIQEQVI+KIL QN NTEYLK+FL+     S DLQ
Sbjct: 1   MLPNFDPNNNESGLKLLEDLTTNAKQIQEQVIQKILIQNSNTEYLKSFLHNHHSHSLDLQ 60

Query: 61  SFKNSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLD 120
           +FK+SVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLD
Sbjct: 61  TFKHSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLD 120

Query: 121 RKTFFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNRP 180
           RKTFFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTP GLMARPVLTSYYKSKNFRNRP
Sbjct: 121 RKTFFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPSGLMARPVLTSYYKSKNFRNRP 180

Query: 181 FNRYNVYTSPDETILCSDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYWK 240
           FN+YNVYTSPDETILCSDSKQSMYCQLLCGL+QRDEVLRVGAVFASAFLRAIKFLEDYWK
Sbjct: 181 FNKYNVYTSPDETILCSDSKQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDYWK 240

Query: 241 ELSDNIRNGEVSEWITDSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKTK 300
           ELSDNIR GE+S+WI+D NCRASVS +LTK N  LADLIDG+C EKSWEGIIKK+WPKTK
Sbjct: 241 ELSDNIRKGELSQWISDPNCRASVSSVLTKSNPVLADLIDGLCGEKSWEGIIKKLWPKTK 300

Query: 301 YIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMA 360
           YIEVIVTGSMAQYIPTLEFYSGGLPL+STMYASSECYFGINFNPLSKP DVSYTLLPNMA
Sbjct: 301 YIEVIVTGSMAQYIPTLEFYSGGLPLISTMYASSECYFGINFNPLSKPSDVSYTLLPNMA 360

Query: 361 FFEFLPVEKNDGELSHCNGSSVQSI-DEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVGD 420
           FFEFLPVEKNDGELSHCNG++  S+ +EFKTVDLVDVKLGQYYELVVTTFTGLYRYRVGD
Sbjct: 361 FFEFLPVEKNDGELSHCNGTTNTSVQEEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVGD 420

Query: 421 ILMVTGFHNKAPQFKFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTSY 480
           IL VTGFHNKAPQFKF+HRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTSY
Sbjct: 421 ILKVTGFHNKAPQFKFMHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTSY 480

Query: 481 ADTGSIPGHYVLFWELKKRGGN--ENLEEL-EVGLMEECCSSIEQSLDSVYRRCRSKDKS 540
           ADT SIPGHYVLFWE+KKR G+  EN EEL EV LMEECCSSIE+SLDSVYRRCRSKDKS
Sbjct: 481 ADTESIPGHYVLFWEMKKRSGSNYENFEELVEVDLMEECCSSIEESLDSVYRRCRSKDKS 540

Query: 541 IGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFSTKTP 600
           IGPLEIRVVK GAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVV RYFS KTP
Sbjct: 541 IGPLEIRVVKQGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVARYFSKKTP 600

Query: 601 FWEPFRAHYSH 605
           FWEPFR  YSH
Sbjct: 601 FWEPFRGQYSH 611

BLAST of Lsi07G003990 vs. TrEMBL
Match: M5XVQ6_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa003065mg PE=4 SV=1)

HSP 1 Score: 983.8 bits (2542), Expect = 9.4e-284
Identity = 479/605 (79.17%), Postives = 538/605 (88.93%), Query Frame = 1

Query: 1   MLPKFDPNNNESGLKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTFLNGQSPDLQSFK 60
           M+P ++PN+NE GLKLLEDLT NA ++Q+QV+E+ILT+N  TEYL  FLN    D + FK
Sbjct: 1   MIPSYNPNDNEYGLKLLEDLTTNAYEVQQQVLEEILTKNAQTEYLSAFLNCHY-DRKDFK 60

Query: 61  NSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLDRKT 120
             VPVVNYED+KPYIERIANGEPS IIS+Q ITELLTSSGTSGGQPKMMPST EDLDRKT
Sbjct: 61  KKVPVVNYEDVKPYIERIANGEPSDIISAQKITELLTSSGTSGGQPKMMPSTVEDLDRKT 120

Query: 121 FFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNRPFNR 180
           FFYNLLVPV+NKYVDGL++GKGMYLLFIKPE+STP GL+ARPVLTSYYKS NFRNRPFNR
Sbjct: 121 FFYNLLVPVMNKYVDGLDQGKGMYLLFIKPEISTPSGLVARPVLTSYYKSSNFRNRPFNR 180

Query: 181 YNVYTSPDETILCSDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYWKELS 240
           YN+YTSPDE ILCSDSKQSMYCQLLCGL+QRDEV+RVGAVFASAFLRAIKFLED+WKEL 
Sbjct: 181 YNIYTSPDEIILCSDSKQSMYCQLLCGLVQRDEVMRVGAVFASAFLRAIKFLEDHWKELC 240

Query: 241 DNIRNGEVSEWITDSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKTKYIE 300
            NI +G VSEWITD +CR +VSL+L+KPN  LA+LI    + KSWEGI+K++WP+TKYIE
Sbjct: 241 SNIGSGHVSEWITDPSCRNAVSLVLSKPNPDLANLIKHEFNNKSWEGIVKRLWPRTKYIE 300

Query: 301 VIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMAFFE 360
           VIVTGSMAQYIPTLEFYSGGLPL+STMYASSECYFGINFNPLSKP DV+YTLLPNMA+FE
Sbjct: 301 VIVTGSMAQYIPTLEFYSGGLPLISTMYASSECYFGINFNPLSKPNDVAYTLLPNMAYFE 360

Query: 361 FLPVEKNDGELSHCNGSSVQSI------DEFKTVDLVDVKLGQYYELVVTTFTGLYRYRV 420
           FLPVEK+  E++ CNG S QS       +  +TVDLVDVKLG YYELVVTTFTGLYRYRV
Sbjct: 361 FLPVEKHHEEVALCNGVSDQSCIKEEEKENLETVDLVDVKLGHYYELVVTTFTGLYRYRV 420

Query: 421 GDILMVTGFHNKAPQFKFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYT 480
           GDILMVTGFHNKAPQF+F+HRRNVVLSIDTDKTNE+DLLNA+T+AKLL++PLG LL EYT
Sbjct: 421 GDILMVTGFHNKAPQFRFMHRRNVVLSIDTDKTNEEDLLNAVTQAKLLLDPLGFLLIEYT 480

Query: 481 SYADTGSIPGHYVLFWELKKRGGNENLEELEVGLMEECCSSIEQSLDSVYRRCRSKDKSI 540
           SYADT SIPGHYVLFWELK +  N+ + EL+  +ME+CCS++E+SLDSVYRRCR KD SI
Sbjct: 481 SYADTSSIPGHYVLFWELKTKEKNDFI-ELDTNIMEQCCSTVEESLDSVYRRCRRKDNSI 540

Query: 541 GPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFSTKTPF 600
           GPLEIR+VKHGAFDALMDF VSQGSSVNQYKTPRCIKSEEAIKILDSRVVGR+FS  TP 
Sbjct: 541 GPLEIRIVKHGAFDALMDFSVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRFFSKSTPL 600

BLAST of Lsi07G003990 vs. TrEMBL
Match: B9GMV3_POPTR (Auxin-responsive GH3 family protein OS=Populus trichocarpa GN=POPTR_0001s12850g PE=4 SV=1)

HSP 1 Score: 981.5 bits (2536), Expect = 4.7e-283
Identity = 483/605 (79.83%), Postives = 535/605 (88.43%), Query Frame = 1

Query: 1   MLPKFDPNNNESGLKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTFLNGQSPDLQSFK 60
           MLP FDPN+NE+GLKLLEDLT NA QIQ+QV+E+IL  N +TEYLK+FLNG S   ++FK
Sbjct: 1   MLPVFDPNDNEAGLKLLEDLTNNACQIQQQVLEEILATNLHTEYLKSFLNGDSGK-ENFK 60

Query: 61  NSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLDRKT 120
           N VP+VNYEDIKP IERIANGEPS IIS+QPITELLTSSGTSGGQPK+MPSTAE+L+RKT
Sbjct: 61  NKVPIVNYEDIKPCIERIANGEPSSIISTQPITELLTSSGTSGGQPKIMPSTAEELERKT 120

Query: 121 FFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNRPFNR 180
           FFYNLLVPV+NKYVDGL++GK MYLLFIKPE+STP GLMARPVLTSYYKS NFRNR FNR
Sbjct: 121 FFYNLLVPVMNKYVDGLDQGKAMYLLFIKPEISTPSGLMARPVLTSYYKSNNFRNRAFNR 180

Query: 181 YNVYTSPDETILCSDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYWKELS 240
           YNVYTSPDETILCSDSKQSMYCQLLCGL+QRDEVLRVGA+FASAFLRAIKFLE+Y KEL 
Sbjct: 181 YNVYTSPDETILCSDSKQSMYCQLLCGLVQRDEVLRVGAIFASAFLRAIKFLEEYLKELC 240

Query: 241 DNIRNGEVSEWITDSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKTKYIE 300
            NIR G VS+WITD NCR +V  IL+KPN+ LADLI+  C  KSWEGIIKK+WP+TKYIE
Sbjct: 241 SNIRTGSVSDWITDPNCRNAVLSILSKPNSELADLIEDECSGKSWEGIIKKLWPRTKYIE 300

Query: 301 VIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMAFFE 360
           VIVTGSMAQYIPTLEFYSGGLPLVSTMY SSECY GINF PLS P DVSYTLLPNMA+FE
Sbjct: 301 VIVTGSMAQYIPTLEFYSGGLPLVSTMYGSSECYLGINFKPLSNPYDVSYTLLPNMAYFE 360

Query: 361 FLPVEKNDGEL---SHCNGSSV---QSIDEFKTVDLVDVKLGQYYELVVTTFTGLYRYRV 420
           FLPV+ N  E+     CNG SV      ++ + VDLVDVKLG YYEL+VTTFTGLYRYRV
Sbjct: 361 FLPVDNNHKEVVPDVQCNGVSVTDRNGKEKVEAVDLVDVKLGHYYELIVTTFTGLYRYRV 420

Query: 421 GDILMVTGFHNKAPQFKFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYT 480
           GDILMVTGF+N APQF+FVHRRNVVLSIDTDKTNE+DLL A+T+AK+L+EPLG LLTEYT
Sbjct: 421 GDILMVTGFYNNAPQFRFVHRRNVVLSIDTDKTNEEDLLKAVTQAKVLLEPLGFLLTEYT 480

Query: 481 SYADTGSIPGHYVLFWELKKRGGNENLEELEVGLMEECCSSIEQSLDSVYRRCRSKDKSI 540
           S+ADT SIPGHYVLFWELK RG N+ L EL+  +ME+CCS +E+SLDSVYRRCR KDKSI
Sbjct: 481 SFADTASIPGHYVLFWELKMRGTND-LPELDPVIMEQCCSIVEESLDSVYRRCRKKDKSI 540

Query: 541 GPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFSTKTPF 600
           GPLE+RVV HG FDALMDFCVSQGSSVNQYKTPRCIKSEEA+KILDSRVVGR+ S KTPF
Sbjct: 541 GPLEVRVVTHGTFDALMDFCVSQGSSVNQYKTPRCIKSEEALKILDSRVVGRFSSNKTPF 600

BLAST of Lsi07G003990 vs. TrEMBL
Match: W8CFI4_9ROSA (GH3 family protein OS=Malus sieversii PE=2 SV=1)

HSP 1 Score: 973.0 bits (2514), Expect = 1.7e-280
Identity = 473/605 (78.18%), Postives = 536/605 (88.60%), Query Frame = 1

Query: 1   MLPKFDPNNNESGLKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTFLNGQSPDLQSFK 60
           M+P+++PN++E GLKLLEDLT NA ++Q++V+E+I+ +N  TEYLK FLNG   D + FK
Sbjct: 1   MIPRYNPNDSEYGLKLLEDLTTNAYEVQQRVLEEIIVKNAQTEYLKGFLNGHH-DKKDFK 60

Query: 61  NSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLDRKT 120
           N VPVVNYED+KPYIERIANGE S IIS+Q ITELLTSSGTSGGQPKMMPST EDLDRKT
Sbjct: 61  NKVPVVNYEDVKPYIERIANGETSEIISAQKITELLTSSGTSGGQPKMMPSTVEDLDRKT 120

Query: 121 FFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNRPFNR 180
           FFYNLLVPV+NKYVDGL++GKGMYLLFIKPE+STP GL+ARPVLTSYYKS NFRNRPFNR
Sbjct: 121 FFYNLLVPVMNKYVDGLDQGKGMYLLFIKPEISTPSGLVARPVLTSYYKSSNFRNRPFNR 180

Query: 181 YNVYTSPDETILCSDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYWKELS 240
           +N+YTSPDETILCSDSKQSMYCQLLCGL+QRDEVLRVGAVFASAFLRAIKFLED+W+EL 
Sbjct: 181 FNIYTSPDETILCSDSKQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDHWEELC 240

Query: 241 DNIRNGEVSEWITDSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKTKYIE 300
            NI +G VS WITDS+CR +VSL+L+KPN  L DLI      KS EGI+K++WP+TKYI+
Sbjct: 241 SNISSGIVSAWITDSSCRNAVSLVLSKPNPDLGDLIRHEFSNKSMEGIVKRLWPRTKYID 300

Query: 301 VIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMAFFE 360
           VIVTGSMAQYIPTLEFYSGGLPL+STMYASSECYFGINFNPLSKP DV+YTLLPNMA+FE
Sbjct: 301 VIVTGSMAQYIPTLEFYSGGLPLISTMYASSECYFGINFNPLSKPHDVAYTLLPNMAYFE 360

Query: 361 FLPVEKNDGELSHCNGSSVQSI------DEFKTVDLVDVKLGQYYELVVTTFTGLYRYRV 420
           FLPVEK+  E++ CNG S Q        +  +TVDLVDVKLG YYELVVTTFTGLYRYRV
Sbjct: 361 FLPVEKHHEEVAPCNGVSGQDCANADEEENLRTVDLVDVKLGHYYELVVTTFTGLYRYRV 420

Query: 421 GDILMVTGFHNKAPQFKFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYT 480
           GDILMVTGFHNKAPQF+F+HRRNVVLSIDTDKTNE+DLLNA+T+AKLL+EPLG LL EYT
Sbjct: 421 GDILMVTGFHNKAPQFRFMHRRNVVLSIDTDKTNEEDLLNAVTQAKLLLEPLGFLLIEYT 480

Query: 481 SYADTGSIPGHYVLFWELKKRGGNENLEELEVGLMEECCSSIEQSLDSVYRRCRSKDKSI 540
           SYADT SIPGHYVLFWELK +  N+ + ELE  +ME+CCS++EQSLDSVYRRCR KD SI
Sbjct: 481 SYADTSSIPGHYVLFWELKTKENNDTI-ELETIIMEQCCSTVEQSLDSVYRRCRRKDNSI 540

Query: 541 GPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFSTKTPF 600
           GPLEIR+V+HG FDALMDF VSQGSSVNQYKTPRCI+SEEAI+ILDSRVVGR+FS  TP 
Sbjct: 541 GPLEIRIVQHGTFDALMDFSVSQGSSVNQYKTPRCIQSEEAIRILDSRVVGRFFSKSTPL 600

BLAST of Lsi07G003990 vs. TrEMBL
Match: A0A061EBA9_THECC (Auxin-responsive GH3 family protein OS=Theobroma cacao GN=TCM_008297 PE=4 SV=1)

HSP 1 Score: 968.4 bits (2502), Expect = 4.1e-279
Identity = 471/607 (77.59%), Postives = 533/607 (87.81%), Query Frame = 1

Query: 1   MLPKFDPNNNESGLKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTFLNGQSPDLQSFK 60
           MLP +DPN+N++G+K+LEDLT+N  QIQ++V+E+I+T+N +TEYLK FLNGQS D   FK
Sbjct: 41  MLPNYDPNDNKAGMKILEDLTENVYQIQQRVLEEIITRNAHTEYLKGFLNGQS-DKDLFK 100

Query: 61  NSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLDRKT 120
             VPVVNYEDIKPYIERIANG+ S+II+++P+TELLTSSGTSGGQPKMMPSTAEDL RKT
Sbjct: 101 KKVPVVNYEDIKPYIERIANGDSSNIITAEPVTELLTSSGTSGGQPKMMPSTAEDLHRKT 160

Query: 121 FFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNRPFNR 180
           FFYNLLVPV+N+YVDGL++GKGMYLLF+KPE+ TP GLMARPVLTSYYKS NFRNRPFNR
Sbjct: 161 FFYNLLVPVMNQYVDGLDDGKGMYLLFVKPEIKTPSGLMARPVLTSYYKSSNFRNRPFNR 220

Query: 181 YNVYTSPDETILCSDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYWKELS 240
           +NVYTSPDETILCSDSKQSMYCQLLCGL+QR EVLRVGAVFASAFLRAIKFLEDYWKEL 
Sbjct: 221 FNVYTSPDETILCSDSKQSMYCQLLCGLVQRKEVLRVGAVFASAFLRAIKFLEDYWKELC 280

Query: 241 DNIRNGEVSEWITDSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKTKYIE 300
            NIR G VS+WITD +CR  VSLIL+KPN+ LA+LI+  C  KSWEGIIKK+WP+TKYIE
Sbjct: 281 SNIRTGHVSDWITDPSCRKVVSLILSKPNSELANLIEHECSNKSWEGIIKKLWPRTKYIE 340

Query: 301 VIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMAFFE 360
           VIVTGSMAQYI TLEFYSGGLPLVSTMYASSECYFGINF PLSKP DVSYTL+PN+A+FE
Sbjct: 341 VIVTGSMAQYISTLEFYSGGLPLVSTMYASSECYFGINFKPLSKPSDVSYTLVPNVAYFE 400

Query: 361 FLPVEKNDGELS---HCNGSSVQSI-------DEFKTVDLVDVKLGQYYELVVTTFTGLY 420
           FLPV+KN  + +    CNG   ++        ++ +TVDLVDVKLG YYELVVTTFTGLY
Sbjct: 401 FLPVKKNHEDATQDVQCNGICEENCMEEETGKEDIETVDLVDVKLGHYYELVVTTFTGLY 460

Query: 421 RYRVGDILMVTGFHNKAPQFKFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLL 480
           RYRVGDILMVTGFHN APQF+FVHRRNVVLSIDTDKTNE+DLL A+TKAKLL+E LG LL
Sbjct: 461 RYRVGDILMVTGFHNNAPQFRFVHRRNVVLSIDTDKTNEEDLLKAVTKAKLLLESLGFLL 520

Query: 481 TEYTSYADTGSIPGHYVLFWELKKRGGNENLEELEVGLMEECCSSIEQSLDSVYRRCRSK 540
            EYTSYADT SIPGHYVLFWE   +GGN+ L EL+  +M++CCS++E+SLDSVYRRCR K
Sbjct: 521 IEYTSYADTSSIPGHYVLFWEFTNKGGND-LPELDPKIMKQCCSTVEESLDSVYRRCRRK 580

Query: 541 DKSIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFST 598
           D SIGPLEIRVVKHG FDALMDFCVSQGSSVNQYKTPRCIKS EAI ILDS+VVGR+FS 
Sbjct: 581 DNSIGPLEIRVVKHGTFDALMDFCVSQGSSVNQYKTPRCIKSAEAINILDSKVVGRFFSQ 640

BLAST of Lsi07G003990 vs. TAIR10
Match: AT1G28130.1 (AT1G28130.1 Auxin-responsive GH3 family protein)

HSP 1 Score: 892.1 bits (2304), Expect = 1.9e-259
Identity = 435/605 (71.90%), Postives = 515/605 (85.12%), Query Frame = 1

Query: 1   MLPKFDPNNNESGLKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTFLNGQSP-DLQSF 60
           M+P +DPN+ E+GLKLLEDLT NA+ IQ+QV+ +IL+QN  T+YL+ FL+G++  + QSF
Sbjct: 1   MIPSYDPNDTEAGLKLLEDLTTNAEAIQQQVLHQILSQNSGTQYLRAFLDGEADKNQQSF 60

Query: 61  KNSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLDRK 120
           KN VPVVNY+D+KP+I+RIA+GE S I+S+QPITELLTSSGTS G+PK+MPSTAE+L+RK
Sbjct: 61  KNKVPVVNYDDVKPFIQRIADGESSDIVSAQPITELLTSSGTSAGKPKLMPSTAEELERK 120

Query: 121 TFFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNRPFN 180
           TFFY++LVP++NKYVDGL+EGKGMYLLFIKPE+ TP GLMARPVLTSYYKS++FRNRPFN
Sbjct: 121 TFFYSMLVPIMNKYVDGLDEGKGMYLLFIKPEIKTPSGLMARPVLTSYYKSQHFRNRPFN 180

Query: 181 RYNVYTSPDETILCSDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYWKEL 240
           +YNVYTSPD+TILC DSKQSMYCQLLCGL+QR  VLRVGAVFASAFLRA+KFLED++KEL
Sbjct: 181 KYNVYTSPDQTILCQDSKQSMYCQLLCGLVQRSHVLRVGAVFASAFLRAVKFLEDHYKEL 240

Query: 241 SDNIRNGEVSEWITDSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKTKYI 300
             +IR G V+ WITDS+CR SV  IL  PN  LAD I+  C EKSWEGI+++IWPK KY+
Sbjct: 241 CADIRTGTVTSWITDSSCRDSVLSILNGPNQELADEIESECAEKSWEGILRRIWPKAKYV 300

Query: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMAFF 360
           EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGIN NPL  P DVSYTLLPNMA+F
Sbjct: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINLNPLCDPADVSYTLLPNMAYF 360

Query: 361 EFLPV-EKNDGELSHCNGSSVQSID----EFKTVDLVDVKLGQYYELVVTTFTGLYRYRV 420
           EFLPV +K+  E+     S+    D    E   V+LV+V++GQYYE+V+TTFTGLYRYRV
Sbjct: 361 EFLPVDDKSHEEIHFATHSNTDDDDDALKEDLIVNLVNVEVGQYYEIVITTFTGLYRYRV 420

Query: 421 GDILMVTGFHNKAPQFKFVHRRNVVLSIDTDKTNEDDLLNAITKAKL--LVEPLGVLLTE 480
           GDIL VTGFHNKAPQF+FV RRNVVLSIDTDKT+E+DLLNA+T+AKL  L  P  +LLTE
Sbjct: 421 GDILKVTGFHNKAPQFRFVQRRNVVLSIDTDKTSEEDLLNAVTQAKLNHLQHPSSLLLTE 480

Query: 481 YTSYADTGSIPGHYVLFWELKKRGGNENLEELEVGLMEECCSSIEQSLDSVYRRCRSKDK 540
           YTSYADT SIPGHYVLFWELK R  N+   +L+   ME+CCS +E  LD VYRRCR++DK
Sbjct: 481 YTSYADTSSIPGHYVLFWELKPRHSNDP-PKLDDKTMEDCCSEVEDCLDYVYRRCRNRDK 540

Query: 541 SIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFSTKT 598
           SIGPLEIRVV  G FD+LMDFCVSQGSS+NQYKTPRC+KS  A++ILDSRV+GR+FS + 
Sbjct: 541 SIGPLEIRVVSLGTFDSLMDFCVSQGSSLNQYKTPRCVKSGGALEILDSRVIGRFFSKRV 600

BLAST of Lsi07G003990 vs. TAIR10
Match: AT4G27260.1 (AT4G27260.1 Auxin-responsive GH3 family protein)

HSP 1 Score: 708.4 bits (1827), Expect = 3.9e-204
Identity = 350/590 (59.32%), Postives = 452/590 (76.61%), Query Frame = 1

Query: 10  NESGLKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTF-LNGQSPDLQSFKNSVPVVNY 69
           N+  L+L+E+LT NA Q+Q QV+E+ILT+N + EYL+   LNG++ D ++FKN +PV+ Y
Sbjct: 21  NKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRT-DRETFKNIMPVITY 80

Query: 70  EDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLDRKTFFYNLLVP 129
           EDI+P I RIANG+ S I+SS+PI+E LTSSGTSGG+ K+MP+  E+LDR++  Y+LL+P
Sbjct: 81  EDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLMP 140

Query: 130 VLNKYVDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNRPFNRYNVYTSPD 189
           V++++V GLE GKGMY LFIK E  TP GL ARPVLTSYYKS +F+ RP++ Y  YTSP+
Sbjct: 141 VMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNYTSPN 200

Query: 190 ETILCSDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYWKELSDNIRNGEV 249
           ETILCSDS QSMY Q+LCGL Q  EVLRVGAVFAS F+RAIKFLE +W EL  +IR G +
Sbjct: 201 ETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIRTGTL 260

Query: 250 SEWITDSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKTKYIEVIVTGSMA 309
           S  ITD + R +V+ IL KP+  LAD ++  C + SW+GII ++WP TKY++VIVTG+M+
Sbjct: 261 SSLITDPSVREAVAKIL-KPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTGTMS 320

Query: 310 QYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMAFFEFLPVEKND 369
           QYIPTL++YS GLPLV TMYASSECYFG+N  PL KP +VSYTL+P+MA+FEFLPV +N+
Sbjct: 321 QYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPVHRNN 380

Query: 370 GELSHCN-GSSVQSIDEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVGDILMVTGFHNKA 429
           G  +  N   ++   ++ + VDLVDVKLGQ YELVVTT+ GL RYRVGD+L VTGF NKA
Sbjct: 381 GVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVTGFKNKA 440

Query: 430 PQFKFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTSYADTGSIPGHYV 489
           PQF F+ R+NVVLSID+DKT+E +L NA+  A   + P    L+EYTSYADT SIPGHYV
Sbjct: 441 PQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYV 500

Query: 490 LFWELKKRGGNENLEELEVGLMEECCSSIEQSLDSVYRRCRSKDKSIGPLEIRVVKHGAF 549
           LFWEL   G       +   + E+CC ++E+S ++VYR+ R  DKSIGPLEI++V+ G F
Sbjct: 501 LFWELCLDGNT----PIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEIKIVEPGTF 560

Query: 550 DALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFSTKTPFWEP 598
           D LMD+ +S G+S+NQYKTPRC+K    I++L+SRVV  YFS K P W P
Sbjct: 561 DKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWVP 604

BLAST of Lsi07G003990 vs. TAIR10
Match: AT5G54510.1 (AT5G54510.1 Auxin-responsive GH3 family protein)

HSP 1 Score: 705.7 bits (1820), Expect = 2.5e-203
Identity = 347/590 (58.81%), Postives = 450/590 (76.27%), Query Frame = 1

Query: 10  NESGLKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTF-LNGQSPDLQSFKNSVPVVNY 69
           N++ L+ +ED+T NA  +Q +V+E+IL++N + EYLK   L G++ D ++FK+ +PVV Y
Sbjct: 21  NKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRT-DRETFKHIMPVVTY 80

Query: 70  EDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLDRKTFFYNLLVP 129
           EDI+P I RIANG+ S ++ S PI+E LTSSGTSGG+ K+MP+  E+LDR++  Y+LL+P
Sbjct: 81  EDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLMP 140

Query: 130 VLNKYVDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNRPFNRYNVYTSPD 189
           V++++V GL++GKGMY LFIK E  TP GL ARPVLTSYYKS +F+NRP++ Y  YTSP+
Sbjct: 141 VMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYTSPN 200

Query: 190 ETILCSDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYWKELSDNIRNGEV 249
           +TILCSDS QSMY Q+LCGL Q  EVLRVGAVFAS F+RAIKFLE +W EL+ +IR G +
Sbjct: 201 QTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRTGTL 260

Query: 250 SEWITDSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKTKYIEVIVTGSMA 309
           S  ITDS+ R +V  IL KP+  LAD ++  C + SW+GII ++WP TKY++VIVTG+M+
Sbjct: 261 SSEITDSSVREAVGEIL-KPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGTMS 320

Query: 310 QYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMAFFEFLPVEKND 369
           QYIPTL++YS GLPLV TMYASSECYFG+N  PL KP +VSYTL+PNMA+FEFLPV +N 
Sbjct: 321 QYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHRNS 380

Query: 370 GELSHCN-GSSVQSIDEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVGDILMVTGFHNKA 429
           G  S  +   ++   ++ + VDLVDVKLGQ YELVVTT+ GLYRYRVGD+L V GF N A
Sbjct: 381 GVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFKNNA 440

Query: 430 PQFKFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTSYADTGSIPGHYV 489
           PQF F+ R+NVVLSID+DKT+E +L NA+  A   + P    L+EYTSYADT SIPGHYV
Sbjct: 441 PQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYV 500

Query: 490 LFWELKKRGGNENLEELEVGLMEECCSSIEQSLDSVYRRCRSKDKSIGPLEIRVVKHGAF 549
           LFWEL   G       +   + E+CC +IE+SL+SVYR+ R  DKSIGPLEI++V+ G F
Sbjct: 501 LFWELCLNGNT----PIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESGTF 560

Query: 550 DALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFSTKTPFWEP 598
           D LMD+ +S G+S+NQYKTPRC+K    I++L+SRVV  YFS K P W P
Sbjct: 561 DKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSP 604

BLAST of Lsi07G003990 vs. TAIR10
Match: AT4G37390.1 (AT4G37390.1 Auxin-responsive GH3 family protein)

HSP 1 Score: 694.9 bits (1792), Expect = 4.4e-200
Identity = 338/586 (57.68%), Postives = 442/586 (75.43%), Query Frame = 1

Query: 14  LKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTFLNGQSPDLQSFKNSVPVVNYEDIKP 73
           LK +E++T+N   +QE+V+ +ILT+N NTEYLK F      D ++FK+ VPVV YED+KP
Sbjct: 24  LKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRFDLDGVVDRKTFKSKVPVVTYEDLKP 83

Query: 74  YIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLDRKTFFYNLLVPVLNKY 133
            I+RI+NG+ S I+SS PITE LTSSGTS G+ K+MP+  EDLDR+   Y+LL+PV+N Y
Sbjct: 84  EIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMPTIEEDLDRRQLLYSLLMPVMNLY 143

Query: 134 VDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNRPFNRYNVYTSPDETILC 193
           V GL++GKG+Y LF+K E  T  GL ARPVLTSYYKS +F+ RP++ YNVYTSP+E ILC
Sbjct: 144 VPGLDKGKGLYFLFVKSESKTSGGLPARPVLTSYYKSDHFKRRPYDPYNVYTSPNEAILC 203

Query: 194 SDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYWKELSDNIRNGEVSEWIT 253
           SDS QSMY Q+LCGLL R EVLR+GAVFAS  LRAI FL++ WKEL+ +I  G +S  I 
Sbjct: 204 SDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAISFLQNNWKELARDISTGTLSSRIF 263

Query: 254 DSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKTKYIEVIVTGSMAQYIPT 313
           D   +  +S ILTKP+  LA+ + G+C +++WEGII KIWP TKY++VIVTG+MAQYIPT
Sbjct: 264 DPAIKNRMSKILTKPDQELAEFLVGVCSQENWEGIITKIWPNTKYLDVIVTGAMAQYIPT 323

Query: 314 LEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMAFFEFLPVEKNDGELSH 373
           LE+YSGGLP+  TMYASSE YFGIN  P+ KP +VSYT++PNMA+FEFLP   +DG+   
Sbjct: 324 LEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLP-HNHDGD--- 383

Query: 374 CNGSSVQSIDEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVGDILMVTGFHNKAPQFKFV 433
             G++  S+DE   V+L +V++G+ YELV+TT+ GLYRYRVGDIL VTGFHN APQFKF+
Sbjct: 384 --GAAEASLDETSLVELANVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAPQFKFI 443

Query: 434 HRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTSYADTGSIPGHYVLFWELK 493
            R+NV+LS+++DKT+E +L  A+  A  L    G  + EYTSYA+T +IPGHYV++WEL 
Sbjct: 444 RRKNVLLSVESDKTDEAELQKAVENASRLFAEQGTRVIEYTSYAETKTIPGHYVIYWELL 503

Query: 494 KRGGNENLEELEVGLMEECCSSIEQSLDSVYRRCRSKDKSIGPLEIRVVKHGAFDALMDF 553
            R  +  L   EV  M +CC  +E+SL+SVYR+ R  DKSIGPLEIRVV++G F+ LMD+
Sbjct: 504 GRDQSNALMSEEV--MAKCCLEMEESLNSVYRQSRVADKSIGPLEIRVVRNGTFEELMDY 563

Query: 554 CVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFSTKTPFWEPFR 600
            +S+G+S+NQYK PRC+     +++LDSRVV  +FS   P W P R
Sbjct: 564 AISRGASINQYKVPRCVSFTPIMELLDSRVVSAHFSPSLPHWSPER 601

BLAST of Lsi07G003990 vs. TAIR10
Match: AT5G13370.1 (AT5G13370.1 Auxin-responsive GH3 family protein)

HSP 1 Score: 686.0 bits (1769), Expect = 2.1e-197
Identity = 341/601 (56.74%), Postives = 434/601 (72.21%), Query Frame = 1

Query: 1   MLPKFDPNNNESGLKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTFLNGQSPDLQSFK 60
           MLPKFDP N ++ L LLEDLT N KQIQ+ V+E IL++N  TEYL+ FLNGQ  D Q+FK
Sbjct: 1   MLPKFDPTNQKACLSLLEDLTTNVKQIQDSVLEAILSRNAQTEYLRGFLNGQV-DKQNFK 60

Query: 61  NSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLDRKT 120
            +VPVV YEDI+ YI+RIANGEPS +I  +PI+ LLTSSGTSGG PK++P T EDL+++ 
Sbjct: 61  KNVPVVTYEDIRSYIDRIANGEPSDLICDRPISVLLTSSGTSGGVPKLIPLTTEDLEQRI 120

Query: 121 FFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNRPFNR 180
            F +L  P+L K++DGL EGK +   F+  E  T  GLM R ++TS+ KS    N  F  
Sbjct: 121 SFSSLYAPLLYKHIDGLSEGKSLIFYFVTRESKTANGLMVRTMVTSFLKSIKQTNS-FLW 180

Query: 181 YNVYTSPDETILCSDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYWKELS 240
            ++  SP     C+D+ QSMYCQLLCGLL+RD V R+GA FAS+FL+ IKFLED+W EL 
Sbjct: 181 DSLQVSPHAITTCADTTQSMYCQLLCGLLERDNVARLGAPFASSFLKVIKFLEDHWPELC 240

Query: 241 DNIRNGEVSEWITDSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKTKYIE 300
            NIR G +S+WITD+ C + +   LT PN  LA LI+  C + SWE I+K++WPK K IE
Sbjct: 241 SNIRTGRLSDWITDATCTSGIGKFLTAPNPELASLIEQECSKTSWEAILKRLWPKAKCIE 300

Query: 301 VIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMAFFE 360
            I+TG+MAQYIP LEFYSGGLPL S+ Y SSEC+ G+NFNPL KP DVSYT++P M +FE
Sbjct: 301 SIITGTMAQYIPLLEFYSGGLPLTSSFYGSSECFMGVNFNPLCKPSDVSYTIIPCMGYFE 360

Query: 361 FLPVEKNDGELSHCNGSSVQSIDEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVGDILMV 420
           FL VEK+  E  H         ++   VDLVDVK+G  YE VVTTF+GLYRYRVGD+L  
Sbjct: 361 FLEVEKDHQEAGH------DPTEKPVVVDLVDVKIGHDYEPVVTTFSGLYRYRVGDVLRA 420

Query: 421 TGFHNKAPQFKFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTSYADTG 480
           TGF+N AP F FV R+ VVLSID DKT EDDLL A+T AKLL+EP  ++L ++TS  D+ 
Sbjct: 421 TGFYNNAPHFCFVGRQKVVLSIDMDKTYEDDLLKAVTNAKLLLEPHDLMLMDFTSRVDSS 480

Query: 481 SIPGHYVLFWELKKRGGNENLEELEVGLMEECCSSIEQSLDSVYRRCRSKDKSIGPLEIR 540
           S PGHYV++WEL  +  +   E     +MEECC ++E+SLD+VYR+ R  DK+IGPLEI+
Sbjct: 481 SFPGHYVIYWELGSKVKDAKFEP-NRDVMEECCFTVEESLDAVYRKGRKNDKNIGPLEIK 540

Query: 541 VVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFSTKTPFWEPFRA 600
           VVK GAFD LM+F +S+GSSV+QYKTPR + +EEA+KIL++ V+  + S K P WE    
Sbjct: 541 VVKPGAFDELMNFFLSRGSSVSQYKTPRSVTNEEALKILEANVISEFLSRKIPSWELHEL 592

Query: 601 H 602
           H
Sbjct: 601 H 592

BLAST of Lsi07G003990 vs. NCBI nr
Match: gi|659108362|ref|XP_008454158.1| (PREDICTED: indole-3-acetic acid-amido synthetase GH3.17-like [Cucumis melo])

HSP 1 Score: 1122.1 bits (2901), Expect = 0.0e+00
Identity = 557/612 (91.01%), Postives = 578/612 (94.44%), Query Frame = 1

Query: 1   MLPKFDPNNNESGLKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTFLNGQ----SPDL 60
           MLP FDPNNNESGLKLLEDLT NAKQIQEQVI KILTQN NTEYLK+FL+      S DL
Sbjct: 1   MLPNFDPNNNESGLKLLEDLTTNAKQIQEQVIRKILTQNSNTEYLKSFLHNHHYSHSLDL 60

Query: 61  QSFKNSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDL 120
           Q+FK+SVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDL
Sbjct: 61  QTFKHSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDL 120

Query: 121 DRKTFFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNR 180
           DRKTFFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTP GLMARPVLTSYYKSKNFRNR
Sbjct: 121 DRKTFFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPSGLMARPVLTSYYKSKNFRNR 180

Query: 181 PFNRYNVYTSPDETILCSDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYW 240
           PFN+YNVYTSPDETILCSDSKQSMYCQLLCGL+QRDEVLRVGAVFASAFLRAIKFLEDYW
Sbjct: 181 PFNKYNVYTSPDETILCSDSKQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDYW 240

Query: 241 KELSDNIRNGEVSEWITDSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKT 300
           KELSDNIR GE+S+WI+D NCRASVSLILTK N  LADLIDG+C EKSWEGIIKK+WPKT
Sbjct: 241 KELSDNIRKGELSQWISDPNCRASVSLILTKSNPVLADLIDGLCGEKSWEGIIKKLWPKT 300

Query: 301 KYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNM 360
           KYIEVIVTGSMAQYIPTLEFYSGGLPL+STMYASSECYFGINFNPLSKP DVSYTLLPNM
Sbjct: 301 KYIEVIVTGSMAQYIPTLEFYSGGLPLISTMYASSECYFGINFNPLSKPSDVSYTLLPNM 360

Query: 361 AFFEFLPVEKNDGELSHCNGSSVQSI-DEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVG 420
           AFFEFLPVEKNDGEL+HCNG++  S+ DEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVG
Sbjct: 361 AFFEFLPVEKNDGELTHCNGTTNTSVQDEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVG 420

Query: 421 DILMVTGFHNKAPQFKFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTS 480
           DIL VTGFHNKAPQFKF+HRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTS
Sbjct: 421 DILKVTGFHNKAPQFKFIHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTS 480

Query: 481 YADTGSIPGHYVLFWELKKRGGN--ENLEEL-EVGLMEECCSSIEQSLDSVYRRCRSKDK 540
           YADT SIPGHYVLFWE+K+R G+  EN EEL EV LMEECCSSIE+SLDSVYRRCRSKDK
Sbjct: 481 YADTESIPGHYVLFWEMKRRSGSNYENFEELVEVDLMEECCSSIEESLDSVYRRCRSKDK 540

Query: 541 SIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFSTKT 600
           SIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVV RYFS KT
Sbjct: 541 SIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVARYFSKKT 600

Query: 601 PFWEPFRAHYSH 605
           PFWEPFR  YSH
Sbjct: 601 PFWEPFRGQYSH 612

BLAST of Lsi07G003990 vs. NCBI nr
Match: gi|449468912|ref|XP_004152165.1| (PREDICTED: indole-3-acetic acid-amido synthetase GH3.17-like [Cucumis sativus])

HSP 1 Score: 1115.1 bits (2883), Expect = 0.0e+00
Identity = 554/611 (90.67%), Postives = 576/611 (94.27%), Query Frame = 1

Query: 1   MLPKFDPNNNESGLKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTFLNGQ---SPDLQ 60
           MLP FDPNNNESGLKLLEDLT NAKQIQEQVI+KIL QN NTEYLK+FL+     S DLQ
Sbjct: 1   MLPNFDPNNNESGLKLLEDLTTNAKQIQEQVIQKILIQNSNTEYLKSFLHNHHSHSLDLQ 60

Query: 61  SFKNSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLD 120
           +FK+SVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLD
Sbjct: 61  TFKHSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLD 120

Query: 121 RKTFFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNRP 180
           RKTFFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTP GLMARPVLTSYYKSKNFRNRP
Sbjct: 121 RKTFFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPSGLMARPVLTSYYKSKNFRNRP 180

Query: 181 FNRYNVYTSPDETILCSDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYWK 240
           FN+YNVYTSPDETILCSDSKQSMYCQLLCGL+QRDEVLRVGAVFASAFLRAIKFLEDYWK
Sbjct: 181 FNKYNVYTSPDETILCSDSKQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDYWK 240

Query: 241 ELSDNIRNGEVSEWITDSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKTK 300
           ELSDNIR GE+S+WI+D NCRASVS +LTK N  LADLIDG+C EKSWEGIIKK+WPKTK
Sbjct: 241 ELSDNIRKGELSQWISDPNCRASVSSVLTKSNPVLADLIDGLCGEKSWEGIIKKLWPKTK 300

Query: 301 YIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMA 360
           YIEVIVTGSMAQYIPTLEFYSGGLPL+STMYASSECYFGINFNPLSKP DVSYTLLPNMA
Sbjct: 301 YIEVIVTGSMAQYIPTLEFYSGGLPLISTMYASSECYFGINFNPLSKPSDVSYTLLPNMA 360

Query: 361 FFEFLPVEKNDGELSHCNGSSVQSI-DEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVGD 420
           FFEFLPVEKNDGELSHCNG++  S+ +EFKTVDLVDVKLGQYYELVVTTFTGLYRYRVGD
Sbjct: 361 FFEFLPVEKNDGELSHCNGTTNTSVQEEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVGD 420

Query: 421 ILMVTGFHNKAPQFKFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTSY 480
           IL VTGFHNKAPQFKF+HRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTSY
Sbjct: 421 ILKVTGFHNKAPQFKFMHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTSY 480

Query: 481 ADTGSIPGHYVLFWELKKRGGN--ENLEEL-EVGLMEECCSSIEQSLDSVYRRCRSKDKS 540
           ADT SIPGHYVLFWE+KKR G+  EN EEL EV LMEECCSSIE+SLDSVYRRCRSKDKS
Sbjct: 481 ADTESIPGHYVLFWEMKKRSGSNYENFEELVEVDLMEECCSSIEESLDSVYRRCRSKDKS 540

Query: 541 IGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFSTKTP 600
           IGPLEIRVVK GAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVV RYFS KTP
Sbjct: 541 IGPLEIRVVKQGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVARYFSKKTP 600

Query: 601 FWEPFRAHYSH 605
           FWEPFR  YSH
Sbjct: 601 FWEPFRGQYSH 611

BLAST of Lsi07G003990 vs. NCBI nr
Match: gi|645231167|ref|XP_008222270.1| (PREDICTED: indole-3-acetic acid-amido synthetase GH3.17-like [Prunus mume])

HSP 1 Score: 991.9 bits (2563), Expect = 5.0e-286
Identity = 482/605 (79.67%), Postives = 541/605 (89.42%), Query Frame = 1

Query: 1   MLPKFDPNNNESGLKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTFLNGQSPDLQSFK 60
           M+P ++PN+NE GLKLLEDLT NA ++Q+QV+E+ILT+N  TEYL  FLNG   D + FK
Sbjct: 5   MIPSYNPNDNEYGLKLLEDLTTNAYEVQQQVLEEILTKNAETEYLSAFLNGHY-DRKDFK 64

Query: 61  NSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLDRKT 120
             VPVV+YED+KPYIERIANGEPS IIS+Q ITELLTSSGTSGGQPKMMPST EDLDRKT
Sbjct: 65  KKVPVVSYEDVKPYIERIANGEPSDIISAQKITELLTSSGTSGGQPKMMPSTVEDLDRKT 124

Query: 121 FFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNRPFNR 180
           FFYNLLVPV+NKYVDGL++GKGMYLLFIKPE+STPCGL+ARPVLTSYYKS NFRNRPFNR
Sbjct: 125 FFYNLLVPVMNKYVDGLDQGKGMYLLFIKPEISTPCGLVARPVLTSYYKSSNFRNRPFNR 184

Query: 181 YNVYTSPDETILCSDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYWKELS 240
           YN+YTSPDE ILCSDSKQSMYCQLLCGL+QRDEVLRVGAVFASAFLRAIKFLED+WKEL 
Sbjct: 185 YNIYTSPDEIILCSDSKQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDHWKELC 244

Query: 241 DNIRNGEVSEWITDSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKTKYIE 300
            NI +G VS+WITD +CR +VSL+L+KPN  LA+LI+   + KSWEGI+K++WP+TKYIE
Sbjct: 245 SNIGSGHVSDWITDLSCRNAVSLVLSKPNPDLANLIEHEFNNKSWEGIVKRLWPRTKYIE 304

Query: 301 VIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMAFFE 360
           VIVTGSMAQYIPTLEFYSGGLPL+STMYASSECYFGINFNPLSKP DV+YTLLPNMA+FE
Sbjct: 305 VIVTGSMAQYIPTLEFYSGGLPLISTMYASSECYFGINFNPLSKPHDVAYTLLPNMAYFE 364

Query: 361 FLPVEKNDGELSHCNGSSVQSI------DEFKTVDLVDVKLGQYYELVVTTFTGLYRYRV 420
           FLPVEK+  E++ CNG S QS       +  +TVDLVDVKLG YYELVVTTFTGLYRYRV
Sbjct: 365 FLPVEKHHEEVALCNGVSDQSCIKEEEKENLETVDLVDVKLGHYYELVVTTFTGLYRYRV 424

Query: 421 GDILMVTGFHNKAPQFKFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYT 480
           GDILMVTGFHNKAPQF+F+HRRNVVLSIDTDKTNE+DLLNA+TKAKLL++PLG LL EYT
Sbjct: 425 GDILMVTGFHNKAPQFRFMHRRNVVLSIDTDKTNEEDLLNAVTKAKLLLDPLGFLLIEYT 484

Query: 481 SYADTGSIPGHYVLFWELKKRGGNENLEELEVGLMEECCSSIEQSLDSVYRRCRSKDKSI 540
           SYADT SIPGHYVLFWELK +  N+ L EL+  +ME+CCS++E+SLDSVYRRCR KD SI
Sbjct: 485 SYADTSSIPGHYVLFWELKTKEKND-LIELDTNIMEQCCSTVEESLDSVYRRCRRKDNSI 544

Query: 541 GPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFSTKTPF 600
           GPLEIR+VKHGAFDALMDF VSQGSSVNQYKTPRCIKSEEAIKILDSRVVGR+FS  TP 
Sbjct: 545 GPLEIRIVKHGAFDALMDFSVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRFFSKSTPL 604

BLAST of Lsi07G003990 vs. NCBI nr
Match: gi|596127653|ref|XP_007222041.1| (hypothetical protein PRUPE_ppa003065mg [Prunus persica])

HSP 1 Score: 983.8 bits (2542), Expect = 1.3e-283
Identity = 479/605 (79.17%), Postives = 538/605 (88.93%), Query Frame = 1

Query: 1   MLPKFDPNNNESGLKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTFLNGQSPDLQSFK 60
           M+P ++PN+NE GLKLLEDLT NA ++Q+QV+E+ILT+N  TEYL  FLN    D + FK
Sbjct: 1   MIPSYNPNDNEYGLKLLEDLTTNAYEVQQQVLEEILTKNAQTEYLSAFLNCHY-DRKDFK 60

Query: 61  NSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLDRKT 120
             VPVVNYED+KPYIERIANGEPS IIS+Q ITELLTSSGTSGGQPKMMPST EDLDRKT
Sbjct: 61  KKVPVVNYEDVKPYIERIANGEPSDIISAQKITELLTSSGTSGGQPKMMPSTVEDLDRKT 120

Query: 121 FFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNRPFNR 180
           FFYNLLVPV+NKYVDGL++GKGMYLLFIKPE+STP GL+ARPVLTSYYKS NFRNRPFNR
Sbjct: 121 FFYNLLVPVMNKYVDGLDQGKGMYLLFIKPEISTPSGLVARPVLTSYYKSSNFRNRPFNR 180

Query: 181 YNVYTSPDETILCSDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYWKELS 240
           YN+YTSPDE ILCSDSKQSMYCQLLCGL+QRDEV+RVGAVFASAFLRAIKFLED+WKEL 
Sbjct: 181 YNIYTSPDEIILCSDSKQSMYCQLLCGLVQRDEVMRVGAVFASAFLRAIKFLEDHWKELC 240

Query: 241 DNIRNGEVSEWITDSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKTKYIE 300
            NI +G VSEWITD +CR +VSL+L+KPN  LA+LI    + KSWEGI+K++WP+TKYIE
Sbjct: 241 SNIGSGHVSEWITDPSCRNAVSLVLSKPNPDLANLIKHEFNNKSWEGIVKRLWPRTKYIE 300

Query: 301 VIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMAFFE 360
           VIVTGSMAQYIPTLEFYSGGLPL+STMYASSECYFGINFNPLSKP DV+YTLLPNMA+FE
Sbjct: 301 VIVTGSMAQYIPTLEFYSGGLPLISTMYASSECYFGINFNPLSKPNDVAYTLLPNMAYFE 360

Query: 361 FLPVEKNDGELSHCNGSSVQSI------DEFKTVDLVDVKLGQYYELVVTTFTGLYRYRV 420
           FLPVEK+  E++ CNG S QS       +  +TVDLVDVKLG YYELVVTTFTGLYRYRV
Sbjct: 361 FLPVEKHHEEVALCNGVSDQSCIKEEEKENLETVDLVDVKLGHYYELVVTTFTGLYRYRV 420

Query: 421 GDILMVTGFHNKAPQFKFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYT 480
           GDILMVTGFHNKAPQF+F+HRRNVVLSIDTDKTNE+DLLNA+T+AKLL++PLG LL EYT
Sbjct: 421 GDILMVTGFHNKAPQFRFMHRRNVVLSIDTDKTNEEDLLNAVTQAKLLLDPLGFLLIEYT 480

Query: 481 SYADTGSIPGHYVLFWELKKRGGNENLEELEVGLMEECCSSIEQSLDSVYRRCRSKDKSI 540
           SYADT SIPGHYVLFWELK +  N+ + EL+  +ME+CCS++E+SLDSVYRRCR KD SI
Sbjct: 481 SYADTSSIPGHYVLFWELKTKEKNDFI-ELDTNIMEQCCSTVEESLDSVYRRCRRKDNSI 540

Query: 541 GPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFSTKTPF 600
           GPLEIR+VKHGAFDALMDF VSQGSSVNQYKTPRCIKSEEAIKILDSRVVGR+FS  TP 
Sbjct: 541 GPLEIRIVKHGAFDALMDFSVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRFFSKSTPL 600

BLAST of Lsi07G003990 vs. NCBI nr
Match: gi|224057862|ref|XP_002299361.1| (auxin-responsive GH3 family protein [Populus trichocarpa])

HSP 1 Score: 981.5 bits (2536), Expect = 6.7e-283
Identity = 483/605 (79.83%), Postives = 535/605 (88.43%), Query Frame = 1

Query: 1   MLPKFDPNNNESGLKLLEDLTQNAKQIQEQVIEKILTQNFNTEYLKTFLNGQSPDLQSFK 60
           MLP FDPN+NE+GLKLLEDLT NA QIQ+QV+E+IL  N +TEYLK+FLNG S   ++FK
Sbjct: 1   MLPVFDPNDNEAGLKLLEDLTNNACQIQQQVLEEILATNLHTEYLKSFLNGDSGK-ENFK 60

Query: 61  NSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDLDRKT 120
           N VP+VNYEDIKP IERIANGEPS IIS+QPITELLTSSGTSGGQPK+MPSTAE+L+RKT
Sbjct: 61  NKVPIVNYEDIKPCIERIANGEPSSIISTQPITELLTSSGTSGGQPKIMPSTAEELERKT 120

Query: 121 FFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPCGLMARPVLTSYYKSKNFRNRPFNR 180
           FFYNLLVPV+NKYVDGL++GK MYLLFIKPE+STP GLMARPVLTSYYKS NFRNR FNR
Sbjct: 121 FFYNLLVPVMNKYVDGLDQGKAMYLLFIKPEISTPSGLMARPVLTSYYKSNNFRNRAFNR 180

Query: 181 YNVYTSPDETILCSDSKQSMYCQLLCGLLQRDEVLRVGAVFASAFLRAIKFLEDYWKELS 240
           YNVYTSPDETILCSDSKQSMYCQLLCGL+QRDEVLRVGA+FASAFLRAIKFLE+Y KEL 
Sbjct: 181 YNVYTSPDETILCSDSKQSMYCQLLCGLVQRDEVLRVGAIFASAFLRAIKFLEEYLKELC 240

Query: 241 DNIRNGEVSEWITDSNCRASVSLILTKPNAALADLIDGMCDEKSWEGIIKKIWPKTKYIE 300
            NIR G VS+WITD NCR +V  IL+KPN+ LADLI+  C  KSWEGIIKK+WP+TKYIE
Sbjct: 241 SNIRTGSVSDWITDPNCRNAVLSILSKPNSELADLIEDECSGKSWEGIIKKLWPRTKYIE 300

Query: 301 VIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMAFFE 360
           VIVTGSMAQYIPTLEFYSGGLPLVSTMY SSECY GINF PLS P DVSYTLLPNMA+FE
Sbjct: 301 VIVTGSMAQYIPTLEFYSGGLPLVSTMYGSSECYLGINFKPLSNPYDVSYTLLPNMAYFE 360

Query: 361 FLPVEKNDGEL---SHCNGSSV---QSIDEFKTVDLVDVKLGQYYELVVTTFTGLYRYRV 420
           FLPV+ N  E+     CNG SV      ++ + VDLVDVKLG YYEL+VTTFTGLYRYRV
Sbjct: 361 FLPVDNNHKEVVPDVQCNGVSVTDRNGKEKVEAVDLVDVKLGHYYELIVTTFTGLYRYRV 420

Query: 421 GDILMVTGFHNKAPQFKFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYT 480
           GDILMVTGF+N APQF+FVHRRNVVLSIDTDKTNE+DLL A+T+AK+L+EPLG LLTEYT
Sbjct: 421 GDILMVTGFYNNAPQFRFVHRRNVVLSIDTDKTNEEDLLKAVTQAKVLLEPLGFLLTEYT 480

Query: 481 SYADTGSIPGHYVLFWELKKRGGNENLEELEVGLMEECCSSIEQSLDSVYRRCRSKDKSI 540
           S+ADT SIPGHYVLFWELK RG N+ L EL+  +ME+CCS +E+SLDSVYRRCR KDKSI
Sbjct: 481 SFADTASIPGHYVLFWELKMRGTND-LPELDPVIMEQCCSIVEESLDSVYRRCRKKDKSI 540

Query: 541 GPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVGRYFSTKTPF 600
           GPLE+RVV HG FDALMDFCVSQGSSVNQYKTPRCIKSEEA+KILDSRVVGR+ S KTPF
Sbjct: 541 GPLEVRVVTHGTFDALMDFCVSQGSSVNQYKTPRCIKSEEALKILDSRVVGRFSSNKTPF 600

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
GH317_ARATH3.3e-25871.90Indole-3-acetic acid-amido synthetase GH3.17 OS=Arabidopsis thaliana GN=GH3.17 P... [more]
GH35_ARATH6.9e-20359.32Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana GN=GH3.5 PE=... [more]
GH36_ARATH4.4e-20258.81Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana GN=GH3.6 PE=... [more]
GH311_ORYSJ2.5e-19758.00Probable indole-3-acetic acid-amido synthetase GH3.11 OS=Oryza sativa subsp. jap... [more]
GH39_ARATH1.8e-19557.17Putative indole-3-acetic acid-amido synthetase GH3.9 OS=Arabidopsis thaliana GN=... [more]
Match NameE-valueIdentityDescription
A0A0A0KVQ0_CUCSA0.0e+0090.67Uncharacterized protein OS=Cucumis sativus GN=Csa_4G007100 PE=4 SV=1[more]
M5XVQ6_PRUPE9.4e-28479.17Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa003065mg PE=4 SV=1[more]
B9GMV3_POPTR4.7e-28379.83Auxin-responsive GH3 family protein OS=Populus trichocarpa GN=POPTR_0001s12850g ... [more]
W8CFI4_9ROSA1.7e-28078.18GH3 family protein OS=Malus sieversii PE=2 SV=1[more]
A0A061EBA9_THECC4.1e-27977.59Auxin-responsive GH3 family protein OS=Theobroma cacao GN=TCM_008297 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G28130.11.9e-25971.90 Auxin-responsive GH3 family protein[more]
AT4G27260.13.9e-20459.32 Auxin-responsive GH3 family protein[more]
AT5G54510.12.5e-20358.81 Auxin-responsive GH3 family protein[more]
AT4G37390.14.4e-20057.68 Auxin-responsive GH3 family protein[more]
AT5G13370.12.1e-19756.74 Auxin-responsive GH3 family protein[more]
Match NameE-valueIdentityDescription
gi|659108362|ref|XP_008454158.1|0.0e+0091.01PREDICTED: indole-3-acetic acid-amido synthetase GH3.17-like [Cucumis melo][more]
gi|449468912|ref|XP_004152165.1|0.0e+0090.67PREDICTED: indole-3-acetic acid-amido synthetase GH3.17-like [Cucumis sativus][more]
gi|645231167|ref|XP_008222270.1|5.0e-28679.67PREDICTED: indole-3-acetic acid-amido synthetase GH3.17-like [Prunus mume][more]
gi|596127653|ref|XP_007222041.1|1.3e-28379.17hypothetical protein PRUPE_ppa003065mg [Prunus persica][more]
gi|224057862|ref|XP_002299361.1|6.7e-28379.83auxin-responsive GH3 family protein [Populus trichocarpa][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR004993GH3
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
biological_process GO:0009733 response to auxin
cellular_component GO:0005575 cellular_component
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0003674 molecular_function
molecular_function GO:0010279 indole-3-acetic acid amido synthetase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi07G003990.1Lsi07G003990.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004993GH3 familyPANTHERPTHR31901FAMILY NOT NAMEDcoord: 9..599
score:
IPR004993GH3 familyPFAMPF03321GH3coord: 17..573
score: 8.4E
NoneNo IPR availableunknownCoilCoilcoord: 10..37
scor
NoneNo IPR availablePANTHERPTHR31901:SF18INDOLE-3-ACETIC ACID-AMIDO SYNTHETASE GH3.9-RELATEDcoord: 9..599
score: