Lsi07G000860 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi07G000860
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionLipid transfer protein
Locationchr07 : 948052 .. 948351 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATAAAGCTATAAAGGTTGTGGCTTTGGCATTGGTCTTGATGGTTGTGATTGGTTTTGGTGAAGCTCAAACCATTTGCAACATGCCAGTTGCTGGTTTGTATGCATGTAGACCGTCGGTGACACCTCCGAACCCGACACCCCCGACCGCTCAATGTTGCATGGCGCTCTCGCACGCCGACTTGCACTGCTTTTGCACTTACCGGAACTCGGGAGCTCTTTCTTCTCTTGGCATTGACCCTAATCTTGCAATGCAGTTACCTAAGATCTGCAAGCTTCCTAACTCTCCAAATTGCTAG

mRNA sequence

ATGGATAAAGCTATAAAGGTTGTGGCTTTGGCATTGGTCTTGATGGTTGTGATTGGTTTTGGTGAAGCTCAAACCATTTGCAACATGCCAGTTGCTGGTTTGTATGCATGTAGACCGTCGGTGACACCTCCGAACCCGACACCCCCGACCGCTCAATGTTGCATGGCGCTCTCGCACGCCGACTTGCACTGCTTTTGCACTTACCGGAACTCGGGAGCTCTTTCTTCTCTTGGCATTGACCCTAATCTTGCAATGCAGTTACCTAAGATCTGCAAGCTTCCTAACTCTCCAAATTGCTAG

Coding sequence (CDS)

ATGGATAAAGCTATAAAGGTTGTGGCTTTGGCATTGGTCTTGATGGTTGTGATTGGTTTTGGTGAAGCTCAAACCATTTGCAACATGCCAGTTGCTGGTTTGTATGCATGTAGACCGTCGGTGACACCTCCGAACCCGACACCCCCGACCGCTCAATGTTGCATGGCGCTCTCGCACGCCGACTTGCACTGCTTTTGCACTTACCGGAACTCGGGAGCTCTTTCTTCTCTTGGCATTGACCCTAATCTTGCAATGCAGTTACCTAAGATCTGCAAGCTTCCTAACTCTCCAAATTGCTAG

Protein sequence

MDKAIKVVALALVLMVVIGFGEAQTICNMPVAGLYACRPSVTPPNPTPPTAQCCMALSHADLHCFCTYRNSGALSSLGIDPNLAMQLPKICKLPNSPNC
BLAST of Lsi07G000860 vs. Swiss-Prot
Match: DIRL1_ARATH (Putative lipid-transfer protein DIR1 OS=Arabidopsis thaliana GN=DIR1 PE=1 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 6.1e-16
Identity = 40/96 (41.67%), Postives = 58/96 (60.42%), Query Frame = 1

Query: 4   AIKVVALALVLMVVIGFGEAQTICNMPVAGLYACRPSVTPPNPTPPTAQCCMALSHADLH 63
           A+ ++A+ +++ +++    A  +C M    L  C+P+V+  NPT P+  CC AL HAD  
Sbjct: 7   AMVMMAMIVIMAMLVDTSVAIDLCGMSQDELNECKPAVSKENPTSPSQPCCTALQHADFA 66

Query: 64  CFCTYRNSGALSSLGIDPNLAMQLPKICKLPNSPNC 100
           C C Y+NS  L S G+DP LA  LPK C L N+P C
Sbjct: 67  CLCGYKNSPWLGSFGVDPELASALPKQCGLANAPTC 102

BLAST of Lsi07G000860 vs. TrEMBL
Match: A0A0A0KZ41_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G017140 PE=4 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 2.5e-40
Identity = 83/101 (82.18%), Postives = 90/101 (89.11%), Query Frame = 1

Query: 1   MDKAIKVVALALVLMVV--IGFGEAQTICNMPVAGLYACRPSVTPPNPTPPTAQCCMALS 60
           MDKA+KVVALALVLMVV  IGFGEAQ+ICNMP+AGLYACRPSVTPPNPTPPT QCC AL+
Sbjct: 1   MDKAMKVVALALVLMVVNNIGFGEAQSICNMPIAGLYACRPSVTPPNPTPPTTQCCSALT 60

Query: 61  HADLHCFCTYRNSGALSSLGIDPNLAMQLPKICKLPNSPNC 100
           HADLHCFC YRNSGALSS GI+P LAM+LPK C +  SPNC
Sbjct: 61  HADLHCFCAYRNSGALSSFGINPELAMELPKRCNISKSPNC 101

BLAST of Lsi07G000860 vs. TrEMBL
Match: A0A0A1IXE5_CUCSA (Lipid binding/ Lipid transporter protein OS=Cucumis sativus GN=LBP PE=2 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 7.2e-40
Identity = 82/101 (81.19%), Postives = 90/101 (89.11%), Query Frame = 1

Query: 1   MDKAIKVVALALVLMVV--IGFGEAQTICNMPVAGLYACRPSVTPPNPTPPTAQCCMALS 60
           MDKA+KVVALALVLMVV  IGFG+AQ+ICNMP+AGLYACRPSVTPPNPTPPT QCC AL+
Sbjct: 1   MDKAMKVVALALVLMVVNNIGFGKAQSICNMPIAGLYACRPSVTPPNPTPPTTQCCSALT 60

Query: 61  HADLHCFCTYRNSGALSSLGIDPNLAMQLPKICKLPNSPNC 100
           HADLHCFC YRNSGALSS GI+P LAM+LPK C +  SPNC
Sbjct: 61  HADLHCFCAYRNSGALSSFGINPELAMELPKRCNISKSPNC 101

BLAST of Lsi07G000860 vs. TrEMBL
Match: A0A0D2VM18_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_011G140600 PE=4 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 2.8e-28
Identity = 58/97 (59.79%), Postives = 77/97 (79.38%), Query Frame = 1

Query: 3   KAIKVVALALVLMVVIGFGEAQTICNMPVAGLYACRPSVTPPNPTPPTAQCCMALSHADL 62
           K ++ + LA+V+++ I   +AQ+ICNMP +GL AC+P+VTPPNP PPT+ CC ALSHAD+
Sbjct: 5   KGLQALILAMVMVMAIATTDAQSICNMPASGLMACKPAVTPPNPPPPTSTCCSALSHADM 64

Query: 63  HCFCTYRNSGALSSLGIDPNLAMQLPKICKLPNSPNC 100
            C C+Y+NS  L SLGIDPNLAM+LP +CKLP+  NC
Sbjct: 65  RCLCSYKNSKLLPSLGIDPNLAMKLPSLCKLPHPANC 101

BLAST of Lsi07G000860 vs. TrEMBL
Match: A0A0D2USW2_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_011G140500 PE=4 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 1.8e-27
Identity = 56/97 (57.73%), Postives = 77/97 (79.38%), Query Frame = 1

Query: 3   KAIKVVALALVLMVVIGFGEAQTICNMPVAGLYACRPSVTPPNPTPPTAQCCMALSHADL 62
           K ++ + LA+++++ I   +AQ+ICNMP +GL AC+P+VTPPNP PPT+ CC ALS+AD+
Sbjct: 5   KGLQALILAMIMVMAIATTDAQSICNMPASGLMACKPAVTPPNPPPPTSTCCSALSYADM 64

Query: 63  HCFCTYRNSGALSSLGIDPNLAMQLPKICKLPNSPNC 100
            C C+Y+NS  L SLGIDPNLAM+LP +CKLP+  NC
Sbjct: 65  RCLCSYKNSKLLPSLGIDPNLAMKLPSLCKLPHPANC 101

BLAST of Lsi07G000860 vs. TrEMBL
Match: D7U9P1_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_05s0062g01260 PE=4 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 3.5e-26
Identity = 56/98 (57.14%), Postives = 73/98 (74.49%), Query Frame = 1

Query: 2   DKAIKVVALALVLMVVIGFGEAQTICNMPVAGLYACRPSVTPPNPTPPTAQCCMALSHAD 61
           +KA   +A+  +++  +    AQTICNM   GL AC+P+V+PPNP PP+A CC ALSHAD
Sbjct: 17  NKAFGKLAIVALVLASLASSNAQTICNMSGEGLMACKPAVSPPNPAPPSAACCSALSHAD 76

Query: 62  LHCFCTYRNSGALSSLGIDPNLAMQLPKICKLPNSPNC 100
           +HC C+YRNS  L S+GIDPNLA+QLPK CK PN+ +C
Sbjct: 77  MHCLCSYRNSKLLPSMGIDPNLALQLPKKCKFPNAAHC 114

BLAST of Lsi07G000860 vs. TAIR10
Match: AT5G48485.1 (AT5G48485.1 Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein)

HSP 1 Score: 84.7 bits (208), Expect = 3.4e-17
Identity = 40/96 (41.67%), Postives = 58/96 (60.42%), Query Frame = 1

Query: 4   AIKVVALALVLMVVIGFGEAQTICNMPVAGLYACRPSVTPPNPTPPTAQCCMALSHADLH 63
           A+ ++A+ +++ +++    A  +C M    L  C+P+V+  NPT P+  CC AL HAD  
Sbjct: 7   AMVMMAMIVIMAMLVDTSVAIDLCGMSQDELNECKPAVSKENPTSPSQPCCTALQHADFA 66

Query: 64  CFCTYRNSGALSSLGIDPNLAMQLPKICKLPNSPNC 100
           C C Y+NS  L S G+DP LA  LPK C L N+P C
Sbjct: 67  CLCGYKNSPWLGSFGVDPELASALPKQCGLANAPTC 102

BLAST of Lsi07G000860 vs. TAIR10
Match: AT5G48490.1 (AT5G48490.1 Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein)

HSP 1 Score: 84.0 bits (206), Expect = 5.8e-17
Identity = 45/98 (45.92%), Postives = 57/98 (58.16%), Query Frame = 1

Query: 6   KVVALALVLMV----VIGFGEAQTICNMPVAGLYACRPSVTPPNPTPPTAQCCMALSHAD 65
           K VA+ +++MV    V+    A  +C M  A L  C P+V+  NPT P+  CC AL HAD
Sbjct: 4   KKVAIMVIVMVMASLVVERSVAIDLCGMTQAELNECLPAVSKNNPTSPSLLCCNALKHAD 63

Query: 66  LHCFCTYRNSGALSSLGIDPNLAMQLPKICKLPNSPNC 100
             C C Y+NS  L S G+DP LA  LPK C L N+P C
Sbjct: 64  YTCLCGYKNSPWLGSFGVDPKLASSLPKECDLTNAPTC 101

BLAST of Lsi07G000860 vs. TAIR10
Match: AT5G55450.1 (AT5G55450.1 Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein)

HSP 1 Score: 60.8 bits (146), Expect = 5.3e-10
Identity = 39/93 (41.94%), Postives = 50/93 (53.76%), Query Frame = 1

Query: 9   ALALVLMVVIGFGEAQTI--CNMPVAGLYACRPSVTPPNPTPPTAQCCMALSHADLHCFC 68
           ALA+VL   I   EA +I  CN+    L  CRP+VT  NP PP   CC     A+L C C
Sbjct: 14  ALAMVLTAAIMVKEATSIPVCNIDTNDLAKCRPAVTGNNPPPPGPDCCAVARVANLQCLC 73

Query: 69  TYRNSGALSSLGIDPNLAMQLPKICKLPNSPNC 100
            Y+    L ++GIDP+    L   C + NSP+C
Sbjct: 74  PYK--PYLPTVGIDPSRVRPLLANCGV-NSPSC 103

BLAST of Lsi07G000860 vs. TAIR10
Match: AT5G55410.2 (AT5G55410.2 Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein)

HSP 1 Score: 51.6 bits (122), Expect = 3.2e-07
Identity = 26/89 (29.21%), Postives = 45/89 (50.56%), Query Frame = 1

Query: 9   ALALVLMVVIGFGEAQTICNMPVAGLYACRPSVTPPNPTPPTAQCCMALSHADLHCFCTY 68
           A+ L   +++    + +IC+M +  +  CRP++T  NP PP   CC+ +  A+  C C +
Sbjct: 16  AMVLTFAMMVKEATSMSICDMDINDMQKCRPAITGNNPPPPVNDCCVVVRKANFECLCRF 75

Query: 69  RNSGALSSLGIDPNLAMQLPKICKLPNSP 98
           +    L  L IDP+  + L   C +   P
Sbjct: 76  K--FYLPILRIDPSKVVALVAKCGVTTVP 102

BLAST of Lsi07G000860 vs. NCBI nr
Match: gi|700197968|gb|KGN53126.1| (hypothetical protein Csa_4G017140 [Cucumis sativus])

HSP 1 Score: 172.6 bits (436), Expect = 3.5e-40
Identity = 83/101 (82.18%), Postives = 90/101 (89.11%), Query Frame = 1

Query: 1   MDKAIKVVALALVLMVV--IGFGEAQTICNMPVAGLYACRPSVTPPNPTPPTAQCCMALS 60
           MDKA+KVVALALVLMVV  IGFGEAQ+ICNMP+AGLYACRPSVTPPNPTPPT QCC AL+
Sbjct: 1   MDKAMKVVALALVLMVVNNIGFGEAQSICNMPIAGLYACRPSVTPPNPTPPTTQCCSALT 60

Query: 61  HADLHCFCTYRNSGALSSLGIDPNLAMQLPKICKLPNSPNC 100
           HADLHCFC YRNSGALSS GI+P LAM+LPK C +  SPNC
Sbjct: 61  HADLHCFCAYRNSGALSSFGINPELAMELPKRCNISKSPNC 101

BLAST of Lsi07G000860 vs. NCBI nr
Match: gi|659107927|ref|XP_008453924.1| (PREDICTED: putative lipid-transfer protein DIR1 [Cucumis melo])

HSP 1 Score: 172.6 bits (436), Expect = 3.5e-40
Identity = 84/101 (83.17%), Postives = 89/101 (88.12%), Query Frame = 1

Query: 1   MDKAIKVVALALVLMVV--IGFGEAQTICNMPVAGLYACRPSVTPPNPTPPTAQCCMALS 60
           MDKA+KVV LALVLMVV  IGFGEAQ+ICNMP+AGLYACRP+VTPPNPT PTAQCCMAL 
Sbjct: 1   MDKAMKVVVLALVLMVVNNIGFGEAQSICNMPIAGLYACRPAVTPPNPTAPTAQCCMALG 60

Query: 61  HADLHCFCTYRNSGALSSLGIDPNLAMQLPKICKLPNSPNC 100
            ADLHCFC YRNSGALSS GIDPNLAMQLPK C +  SPNC
Sbjct: 61  QADLHCFCAYRNSGALSSFGIDPNLAMQLPKRCNISKSPNC 101

BLAST of Lsi07G000860 vs. NCBI nr
Match: gi|821595415|ref|NP_001295798.1| (lipopolysaccharide-binding protein precursor [Cucumis sativus])

HSP 1 Score: 171.0 bits (432), Expect = 1.0e-39
Identity = 82/101 (81.19%), Postives = 90/101 (89.11%), Query Frame = 1

Query: 1   MDKAIKVVALALVLMVV--IGFGEAQTICNMPVAGLYACRPSVTPPNPTPPTAQCCMALS 60
           MDKA+KVVALALVLMVV  IGFG+AQ+ICNMP+AGLYACRPSVTPPNPTPPT QCC AL+
Sbjct: 1   MDKAMKVVALALVLMVVNNIGFGKAQSICNMPIAGLYACRPSVTPPNPTPPTTQCCSALT 60

Query: 61  HADLHCFCTYRNSGALSSLGIDPNLAMQLPKICKLPNSPNC 100
           HADLHCFC YRNSGALSS GI+P LAM+LPK C +  SPNC
Sbjct: 61  HADLHCFCAYRNSGALSSFGINPELAMELPKRCNISKSPNC 101

BLAST of Lsi07G000860 vs. NCBI nr
Match: gi|823250126|ref|XP_012457661.1| (PREDICTED: putative lipid-transfer protein DIR1 [Gossypium raimondii])

HSP 1 Score: 132.5 bits (332), Expect = 4.1e-28
Identity = 58/97 (59.79%), Postives = 77/97 (79.38%), Query Frame = 1

Query: 3   KAIKVVALALVLMVVIGFGEAQTICNMPVAGLYACRPSVTPPNPTPPTAQCCMALSHADL 62
           K ++ + LA+V+++ I   +AQ+ICNMP +GL AC+P+VTPPNP PPT+ CC ALSHAD+
Sbjct: 5   KGLQALILAMVMVMAIATTDAQSICNMPASGLMACKPAVTPPNPPPPTSTCCSALSHADM 64

Query: 63  HCFCTYRNSGALSSLGIDPNLAMQLPKICKLPNSPNC 100
            C C+Y+NS  L SLGIDPNLAM+LP +CKLP+  NC
Sbjct: 65  RCLCSYKNSKLLPSLGIDPNLAMKLPSLCKLPHPANC 101

BLAST of Lsi07G000860 vs. NCBI nr
Match: gi|823242124|ref|XP_012453760.1| (PREDICTED: putative lipid-transfer protein DIR1 [Gossypium raimondii])

HSP 1 Score: 129.8 bits (325), Expect = 2.6e-27
Identity = 56/97 (57.73%), Postives = 77/97 (79.38%), Query Frame = 1

Query: 3   KAIKVVALALVLMVVIGFGEAQTICNMPVAGLYACRPSVTPPNPTPPTAQCCMALSHADL 62
           K ++ + LA+++++ I   +AQ+ICNMP +GL AC+P+VTPPNP PPT+ CC ALS+AD+
Sbjct: 5   KGLQALILAMIMVMAIATTDAQSICNMPASGLMACKPAVTPPNPPPPTSTCCSALSYADM 64

Query: 63  HCFCTYRNSGALSSLGIDPNLAMQLPKICKLPNSPNC 100
            C C+Y+NS  L SLGIDPNLAM+LP +CKLP+  NC
Sbjct: 65  RCLCSYKNSKLLPSLGIDPNLAMKLPSLCKLPHPANC 101

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
DIRL1_ARATH6.1e-1641.67Putative lipid-transfer protein DIR1 OS=Arabidopsis thaliana GN=DIR1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KZ41_CUCSA2.5e-4082.18Uncharacterized protein OS=Cucumis sativus GN=Csa_4G017140 PE=4 SV=1[more]
A0A0A1IXE5_CUCSA7.2e-4081.19Lipid binding/ Lipid transporter protein OS=Cucumis sativus GN=LBP PE=2 SV=1[more]
A0A0D2VM18_GOSRA2.8e-2859.79Uncharacterized protein OS=Gossypium raimondii GN=B456_011G140600 PE=4 SV=1[more]
A0A0D2USW2_GOSRA1.8e-2757.73Uncharacterized protein OS=Gossypium raimondii GN=B456_011G140500 PE=4 SV=1[more]
D7U9P1_VITVI3.5e-2657.14Putative uncharacterized protein OS=Vitis vinifera GN=VIT_05s0062g01260 PE=4 SV=... [more]
Match NameE-valueIdentityDescription
AT5G48485.13.4e-1741.67 Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumi... [more]
AT5G48490.15.8e-1745.92 Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumi... [more]
AT5G55450.15.3e-1041.94 Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumi... [more]
AT5G55410.23.2e-0729.21 Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumi... [more]
Match NameE-valueIdentityDescription
gi|700197968|gb|KGN53126.1|3.5e-4082.18hypothetical protein Csa_4G017140 [Cucumis sativus][more]
gi|659107927|ref|XP_008453924.1|3.5e-4083.17PREDICTED: putative lipid-transfer protein DIR1 [Cucumis melo][more]
gi|821595415|ref|NP_001295798.1|1.0e-3981.19lipopolysaccharide-binding protein precursor [Cucumis sativus][more]
gi|823250126|ref|XP_012457661.1|4.1e-2859.79PREDICTED: putative lipid-transfer protein DIR1 [Gossypium raimondii][more]
gi|823242124|ref|XP_012453760.1|2.6e-2757.73PREDICTED: putative lipid-transfer protein DIR1 [Gossypium raimondii][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR016140Bifunc_inhib/LTP/seed_store
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
biological_process GO:0009627 systemic acquired resistance
cellular_component GO:0016020 membrane
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function
molecular_function GO:0005504 fatty acid binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi07G000860.1Lsi07G000860.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016140Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domainPFAMPF14368LTP_2coord: 22..99
score: 5.9
IPR016140Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domainSMARTSM00499aai_6coord: 27..99
score: 6.
IPR016140Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domainunknownSSF47699Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumincoord: 27..98
score: 8.04
NoneNo IPR availableGENE3DG3DSA:1.10.110.10coord: 33..95
score: 9.
NoneNo IPR availablePANTHERPTHR33122FAMILY NOT NAMEDcoord: 1..98
score: 2.5
NoneNo IPR availablePANTHERPTHR33122:SF5SUBFAMILY NOT NAMEDcoord: 1..98
score: 2.5