Lsi06G011220 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi06G011220
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionF-box protein PP2-B1
Locationchr06 : 21493558 .. 21496580 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTAATTTAGCATTTAAACCAAAACAAACTCCCCTGATTGCCTGTGTCCCCGATGCGAATGACGCAATGGAATAGGAAACGAGGATGGAAACAGAGGACGAAGAAATTGGCGATTTATCTTTTATGCCCGAAGGAGTAATAGCCAACCTCTTATCTCTCACAAGTCCAATCGATGCCTGCAGGTCGGCGGCGGTTTCCCCAATATTTAATGCGGCGGCGCAGTCGGATTTTGTATGGGACAGGTTCCTGCCTACCGATTGGGAAGTTTTGATTTCTCAGAGGAAATCTGATGATCACAATGTTGATCCAATTTCCACTTCGAAGAAGGAGATCTTTTTCTCCCTTTGCAACAGCCCGGTGCTAATTGACGACGGCAACAAGGTAAGCTTTCCGATTCCCCCGATTTCTTTAAATTGAAGGTGGCCGGAGTTGGATCAGTGGTCGGCAAACACAACTCTGACTCCACCCCTGCCATCATCGGAAGTTGCCTTCCGAATTGACCTTGGAGGGGCCCCATTTTATTCTTTATATATTCATTTTGTTAGTTTCATTTTGGCAGTGATTTTAAAATATTAAAATCAGCTTTGTCACTAAACCTTAATCACACGAAATTTAAATTCACACTTTTAAATGTATGTTTCATATTATTTTAATATATTTTGAATAATATATTACAAATTTGATCTCTATACTTTAGAGAAAATTAAAATGTAGTCCTTATAATTTATAATTAGAATTTGGTCCCATAAAACTTCCATAAATAGTTTCTACGGTAAGGACTATTCATGAAGATTTTATTACACCATATCGACTATTTATGAGATTTTATCAAACCATAGGACCAAATTCTAACTTTTAAAATCATAGTGACTAAATTCTAACTTTTTCCAAATCATGAGGACCAAATTTGTAAGTTAATTAAAATATTTTACGAATGAAAATGATAAAATTAATTTTAAACATTTTAGAATCATGCCTATGTTGTGAAAAAGGACTGTGACCAAATAGAGTAAAGTCGCGGCTAAAGTAGATTGAGAATAATAATAATGATGAAGAAGGAGGGAGAGAATAACGTATAGTGATTTACTTGGAAACCTCCTAAACAAACTAGGGTAAAAATCATGGACAAGGATAGAAGAATTCTAAACTATATATAACATAAGAGTTACAAACTTTCTTTCTTATATAGGAATAACAAGCTCTAAACTCTCTCTATTTTAAGTCTCATACTTTGCTCACTTGTTGGAGATTTTGGGATGATTTGTCAACCATGGGTCCCCTCCTATTTATAGCCCAAAGAAACTTGATCATCAAGCAAATCTAATGACTCCAAATTGTCCTTGATAGATGTGAAAGATCTAATGGTGATAAATTGGTCTTGGACATGATGTAAGATCCAATGACCCAAAATTGCTTCTAAGAAATTTATTTAATAATAAATAGGATAATCGTCATGAGTAGTAATTTTAGGGATAATAATCAAGTGTATAACAACATTTTAAAAAAAATTGTAAATATAACAAAGTCTATCAGTAATAGACCACCTAGACCATAAAAGTCTATCATCAATAAAAGTCTATAATTGATAGACTTTGCTATATTTGTAATTTTTTTAAAACGTTGCTATATATGCTAATAATTGCTATATTTGTAACGGTACTAATAAATAAGCTCTTGAACTAAGAGATAACATGCCATTAACATTGTTCTCAAGTATGGAGTTTAAAGTTTTATTAGAAAATATGAACTAAGACTCCGCTGACCCCTATTTCAAGTCTATAGATCATTGATTAAATATGTGCATCTTTTGTTGGATGAATGATGAAAATTTTATGCACTTTATTTGTATGGATATAAATTGAGTGGGGTTAAAATCATTGGTATGATACAGAGCTTGTCATTGGATAAATGGAGTGGTAGGAAATGCATAATGCTTGGAGCAAGAGATCTATCGGTCATTTGGGGTGATAAGTCTTTGTATTGGACCTGGTTTCACCATCCTCTTTCGAGGTTCAAAGTCTATGAGATTGAAGCAAATCACATTCTCTATCTTTTTGTTTTTTTTTTGTCTCTCCCAATTCTAATACTCTGTTTTTTCAATGTGGGTGTGTGAGAATCAGATTTGCAGAGGTAGCCGTACTCCTCAATGTATGGTGGCTTGAAATACGTGGAAGAATAAATTGCAAAATGTTGTCTCCTAAAACAACTTATGCAGTTTACTTTGTGTTCAGGATGAGGAACCATAATTACGTAGGGTTCGATGTTAATCTTGCTGATGCCACAGTGGGAATTATTGGCACTGAAAACCATCATAGAAGTGTTTGTTTGGACCCTCATTTGGACAACCCTCGACGGTGGCAACCACGACGGAATGGACCGTGGTTAATGAGGCCTCTGCCTGGACCGTCTCTAGACATGTTAGGGTTAGAATGGCCACAGGAGAGACGTGATGGATGGTTTGAAATTGAGTTGGGAGAGTTCGAAAGTGGTGATGGAGATGATGAAGTTGAGATGGCTGTTATGGAGGTGAAAGGCCATTCTGCCAAGAGTGGCCTTGTCGTCGAGGGGATTGAGATTAGGCCTAAACATAGCTGGAGTTTATTAAAGCCGTGGTATTGCAAAATAGCGAGCCACGATATAAGTAAAATTTCAATAAACTTCAATTGAATAATATTGTTACTATTTTTTTTTTTTACTCATGAACGCGGGCGAGAATGGAGATGAGGATGCGATCGTAGAATTTTAAAGCTTATGGATCATGAGGATACTATTGTGTACTCCCACAACTAGTTGGTGTTCGTGTGTCATGCATTCTAGAGTTTTGAAACTTGGTTGTGAGATTATATATCTATAGAGCAATGGTCAGGTGTAACTTGTTGTACCTAATTTCATACCCCTTTTTTAATACATAAAGAAAAAGTTCAAAAAAATAATTTTTTTAAAAAAAGAGTTGTCAAAAAGATGCTATTTGTAAGAGAGGAAAACTTGTTAAAGTTATTTATGATCATTTCTCATTCATTTTA

mRNA sequence

CCTAATTTAGCATTTAAACCAAAACAAACTCCCCTGATTGCCTGTGTCCCCGATGCGAATGACGCAATGGAATAGGAAACGAGGATGGAAACAGAGGACGAAGAAATTGGCGATTTATCTTTTATGCCCGAAGGAGTAATAGCCAACCTCTTATCTCTCACAAGTCCAATCGATGCCTGCAGGTCGGCGGCGGTTTCCCCAATATTTAATGCGGCGGCGCAGTCGGATTTTGTATGGGACAGGTTCCTGCCTACCGATTGGGAAGTTTTGATTTCTCAGAGGAAATCTGATGATCACAATGTTGATCCAATTTCCACTTCGAAGAAGGAGATCTTTTTCTCCCTTTGCAACAGCCCGGTGCTAATTGACGACGGCAACAAGAGCTTGTCATTGGATAAATGGAGTGGTAGGAAATGCATAATGCTTGGAGCAAGAGATCTATCGGTCATTTGGGGTGATAAGTCTTTGTATTGGACCTGGTTTCACCATCCTCTTTCGAGGTTCAAAGTCTATGAGATTGAAGCAAATCACATTCTCTATCTTTTTGTTTTTTTTTTGTCTCTCCCAATTCTAATACTCTGTTTTTTCAATGTGGGTGTATTTGCAGAGGTAGCCGTACTCCTCAATGTATGGTGGCTTGAAATACGTGGAAGAATAAATTGCAAAATGTTGTCTCCTAAAACAACTTATGCAGTTTACTTTGTGTTCAGGATGAGGAACCATAATTACGTAGGGTTCGATGTTAATCTTGCTGATGCCACAGTGGGAATTATTGGCACTGAAAACCATCATAGAAGTGTTTGTTTGGACCCTCATTTGGACAACCCTCGACGGTGGCAACCACGACGGAATGGACCGTGGTTAATGAGGCCTCTGCCTGGACCGTCTCTAGACATGTTAGGGTTAGAATGGCCACAGGAGAGACGTGATGGATGGTTTGAAATTGAGTTGGGAGAGTTCGAAAGTGGTGATGGAGATGATGAAGTTGAGATGGCTGTTATGGAGGTGAAAGGCCATTCTGCCAAGAGTGGCCTTGTCGTCGAGGGGATTGAGATTAGGCCTAAACATAGCTGGAGTTTATTAAAGCCGTGGTATTGCAAAATAGCGAGCCACGATATAAGTAAAATTTCAATAAACTTCAATTGAATAATATTGTTACTATTTTTTTTTTTTACTCATGAACGCGGGCGAGAATGGAGATGAGGATGCGATCGTAGAATTTTAAAGCTTATGGATCATGAGGATACTATTGTGTACTCCCACAACTAGTTGGTGTTCGTGTGTCATGCATTCTAGAGTTTTGAAACTTGGTTGTGAGATTATATATCTATAGAGCAATGGTCAGGTGTAACTTGTTGTACCTAATTTCATACCCCTTTTTTAATACATAAAGAAAAAGTTCAAAAAAATAATTTTTTTAAAAAAAGAGTTGTCAAAAAGATGCTATTTGTAAGAGAGGAAAACTTGTTAAAGTTATTTATGATCATTTCTCATTCATTTTA

Coding sequence (CDS)

ATGGAAACAGAGGACGAAGAAATTGGCGATTTATCTTTTATGCCCGAAGGAGTAATAGCCAACCTCTTATCTCTCACAAGTCCAATCGATGCCTGCAGGTCGGCGGCGGTTTCCCCAATATTTAATGCGGCGGCGCAGTCGGATTTTGTATGGGACAGGTTCCTGCCTACCGATTGGGAAGTTTTGATTTCTCAGAGGAAATCTGATGATCACAATGTTGATCCAATTTCCACTTCGAAGAAGGAGATCTTTTTCTCCCTTTGCAACAGCCCGGTGCTAATTGACGACGGCAACAAGAGCTTGTCATTGGATAAATGGAGTGGTAGGAAATGCATAATGCTTGGAGCAAGAGATCTATCGGTCATTTGGGGTGATAAGTCTTTGTATTGGACCTGGTTTCACCATCCTCTTTCGAGGTTCAAAGTCTATGAGATTGAAGCAAATCACATTCTCTATCTTTTTGTTTTTTTTTTGTCTCTCCCAATTCTAATACTCTGTTTTTTCAATGTGGGTGTATTTGCAGAGGTAGCCGTACTCCTCAATGTATGGTGGCTTGAAATACGTGGAAGAATAAATTGCAAAATGTTGTCTCCTAAAACAACTTATGCAGTTTACTTTGTGTTCAGGATGAGGAACCATAATTACGTAGGGTTCGATGTTAATCTTGCTGATGCCACAGTGGGAATTATTGGCACTGAAAACCATCATAGAAGTGTTTGTTTGGACCCTCATTTGGACAACCCTCGACGGTGGCAACCACGACGGAATGGACCGTGGTTAATGAGGCCTCTGCCTGGACCGTCTCTAGACATGTTAGGGTTAGAATGGCCACAGGAGAGACGTGATGGATGGTTTGAAATTGAGTTGGGAGAGTTCGAAAGTGGTGATGGAGATGATGAAGTTGAGATGGCTGTTATGGAGGTGAAAGGCCATTCTGCCAAGAGTGGCCTTGTCGTCGAGGGGATTGAGATTAGGCCTAAACATAGCTGGAGTTTATTAAAGCCGTGGTATTGCAAAATAGCGAGCCACGATATAAGTAAAATTTCAATAAACTTCAATTGA

Protein sequence

METEDEEIGDLSFMPEGVIANLLSLTSPIDACRSAAVSPIFNAAAQSDFVWDRFLPTDWEVLISQRKSDDHNVDPISTSKKEIFFSLCNSPVLIDDGNKSLSLDKWSGRKCIMLGARDLSVIWGDKSLYWTWFHHPLSRFKVYEIEANHILYLFVFFLSLPILILCFFNVGVFAEVAVLLNVWWLEIRGRINCKMLSPKTTYAVYFVFRMRNHNYVGFDVNLADATVGIIGTENHHRSVCLDPHLDNPRRWQPRRNGPWLMRPLPGPSLDMLGLEWPQERRDGWFEIELGEFESGDGDDEVEMAVMEVKGHSAKSGLVVEGIEIRPKHSWSLLKPWYCKIASHDISKISINFN
BLAST of Lsi06G011220 vs. Swiss-Prot
Match: P2B10_ARATH (F-box protein PP2-B10 OS=Arabidopsis thaliana GN=PP2B10 PE=1 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 7.9e-50
Identity = 116/315 (36.83%), Postives = 167/315 (53.02%), Query Frame = 1

Query: 15  PEGVIANLLSLTSPIDACRSAAVSPIFNAAAQSDFVWDRFLPTDWEVLISQRKSDDHNVD 74
           PE  I+ ++S T+P DAC +A VS  F +  +SD +W++FLP D+E LI   +       
Sbjct: 18  PEDCISYIISFTNPRDACVAATVSKTFESTVKSDIIWEKFLPADYESLIPPSR------- 77

Query: 75  PISTSKKEIFFSLCNSPVLIDDGNKSLSLDKWSGRKCIMLGARDLSVIWGDKSLYWTWFH 134
            + +SKKE++FSLCN PVL DD  KS+ L+K SG++C+ML A +LS+IWGD   YW    
Sbjct: 78  -VFSSKKELYFSLCNDPVLFDDDKKSVWLEKASGKRCLMLSAMNLSIIWGDNPQYW---- 137

Query: 135 HPLSRFKVYEIEANHILYLFVFFLSLPILILCFFNVGVFAEVAVLLNVWWLEIRGRINCK 194
                                 ++ +P           F +VA L +V W EIRGR N +
Sbjct: 138 ---------------------QWIPIP--------ESRFEKVAKLRDVCWFEIRGRTNTR 197

Query: 195 MLSPKTTYAVYFVFRMRNHNYVGFDVNLADATVGIIGTENHHRSVCLDPHLDNPRRWQPR 254
           +LSP+T Y+ Y VF+  +  Y GF     +A VG++G E   R +C    +   RR    
Sbjct: 198 VLSPRTRYSAYIVFKGVDKCY-GFQNVAIEAAVGVVGQEPSRRLICFSEAIRRGRR---- 257

Query: 255 RNGPWLMRPLPGPSLDMLGLEWPQERRDGWFEIELGEFESGDG---DDEVEMAVMEVKGH 314
                              +  P++R DGW EIELGEF +  G   +DE+EM+ +E K  
Sbjct: 258 ------------------NVVKPKQREDGWMEIELGEFFNDGGIMDNDEIEMSALETKQL 268

Query: 315 SAKSGLVVEGIEIRP 327
           + K GL+++GIEIRP
Sbjct: 318 NRKCGLIIQGIEIRP 268

BLAST of Lsi06G011220 vs. Swiss-Prot
Match: FB95_ARATH (F-box protein At2g02240 OS=Arabidopsis thaliana GN=At2g02240 PE=2 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 5.7e-48
Identity = 114/317 (35.96%), Postives = 173/317 (54.57%), Query Frame = 1

Query: 14  MPEGVIANLLSLTSPIDACRSAAVSPIFNAAAQSDFVWDRFLPTDWEVLISQRKSDDHNV 73
           +PE  I+N++S TSP DAC +A+VS  F +A  SD VWD+FLP ++E L+S+ +      
Sbjct: 64  LPEDCISNIISFTSPRDACVAASVSKTFESAVSSDCVWDKFLPPEYESLVSRSR------ 123

Query: 74  DPISTSKKEIFFSLCNSPVLIDDGNKSLSLDKWSGRKCIMLGARDLSVIWGDKSLYWTWF 133
             +  SKKE++F+LC++PVLI+DG KS  L+K SG++CIML +++L + WG    YW W 
Sbjct: 124 --VFASKKELYFALCHNPVLIEDGKKSFWLEKASGKRCIMLSSKELWITWGSSPEYWQWI 183

Query: 134 HHPLSRF-KVYEIEANHILYLFVFFLSLPILILCFFNVGVFAEVAVLLNVWWLEIRGRIN 193
             P SRF K+ E                 +L +C+F                 EIRG+ +
Sbjct: 184 SIPESRFNKIAE-----------------LLDVCWF-----------------EIRGKTS 243

Query: 194 CKMLSPKTTYAVYFVFRMRNHNYVGFDVNLADATVGIIGTENHHRSVCLDPHLDNPRRWQ 253
            ++LSP T Y+ Y VF+ ++    G      +  +G++G E+  R +    +   PR  +
Sbjct: 244 ARVLSPGTRYSAYIVFKTKD-RCPGLGHLPVEVGLGLVGQESSKRFI----YFIGPRDRR 303

Query: 254 PRRNGPWLMRPLPGPSLDMLGLEWPQERRDGWFEIELGEFESGDGDDEVEMAVMEVKGHS 313
            RR              +   +  P +R DGW E ELGEF + +  DE+E +V+E+K  S
Sbjct: 304 GRR--------------ETRDVTKPDQREDGWMEAELGEFFNEERCDEIEFSVIEIKSPS 319

Query: 314 AKSGLVVEGIEIRPKHS 330
            KSGL+++GIE RP  S
Sbjct: 364 WKSGLIIQGIEFRPTKS 319

BLAST of Lsi06G011220 vs. Swiss-Prot
Match: PP2B8_ARATH (Putative F-box protein PP2-B8 OS=Arabidopsis thaliana GN=PP2B8 PE=3 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 1.2e-45
Identity = 114/325 (35.08%), Postives = 176/325 (54.15%), Query Frame = 1

Query: 8   IGDLSFMPEGVIANLLSLTSPIDACRSAAVSPIFNAAAQSDFVWDRFLPTDWEVLISQRK 67
           + +L  +PE  ++ ++S TSP DAC  A+VS  F +A +SD VW++F+P ++E LISQ +
Sbjct: 33  VAELDDLPEECVSIIVSFTSPQDACVLASVSKTFASAVKSDIVWEKFIPPEYESLISQSR 92

Query: 68  SDDHNVDPISTSKKEIFFSLCNSPVLIDDGNKSLSLDKWSGRKCIMLGARDLSVIWGDKS 127
           +          SKKE++F+LC+  VLIDDG KSL ++K + ++CIM+ A +L++      
Sbjct: 93  AFKF------LSKKELYFALCDKSVLIDDGKKSLWIEKANAKRCIMISAMNLAI------ 152

Query: 128 LYWTWFHHPLSRFKVYEIEANHILYLFVFFLSLPILILCFFNVGVFAEVAVLLNVWWLEI 187
               W + P S   + + +A                         F  VA LL V   EI
Sbjct: 153 ---AWGNSPQSWRWIPDPQAR------------------------FETVAELLEVCLFEI 212

Query: 188 RGRINCKMLSPKTTYAVYFVFRMRNHNYVGFDVNLADATVGIIG---TENHHRSVCLDPH 247
           RGRIN +++SPKT Y+ Y V++  N  Y GF+    +  VG++G    E+  R +C D  
Sbjct: 213 RGRINSRVISPKTRYSAYIVYKKLNICY-GFENVAVEVVVGVVGQDLEESCRRYICFDET 272

Query: 248 LDNPRRWQPRRNGPWLMRPLPGPSLDMLGLEWPQERRDGWFEIELGEFESGDG---DDEV 307
           +D   +++ R  G  L++              P+ R+DGW EI++GEF +  G   DDE+
Sbjct: 273 MD--EQFRRRDRGKNLVK--------------PERRKDGWMEIKIGEFFNEGGLLNDDEI 301

Query: 308 EMAVMEVKGHSAKSGLVVEGIEIRP 327
           EM  +E K    K GL+++GIEIRP
Sbjct: 333 EMVALEAKQRHWKRGLIIQGIEIRP 301

BLAST of Lsi06G011220 vs. Swiss-Prot
Match: PP2B1_ARATH (F-box protein PP2-B1 OS=Arabidopsis thaliana GN=PP2B1 PE=1 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 4.5e-45
Identity = 119/342 (34.80%), Postives = 177/342 (51.75%), Query Frame = 1

Query: 14  MPEGVIANLLSLTSPIDACRSAAVSPIFNAAAQSDFVWDRFLPTDWEVLISQRKSDDHNV 73
           +PE  I+ ++S TSP DAC  A+VS    +AAQSD VW+ FLP+++  L+ Q  S +H  
Sbjct: 35  LPEDCISKVISHTSPRDACVVASVSKSVKSAAQSDLVWEMFLPSEYSSLVLQ--SANH-- 94

Query: 74  DPISTSKKEIFFSLCNSPVLIDDGNKSLSLDKWSGRKCIMLGARDLSVIWGDKSLYWTWF 133
                SKKEIF SL ++ VL+++G KS  ++K SG+KC ML A +L++IWGD        
Sbjct: 95  ----LSKKEIFLSLADNSVLVENGKKSFWVEKASGKKCYMLSAMELTIIWGDSP------ 154

Query: 134 HHPLSRFKVYEIEANHILYLFVFFLSLPILILCFFNVGVFAEVAVLLNVWWLEIRGRINC 193
                               +  ++++P           F +VA L NV W E+RG+I+C
Sbjct: 155 -------------------AYWKWITVP--------ESKFEKVAELRNVCWFEVRGKISC 214

Query: 194 KMLSPKTTYAVYFVFRMRNHNYVGFDVNLADATVGIIGTENHHRSVCLDPHLDNPRRWQP 253
            MLS  T Y+VY VF+  N    GFD+   +A VG +G     +SV  +    + R    
Sbjct: 215 GMLSKGTHYSVYVVFKTANGRSYGFDLVPVEAGVGFVGKVATKKSVYFESGNADSRSATS 274

Query: 254 RRNG--------------PWLM------RPLPGPSLDMLGLEWPQERRDGWFEIELGEFE 313
             +G              PW+         + G     + +  P+ER DGW E+ELG+F 
Sbjct: 275 HYSGISYAMVSRAFRMRRPWMQVQREEEEEVEGERERGMNVVGPKERVDGWSEVELGKFY 334

Query: 314 ------SGDGDDEVEMAVMEVKGHSAKSGLVVEGIEIRPKHS 330
                   DG DE+E+++ME +  + KSGL+++GIEIRP+ S
Sbjct: 335 INNGGCGDDGSDEIEISIMETQNGNWKSGLIIQGIEIRPERS 335

BLAST of Lsi06G011220 vs. Swiss-Prot
Match: SKIP3_ARATH (F-box protein SKIP3 OS=Arabidopsis thaliana GN=SKIP3 PE=1 SV=2)

HSP 1 Score: 182.2 bits (461), Expect = 1.0e-44
Identity = 119/320 (37.19%), Postives = 168/320 (52.50%), Query Frame = 1

Query: 11  LSFMPEGVIANLLSLTSPIDACRSAAVSPIFNAAAQSDFVWDRFLPTDWEVLISQRKSDD 70
           L  +PEG I+N++S TSP DAC +AAVS IF +A +SD VW++FLPTD+E LI+  +   
Sbjct: 24  LDSLPEGCISNIISFTSPEDACVAAAVSKIFESAVKSDIVWEKFLPTDYESLITPSR--- 83

Query: 71  HNVDPISTSKKEIFFSLCNSPVLIDDGNKSLSLDKWSGRKCIMLGARDLSV-IWGDKSLY 130
                + +SKKE++FSLCN P+LI+DG  SL L+K SG++CIML A  +++    D S  
Sbjct: 84  -----VFSSKKELYFSLCNDPLLIEDGKMSLWLEKASGKRCIMLSATAMNLSSMADMSQR 143

Query: 131 WTWFHHPLSRFKVYEIEANHILYLFVFFLSLPILILCFFNVGVFAEVAVLLNVWWLEIRG 190
           + W   P SRF+   + A    Y F                               E   
Sbjct: 144 FLWIPCPESRFET--VAALREAYRF-------------------------------EFNC 203

Query: 191 RINCKMLSPKTTYAVYFVFRMRNHNYVGFDVNLADATVGIIGTENHHRSVCLDPHLDNPR 250
           R+N ++LS +T Y+VY VF+ +  N+ GF     +A VGI+G E+    +C D H     
Sbjct: 204 RMNTRVLSLRTRYSVYIVFK-KADNWCGFKGVSIEAVVGIVGEESFRSFICFDTH----G 263

Query: 251 RWQPRRNGPWLMRPLPGPSLDMLGLEWPQERRDGWFEIELGEFESGDG---DDEVEMAVM 310
           + Q R+                  +  P+ R DGW E E+GEF +  G    DEVE++ +
Sbjct: 264 KGQARKRKV---------------VAKPELREDGWMETEIGEFYNEGGLMSSDEVEISTV 282

Query: 311 EVKGHSAKSGLVVEGIEIRP 327
           E K    K GLV+ GIEIRP
Sbjct: 324 EGKYAQQKRGLVILGIEIRP 282

BLAST of Lsi06G011220 vs. TrEMBL
Match: A0A0A0LUI2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G421930 PE=4 SV=1)

HSP 1 Score: 443.4 bits (1139), Expect = 2.7e-121
Identity = 223/340 (65.59%), Postives = 262/340 (77.06%), Query Frame = 1

Query: 4   EDEEIGDLSFMPEGVIANLLSLTSPIDACRSAAVSPIFNAAAQSDFVWDRFLPTDWEVLI 63
           ED+EIGDLSF+PEGVIAN+LS T+PIDAC +AAVS +FNAAAQSDFVWDRFLP DW+VLI
Sbjct: 17  EDQEIGDLSFLPEGVIANVLSFTTPIDACTAAAVSRVFNAAAQSDFVWDRFLPPDWDVLI 76

Query: 64  SQRKSDDHNVDPISTSKKEIFFSLCNSPVLIDDGNKSLSLDKWSGRKCIMLGARDLSVIW 123
           S RKS     DP+S+SKK+IFFSLCN+PVLIDD NKSLSLD+ SG+KCIMLGAR LS++W
Sbjct: 77  SYRKS----FDPLSSSKKDIFFSLCNNPVLIDDRNKSLSLDRQSGKKCIMLGARGLSLLW 136

Query: 124 GDKSLYWTWFHHPLSRFKVYEIEANHILYLFVFFLSLPILILCFFNVGVFAEVAVLLNVW 183
           GD S YW+W  HP SRF      A   + L  ++L L                       
Sbjct: 137 GDTSRYWSWDRHPRSRF------AEVAVLLKAWWLEL----------------------- 196

Query: 184 WLEIRGRINCKMLSPKTTYAVYFVFRMRNHNYVGFDVNLADATVGIIGTENH-HRSVCLD 243
               RGRI+CK+LSP+TTYAVYFVF+M+N NY GF+   ADATVGI+GTENH  RSVCLD
Sbjct: 197 ----RGRISCKILSPRTTYAVYFVFKMKNCNYEGFNFYPADATVGIVGTENHGRRSVCLD 256

Query: 244 PHLDNPRRWQPRRNGPWLMRPLPGPSLDMLGLEWPQERRDGWFEIELGEFESGDGDDEVE 303
           PHLDNPR+W   R G     P PGPS++MLGLEWPQERRDGWF+IELGEFESGDG DEVE
Sbjct: 257 PHLDNPRQW---RRG---FLPSPGPSVEMLGLEWPQERRDGWFQIELGEFESGDGADEVE 313

Query: 304 MAVMEVKGHSAKSGLVVEGIEIRPKHSWSLLKPWYCKIAS 343
           +A+MEVKGHS K+GL+VEG EIRPKHSW+LLKPW+C++A+
Sbjct: 317 IALMEVKGHSTKTGLIVEGFEIRPKHSWTLLKPWHCRVAT 313

BLAST of Lsi06G011220 vs. TrEMBL
Match: A0A0A0LX65_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G421920 PE=4 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 4.8e-86
Identity = 173/325 (53.23%), Postives = 215/325 (66.15%), Query Frame = 1

Query: 4   EDEEIGDLSFMPEGVIANLLSLTSPIDACRSAAVSPIFNAAAQSDFVWDRFLPTDWEVLI 63
           E E++ D S +PEGV+A +LSLT+P DAC S+AVS IF+AAAQSD VW++FLPTDWE+ I
Sbjct: 11  EGEQLSDFSSLPEGVVAKILSLTTPPDACVSSAVSKIFHAAAQSDIVWNQFLPTDWELFI 70

Query: 64  SQRKSDDHNVDPISTSKKEIFFSLCNSPVLIDDGNKSLSLDKWSGRKCIMLGARDLSVIW 123
           S+   +    DPI + KK+IFFSLC  PVLIDDGNKS SL+KWSG+KCIM+GARDLS+ W
Sbjct: 71  SRSNPNKLKFDPIFSPKKDIFFSLCYFPVLIDDGNKSFSLEKWSGKKCIMVGARDLSITW 130

Query: 124 GDKSLYWTWFHHPLSRFKVYEIEANHILYLFVFFLSLPILILCFFNVGVFAEVAVLLNVW 183
           G+ S   +W  HP SRF                     + +L F               W
Sbjct: 131 GENS-DSSWEDHPDSRF-------------------AEVAVLLFE--------------W 190

Query: 184 WLEIRGRINCKMLSPKTTYAVYFVFRMRNHNYVGFDVNLADATVGIIGTENHHRSVCLDP 243
           W EIRGR++C+MLSPKT YA YF+F++   +Y GF+++  +ATVGIIG ENH +SVCLDP
Sbjct: 191 WFEIRGRLSCRMLSPKTVYAAYFLFKLGERSYSGFNIDPTNATVGIIGHENHPKSVCLDP 250

Query: 244 HLDNPRRWQPRRNGPWLMRPLPGPSLDMLGLEWPQERRDGWFEIELGEFESGD-GDDEVE 303
           ++D P         P   +        M GLE P +R DGWFEIELGEF  GD GDDE+E
Sbjct: 251 YVDQPLYLDQLIQWPQWPQWRRQFERRMAGLERPHKRHDGWFEIELGEFIGGDHGDDELE 301

Query: 304 MAVMEVKGHSAKSGLVVEGIEIRPK 328
           MA+ EVKG S+K GLVVEGIEIRPK
Sbjct: 311 MALKEVKGSSSKGGLVVEGIEIRPK 301

BLAST of Lsi06G011220 vs. TrEMBL
Match: A0A0A0LU29_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G421900 PE=4 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 2.6e-84
Identity = 165/316 (52.22%), Postives = 213/316 (67.41%), Query Frame = 1

Query: 12  SFMPEGVIANLLSLTSPIDACRSAAVSPIFNAAAQSDFVWDRFLPTDWEVLISQRKSDDH 71
           S +PEGVIAN+LSLT+P+D CRS+AVS  F+AAAQSD VWD FLP D +  IS+ K    
Sbjct: 11  SSLPEGVIANILSLTTPLDVCRSSAVSRAFHAAAQSDIVWDTFLPNDLDDFISRSKPGGL 70

Query: 72  NVDPISTSKKEIFFSLCNSPVLIDDGNKSLSLDKWSGRKCIMLGARDLSVIWGDKSLYWT 131
           N DPI++SKKEIFFSLCNSP+LID+  KSLSLDK SG+KCIMLGARDLS++W D S+YWT
Sbjct: 71  NFDPITSSKKEIFFSLCNSPLLIDNAKKSLSLDKQSGKKCIMLGARDLSIVWDDTSVYWT 130

Query: 132 WFHHPLSRFKVYEIEANHILYLFVFFLSLPILILCFFNVGVFAEVAVLLNVWWLEIRGRI 191
           W  HP SRF         ++ +F                            WWLEIRG++
Sbjct: 131 WESHPESRF-------GEVVVIFK--------------------------AWWLEIRGKL 190

Query: 192 NCKMLSPKTTYAVYFVFRMRNHNYVGFDVNLADATVGIIGTENHHRSVCLDPHLDNPRRW 251
           +  MLSP+TTYA Y VF+MR   Y GF+++  DA VGI+G+E+  ++VCLDP+LD+P   
Sbjct: 191 SSGMLSPRTTYAAYIVFKMRERRYFGFNIDFVDAMVGIVGSEHSVKTVCLDPYLDDP--L 250

Query: 252 QPRRNGPWLMRPLPGPSLDMLGLEWPQERRDGWFEIELGEFESGDGDDEVEMAVMEVKGH 311
           Q RR+ P     LP  ++   GLE P+ R D WFE ELGEF++  GDDEVE+ + +++  
Sbjct: 251 QRRRHVPQAGSNLPTNNIS--GLEMPKGRHDDWFETELGEFDNNGGDDEVEIILKDLRCT 289

Query: 312 SAKSGLVVEGIEIRPK 328
            +K+ LVV+GIEIRPK
Sbjct: 311 DSKNSLVVQGIEIRPK 289

BLAST of Lsi06G011220 vs. TrEMBL
Match: A0A0A0LWS1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G421890 PE=4 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 1.4e-82
Identity = 175/333 (52.55%), Postives = 212/333 (63.66%), Query Frame = 1

Query: 2   ETEDEEIGDLSFMPEGVIANLLSLTSPIDACRSAAVSPIFNAAAQSDFVWDRFLPTDWEV 61
           E + E I D S +PEGV+A +LS T+P DACRS+ VS  F AAAQSD VWD FLPTDWE+
Sbjct: 4   EEKGEGISDFSSLPEGVVAKILSFTTPSDACRSSVVSTTFVAAAQSDIVWDSFLPTDWEI 63

Query: 62  LISQRKSDDHNVDPISTSKKEIFFSLCNSPVLIDDGNKSLSLDKWSGRKCIMLGARDLSV 121
           LIS+RK  + N DPIS+SKK IFFSLC++P++IDDGNKS SLDK SG+KCIMLGAR+LS+
Sbjct: 64  LISRRKPSNLNFDPISSSKKGIFFSLCDTPLIIDDGNKSFSLDKCSGKKCIMLGARELSI 123

Query: 122 IWGDKSLYWTWFHHPLSRFKVYEIEANHILYLFVFFLSLPILILCFFNVGVFAEVAVLLN 181
           IW D   YWTW  HP SRF      A   + L V++L                       
Sbjct: 124 IWSDMPAYWTWEPHPESRF------AEVAVLLNVWWLE---------------------- 183

Query: 182 VWWLEIRGRINCKMLSPKTTYAVYFVFRMRNHNYVGFDVNLADATVGIIGTENHHRSVCL 241
                I+G+++CKMLSP TTYA YFVF+M    Y GFD+  ADA V I+  E     VCL
Sbjct: 184 -----IKGKLSCKMLSPATTYAAYFVFKMDERRYYGFDIVAADAAVAIVDGECCSSRVCL 243

Query: 242 DPHLDN--PRRWQPRRNGPWLMRPLPGPSLDMLGLEWPQERRDGWFEIELGEFESGDGDD 301
           DP L+N  P+R   RR  P L R   G S  M   + PQER DGWFEIELGE  +  GDD
Sbjct: 244 DPFLENAPPKR---RRRTPCLRRNPLGNS--MSRAKQPQERHDGWFEIELGELRNNGGDD 298

Query: 302 EVEMAVMEVKGHSAKSGLVVEGIEIRPKHSWSL 333
            VE  + EV  + +KSGL+V+GI+IRPK S  L
Sbjct: 304 VVEFFLKEVNCNYSKSGLIVQGIDIRPKVSRDL 298

BLAST of Lsi06G011220 vs. TrEMBL
Match: A0A0A0LZQ3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G421910 PE=4 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 2.1e-73
Identity = 158/333 (47.45%), Postives = 211/333 (63.36%), Query Frame = 1

Query: 2   ETEDEEIGDLSFMPEGVIANLLSLTSPIDACRSAAVSPIFNAAAQSDFVWDRFLPTDWEV 61
           E+E +E+ + S +PEGVIAN+LSLTSP+D CR +AVS  F+AAAQSD  W+RF+P+D+ +
Sbjct: 20  ESEGKEMVEFSSLPEGVIANILSLTSPLDNCRLSAVSWTFHAAAQSDVAWERFIPSDYHL 79

Query: 62  LISQRKSDDHNVDPISTSKKEIFFSLCNSPVLIDDGNKSLSLDKWSGRKCIMLGARDLSV 121
           LIS+       ++P S+SKK+IFFSL ++P+LIDDG +S SLD WSG+KCIMLGARDLS+
Sbjct: 80  LISR-------MNPTSSSKKDIFFSLSDTPLLIDDGKQSFSLDIWSGKKCIMLGARDLSI 139

Query: 122 IWGDKSLYWTWFHHPLSRFKVYEIEANHILYLFVFFLSLPILILCFFNVGV---FAEVAV 181
              D S  W W  H  SR                              VG+   F EV V
Sbjct: 140 SGSDTSSLWKWVPHQGSR------------------------------VGLRNRFGEVVV 199

Query: 182 LLNVWWLEIRGRINCKMLSPKTTYAVYFVFRMRNHNYVGFDVNLADATVGIIGTENHHRS 241
           LL++  LE+ G+I+C+MLSP TTYA YFVF+M+   Y GF+++  +A +G++G + H ++
Sbjct: 200 LLDICLLEVSGKISCRMLSPATTYAAYFVFKMKKRKYYGFNLDPVEAMLGVVGDDCHGKN 259

Query: 242 VCLDPHLDNPRRWQPRRNGPWLMRPLP--GPSLDMLGLEWPQERRDGWFEIELGEFESGD 301
           VCLDP LD+P          WL + LP    + +M   E P  R DG FEIELGEF++  
Sbjct: 260 VCLDPDLDSP---------SWLHQFLPWAESNQNMSEFEQPNWRSDGLFEIELGEFQTNG 306

Query: 302 GDDEVEMAVMEVKGHSAKSGLVVEGIEIRPKHS 330
            DDEVE+ + E    S KSGLVV GI+IRPK S
Sbjct: 320 KDDEVEVILREDNRCSPKSGLVVVGIDIRPKTS 306

BLAST of Lsi06G011220 vs. TAIR10
Match: AT2G02360.1 (AT2G02360.1 phloem protein 2-B10)

HSP 1 Score: 199.1 bits (505), Expect = 4.4e-51
Identity = 116/315 (36.83%), Postives = 167/315 (53.02%), Query Frame = 1

Query: 15  PEGVIANLLSLTSPIDACRSAAVSPIFNAAAQSDFVWDRFLPTDWEVLISQRKSDDHNVD 74
           PE  I+ ++S T+P DAC +A VS  F +  +SD +W++FLP D+E LI   +       
Sbjct: 18  PEDCISYIISFTNPRDACVAATVSKTFESTVKSDIIWEKFLPADYESLIPPSR------- 77

Query: 75  PISTSKKEIFFSLCNSPVLIDDGNKSLSLDKWSGRKCIMLGARDLSVIWGDKSLYWTWFH 134
            + +SKKE++FSLCN PVL DD  KS+ L+K SG++C+ML A +LS+IWGD   YW    
Sbjct: 78  -VFSSKKELYFSLCNDPVLFDDDKKSVWLEKASGKRCLMLSAMNLSIIWGDNPQYW---- 137

Query: 135 HPLSRFKVYEIEANHILYLFVFFLSLPILILCFFNVGVFAEVAVLLNVWWLEIRGRINCK 194
                                 ++ +P           F +VA L +V W EIRGR N +
Sbjct: 138 ---------------------QWIPIP--------ESRFEKVAKLRDVCWFEIRGRTNTR 197

Query: 195 MLSPKTTYAVYFVFRMRNHNYVGFDVNLADATVGIIGTENHHRSVCLDPHLDNPRRWQPR 254
           +LSP+T Y+ Y VF+  +  Y GF     +A VG++G E   R +C    +   RR    
Sbjct: 198 VLSPRTRYSAYIVFKGVDKCY-GFQNVAIEAAVGVVGQEPSRRLICFSEAIRRGRR---- 257

Query: 255 RNGPWLMRPLPGPSLDMLGLEWPQERRDGWFEIELGEFESGDG---DDEVEMAVMEVKGH 314
                              +  P++R DGW EIELGEF +  G   +DE+EM+ +E K  
Sbjct: 258 ------------------NVVKPKQREDGWMEIELGEFFNDGGIMDNDEIEMSALETKQL 268

Query: 315 SAKSGLVVEGIEIRP 327
           + K GL+++GIEIRP
Sbjct: 318 NRKCGLIIQGIEIRP 268

BLAST of Lsi06G011220 vs. TAIR10
Match: AT2G02240.1 (AT2G02240.1 F-box family protein)

HSP 1 Score: 193.0 bits (489), Expect = 3.2e-49
Identity = 114/317 (35.96%), Postives = 173/317 (54.57%), Query Frame = 1

Query: 14  MPEGVIANLLSLTSPIDACRSAAVSPIFNAAAQSDFVWDRFLPTDWEVLISQRKSDDHNV 73
           +PE  I+N++S TSP DAC +A+VS  F +A  SD VWD+FLP ++E L+S+ +      
Sbjct: 64  LPEDCISNIISFTSPRDACVAASVSKTFESAVSSDCVWDKFLPPEYESLVSRSR------ 123

Query: 74  DPISTSKKEIFFSLCNSPVLIDDGNKSLSLDKWSGRKCIMLGARDLSVIWGDKSLYWTWF 133
             +  SKKE++F+LC++PVLI+DG KS  L+K SG++CIML +++L + WG    YW W 
Sbjct: 124 --VFASKKELYFALCHNPVLIEDGKKSFWLEKASGKRCIMLSSKELWITWGSSPEYWQWI 183

Query: 134 HHPLSRF-KVYEIEANHILYLFVFFLSLPILILCFFNVGVFAEVAVLLNVWWLEIRGRIN 193
             P SRF K+ E                 +L +C+F                 EIRG+ +
Sbjct: 184 SIPESRFNKIAE-----------------LLDVCWF-----------------EIRGKTS 243

Query: 194 CKMLSPKTTYAVYFVFRMRNHNYVGFDVNLADATVGIIGTENHHRSVCLDPHLDNPRRWQ 253
            ++LSP T Y+ Y VF+ ++    G      +  +G++G E+  R +    +   PR  +
Sbjct: 244 ARVLSPGTRYSAYIVFKTKD-RCPGLGHLPVEVGLGLVGQESSKRFI----YFIGPRDRR 303

Query: 254 PRRNGPWLMRPLPGPSLDMLGLEWPQERRDGWFEIELGEFESGDGDDEVEMAVMEVKGHS 313
            RR              +   +  P +R DGW E ELGEF + +  DE+E +V+E+K  S
Sbjct: 304 GRR--------------ETRDVTKPDQREDGWMEAELGEFFNEERCDEIEFSVIEIKSPS 319

Query: 314 AKSGLVVEGIEIRPKHS 330
            KSGL+++GIE RP  S
Sbjct: 364 WKSGLIIQGIEFRPTKS 319

BLAST of Lsi06G011220 vs. TAIR10
Match: AT2G02340.1 (AT2G02340.1 phloem protein 2-B8)

HSP 1 Score: 185.3 bits (469), Expect = 6.6e-47
Identity = 114/325 (35.08%), Postives = 176/325 (54.15%), Query Frame = 1

Query: 8   IGDLSFMPEGVIANLLSLTSPIDACRSAAVSPIFNAAAQSDFVWDRFLPTDWEVLISQRK 67
           + +L  +PE  ++ ++S TSP DAC  A+VS  F +A +SD VW++F+P ++E LISQ +
Sbjct: 33  VAELDDLPEECVSIIVSFTSPQDACVLASVSKTFASAVKSDIVWEKFIPPEYESLISQSR 92

Query: 68  SDDHNVDPISTSKKEIFFSLCNSPVLIDDGNKSLSLDKWSGRKCIMLGARDLSVIWGDKS 127
           +          SKKE++F+LC+  VLIDDG KSL ++K + ++CIM+ A +L++      
Sbjct: 93  AFKF------LSKKELYFALCDKSVLIDDGKKSLWIEKANAKRCIMISAMNLAI------ 152

Query: 128 LYWTWFHHPLSRFKVYEIEANHILYLFVFFLSLPILILCFFNVGVFAEVAVLLNVWWLEI 187
               W + P S   + + +A                         F  VA LL V   EI
Sbjct: 153 ---AWGNSPQSWRWIPDPQAR------------------------FETVAELLEVCLFEI 212

Query: 188 RGRINCKMLSPKTTYAVYFVFRMRNHNYVGFDVNLADATVGIIG---TENHHRSVCLDPH 247
           RGRIN +++SPKT Y+ Y V++  N  Y GF+    +  VG++G    E+  R +C D  
Sbjct: 213 RGRINSRVISPKTRYSAYIVYKKLNICY-GFENVAVEVVVGVVGQDLEESCRRYICFDET 272

Query: 248 LDNPRRWQPRRNGPWLMRPLPGPSLDMLGLEWPQERRDGWFEIELGEFESGDG---DDEV 307
           +D   +++ R  G  L++              P+ R+DGW EI++GEF +  G   DDE+
Sbjct: 273 MD--EQFRRRDRGKNLVK--------------PERRKDGWMEIKIGEFFNEGGLLNDDEI 301

Query: 308 EMAVMEVKGHSAKSGLVVEGIEIRP 327
           EM  +E K    K GL+++GIEIRP
Sbjct: 333 EMVALEAKQRHWKRGLIIQGIEIRP 301

BLAST of Lsi06G011220 vs. TAIR10
Match: AT2G02230.1 (AT2G02230.1 phloem protein 2-B1)

HSP 1 Score: 183.3 bits (464), Expect = 2.5e-46
Identity = 119/342 (34.80%), Postives = 177/342 (51.75%), Query Frame = 1

Query: 14  MPEGVIANLLSLTSPIDACRSAAVSPIFNAAAQSDFVWDRFLPTDWEVLISQRKSDDHNV 73
           +PE  I+ ++S TSP DAC  A+VS    +AAQSD VW+ FLP+++  L+ Q  S +H  
Sbjct: 35  LPEDCISKVISHTSPRDACVVASVSKSVKSAAQSDLVWEMFLPSEYSSLVLQ--SANH-- 94

Query: 74  DPISTSKKEIFFSLCNSPVLIDDGNKSLSLDKWSGRKCIMLGARDLSVIWGDKSLYWTWF 133
                SKKEIF SL ++ VL+++G KS  ++K SG+KC ML A +L++IWGD        
Sbjct: 95  ----LSKKEIFLSLADNSVLVENGKKSFWVEKASGKKCYMLSAMELTIIWGDSP------ 154

Query: 134 HHPLSRFKVYEIEANHILYLFVFFLSLPILILCFFNVGVFAEVAVLLNVWWLEIRGRINC 193
                               +  ++++P           F +VA L NV W E+RG+I+C
Sbjct: 155 -------------------AYWKWITVP--------ESKFEKVAELRNVCWFEVRGKISC 214

Query: 194 KMLSPKTTYAVYFVFRMRNHNYVGFDVNLADATVGIIGTENHHRSVCLDPHLDNPRRWQP 253
            MLS  T Y+VY VF+  N    GFD+   +A VG +G     +SV  +    + R    
Sbjct: 215 GMLSKGTHYSVYVVFKTANGRSYGFDLVPVEAGVGFVGKVATKKSVYFESGNADSRSATS 274

Query: 254 RRNG--------------PWLM------RPLPGPSLDMLGLEWPQERRDGWFEIELGEFE 313
             +G              PW+         + G     + +  P+ER DGW E+ELG+F 
Sbjct: 275 HYSGISYAMVSRAFRMRRPWMQVQREEEEEVEGERERGMNVVGPKERVDGWSEVELGKFY 334

Query: 314 ------SGDGDDEVEMAVMEVKGHSAKSGLVVEGIEIRPKHS 330
                   DG DE+E+++ME +  + KSGL+++GIEIRP+ S
Sbjct: 335 INNGGCGDDGSDEIEISIMETQNGNWKSGLIIQGIEIRPERS 335

BLAST of Lsi06G011220 vs. TAIR10
Match: AT2G02250.1 (AT2G02250.1 phloem protein 2-B2)

HSP 1 Score: 177.9 bits (450), Expect = 1.1e-44
Identity = 107/317 (33.75%), Postives = 164/317 (51.74%), Query Frame = 1

Query: 14  MPEGVIANLLSLTSPIDACRSAAVSPIFNAAAQSDFVWDRFLPTDWEVLISQRKSDDHNV 73
           +PE  I+N++S TSP DAC +A+VS  F +A  SD VWD+FLP+D+  L+   +      
Sbjct: 50  LPEDCISNIISFTSPRDACVAASVSKTFESAVNSDSVWDKFLPSDYSSLVPPSR------ 109

Query: 74  DPISTSKKEIFFSLCNSPVLIDDGNKSLSLDKWSGRKCIMLG-ARDLSVIWGDKSLYWTW 133
             + +SKKE++F++C++PVL++DG KS  L+K +G+KC ML   + + + W     YW W
Sbjct: 110 --VFSSKKELYFAICDNPVLVEDGGKSFWLEKENGKKCFMLSPKKSMWITWVSTPQYWRW 169

Query: 134 FHHPLSRFKVYEIEANHILYLFVFFLSLPILILCFFNVGVFAEVAVLLNVWWLEIRGRIN 193
              P +RF+                  +P L+                 V W E+RG +N
Sbjct: 170 ISIPEARFE-----------------EVPELLN----------------VCWFEVRGGMN 229

Query: 194 CKMLSPKTTYAVYFVFRMRNHNYVGFDVNLADATVGIIGTENHHRSVCLDPHLDNPRRWQ 253
            K LSP T Y+ Y VF+ +N      DV + +ATVG++G E+  R +      D  R  +
Sbjct: 230 TKELSPGTRYSAYIVFKTKNGCPNLGDVPV-EATVGLVGQESSQRHIYFVGPSDQRRDRE 289

Query: 254 PRRNGPWLMRPLPGPSLDMLGLEWPQERRDGWFEIELGEFESGDGDDEVEMAVMEVKGHS 313
            R                   +  P +R+DGW E ELG+F +  G D V+ +++E+K   
Sbjct: 290 TR------------------DVTRPTKRKDGWMEAELGQFFNESGCDVVDTSILEIKTPY 306

Query: 314 AKSGLVVEGIEIRPKHS 330
            K GL+++GIE RP  S
Sbjct: 350 WKRGLIIQGIEFRPTKS 306

BLAST of Lsi06G011220 vs. NCBI nr
Match: gi|659107347|ref|XP_008453628.1| (PREDICTED: F-box protein PP2-B10-like [Cucumis melo])

HSP 1 Score: 447.2 bits (1149), Expect = 2.7e-122
Identity = 224/339 (66.08%), Postives = 259/339 (76.40%), Query Frame = 1

Query: 4   EDEEIGDLSFMPEGVIANLLSLTSPIDACRSAAVSPIFNAAAQSDFVWDRFLPTDWEVLI 63
           EDEEIGDLSF+PEGVIAN+LS T+PIDAC +AAVS +FNAAAQSDFVWDRFLP DW+VLI
Sbjct: 6   EDEEIGDLSFLPEGVIANVLSFTTPIDACTAAAVSRVFNAAAQSDFVWDRFLPPDWDVLI 65

Query: 64  SQRKSDDHNVDPISTSKKEIFFSLCNSPVLIDDGNKSLSLDKWSGRKCIMLGARDLSVIW 123
           S RKS     DP+S+SKK+IFFSLCN+PV IDD NKSLSLD+ SG+KCIM+GAR LSV+W
Sbjct: 66  SHRKS----FDPLSSSKKDIFFSLCNNPVPIDDRNKSLSLDRQSGKKCIMVGARGLSVVW 125

Query: 124 GDKSLYWTWFHHPLSRFKVYEIEANHILYLFVFFLSLPILILCFFNVGVFAEVAVLLNVW 183
           GD S YW+W  HP SRF      A   + L V++L L                       
Sbjct: 126 GDTSRYWSWDRHPRSRF------AEVAVLLKVWWLEL----------------------- 185

Query: 184 WLEIRGRINCKMLSPKTTYAVYFVFRMRNHNYVGFDVNLADATVGIIGTENH-HRSVCLD 243
               RGRI+C +LSPKTTYAVY VFRM+N NY GF+   ADATVGI+GTENH  RSVCLD
Sbjct: 186 ----RGRISCNILSPKTTYAVYLVFRMKNCNYEGFNFYPADATVGIVGTENHGRRSVCLD 245

Query: 244 PHLDNPRRWQPRRNGPWLMRPLPGPSLDMLGLEWPQERRDGWFEIELGEFESGDGDDEVE 303
           PHLDNP +W+     P LM PLPGPS++MLGLEWPQERRDGWF+IELGEFESGDG DEVE
Sbjct: 246 PHLDNPLKWRRGYLEPRLMTPLPGPSVEMLGLEWPQERRDGWFQIELGEFESGDGGDEVE 305

Query: 304 MAVMEVKGHSAKSGLVVEGIEIRPKHSWSLLKPWYCKIA 342
           +A+MEVKGH  K+GL+VEG EIRPKH W+LLKPWYC++A
Sbjct: 306 IALMEVKGHFVKTGLIVEGFEIRPKHCWTLLKPWYCRVA 307

BLAST of Lsi06G011220 vs. NCBI nr
Match: gi|778660698|ref|XP_011656739.1| (PREDICTED: F-box protein PP2-B10-like [Cucumis sativus])

HSP 1 Score: 443.4 bits (1139), Expect = 3.8e-121
Identity = 223/340 (65.59%), Postives = 262/340 (77.06%), Query Frame = 1

Query: 4   EDEEIGDLSFMPEGVIANLLSLTSPIDACRSAAVSPIFNAAAQSDFVWDRFLPTDWEVLI 63
           ED+EIGDLSF+PEGVIAN+LS T+PIDAC +AAVS +FNAAAQSDFVWDRFLP DW+VLI
Sbjct: 17  EDQEIGDLSFLPEGVIANVLSFTTPIDACTAAAVSRVFNAAAQSDFVWDRFLPPDWDVLI 76

Query: 64  SQRKSDDHNVDPISTSKKEIFFSLCNSPVLIDDGNKSLSLDKWSGRKCIMLGARDLSVIW 123
           S RKS     DP+S+SKK+IFFSLCN+PVLIDD NKSLSLD+ SG+KCIMLGAR LS++W
Sbjct: 77  SYRKS----FDPLSSSKKDIFFSLCNNPVLIDDRNKSLSLDRQSGKKCIMLGARGLSLLW 136

Query: 124 GDKSLYWTWFHHPLSRFKVYEIEANHILYLFVFFLSLPILILCFFNVGVFAEVAVLLNVW 183
           GD S YW+W  HP SRF      A   + L  ++L L                       
Sbjct: 137 GDTSRYWSWDRHPRSRF------AEVAVLLKAWWLEL----------------------- 196

Query: 184 WLEIRGRINCKMLSPKTTYAVYFVFRMRNHNYVGFDVNLADATVGIIGTENH-HRSVCLD 243
               RGRI+CK+LSP+TTYAVYFVF+M+N NY GF+   ADATVGI+GTENH  RSVCLD
Sbjct: 197 ----RGRISCKILSPRTTYAVYFVFKMKNCNYEGFNFYPADATVGIVGTENHGRRSVCLD 256

Query: 244 PHLDNPRRWQPRRNGPWLMRPLPGPSLDMLGLEWPQERRDGWFEIELGEFESGDGDDEVE 303
           PHLDNPR+W   R G     P PGPS++MLGLEWPQERRDGWF+IELGEFESGDG DEVE
Sbjct: 257 PHLDNPRQW---RRG---FLPSPGPSVEMLGLEWPQERRDGWFQIELGEFESGDGADEVE 313

Query: 304 MAVMEVKGHSAKSGLVVEGIEIRPKHSWSLLKPWYCKIAS 343
           +A+MEVKGHS K+GL+VEG EIRPKHSW+LLKPW+C++A+
Sbjct: 317 IALMEVKGHSTKTGLIVEGFEIRPKHSWTLLKPWHCRVAT 313

BLAST of Lsi06G011220 vs. NCBI nr
Match: gi|659107345|ref|XP_008453627.1| (PREDICTED: F-box protein PP2-B10-like [Cucumis melo])

HSP 1 Score: 354.0 bits (907), Expect = 3.1e-94
Identity = 187/332 (56.33%), Postives = 226/332 (68.07%), Query Frame = 1

Query: 4   EDEEIG-----DLSFMPEGVIANLLSLTSPIDACRSAAVSPIFNAAAQSDFVWDRFLPTD 63
           E EE G     DLS +PEGV+A +LSLT+P DAC  +AVS  F+AAAQSD VW+ FLP D
Sbjct: 2   EGEEKGKATFSDLSSLPEGVVAKILSLTTPPDACVLSAVSKTFHAAAQSDVVWNEFLPAD 61

Query: 64  WEVLISQRKSDDHNVDPISTSKKEIFFSLCNSPVLIDDGNKSLSLDKWSGRKCIMLGARD 123
           WE+LIS+ K +    DP+S+ KK+IFFSLC  PVLIDDGNKS SL+KW+G+KCIMLGARD
Sbjct: 62  WELLISRLKPNKLKFDPVSSPKKDIFFSLCYFPVLIDDGNKSFSLEKWTGKKCIMLGARD 121

Query: 124 LSVIWGDKSLYWTWFHHPLSRFKVYEIEANHILYLFVFFLSLPILILCFFNVGVFAEVAV 183
           LS+ WG+ S YWTW  HP SRF     E   ++Y+                         
Sbjct: 122 LSITWGENSDYWTWEDHPDSRF----AEVAVLIYV------------------------- 181

Query: 184 LLNVWWLEIRGRINCKMLSPKTTYAVYFVFRMRNHNYVGFDVNLADATVGIIGTENHHRS 243
               WWLEIRGR++C+MLSPKT YAVYFVF +   +Y GF+++  DATVGI+G ENH +S
Sbjct: 182 ----WWLEIRGRLSCRMLSPKTVYAVYFVFNIEESSYYGFNIDPPDATVGILGHENHPKS 241

Query: 244 VCLDPHLDNP--RRWQPRRNGPWLMRPLPGPSLDMLGLEWPQERRDGWFEIELGEFESGD 303
           VCLDP+LD P  R+ Q +R  PW  RP    S  M  L+ P +R DGWFEIELGEF SGD
Sbjct: 242 VCLDPYLDQPQQRQRQRQRRAPWERRP---QSEHMPALDRPHKRHDGWFEIELGEFISGD 297

Query: 304 G-DDEVEMAVMEVKGHSAKSGLVVEGIEIRPK 328
             DDE+EMA+ EVKG S+K GLVVEGIEIRPK
Sbjct: 302 DVDDELEMALKEVKGSSSKGGLVVEGIEIRPK 297

BLAST of Lsi06G011220 vs. NCBI nr
Match: gi|778660693|ref|XP_004146356.2| (PREDICTED: F-box protein PP2-B10 [Cucumis sativus])

HSP 1 Score: 326.2 bits (835), Expect = 6.8e-86
Identity = 173/325 (53.23%), Postives = 215/325 (66.15%), Query Frame = 1

Query: 4   EDEEIGDLSFMPEGVIANLLSLTSPIDACRSAAVSPIFNAAAQSDFVWDRFLPTDWEVLI 63
           E E++ D S +PEGV+A +LSLT+P DAC S+AVS IF+AAAQSD VW++FLPTDWE+ I
Sbjct: 11  EGEQLSDFSSLPEGVVAKILSLTTPPDACVSSAVSKIFHAAAQSDIVWNQFLPTDWELFI 70

Query: 64  SQRKSDDHNVDPISTSKKEIFFSLCNSPVLIDDGNKSLSLDKWSGRKCIMLGARDLSVIW 123
           S+   +    DPI + KK+IFFSLC  PVLIDDGNKS SL+KWSG+KCIM+GARDLS+ W
Sbjct: 71  SRSNPNKLKFDPIFSPKKDIFFSLCYFPVLIDDGNKSFSLEKWSGKKCIMVGARDLSITW 130

Query: 124 GDKSLYWTWFHHPLSRFKVYEIEANHILYLFVFFLSLPILILCFFNVGVFAEVAVLLNVW 183
           G+ S   +W  HP SRF                     + +L F               W
Sbjct: 131 GENS-DSSWEDHPDSRF-------------------AEVAVLLFE--------------W 190

Query: 184 WLEIRGRINCKMLSPKTTYAVYFVFRMRNHNYVGFDVNLADATVGIIGTENHHRSVCLDP 243
           W EIRGR++C+MLSPKT YA YF+F++   +Y GF+++  +ATVGIIG ENH +SVCLDP
Sbjct: 191 WFEIRGRLSCRMLSPKTVYAAYFLFKLGERSYSGFNIDPTNATVGIIGHENHPKSVCLDP 250

Query: 244 HLDNPRRWQPRRNGPWLMRPLPGPSLDMLGLEWPQERRDGWFEIELGEFESGD-GDDEVE 303
           ++D P         P   +        M GLE P +R DGWFEIELGEF  GD GDDE+E
Sbjct: 251 YVDQPLYLDQLIQWPQWPQWRRQFERRMAGLERPHKRHDGWFEIELGEFIGGDHGDDELE 301

Query: 304 MAVMEVKGHSAKSGLVVEGIEIRPK 328
           MA+ EVKG S+K GLVVEGIEIRPK
Sbjct: 311 MALKEVKGSSSKGGLVVEGIEIRPK 301

BLAST of Lsi06G011220 vs. NCBI nr
Match: gi|659107353|ref|XP_008453631.1| (PREDICTED: F-box protein PP2-B10-like [Cucumis melo])

HSP 1 Score: 323.2 bits (827), Expect = 5.8e-85
Identity = 176/331 (53.17%), Postives = 212/331 (64.05%), Query Frame = 1

Query: 2   ETEDEEIGDLSFMPEGVIANLLSLTSPIDACRSAAVSPIFNAAAQSDFVWDRFLPTDWEV 61
           E + EEI D S +PEGV+A +LS T+P DACRS+ VS  F AAAQSD VWD FLPTDWE+
Sbjct: 4   EEKGEEISDFSSLPEGVVAKILSFTTPSDACRSSVVSTTFVAAAQSDIVWDSFLPTDWEI 63

Query: 62  LISQRKSDDHNVDPISTSKKEIFFSLCNSPVLIDDGNKSLSLDKWSGRKCIMLGARDLSV 121
           LIS+RK  + N DPIS+SKKEIFFSLC++P++IDDGNKS SLDKWSG+KCIMLGAR+LS+
Sbjct: 64  LISRRKPSNLNFDPISSSKKEIFFSLCDTPLIIDDGNKSFSLDKWSGKKCIMLGARELSI 123

Query: 122 IWGDKSLYWTWFHHPLSRFKVYEIEANHILYLFVFFLSLPILILCFFNVGVFAEVAVLLN 181
           IW D   YWTW  HP SRF      A   + L V++L                       
Sbjct: 124 IWSDMPTYWTWEPHPESRF------AEVAVLLNVWWLE---------------------- 183

Query: 182 VWWLEIRGRINCKMLSPKTTYAVYFVFRMRNHNYVGFDVNLADATVGIIGTENHHRSVCL 241
                I+G+++CKMLSP TTYA YFVF+M    Y GFD+  ADA V I+  E     VCL
Sbjct: 184 -----IKGKLSCKMLSPATTYAAYFVFKMDERRYYGFDIVAADAAVAIVDGECCSSRVCL 243

Query: 242 DPHLDNPRRWQPRRNGPWLMRPLPGPSLDMLGLEWPQERRDGWFEIELGEFESGDGDDEV 301
           DP LD       RR  P L R   G S  M   + PQER DGWFE+ELGE  +  GDDEV
Sbjct: 244 DPFLDIAP--PKRRRTPCLRRNPLGNS--MSRAKQPQERHDGWFELELGELHNNGGDDEV 297

Query: 302 EMAVMEVKGHSAKSGLVVEGIEIRPKHSWSL 333
           E  + EV  + +KSGL+V+GI+IRPK S  L
Sbjct: 304 EFFLKEVNCNYSKSGLIVQGIDIRPKISRDL 297

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P2B10_ARATH7.9e-5036.83F-box protein PP2-B10 OS=Arabidopsis thaliana GN=PP2B10 PE=1 SV=1[more]
FB95_ARATH5.7e-4835.96F-box protein At2g02240 OS=Arabidopsis thaliana GN=At2g02240 PE=2 SV=1[more]
PP2B8_ARATH1.2e-4535.08Putative F-box protein PP2-B8 OS=Arabidopsis thaliana GN=PP2B8 PE=3 SV=1[more]
PP2B1_ARATH4.5e-4534.80F-box protein PP2-B1 OS=Arabidopsis thaliana GN=PP2B1 PE=1 SV=1[more]
SKIP3_ARATH1.0e-4437.19F-box protein SKIP3 OS=Arabidopsis thaliana GN=SKIP3 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0LUI2_CUCSA2.7e-12165.59Uncharacterized protein OS=Cucumis sativus GN=Csa_1G421930 PE=4 SV=1[more]
A0A0A0LX65_CUCSA4.8e-8653.23Uncharacterized protein OS=Cucumis sativus GN=Csa_1G421920 PE=4 SV=1[more]
A0A0A0LU29_CUCSA2.6e-8452.22Uncharacterized protein OS=Cucumis sativus GN=Csa_1G421900 PE=4 SV=1[more]
A0A0A0LWS1_CUCSA1.4e-8252.55Uncharacterized protein OS=Cucumis sativus GN=Csa_1G421890 PE=4 SV=1[more]
A0A0A0LZQ3_CUCSA2.1e-7347.45Uncharacterized protein OS=Cucumis sativus GN=Csa_1G421910 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G02360.14.4e-5136.83 phloem protein 2-B10[more]
AT2G02240.13.2e-4935.96 F-box family protein[more]
AT2G02340.16.6e-4735.08 phloem protein 2-B8[more]
AT2G02230.12.5e-4634.80 phloem protein 2-B1[more]
AT2G02250.11.1e-4433.75 phloem protein 2-B2[more]
Match NameE-valueIdentityDescription
gi|659107347|ref|XP_008453628.1|2.7e-12266.08PREDICTED: F-box protein PP2-B10-like [Cucumis melo][more]
gi|778660698|ref|XP_011656739.1|3.8e-12165.59PREDICTED: F-box protein PP2-B10-like [Cucumis sativus][more]
gi|659107345|ref|XP_008453627.1|3.1e-9456.33PREDICTED: F-box protein PP2-B10-like [Cucumis melo][more]
gi|778660693|ref|XP_004146356.2|6.8e-8653.23PREDICTED: F-box protein PP2-B10 [Cucumis sativus][more]
gi|659107353|ref|XP_008453631.1|5.8e-8553.17PREDICTED: F-box protein PP2-B10-like [Cucumis melo][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005515protein binding
Vocabulary: INTERPRO
TermDefinition
IPR025886PP2-like
IPR001810F-box_dom
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005515 protein binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi06G011220.1Lsi06G011220.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001810F-box domainunknownSSF81383F-box domaincoord: 9..114
score: 1.2
IPR025886Phloem protein 2-likePFAMPF14299PP2coord: 110..141
score: 4.7E-7coord: 173..326
score: 3.0
NoneNo IPR availableGENE3DG3DSA:1.20.1280.50coord: 11..72
score: 4.
NoneNo IPR availablePANTHERPTHR32278FAMILY NOT NAMEDcoord: 2..138
score: 2.9E-117coord: 172..335
score: 2.9E
NoneNo IPR availablePANTHERPTHR32278:SF6F-BOX PROTEIN PP2-B1-RELATEDcoord: 2..138
score: 2.9E-117coord: 172..335
score: 2.9E