Lsi06G010220 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi06G010220
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	chr06 : 20391770 .. 20396199 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAATTCTCTTACTCTTAATTATTAAAAAAAATGATTGGTTTCAGTTTTGGTTTATTTATGGCACTTTCTTTAAGTATCTCTATCTACATCCATGGAATCCCATCGACACAAACATGGCAGAAAATTCTGACCTCACACAAACACTCCAAGAAAAGCTCCTCATCAACGGCGGCGATACGCCGGAAAGTTATATTTACAAAGGCGGCTACTGCGGTGGAGATTCCAACAATAATATCAATCCGCTTCCATTAGCAGAGATTCCGGTCGTTGACTGTTCTCGACTTTTATCGACGCCGGCGGGCGAGGCTCCGTTGAACGAGCTCCGGTTGGCTCTCAGTACTTGGGGATGTTTTCAGGTCAGTTTTCATTGGATCTCAACTCAACGACCCATCTCATGATTTTAATCAATTAAATTTTTGTTATTAGTTATCAACCCACAATTTTGTTCTAATTTTCTGCTCTAATTTCTAATTATAGTCTTTAAAAATTTTTTATTAAAAAAACACACACAAACTTGAATTTCTTGCAAAATTTTGAAGAAAGAAATGAAAACTATCATTTTTGTTTTATTTTTCAAAACTTGTTGGTTGTTTACATCATTCGTGGAGATGGGATGATAAAGCATACAAATTTAGAATTAGTCTTAAGTTTATTTATAAAAAAACAAAAACCATTTTCTAAAAAAACCACAAAAAAAAAAAAAAAAAAAAAACCAAAACTAAGGTCTGATGGGCAACTATTTGGACCCGTTTGATTATGTTGATGCCAAAATTCGAACGAAAAAAATACACCTTGACTTCATGACAACAATGGTAAATTTGTAATTTGAAGAAGGAAGGACCTAGAAAATAAAGAAAAATAATTCTGATATCAAAGTCAGAAAATTATAGAATGGGGAAAGGGCAAAAGTTTCCGTTCTAGAAGTTCTAGATCTCAGATTTAGTTCCCGTTACCTCATATGGAGTTATGTACTTACAGGCAGAATGAGATGAAGTAAACCCTTTTTTCTTTTAGGATTGGATGAGATGGATTTTTCCTATCATAAGTAAGTTAAGATTAGATCTGATTTGATAAATGATTAGTTGATACTTTGACTTATTTGAATTCAAGTTCACTTTATCTTGTATTTATCTTTCCATATCTTTTTCTAATTCTTGACTTATTTGTCAGGTCCAATTTCACCTAGAACAGATAACCATTTGAATTTTTTATTTTTTTTGTTTTTGAAAATTAAGCTTAAACACTCCTTTCACCTCTAAATGTTTTTATTTGTTATCAAATTTTTATCAATATCTAAAAAACCAAGGCAAAATTTGAAAACTAAAAAAAAAAAAAGAAAAAAAAAATTCAAAACTTACTTTTGTTTTTAGAATTTGGCTAACAATTCAACTCTTGTATTTAAGAAAGATGCAAATCATTGTAAGACATTGAGACCCTAATTTTTAGAAGAAAGAAAGAAAAAATAGTTTTCCTAACCTTGTTTTTTTCTATAAAAAAATTGACTAAGAATTCAATTTTTCTTTTCAAGAAATATAGTTTAGGAGAAAAACAAGAATAAATTTCCAAAAAAAAAAAAAAAATAGAAAATTCATTATTCTTTAAGCGCCCGTTTGAATTGACTAGAGAAGAAAGTATTTTCAAAAAACTCATTTTTATTTAAATTTTTTTAACAAAAAGGGTTTAAAATAGACTTCCAAAGCTATTTTAAGTGGTTTTCAAACACTCTAATTTTTTCTAAAATGACTTATTTTTTAAATTAAATACTTAAAAATGTATTCCAAACACACCTTAAATAGTTATGAAATGAAAAGTAAACGAAGTGATTATCGTAAATTAATTTGGAGAAATTAATGAAGTTAGTGTGTGGTTTCAGGCGATTAATCACAATATTTCAAGTTCATTTTTGGAGAAGATGAGAAAATTAAGCGAGCAATTTTTCTCACTGCCTATTGAAGAGAAGAAGAGATGTAGAAGAGAAGTTGATGGAATGGAAGGATATGGAAATGATTTGATTTTATCAAACCAACAAATTTTAGATTGGACTGATCGGTTGTATCTTATAGTAAATCCTCAGGATCAAAGAAAACTCAACTTTTGGCCTCTAAATCCTCAGTCTTTCAGGTCCATTTCTCGTTCATTTAAACTCTAACTTTTTATAAGTACGTCAATTTATATTATTTGTTGATGATTTAAATCAGCTCAAACTTATCTAAATAAAACAATTCTTCTCAAAAAATATCCGCAATAAAAATTATCATACAATTCTTTATAAATGTGCAAAAGAAGTAGAGGAAGACAAGTAGATGAAAATCGTTCAAATTTTAATAAATATTTGCTACTATTTGTTTTTGGGGAATTTTTAAAAATAAAAAATAAAGGAAACTATTTACACAAAATAGAAAAAAAATTTAGATAGTTGTGATAAACGTTGATAGAAGTCTATCAGGGTCTATGAGTGATAGAGATAATAGAAGTCTATCAATGTCTATTCGTGTTTTTCTTTTGCTATTTTCTATAAATAGTTTGATATTTTTTTAATATGTGAGAATTTCCCTTTGTTTTCTAGTTTTACAAGCATGAATCCTTTGGTTAGTTTTTAAAAATAAAATAAAAAATACACACATGTGAAAGTTATGCAATAACGATTTTAAAAATGAAACTCTATTGAGAGGTCTAAATTAATTCAATTATTAATTAATTTATAATTTTTATTGACACAATCCTACAAGTTTAGACTTTTATTCTGGTTGTTTTTATACTTTCAATTTTTGTTCAATTTAGTCTCTAGATTTTTTAAGAATTAAAATATTGATGTCGATATCAATAGTGAGATTTCAATTTTACGAATAACAATAAAATATTGATGTCGACGGATATTTCTATAAGTCAAAAGAATTATAAAAATTATTTAGATTAATAATTAAGTAGTTTTACTTTTTACTTTCAAATTAAGCTATGGATATTTTTATTAGTATATTTCTTATCATGTTGTACTAAATAGATGTATTTTTTTATGTTTTATGAAGATATCATTGAATATTGAATATTGATGTCTAACCCTTCTCAAAAATAGGTGAATCTAATACAATCCATACAGAAGCACAATGTTATCACATACCAAACGACAAGAGACATGCATATTAAAAAAGTAGAGACAGAGAAACACACAAAGATTGGTAACTTAGTTTGGTGTAAGATCGCCTACTCTAGGGGTAGTGTGATCGATGGAAAGAAACTTCATTCTTAAAGAGAGTTAAGTAATTATAACAAAGTATTTATCTGTTAGACTCAATGACTCACGTACAACTAACTTCGTAGTTTCAACTTAAGCTTCCCTGATCTTCTTACTTCTACAGAAGCAAACTTGATATTTTAATCCTTGTTATTTCGGTCTATATATATATTAGCTTTATGCTCTTAATAACCCAAAAACAAATTACATACACAACGAAAAAAATTTTAAAATGGGAATTATTAAATGCTTACCCTCAACCAAACAGGGAAGATCTACACGAGTTTACGGTAAAATTAATGGAGCTAATTGAATTAGTGCTGATAGCCATGGCAAGATCCTTAAACGTGGATCCGAATAGCTTTACAGAGCAGGTGGGGAATCGCCCAACTTTAATGACAAGGTTCAATTTCTATCCGCCGTGTTCGACGCCCCATCTTGTTCTTGGACTTAAAGAACACTCCGATGGATCAGCTATCACCATTATTTTACTCGACAAACAAGTCGAAGGGTTCGAATTGCGAAAGGATGATCAGTGGTACAGAGTCCCTGTTCCTACCATTGCTGATTCTCTTCTCATCAACATTGGGGAACAAGCCGAGGTAACAATGTTACACACTACTTTTTGAGTTTTGAATTTTGATTTTGCATCTTGGGTTTTTAAAGGATTATTGTTCTATGTCATGTGTGTGGAGATTATGAGCAATGGGATCTTCAAGAGCCCTGTTCATCGGGCGGTGACAAATTCAGAGAGGCAGAGGATTTCAGTGGCATGTTTCTGCTGCCCAGAAAAAGAAATAGAGATTAAGCCGATCGAGGGGCTGATCGACGAGAAGAGACCAAGGATGTTCAGAAGTGTGAAAGACTATTTGCAAACTTATTTTCTAAACTACCAGACGGGAGAGAGATCAGTTGATGGATTGAGGATTTAGAGTCTCCTTGTTTCTGTCGTGAACTCTGTGTTTTCATGGTGTGTTTGTAAAGATAGAACATATAACAAGTTCTACTGGTCAGAAGTTTTGGGTTCTTTTGAGCTTTTATAACACACACCTCTGTTTCAGAAAATGTTTTAAATTGTAGTTTTAAGTCTTTGTTTCCAAAAACTAGATTTGGTTTGGCCTTGTAAATCTTAATGATTAATATGTACTTGGAAGTTATTATTCAATTAAAATGTCAAGTGAACACTGAATTCAAACAA

mRNA sequence

GAATTCTCTTACTCTTAATTATTAAAAAAAATGATTGGTTTCAGTTTTGGTTTATTTATGGCACTTTCTTTAAGTATCTCTATCTACATCCATGGAATCCCATCGACACAAACATGGCAGAAAATTCTGACCTCACACAAACACTCCAAGAAAAGCTCCTCATCAACGGCGGCGATACGCCGGAAAGTTATATTTACAAAGGCGGCTACTGCGGTGGAGATTCCAACAATAATATCAATCCGCTTCCATTAGCAGAGATTCCGGTCGTTGACTGTTCTCGACTTTTATCGACGCCGGCGGGCGAGGCTCCGTTGAACGAGCTCCGGTTGGCTCTCAGTACTTGGGGATGTTTTCAGGCGATTAATCACAATATTTCAAGTTCATTTTTGGAGAAGATGAGAAAATTAAGCGAGCAATTTTTCTCACTGCCTATTGAAGAGAAGAAGAGATGTAGAAGAGAAGTTGATGGAATGGAAGGATATGGAAATGATTTGATTTTATCAAACCAACAAATTTTAGATTGGACTGATCGGTTGTATCTTATAGTAAATCCTCAGGATCAAAGAAAACTCAACTTTTGGCCTCTAAATCCTCAGTCTTTCAGGGAAGATCTACACGAGTTTACGGTAAAATTAATGGAGCTAATTGAATTAGTGCTGATAGCCATGGCAAGATCCTTAAACGTGGATCCGAATAGCTTTACAGAGCAGGTGGGGAATCGCCCAACTTTAATGACAAGGTTCAATTTCTATCCGCCGTGTTCGACGCCCCATCTTGTTCTTGGACTTAAAGAACACTCCGATGGATCAGCTATCACCATTATTTTACTCGACAAACAAGTCGAAGGGTTCGAATTGCGAAAGGATGATCAGTGGTACAGAGTCCCTGTTCCTACCATTGCTGATTCTCTTCTCATCAACATTGGGGAACAAGCCGAGATTATGAGCAATGGGATCTTCAAGAGCCCTGTTCATCGGGCGGTGACAAATTCAGAGAGGCAGAGGATTTCAGTGGCATGTTTCTGCTGCCCAGAAAAAGAAATAGAGATTAAGCCGATCGAGGGGCTGATCGACGAGAAGAGACCAAGGATGTTCAGAAGTGTGAAAGACTATTTGCAAACTTATTTTCTAAACTACCAGACGGGAGAGAGATCAGTTGATGGATTGAGGATTTAGAGTCTCCTTGTTTCTGTCGTGAACTCTGTGTTTTCATGGTGTGTTTGTAAAGATAGAACATATAACAAGTTCTACTGGTCAGAAGTTTTGGGTTCTTTTGAGCTTTTATAACACACACCTCTGTTTCAGAAAATGTTTTAAATTGTAGTTTTAAGTCTTTGTTTCCAAAAACTAGATTTGGTTTGGCCTTGTAAATCTTAATGATTAATATGTACTTGGAAGTTATTATTCAATTAAAATGTCAAGTGAACACTGAATTCAAACAA

Coding sequence (CDS)

ATGGCAGAAAATTCTGACCTCACACAAACACTCCAAGAAAAGCTCCTCATCAACGGCGGCGATACGCCGGAAAGTTATATTTACAAAGGCGGCTACTGCGGTGGAGATTCCAACAATAATATCAATCCGCTTCCATTAGCAGAGATTCCGGTCGTTGACTGTTCTCGACTTTTATCGACGCCGGCGGGCGAGGCTCCGTTGAACGAGCTCCGGTTGGCTCTCAGTACTTGGGGATGTTTTCAGGCGATTAATCACAATATTTCAAGTTCATTTTTGGAGAAGATGAGAAAATTAAGCGAGCAATTTTTCTCACTGCCTATTGAAGAGAAGAAGAGATGTAGAAGAGAAGTTGATGGAATGGAAGGATATGGAAATGATTTGATTTTATCAAACCAACAAATTTTAGATTGGACTGATCGGTTGTATCTTATAGTAAATCCTCAGGATCAAAGAAAACTCAACTTTTGGCCTCTAAATCCTCAGTCTTTCAGGGAAGATCTACACGAGTTTACGGTAAAATTAATGGAGCTAATTGAATTAGTGCTGATAGCCATGGCAAGATCCTTAAACGTGGATCCGAATAGCTTTACAGAGCAGGTGGGGAATCGCCCAACTTTAATGACAAGGTTCAATTTCTATCCGCCGTGTTCGACGCCCCATCTTGTTCTTGGACTTAAAGAACACTCCGATGGATCAGCTATCACCATTATTTTACTCGACAAACAAGTCGAAGGGTTCGAATTGCGAAAGGATGATCAGTGGTACAGAGTCCCTGTTCCTACCATTGCTGATTCTCTTCTCATCAACATTGGGGAACAAGCCGAGATTATGAGCAATGGGATCTTCAAGAGCCCTGTTCATCGGGCGGTGACAAATTCAGAGAGGCAGAGGATTTCAGTGGCATGTTTCTGCTGCCCAGAAAAAGAAATAGAGATTAAGCCGATCGAGGGGCTGATCGACGAGAAGAGACCAAGGATGTTCAGAAGTGTGAAAGACTATTTGCAAACTTATTTTCTAAACTACCAGACGGGAGAGAGATCAGTTGATGGATTGAGGATTTAG

Protein sequence

MAENSDLTQTLQEKLLINGGDTPESYIYKGGYCGGDSNNNINPLPLAEIPVVDCSRLLSTPAGEAPLNELRLALSTWGCFQAINHNISSSFLEKMRKLSEQFFSLPIEEKKRCRREVDGMEGYGNDLILSNQQILDWTDRLYLIVNPQDQRKLNFWPLNPQSFREDLHEFTVKLMELIELVLIAMARSLNVDPNSFTEQVGNRPTLMTRFNFYPPCSTPHLVLGLKEHSDGSAITIILLDKQVEGFELRKDDQWYRVPVPTIADSLLINIGEQAEIMSNGIFKSPVHRAVTNSERQRISVACFCCPEKEIEIKPIEGLIDEKRPRMFRSVKDYLQTYFLNYQTGERSVDGLRI

Homology

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BLAST of Lsi06G010220 vs. Swiss-Prot
Match: SRG1_ARATH (Protein SRG1 OS=Arabidopsis thaliana GN=SRG1 PE=2 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 2.5e-56
Identity = 106/309 (34.30%), Postives = 183/309 (59.22%), Query Frame = 1


Query: 48  EIPVVDCSRLLSTPAGEAPLNELRLALSTWGCFQAINHNISSSFLEKMRKLSEQFFSLPI 107
           EIP++D  RL S+   ++ + +L  A   WG FQ +NH I SSFL+K++   + FF+LP+
Sbjct: 52  EIPIIDMKRLCSSTTMDSEVEKLDFACKEWGFFQLVNHGIDSSFLDKVKSEIQDFFNLPM 111

Query: 108 EEKKRCRREVDGMEGYGNDLILSNQQILDWTDRLYLIVNPQDQRKLNFWPLNPQSFREDL 167
           EEKK+  +  D +EG+G   ++S  Q LDW D  +  V P + RK + +P  P  FR+ L
Sbjct: 112 EEKKKFWQRPDEIEGFGQAFVVSEDQKLDWADLFFHTVQPVELRKPHLFPKLPLPFRDTL 171

Query: 168 HEFTVKLMELIELVLIAMARSLNVDPNSFTEQVGNRPTLMT-RFNFYPPCSTPHLVLGLK 227
             ++ ++  + ++++  MAR+L + P    +   +  ++ + R N+YPPC  P  V+GL 
Sbjct: 172 EMYSSEVQSVAKILIAKMARALEIKPEELEKLFDDVDSVQSMRMNYYPPCPQPDQVIGLT 231

Query: 228 EHSDGSAITIILLDKQVEGFELRKDDQWYRVPVPTIADSLLINIGEQAEIMSNGIFKSPV 287
            HSD   +T+++    VEG +++KD +W  VPV  + ++ ++NIG+  EI++NG ++S  
Sbjct: 232 PHSDSVGLTVLMQVNDVEGLQIKKDGKW--VPVKPLPNAFIVNIGDVLEIITNGTYRSIE 291

Query: 288 HRAVTNSERQRISVACFCCPEKEIEIKPIEGLIDEKRPRMFR--SVKDYLQTYFLNYQTG 347
           HR V NSE++R+S+A F       E+ P + L++ ++   F+  ++K+Y    F     G
Sbjct: 292 HRGVVNSEKERLSIATFHNVGMYKEVGPAKSLVERQKVARFKRLTMKEYNDGLFSRTLDG 351

Query: 348 ERSVDGLRI 354
           +  +D LRI
Sbjct: 352 KAYLDALRI 358

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BLAST of Lsi06G010220 vs. Swiss-Prot
Match: NCS1_COPJA (S-norcoclaurine synthase 1 OS=Coptis japonica GN=NCS1 PE=1 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 1.4e-51
Identity = 103/274 (37.59%), Postives = 159/274 (58.03%), Query Frame = 1


Query: 48  EIPVVDCSRLLSTPAGEAPLNELRLALSTWGCFQAINHNISSSFLEKMRKLSEQFFSLPI 107
           EIPV+D SRLL        L +   A   WG FQ INH +    +EKM+  +E FF LP 
Sbjct: 50  EIPVIDLSRLLDQQYACDELAKFHSACLDWGFFQLINHGVREEVIEKMKVDTEDFFRLPF 109

Query: 108 EEKKRCRREVDGMEGYGNDLILSNQQILDWTDRLYLIVNPQDQRKLNFWPLNPQSFREDL 167
           +EK   R+  +GMEGYG   + S +Q LDW D  +LI  P  +R + FWP +P SFRE +
Sbjct: 110 KEKNAYRQLPNGMEGYGQAFVTSEEQKLDWADMHFLITKPVQERNMRFWPTSPTSFRETM 169

Query: 168 HEFTVKLMELIELVLIAMARSLNVDPNSFTEQVGNRPTLMTRFNFYPPCSTPHLVLGLKE 227
            +++++L ++   +   MA++L ++    T+ +  R          P  S+    LGL  
Sbjct: 170 EKYSMELQKVAMCLTGMMAKNLGLESEILTKPL--RTVFNREDELLPSMSSCGEGLGLSP 229

Query: 228 HSDGSAITIILLDKQVEGFELRKDDQWYRVPVPTIADSLLINIGEQAEIMSNGIFKSPVH 287
           HSD + +T+++   +V G  ++KD++W  VP+  I  + ++NIG+  EIMSNGI+KS  H
Sbjct: 230 HSDATGLTLLIQVNEVNGLHIKKDEKW--VPIKPILGAFVVNIGDVIEIMSNGIYKSIEH 289

Query: 288 RAVTNSERQRISVACFCCPEKEIEIKPIEGLIDE 322
           RAV N++++R+S+A F  PE   +I P+  L+ E
Sbjct: 290 RAVINTDKERLSIAAFHDPEYGTKIGPLPDLVKE 319

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BLAST of Lsi06G010220 vs. Swiss-Prot
Match: DIOX3_PAPSO (Codeine O-demethylase OS=Papaver somniferum GN=CODM PE=1 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 2.7e-50
Identity = 117/352 (33.24%), Postives = 191/352 (54.26%), Query Frame = 1


Query: 10  TLQEKLLINGGDTPESYIYKGGYCGGDSN-NNINPLPLAE--IPVVDCSRLLSTP--AGE 69
           ++QE   +   + P  Y      C G+S  NNI      +  +PV+D   LLS     G+
Sbjct: 17  SVQELAKLTLAEIPSRYT-----CTGESPLNNIGASVTDDETVPVIDLQNLLSPEPVVGK 76

Query: 70  APLNELRLALSTWGCFQAINHNISSSFLEKMRKLSEQFFSLPIEEKKRCRREVDGMEGYG 129
             L++L  A   WG FQ +NH + +  ++ ++   + FF+LP+ EK +  ++    EG+G
Sbjct: 77  LELDKLHSACKEWGFFQLVNHGVDALLMDNIKSEIKGFFNLPMNEKTKYGQQDGDFEGFG 136

Query: 130 NDLILSNQQILDWTDRLYLIVNPQDQRKLNFWPLNPQSFREDLHEFTVKLMELIELVLIA 189
              I S  Q LDWT+   ++  P   RK + +P  P  FRE L  +  K+ +L  +V   
Sbjct: 137 QPYIESEDQRLDWTEVFSMLSLPLHLRKPHLFPELPLPFRETLESYLSKMKKLSTVVFEM 196

Query: 190 MARSLN-VDPNSFTEQVGNRPTLMTRFNFYPPCSTPHLVLGLKEHSDGSAITIILLDKQV 249
           + +SL  V+    T+   +    M R N+YPPC  P LVLGL  HSD S +TI+L   +V
Sbjct: 197 LEKSLQLVEIKGMTDLFEDGLQTM-RMNYYPPCPRPELVLGLTSHSDFSGLTILLQLNEV 256

Query: 250 EGFELRKDDQWYRVPVPTIADSLLINIGEQAEIMSNGIFKSPVHRAVTNSERQRISVACF 309
           EG ++RK+++W  + +  + D+ ++N+G+  EIM+NGI++S  HRAV NS ++R+S+A F
Sbjct: 257 EGLQIRKEERW--ISIKPLPDAFIVNVGDILEIMTNGIYRSVEHRAVVNSTKERLSIATF 316

Query: 310 CCPEKEIEIKPIEGLIDEKRPRMFR--SVKDYLQTYFLNYQTGERSVDGLRI 354
              + E EI PI  L+  + P +F+    +D L+        G+  +D +R+
Sbjct: 317 HDSKLESEIGPISSLVTPETPALFKRGRYEDILKENLSRKLDGKSFLDYMRM 360

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BLAST of Lsi06G010220 vs. Swiss-Prot
Match: DIOX2_PAPSO (Probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Papaver somniferum GN=DIOX2 PE=2 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 1.8e-46
Identity = 99/312 (31.73%), Postives = 170/312 (54.49%), Query Frame = 1


Query: 49  IPVVDCSRLLST-PAGEA-PLNELRLALSTWGCFQAINHNISSSFLEKMRKLSEQFFSLP 108
           +PV+D   L+S+ P  E   L+ L  A   WG FQ +NH + +S ++ ++   + FF+L 
Sbjct: 55  VPVIDIENLISSEPVTEKLELDRLHSACKEWGFFQVVNHGVDTSLVDNVKSDIQGFFNLS 114

Query: 109 IEEKKRCRREVDGMEGYGNDLILSNQQILDWTDRLYLIVNPQDQRKLNFWPLNPQSFRED 168
           + EK +  ++   +EG+G   + S  Q LDW D   ++  P   RK + +   P   RE 
Sbjct: 115 MNEKIKYGQKDGDVEGFGQAFVASEDQTLDWADIFMILTLPLHLRKPHLFSKLPLPLRET 174

Query: 169 LHEFTVKLMELIELVLIAMARSLNVDPNSFTE--QVGNRPTLMTRFNFYPPCSTPHLVLG 228
           +  ++ ++ +L  ++   M ++L V      E  +V    T + R N+YPPC  P L +G
Sbjct: 175 IESYSSEMKKLSMVLFEKMEKALQVQAVEIKEISEVFKDMTQVMRMNYYPPCPQPELAIG 234

Query: 229 LKEHSDGSAITIILLDKQVEGFELRKDDQWYRVPVPTIADSLLINIGEQAEIMSNGIFKS 288
           L  HSD   +TI+L   +VEG +++ + +W  + V  + ++ ++N+G+  EIM+NG+++S
Sbjct: 235 LTPHSDFGGLTILLQLNEVEGLQIKNEGRW--ISVKPLPNAFVVNVGDVLEIMTNGMYRS 294

Query: 289 PVHRAVTNSERQRISVACFCCPEKEIEIKPIEGLIDEKRPRMFRSVKDY---LQTYFLNY 348
             HRAV NS ++R+S+A F  P  E EI PI  LI    P +FRS   Y   ++ +    
Sbjct: 295 VDHRAVVNSTKERLSIATFHDPNLESEIGPISSLITPNTPALFRSGSTYGELVEEFHSRK 354

Query: 349 QTGERSVDGLRI 354
             G+  +D +R+
Sbjct: 355 LDGKSFLDSMRM 364

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BLAST of Lsi06G010220 vs. Swiss-Prot
Match: DIOX1_PAPSO (Thebaine 6-O-demethylase OS=Papaver somniferum GN=T6ODM PE=1 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 9.0e-46
Identity = 97/289 (33.56%), Postives = 156/289 (53.98%), Query Frame = 1


Query: 49  IPVVDCSRLLSTPA--GEAPLNELRLALSTWGCFQAINHNISSSFLEKMRKLSEQFFSLP 108
           IPV+D   LLS     G+  L+ L  A   WG FQ +NH + +S ++ ++   + FF+L 
Sbjct: 55  IPVIDIENLLSPEPIIGKLELDRLHFACKEWGFFQVVNHGVDASLVDSVKSEIQGFFNLS 114

Query: 109 IEEKKRCRREVDGMEGYGNDLILSNQQILDWTDRLYLIVNPQDQRKLNFWPLNPQSFRED 168
           ++EK +  +E   +EG+G   I S  Q LDW D   +   P   RK + +   P   RE 
Sbjct: 115 MDEKTKYEQEDGDVEGFGQGFIESEDQTLDWADIFMMFTLPLHLRKPHLFSKLPVPLRET 174

Query: 169 LHEFTVKLMELIELVLIAMARSLNVDPNSF--TEQVGNRPTLMTRFNFYPPCSTPHLVLG 228
           +  ++ ++ +L  ++   M ++L V         +V    T   R N+YPPC  P+L +G
Sbjct: 175 IESYSSEMKKLSMVLFNKMEKALQVQAAEIKGMSEVFIDGTQAMRMNYYPPCPQPNLAIG 234

Query: 229 LKEHSDGSAITIILLDKQVEGFELRKDDQWYRVPVPTIADSLLINIGEQAEIMSNGIFKS 288
           L  HSD   +TI+L   +VEG +++++  W  + V  + ++ ++N+G+  EIM+NGI+ S
Sbjct: 235 LTSHSDFGGLTILLQINEVEGLQIKREGTW--ISVKPLPNAFVVNVGDILEIMTNGIYHS 294

Query: 289 PVHRAVTNSERQRISVACFCCPEKEIEIKPIEGLIDEKRPRMFRSVKDY 334
             HRAV NS  +R+S+A F  P  E  I PI  LI  + P +F+S   Y
Sbjct: 295 VDHRAVVNSTNERLSIATFHDPSLESVIGPISSLITPETPALFKSGSTY 341

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BLAST of Lsi06G010220 vs. TrEMBL
Match: A0A0A0LW96_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G212830 PE=3 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 2.7e-145
Identity = 246/300 (82.00%), Postives = 275/300 (91.67%), Query Frame = 1


Query: 55  SRLLSTP-AGEAPLNELRLALSTWGCFQAINHNISSSFLEKMRKLSEQFFSLPIEEKKRC 114
           +R L +P AGE PLN+LRLALSTWGCFQA NH+ISSSFLEK+RK+SEQFFSLPIEEK R 
Sbjct: 278 NRYLKSPSAGEGPLNDLRLALSTWGCFQATNHSISSSFLEKLRKISEQFFSLPIEEKMRY 337

Query: 115 RREVDGMEGYGNDLILSNQQILDWTDRLYLIVNPQDQRKLNFWPLNPQSFREDLHEFTVK 174
            REVDGMEGYGNDL  SNQQ LDW+DRLY + +P+D+R+L+ WPLNP SFREDLHE+TVK
Sbjct: 338 GREVDGMEGYGNDLTFSNQQTLDWSDRLYFVTSPEDERRLDLWPLNPPSFREDLHEYTVK 397

Query: 175 LMELIELVLIAMARSLNVDPNSFTEQVGNRPTLMTRFNFYPPCSTPHLVLGLKEHSDGSA 234
           +ME+IE VLIAMARSLNV+PNSFT+QVG RPTL TRFNFYPPCSTPHLVLGLKEHSDGSA
Sbjct: 398 IMEIIETVLIAMARSLNVEPNSFTDQVGERPTLFTRFNFYPPCSTPHLVLGLKEHSDGSA 457

Query: 235 ITIILLDKQVEGFELRKDDQWYRVPVPTIADSLLINIGEQAEIMSNGIFKSPVHRAVTNS 294
           ITI+LLDKQVEG +LRKDDQWYRVPVP IADSLL+ IGEQAE+MSNGIFKS VHRAVTNS
Sbjct: 458 ITILLLDKQVEGLQLRKDDQWYRVPVPAIADSLLLVIGEQAEVMSNGIFKSSVHRAVTNS 517

Query: 295 ERQRISVACFCCPEKEIEIKPIEGLIDEKRPRMFRSVKDYLQTYFLNYQTGERSVDGLRI 354
           ERQRISV CFCCPEK+IEIKP+EGLIDEKRPR+FRSVK+YL+TYF NYQ G+R+VDGLRI
Sbjct: 518 ERQRISVVCFCCPEKDIEIKPVEGLIDEKRPRLFRSVKNYLETYFQNYQEGQRTVDGLRI 577

[Close]

BLAST of Lsi06G010220 vs. TrEMBL
Match: U7E2X6_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0025s00430g PE=3 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 6.4e-107
Identity = 183/353 (51.84%), Postives = 252/353 (71.39%), Query Frame = 1


Query: 1   MAENSDLTQTLQEKLLINGGDTPESYIYKGGYCGGDSNNNINPLPLAEIPVVDCSRLLST 60
           MA  ++L     +++  NG + P  Y  KG     D+     P+PL EIPVVD   L S 
Sbjct: 1   MAAMAELISNSVQEMATNGQEPPVKYFSKGN----DAGVLDAPVPLIEIPVVDLGLLTSP 60

Query: 61  PAGEAPLNELRLALSTWGCFQAINHNISSSFLEKMRKLSEQFFSLPIEEKKRCRREVDGM 120
                 L +L+LALS+WGCFQ INH ++SSFL+K+R++S+QFF  P+EEK++  RE D +
Sbjct: 61  LTSAQELEKLKLALSSWGCFQVINHGMTSSFLDKIREVSKQFFGFPMEEKQKYSREADSI 120

Query: 121 EGYGNDLILSNQQILDWTDRLYLIVNPQDQRKLNFWPLNPQSFREDLHEFTVKLMELIEL 180
           EGYGND+ILS+ Q +DWTDRLYL ++P+DQRK+ FWP NP+ FRE LHE+TVKL E  + 
Sbjct: 121 EGYGNDMILSDHQTVDWTDRLYLTISPEDQRKIKFWPENPKDFRETLHEYTVKLQETNDF 180

Query: 181 VLIAMARSLNVDPNSFTEQVGNRPTLMTRFNFYPPCSTPHLVLGLKEHSDGSAITIILLD 240
           +L AMARSLN++ + F +Q G +P +  RFNFYPPC  P  +LG+K H+D SA+T +L D
Sbjct: 181 LLRAMARSLNLEESCFLDQYGEQPLVTARFNFYPPCPRPDRILGVKPHADASAVTFLLQD 240

Query: 241 KQVEGFELRKDDQWYRVPVPTIADSLLINIGEQAEIMSNGIFKSPVHRAVTNSERQRISV 300
           K+VEG +  KD++W+RVP+  I  +LL+N+G+Q EIMSNGIFKSPVHR VTN+ER+R ++
Sbjct: 241 KEVEGLQFLKDNEWFRVPI--IPHALLVNVGDQVEIMSNGIFKSPVHRVVTNTERERNTL 300

Query: 301 ACFCCPEKEIEIKPIEGLIDEKRPRMFRSVKDYLQTYFLNYQTGERSVDGLRI 354
           A FC PE + EIKP + LI E RP +++ VKDY+  YF  YQ G+R ++ ++I
Sbjct: 301 AVFCIPESDNEIKPADQLISETRPSLYKKVKDYVSIYFQYYQQGKRPIEAVKI 347

[Close]

BLAST of Lsi06G010220 vs. TrEMBL
Match: B9N703_POPTR (Oxidoreductase family protein OS=Populus trichocarpa GN=POPTR_0006s06130g PE=3 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 1.5e-103
Identity = 177/353 (50.14%), Postives = 251/353 (71.10%), Query Frame = 1


Query: 1   MAENSDLTQTLQEKLLINGGDTPESYIYKGGYCGGDSNNNINPLPLAEIPVVDCSRLLST 60
           MA  + L     +++ +NG + P  Y  KG   G    +    +PL +IPVVD   L S 
Sbjct: 1   MAAKTVLISNSVQEMALNGEEPPVKYFCKGNDVGVLDAS----VPLIDIPVVDLGLLTSP 60

Query: 61  PAGEAPLNELRLALSTWGCFQAINHNISSSFLEKMRKLSEQFFSLPIEEKKRCRREVDGM 120
                 L +  LA S+WGCFQ +NH ++SSFL+K+R +S++FF+L +E+K++  RE D +
Sbjct: 61  STSAQELEKFHLAASSWGCFQVVNHGMTSSFLDKIRDVSKRFFALSMEDKQKYSREADSI 120

Query: 121 EGYGNDLILSNQQILDWTDRLYLIVNPQDQRKLNFWPLNPQSFREDLHEFTVKLMELIEL 180
           EGYGND+ILS+ Q +DW+DRLYL ++P+DQRK+ FWP NP+ FRE L+E+T+KL E+ E+
Sbjct: 121 EGYGNDMILSDHQTIDWSDRLYLTISPEDQRKIKFWPENPKDFRETLNEYTMKLQEINEI 180

Query: 181 VLIAMARSLNVDPNSFTEQVGNRPTLMTRFNFYPPCSTPHLVLGLKEHSDGSAITIILLD 240
           +L AMA SLN++ +SF +Q G RP +  RFNFYPPC  P  +LG+K H+D SAIT +L D
Sbjct: 181 LLRAMAMSLNLEESSFLDQYGERPLVAARFNFYPPCPRPDRILGVKPHADASAITFLLQD 240

Query: 241 KQVEGFELRKDDQWYRVPVPTIADSLLINIGEQAEIMSNGIFKSPVHRAVTNSERQRISV 300
           K+VEG +  KD+QW+RVP+  I  +LLIN+G+Q EIMSNGIFKSPVHR VTN+E++R ++
Sbjct: 241 KEVEGLQFLKDNQWFRVPI--IPHALLINVGDQVEIMSNGIFKSPVHRVVTNTEKERNTL 300

Query: 301 ACFCCPEKEIEIKPIEGLIDEKRPRMFRSVKDYLQTYFLNYQTGERSVDGLRI 354
           A FC P+ + EIKP +GLI E RP +++ VKDY+  YF  YQ G+R ++ ++I
Sbjct: 301 AVFCIPDSDKEIKPADGLISETRPSLYKKVKDYVSIYFQYYQQGKRPIEAVKI 347

[Close]

BLAST of Lsi06G010220 vs. TrEMBL
Match: A0A059CLG0_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_C00494 PE=3 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 5.6e-103
Identity = 178/348 (51.15%), Postives = 253/348 (72.70%), Query Frame = 1


Query: 8   TQTLQEKLLINGGDTPESYIYKGGYCGGDSNNNINPLPLAEIPVVDCSRLLSTPAGEA-- 67
           +Q++QE L+++GG  PE Y YK         +    LPL ++P +D   L ++ +  A  
Sbjct: 12  SQSVQE-LILDGGTVPERYAYKDDKTASFDES----LPLTDVPAIDLGLLTASSSSSAGE 71

Query: 68  PLNELRLALSTWGCFQAINHNISSSFLEKMRKLSEQFFSLPIEEKKRCRREVDGMEGYGN 127
            L +LR AL+ WGCF AINH IS SFL+++ +L++QFF+LP EEK++C R  DG+EGYGN
Sbjct: 72  ELIKLRSALTCWGCFHAINHGISCSFLDQLHELTKQFFALPKEEKEKCARAPDGIEGYGN 131

Query: 128 DLILSNQQILDWTDRLYLIVNPQDQRKLNFWPLNPQSFREDLHEFTVKLMELIELVLIAM 187
           D++ S QQ++DW DR+Y+I++P+D+RKL  WP  P++FRE +HE++V L  +I  V  AM
Sbjct: 132 DMVFSEQQVIDWNDRVYIIISPEDKRKLEVWPEKPETFREVIHEYSVMLRPVINNVFKAM 191

Query: 188 ARSLNVDPNSFTEQVGNRPTLMTRFNFYPPCSTPHLVLGLKEHSDGSAITIILLDKQVEG 247
           A SLN++ N F  Q G R  +  RFN+YP C  P LVLGLK H+DGSAIT++L D++VEG
Sbjct: 192 ALSLNLEENCFLNQFGERGVMFARFNYYPRCPRPDLVLGLKPHADGSAITVVLPDREVEG 251

Query: 248 FELRKDDQWYRVPVPTIADSLLINIGEQAEIMSNGIFKSPVHRAVTNSERQRISVACFCC 307
            + ++D QW+   VPTI ++LLIN+G+Q EIMSNGIFKSPVHRAVTN+ER RIS+A FC 
Sbjct: 252 LQFQRDGQWF--TVPTIPNALLINVGDQVEIMSNGIFKSPVHRAVTNAERDRISLAVFCT 311

Query: 308 PEKEIEIKPIEGLIDEKRPRMFRSVKDYLQTYFLNYQTGERSVDGLRI 354
            E+E EI P +GLI+E+ PR+++ +++Y +TYF  YQ G+RS+D ++I
Sbjct: 312 SEEENEIGPADGLINEETPRLYKKIRNYPETYFEYYQLGKRSIDAVKI 352

[Close]

BLAST of Lsi06G010220 vs. TrEMBL
Match: U7E2D8_POPTR (Oxidoreductase family protein OS=Populus trichocarpa GN=POPTR_0025s00440g PE=3 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 4.7e-102
Identity = 175/341 (51.32%), Postives = 244/341 (71.55%), Query Frame = 1


Query: 13  EKLLINGGDTPESYIYKGGYCGGDSNNNINPLPLAEIPVVDCSRLLSTPAGEAPLNELRL 72
           ++L++N  + P +Y Y+ G  G    +    LP+ E+PV+D SRL S       + +L  
Sbjct: 18  QELVMNNEEPPGNYFYEDGVNGVLDRS----LPVLEMPVIDISRLTSPSTSREEVEKLHS 77

Query: 73  ALSTWGCFQAINHNISSSFLEKMRKLSEQFFSLPIEEKKRCRREVDGMEGYGNDLILSNQ 132
           AL + GCF +INH I+  FL+++R ++ QFF+LP+EEK +  R VD  EGYGND+ILS  
Sbjct: 78  ALISCGCFMSINHGITGVFLDQVRSVTAQFFALPMEEKLKYSRAVDSTEGYGNDMILSED 137

Query: 133 QILDWTDRLYLIVNPQDQRKLNFWPLNPQSFREDLHEFTVKLMELIELVLIAMARSLNVD 192
           QILDWTDRLYLIV+P+DQR+  FWP  P+ FRE L E+T KL  ++E+VL AMARSLN++
Sbjct: 138 QILDWTDRLYLIVSPEDQRQFKFWPEKPEIFREILQEYTTKLKVIVEVVLKAMARSLNLE 197

Query: 193 PNSFTEQVGNRPTLMTRFNFYPPCSTPHLVLGLKEHSDGSAITIILLDKQVEGFELRKDD 252
            N F ++ G R  +  RFNF+PPC  P   LGLK H+DGSAITI+L DK+VEG +  KDD
Sbjct: 198 DNCFLDKYGERALMQARFNFFPPCPRPDRSLGLKPHADGSAITIVLQDKEVEGLQFLKDD 257

Query: 253 QWYRVPVPTIADSLLINIGEQAEIMSNGIFKSPVHRAVTNSERQRISVACFCCPEKEIEI 312
           QW+RVP+  +  +LLIN+G+Q+E+MSNG FKSPVHR VTNSER+R SVA FC P+ + +I
Sbjct: 258 QWFRVPI-QLPHALLINVGDQSEVMSNGFFKSPVHRVVTNSERERTSVAVFCSPDPDNDI 317

Query: 313 KPIEGLIDEKRPRMFRSVKDYLQTYFLNYQTGERSVDGLRI 354
           +P++G + E RPR+++ V+DY+  YF  YQ G+R ++ ++I
Sbjct: 318 EPVDGAVSETRPRLYKKVQDYVSKYFQYYQEGKRPIEAVKI 353

[Close]

BLAST of Lsi06G010220 vs. TAIR10
Match: AT5G20400.1 (AT5G20400.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 357.8 bits (917), Expect = 7.5e-99
Identity = 171/350 (48.86%), Postives = 243/350 (69.43%), Query Frame = 1


Query: 9   QTLQEKLLINGGDTPESYIYKGGYCGGDSNNNINPL----PLAEIPVVDCSRLLSTP-AG 68
           +T+QE ++  G   PE Y++         +  + PL    P  +IP +D + LLS+  AG
Sbjct: 7   KTVQE-VVAAGEGLPERYLH-----APTGDGEVQPLNAAVPEMDIPAIDLNLLLSSSEAG 66

Query: 69  EAPLNELRLALSTWGCFQAINHNISSSFLEKMRKLSEQFFSLPIEEKKRCRREVDGMEGY 128
           +  L++L  ALSTWG  Q +NH I+ +FL+K+ KL+++FF+LP EEK++C RE+D ++GY
Sbjct: 67  QQELSKLHSALSTWGVVQVMNHGITKAFLDKIYKLTKEFFALPTEEKQKCAREIDSIQGY 126

Query: 129 GNDLILSNQQILDWTDRLYLIVNPQDQRKLNFWPLNPQSFREDLHEFTVKLMELIELVLI 188
           GND+IL + Q+LDW DRLY+   P+DQR+LNFWP  P  FRE LHE+T+K   +IE    
Sbjct: 127 GNDMILWDDQVLDWIDRLYITTYPEDQRQLNFWPEVPLGFRETLHEYTMKQRIVIEQFFK 186

Query: 189 AMARSLNVDPNSFTEQVGNRPTLMTRFNFYPPCSTPHLVLGLKEHSDGSAITIILLDKQV 248
           AMARSL ++ NSF +  G   TL TRFN YPPC +P  V+G+K H+DGSAIT++L DK V
Sbjct: 187 AMARSLELEENSFLDMYGESATLDTRFNMYPPCPSPDKVIGVKPHADGSAITLLLPDKDV 246

Query: 249 EGFELRKDDQWYRVPVPTIADSLLINIGEQAEIMSNGIFKSPVHRAVTNSERQRISVACF 308
            G + +KD +WY+ P+  + D++LIN+G+Q EIMSNGI+KSPVHR VTN E++RISVA F
Sbjct: 247 GGLQFQKDGKWYKAPI--VPDTILINVGDQMEIMSNGIYKSPVHRVVTNREKERISVATF 306

Query: 309 CCPEKEIEIKPIEGLIDEKRPRMFRSVKDYLQTYFLNYQTGERSVDGLRI 354
           C P  + EI+P+  L+ E RPR++++VK Y++ YF  YQ G R ++   I
Sbjct: 307 CIPGADKEIQPVNELVSEARPRLYKTVKKYVELYFKYYQQGRRPIEAALI 348

[Close]

BLAST of Lsi06G010220 vs. TAIR10
Match: AT1G49390.1 (AT1G49390.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 337.0 bits (863), Expect = 1.4e-92
Identity = 162/346 (46.82%), Postives = 231/346 (66.76%), Query Frame = 1


Query: 9   QTLQEKLLINGGDTPESYIYKGGYCGGDSNNNINPLPLAEIPVVDCSRLLSTPA-GEAPL 68
           +T+QE ++  G   PE Y++      G+S      +P  +IP +D S L S+   G+  +
Sbjct: 7   KTVQE-VVAAGQGLPERYLH-APTGEGESQPLNGAVPEMDIPAIDLSLLFSSSVDGQEEM 66

Query: 69  NELRLALSTWGCFQAINHNISSSFLEKMRKLSEQFFSLPIEEKKRCRREVDGMEGYGNDL 128
            +L  ALSTWG  Q +NH I+ +FL+K+ KL++QFF+LP EEK +C RE   ++GYGND+
Sbjct: 67  KKLHSALSTWGVVQVMNHGITEAFLDKIYKLTKQFFALPTEEKHKCARETGNIQGYGNDM 126

Query: 129 ILSNQQILDWTDRLYLIVNPQDQRKLNFWPLNPQSFREDLHEFTVKLMELIELVLIAMAR 188
           ILS+ Q+LDW DRL+L   P+D+R+L FWP  P  F E L E+T+K   LIE    AMAR
Sbjct: 127 ILSDNQVLDWIDRLFLTTYPEDKRQLKFWPQVPVGFSETLDEYTMKQRVLIEKFFKAMAR 186

Query: 189 SLNVDPNSFTEQVGNRPTLMTRFNFYPPCSTPHLVLGLKEHSDGSAITIILLDKQVEGFE 248
           SL ++ N F E  G    + +RFNF+PPC  P  V+G+K H+DGSAIT++L DK VEG +
Sbjct: 187 SLELEENCFLEMYGENAVMNSRFNFFPPCPRPDKVIGIKPHADGSAITLLLPDKDVEGLQ 246

Query: 249 LRKDDQWYRVPVPTIADSLLINIGEQAEIMSNGIFKSPVHRAVTNSERQRISVACFCCPE 308
             KD +WY+ P+  + D++LI +G+Q EIMSNGI+KSPVHR VTN E++RISVA FC P 
Sbjct: 247 FLKDGKWYKAPI--VPDTILITLGDQMEIMSNGIYKSPVHRVVTNREKERISVATFCVPG 306

Query: 309 KEIEIKPIEGLIDEKRPRMFRSVKDYLQTYFLNYQTGERSVDGLRI 354
            + EI P +GL+ E RPR++++V  Y+  ++  YQ G R+++   I
Sbjct: 307 LDKEIHPADGLVTEARPRLYKTVTKYVDLHYKYYQQGRRTIEAALI 348

[Close]

BLAST of Lsi06G010220 vs. TAIR10
Match: AT5G54000.1 (AT5G54000.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 330.5 bits (846), Expect = 1.3e-90
Identity = 160/346 (46.24%), Postives = 227/346 (65.61%), Query Frame = 1


Query: 9   QTLQEKLLINGGDTPESYIYKGGYCGGDSNNNINPLPLAEIPVVDCSRLLSTPA-GEAPL 68
           +T+QE ++  G   PE Y+Y     G         LP  +I ++D + L S+   G   L
Sbjct: 7   KTVQE-VVAAGEKLPERYLYTPTGDGEGDQPFNGLLPEMKISIIDLNLLFSSSDDGREEL 66

Query: 69  NELRLALSTWGCFQAINHNISSSFLEKMRKLSEQFFSLPIEEKKRCRREVDGMEGYGNDL 128
           ++L  A+STWG  Q +NH IS + L+K+ +L++QFF LP +EK++  RE+   +G+GND+
Sbjct: 67  SKLHSAISTWGVVQVMNHGISEALLDKIHELTKQFFVLPTKEKQKYAREISSFQGFGNDM 126

Query: 129 ILSNQQILDWTDRLYLIVNPQDQRKLNFWPLNPQSFREDLHEFTVKLMELIELVLIAMAR 188
           ILS+ Q+LDW DRLYLI  P+DQR+L FWP NP  FRE LHE+T+K   ++E    A+AR
Sbjct: 127 ILSDDQVLDWVDRLYLITYPEDQRQLKFWPENPSGFRETLHEYTMKQQLVVEKFFKALAR 186

Query: 189 SLNVDPNSFTEQVGNRPTLMTRFNFYPPCSTPHLVLGLKEHSDGSAITIILLDKQVEGFE 248
           SL ++ N F E  G   TL TRFN YPPC  P  VLGLK HSDGSA T+IL DK VEG +
Sbjct: 187 SLELEDNCFLEMHGENATLETRFNIYPPCPRPDKVLGLKPHSDGSAFTLILPDKNVEGLQ 246

Query: 249 LRKDDQWYRVPVPTIADSLLINIGEQAEIMSNGIFKSPVHRAVTNSERQRISVACFCCPE 308
             KD +WY+  +  +  ++LIN+G+  E+MSNGI+KSPVHR V N +++RI VA FC  +
Sbjct: 247 FLKDGKWYKASI--LPHTILINVGDTMEVMSNGIYKSPVHRVVLNGKKERIYVATFCNAD 306

Query: 309 KEIEIKPIEGLIDEKRPRMFRSVKDYLQTYFLNYQTGERSVDGLRI 354
           ++ EI+P+ GL+ E RPR++++VK   + +F  YQ G R ++   I
Sbjct: 307 EDKEIQPLNGLVSEARPRLYKAVKKSEKNFFDYYQQGRRPIEAAMI 349

[Close]

BLAST of Lsi06G010220 vs. TAIR10
Match: AT5G20550.1 (AT5G20550.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 325.5 bits (833), Expect = 4.1e-89
Identity = 159/342 (46.49%), Postives = 230/342 (67.25%), Query Frame = 1


Query: 9   QTLQEKLLINGGDTPESYIYKGGYCGGDSNNNINP-LPLAEIPVVDCSRLLS-TPAGEAP 68
           +T+QE ++  G   PE Y+        D+  ++N  +P+ +IP +D S LLS +  G   
Sbjct: 7   KTVQE-VVAAGEGIPERYLQPPAV--DDNGQHLNAAVPVMDIPAIDLSLLLSPSDDGREE 66

Query: 69  LNELRLALSTWGCFQAINHNISSSFLEKMRKLSEQFFSLPIEEKKRCRREVDGMEGYGND 128
           L++L  ALSTWG  Q INH I+ + L+K+ KL+++F +LP EEK++  RE+  ++GYGND
Sbjct: 67  LSKLHSALSTWGVVQVINHGITKALLDKIYKLTKEFCALPSEEKQKYAREIGSIQGYGND 126

Query: 129 LILSNQQILDWTDRLYLIVNPQDQRKLNFWPLNPQSFREDLHEFTVKLMELIELVLIAMA 188
           +IL + Q+LDW DRLY+   P+DQR+L FWP  P  FRE LHE+T+K   +   V  AMA
Sbjct: 127 MILWDDQVLDWIDRLYITTYPEDQRQLKFWPDVPVGFRETLHEYTMKQHLVFNQVFKAMA 186

Query: 189 RSLNVDPNSFTEQVGNRPTLMTRFNFYPPCSTPHLVLGLKEHSDGSAITIILLDKQVEGF 248
            SL ++ N F +  G   T+ TRFN YPPC  P  V+G++ H+D SA T++L DK VEG 
Sbjct: 187 ISLELEENCFLDMCGENATMDTRFNMYPPCPRPDKVIGVRPHADKSAFTLLLPDKNVEGL 246

Query: 249 ELRKDDQWYRVPVPTIADSLLINIGEQAEIMSNGIFKSPVHRAVTNSERQRISVACFCCP 308
           +  KD +WY+ PV   +D++LIN+G+Q EIMSNGI+KSPVHR VTN+E++RISVA FC P
Sbjct: 247 QFLKDGKWYKAPV-VASDTILINVGDQMEIMSNGIYKSPVHRVVTNTEKERISVATFCIP 306

Query: 309 EKEIEIKPIEGLIDEKRPRMFRSVKDYLQTYFLNYQTGERSV 349
             + EI+P++GL+ E RPR+++ VK+Y+      Y  G+R +
Sbjct: 307 GADKEIQPVDGLVSEARPRLYKPVKNYVDLLNKYYIQGQRPI 344

[Close]

BLAST of Lsi06G010220 vs. TAIR10
Match: AT1G78550.1 (AT1G78550.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 225.7 bits (574), Expect = 4.4e-59
Identity = 115/309 (37.22%), Postives = 183/309 (59.22%), Query Frame = 1


Query: 47  AEIPVVDCSRLLSTPAGEAPLNELRLALSTWGCFQAINHNISSSFLEKMRKLSEQFFSLP 106
           +EIPV+D +RL S  A ++ L +L  A   WG FQ +NH I SSFLEK+    ++FF+LP
Sbjct: 51  SEIPVIDMTRLCSVSAMDSELKKLDFACQDWGFFQLVNHGIDSSFLEKLETEVQEFFNLP 110

Query: 107 IEEKKRCRREVDGMEGYGNDLILSNQQILDWTDRLYLIVNPQDQRKLNFWPLNPQSFRED 166
           ++EK++  +     EG+G   I+S  Q LDW D   L   P   RK + +   P  FRE 
Sbjct: 111 MKEKQKLWQRSGEFEGFGQVNIVSENQKLDWGDMFILTTEPIRSRKSHLFSKLPPPFRET 170

Query: 167 LHEFTVKLMELIELVLIAMARSLNVDPNSFTEQVGNRPTLMTRFNFYPPCSTPHLVLGLK 226
           L  ++ ++  + +++   MA  L +      E + +      + N+YPPC  P  V+GL 
Sbjct: 171 LETYSSEVKSIAKILFAKMASVLEIKHEEM-EDLFDDVWQSIKINYYPPCPQPDQVMGLT 230

Query: 227 EHSDGSAITIILLDKQVEGFELRKDDQWYRVPVPTIADSLLINIGEQAEIMSNGIFKSPV 286
           +HSD + +TI+L   QVEG +++KD +W  V V  + D+L++N+GE  EI++NG ++S  
Sbjct: 231 QHSDAAGLTILLQVNQVEGLQIKKDGKW--VVVKPLRDALVVNVGEILEIITNGRYRSIE 290

Query: 287 HRAVTNSERQRISVACFCCPEKEIEIKPIEGLIDEKRPRMFRSV--KDYLQTYFLNYQTG 346
           HRAV NSE++R+SVA F  P KE  I+P + L+D ++  +F+S+  ++Y   +F     G
Sbjct: 291 HRAVVNSEKERLSVAMFHSPGKETIIRPAKSLVDRQKQCLFKSMSTQEYFDAFFTQKLNG 350

Query: 347 ERSVDGLRI 354
           +  +D +RI
Sbjct: 351 KSHLDLMRI 356

[Close]

BLAST of Lsi06G010220 vs. NCBI nr
Match: gi|659131148|ref|XP_008465534.1| (PREDICTED: protein SRG1-like isoform X2 [Cucumis melo])

HSP 1 Score: 606.3 bits (1562), Expect = 3.4e-170
Identity = 293/355 (82.54%), Postives = 320/355 (90.14%), Query Frame = 1


Query: 1   MAENSDL--TQTLQEKLLINGGDTPESYIYKGGYCGGDSNNNINPLPLAEIPVVDCSRLL 60
           MA+NS +  TQTLQ++LLINGG TPESYIYKGGY GGDSNNN  PLPLA+IPV+D S+L 
Sbjct: 1   MAQNSQIPQTQTLQQQLLINGGHTPESYIYKGGYHGGDSNNN-TPLPLAQIPVLDLSQLS 60

Query: 61  STPAGEAPLNELRLALSTWGCFQAINHNISSSFLEKMRKLSEQFFSLPIEEKKRCRREVD 120
           S   GEAPLN LRLALSTWGCFQA NH ISSSFLEKMRK+SEQFFSLPIEEK R  REVD
Sbjct: 61  SASVGEAPLNHLRLALSTWGCFQATNHGISSSFLEKMRKISEQFFSLPIEEKMRYGREVD 120

Query: 121 GMEGYGNDLILSNQQILDWTDRLYLIVNPQDQRKLNFWPLNPQSFREDLHEFTVKLMELI 180
           GMEGYGNDLI SNQQILDW+DRLYL+ NP+D+R+L FWPLNP SFREDLHE+TVKLM++I
Sbjct: 121 GMEGYGNDLIFSNQQILDWSDRLYLVTNPKDERRLEFWPLNPPSFREDLHEYTVKLMKII 180

Query: 181 ELVLIAMARSLNVDPNSFTEQVGNRPTLMTRFNFYPPCSTPHLVLGLKEHSDGSAITIIL 240
           + VLIAMARSLNV+PNSFT+QVG RPTL  RFNFYPPCSTPHLVLGLKEHSDG+AIT++L
Sbjct: 181 DTVLIAMARSLNVEPNSFTDQVGKRPTLFKRFNFYPPCSTPHLVLGLKEHSDGTAITVLL 240

Query: 241 LDKQVEGFELRKDDQWYRVPVPTIADSLLINIGEQAEIMSNGIFKSPVHRAVTNSERQRI 300
           LDKQVEG ELRKDDQWYRVPVP +ADSLLI IGEQAEIMSNGIFKS +HRAVTNSERQRI
Sbjct: 241 LDKQVEGLELRKDDQWYRVPVPAVADSLLIIIGEQAEIMSNGIFKSLIHRAVTNSERQRI 300

Query: 301 SVACFCCPEKEIEIKPIEGLIDEKRPRMFRSVKDYLQTYFLNYQTGERSVDGLRI 354
           SV  FCCPEK+IEIKPIEGLIDEKRPR+FRS K+YLQTYF NYQ GERSVDGLRI
Sbjct: 301 SVVSFCCPEKDIEIKPIEGLIDEKRPRLFRSTKNYLQTYFQNYQKGERSVDGLRI 354

[Close]

BLAST of Lsi06G010220 vs. NCBI nr
Match: gi|778659909|ref|XP_011655280.1| (PREDICTED: protein SRG1-like [Cucumis sativus])

HSP 1 Score: 601.7 bits (1550), Expect = 8.4e-169
Identity = 289/355 (81.41%), Postives = 320/355 (90.14%), Query Frame = 1


Query: 1   MAENSD--LTQTLQEKLLINGGDTPESYIYKGGYCGGDSNNNINPLPLAEIPVVDCSRLL 60
           MA+NS    TQTLQ++LLINGG TPESYIYKGGY GG SNNN  PLPLAEIPVVD S+L 
Sbjct: 1   MAQNSQNTQTQTLQQQLLINGGHTPESYIYKGGYHGGGSNNN-TPLPLAEIPVVDLSQLS 60

Query: 61  STPAGEAPLNELRLALSTWGCFQAINHNISSSFLEKMRKLSEQFFSLPIEEKKRCRREVD 120
           S  AGE PLN+LRLALSTWGCFQA NH+ISSSFLEK+RK+SEQFFSLPIEEK R  REVD
Sbjct: 61  SPSAGEGPLNDLRLALSTWGCFQATNHSISSSFLEKLRKISEQFFSLPIEEKMRYGREVD 120

Query: 121 GMEGYGNDLILSNQQILDWTDRLYLIVNPQDQRKLNFWPLNPQSFREDLHEFTVKLMELI 180
           GMEGYGNDL  SNQQ LDW+DRLY + +P+D+R+L+ WPLNP SFREDLHE+TVK+ME+I
Sbjct: 121 GMEGYGNDLTFSNQQTLDWSDRLYFVTSPEDERRLDLWPLNPPSFREDLHEYTVKIMEII 180

Query: 181 ELVLIAMARSLNVDPNSFTEQVGNRPTLMTRFNFYPPCSTPHLVLGLKEHSDGSAITIIL 240
           E VLIAMARSLNV+PNSFT+QVG RPTL TRFNFYPPCSTPHLVLGLKEHSDGSAITI+L
Sbjct: 181 ETVLIAMARSLNVEPNSFTDQVGERPTLFTRFNFYPPCSTPHLVLGLKEHSDGSAITILL 240

Query: 241 LDKQVEGFELRKDDQWYRVPVPTIADSLLINIGEQAEIMSNGIFKSPVHRAVTNSERQRI 300
           LDKQVEG +LRKDDQWYRVPVP IADSLL+ IGEQAE+MSNGIFKS VHRAVTNSERQRI
Sbjct: 241 LDKQVEGLQLRKDDQWYRVPVPAIADSLLLVIGEQAEVMSNGIFKSSVHRAVTNSERQRI 300

Query: 301 SVACFCCPEKEIEIKPIEGLIDEKRPRMFRSVKDYLQTYFLNYQTGERSVDGLRI 354
           SV CFCCPEK+IEIKP+EGLIDEKRPR+FRSVK+YL+TYF NYQ G+R+VDGLRI
Sbjct: 301 SVVCFCCPEKDIEIKPVEGLIDEKRPRLFRSVKNYLETYFQNYQEGQRTVDGLRI 354

[Close]

BLAST of Lsi06G010220 vs. NCBI nr
Match: gi|659131146|ref|XP_008465533.1| (PREDICTED: protein SRG1-like isoform X1 [Cucumis melo])

HSP 1 Score: 601.7 bits (1550), Expect = 8.4e-169
Identity = 293/356 (82.30%), Postives = 320/356 (89.89%), Query Frame = 1


Query: 1   MAENSDL--TQTLQEKLLINGGDTPESYIYKGGYCGGDSNNNINPLPLAEIPVVDCSRLL 60
           MA+NS +  TQTLQ++LLINGG TPESYIYKGGY GGDSNNN  PLPLA+IPV+D S+L 
Sbjct: 1   MAQNSQIPQTQTLQQQLLINGGHTPESYIYKGGYHGGDSNNN-TPLPLAQIPVLDLSQLS 60

Query: 61  STPAGEAPLNELRLALSTWGCFQAINHNISSSFLEKMRKLSEQFFSLPIEEKKRCRREVD 120
           S   GEAPLN LRLALSTWGCFQA NH ISSSFLEKMRK+SEQFFSLPIEEK R  REVD
Sbjct: 61  SASVGEAPLNHLRLALSTWGCFQATNHGISSSFLEKMRKISEQFFSLPIEEKMRYGREVD 120

Query: 121 GMEGYGNDLILSNQQILDWTDRLYLIVNPQDQRKLNFWPLNPQSFR-EDLHEFTVKLMEL 180
           GMEGYGNDLI SNQQILDW+DRLYL+ NP+D+R+L FWPLNP SFR EDLHE+TVKLM++
Sbjct: 121 GMEGYGNDLIFSNQQILDWSDRLYLVTNPKDERRLEFWPLNPPSFRSEDLHEYTVKLMKI 180

Query: 181 IELVLIAMARSLNVDPNSFTEQVGNRPTLMTRFNFYPPCSTPHLVLGLKEHSDGSAITII 240
           I+ VLIAMARSLNV+PNSFT+QVG RPTL  RFNFYPPCSTPHLVLGLKEHSDG+AIT++
Sbjct: 181 IDTVLIAMARSLNVEPNSFTDQVGKRPTLFKRFNFYPPCSTPHLVLGLKEHSDGTAITVL 240

Query: 241 LLDKQVEGFELRKDDQWYRVPVPTIADSLLINIGEQAEIMSNGIFKSPVHRAVTNSERQR 300
           LLDKQVEG ELRKDDQWYRVPVP +ADSLLI IGEQAEIMSNGIFKS +HRAVTNSERQR
Sbjct: 241 LLDKQVEGLELRKDDQWYRVPVPAVADSLLIIIGEQAEIMSNGIFKSLIHRAVTNSERQR 300

Query: 301 ISVACFCCPEKEIEIKPIEGLIDEKRPRMFRSVKDYLQTYFLNYQTGERSVDGLRI 354
           ISV  FCCPEK+IEIKPIEGLIDEKRPR+FRS K+YLQTYF NYQ GERSVDGLRI
Sbjct: 301 ISVVSFCCPEKDIEIKPIEGLIDEKRPRLFRSTKNYLQTYFQNYQKGERSVDGLRI 355

[Close]

BLAST of Lsi06G010220 vs. NCBI nr
Match: gi|778660153|ref|XP_011655698.1| (PREDICTED: protein SRG1-like [Cucumis sativus])

HSP 1 Score: 595.5 bits (1534), Expect = 6.0e-167
Identity = 287/355 (80.85%), Postives = 317/355 (89.30%), Query Frame = 1


Query: 1   MAENSD--LTQTLQEKLLINGGDTPESYIYKGGYCGGDSNNNINPLPLAEIPVVDCSRLL 60
           MA+NS    TQTLQ++LLINGG TPESYIYKGGY GG SNNN  PLPLAEIPVVD S+L 
Sbjct: 1   MAQNSQNTQTQTLQQQLLINGGHTPESYIYKGGYHGGGSNNN-TPLPLAEIPVVDLSQLS 60

Query: 61  STPAGEAPLNELRLALSTWGCFQAINHNISSSFLEKMRKLSEQFFSLPIEEKKRCRREVD 120
           S  AGE PLN+LRLALSTWGCFQAINH+ISSSFLEKMRK+SEQFFSLPIEEK R  REVD
Sbjct: 61  SPSAGEGPLNDLRLALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVD 120

Query: 121 GMEGYGNDLILSNQQILDWTDRLYLIVNPQDQRKLNFWPLNPQSFREDLHEFTVKLMELI 180
           GMEGYGNDL  SNQQ LDW+DRLY + NP+D+R+L  WPLNP SFREDL E+TVK+ME+I
Sbjct: 121 GMEGYGNDLTFSNQQTLDWSDRLYFVTNPEDERRLELWPLNPPSFREDLDEYTVKIMEII 180

Query: 181 ELVLIAMARSLNVDPNSFTEQVGNRPTLMTRFNFYPPCSTPHLVLGLKEHSDGSAITIIL 240
           E VLIAMA SL+V+PNSFT+QVG RPTL+TRFNFYPPCSTPHLVLGLKEHSDGSAITI+L
Sbjct: 181 ETVLIAMASSLDVEPNSFTDQVGKRPTLLTRFNFYPPCSTPHLVLGLKEHSDGSAITILL 240

Query: 241 LDKQVEGFELRKDDQWYRVPVPTIADSLLINIGEQAEIMSNGIFKSPVHRAVTNSERQRI 300
           LDKQVEG +LRKDDQWYRVPVP IADSLL+ IGEQAE+MSNGIFKS +HRAVTNSERQRI
Sbjct: 241 LDKQVEGLQLRKDDQWYRVPVPAIADSLLLVIGEQAEVMSNGIFKSSIHRAVTNSERQRI 300

Query: 301 SVACFCCPEKEIEIKPIEGLIDEKRPRMFRSVKDYLQTYFLNYQTGERSVDGLRI 354
           S+ CFCCPEK+IEIKPIEGLIDEKRPR+F+SVK+YL+TYF NYQ G R VDGLRI
Sbjct: 301 SLVCFCCPEKDIEIKPIEGLIDEKRPRLFKSVKNYLETYFQNYQKGRRPVDGLRI 354

[Close]

BLAST of Lsi06G010220 vs. NCBI nr
Match: gi|700210001|gb|KGN65097.1| (hypothetical protein Csa_1G212830 [Cucumis sativus])

HSP 1 Score: 523.1 bits (1346), Expect = 3.8e-145
Identity = 246/300 (82.00%), Postives = 275/300 (91.67%), Query Frame = 1


Query: 55  SRLLSTP-AGEAPLNELRLALSTWGCFQAINHNISSSFLEKMRKLSEQFFSLPIEEKKRC 114
           +R L +P AGE PLN+LRLALSTWGCFQA NH+ISSSFLEK+RK+SEQFFSLPIEEK R 
Sbjct: 278 NRYLKSPSAGEGPLNDLRLALSTWGCFQATNHSISSSFLEKLRKISEQFFSLPIEEKMRY 337

Query: 115 RREVDGMEGYGNDLILSNQQILDWTDRLYLIVNPQDQRKLNFWPLNPQSFREDLHEFTVK 174
            REVDGMEGYGNDL  SNQQ LDW+DRLY + +P+D+R+L+ WPLNP SFREDLHE+TVK
Sbjct: 338 GREVDGMEGYGNDLTFSNQQTLDWSDRLYFVTSPEDERRLDLWPLNPPSFREDLHEYTVK 397

Query: 175 LMELIELVLIAMARSLNVDPNSFTEQVGNRPTLMTRFNFYPPCSTPHLVLGLKEHSDGSA 234
           +ME+IE VLIAMARSLNV+PNSFT+QVG RPTL TRFNFYPPCSTPHLVLGLKEHSDGSA
Sbjct: 398 IMEIIETVLIAMARSLNVEPNSFTDQVGERPTLFTRFNFYPPCSTPHLVLGLKEHSDGSA 457

Query: 235 ITIILLDKQVEGFELRKDDQWYRVPVPTIADSLLINIGEQAEIMSNGIFKSPVHRAVTNS 294
           ITI+LLDKQVEG +LRKDDQWYRVPVP IADSLL+ IGEQAE+MSNGIFKS VHRAVTNS
Sbjct: 458 ITILLLDKQVEGLQLRKDDQWYRVPVPAIADSLLLVIGEQAEVMSNGIFKSSVHRAVTNS 517

Query: 295 ERQRISVACFCCPEKEIEIKPIEGLIDEKRPRMFRSVKDYLQTYFLNYQTGERSVDGLRI 354
           ERQRISV CFCCPEK+IEIKP+EGLIDEKRPR+FRSVK+YL+TYF NYQ G+R+VDGLRI
Sbjct: 518 ERQRISVVCFCCPEKDIEIKPVEGLIDEKRPRLFRSVKNYLETYFQNYQEGQRTVDGLRI 577

The following BLAST results are available for this feature:

BLAST of Lsi06G010220 vs. Swiss-Prot


Match Name	E-value	Identity	Description
SRG1_ARATH	2.5e-56	34.30	Protein SRG1 OS=Arabidopsis thaliana GN=SRG1 PE=2 SV=1	[more]
NCS1_COPJA	1.4e-51	37.59	S-norcoclaurine synthase 1 OS=Coptis japonica GN=NCS1 PE=1 SV=1	[more]
DIOX3_PAPSO	2.7e-50	33.24	Codeine O-demethylase OS=Papaver somniferum GN=CODM PE=1 SV=1	[more]
DIOX2_PAPSO	1.8e-46	31.73	Probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Papaver somniferum GN=DI...	[more]
DIOX1_PAPSO	9.0e-46	33.56	Thebaine 6-O-demethylase OS=Papaver somniferum GN=T6ODM PE=1 SV=1	[more]

BLAST of Lsi06G010220 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LW96_CUCSA	2.7e-145	82.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G212830 PE=3 SV=1	[more]
U7E2X6_POPTR	6.4e-107	51.84	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0025s00430g PE=3 SV=1	[more]
B9N703_POPTR	1.5e-103	50.14	Oxidoreductase family protein OS=Populus trichocarpa GN=POPTR_0006s06130g PE=3 S...	[more]
A0A059CLG0_EUCGR	5.6e-103	51.15	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_C00494 PE=3 SV=1	[more]
U7E2D8_POPTR	4.7e-102	51.32	Oxidoreductase family protein OS=Populus trichocarpa GN=POPTR_0025s00440g PE=3 S...	[more]

BLAST of Lsi06G010220 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G20400.1	7.5e-99	48.86	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT1G49390.1	1.4e-92	46.82	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT5G54000.1	1.3e-90	46.24	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT5G20550.1	4.1e-89	46.49	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT1G78550.1	4.4e-59	37.22	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]

BLAST of Lsi06G010220 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659131148\|ref\|XP_008465534.1\|	3.4e-170	82.54	PREDICTED: protein SRG1-like isoform X2 [Cucumis melo]	[more]
gi\|778659909\|ref\|XP_011655280.1\|	8.4e-169	81.41	PREDICTED: protein SRG1-like [Cucumis sativus]	[more]
gi\|659131146\|ref\|XP_008465533.1\|	8.4e-169	82.30	PREDICTED: protein SRG1-like isoform X1 [Cucumis melo]	[more]
gi\|778660153\|ref\|XP_011655698.1\|	6.0e-167	80.85	PREDICTED: protein SRG1-like [Cucumis sativus]	[more]
gi\|700210001\|gb\|KGN65097.1\|	3.8e-145	82.00	hypothetical protein Csa_1G212830 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR027443	IPNS-like
IPR026992	DIOX_N
IPR005123	Oxoglu/Fe-dep_dioxygenase

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0016706	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi06G010220.1	Lsi06G010220.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PFAM	PF03171	2OG-FeII_Oxy	coord: 207..306 score: 5.4
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROFILE	PS51471	FE2OG_OXY	coord: 204..306 score: 11
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 49..157 score: 6.3
IPR027443	Isopenicillin N synthase-like	GENE3D	G3DSA:2.60.120.330		coord: 40..347 score: 4.8
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 1..347 score: 2.4E
None	No IPR available	PANTHER	PTHR10209:SF107	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE-LIKE PROTEIN-RELATED	coord: 1..347 score: 2.4E
None	No IPR available	unknown	SSF51197	Clavaminate synthase-like	coord: 14..341 score: 2.2

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi06G010220	Csa1G212830	Cucumber (Chinese Long) v2	culsiB074
Lsi06G010220	CSPI01G16450	Wild cucumber (PI 183967)	cpilsiB074
Lsi06G010220	CmaCh11G005090	Cucurbita maxima (Rimu)	cmalsiB147
Lsi06G010220	CmaCh10G005090	Cucurbita maxima (Rimu)	cmalsiB074
Lsi06G010220	CmoCh11G005060	Cucurbita moschata (Rifu)	cmolsiB131
Lsi06G010220	CmoCh10G005460	Cucurbita moschata (Rifu)	cmolsiB060
Lsi06G010220	Cla012183	Watermelon (97103) v1	lsiwmB415
Lsi06G010220	ClCG06G011790	Watermelon (Charleston Gray)	lsiwcgB373
Lsi06G010220	Cla97C06G120950	Watermelon (97103) v2	lsiwmbB375
Lsi06G010220	Bhi02G000754	Wax gourd	lsiwgoB518
Lsi06G010220	Cp4.1LG04g09110	Cucurbita pepo (Zucchini)	cpelsiB557
Lsi06G010220	Cp4.1LG18g05360	Cucurbita pepo (Zucchini)	cpelsiB300
Lsi06G010220	CsGy1G017260	Cucumber (Gy14) v2	cgyblsiB063
Lsi06G010220	Carg09953	Silver-seed gourd	carlsiB115
Lsi06G010220	CsaV3_1G028060	Cucumber (Chinese Long) v3	cuclsiB073

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi06G010220	Melon (DHL92) v3.6.1	lsimedB425
Lsi06G010220	Silver-seed gourd	carlsiB578
Lsi06G010220	Bottle gourd (USVL1VR-Ls)	lsilsiB057
Lsi06G010220	Cucurbita maxima (Rimu)	cmalsiB031
Lsi06G010220	Cucurbita moschata (Rifu)	cmolsiB019
Lsi06G010220	Cucurbita pepo (Zucchini)	cpelsiB565
Lsi06G010220	Cucurbita pepo (Zucchini)	cpelsiB643
Lsi06G010220	Melon (DHL92) v3.5.1	lsimeB403
Lsi06G010220	Watermelon (Charleston Gray)	lsiwcgB354
Lsi06G010220	Watermelon (Charleston Gray)	lsiwcgB371
Lsi06G010220	Watermelon (97103) v1	lsiwmB405