Lsi06G007850 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi06G007850
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionRNA-directed DNA polymerase (Reverse transcriptase), Ribonuclease H
Locationchr06 : 15969647 .. 15970216 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATCCTATAAAGTATATTTTCGAGAAAACATCATTGTCTGGAAGAATAGCCAAGTGGCAAGTATTGCTATCTGAGTATGATATTGTTTATGTCACTAGAAAAGCCATAAAAGGGTGCGCAATCGCCGATTGCCTAGCTGAGTTACCAGTTGAAGACTATGAACCGATGAATTTTGAGTTTCTAGATGAAGATATTATGACGATAAAAGCACTCCAGGATATAGAAACATGGACAATGTTGTTTGATGGGGCCACGAACGAGGTAGGACATGGTGTAGGAGCTATTTTGATATCTCCAAAAAGGAAGTTATATAGCCTGACGGCAAAGTTGTACTTCGATTGCACGAATAACATGGCCGAATATGAGGCATGTAGTATGGGAGTTCAAATGGCCTTCAATATGAAGATTAAAAAGTTGAAAGTTTATGGAGATTTCCTTTTAGTAGTGCATCAACTATGTGGGGAATGGGAGACAAGAGACCCTAAATTGATTCCCTATCATCAATACATCCGAGAGTTGGCTCAAAATTTCGAATCGATCACATTTGAGCATATCATGCGAAAATAA

mRNA sequence

ATGGATCCTATAAAGTATATTTTCGAGAAAACATCATTGTCTGGAAGAATAGCCAAGTGGCAAGTATTGCTATCTGAGTATGATATTGTTTATGTCACTAGAAAAGCCATAAAAGGGTGCGCAATCGCCGATTGCCTAGCTGAGTTACCAGTTGAAGACTATGAACCGATGAATTTTGAGTTTCTAGATGAAGATATTATGACGATAAAAGCACTCCAGGATATAGAAACATGGACAATGTTGTTTGATGGGGCCACGAACGAGGTAGGACATGGTGTAGGAGCTATTTTGATATCTCCAAAAAGGAAGTTATATAGCCTGACGGCAAAGTTGTACTTCGATTGCACGAATAACATGGCCGAATATGAGGCATGTAGTATGGGAGTTCAAATGGCCTTCAATATGAAGATTAAAAAGTTGAAAGTTTATGGAGATTTCCTTTTAGTAGTGCATCAACTATGTGGGGAATGGGAGACAAGAGACCCTAAATTGATTCCCTATCATCAATACATCCGAGAGTTGGCTCAAAATTTCGAATCGATCACATTTGAGCATATCATGCGAAAATAA

Coding sequence (CDS)

ATGGATCCTATAAAGTATATTTTCGAGAAAACATCATTGTCTGGAAGAATAGCCAAGTGGCAAGTATTGCTATCTGAGTATGATATTGTTTATGTCACTAGAAAAGCCATAAAAGGGTGCGCAATCGCCGATTGCCTAGCTGAGTTACCAGTTGAAGACTATGAACCGATGAATTTTGAGTTTCTAGATGAAGATATTATGACGATAAAAGCACTCCAGGATATAGAAACATGGACAATGTTGTTTGATGGGGCCACGAACGAGGTAGGACATGGTGTAGGAGCTATTTTGATATCTCCAAAAAGGAAGTTATATAGCCTGACGGCAAAGTTGTACTTCGATTGCACGAATAACATGGCCGAATATGAGGCATGTAGTATGGGAGTTCAAATGGCCTTCAATATGAAGATTAAAAAGTTGAAAGTTTATGGAGATTTCCTTTTAGTAGTGCATCAACTATGTGGGGAATGGGAGACAAGAGACCCTAAATTGATTCCCTATCATCAATACATCCGAGAGTTGGCTCAAAATTTCGAATCGATCACATTTGAGCATATCATGCGAAAATAA

Protein sequence

MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFEFLDEDIMTIKALQDIETWTMLFDGATNEVGHGVGAILISPKRKLYSLTAKLYFDCTNNMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQNFESITFEHIMRK
Homology
[Close]
BLAST of Lsi06G007850 vs. Swiss-Prot
Match: RNH_HALSA (Ribonuclease HI OS=Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) GN=rnhA PE=1 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 2.6e-07
Identity = 35/109 (32.11%), Postives = 51/109 (46.79%), Query Frame = 1 

Query: 82  FDGAT--NEVGHGVGAILISPKRKLYSLTAKLYFDCTNNMAEYEACSMGVQMAFNMKIKK 141
           FDGA+  N     VG +L+S    + +         TNN AEY+A    ++ A +     
Sbjct: 74  FDGASRGNPGPAAVGWVLVSGDGGIVAEGGDTIGRATNNQAEYDALIAALEAAADFGFDD 133

Query: 142 LKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQNFESITFEHIMR 189
           +++ GD  LV  QL G W+T DP L       REL   F+  +  H+ R
Sbjct: 134 IELRGDSQLVEKQLTGAWDTNDPDLRRKRVRARELLTGFDDWSITHVPR 182

[Close]
BLAST of Lsi06G007850 vs. TrEMBL
Match: A0A061DRM3_THECC (RNA-directed DNA polymerase (Reverse transcriptase), Ribonuclease H OS=Theobroma cacao GN=TCM_004328 PE=4 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 6.8e-63
Identity = 118/193 (61.14%), Postives = 146/193 (75.65%), Query Frame = 1 

Query: 1   MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFE 60
           +DPIKYIFEK SLSGR+A+WQVLLSEYDIVYV++KAIKG AIAD LAE   EDYEPM FE
Sbjct: 19  LDPIKYIFEKPSLSGRVARWQVLLSEYDIVYVSQKAIKGSAIADFLAERVEEDYEPMEFE 78

Query: 61  FLDEDIMTIKALQ----DIETWTMLFDGATNEVGHGVGAILISPKRKLYSLTAKLYFDCT 120
           F DED+M+I        + E W M FDGA+N +GHG+G +L+SP+   Y + AKL F CT
Sbjct: 79  FPDEDLMSICQTSGEESEKENWKMFFDGASNALGHGIGVVLVSPEGDHYPVIAKLNFYCT 138

Query: 121 NNMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQ 180
           NN+AEYEAC MG+Q A   KI  L+VYGD  LV++QL GEWETRD KL+ YH+Y+ +L +
Sbjct: 139 NNVAEYEACVMGIQAAIERKIHILEVYGDSALVIYQLRGEWETRDSKLVRYHKYVSKLIE 198

Query: 181 NFESITFEHIMRK 190
           NF+ I F H+ R+
Sbjct: 199 NFDEICFNHLPRE 211

[Close]
BLAST of Lsi06G007850 vs. TrEMBL
Match: A0A061EFI0_THECC (RNA-directed DNA polymerase (Reverse transcriptase), Ribonuclease H OS=Theobroma cacao GN=TCM_010987 PE=4 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 6.8e-63
Identity = 118/193 (61.14%), Postives = 146/193 (75.65%), Query Frame = 1 

Query: 1   MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFE 60
           +DPIKYIFEK SLSGR+A+WQVLLSEYDIVYV++KAIKG AIAD LAE   EDYEPM FE
Sbjct: 364 LDPIKYIFEKPSLSGRVARWQVLLSEYDIVYVSQKAIKGSAIADFLAERVEEDYEPMEFE 423

Query: 61  FLDEDIMTIKALQ----DIETWTMLFDGATNEVGHGVGAILISPKRKLYSLTAKLYFDCT 120
           F DED+M+I        + E W M FDGA+N +GHG+G +L+SP+   Y + AKL F CT
Sbjct: 424 FPDEDLMSICQTSGEESEKENWKMFFDGASNALGHGIGVVLVSPEGDHYPVIAKLNFYCT 483

Query: 121 NNMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQ 180
           NN+AEYEAC MG+Q A   KI  L+VYGD  LV++QL GEWETRD KL+ YH+Y+ +L +
Sbjct: 484 NNVAEYEACVMGIQAAIERKIHILEVYGDSALVIYQLRGEWETRDSKLVRYHKYVSKLIE 543

Query: 181 NFESITFEHIMRK 190
           NF+ I F H+ R+
Sbjct: 544 NFDEICFNHLPRE 556

[Close]
BLAST of Lsi06G007850 vs. TrEMBL
Match: A0A061FRJ8_THECC (RNA-directed DNA polymerase, putative OS=Theobroma cacao GN=TCM_045360 PE=4 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 3.4e-62
Identity = 118/193 (61.14%), Postives = 146/193 (75.65%), Query Frame = 1 

Query: 1    MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFE 60
            +DPIKYIFEK SLSGR+A+WQVLLSEYDI+YV++KAIKG AIAD LAE   EDYEPM FE
Sbjct: 1238 LDPIKYIFEKPSLSGRVARWQVLLSEYDIIYVSQKAIKGSAIADFLAERVEEDYEPMEFE 1297

Query: 61   FLDEDIMTIKALQDIET----WTMLFDGATNEVGHGVGAILISPKRKLYSLTAKLYFDCT 120
            F DED+M+I      E+    W M FDGA+N +GHGVG +L+SP+   Y + AKL F CT
Sbjct: 1298 FPDEDLMSICQTNGEESKNENWKMFFDGASNALGHGVGVVLVSPEGDHYPVIAKLDFYCT 1357

Query: 121  NNMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQ 180
            NN+AEYEAC MG+Q A   KI  L+VYGD  LV++QL GEWETRD KL+ YH+Y+ +L +
Sbjct: 1358 NNVAEYEACVMGLQAAIERKIHILEVYGDSALVIYQLRGEWETRDSKLVRYHKYVSKLIE 1417

Query: 181  NFESITFEHIMRK 190
            NF+ I F H+ R+
Sbjct: 1418 NFDKICFTHLPRE 1430

[Close]
BLAST of Lsi06G007850 vs. TrEMBL
Match: A0A061GEQ1_THECC (RNA-directed DNA polymerase (Reverse transcriptase), Ribonuclease H-like protein OS=Theobroma cacao GN=TCM_029731 PE=4 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 2.7e-59
Identity = 109/194 (56.19%), Postives = 147/194 (75.77%), Query Frame = 1 

Query: 1    MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFE 60
            +DPIKYIFEK  LSGRIA+WQVLLSEYD+VYV++K+IKG AIAD LA+   EDYE ++F+
Sbjct: 1242 LDPIKYIFEKPCLSGRIARWQVLLSEYDLVYVSQKSIKGSAIADFLADRANEDYESVSFD 1301

Query: 61   FLDEDIMTIKALQ-----DIETWTMLFDGATNEVGHGVGAILISPKRKLYSLTAKLYFDC 120
            F DED+M +  ++     ++  W + FDGA+N +GHG+GA+LISP  K Y  TA+L F+C
Sbjct: 1302 FPDEDLMAVLHVEKVGPNELNPWKVFFDGASNALGHGIGAVLISPNGKYYPATARLNFNC 1361

Query: 121  TNNMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELA 180
            TNNMAEYEA  +G+Q A +MK   + VYGD  LV+ Q+ GEWETRDPKL+PY + + EL+
Sbjct: 1362 TNNMAEYEALVLGLQAAIDMKADAIDVYGDSALVICQMKGEWETRDPKLVPYKKLVTELS 1421

Query: 181  QNFESITFEHIMRK 190
            + F+ I+F H+ R+
Sbjct: 1422 KQFKEISFNHLPRE 1435

[Close]
BLAST of Lsi06G007850 vs. TrEMBL
Match: A0A061FVT2_THECC (RNA-directed DNA polymerase (Reverse transcriptase), Ribonuclease H OS=Theobroma cacao GN=TCM_012304 PE=4 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 1.0e-58
Identity = 109/194 (56.19%), Postives = 144/194 (74.23%), Query Frame = 1 

Query: 1   MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFE 60
           +DPIKYIFEK  LSGRIA+WQVLLSEYDIVYV++K+IKG AI D LA+   EDYE ++F+
Sbjct: 405 LDPIKYIFEKPCLSGRIARWQVLLSEYDIVYVSQKSIKGSAIVDFLADRANEDYESVSFD 464

Query: 61  FLDEDIMTIKALQ-----DIETWTMLFDGATNEVGHGVGAILISPKRKLYSLTAKLYFDC 120
           F DED+M +  ++     ++  W + FDGA+N +GHG+GA+LISP  K Y  T +L F+C
Sbjct: 465 FPDEDLMAVLHVEKVGPNELNPWKVYFDGASNALGHGIGAVLISPNGKYYPATTRLNFNC 524

Query: 121 TNNMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELA 180
           TNNMAEYEA  MG+Q A  MK   + VYGD  LV+ Q+ GEWETRDPKL+PY + + EL+
Sbjct: 525 TNNMAEYEALVMGLQAAIEMKADAIDVYGDSALVICQMKGEWETRDPKLVPYKKLVTELS 584

Query: 181 QNFESITFEHIMRK 190
           + F+ I+F H+ R+
Sbjct: 585 KQFKEISFNHLPRE 598

[Close]
BLAST of Lsi06G007850 vs. TAIR10
Match: AT3G01410.1 (AT3G01410.1 Polynucleotidyl transferase, ribonuclease H-like superfamily protein)

HSP 1 Score: 70.9 bits (172), Expect = 9.8e-13
Identity = 38/130 (29.23%), Postives = 69/130 (53.08%), Query Frame = 1 

Query: 62  LDEDIMTIKALQDIETWTMLFDGAT--NEVGHGVGAILISPKRKLYSLTAKLYFDCTNNM 121
           +   ++T   ++  ++ T+ FDGA+  N    G GA+L +    +     +   + TNN+
Sbjct: 139 IPSSLLTRTPIRQNDSCTIEFDGASKGNPGKAGAGAVLRASDNSVLFYLREGVGNATNNV 198

Query: 122 AEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQNFE 181
           AEY A  +G++ A +   K + V GD +LV  Q+ G W+T  PK+    +  +EL  +F+
Sbjct: 199 AEYRALLLGLRSALDKGFKNVHVLGDSMLVCMQVQGAWKTNHPKMAELCKQAKELMNSFK 258

Query: 182 SITFEHIMRK 190
           +   +HI R+
Sbjct: 259 TFDIKHIARE 268

[Close]
BLAST of Lsi06G007850 vs. TAIR10
Match: AT1G24090.1 (AT1G24090.1 RNase H family protein)

HSP 1 Score: 57.0 bits (136), Expect = 1.5e-08
Identity = 36/115 (31.30%), Postives = 52/115 (45.22%), Query Frame = 1 

Query: 76  ETWTMLFDGAT--NEVGHGVGAILISPKRKLYSLTAKLYFDCTNNMAEYEACSMGVQMAF 135
           ET  + FDGA+  N    G  A+L +    L     +     TNN AEY A  +G++ A 
Sbjct: 215 ETCFIEFDGASKGNPGLSGAAAVLKTEDGSLICRVRQGLGIATNNAAEYHALILGLKYAI 274

Query: 136 NMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQNFESITFEHIMR 189
               K +KV GD  LV  Q+ G+W+     L   H+  + L     S    H++R
Sbjct: 275 EKGYKNIKVKGDSKLVCMQIKGQWKVNHEVLAKLHKEAKLLCNKCVSFEISHVLR 329

[Close]
BLAST of Lsi06G007850 vs. TAIR10
Match: AT5G51080.1 (AT5G51080.1 RNase H family protein)

HSP 1 Score: 52.8 bits (125), Expect = 2.8e-07
Identity = 34/115 (29.57%), Postives = 52/115 (45.22%), Query Frame = 1 

Query: 76  ETWTMLFDGAT--NEVGHGVGAILISPKRKLYSLTAKLYFDCTNNMAEYEACSMGVQMAF 135
           ET  + FDGA+  N    G  A+L +    L     +     TNN AEY    +G++ A 
Sbjct: 184 ETCIIEFDGASKGNPGLSGAAAVLKTEDGSLIFKMRQGLGIATNNAAEYHGLILGLKHAI 243

Query: 136 NMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQNFESITFEHIMR 189
                K+KV  D  LV  Q+ G+W+     L   H+  ++L+    S    H++R
Sbjct: 244 EKGYTKIKVKTDSKLVCMQMKGQWKVNHEVLSKLHKEAKQLSDKCLSFEISHVLR 298

[Close]
BLAST of Lsi06G007850 vs. NCBI nr
Match: gi|659092961|ref|XP_008447313.1| (PREDICTED: uncharacterized protein LOC103489784 [Cucumis melo])

HSP 1 Score: 272.7 bits (696), Expect = 4.8e-70
Identity = 129/186 (69.35%), Postives = 149/186 (80.11%), Query Frame = 1 

Query: 1   MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFE 60
           MDPIKYIFEK SLS +IAKW VLL E+DIVY+TRKAIKG  I DCL ELP+EDYEPM F+
Sbjct: 64  MDPIKYIFEKPSLSEKIAKWHVLLLEFDIVYITRKAIKGSVIVDCLVELPIEDYEPMKFD 123

Query: 61  FLDEDIMTIKALQDIETWTMLFDGATNEVGHGVGAILISPKRKLYSLTAKLYFDCTNNMA 120
           FLDEDIMTI      +TWTM+FD ATN+VGHG+G  L+SP  K Y  T KLYFDCTNNM 
Sbjct: 124 FLDEDIMTIVNTSS-DTWTMMFDRATNKVGHGMGVTLMSPDEKRYPFTTKLYFDCTNNMV 183

Query: 121 EYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQNFES 180
           EY+ACSMGV++A+ MKIK L+V GD LLVVH+L GE+ETRD K IPY+ YI  +AQ F+S
Sbjct: 184 EYKACSMGVRIAYEMKIKNLRVLGDSLLVVHKLNGEYETRDAKFIPYNDYICTIAQTFDS 243

Query: 181 ITFEHI 187
           I FEHI
Sbjct: 244 IMFEHI 248

[Close]
BLAST of Lsi06G007850 vs. NCBI nr
Match: gi|955329124|ref|XP_014633064.1| (PREDICTED: uncharacterized protein LOC106799391, partial [Glycine max])

HSP 1 Score: 264.2 bits (674), Expect = 1.7e-67
Identity = 123/192 (64.06%), Postives = 154/192 (80.21%), Query Frame = 1 

Query: 1   MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFE 60
           MDP+KYIFEK +L+GRIA+WQVLLSE+DIVYVT+KAIKG A+ADCLA+ P+ DY+PM+ E
Sbjct: 272 MDPVKYIFEKPALTGRIARWQVLLSEFDIVYVTQKAIKGSALADCLAQQPLNDYQPMHPE 331

Query: 61  FLDEDIMTI---KALQDIETWTMLFDGATNEVGHGVGAILISPKRKLYSLTAKLYFDCTN 120
           F DEDIM +   K  +D + WT+ FDGA+N +GHGVGA+LISP  +    TA+L FDCTN
Sbjct: 332 FPDEDIMALFEEKLDEDRDKWTVWFDGASNILGHGVGAVLISPDNQCVPFTARLGFDCTN 391

Query: 121 NMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQN 180
           NMAEYEAC++ VQ A +  +K LKVYGD  LV+HQL GEWETRDPKLIPY  YI+ELA+ 
Sbjct: 392 NMAEYEACALAVQAAIDSDVKLLKVYGDSALVIHQLRGEWETRDPKLIPYKAYIKELAKT 451

Query: 181 FESITFEHIMRK 190
           F+ I+F H+ R+
Sbjct: 452 FDEISFHHVPRE 463

[Close]
BLAST of Lsi06G007850 vs. NCBI nr
Match: gi|955326832|ref|XP_014632480.1| (PREDICTED: uncharacterized protein LOC106799045, partial [Glycine max])

HSP 1 Score: 260.8 bits (665), Expect = 1.9e-66
Identity = 121/192 (63.02%), Postives = 154/192 (80.21%), Query Frame = 1 

Query: 1   MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFE 60
           MDP+KYIFEK +L+GRIA+WQVLLSE+DIVYVT+KAIKG A+AD LA+ P+ DY+PM+ E
Sbjct: 677 MDPVKYIFEKPALTGRIARWQVLLSEFDIVYVTQKAIKGSALADYLAQQPLNDYQPMHPE 736

Query: 61  FLDEDIMTI---KALQDIETWTMLFDGATNEVGHGVGAILISPKRKLYSLTAKLYFDCTN 120
           F DEDIM +   K+ +D + WT+ FDGA+N +GHGVGA+L+SP  +    TA+L FDCTN
Sbjct: 737 FPDEDIMALFEEKSDEDRDKWTVWFDGASNILGHGVGAVLVSPDNQCVPFTARLGFDCTN 796

Query: 121 NMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQN 180
           NMAEYEAC++ VQ A +  +K LKVYGD  LV+HQL GEWETRDPKLIPY  YI+ELA+ 
Sbjct: 797 NMAEYEACALAVQAAIDSNVKLLKVYGDSALVIHQLRGEWETRDPKLIPYKAYIKELAKT 856

Query: 181 FESITFEHIMRK 190
           F+ I+F H+ R+
Sbjct: 857 FDEISFHHVPRE 868

[Close]
BLAST of Lsi06G007850 vs. NCBI nr
Match: gi|955333235|ref|XP_014633953.1| (PREDICTED: uncharacterized protein LOC106799574, partial [Glycine max])

HSP 1 Score: 260.8 bits (665), Expect = 1.9e-66
Identity = 123/192 (64.06%), Postives = 153/192 (79.69%), Query Frame = 1 

Query: 1    MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFE 60
            MDP+KYIFEK +L+GRIA+WQVLLSE+DIVYVT+KAIKG A+AD LA+ P+ DY+PM+ E
Sbjct: 866  MDPVKYIFEKPALTGRIARWQVLLSEFDIVYVTQKAIKGSALADYLAQQPLNDYQPMHPE 925

Query: 61   FLDEDIMTI---KALQDIETWTMLFDGATNEVGHGVGAILISPKRKLYSLTAKLYFDCTN 120
            F DEDIM +   K  +D + WT+ FDGA+N +GHGVGA+LISP  +    TA+L FDCTN
Sbjct: 926  FPDEDIMALFEEKLDEDRDKWTVWFDGASNILGHGVGAVLISPDNQCVPFTARLGFDCTN 985

Query: 121  NMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQN 180
            NMAEYEAC++ VQ A +  +K LKVYGD  LV+HQL GEWETRDPKLIPY  YI+ELA+ 
Sbjct: 986  NMAEYEACALAVQAAIDSDVKLLKVYGDSALVIHQLRGEWETRDPKLIPYKAYIKELAET 1045

Query: 181  FESITFEHIMRK 190
            F+ I+F HI R+
Sbjct: 1046 FDEISFHHIPRE 1057

[Close]
BLAST of Lsi06G007850 vs. NCBI nr
Match: gi|955353768|ref|XP_014620239.1| (PREDICTED: uncharacterized protein LOC106795329, partial [Glycine max])

HSP 1 Score: 260.8 bits (665), Expect = 1.9e-66
Identity = 122/192 (63.54%), Postives = 153/192 (79.69%), Query Frame = 1 

Query: 1    MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFE 60
            MDP+KYIFEK +L+GRIA+WQVLLSE+DIVYVT+KAIKG A+AD LA+ P+ DY+PM+ E
Sbjct: 866  MDPVKYIFEKPALTGRIARWQVLLSEFDIVYVTQKAIKGSALADYLAQQPLNDYQPMHPE 925

Query: 61   FLDEDIMTI---KALQDIETWTMLFDGATNEVGHGVGAILISPKRKLYSLTAKLYFDCTN 120
            F DEDIM +   K  +D + WT+ FDGA+N +GHGVGA+LISP  +    TA+L FDCTN
Sbjct: 926  FPDEDIMALFEEKLDEDRDKWTVWFDGASNVLGHGVGAVLISPDNQCVPFTARLGFDCTN 985

Query: 121  NMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQN 180
            NMAEYEAC++ VQ A +  +K LKVYGD  LV+HQL GEWETRDPKLIPY  YI+ELA+ 
Sbjct: 986  NMAEYEACALAVQAAIDSDVKLLKVYGDSALVIHQLRGEWETRDPKLIPYKAYIKELAET 1045

Query: 181  FESITFEHIMRK 190
            F+ I+F H+ R+
Sbjct: 1046 FDEISFHHVPRE 1057

The following BLAST results are available for this feature:
BLAST of Lsi06G007850 vs. Swiss-Prot
Match NameE-valueIdentityDescription
RNH_HALSA2.6e-0732.11Ribonuclease HI OS=Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC... [more]
BLAST of Lsi06G007850 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A061DRM3_THECC6.8e-6361.14RNA-directed DNA polymerase (Reverse transcriptase), Ribonuclease H OS=Theobroma... [more]
A0A061EFI0_THECC6.8e-6361.14RNA-directed DNA polymerase (Reverse transcriptase), Ribonuclease H OS=Theobroma... [more]
A0A061FRJ8_THECC3.4e-6261.14RNA-directed DNA polymerase, putative OS=Theobroma cacao GN=TCM_045360 PE=4 SV=1[more]
A0A061GEQ1_THECC2.7e-5956.19RNA-directed DNA polymerase (Reverse transcriptase), Ribonuclease H-like protein... [more]
A0A061FVT2_THECC1.0e-5856.19RNA-directed DNA polymerase (Reverse transcriptase), Ribonuclease H OS=Theobroma... [more]
BLAST of Lsi06G007850 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G01410.19.8e-1329.23 Polynucleotidyl transferase, ribonuclease H-like superfamily protein[more]
AT1G24090.11.5e-0831.30 RNase H family protein[more]
AT5G51080.12.8e-0729.57 RNase H family protein[more]
BLAST of Lsi06G007850 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659092961|ref|XP_008447313.1|4.8e-7069.35PREDICTED: uncharacterized protein LOC103489784 [Cucumis melo][more]
gi|955329124|ref|XP_014633064.1|1.7e-6764.06PREDICTED: uncharacterized protein LOC106799391, partial [Glycine max][more]
gi|955326832|ref|XP_014632480.1|1.9e-6663.02PREDICTED: uncharacterized protein LOC106799045, partial [Glycine max][more]
gi|955333235|ref|XP_014633953.1|1.9e-6664.06PREDICTED: uncharacterized protein LOC106799574, partial [Glycine max][more]
gi|955353768|ref|XP_014620239.1|1.9e-6663.54PREDICTED: uncharacterized protein LOC106795329, partial [Glycine max][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004523RNA-DNA hybrid ribonuclease activity
GO:0003676nucleic acid binding
Vocabulary: INTERPRO
TermDefinition
IPR012337RNaseH-like_sf
IPR002156RNaseH_domain
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051252 regulation of RNA metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi06G007850.1Lsi06G007850.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPROFILEPS50879RNASE_Hcoord: 74..189
score: 11
IPR012337Ribonuclease H-like domainGENE3DG3DSA:3.30.420.10coord: 79..187
score: 4.
IPR012337Ribonuclease H-like domainunknownSSF53098Ribonuclease H-likecoord: 76..188
score: 8.35
NoneNo IPR availablePANTHERPTHR33033FAMILY NOT NAMEDcoord: 2..56
score: 6.3E-26coord: 78..189
score: 6.3
NoneNo IPR availablePANTHERPTHR33033:SF10SUBFAMILY NOT NAMEDcoord: 2..56
score: 6.3E-26coord: 78..189
score: 6.3
NoneNo IPR availablePFAMPF13456RVT_3coord: 82..189
score: 4.5

Synteny
The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Lsi06G007850Wax gourdlsiwgoB521
Lsi06G007850Wax gourdlsiwgoB531
Lsi06G007850Cucumber (Gy14) v1cgylsiB473
Lsi06G007850Cucumber (Gy14) v1cgylsiB474
Lsi06G007850Cucurbita maxima (Rimu)cmalsiB078
Lsi06G007850Cucurbita maxima (Rimu)cmalsiB128
Lsi06G007850Cucurbita moschata (Rifu)cmolsiB064
Lsi06G007850Cucurbita moschata (Rifu)cmolsiB118
Lsi06G007850Cucurbita pepo (Zucchini)cpelsiB559
Lsi06G007850Cucurbita pepo (Zucchini)cpelsiB627
Lsi06G007850Wild cucumber (PI 183967)cpilsiB059
Lsi06G007850Cucumber (Chinese Long) v2culsiB058
Lsi06G007850Watermelon (Charleston Gray)lsiwcgB375
Lsi06G007850Watermelon (Charleston Gray)lsiwcgB377
Lsi06G007850Watermelon (97103) v1lsiwmB419
Lsi06G007850Cucumber (Gy14) v2cgyblsiB055
Lsi06G007850Silver-seed gourdcarlsiB022
Lsi06G007850Silver-seed gourdcarlsiB830
Lsi06G007850Cucumber (Chinese Long) v3cuclsiB068
Lsi06G007850Watermelon (97103) v2lsiwmbB378