Lsi06G006960 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi06G006960
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Adipose-regulatory protein (Seipin), putative
Location	chr06 : 11056237 .. 11056980 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATTATCTAAACTCAATATTTGCTGATCATCAGTTGAGTGCAGAGTTGATATCAACAAATGGAAACATAATAACCGGTTCAAGCCAACCCTGCATGCTCCGGTTCAGAAGCACACCGGTCCGGTTGACCCGAACTTTTCTCACTAGCTTTCCTCTCCTCTTAGGAATCTCAAGTGAATCCCAAAGGCTAACTTTTCCCATACTAGAGCACAAGGAAGAAAATCACCAACGAAGTGCAGCCATCCATGTTACTATTAGCCCTCGCATTGGAACCACGGCCCTCCCCGAGCTCTACGAAGCTCATATTTTAATAAACTCGAAACTACCAAAGACGAAAGAGTTGTTGCATCGGTGGCGATGGACTTGCTTCCTATGGATGTCTCTTTATTTGTACGTGATGTTTCTAGTGATCTTTATGTTCTTTTGGAAGCCGGTTGTGTTTCGTGCAATGACGTTGTCGGAGTTGAGTGGTTTTGATGAAGGAGCTCCGACGACGAAGGAAGCAGAGGAGTCGTTCGATGAGATGGCGGAGATAACGGTGGAGTTGTTGAGAAAATGGCAAGAGATAAGGAGGAAGAGAAAGGCTGCCGTGTTTGGGTATGGAGAAGTTGGAGGAGGAGAAGATGACGTTGGGTCGACATCTGCGTCGAGCATTAGTTGTAGCAGAGAGTATGTAGGTGCTGTTTTCGAGGAAGATGTCGAGGACTCGGAGTCGGTGCTTCTAGAAGGTTCGGAGGAGTAG

mRNA sequence

ATGAATTATCTAAACTCAATATTTGCTGATCATCAGTTGAGTGCAGAGTTGATATCAACAAATGGAAACATAATAACCGGTTCAAGCCAACCCTGCATGCTCCGGTTCAGAAGCACACCGGTCCGGTTGACCCGAACTTTTCTCACTAGCTTTCCTCTCCTCTTAGGAATCTCAAGTGAATCCCAAAGGCTAACTTTTCCCATACTAGAGCACAAGGAAGAAAATCACCAACGAAGTGCAGCCATCCATGTTACTATTAGCCCTCGCATTGGAACCACGGCCCTCCCCGAGCTCTACGAAGCTCATATTTTAATAAACTCGAAACTACCAAAGACGAAAGAGTTGTTGCATCGGTGGCGATGGACTTGCTTCCTATGGATGTCTCTTTATTTGTACGTGATGTTTCTAGTGATCTTTATGTTCTTTTGGAAGCCGGTTGTGTTTCGTGCAATGACGTTGTCGGAGTTGAGTGGTTTTGATGAAGGAGCTCCGACGACGAAGGAAGCAGAGGAGTCGTTCGATGAGATGGCGGAGATAACGGTGGAGTTGTTGAGAAAATGGCAAGAGATAAGGAGGAAGAGAAAGGCTGCCGTGTTTGGGTATGGAGAAGTTGGAGGAGGAGAAGATGACGTTGGGTCGACATCTGCGTCGAGCATTAGTTGTAGCAGAGAGTATGTAGGTGCTGTTTTCGAGGAAGATGTCGAGGACTCGGAGTCGGTGCTTCTAGAAGGTTCGGAGGAGTAG

Coding sequence (CDS)

ATGAATTATCTAAACTCAATATTTGCTGATCATCAGTTGAGTGCAGAGTTGATATCAACAAATGGAAACATAATAACCGGTTCAAGCCAACCCTGCATGCTCCGGTTCAGAAGCACACCGGTCCGGTTGACCCGAACTTTTCTCACTAGCTTTCCTCTCCTCTTAGGAATCTCAAGTGAATCCCAAAGGCTAACTTTTCCCATACTAGAGCACAAGGAAGAAAATCACCAACGAAGTGCAGCCATCCATGTTACTATTAGCCCTCGCATTGGAACCACGGCCCTCCCCGAGCTCTACGAAGCTCATATTTTAATAAACTCGAAACTACCAAAGACGAAAGAGTTGTTGCATCGGTGGCGATGGACTTGCTTCCTATGGATGTCTCTTTATTTGTACGTGATGTTTCTAGTGATCTTTATGTTCTTTTGGAAGCCGGTTGTGTTTCGTGCAATGACGTTGTCGGAGTTGAGTGGTTTTGATGAAGGAGCTCCGACGACGAAGGAAGCAGAGGAGTCGTTCGATGAGATGGCGGAGATAACGGTGGAGTTGTTGAGAAAATGGCAAGAGATAAGGAGGAAGAGAAAGGCTGCCGTGTTTGGGTATGGAGAAGTTGGAGGAGGAGAAGATGACGTTGGGTCGACATCTGCGTCGAGCATTAGTTGTAGCAGAGAGTATGTAGGTGCTGTTTTCGAGGAAGATGTCGAGGACTCGGAGTCGGTGCTTCTAGAAGGTTCGGAGGAGTAG

Protein sequence

MNYLNSIFADHQLSAELISTNGNIITGSSQPCMLRFRSTPVRLTRTFLTSFPLLLGISSESQRLTFPILEHKEENHQRSAAIHVTISPRIGTTALPELYEAHILINSKLPKTKELLHRWRWTCFLWMSLYLYVMFLVIFMFFWKPVVFRAMTLSELSGFDEGAPTTKEAEESFDEMAEITVELLRKWQEIRRKRKAAVFGYGEVGGGEDDVGSTSASSISCSREYVGAVFEEDVEDSESVLLEGSEE

Homology

[Close]

BLAST of Lsi06G006960 vs. Swiss-Prot
Match: SEI1_ARATH (Seipin-1 OS=Arabidopsis thaliana GN=SEI1 PE=2 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 9.2e-29
Identity = 76/184 (41.30%), Postives = 106/184 (57.61%), Query Frame = 1


Query: 12  QLSAELISTNGNIITGSSQPCMLRFRSTPVRLTRTFLTSFPLLLGISSESQRLTFPILEH 71
           QL  EL+S  G  I  SSQPCMLRFRS P+RL RTF+ S PL+ GI++E+Q +    L+H
Sbjct: 182 QLKVELLSLKGETIARSSQPCMLRFRSKPIRLARTFVMSVPLIAGIANEAQTMRIDALKH 241

Query: 72  KEENHQRSAAIHVTISPRIGTTALPELYEAHILINSKLPKTKELLHRWRWTCFLWMSLYL 131
           +E+   R+ A+  T+ PR  T  LP+LYEA I+INSK P  K + + W+WT  +W S+YL
Sbjct: 242 QEKM-PRTKAVRATLIPRAQTRTLPQLYEAEIVINSKPPWIKRMAYNWKWTLCVWTSMYL 301

Query: 132 YVMFLVIFMFFWKPVVFRAMTLSELSGFDEGAPTTKEAEESFDEMAEITVELLRKWQEIR 191
           YV  L   ++ ++PV+F               P T     S  E  EI V    + Q + 
Sbjct: 302 YVAILTALLWCFRPVLF---------------PYTSSRTISESENLEIEVVEEEQEQVME 349

Query: 192 RKRK 196
           R+R+
Sbjct: 362 RRRR 349

[Close]

BLAST of Lsi06G006960 vs. Swiss-Prot
Match: SEI3_ARATH (Seipin-3 OS=Arabidopsis thaliana GN=SEI3 PE=2 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 5.2e-16
Identity = 45/139 (32.37%), Postives = 82/139 (58.99%), Query Frame = 1


Query: 12  QLSAELISTNGNIITGSSQPCMLRFRSTPVRLTRTFLTSFPLLLGISSESQRLTFPILEH 71
           Q+  + +S +G+++  S +PCM++F S P+RL +T L   PL+ G  SE Q L   +   
Sbjct: 341 QVRVDFLSASGHVLASSRRPCMVKFSSEPIRLVQTLLKIAPLVTGYVSEIQTLNLKLKGL 400

Query: 72  KEENHQRSAAIHVTISPRIGT---TALPELYEAHILINSKLPKTKELLHRWRWTCFLWMS 131
            E++   +A + + I  R        +PE+Y+A + + SKLP  K ++  WR T F+W+S
Sbjct: 401 VEKDIIPTACLKIMIEQRAEFRPGAGIPEIYDASLFLESKLPFLKRIIWNWRKTLFVWIS 460

Query: 132 LYLYVMFLVIFMFFWKPVV 148
           + L++M L+  + F++P++
Sbjct: 461 MSLFIMELLFALVFFRPLI 479

[Close]

BLAST of Lsi06G006960 vs. Swiss-Prot
Match: SEI2_ARATH (Seipin-2 OS=Arabidopsis thaliana GN=SEI2 PE=2 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 2.4e-13
Identity = 45/139 (32.37%), Postives = 79/139 (56.83%), Query Frame = 1


Query: 12  QLSAELISTNGNIITGSSQPCMLRFRSTPVRLTRTFLTSFPLLLGISSESQRLTFPILEH 71
           Q+  + +S +G  I    +PCMLRFRS P+RL +TF    PL+ G  SE Q L+  +   
Sbjct: 370 QVRVDFLSVDGQTIASIRRPCMLRFRSEPIRLVQTFFKVVPLVTGYVSEIQTLSLKLKGF 429

Query: 72  KEENHQRSAAIHVTISPRI---GTTALPELYEAHILINSKLPKTKELLHRWRWTCFLWMS 131
            E++   +A + + I  R        +PELY+A + + S LP  ++++ +WR T F+W+S
Sbjct: 430 VEKDIP-TACLKIIIEQRAEFRPGAGIPELYDASLSVESGLPFFRKIIWKWRKTLFVWIS 489

Query: 132 LYLYVMFLVIFMFFWKPVV 148
           + L++  L+  +   +P++
Sbjct: 490 MSLFITELLFTLVCCRPLI 507

[Close]

BLAST of Lsi06G006960 vs. TrEMBL
Match: A0A0A0M367_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G657470 PE=4 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 5.5e-89
Identity = 181/247 (73.28%), Postives = 204/247 (82.59%), Query Frame = 1


Query: 5   NSIFADHQLSAELISTNGNIITGSSQPCMLRFRSTPVRLTRTFLTSFPLLLGISSESQRL 64
           N  F   QLSAELISTNGN+IT SSQPCMLRFRS PVRL RT +TSFP+L+GISSE+QRL
Sbjct: 181 NREFGVFQLSAELISTNGNVITSSSQPCMLRFRSAPVRLARTIITSFPVLVGISSEAQRL 240

Query: 65  TFPILEHKEENHQRSAAIHVTISPRIGTTALPELYEAHILINSKLPKTKELLHRWRWTCF 124
           +FPILEHKEE  +RSAAI VTISPRIGT+ALPELYEA ILINSK PK KELL RW+WTCF
Sbjct: 241 SFPILEHKEEKQERSAAIQVTISPRIGTSALPELYEADILINSKPPKMKELLRRWQWTCF 300

Query: 125 LWMSLYLYVMFLVIFMFFWKPVVFRAMTLSE---LSGFDEGAPTTKEAE---ESFDEMAE 184
           +W S+YLY+MF+V+FMFFWKPV+FRAMTL     +   D+  P  +E E    S DEMAE
Sbjct: 301 VWTSMYLYLMFVVMFMFFWKPVMFRAMTLRPHQLIRDLDQD-PRRREVEGDESSLDEMAE 360

Query: 185 ITVELLRKWQEIRRKRKAAVFGYGEVGGGEDDVGSTSASSISCSREYVGAVFEEDVEDSE 244
           ITVELLRKWQE+RRKRKAA+FGYG    GE+DVGSTSASSISCSR+Y  AVFEEDV DSE
Sbjct: 361 ITVELLRKWQEMRRKRKAAMFGYGS---GEEDVGSTSASSISCSRDYTAAVFEEDVGDSE 420

Query: 245 SVLLEGS 246
           SV+LE S
Sbjct: 421 SVILEAS 423

[Close]

BLAST of Lsi06G006960 vs. TrEMBL
Match: D7SPQ8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_04s0023g00480 PE=4 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 5.8e-46
Identity = 116/231 (50.22%), Postives = 156/231 (67.53%), Query Frame = 1


Query: 12  QLSAELISTNGNIITGSSQPCMLRFRSTPVRLTRTFLTSFPLLLGISSESQRLTFPILEH 71
           QL+AE++S NG++I  SS PCMLRFRS PV+LTRTFL   PLLLGISSE+Q LT  IL+H
Sbjct: 30  QLTAEVMSINGDVIAKSSHPCMLRFRSLPVQLTRTFLMGVPLLLGISSETQELTIEILKH 89

Query: 72  KEENHQRSAAIHVTISPRIGTTALPELYEAHILINSKLPKTKELLHRWRWTCFLWMSLYL 131
           KE  + R+ AI VT+ PR GT +LP++YEA +L+NS+LP  K+L+H W+WT ++WMSLY+
Sbjct: 90  KE-GYSRTKAIRVTLIPRAGTLSLPQIYEAELLMNSQLPWKKKLVHSWKWTFYVWMSLYV 149

Query: 132 YVMFLVIFMFFWKPVVFRAMTLSEL--SGFDEGAPTTKEAEESFDEMAEITVELLRKWQE 191
           Y+M LV+ M  ++PV+F   T S+   +  D     +KE +    +  E + E L++WQ 
Sbjct: 150 YIMLLVVLMCCFRPVLFPVTTASQRDHNERDLTMEVSKEPDSRARDAREAS-ESLKRWQR 209

Query: 192 IRRKRKAAVFGYGEVGGGEDDVGSTSASSISCSREYVGAVFEEDVEDSESV 241
            R KRK  V    +     + VGS SASSI+ +RE   A  EEDV DSESV
Sbjct: 210 SRNKRKVDVLQKLQ----PETVGS-SASSITITREDTSAFVEEDVGDSESV 253

[Close]

BLAST of Lsi06G006960 vs. TrEMBL
Match: A0A061EZL5_THECC (Adipose-regulatory protein, putative OS=Theobroma cacao GN=TCM_025247 PE=4 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 3.7e-45
Identity = 114/237 (48.10%), Postives = 156/237 (65.82%), Query Frame = 1


Query: 12  QLSAELISTNGNIITGSSQPCMLRFRSTPVRLTRTFLTSFPLLLGISSESQRLTFPILEH 71
           QL+AEL+S+NGN+I  SSQP ML FRS PVRL RTFL   PLLLGISSE+Q++   IL +
Sbjct: 188 QLTAELLSSNGNVIAKSSQPVMLWFRSLPVRLARTFLMGLPLLLGISSETQKIKIEILRY 247

Query: 72  KEENHQRSAAIHVTISPRIGTTALPELYEAHILINSKLPKTKELLHRWRWTCFLWMSLYL 131
           KE  H R+ A+ V ++PR GT+ LP+LY+A I++NS+LP  K+LL  W+WT ++W SL++
Sbjct: 248 KE-GHPRTEAVRVILAPRAGTSLLPQLYDAQIIMNSQLPWNKQLLRNWKWTLYVWTSLHV 307

Query: 132 YVMFLVIFMFFWKPVVFRAMTLSELSGFDEGAPTTKEAEESF----DEMAEITVELLRKW 191
           Y+MFLVI +   KP+ F  +T +  S  DEG    +E+ E      ++ +E++ +LL KW
Sbjct: 308 YIMFLVILIGCCKPLFFPVIT-AGFSNRDEGEAGEEESIEPVISGRNDQSEVS-DLLSKW 367

Query: 192 QEIRRKRKAAVFGYGEVGGGEDDVGSTSASSISCSREYVGAVFEEDVEDSESVLLEG 245
              RRK KA       +     D+  +SASS+S +RE    V EEDV DSESV L G
Sbjct: 368 YHSRRKHKATF-----LHKNFSDIAGSSASSMSVTREDTSGVIEEDVGDSESVCLGG 416

[Close]

BLAST of Lsi06G006960 vs. TrEMBL
Match: A5BSZ7_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_024107 PE=4 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 6.4e-45
Identity = 115/231 (49.78%), Postives = 155/231 (67.10%), Query Frame = 1


Query: 12  QLSAELISTNGNIITGSSQPCMLRFRSTPVRLTRTFLTSFPLLLGISSESQRLTFPILEH 71
           QL+AE++S NG++I  SS PCMLRFRS PV+LTRTFL   PLLLGISSE+Q LT  IL+H
Sbjct: 179 QLTAEVMSINGDVIAKSSHPCMLRFRSLPVQLTRTFLMGVPLLLGISSETQELTIEILKH 238

Query: 72  KEENHQRSAAIHVTISPRIGTTALPELYEAHILINSKLPKTKELLHRWRWTCFLWMSLYL 131
           KE  + R+ AI VT+ PR GT +LP++YEA +L+NS+LP  K+L+  W+WT ++WMSLY+
Sbjct: 239 KE-GYSRTKAIRVTLIPRAGTLSLPQIYEAELLMNSQLPWKKKLVXSWKWTFYVWMSLYV 298

Query: 132 YVMFLVIFMFFWKPVVFRAMTLSEL--SGFDEGAPTTKEAEESFDEMAEITVELLRKWQE 191
           Y+M LV+ M  ++PV+F   T S+   +  D     +KE +    +  E + E L++WQ 
Sbjct: 299 YIMLLVVLMCCFRPVLFPVTTASQRDHNERDLTMEVSKEPDSRARDAREAS-ESLKRWQR 358

Query: 192 IRRKRKAAVFGYGEVGGGEDDVGSTSASSISCSREYVGAVFEEDVEDSESV 241
            R KRK  V    +     + VGS SASSI+ +RE   A  EEDV DSESV
Sbjct: 359 SRNKRKVDVLQKLQ----PETVGS-SASSITITREDTSAFVEEDVGDSESV 402

[Close]

BLAST of Lsi06G006960 vs. TrEMBL
Match: I1L6A3_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_09G250400 PE=4 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 8.3e-45
Identity = 111/232 (47.84%), Postives = 157/232 (67.67%), Query Frame = 1


Query: 12  QLSAELISTNGNIITGSSQPCMLRFRSTPVRLTRTFLTSFPLLLGISSESQRLTFPILEH 71
           QL+AELIS NGN+I  SSQPCMLRFRS+P+RL RTF+   PL+LGIS E+Q +   IL H
Sbjct: 182 QLTAELISVNGNVIAKSSQPCMLRFRSSPIRLARTFMMGVPLVLGISGETQNINVEILRH 241

Query: 72  KEENHQRSAAIHVTISPRIGTTALPELYEAHILINSKLPKTKELLHRWRWTCFLWMSLYL 131
           KE+ ++RS AI VT+ PR GT++LP+LYEA I+I S LP TKEL+  W+WT ++W+SLY+
Sbjct: 242 KED-YRRSNAIRVTLHPRAGTSSLPQLYEADIVIKSHLPWTKELVRNWKWTFYVWVSLYV 301

Query: 132 YVMFLVIFMFFWKPVVFRAMTLSELSGFDEGAPTTKEAEESFDEMAEITVELLRKWQEIR 191
           Y++ LV  +  ++P+VF  +   E       +  T+E      + +E++ ELLRKW+  R
Sbjct: 302 YIVLLVSLLCCYRPLVF--LVTPEYFNDHRVSELTREEPGQLGDESEVS-ELLRKWRRSR 361

Query: 192 RKRKAAVFGYGEVGGGEDDVGSTSAS-SISCSREYVGAVFEEDVEDSESVLL 243
            KRK  V  +G  G  E  VGS+++S S++   +    V E++VEDSESV +
Sbjct: 362 SKRK-TVLAHG--GVPETIVGSSASSISMTAREDVTSVVMEDEVEDSESVCI 406

[Close]

BLAST of Lsi06G006960 vs. TAIR10
Match: AT5G16460.1 (AT5G16460.1 Putative adipose-regulatory protein (Seipin))

HSP 1 Score: 128.6 bits (322), Expect = 5.2e-30
Identity = 76/184 (41.30%), Postives = 106/184 (57.61%), Query Frame = 1


Query: 12  QLSAELISTNGNIITGSSQPCMLRFRSTPVRLTRTFLTSFPLLLGISSESQRLTFPILEH 71
           QL  EL+S  G  I  SSQPCMLRFRS P+RL RTF+ S PL+ GI++E+Q +    L+H
Sbjct: 182 QLKVELLSLKGETIARSSQPCMLRFRSKPIRLARTFVMSVPLIAGIANEAQTMRIDALKH 241

Query: 72  KEENHQRSAAIHVTISPRIGTTALPELYEAHILINSKLPKTKELLHRWRWTCFLWMSLYL 131
           +E+   R+ A+  T+ PR  T  LP+LYEA I+INSK P  K + + W+WT  +W S+YL
Sbjct: 242 QEKM-PRTKAVRATLIPRAQTRTLPQLYEAEIVINSKPPWIKRMAYNWKWTLCVWTSMYL 301

Query: 132 YVMFLVIFMFFWKPVVFRAMTLSELSGFDEGAPTTKEAEESFDEMAEITVELLRKWQEIR 191
           YV  L   ++ ++PV+F               P T     S  E  EI V    + Q + 
Sbjct: 302 YVAILTALLWCFRPVLF---------------PYTSSRTISESENLEIEVVEEEQEQVME 349

Query: 192 RKRK 196
           R+R+
Sbjct: 362 RRRR 349

[Close]

BLAST of Lsi06G006960 vs. TAIR10
Match: AT2G34380.1 (AT2G34380.1 Putative adipose-regulatory protein (Seipin))

HSP 1 Score: 86.3 bits (212), Expect = 2.9e-17
Identity = 45/139 (32.37%), Postives = 82/139 (58.99%), Query Frame = 1


Query: 12  QLSAELISTNGNIITGSSQPCMLRFRSTPVRLTRTFLTSFPLLLGISSESQRLTFPILEH 71
           Q+  + +S +G+++  S +PCM++F S P+RL +T L   PL+ G  SE Q L   +   
Sbjct: 341 QVRVDFLSASGHVLASSRRPCMVKFSSEPIRLVQTLLKIAPLVTGYVSEIQTLNLKLKGL 400

Query: 72  KEENHQRSAAIHVTISPRIGT---TALPELYEAHILINSKLPKTKELLHRWRWTCFLWMS 131
            E++   +A + + I  R        +PE+Y+A + + SKLP  K ++  WR T F+W+S
Sbjct: 401 VEKDIIPTACLKIMIEQRAEFRPGAGIPEIYDASLFLESKLPFLKRIIWNWRKTLFVWIS 460

Query: 132 LYLYVMFLVIFMFFWKPVV 148
           + L++M L+  + F++P++
Sbjct: 461 MSLFIMELLFALVFFRPLI 479

[Close]

BLAST of Lsi06G006960 vs. TAIR10
Match: AT1G29760.1 (AT1G29760.1 Putative adipose-regulatory protein (Seipin))

HSP 1 Score: 77.4 bits (189), Expect = 1.4e-14
Identity = 45/139 (32.37%), Postives = 79/139 (56.83%), Query Frame = 1


Query: 12  QLSAELISTNGNIITGSSQPCMLRFRSTPVRLTRTFLTSFPLLLGISSESQRLTFPILEH 71
           Q+  + +S +G  I    +PCMLRFRS P+RL +TF    PL+ G  SE Q L+  +   
Sbjct: 370 QVRVDFLSVDGQTIASIRRPCMLRFRSEPIRLVQTFFKVVPLVTGYVSEIQTLSLKLKGF 429

Query: 72  KEENHQRSAAIHVTISPRI---GTTALPELYEAHILINSKLPKTKELLHRWRWTCFLWMS 131
            E++   +A + + I  R        +PELY+A + + S LP  ++++ +WR T F+W+S
Sbjct: 430 VEKDIP-TACLKIIIEQRAEFRPGAGIPELYDASLSVESGLPFFRKIIWKWRKTLFVWIS 489

Query: 132 LYLYVMFLVIFMFFWKPVV 148
           + L++  L+  +   +P++
Sbjct: 490 MSLFITELLFTLVCCRPLI 507

[Close]

BLAST of Lsi06G006960 vs. NCBI nr
Match: gi|659085951|ref|XP_008443693.1| (PREDICTED: uncharacterized protein LOC103487228 [Cucumis melo])

HSP 1 Score: 356.7 bits (914), Expect = 3.3e-95
Identity = 191/247 (77.33%), Postives = 213/247 (86.23%), Query Frame = 1


Query: 5   NSIFADHQLSAELISTNGNIITGSSQPCMLRFRSTPVRLTRTFLTSFPLLLGISSESQRL 64
           N  F   QLSAELISTNGNIIT SSQPCMLRFRS PVR  RT LTSFP+L+GIS+E+QRL
Sbjct: 183 NREFGVFQLSAELISTNGNIITSSSQPCMLRFRSAPVRFARTLLTSFPILIGISTEAQRL 242

Query: 65  TFPILEHKEENHQRSAAIHVTISPRIGTTALPELYEAHILINSKLPKTKELLHRWRWTCF 124
           +FPIL+HKEEN +RSAAI  TISPRIGT+ALPELYEAHI+INSKLP+ KELL RWRWTCF
Sbjct: 243 SFPILKHKEENQERSAAIQFTISPRIGTSALPELYEAHIVINSKLPRMKELLRRWRWTCF 302

Query: 125 LWMSLYLYVMFLVIFMFFWKPVVFRAMTLS-ELSGFDEGAPTTKE---AEESFDEMAEIT 184
           LW S+YLY+MFL IFM FWKPV+FRAMTL+ ELS FD+  P  +E   A+ES DEMAEIT
Sbjct: 303 LWTSIYLYLMFLAIFMLFWKPVMFRAMTLTPELSDFDQD-PRRRELEGADESLDEMAEIT 362

Query: 185 VELLRKWQEIRRKRKAAVFGYGEVGGGEDDVGSTSASSISCSREYVGAVFEEDVEDSESV 244
           VELLRKWQE+RRKRKAA+FGY   G GE+DVGSTSASSISCSR+Y+ AVFEEDV DSESV
Sbjct: 363 VELLRKWQEMRRKRKAAMFGY---GSGEEDVGSTSASSISCSRDYIAAVFEEDVGDSESV 422

Query: 245 LLEGSEE 248
           LLEGSEE
Sbjct: 423 LLEGSEE 425

[Close]

BLAST of Lsi06G006960 vs. NCBI nr
Match: gi|449442797|ref|XP_004139167.1| (PREDICTED: uncharacterized protein LOC101206366 [Cucumis sativus])

HSP 1 Score: 335.5 bits (859), Expect = 7.9e-89
Identity = 181/247 (73.28%), Postives = 204/247 (82.59%), Query Frame = 1


Query: 5   NSIFADHQLSAELISTNGNIITGSSQPCMLRFRSTPVRLTRTFLTSFPLLLGISSESQRL 64
           N  F   QLSAELISTNGN+IT SSQPCMLRFRS PVRL RT +TSFP+L+GISSE+QRL
Sbjct: 181 NREFGVFQLSAELISTNGNVITSSSQPCMLRFRSAPVRLARTIITSFPVLVGISSEAQRL 240

Query: 65  TFPILEHKEENHQRSAAIHVTISPRIGTTALPELYEAHILINSKLPKTKELLHRWRWTCF 124
           +FPILEHKEE  +RSAAI VTISPRIGT+ALPELYEA ILINSK PK KELL RW+WTCF
Sbjct: 241 SFPILEHKEEKQERSAAIQVTISPRIGTSALPELYEADILINSKPPKMKELLRRWQWTCF 300

Query: 125 LWMSLYLYVMFLVIFMFFWKPVVFRAMTLSE---LSGFDEGAPTTKEAE---ESFDEMAE 184
           +W S+YLY+MF+V+FMFFWKPV+FRAMTL     +   D+  P  +E E    S DEMAE
Sbjct: 301 VWTSMYLYLMFVVMFMFFWKPVMFRAMTLRPHQLIRDLDQD-PRRREVEGDESSLDEMAE 360

Query: 185 ITVELLRKWQEIRRKRKAAVFGYGEVGGGEDDVGSTSASSISCSREYVGAVFEEDVEDSE 244
           ITVELLRKWQE+RRKRKAA+FGYG    GE+DVGSTSASSISCSR+Y  AVFEEDV DSE
Sbjct: 361 ITVELLRKWQEMRRKRKAAMFGYGS---GEEDVGSTSASSISCSRDYTAAVFEEDVGDSE 420

Query: 245 SVLLEGS 246
           SV+LE S
Sbjct: 421 SVILEAS 423

[Close]

BLAST of Lsi06G006960 vs. NCBI nr
Match: gi|645223055|ref|XP_008218447.1| (PREDICTED: uncharacterized protein LOC103318793 [Prunus mume])

HSP 1 Score: 195.7 bits (496), Expect = 9.8e-47
Identity = 122/249 (49.00%), Postives = 159/249 (63.86%), Query Frame = 1


Query: 12  QLSAELISTNGNIITGSSQPCMLRFRSTPVRLTRTFLTSFPLLLGISSESQRLTFPILEH 71
           Q+SAEL+S NG++I  SSQPCMLRFRS PVRLTRTFL   PLLLGIS E+Q+LT  ILEH
Sbjct: 198 QMSAELLSVNGDLIAKSSQPCMLRFRSLPVRLTRTFLMGVPLLLGISGETQKLTMQILEH 257

Query: 72  KEENHQRSAAIHVTISPRIGTTALPELYEAHILINSKLPKTKELLHRWRWTCFLWMSLYL 131
           KE +  R+ AI VT+ PR GT+ LP+LYEA IL+NS+LP TK L+  W+ T ++W SLY 
Sbjct: 258 KEGHFPRTQAIRVTLLPRAGTSYLPQLYEAEILLNSQLPWTKHLVRSWKLTFYVWTSLYT 317

Query: 132 YVMFLVIFMFFWKPVVF--------RAMTLSELSGFDEGAPTT---------KEAEESFD 191
           YV+FL+I + F KP++          A  +S+ S  +  +  T         +    S D
Sbjct: 318 YVLFLIILILFCKPLLLPMTMITATAAANVSDQSYTETQSQATSRPRRRHSRESQASSRD 377

Query: 192 EMAEITVELLRKWQEIRRKRKAAVFGYGEVG-GGEDDVGSTSASSISCSREYVGAVFEED 243
             +E   ELLRKWQ  R KRKA     G++  G  + +GS++AS+IS +RE   +V  ED
Sbjct: 378 VESEDVQELLRKWQRSRSKRKAMYLQPGDLDLGAAETIGSSAASTISITRE-DASVATED 437

[Close]

BLAST of Lsi06G006960 vs. NCBI nr
Match: gi|297735275|emb|CBI17637.3| (unnamed protein product [Vitis vinifera])

HSP 1 Score: 192.6 bits (488), Expect = 8.3e-46
Identity = 116/231 (50.22%), Postives = 156/231 (67.53%), Query Frame = 1


Query: 12  QLSAELISTNGNIITGSSQPCMLRFRSTPVRLTRTFLTSFPLLLGISSESQRLTFPILEH 71
           QL+AE++S NG++I  SS PCMLRFRS PV+LTRTFL   PLLLGISSE+Q LT  IL+H
Sbjct: 30  QLTAEVMSINGDVIAKSSHPCMLRFRSLPVQLTRTFLMGVPLLLGISSETQELTIEILKH 89

Query: 72  KEENHQRSAAIHVTISPRIGTTALPELYEAHILINSKLPKTKELLHRWRWTCFLWMSLYL 131
           KE  + R+ AI VT+ PR GT +LP++YEA +L+NS+LP  K+L+H W+WT ++WMSLY+
Sbjct: 90  KE-GYSRTKAIRVTLIPRAGTLSLPQIYEAELLMNSQLPWKKKLVHSWKWTFYVWMSLYV 149

Query: 132 YVMFLVIFMFFWKPVVFRAMTLSEL--SGFDEGAPTTKEAEESFDEMAEITVELLRKWQE 191
           Y+M LV+ M  ++PV+F   T S+   +  D     +KE +    +  E + E L++WQ 
Sbjct: 150 YIMLLVVLMCCFRPVLFPVTTASQRDHNERDLTMEVSKEPDSRARDAREAS-ESLKRWQR 209

Query: 192 IRRKRKAAVFGYGEVGGGEDDVGSTSASSISCSREYVGAVFEEDVEDSESV 241
            R KRK  V    +     + VGS SASSI+ +RE   A  EEDV DSESV
Sbjct: 210 SRNKRKVDVLQKLQ----PETVGS-SASSITITREDTSAFVEEDVGDSESV 253

[Close]

BLAST of Lsi06G006960 vs. NCBI nr
Match: gi|225430975|ref|XP_002272148.1| (PREDICTED: uncharacterized protein LOC100263320 [Vitis vinifera])

HSP 1 Score: 192.6 bits (488), Expect = 8.3e-46
Identity = 116/231 (50.22%), Postives = 156/231 (67.53%), Query Frame = 1


Query: 12  QLSAELISTNGNIITGSSQPCMLRFRSTPVRLTRTFLTSFPLLLGISSESQRLTFPILEH 71
           QL+AE++S NG++I  SS PCMLRFRS PV+LTRTFL   PLLLGISSE+Q LT  IL+H
Sbjct: 179 QLTAEVMSINGDVIAKSSHPCMLRFRSLPVQLTRTFLMGVPLLLGISSETQELTIEILKH 238

Query: 72  KEENHQRSAAIHVTISPRIGTTALPELYEAHILINSKLPKTKELLHRWRWTCFLWMSLYL 131
           KE  + R+ AI VT+ PR GT +LP++YEA +L+NS+LP  K+L+H W+WT ++WMSLY+
Sbjct: 239 KE-GYSRTKAIRVTLIPRAGTLSLPQIYEAELLMNSQLPWKKKLVHSWKWTFYVWMSLYV 298

Query: 132 YVMFLVIFMFFWKPVVFRAMTLSEL--SGFDEGAPTTKEAEESFDEMAEITVELLRKWQE 191
           Y+M LV+ M  ++PV+F   T S+   +  D     +KE +    +  E + E L++WQ 
Sbjct: 299 YIMLLVVLMCCFRPVLFPVTTASQRDHNERDLTMEVSKEPDSRARDAREAS-ESLKRWQR 358

Query: 192 IRRKRKAAVFGYGEVGGGEDDVGSTSASSISCSREYVGAVFEEDVEDSESV 241
            R KRK  V    +     + VGS SASSI+ +RE   A  EEDV DSESV
Sbjct: 359 SRNKRKVDVLQKLQ----PETVGS-SASSITITREDTSAFVEEDVGDSESV 402

The following BLAST results are available for this feature:

BLAST of Lsi06G006960 vs. Swiss-Prot


Match Name	E-value	Identity	Description
SEI1_ARATH	9.2e-29	41.30	Seipin-1 OS=Arabidopsis thaliana GN=SEI1 PE=2 SV=1	[more]
SEI3_ARATH	5.2e-16	32.37	Seipin-3 OS=Arabidopsis thaliana GN=SEI3 PE=2 SV=1	[more]
SEI2_ARATH	2.4e-13	32.37	Seipin-2 OS=Arabidopsis thaliana GN=SEI2 PE=2 SV=1	[more]

BLAST of Lsi06G006960 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0M367_CUCSA	5.5e-89	73.28	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G657470 PE=4 SV=1	[more]
D7SPQ8_VITVI	5.8e-46	50.22	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_04s0023g00480 PE=4 SV=...	[more]
A0A061EZL5_THECC	3.7e-45	48.10	Adipose-regulatory protein, putative OS=Theobroma cacao GN=TCM_025247 PE=4 SV=1	[more]
A5BSZ7_VITVI	6.4e-45	49.78	Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_024107 PE=4 SV=1	[more]
I1L6A3_SOYBN	8.3e-45	47.84	Uncharacterized protein OS=Glycine max GN=GLYMA_09G250400 PE=4 SV=1	[more]

BLAST of Lsi06G006960 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G16460.1	5.2e-30	41.30	Putative adipose-regulatory protein (Seipin)	[more]
AT2G34380.1	2.9e-17	32.37	Putative adipose-regulatory protein (Seipin)	[more]
AT1G29760.1	1.4e-14	32.37	Putative adipose-regulatory protein (Seipin)	[more]

BLAST of Lsi06G006960 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659085951\|ref\|XP_008443693.1\|	3.3e-95	77.33	PREDICTED: uncharacterized protein LOC103487228 [Cucumis melo]	[more]
gi\|449442797\|ref\|XP_004139167.1\|	7.9e-89	73.28	PREDICTED: uncharacterized protein LOC101206366 [Cucumis sativus]	[more]
gi\|645223055\|ref\|XP_008218447.1\|	9.8e-47	49.00	PREDICTED: uncharacterized protein LOC103318793 [Prunus mume]	[more]
gi\|297735275\|emb\|CBI17637.3\|	8.3e-46	50.22	unnamed protein product [Vitis vinifera]	[more]
gi\|225430975\|ref\|XP_002272148.1\|	8.3e-46	50.22	PREDICTED: uncharacterized protein LOC100263320 [Vitis vinifera]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0019915	lipid storage

Vocabulary: **INTERPRO**
Term	Definition
IPR009617	Seipin

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019915	lipid storage
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi06G006960.1	Lsi06G006960.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009617	Seipin family	PFAM	PF06775	Seipin	coord: 12..130 score: 4.2
None	No IPR available	PANTHER	PTHR21212	BERNARDINELLI-SEIP CONGENITAL LIPODYSTROPHY 2 HOMOLOG BSCL2 PROTEIN	coord: 12..238 score: 2.2
None	No IPR available	PANTHER	PTHR21212:SF0	SEIPIN	coord: 12..238 score: 2.2

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi06G006960	Cucumber (Gy14) v1	cgylsiB573
Lsi06G006960	Cucurbita maxima (Rimu)	cmalsiB073
Lsi06G006960	Cucurbita maxima (Rimu)	cmalsiB128
Lsi06G006960	Cucurbita moschata (Rifu)	cmolsiB059
Lsi06G006960	Cucurbita moschata (Rifu)	cmolsiB118
Lsi06G006960	Cucurbita pepo (Zucchini)	cpelsiB017
Lsi06G006960	Cucurbita pepo (Zucchini)	cpelsiB299
Lsi06G006960	Wild cucumber (PI 183967)	cpilsiB059
Lsi06G006960	Cucumber (Chinese Long) v2	culsiB058
Lsi06G006960	Melon (DHL92) v3.5.1	lsimeB398
Lsi06G006960	Watermelon (Charleston Gray)	lsiwcgB361
Lsi06G006960	Watermelon (97103) v1	lsiwmB391
Lsi06G006960	Cucumber (Gy14) v2	cgyblsiB055
Lsi06G006960	Melon (DHL92) v3.6.1	lsimedB420
Lsi06G006960	Silver-seed gourd	carlsiB485
Lsi06G006960	Silver-seed gourd	carlsiB886
Lsi06G006960	Cucumber (Chinese Long) v3	cuclsiB068
Lsi06G006960	Watermelon (97103) v2	lsiwmbB362
Lsi06G006960	Wax gourd	lsiwgoB511
Lsi06G006960	Wax gourd	lsiwgoB521