Lsi06G006950 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi06G006950
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Putative adipose-regulatory protein (Seipin)
Location	chr06 : 11055275 .. 11055892 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTCCGACAACGAACTAGAAGAACCCAACCGCCAAATCCAAAAACCCTCCGATTTCTTCAACAAAATCGTATTCCTCCAAGCCGATTTGATCTACAACGCTATCACCTTTCTAATCGCTCCAGCCTCCACTCTCCTCTCCCTAATCACAGAGTCATTTCACCGAGTAGAAGAGACGAAATGCACCGTCGAGTCCGCCGTCCGAAAATCCCCCTCCGCCATGGCGCTTCAGGTGAGAGTAGCGGTTAGAAGGCTGAGCTACGGGGTTGTGGCGGCGGCGTTGATGTGTATGGTGATGGTGTTGCTTCTAGTAGTTGCGGCGGCGGTGAGTGGGTTGGCGATTAGGTATTGGATGGAAGAGCCGGTGGTGTTGAGGGAGAAATTGAACTTCGATTATACTCAGGCTCGACCTAGGGCTTTGTTTGGAATGGAAAATGGGATGAAGAAGAGGAGGAATTTGGGGATTCCGGTAGGGCACACGTTTTTTGTGTGTGCGGTCCTTTTAATGCCTGAATCTCAATTCAATCGGGAGTTTGGGGTTTTTCAGGTAATGTACGAAATTTATTATTATTATTATTATTTTGCCCCACTAAAAACTAGCTTTATTTATAAATAG

mRNA sequence

ATGGATTCCGACAACGAACTAGAAGAACCCAACCGCCAAATCCAAAAACCCTCCGATTTCTTCAACAAAATCGTATTCCTCCAAGCCGATTTGATCTACAACGCTATCACCTTTCTAATCGCTCCAGCCTCCACTCTCCTCTCCCTAATCACAGAGTCATTTCACCGAGTAGAAGAGACGAAATGCACCGTCGAGTCCGCCGTCCGAAAATCCCCCTCCGCCATGGCGCTTCAGGTGAGAGTAGCGGTTAGAAGGCTGAGCTACGGGGTTGTGGCGGCGGCGTTGATGTGTATGGTGATGGTGTTGCTTCTAGTAGTTGCGGCGGCGGTGAGTGGGTTGGCGATTAGGTATTGGATGGAAGAGCCGGTGGTGTTGAGGGAGAAATTGAACTTCGATTATACTCAGGCTCGACCTAGGGCTTTGTTTGGAATGGAAAATGGGATGAAGAAGAGGAGGAATTTGGGGATTCCGGTAGGGCACACGTTTTTTGTGTGTGCGGTCCTTTTAATGCCTGAATCTCAATTCAATCGGGAGTTTGGGGTTTTTCAGGTAATGTACGAAATTTATTATTATTATTATTATTTTGCCCCACTAAAAACTAGCTTTATTTATAAATAG

Coding sequence (CDS)

ATGGATTCCGACAACGAACTAGAAGAACCCAACCGCCAAATCCAAAAACCCTCCGATTTCTTCAACAAAATCGTATTCCTCCAAGCCGATTTGATCTACAACGCTATCACCTTTCTAATCGCTCCAGCCTCCACTCTCCTCTCCCTAATCACAGAGTCATTTCACCGAGTAGAAGAGACGAAATGCACCGTCGAGTCCGCCGTCCGAAAATCCCCCTCCGCCATGGCGCTTCAGGTGAGAGTAGCGGTTAGAAGGCTGAGCTACGGGGTTGTGGCGGCGGCGTTGATGTGTATGGTGATGGTGTTGCTTCTAGTAGTTGCGGCGGCGGTGAGTGGGTTGGCGATTAGGTATTGGATGGAAGAGCCGGTGGTGTTGAGGGAGAAATTGAACTTCGATTATACTCAGGCTCGACCTAGGGCTTTGTTTGGAATGGAAAATGGGATGAAGAAGAGGAGGAATTTGGGGATTCCGGTAGGGCACACGTTTTTTGTGTGTGCGGTCCTTTTAATGCCTGAATCTCAATTCAATCGGGAGTTTGGGGTTTTTCAGGTAATGTACGAAATTTATTATTATTATTATTATTTTGCCCCACTAAAAACTAGCTTTATTTATAAATAG

Protein sequence

MDSDNELEEPNRQIQKPSDFFNKIVFLQADLIYNAITFLIAPASTLLSLITESFHRVEETKCTVESAVRKSPSAMALQVRVAVRRLSYGVVAAALMCMVMVLLLVVAAAVSGLAIRYWMEEPVVLREKLNFDYTQARPRALFGMENGMKKRRNLGIPVGHTFFVCAVLLMPESQFNREFGVFQVMYEIYYYYYYFAPLKTSFIYK

Homology

[Close]

BLAST of Lsi06G006950 vs. Swiss-Prot
Match: SEI1_ARATH (Seipin-1 OS=Arabidopsis thaliana GN=SEI1 PE=2 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 6.7e-25
Identity = 72/187 (38.50%), Postives = 112/187 (59.89%), Query Frame = 1


Query: 2   DSDNELEEPNRQIQKPSDFFNKIVFLQADLIYNAITFLIAPASTLLSLITESFHRVEETK 61
           + DN+L+ P       +D+F  +V +QADLIYNA+  L +P      L+  S+ R   T 
Sbjct: 11  EQDNQLKIPE---PLRADWFMVLVTIQADLIYNALVVLSSP----FFLLYRSYRRAVVTV 70

Query: 62  CTVESAVRKSPSAMALQVRVAVRRLSYGVVAAALMCMVMVLLLVVAAAVSGLAIRYWMEE 121
              E AV+++P+ +A      VRR  +G++ A  + MVMVL L++A  +    +  ++E+
Sbjct: 71  SAAEKAVKRAPAQIAGGAGRVVRRTWFGILGACHVSMVMVLALILAVVIGVGIVSLYVEK 130

Query: 122 PVVLREKLNFDYTQARPRALFGMENGMKKRRNLGIPVGHTFFVCAVLLMPESQFNREFGV 181
           PVV+R++L FDYT+  P A+F  +   KK+R+  +PVGH+  V  VL MPES+ NR  GV
Sbjct: 131 PVVVRDRLFFDYTEENPSAVFSFD---KKKRSFSVPVGHSVHVSLVLWMPESEINRRIGV 187

Query: 182 FQVMYEI 189
           FQ+  E+
Sbjct: 191 FQLKVEL 187

[Close]

BLAST of Lsi06G006950 vs. Swiss-Prot
Match: SEI3_ARATH (Seipin-3 OS=Arabidopsis thaliana GN=SEI3 PE=2 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 1.6e-07
Identity = 39/122 (31.97%), Postives = 62/122 (50.82%), Query Frame = 1


Query: 83  VRRLSYGVVAAALMCMVMVLLLVVAAAVSGLAIRYWMEEPVVLREKLNFDYTQARPRA-- 142
           VRR+++G+  A  + +++  LLV A  +SG  I Y   EP+V++E LNFDYT++ P A  
Sbjct: 231 VRRVTWGLFCAVYVGIMLFALLVSAFMISGFVITYLAHEPLVIKESLNFDYTKSSPEAYV 290

Query: 143 --------LFGME----------NGMKKRRNLGIPVGHTFFVCAVLLMPESQFNREFGVF 185
                    FG+            G+K R  + +           + +PES++NR  G+F
Sbjct: 291 PISSCAGVAFGLSGKESIETGKVKGLKDRTEITVS----------MTLPESEYNRNLGMF 342

[Close]

BLAST of Lsi06G006950 vs. TrEMBL
Match: A0A0A0M367_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G657470 PE=4 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 1.5e-68
Identity = 146/193 (75.65%), Postives = 165/193 (85.49%), Query Frame = 1


Query: 1   MDSDNELEEPNRQI--QKPSDFFNKIVFLQADLIYNAITFLIAPASTLLSLITESFHRVE 60
           MDSD ELEEPNRQI  +KP+DFFNK+VFLQADLIYNA+ F+IAPA TLLSL  ESF R E
Sbjct: 1   MDSDYELEEPNRQIQIRKPTDFFNKLVFLQADLIYNAMEFVIAPAYTLLSLFAESFQRAE 60

Query: 61  ETKCTVESAVRKSPSAMALQVRVAVRRLSYGVVAAALMCMVMVLLLVVAAAVSGLAIRYW 120
           ETK TVESAVRKSPS +A +V+VA RR+SYG VAA +MCMVMVLLLVVA  VSGL IRYW
Sbjct: 61  ETKHTVESAVRKSPSVVAQRVKVAARRMSYGAVAAGMMCMVMVLLLVVAMGVSGLGIRYW 120

Query: 121 MEEPVVLREKLNFDYTQARPRALFGMENG--MK-KRRNLGIPVGHTFFVCAVLLMPESQF 180
           +EEPV ++EKL FDYT+ARPRALFG  NG  MK K++NLGIPVGHTF+VC VLLMPESQF
Sbjct: 121 IEEPVDVKEKLKFDYTEARPRALFGTGNGNTMKMKKKNLGIPVGHTFYVCVVLLMPESQF 180

Query: 181 NREFGVFQVMYEI 189
           NREFGVFQ+  E+
Sbjct: 181 NREFGVFQLSAEL 193

[Close]

BLAST of Lsi06G006950 vs. TrEMBL
Match: W9S714_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_015131 PE=4 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 2.1e-41
Identity = 93/183 (50.82%), Postives = 131/183 (71.58%), Query Frame = 1


Query: 6   ELEEPNRQIQKPSDFFNKIVFLQADLIYNAITFLIAPASTLLSLITESFHRVEETKCTVE 65
           E E     +   SD+F K+V LQADLIYN+++FL +P   L+S+++ES+HR EET  TVE
Sbjct: 3   EEEVETYDVMPKSDWFTKLVLLQADLIYNSLSFLFSPIFFLMSVVSESYHRAEETTVTVE 62

Query: 66  SAVRKSPSAMALQVRVAVRRLSYGVVAAALMCMVMVLLLVVAAAVSGLAIRYWMEEPVVL 125
           SAV+K PS +     V +++L +GV  AA +CMV++++LVVA  +    ++ W+EEPV+L
Sbjct: 63  SAVKKVPSTVTHGSIVLLKKLGFGVFGAAYVCMVLLVVLVVAVVLGVALVQMWVEEPVLL 122

Query: 126 REKLNFDYTQARPRALFGMENGMKKRRNLGIPVGHTFFVCAVLLMPESQFNREFGVFQVM 185
           +EKL+FDYT+A P+ALF  E    KR+ +G+PVGHTF V  VLLMPES FNR+ GVFQ+ 
Sbjct: 123 KEKLHFDYTEAHPKALFVFERS-HKRKQIGVPVGHTFSVSVVLLMPESDFNRDIGVFQLS 182

Query: 186 YEI 189
            E+
Sbjct: 183 AEL 184

[Close]

BLAST of Lsi06G006950 vs. TrEMBL
Match: A0A0B0N0V2_GOSAR (Seipin OS=Gossypium arboreum GN=F383_30474 PE=4 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 5.0e-35
Identity = 90/196 (45.92%), Postives = 126/196 (64.29%), Query Frame = 1


Query: 1   MDSDNELEEPNRQI---QKPSDFFNKIVFLQADLIYNAITFLIAPASTLLSLITESFHRV 60
           M S  E+EE   ++    KP+D+FN ++ LQADL+YN  T L +P  TLLS   ES+ R 
Sbjct: 1   MLSSPEMEEKEAEVVEAPKPADWFNTLLSLQADLLYNCFTTLSSPFFTLLSAAFESYRRA 60

Query: 61  EETKCTVESAVRKSPSAMALQVRVAVRRLSYGVVAAALMCMVMVLLLVVAAAVSGLAIRY 120
           EET   VE+AV+  PS++     V ++R+  G+  AA MCMV+V L+V+AA V    ++ 
Sbjct: 61  EETTANVETAVQNLPSSITHGSTVLLKRVGLGLFGAAQMCMVLVFLMVLAAFVGIGLVQL 120

Query: 121 WMEEPVVLREKLNFDYTQARPRALF-----GMENGMKKRRNLGIPVGHTFFVCAVLLMPE 180
           W EEPV +REKL FDYT+  P A+F     G + G  +++ +G+PVGHT  V  +LLMPE
Sbjct: 121 WAEEPVFVREKLFFDYTEVNPTAVFCAGGGGFDGGCYRKKQMGVPVGHTIHVYLLLLMPE 180

Query: 181 SQFNREFGVFQVMYEI 189
           S FNR+ GVFQ+  E+
Sbjct: 181 SDFNRDIGVFQLNAEL 196

[Close]

BLAST of Lsi06G006950 vs. TrEMBL
Match: A0A0D2RWN5_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_012G028100 PE=4 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 6.5e-35
Identity = 91/196 (46.43%), Postives = 125/196 (63.78%), Query Frame = 1


Query: 1   MDSDNELEEPNRQI---QKPSDFFNKIVFLQADLIYNAITFLIAPASTLLSLITESFHRV 60
           M S  E+EE   ++    KP+D+FN +V LQADL+YN  T L +P  TLL    ES+ R 
Sbjct: 1   MLSSPEMEEKEAEVVEAPKPADWFNTLVSLQADLLYNCFTTLSSPFFTLLWAAFESYRRA 60

Query: 61  EETKCTVESAVRKSPSAMALQVRVAVRRLSYGVVAAALMCMVMVLLLVVAAAVSGLAIRY 120
           EET   VE+ V+K PS++     V V+R+  G+V AA MCMV+V L+V+AA V    ++ 
Sbjct: 61  EETTANVETVVQKLPSSITHGSTVLVKRVGLGLVGAAQMCMVLVFLMVLAAFVGIGLVQL 120

Query: 121 WMEEPVVLREKLNFDYTQARPRALF-----GMENGMKKRRNLGIPVGHTFFVCAVLLMPE 180
           W EEPV +REKL FDYT+  P A+F     G +    +++ +G+PVGHT  V  +LLMPE
Sbjct: 121 WAEEPVFVREKLFFDYTEVNPTAVFCAGGGGFDGSCYRKKQMGVPVGHTIHVYLLLLMPE 180

Query: 181 SQFNREFGVFQVMYEI 189
           S FNRE G+FQ+  E+
Sbjct: 181 SDFNREIGMFQLNAEL 196

[Close]

BLAST of Lsi06G006950 vs. TrEMBL
Match: V4SM84_9ROSI (Uncharacterized protein (Fragment) OS=Citrus clementina GN=CICLE_v10013740mg PE=4 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 6.1e-33
Identity = 83/188 (44.15%), Postives = 126/188 (67.02%), Query Frame = 1


Query: 4   DNELEEPNRQIQKPSDFFNKIVFLQADLIYNAITFLIAPASTLLSLITESFHRVEETKCT 63
           +NE EE    I KPS++F K+V LQAD+IYN I  L +P  +L S+ ++S+ R E+T  +
Sbjct: 21  ENEKEEFLPLILKPSEWFTKLVSLQADMIYNCIVNLSSPICSLFSVASDSYQRAEKTATS 80

Query: 64  VESAVRKSPSAMALQVRVAVRRLSYGVVAAALMCMVMVLLLVVAAAVSGLAIRYWMEEPV 123
           VE AV+K PS +     + ++++ +GV+ A  +CM+M+ LLV+AA +    ++ W+EEPV
Sbjct: 81  VEMAVQKVPSKLTHGSAIMLKKICFGVLGAIQVCMLMMFLLVLAAVLGVGLVQLWLEEPV 140

Query: 124 VLREKLNFDYTQARPRALF---GMENGMKKRRNLGIPVGHTFFVCAVLLMPESQFNREFG 183
            +R++L FDYT A P+A+F   G+    K +   G+PVGHT+ V  +LLMPES +NR+ G
Sbjct: 141 FVRDRLFFDYTDANPQAIFPVAGVRLAGKGKSQNGVPVGHTYQVSLLLLMPESAYNRDIG 200

Query: 184 VFQVMYEI 189
           VFQ+  EI
Sbjct: 201 VFQLSTEI 208

[Close]

BLAST of Lsi06G006950 vs. TAIR10
Match: AT5G16460.1 (AT5G16460.1 Putative adipose-regulatory protein (Seipin))

HSP 1 Score: 115.5 bits (288), Expect = 3.8e-26
Identity = 72/187 (38.50%), Postives = 112/187 (59.89%), Query Frame = 1


Query: 2   DSDNELEEPNRQIQKPSDFFNKIVFLQADLIYNAITFLIAPASTLLSLITESFHRVEETK 61
           + DN+L+ P       +D+F  +V +QADLIYNA+  L +P      L+  S+ R   T 
Sbjct: 11  EQDNQLKIPE---PLRADWFMVLVTIQADLIYNALVVLSSP----FFLLYRSYRRAVVTV 70

Query: 62  CTVESAVRKSPSAMALQVRVAVRRLSYGVVAAALMCMVMVLLLVVAAAVSGLAIRYWMEE 121
              E AV+++P+ +A      VRR  +G++ A  + MVMVL L++A  +    +  ++E+
Sbjct: 71  SAAEKAVKRAPAQIAGGAGRVVRRTWFGILGACHVSMVMVLALILAVVIGVGIVSLYVEK 130

Query: 122 PVVLREKLNFDYTQARPRALFGMENGMKKRRNLGIPVGHTFFVCAVLLMPESQFNREFGV 181
           PVV+R++L FDYT+  P A+F  +   KK+R+  +PVGH+  V  VL MPES+ NR  GV
Sbjct: 131 PVVVRDRLFFDYTEENPSAVFSFD---KKKRSFSVPVGHSVHVSLVLWMPESEINRRIGV 187

Query: 182 FQVMYEI 189
           FQ+  E+
Sbjct: 191 FQLKVEL 187

[Close]

BLAST of Lsi06G006950 vs. TAIR10
Match: AT2G34380.1 (AT2G34380.1 Putative adipose-regulatory protein (Seipin))

HSP 1 Score: 57.8 bits (138), Expect = 9.3e-09
Identity = 39/122 (31.97%), Postives = 62/122 (50.82%), Query Frame = 1


Query: 83  VRRLSYGVVAAALMCMVMVLLLVVAAAVSGLAIRYWMEEPVVLREKLNFDYTQARPRA-- 142
           VRR+++G+  A  + +++  LLV A  +SG  I Y   EP+V++E LNFDYT++ P A  
Sbjct: 231 VRRVTWGLFCAVYVGIMLFALLVSAFMISGFVITYLAHEPLVIKESLNFDYTKSSPEAYV 290

Query: 143 --------LFGME----------NGMKKRRNLGIPVGHTFFVCAVLLMPESQFNREFGVF 185
                    FG+            G+K R  + +           + +PES++NR  G+F
Sbjct: 291 PISSCAGVAFGLSGKESIETGKVKGLKDRTEITVS----------MTLPESEYNRNLGMF 342

[Close]

BLAST of Lsi06G006950 vs. NCBI nr
Match: gi|659085951|ref|XP_008443693.1| (PREDICTED: uncharacterized protein LOC103487228 [Cucumis melo])

HSP 1 Score: 276.2 bits (705), Expect = 4.7e-71
Identity = 149/195 (76.41%), Postives = 169/195 (86.67%), Query Frame = 1


Query: 1   MDSDNELEEPNRQI----QKPSDFFNKIVFLQADLIYNAITFLIAPASTLLSLITESFHR 60
           MDSDN+L+EPNRQI    QKP+DFFNK+VFLQADLIYNA+ F+IAPASTLLSL  ESF R
Sbjct: 1   MDSDNQLQEPNRQIKIQIQKPTDFFNKLVFLQADLIYNAVGFVIAPASTLLSLFAESFQR 60

Query: 61  VEETKCTVESAVRKSPSAMALQVRVAVRRLSYGVVAAALMCMVMVLLLVVAAAVSGLAIR 120
            EETK TVESAVRKSPS +A +V+VAVRR+SYG VAA +MCMVMVLLL+VA  VSGL IR
Sbjct: 61  AEETKHTVESAVRKSPSLVAQRVKVAVRRVSYGAVAAGMMCMVMVLLLIVAVGVSGLGIR 120

Query: 121 YWMEEPVVLREKLNFDYTQARPRALFGMENG--MK-KRRNLGIPVGHTFFVCAVLLMPES 180
           YW+EEPV ++EKL FDYT+ARPRALFGM NG  MK K++NLGIPVGHTFFVC VLLMPES
Sbjct: 121 YWIEEPVDIKEKLRFDYTEARPRALFGMGNGNTMKMKKKNLGIPVGHTFFVCVVLLMPES 180

Query: 181 QFNREFGVFQVMYEI 189
           QFNREFGVFQ+  E+
Sbjct: 181 QFNREFGVFQLSAEL 195

[Close]

BLAST of Lsi06G006950 vs. NCBI nr
Match: gi|449442797|ref|XP_004139167.1| (PREDICTED: uncharacterized protein LOC101206366 [Cucumis sativus])

HSP 1 Score: 267.3 bits (682), Expect = 2.2e-68
Identity = 146/193 (75.65%), Postives = 165/193 (85.49%), Query Frame = 1


Query: 1   MDSDNELEEPNRQI--QKPSDFFNKIVFLQADLIYNAITFLIAPASTLLSLITESFHRVE 60
           MDSD ELEEPNRQI  +KP+DFFNK+VFLQADLIYNA+ F+IAPA TLLSL  ESF R E
Sbjct: 1   MDSDYELEEPNRQIQIRKPTDFFNKLVFLQADLIYNAMEFVIAPAYTLLSLFAESFQRAE 60

Query: 61  ETKCTVESAVRKSPSAMALQVRVAVRRLSYGVVAAALMCMVMVLLLVVAAAVSGLAIRYW 120
           ETK TVESAVRKSPS +A +V+VA RR+SYG VAA +MCMVMVLLLVVA  VSGL IRYW
Sbjct: 61  ETKHTVESAVRKSPSVVAQRVKVAARRMSYGAVAAGMMCMVMVLLLVVAMGVSGLGIRYW 120

Query: 121 MEEPVVLREKLNFDYTQARPRALFGMENG--MK-KRRNLGIPVGHTFFVCAVLLMPESQF 180
           +EEPV ++EKL FDYT+ARPRALFG  NG  MK K++NLGIPVGHTF+VC VLLMPESQF
Sbjct: 121 IEEPVDVKEKLKFDYTEARPRALFGTGNGNTMKMKKKNLGIPVGHTFYVCVVLLMPESQF 180

Query: 181 NREFGVFQVMYEI 189
           NREFGVFQ+  E+
Sbjct: 181 NREFGVFQLSAEL 193

[Close]

BLAST of Lsi06G006950 vs. NCBI nr
Match: gi|703154948|ref|XP_010111076.1| (hypothetical protein L484_015131 [Morus notabilis])

HSP 1 Score: 177.2 bits (448), Expect = 3.0e-41
Identity = 93/183 (50.82%), Postives = 131/183 (71.58%), Query Frame = 1


Query: 6   ELEEPNRQIQKPSDFFNKIVFLQADLIYNAITFLIAPASTLLSLITESFHRVEETKCTVE 65
           E E     +   SD+F K+V LQADLIYN+++FL +P   L+S+++ES+HR EET  TVE
Sbjct: 3   EEEVETYDVMPKSDWFTKLVLLQADLIYNSLSFLFSPIFFLMSVVSESYHRAEETTVTVE 62

Query: 66  SAVRKSPSAMALQVRVAVRRLSYGVVAAALMCMVMVLLLVVAAAVSGLAIRYWMEEPVVL 125
           SAV+K PS +     V +++L +GV  AA +CMV++++LVVA  +    ++ W+EEPV+L
Sbjct: 63  SAVKKVPSTVTHGSIVLLKKLGFGVFGAAYVCMVLLVVLVVAVVLGVALVQMWVEEPVLL 122

Query: 126 REKLNFDYTQARPRALFGMENGMKKRRNLGIPVGHTFFVCAVLLMPESQFNREFGVFQVM 185
           +EKL+FDYT+A P+ALF  E    KR+ +G+PVGHTF V  VLLMPES FNR+ GVFQ+ 
Sbjct: 123 KEKLHFDYTEAHPKALFVFERS-HKRKQIGVPVGHTFSVSVVLLMPESDFNRDIGVFQLS 182

Query: 186 YEI 189
            E+
Sbjct: 183 AEL 184

[Close]

BLAST of Lsi06G006950 vs. NCBI nr
Match: gi|1009118294|ref|XP_015875781.1| (PREDICTED: seipin-1 [Ziziphus jujuba])

HSP 1 Score: 158.7 bits (400), Expect = 1.1e-35
Identity = 85/188 (45.21%), Postives = 126/188 (67.02%), Query Frame = 1


Query: 6   ELEEPNRQIQKPSDFFNKIVFLQADLIYNAITFLIAPASTLLSLITESFHRVEETKCTVE 65
           E EE    I KP+D+F  I+ LQADLIYN + FL +P  +  S+++ES+ R EE   TVE
Sbjct: 3   EDEEAYYLIPKPTDWFTNIISLQADLIYNCMVFLSSPIFSFFSMVSESYRRAEEVTHTVE 62

Query: 66  SAVRKSPSAMALQVRVAVRRLSYGVVAAALMCMVMVLLLVVAAAVSGLAIRYWMEEPVVL 125
           + V+K PS +   + + +R+L +G + AA +CMV++ + ++A  V    ++ W+EEPV +
Sbjct: 63  TTVQKVPSTITNGIILLLRKLGFGFLGAAYVCMVLIAVFIIAVVVGVGLVQLWVEEPVFV 122

Query: 126 REKLNFDYTQARPRALF---GME--NGMKKRRNLGIPVGHTFFVCAVLLMPESQFNREFG 185
           RE+L FDYT+A P+ALF   G E   G ++++ +G+PVG TF+V   LLMPES FNR+ G
Sbjct: 123 RERLYFDYTEAHPKALFSFGGFEGLQGSQRKKQMGVPVGQTFYVSLALLMPESGFNRDIG 182

Query: 186 VFQVMYEI 189
           VFQ+  EI
Sbjct: 183 VFQLSAEI 190

[Close]

BLAST of Lsi06G006950 vs. NCBI nr
Match: gi|470138139|ref|XP_004304816.1| (PREDICTED: uncharacterized protein LOC101307910 [Fragaria vesca subsp. vesca])

HSP 1 Score: 158.7 bits (400), Expect = 1.1e-35
Identity = 95/207 (45.89%), Postives = 129/207 (62.32%), Query Frame = 1


Query: 2   DSDNELE-EPNRQIQKPSDFFNKIVFLQADLIYNAITFLIAPASTLLSLITESFHRVEET 61
           D D E E E    I KPS++F K+V LQADLIYN++  L  P  +L S   ES+H+ E+ 
Sbjct: 9   DDDEEDEFEDEYLILKPSEWFPKLVSLQADLIYNSLMVLFRPIISLFSFAYESYHQAEKA 68

Query: 62  KCTVESAVRKSPSAMALQVRVAVRRLSYGVVAAALMCMVMVLLLVVAAAVSGLAIRYWME 121
           K TVESAV+ +PS +     +  ++L +GV+ AA +CMVM +LL++A  V   A+R+W+E
Sbjct: 69  KVTVESAVQNAPSTITHGAVLLFKKLGFGVLGAAYVCMVMFMLLILATIVGIAAVRHWLE 128

Query: 122 EPVVLREKLNFDYTQARPRALF---------GMENG----------MKKRRNLGIPVGHT 181
           EPVV+RE L+FDYTQA P+A+F         G+  G            KR  +G+PVGHT
Sbjct: 129 EPVVVREMLHFDYTQANPKAVFVFGGSGSLQGLAAGSGINYFKTISRNKRMPVGVPVGHT 188

Query: 182 FFVCAVLLMPESQFNREFGVFQVMYEI 189
           + V   LLMPES FNR  GVFQ+  E+
Sbjct: 189 YTVSLELLMPESDFNRAIGVFQMTAEV 215

The following BLAST results are available for this feature:

BLAST of Lsi06G006950 vs. Swiss-Prot


Match Name	E-value	Identity	Description
SEI1_ARATH	6.7e-25	38.50	Seipin-1 OS=Arabidopsis thaliana GN=SEI1 PE=2 SV=1	[more]
SEI3_ARATH	1.6e-07	31.97	Seipin-3 OS=Arabidopsis thaliana GN=SEI3 PE=2 SV=1	[more]

BLAST of Lsi06G006950 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0M367_CUCSA	1.5e-68	75.65	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G657470 PE=4 SV=1	[more]
W9S714_9ROSA	2.1e-41	50.82	Uncharacterized protein OS=Morus notabilis GN=L484_015131 PE=4 SV=1	[more]
A0A0B0N0V2_GOSAR	5.0e-35	45.92	Seipin OS=Gossypium arboreum GN=F383_30474 PE=4 SV=1	[more]
A0A0D2RWN5_GOSRA	6.5e-35	46.43	Uncharacterized protein OS=Gossypium raimondii GN=B456_012G028100 PE=4 SV=1	[more]
V4SM84_9ROSI	6.1e-33	44.15	Uncharacterized protein (Fragment) OS=Citrus clementina GN=CICLE_v10013740mg PE=...	[more]

BLAST of Lsi06G006950 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G16460.1	3.8e-26	38.50	Putative adipose-regulatory protein (Seipin)	[more]
AT2G34380.1	9.3e-09	31.97	Putative adipose-regulatory protein (Seipin)	[more]

BLAST of Lsi06G006950 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659085951\|ref\|XP_008443693.1\|	4.7e-71	76.41	PREDICTED: uncharacterized protein LOC103487228 [Cucumis melo]	[more]
gi\|449442797\|ref\|XP_004139167.1\|	2.2e-68	75.65	PREDICTED: uncharacterized protein LOC101206366 [Cucumis sativus]	[more]
gi\|703154948\|ref\|XP_010111076.1\|	3.0e-41	50.82	hypothetical protein L484_015131 [Morus notabilis]	[more]
gi\|1009118294\|ref\|XP_015875781.1\|	1.1e-35	45.21	PREDICTED: seipin-1 [Ziziphus jujuba]	[more]
gi\|470138139\|ref\|XP_004304816.1\|	1.1e-35	45.89	PREDICTED: uncharacterized protein LOC101307910 [Fragaria vesca subsp. vesca]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0019915	lipid storage

Vocabulary: **INTERPRO**
Term	Definition
IPR009617	Seipin

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019915	lipid storage
biological_process	GO:0034389	lipid particle organization
biological_process	GO:0010344	seed oilbody biogenesis
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0005667	transcription factor complex
molecular_function	GO:0003674	molecular_function
molecular_function	GO:0043565	sequence-specific DNA binding
molecular_function	GO:0003700	transcription factor activity, sequence-specific DNA binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi06G006950.1	Lsi06G006950.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009617	Seipin family	PFAM	PF06775	Seipin	coord: 102..188 score: 4.2
None	No IPR available	PANTHER	PTHR21212	BERNARDINELLI-SEIP CONGENITAL LIPODYSTROPHY 2 HOMOLOG BSCL2 PROTEIN	coord: 6..189 score: 8.7
None	No IPR available	PANTHER	PTHR21212:SF0	SEIPIN	coord: 6..189 score: 8.7

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi06G006950	Csa1G657470	Cucumber (Chinese Long) v2	culsiB058
Lsi06G006950	CSPI01G32330	Wild cucumber (PI 183967)	cpilsiB059
Lsi06G006950	Cucsa.356710	Cucumber (Gy14) v1	cgylsiB573
Lsi06G006950	CmaCh11G009380	Cucurbita maxima (Rimu)	cmalsiB128
Lsi06G006950	CmoCh11G009590	Cucurbita moschata (Rifu)	cmolsiB118
Lsi06G006950	Cla009553	Watermelon (97103) v1	lsiwmB391
Lsi06G006950	MELO3C010056	Melon (DHL92) v3.5.1	lsimeB398
Lsi06G006950	ClCG03G010670	Watermelon (Charleston Gray)	lsiwcgB361
Lsi06G006950	MELO3C010056.2	Melon (DHL92) v3.6.1	lsimedB420
Lsi06G006950	Cla97C03G061280	Watermelon (97103) v2	lsiwmbB362
Lsi06G006950	CsGy1G031230	Cucumber (Gy14) v2	cgyblsiB055
Lsi06G006950	Carg22726	Silver-seed gourd	carlsiB485
Lsi06G006950	CsaV3_1G044520	Cucumber (Chinese Long) v3	cuclsiB068

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi06G006950	Wax gourd	lsiwgoB511
Lsi06G006950	Wax gourd	lsiwgoB521
Lsi06G006950	Cucurbita maxima (Rimu)	cmalsiB073
Lsi06G006950	Cucurbita moschata (Rifu)	cmolsiB059
Lsi06G006950	Cucurbita pepo (Zucchini)	cpelsiB017
Lsi06G006950	Cucurbita pepo (Zucchini)	cpelsiB299
Lsi06G006950	Silver-seed gourd	carlsiB886