Lsi06G006300 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi06G006300
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionAluminum activated malate transporter family protein
Locationchr06 : 9154608 .. 9159644 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCATTGTTTTTATAACCCCAACAACACAATACACACAAGCCTTAGCTTCACTCTAACACAATGCCAACTGAGAAAAACTCTAATGGCTATGAAGGGTTGCTACCTTTATGCTTGAGGTTGGTGAAGCCCGTATTCGCGAAGCTCTTGGCCATGGCGGTCGAGCTAGCAACGATAACAAAGAAGCTAGCGAAAGACGACTCGAGAAGGGTAGTTCACGCTTTGAAAGTTGGATTAGCTATAACTTTGGTGTCTTTGTTCTACTATTTCAAACCTTTATATGATGGATTTGGAACGTCTACAATGTGGGCTATCATCACGGTTATTGTCGTCTTTGAGTTTTCGGTTGGTAAGTGTAATATCGATAAAAAATTTTAATTTAATCTTTTATTCGTATCCAATTAGTGAAGATGATGATTATGAGACCAAGACATTCCGATTTTAATATTTTTAATAAACTATATCATTAGAACTAAAAACTCAAGTTGATAGGTTATGATAAATTTTATATATATTCTATTTATTTTCTTAACGCTCGTGACATGTTTTGAACAGGAGGTACACTAGGGCGAGGTTTAAATAGAGTTATGGCGACGTTTTTAGCTGGAGCTCTAGGGTTTGGAGCTCATTATTTGGCAAGCTTGGGTGGAGAGATTGGCCGACCAATAATGCTTGGCTTGTTTGTGTTCGTACTAGGTAAATTTCTAGTTGCTCATTCCTCGAATGTTTCTTGTAATGAAAAATGAGTAAAGACATGATATAGAAATTAGAAAATTTATGTCAACTTAAATATGTATGTAATTATTTCTAATTGGAATATTATTAGTATACAAAAACAAACGCTGAATATTTCTCTTTTGGGGATTGATATTTGTCATCAAATAGGAATAATAAACCACGTGTAAACTTAAGTTTTACATGCTTATAAACCACTTGCAGTCCAAAGAGCTGTTCAATAAAAAACTGCTACATGACAGTTTTTGGTCTTTTTTTATATAAAAATAAAATACCTTTTTTAAGGAAATTTTTTACAAATAAAAAAAATGTCAAACTATTTACATAAAATAACAAAAAAAAAAAAAAATACTGATAGACATTGATAGACTTCTATCAACATCTATCAGTGATAGACATCTATCATTTCTATTGCTGATAGATCCTGATAGACTTCTATCAGCGTTTATCACAACTATTTAAAAAATTTGCTATTTTGTGTAAATAGTTTATCTTATTTTTCTGTTTTTAAAACTTTTCTATTTTTTAATTTTTTTTTTCTATCTGTATTAAAAAAAGGAGAGTATGAGATTAGGTCATGGTTTTGTTGAAAATATGTTGAAAATGAACTATTTGTGCTGTCGAGGAGGTTACTTTGGGATACGTCAAAAAGTAAAATAGCTACCTCTGAGGAATATAATAAACACAAATATCTAAGTGCTCACCGACCTAATGAGTAAATTTCTAGTATCACAAGAATATATAAAATTTTTTATACTCTCTGTTTATTGAGAACTACGTCAATCTGCTTTTAAAAATAAAGTAGGACTATTAAAATTAATGTGTGATCTATTAATATTTATGAATATAAATTATATATAGTGTAAAAAAGTGAGTATGATACTTTCGTAGTACTAAAAAATTTTCTCCTACCTAACATGTGTTTTGACCAATGATATACAATCAAATAGTCGTTCCACAAGATTAGCCGAAGTTTACATAATTGATGAGAATATTCCAGGGAGTTTAACAAATTTGGGATTATTTTTCATCTAAGTTGTGCTGCCCAAAAACATTATTTATTTGTTTCATTTTTATGTAAATATTATATCGTAAATTAAACTAATATATTTTTCTTTTAGTACAACAAGTGTCGTATGAGAATTTAAACCTCTAATTTTCAGAAAAAAAGTATATGTCAATTACGATTGAGTTATGCCCACTTTGACATTCTAATGCTTAAACCTAAGGAAAAATAATTTTATAATATACATTTTTAAAATAATAAATTATGCACACGATCGTAACTTAACAATTTTGATTGTTTGTTTTCCTTAATAGTCACGATTTTACTTATTTGTTTTTTTTTTATATCTATGAGTATTGAGTGTCAAAAAAATTTGTAGAATATTAAATCTTAAGTAGGTAGCTACTATGGATTAAACTCATTCCCTCTAAGCTCTTTATTAAGTCCAACTTAGACGGTAACTACATCATGATTTAACTAGATATCACCTAACTTGCTAACTTGAACATATAGTTCAACGGGTTAAGACATGTTATCTCTGATCAGAAGATCAAAAGTTGGAATCCTCACCCGTACGTGTCATCAAATCATTTAAAAAAAAAAAACTCACCCAAGTACCACTTAAAAGAAATCTATTTTTTGAAAAGCCCATTTCATGAGTAACAAAAATTATTTTTGTTTTGTTTTTGAAATGAATGGCAGCTGGAATATCAACATTCACAAGATTCTTCCCAAAGATGAAGGCAAGATACGACTATGGGCTGCTGATATTCATATTGACATTCTGTATGGTTTCAGTATCAGGATACAGAGATGATGAAATTGCAAAGCTTGCATATAGTAGGATTTTAACCATTCTCATTGGTTGCTTTTTCACTCTCTTTGTTTGCATTTTTGTCAAACCTGTTTGGGCTGGCACTGATCTTCACTATTTGCTCATCACCAACATTCAATCCCTTGCCATATTCTTTCAAGGTAAAATCTTATTTAACCCTACCATCTCATAATTTTGTTTGATAACTATTTTGAGTTTTCATTTTAGTTCTTAAAAATTAACATTATAAATACTTTTTTTTTCTTCTATACTTGATTTTATTTTAAAATTTTGGCTACAAATTTAAATGTTCATATAGAGAAGGTGCAAATCATGGTAAAAAAAATGGTAAGAAACAAGGCACGGGATTTTTAAAATTAAAAAGAAAAAAACTAAAGTTCAGTTTGAGAATAATCTAGTTTCTTTATTTGTTTTGTTGTCTACATTTTAGGCAGGTTTTCAAAATTTAGCTATGATTTTGTTTCCATTCATGTTTCTTTTTAATTATTGTAGCTAAAATTTGAAAGTAAAAAATATATAGCTTTTAAAGAGGGGAATTTTTCCATATAGAAAAAAGGTTAAACTATTTACAGAAAATAACAAAAAAAAAAAAAAAAAAAAGAAAAAGTGATAGACATTGATAGATGAAATCAATATCTATTAGTGATAAACTTCTATCATTTCTATCATTCATAGACTTTGATAGACTTTTATTAGCATCTATCACAACTATCTAAAAATTTTGCTATTTTGTGTAAATAGTTTTCTTTATTTTTCTATTTTTAAACATTTCTCTTTTAAAAAGGGAAATTATTATCAACAAAAAATATCAAACTATTTATAAATATAGAACACTGATAGACAGTAAAATTTTTCTATATTTGAAAACAACCATTAATTTTTTTTTTTTGTTTTGTTTTTAGAATTTAGTTAAAAATCCAAATTTAATACATTGGTAAACCATGAGTAAAAAATTGTAATTAAAATGAGACCTAAATAGTTACCGAATTGGACTTAAATTGTTGTCTAAATTTGAAGGTGCGGTTTGATAATAATTAATCTAATTTCTTTTAACTTGGTTAAGGAATGTATATTATGAACCATTGGCAGGGTTTGGGGTTGAATATTTTGGATTATCACAAGAAGAAGAGATTTGCAAGGCTGACATGCAAAAATATAGAACTATTCTGAATTCGAAATCGAGTGAAGAATCATTGGTGAGTACTCGAGCTCGATCCAGCTATAAATCTTTTAGGACTTGTTTGATAATCTTCTCATTCCTGCATATATATATATATATATATATATATATATATATTTGGGCAGAGTAACCTTGCAAGATGGGAGCCTCGGCATGGGAAGTTCAGATATCGGCATCCTTGGAACCAATATCTGGAAATTGGAAGCTTAAATAGGGACTGTGCCTATCGCCTCGAACTTCTTAATGGATACCTCAAACCCAACCAATTTCAAGTAAGATTCAATGCAAATTTTGATCCTCGACTTTTAGAATTGTATCGATTGGATTTCTACGTACTTTAAAAAGTTTATAATAGATCTATAACTAATTTCAATTTTGCATGTAATAAATCTTTTCAACTCCGCTAGTTTATATATGTTATATGCTTACTACTCTATTATTTACATATGCTTACTACTTTATTATTTAATGATTAGTAAAAACTATTTTTTTCTTCTAGTTTTTAAAACTTGTTCGGATAAGGTAGGTAACAAAATAATAAAGAAATTTAGAAGTGAAAGTGAAGATAATTAAGCTTAATTTTCAAAACAACAAATATAAAAAAACCAAATAGCTATCAAACTCAAAGTACTTTATTTTTTATTTATTTATTTTAGAATTTGGTTAAGAATACAAATACATCTTAATAGAGAAGAAAACCATGGCAAATAAACATGGTGAGAAAACAAGTACTAAGTTTTCAAAAACAAAAAACCAAATAATTATAAAAAACAAAAATCTAATGAAAAACCCTATATATGCTGATGATCTTTTCATTTGCAGATGCCATCGCAACAAATCCATGGTCAATTTCAAGAGGAATGCATGAAGATATGCAGTGAATCAAGTAGTGGATTAAGAGAATTAGCATTATCACTAAGAAAAATGGTTCCTCCACTAACAGCCAAATCCCACATAGAGAAAGCAAAGATTGCAGCTGAGAATCTCAAGACCCATTTGGAAGAATGGAGATTTGAAGAAGTGAATAATGCTATGGAGATTGTTCATATTGTTTCTTTGGCATCACTTCTATTTGATACAATTTGCTGCATAGAGAAGATTGTGGATTCAGTTCAAAAGTTGGCTTCAATGGCTGGTTTCAAGGCTGTGGAAATGCAATCAAGTGTAGCACCACAGCAACAAAAAGATTTGCAAGATGAAGATTCATTGCAGCCATGCCATGGAACTACTATCCTTTTAGCTCATCATGTCATTACAGTTGATGAAGGAGTTCCTTGTTAA

mRNA sequence

ATGGCATTGTTGGTGAAGCCCGTATTCGCGAAGCTCTTGGCCATGGCGGTCGAGCTAGCAACGATAACAAAGAAGCTAGCGAAAGACGACTCGAGAAGGGTAGTTCACGCTTTGAAAGTTGGATTAGCTATAACTTTGGTGTCTTTGTTCTACTATTTCAAACCTTTATATGATGGATTTGGAACGTCTACAATGTGGGCTATCATCACGGTTATTGTCGTCTTTGAGTTTTCGGTTGGAGGTACACTAGGGCGAGGTTTAAATAGAGTTATGGCGACGTTTTTAGCTGGAGCTCTAGGGTTTGGAGCTCATTATTTGGCAAGCTTGGGTGGAGAGATTGGCCGACCAATAATGCTTGGCTTGTTTGTGTTCGTACTAGCTGGAATATCAACATTCACAAGATTCTTCCCAAAGATGAAGGCAAGATACGACTATGGGCTGCTGATATTCATATTGACATTCTGTATGGTTTCAGTATCAGGATACAGAGATGATGAAATTGCAAAGCTTGCATATAGTAGGATTTTAACCATTCTCATTGGTTGCTTTTTCACTCTCTTTGTTTGCATTTTTGTCAAACCTGTTTGGGCTGGCACTGATCTTCACTATTTGCTCATCACCAACATTCAATCCCTTGCCATATTCTTTCAAGGGTTTGGGGTTGAATATTTTGGATTATCACAAGAAGAAGAGATTTGCAAGGCTGACATGCAAAAATATAGAACTATTCTGAATTCGAAATCGAGTGAAGAATCATTGAGTAACCTTGCAAGATGGGAGCCTCGGCATGGGAAGTTCAGATATCGGCATCCTTGGAACCAATATCTGGAAATTGGAAGCTTAAATAGGGACTGTGCCTATCGCCTCGAACTTCTTAATGGATACCTCAAACCCAACCAATTTCAAGTAAGATTCAATGCAAATTTTGATCCTCGACTTTTAGAATTGTATCGATTGGATTTCTACATATGCAGTGAATCAAGTAGTGGATTAAGAGAATTAGCATTATCACTAAGAAAAATGGTTCCTCCACTAACAGCCAAATCCCACATAGAGAAAGCAAAGATTGCAGCTGAGAATCTCAAGACCCATTTGGAAGAATGGAGATTTGAAGAAGTGAATAATGCTATGGAGATTGTTCATATTGTTTCTTTGGCATCACTTCTATTTGATACAATTTGCTGCATAGAGAAGATTGTGGATTCAGTTCAAAAGTTGGCTTCAATGGCTGGTTTCAAGGCTGTGGAAATGCAATCAAGTGTAGCACCACAGCAACAAAAAGATTTGCAAGATGAAGATTCATTGCAGCCATGCCATGGAACTACTATCCTTTTAGCTCATCATGTCATTACAGTTGATGAAGGAGTTCCTTGTTAA

Coding sequence (CDS)

ATGGCATTGTTGGTGAAGCCCGTATTCGCGAAGCTCTTGGCCATGGCGGTCGAGCTAGCAACGATAACAAAGAAGCTAGCGAAAGACGACTCGAGAAGGGTAGTTCACGCTTTGAAAGTTGGATTAGCTATAACTTTGGTGTCTTTGTTCTACTATTTCAAACCTTTATATGATGGATTTGGAACGTCTACAATGTGGGCTATCATCACGGTTATTGTCGTCTTTGAGTTTTCGGTTGGAGGTACACTAGGGCGAGGTTTAAATAGAGTTATGGCGACGTTTTTAGCTGGAGCTCTAGGGTTTGGAGCTCATTATTTGGCAAGCTTGGGTGGAGAGATTGGCCGACCAATAATGCTTGGCTTGTTTGTGTTCGTACTAGCTGGAATATCAACATTCACAAGATTCTTCCCAAAGATGAAGGCAAGATACGACTATGGGCTGCTGATATTCATATTGACATTCTGTATGGTTTCAGTATCAGGATACAGAGATGATGAAATTGCAAAGCTTGCATATAGTAGGATTTTAACCATTCTCATTGGTTGCTTTTTCACTCTCTTTGTTTGCATTTTTGTCAAACCTGTTTGGGCTGGCACTGATCTTCACTATTTGCTCATCACCAACATTCAATCCCTTGCCATATTCTTTCAAGGGTTTGGGGTTGAATATTTTGGATTATCACAAGAAGAAGAGATTTGCAAGGCTGACATGCAAAAATATAGAACTATTCTGAATTCGAAATCGAGTGAAGAATCATTGAGTAACCTTGCAAGATGGGAGCCTCGGCATGGGAAGTTCAGATATCGGCATCCTTGGAACCAATATCTGGAAATTGGAAGCTTAAATAGGGACTGTGCCTATCGCCTCGAACTTCTTAATGGATACCTCAAACCCAACCAATTTCAAGTAAGATTCAATGCAAATTTTGATCCTCGACTTTTAGAATTGTATCGATTGGATTTCTACATATGCAGTGAATCAAGTAGTGGATTAAGAGAATTAGCATTATCACTAAGAAAAATGGTTCCTCCACTAACAGCCAAATCCCACATAGAGAAAGCAAAGATTGCAGCTGAGAATCTCAAGACCCATTTGGAAGAATGGAGATTTGAAGAAGTGAATAATGCTATGGAGATTGTTCATATTGTTTCTTTGGCATCACTTCTATTTGATACAATTTGCTGCATAGAGAAGATTGTGGATTCAGTTCAAAAGTTGGCTTCAATGGCTGGTTTCAAGGCTGTGGAAATGCAATCAAGTGTAGCACCACAGCAACAAAAAGATTTGCAAGATGAAGATTCATTGCAGCCATGCCATGGAACTACTATCCTTTTAGCTCATCATGTCATTACAGTTGATGAAGGAGTTCCTTGTTAA

Protein sequence

MALLVKPVFAKLLAMAVELATITKKLAKDDSRRVVHALKVGLAITLVSLFYYFKPLYDGFGTSTMWAIITVIVVFEFSVGGTLGRGLNRVMATFLAGALGFGAHYLASLGGEIGRPIMLGLFVFVLAGISTFTRFFPKMKARYDYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTILIGCFFTLFVCIFVKPVWAGTDLHYLLITNIQSLAIFFQGFGVEYFGLSQEEEICKADMQKYRTILNSKSSEESLSNLARWEPRHGKFRYRHPWNQYLEIGSLNRDCAYRLELLNGYLKPNQFQVRFNANFDPRLLELYRLDFYICSESSSGLRELALSLRKMVPPLTAKSHIEKAKIAAENLKTHLEEWRFEEVNNAMEIVHIVSLASLLFDTICCIEKIVDSVQKLASMAGFKAVEMQSSVAPQQQKDLQDEDSLQPCHGTTILLAHHVITVDEGVPC
BLAST of Lsi06G006300 vs. Swiss-Prot
Match: ALMT2_ARATH (Aluminum-activated malate transporter 2 OS=Arabidopsis thaliana GN=ALMT2 PE=2 SV=2)

HSP 1 Score: 367.9 bits (943), Expect = 1.7e-100
Identity = 198/437 (45.31%), Postives = 290/437 (66.36%), Query Frame = 1

Query: 18  ELATITKKLAKDDSRRVVHALKVGLAITLVSLFYYFKPLYDGFGTSTMWAIITVIVVFEF 77
           E+    +++ K+D RRVVHA KVGLA+ LVS FYY++PLYD FG + MWA++TV+VVFEF
Sbjct: 6   EIVREGRRVGKEDPRRVVHAFKVGLALALVSSFYYYQPLYDNFGVNAMWAVMTVVVVFEF 65

Query: 78  SVGGTLGRGLNRVMATFLAGALGFGAHYLASLGGEIGRPIMLGLFVFVLAGISTFTRFFP 137
           SVG TLG+GLNR +AT +AG LG GAH+LASL G    PI+L +FVFVLA +STF RFFP
Sbjct: 66  SVGATLGKGLNRAVATLVAGGLGIGAHHLASLSGPTVEPILLAIFVFVLAALSTFVRFFP 125

Query: 138 KMKARYDYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTILIGCFFTLFVCIFVKPVWA 197
           ++KARYDYG+LIFILTF ++SVSG+R+DEI  LA+ R+ T+++G    + + IFV PVWA
Sbjct: 126 RVKARYDYGVLIFILTFALISVSGFREDEILDLAHKRLSTVIMGGVSCVLISIFVCPVWA 185

Query: 198 GTDLHYLLITNIQSLAIFFQGFGVEYFGLSQEEEICKAD-----MQKYRTILNSKSSEES 257
           G DLH LL +N  +L+ F Q FG EYF  +++ +I + +     +++Y+++LNSKS+EE+
Sbjct: 186 GQDLHSLLASNFDTLSHFLQEFGDEYFEATEDGDIKEVEKRRRNLERYKSVLNSKSNEEA 245

Query: 258 LSNLARWEPRHGKFRYRHPWNQYLEIGSLNRDCAYRLELLNGYLKPNQFQVRFNANFDPR 317
           L+N A+WEPRHG+FR+RHPW QYL +G+L R  AYR++ LN  +  +   ++   +   +
Sbjct: 246 LANFAKWEPRHGQFRFRHPWRQYLAVGALLRQSAYRIDALNSNINSD---MQIPMDIKKK 305

Query: 318 LLELYRLDFYICSESSSGLRELALSLRKMVPPLTAKSHIEKAKIAAENLKTHLEEWRFEE 377
           + E  R    + SES   ++E+++SL+ M    +   H+  ++ A + L T L+     +
Sbjct: 306 IEEPLR---RMSSESGKSMKEVSISLKNMTISSSFDIHVVNSQSACKTLSTLLKSGILND 365

Query: 378 VNNAMEIVHIVSLASLLFDTICCIEKIVDSVQKLASMAGFKAVEMQS-----SVAPQQQK 437
           V   ++++ +++  SLL D +   EKI +SV +LAS A FK  +  S     S+      
Sbjct: 366 V-EPLQMISLMTTVSLLIDIVNLTEKISESVHELASAAKFKNKKKPSKSNSGSIGQAMPN 425

Query: 438 DLQDEDSLQPCHGTTIL 445
              D+D     H  TIL
Sbjct: 426 KSHDDDD----HVVTIL 431

BLAST of Lsi06G006300 vs. Swiss-Prot
Match: ALMT7_ARATH (Aluminum-activated malate transporter 7 OS=Arabidopsis thaliana GN=ALMT7 PE=3 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 1.1e-99
Identity = 196/428 (45.79%), Postives = 272/428 (63.55%), Query Frame = 1

Query: 18  ELATITKKLAKDDSRRVVHALKVGLAITLVSLFYYFKPLYDGFGTSTMWAIITVIVVFEF 77
           E+    +++AK+D RRVVH+ KVGL + LVS FYY++PLYD FG + MWA++TV+VVFEF
Sbjct: 6   EIVREGRRVAKEDPRRVVHSFKVGLVLALVSSFYYYQPLYDSFGVNAMWAVMTVVVVFEF 65

Query: 78  SVGGTLGRGLNRVMATFLAGALGFGAHYLASLGGEIGRPIMLGLFVFVLAGISTFTRFFP 137
           SVG TLG+GLNRV AT  AG LG GAH+LAS+ G  G PI+L +FVFV A +STF RFFP
Sbjct: 66  SVGATLGKGLNRVAATLFAGGLGIGAHHLASMSGPTGEPILLAVFVFVQAALSTFVRFFP 125

Query: 138 KMKARYDYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTILIGCFFTLFVCIFVKPVWA 197
           ++KARYDY LLIFILTF ++SVSG+R++++ KL + RI T++IG    + + IFV PVWA
Sbjct: 126 RVKARYDYSLLIFILTFALISVSGFREEQVVKLTHKRISTVIIGGLSCVIISIFVCPVWA 185

Query: 198 GTDLHYLLITNIQSLAIFFQG--------------------------FGVEYFGL----- 257
           G DLH L+ +N + L+ F  G                          FG +Y  +     
Sbjct: 186 GQDLHSLIASNFEKLSFFLLGNSFHYVSSDLNSITLLRKIKSWRLADFGDKYCEVVENDG 245

Query: 258 SQEEEICKADMQKYRTILNSKSSEESLSNLARWEPRHGKFRYRHPWNQYLEIGSLNRDCA 317
           ++E +  K D   Y+++LNSKS+EESL+N A+WEP HG+FR+RHPW QYL +G L R CA
Sbjct: 246 AKEVDKRKKDFDNYKSVLNSKSNEESLANFAKWEPGHGQFRFRHPWKQYLAVGELIRQCA 305

Query: 318 YRLELLNGYLK-PNQFQVRFNANFDPRLLELYRLDFYICSESSSGLRELALSLRKMVPPL 377
           YR+  LN YL   NQ  V         L  +         ES   ++E+++SL+KM  P 
Sbjct: 306 YRIHALNSYLNADNQVSVDIKKKLGEPLRRM-------SLESGKAMKEMSISLKKMTKPS 365

Query: 378 TAKSHIEKAKIAAENLKTHLEEWRFEEVNNAMEIVHIVSLASLLFDTICCIEKIVDSVQK 414
           ++  H++ AK A ++L   L     +EV   +E+V +++  SLL D I   EKI++S+ +
Sbjct: 366 SSDLHVQNAKSACKSLTNLLNSGILKEV-EPLELVSLLTAISLLIDIINLTEKILESLHE 425

BLAST of Lsi06G006300 vs. Swiss-Prot
Match: ALMT8_ARATH (Aluminum-activated malate transporter 8 OS=Arabidopsis thaliana GN=ALMT8 PE=3 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 3.2e-96
Identity = 187/391 (47.83%), Postives = 263/391 (67.26%), Query Frame = 1

Query: 24  KKLAKDDSRRVVHALKVGLAITLVSLFYYFKPLYDGFGTSTMWAIITVIVVFEFSVGGTL 83
           +K AKDD RR++H++KVG+A+TLVSL YY +PLY  FG + MWAI+TV+VVFEF+VGGTL
Sbjct: 35  QKFAKDDPRRIIHSMKVGVALTLVSLLYYVRPLYISFGVTGMWAILTVVVVFEFTVGGTL 94

Query: 84  GRGLNRVMATFLAGALGFGAHYLASLGGEIGRPIMLGLFVFVLAGISTFTRFFPKMKARY 143
            +GLNR  AT +AGALG GA +LA   G  G PI+LG+ VF L   +TF+RFFP++K RY
Sbjct: 95  SKGLNRGFATLIAGALGVGAVHLARFFGHQGEPIVLGILVFSLGAAATFSRFFPRIKQRY 154

Query: 144 DYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTILIGCFFTLFVCIFVKPVWAGTDLHY 203
           DYG LIFILTF  V++SGYR DEI  +AY R+ TILIG    + V IF+ PVWAG DLH 
Sbjct: 155 DYGALIFILTFSFVAISGYRTDEILIMAYQRLSTILIGGTICILVSIFICPVWAGEDLHK 214

Query: 204 LLITNIQSLAIFFQGFGVEYFGLSQEEEICKADMQKYRTILNSKSSEESLSNLARWEPRH 263
           ++  NI  LA + +GF  EYF   +  +   + +++Y++IL SKS+E+SL+NLARWEP H
Sbjct: 215 MIANNINKLAKYLEGFEGEYFQPEKISKETSSCVREYKSILTSKSTEDSLANLARWEPGH 274

Query: 264 GKFRYRHPWNQYLEIGSLNRDCAYRLELLNGYLKPNQFQVRFNANFDPRLLELYRLDFYI 323
           G+FR RHPW +YL+I  L R CA  LE+LNGY+  N    +    F+ ++ E       +
Sbjct: 275 GRFRLRHPWKKYLKIAGLVRQCAVHLEILNGYVLSND---KAPQEFESKIQEPITT---M 334

Query: 324 CSESSSGLRELALSLRKM-VPPLTAKSHIEKAKIAAENLKTHLEEWRFEEVNNAMEIVHI 383
             E    L+ +A S++ M        +HI+ +K A +NLK  L+    E   + +EI+  
Sbjct: 335 SREVGEALKAIAKSIKTMRNDSACVNAHIDNSKKAIKNLKIALKSSYPETYKDLLEIIPG 394

Query: 384 VSLASLLFDTICCIEKIVDSVQKLASMAGFK 414
           V++AS+L + + C+EKI ++V++ + +A FK
Sbjct: 395 VTMASILIEVVNCVEKIYEAVEEFSGLAHFK 419

BLAST of Lsi06G006300 vs. Swiss-Prot
Match: ALMT1_ARATH (Aluminum-activated malate transporter 1 OS=Arabidopsis thaliana GN=ALMT1 PE=1 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 1.5e-93
Identity = 188/437 (43.02%), Postives = 279/437 (63.84%), Query Frame = 1

Query: 25  KLAKDDSRRVVHALKVGLAITLVSLFYYFKPL---YDGFGTSTMWAIITVIVVFEFSVGG 84
           ++  +D RR++HA KVGLA+ LVS FYY++P     D FG + MWA++TV+VVFEFSVG 
Sbjct: 13  RVGNEDPRRIIHAFKVGLALVLVSSFYYYQPFGPFTDYFGINAMWAVMTVVVVFEFSVGA 72

Query: 85  TLGRGLNRVMATFLAGALGFGAHYLASLGGEIGRPIMLGLFVFVLAGISTFTRFFPKMKA 144
           TLG+GLNR +AT +AG LG GAH LA L G    PI+L + VFV A +STF RFFP +K 
Sbjct: 73  TLGKGLNRGVATLVAGGLGIGAHQLARLSGATVEPILLVMLVFVQAALSTFVRFFPWVKT 132

Query: 145 RYDYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTILIGCFFTLFVCIFVKPVWAGTDL 204
           ++DYG+LIFILTF ++S+SG+RD+EI  LA SR+ T++IG    + + IFV PVWAG DL
Sbjct: 133 KFDYGILIFILTFALISLSGFRDEEIMDLAESRLSTVVIGGVSCILISIFVCPVWAGQDL 192

Query: 205 HYLLITNIQSLAIFFQGFGVEYF-----GLSQEEEICKADMQKYRTILNSKSSEESLSNL 264
           H LL +N  +L+ F Q FG EYF     G  +  E  K ++++Y+++L+SKS EE+L+N 
Sbjct: 193 HSLLASNFDTLSHFLQDFGDEYFEAREKGDYKVVEKRKKNLERYKSVLDSKSDEEALANY 252

Query: 265 ARWEPRHGKFRYRHPWNQYLEIGSLNRDCAYRLELLNGYLKPN-QFQVRFNANFDPRLLE 324
           A WEP HG+FR+RHPW QY+ +G+L R CAYR++ LN Y+  + Q  V      +  L  
Sbjct: 253 AEWEPPHGQFRFRHPWKQYVAVGALLRQCAYRIDALNSYINSDFQIPVDIKKKLETPLRR 312

Query: 325 LYRLDFYICSESSSGLRELALSLRKMVPPLTAKSHIEKAKIAAENLKTHLEEWRFEEVNN 384
           +        SES + ++E+++SL++M+   ++  H+  ++ A ++L T L+     +V  
Sbjct: 313 M-------SSESGNSMKEMSISLKQMIKSSSSDIHVSNSQAACKSLSTLLKSGILNDV-E 372

Query: 385 AMEIVHIVSLASLLFDTICCIEKIVDSVQKLASMAGFKAVEMQSSVAPQQQKDLQDEDSL 444
            ++++ +++  S+L D +   EKI +SV +LAS A FK     + + P    +  D  S+
Sbjct: 373 PLQMISLMTTVSMLIDIVNLTEKISESVHELASAARFK-----NKMRPTVLYEKSDSGSI 432

Query: 445 QPCHGTTILLAHHVITV 453
                      HHV+TV
Sbjct: 433 GRAMPIDSHEDHHVVTV 436

BLAST of Lsi06G006300 vs. Swiss-Prot
Match: ALMT1_WHEAT (Aluminum-activated malate transporter 1 OS=Triticum aestivum GN=ALMT1 PE=1 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 4.0e-78
Identity = 175/429 (40.79%), Postives = 260/429 (60.61%), Query Frame = 1

Query: 3   LLVKPVFAKLLAMAVELATITKKLAKDDSRRVVHALKVGLAITLVSLFYYFKPLYDGFGT 62
           LL+  + A L   A   A      A++D RRV H+LKVGLA+ LVS+ Y+  PL++G G 
Sbjct: 22  LLLHSLLAGLGRRAAGFARKVGGAAREDPRRVAHSLKVGLALALVSVVYFVTPLFNGLGV 81

Query: 63  STMWAIITVIVVFEFSVGGTLGRGLNRVMATFLAGALGFGAHYLASLG---GEIGRPIML 122
           S +WA++TV+VV E++VG TL +GLNR +AT +AG +  GAH LA L    G+ G PI+L
Sbjct: 82  SAIWAVLTVVVVMEYTVGATLSKGLNRALATLVAGCIAVGAHQLAELAERCGDQGEPIVL 141

Query: 123 GLFVFVLAGISTFTRFFPKMKARYDYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTIL 182
            + VF +A  +TF RF P++KA+YDYG+ IFILTF +V+VS YR +E+ +LA+ R  TI 
Sbjct: 142 TVLVFFVASAATFLRFIPEIKAKYDYGVTIFILTFGLVAVSSYRVEELIQLAHQRFYTIA 201

Query: 183 IGCFFTLFVCIFVKPVWAGTDLHYLLITNIQSLAIFFQGFGVEYFGLSQEEEIC-----K 242
           +G F  L   +F+ PVWAG D+H L   N+  LA F +G     FG   E  +      K
Sbjct: 202 VGVFICLCTTVFLFPVWAGEDVHKLASGNLDKLAQFIEGMEFNCFG---ENSVANNFGGK 261

Query: 243 ADMQKYRTILNSKSSEESLSNLARWEPRHGKFRYRHPWNQYLEIGSLNRDCAYRLELLNG 302
              Q ++++LNSK++E+SL   A+WEPRHG+FR+RHPW+QY ++G+L R CA  +E L  
Sbjct: 262 DSPQMHKSVLNSKATEDSLCTFAKWEPRHGQFRFRHPWSQYQKLGTLCRQCASSMEALAS 321

Query: 303 YLKPNQFQVRFNANFDPRL-LELYRLDFYICSESSSGLRELALSLRKMVPPLTAKSHIEK 362
           Y+     + +  A  +P L  ++ +    +   SS  LR+LA++ R M  P      +  
Sbjct: 322 YVITTS-KTQCPAAANPELSCKVRKTCGEMSLHSSKVLRDLAMATRTMTVPSPVNITMAT 381

Query: 363 AKIAAENLKTHLEEWRFEEVNNA-MEIVHIVSLASLLFDTICCIEKIVDSVQKLASMAGF 422
           A  AAE+L++ L E      N A ++++H+   A+LL D +  +++I + V  LA +A F
Sbjct: 382 AVKAAESLRSELAE------NTALLQVMHVAVTATLLADLVDRVKEIAECVDVLARLAHF 440

BLAST of Lsi06G006300 vs. TrEMBL
Match: A0A0A0M301_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G630850 PE=4 SV=1)

HSP 1 Score: 710.3 bits (1832), Expect = 1.5e-201
Identity = 366/460 (79.57%), Postives = 405/460 (88.04%), Query Frame = 1

Query: 5   VKPVFAKLLAMAVELATITKKLAKDDSRRVVHALKVGLAITLVSLFYYFKPLYDGFGTST 64
           +KP+FAKL  ++V+LAT+ KKLAKDDSRRVVHALKVGLAI+LVSL YYFKPLYDGFGTST
Sbjct: 21  LKPIFAKLSTVSVKLATMAKKLAKDDSRRVVHALKVGLAISLVSLLYYFKPLYDGFGTST 80

Query: 65  MWAIITVIVVFEFSVGGTLGRGLNRVMATFLAGALGFGAHYLASLGGEIGRPIMLGLFVF 124
           MWAI+TVIVVFEFSVGGTLGRGLNRVMAT LAG LGFG HYLASLGG+ GRPI+L LFVF
Sbjct: 81  MWAIVTVIVVFEFSVGGTLGRGLNRVMATLLAGGLGFGTHYLASLGGDTGRPIILALFVF 140

Query: 125 VLAGISTFTRFFPKMKARYDYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTILIGCFF 184
           +LA +STFTRFFPK+KARYDYGLLI ILTFCMVS+SGYRD+EIAKLA SRILTILIGC  
Sbjct: 141 ILASVSTFTRFFPKIKARYDYGLLILILTFCMVSLSGYRDEEIAKLALSRILTILIGCCV 200

Query: 185 TLFVCIFVKPVWAGTDLHYLLITNIQSLAIFFQGFGVEYFGLSQEEEICKADMQKYRTIL 244
           TL VCIFV+PVWAGTDLH L+  NIQSLA+FFQGFG E+FGLSQE E+   DMQKYRTIL
Sbjct: 201 TLIVCIFVRPVWAGTDLHCLVANNIQSLALFFQGFGAEFFGLSQEGEVSNDDMQKYRTIL 260

Query: 245 NSKSSEESLSNLARWEPRHGKFRYRHPWNQYLEIGSLNRDCAYRLELLNGYLKPNQFQV- 304
           NSKS+EESL+NLARWEPRHGKFRYRHPW QYL+IGSLNR+CAYRLELLNGYLK NQFQ+ 
Sbjct: 261 NSKSNEESLTNLARWEPRHGKFRYRHPWKQYLKIGSLNRECAYRLELLNGYLKTNQFQMP 320

Query: 305 --RFNANFDPRLLELYRLDFYICSESSSGLRELALSLRKMVPPLTAKSHIEKAKIAAENL 364
             + +  F    ++       ICSESS GLRELAL+LRKMV PLTAKSHIEKAKIAAENL
Sbjct: 321 SQQIHGQFKEECMK-------ICSESSRGLRELALALRKMVLPLTAKSHIEKAKIAAENL 380

Query: 365 KTHLEEWRFEEVNNAMEIVHIVSLASLLFDTICCIEKIVDSVQKLASMAGFKAVEMQSSV 424
           K+HLEEWRFEEVNNAMEIV +VSLASLLFDTICCIEKIVDSVQ+LASMAGFKAVE+QSSV
Sbjct: 381 KSHLEEWRFEEVNNAMEIVQVVSLASLLFDTICCIEKIVDSVQELASMAGFKAVEVQSSV 440

Query: 425 APQQQKDLQDED--SLQP-CHGTTILLAHHVITVDEGVPC 459
           AP+QQ DLQD+D  +LQP  HG  +LLAHH IT+DE  PC
Sbjct: 441 APEQQMDLQDQDQYALQPLSHGAAVLLAHHAITIDEQSPC 473

BLAST of Lsi06G006300 vs. TrEMBL
Match: B9HQ62_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0009s02310g PE=4 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 2.2e-123
Identity = 245/455 (53.85%), Postives = 316/455 (69.45%), Query Frame = 1

Query: 5   VKPVFAKLLAMAVELATITKKLAKDDSRRVVHALKVGLAITLVSLFYYFKPLYDGFGTST 64
           +K +  +  A  VE A   KKL +DD RRV H++KVGLAITLVSLFYYF+PLYDGFG S 
Sbjct: 15  IKSLPERSKAKIVECARKIKKLGQDDPRRVNHSVKVGLAITLVSLFYYFEPLYDGFGDSA 74

Query: 65  MWAIITVIVVFEFSVGGTLGRGLNRVMATFLAGALGFGAHYLASLGGEIGRPIMLGLFVF 124
           MWA++TV+VVFEFSVG TLGRGLNR +ATFLAGALGFGAH LA+L GE G P++LGLFVF
Sbjct: 75  MWAVMTVVVVFEFSVGATLGRGLNRGLATFLAGALGFGAHRLATLSGEKGEPMLLGLFVF 134

Query: 125 VLAGISTFTRFFPKMKARYDYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTILIGCFF 184
           +LA   TF RFFP+MKARYDYGLLIFILTFC++SVSGYRDDE+  +A+ R+ TILIG   
Sbjct: 135 LLATTVTFVRFFPRMKARYDYGLLIFILTFCLISVSGYRDDEMLDMAHKRVSTILIGSLT 194

Query: 185 TLFVCIFVKPVWAGTDLHYLLITNIQSLAIFFQGFGVEYFGLSQE-EEICKADMQKYRTI 244
            +FVCI + PVWAG DLH L  TNI+ L IF + FGVE+F    E E I KA +Q Y+++
Sbjct: 195 AVFVCICICPVWAGDDLHNLAATNIEKLGIFLEHFGVEFFRKPGEGESINKASLQGYKSV 254

Query: 245 LNSKSSEESLSNLARWEPRHGKFRYRHPWNQYLEIGSLNRDCAYRLELLNGYLKPNQFQV 304
           LNSK+ EESL N ARWEP HG+F++RHPW  YL+ GSL R CAYR+E LNGYL       
Sbjct: 255 LNSKNMEESLVNFARWEPGHGQFKFRHPWKHYLKFGSLTRQCAYRVEALNGYLNS----- 314

Query: 305 RFNANFDPRLLELYRLD-FYICSESSSGLRELALSLRKMVPPLTAKSHIEKAKIAAENLK 364
             +    P +  + +     + SE    L+ELAL++++M PP +A SH+ K+K AA+NLK
Sbjct: 315 --DIKTPPEIQGMIQDSCTKMSSELGKALKELALAIKRMTPPSSASSHLVKSKNAAKNLK 374

Query: 365 THLEEWRFEEVNNAMEIVHIVSLASLLFDTICCIEKIVDSVQKLASMAGFKAVEMQSSVA 424
             L       + N +E+V  V++ SLLF+ I C EKI +++ +LAS+A F+ VE +    
Sbjct: 375 FLLYSDLCSGI-NLLEVVPAVTVTSLLFEVISCTEKIAEAIHELASLAQFENVEQEKPKL 434

Query: 425 PQQQKDLQDEDSLQPCHGTTILLAHHVITVDEGVP 458
           P+Q +  Q         G  + + HHV+T+D+  P
Sbjct: 435 PEQGEMQQ---------GANMDVHHHVVTIDQPPP 452

BLAST of Lsi06G006300 vs. TrEMBL
Match: A0A0A0M0C7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G630860 PE=4 SV=1)

HSP 1 Score: 449.1 bits (1154), Expect = 6.3e-123
Identity = 246/451 (54.55%), Postives = 314/451 (69.62%), Query Frame = 1

Query: 5   VKPVFAKLLAMAVELATITKKLAKDDSRRVVHALKVGLAITLVSLFYYFKPLYDGFGTST 64
           +K   +K++A  +EL   TKKLAKDD RRVVH+LKVGLAITLVSLFYYF+PLYDG G S 
Sbjct: 19  LKAKISKIMAKVIELFKKTKKLAKDDPRRVVHSLKVGLAITLVSLFYYFEPLYDGLGASA 78

Query: 65  MWAIITVIVVFEFSVGGTLGRGLNRVMATFLAGALGFGAHYLASLGGEIGRPIMLGLFVF 124
           MWAI+TV+VVFEFS+G TLGRGLNRV+ATFLA ALGFGAH+LA L G+  +PIML L VF
Sbjct: 79  MWAILTVVVVFEFSIGATLGRGLNRVLATFLAAALGFGAHFLADLAGDTAQPIMLSLSVF 138

Query: 125 VLAGISTFTRFFPKMKARYDYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTILIGCFF 184
            LA I+TF RFFP++KARYDYG LIFILTFC+VSVSGYR+DEI K+AY R LTILIG F 
Sbjct: 139 FLAAITTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYREDEILKVAYRRALTILIGTFI 198

Query: 185 TLFVCIFVKPVWAGTDLHYLLITNIQSLAIFFQGFGVEYFGLSQEEEICKADMQKYRTIL 244
            + +CI + PVWAG DLH L+  NI+ LA FFQGFGVEY    +E+E     ++ ++++L
Sbjct: 199 AILICILICPVWAGDDLHSLVSNNIEQLANFFQGFGVEYSNEWKEDE---GIVEGFKSVL 258

Query: 245 NSKSSEESLSNLARWEPRHGKFRYRHPWNQYLEIGSLNRDCAYRLELLNGY-LKPNQFQV 304
            S+ +EESL N ARWEP HG F++RHPW QY +IGSL R CAYRLE LN Y L  +Q  +
Sbjct: 259 TSRQTEESLVNFARWEPGHGTFKFRHPWKQYRKIGSLTRQCAYRLESLNTYLLAESQTPL 318

Query: 305 RFNANFDPRLLELYRLDFYICSESSSGLRELALSLRKMVPPLTAKSHIEKAKIAAENLKT 364
                      ++        +ES   L++LA S+R M  P     HIEK+K AA++LK 
Sbjct: 319 HIRDQLKESCSKM-------STESGKALKDLASSIRTMTLPRLPNPHIEKSKAAAKDLKA 378

Query: 365 HLEEWRFEEVNNAMEIVHIVSLASLLFDTICCIEKIVDSVQKLASMAGFKAVEMQSSVAP 424
            L+        + +EIV + ++ASLL D+I CIEKI +SV +LAS+A FK  E++ S + 
Sbjct: 379 ALKIRPCNSSIDLLEIVPMATVASLLIDSISCIEKIAESVGELASLANFKRFEVEKSASL 438

Query: 425 QQQKDLQDEDSLQPCHGTTILLAH-HVITVD 454
           + Q++ Q     Q      I+  H HV+T+D
Sbjct: 439 KFQQEQQ-----QKLATPAIVSGHCHVVTID 454

BLAST of Lsi06G006300 vs. TrEMBL
Match: A0A067F4Z2_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g042529mg PE=4 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 1.1e-122
Identity = 237/433 (54.73%), Postives = 311/433 (71.82%), Query Frame = 1

Query: 5   VKPVFAKLLAMAVELATITKKLAKDDSRRVVHALKVGLAITLVSLFYYFKPLYDGFGTST 64
           +K +  K ++  +ELA  TKKL +DD RR++H+LKVGLAITLVSLFYYF+PLYDGFG S 
Sbjct: 23  LKSLPGKSMSKLIELAKKTKKLGQDDPRRIIHSLKVGLAITLVSLFYYFEPLYDGFGVSA 82

Query: 65  MWAIITVIVVFEFSVGGTLGRGLNRVMATFLAGALGFGAHYLASLGGEIGRPIMLGLFVF 124
           MWA++TV+VVFEFSVGGTL RGLNR +ATFLA  LGFGAH+LA+L G++G PI+LGLFVF
Sbjct: 83  MWAVLTVVVVFEFSVGGTLSRGLNRGLATFLAAGLGFGAHHLANLSGKLGEPILLGLFVF 142

Query: 125 VLAGISTFTRFFPKMKARYDYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTILIGCFF 184
           +LA   TF RFFP+MKARYDYGLLIFILTFC++SVSGYRDDE+  +A+ R+ TILIG F 
Sbjct: 143 LLASTVTFVRFFPRMKARYDYGLLIFILTFCLISVSGYRDDEVWHMAHMRVSTILIGGFT 202

Query: 185 TLFVCIFVKPVWAGTDLHYLLITNIQSLAIFFQGFGVEYFGLSQEEEICKADMQKYRTIL 244
            + VCI + PVWAGTDLH L+  NI  LA F +GFG  YF + Q+ E+    ++ Y+ +L
Sbjct: 203 AVCVCILIFPVWAGTDLHNLVANNIDKLANFLEGFGPLYFQIPQDGEMDMTFLEGYKCVL 262

Query: 245 NSKSSEESLSNLARWEPRHGKFRYRHPWNQYLEIGSLNRDCAYRLELLNGYLKPNQFQVR 304
           NSK +EESL+N A WEP HG+FR+RHPW QYL+IGSL R+CAYR+E LNGYL        
Sbjct: 263 NSKQTEESLANFAGWEPGHGEFRFRHPWKQYLKIGSLTRNCAYRIEALNGYL-------- 322

Query: 305 FNANFDPRLLELYR-----LDFYICSESSSGLRELALSLRKMVPPLTAKSHIEKAKIAAE 364
              N + ++ E  R         +  E+   L+ELA S++ M  P +A SHI K+KIAA+
Sbjct: 323 ---NTETKIPEEIRGKMQDACINMSYEAVKALKELAFSIQTMTKPCSANSHITKSKIAAK 382

Query: 365 NLKTHLEEWRFEEVNNAMEIVHIVSLASLLFDTICCIEKIVDSVQKLASMAGFKAVEMQS 424
           NLK+ L     +E    ++++  V++ASLL D + C EKI +SVQ+LAS+A FK+V+ + 
Sbjct: 383 NLKSLLSTSLCKE-TEILDLIPAVTVASLLVDAVACTEKIAESVQELASIAKFKSVKPKK 442

Query: 425 SVAPQQQKDLQDE 433
           + +    K  + E
Sbjct: 443 APSRSSSKISEPE 443

BLAST of Lsi06G006300 vs. TrEMBL
Match: V4SJW4_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10028411mg PE=4 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 1.1e-122
Identity = 237/433 (54.73%), Postives = 311/433 (71.82%), Query Frame = 1

Query: 5   VKPVFAKLLAMAVELATITKKLAKDDSRRVVHALKVGLAITLVSLFYYFKPLYDGFGTST 64
           +K +  K ++  +ELA  TKKL +DD RR++H+LKVGLAITLVSLFYYF+PLYDGFG S 
Sbjct: 23  LKSLPGKSMSKLIELAKKTKKLGQDDPRRIIHSLKVGLAITLVSLFYYFEPLYDGFGVSA 82

Query: 65  MWAIITVIVVFEFSVGGTLGRGLNRVMATFLAGALGFGAHYLASLGGEIGRPIMLGLFVF 124
           MWA++TV+VVFEFSVGGTL RGLNR +ATFLA  LGFGAH+LA+L G++G PI+LGLFVF
Sbjct: 83  MWAVLTVVVVFEFSVGGTLSRGLNRGLATFLAAGLGFGAHHLANLSGKLGEPILLGLFVF 142

Query: 125 VLAGISTFTRFFPKMKARYDYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTILIGCFF 184
           +LA   TF RFFP+MKARYDYGLLIFILTFC++SVSGYRDDE+  +A+ R+ TILIG F 
Sbjct: 143 LLASTVTFVRFFPRMKARYDYGLLIFILTFCLISVSGYRDDEVWHMAHMRVSTILIGGFT 202

Query: 185 TLFVCIFVKPVWAGTDLHYLLITNIQSLAIFFQGFGVEYFGLSQEEEICKADMQKYRTIL 244
            + VCI + PVWAGTDLH L+  NI  LA F +GFG  YF + Q+ E+    ++ Y+ +L
Sbjct: 203 AVCVCILIFPVWAGTDLHNLVANNIDKLANFLEGFGPLYFQIPQDGEMDMTFLEGYKCVL 262

Query: 245 NSKSSEESLSNLARWEPRHGKFRYRHPWNQYLEIGSLNRDCAYRLELLNGYLKPNQFQVR 304
           NSK +EESL+N A WEP HG+FR+RHPW QYL+IGSL R+CAYR+E LNGYL        
Sbjct: 263 NSKQTEESLANFAGWEPGHGEFRFRHPWKQYLKIGSLTRNCAYRIEALNGYL-------- 322

Query: 305 FNANFDPRLLELYR-----LDFYICSESSSGLRELALSLRKMVPPLTAKSHIEKAKIAAE 364
              N + ++ E  R         +  E+   L+ELA S++ M  P +A SHI K+KIAA+
Sbjct: 323 ---NTETKIPEEIRGKMQDACINMSYEAVKALKELAFSIQTMTKPCSANSHITKSKIAAK 382

Query: 365 NLKTHLEEWRFEEVNNAMEIVHIVSLASLLFDTICCIEKIVDSVQKLASMAGFKAVEMQS 424
           NLK+ L     +E    ++++  V++ASLL D + C EKI +SVQ+LAS+A FK+V+ + 
Sbjct: 383 NLKSLLSTSLCKE-TEILDLIPAVTVASLLVDAVACTEKIAESVQELASIAKFKSVKPKK 442

Query: 425 SVAPQQQKDLQDE 433
           + +    K  + E
Sbjct: 443 APSRSSSKISEPE 443

BLAST of Lsi06G006300 vs. TAIR10
Match: AT1G08440.1 (AT1G08440.1 Aluminium activated malate transporter family protein)

HSP 1 Score: 367.9 bits (943), Expect = 9.4e-102
Identity = 198/437 (45.31%), Postives = 290/437 (66.36%), Query Frame = 1

Query: 18  ELATITKKLAKDDSRRVVHALKVGLAITLVSLFYYFKPLYDGFGTSTMWAIITVIVVFEF 77
           E+    +++ K+D RRVVHA KVGLA+ LVS FYY++PLYD FG + MWA++TV+VVFEF
Sbjct: 6   EIVREGRRVGKEDPRRVVHAFKVGLALALVSSFYYYQPLYDNFGVNAMWAVMTVVVVFEF 65

Query: 78  SVGGTLGRGLNRVMATFLAGALGFGAHYLASLGGEIGRPIMLGLFVFVLAGISTFTRFFP 137
           SVG TLG+GLNR +AT +AG LG GAH+LASL G    PI+L +FVFVLA +STF RFFP
Sbjct: 66  SVGATLGKGLNRAVATLVAGGLGIGAHHLASLSGPTVEPILLAIFVFVLAALSTFVRFFP 125

Query: 138 KMKARYDYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTILIGCFFTLFVCIFVKPVWA 197
           ++KARYDYG+LIFILTF ++SVSG+R+DEI  LA+ R+ T+++G    + + IFV PVWA
Sbjct: 126 RVKARYDYGVLIFILTFALISVSGFREDEILDLAHKRLSTVIMGGVSCVLISIFVCPVWA 185

Query: 198 GTDLHYLLITNIQSLAIFFQGFGVEYFGLSQEEEICKAD-----MQKYRTILNSKSSEES 257
           G DLH LL +N  +L+ F Q FG EYF  +++ +I + +     +++Y+++LNSKS+EE+
Sbjct: 186 GQDLHSLLASNFDTLSHFLQEFGDEYFEATEDGDIKEVEKRRRNLERYKSVLNSKSNEEA 245

Query: 258 LSNLARWEPRHGKFRYRHPWNQYLEIGSLNRDCAYRLELLNGYLKPNQFQVRFNANFDPR 317
           L+N A+WEPRHG+FR+RHPW QYL +G+L R  AYR++ LN  +  +   ++   +   +
Sbjct: 246 LANFAKWEPRHGQFRFRHPWRQYLAVGALLRQSAYRIDALNSNINSD---MQIPMDIKKK 305

Query: 318 LLELYRLDFYICSESSSGLRELALSLRKMVPPLTAKSHIEKAKIAAENLKTHLEEWRFEE 377
           + E  R    + SES   ++E+++SL+ M    +   H+  ++ A + L T L+     +
Sbjct: 306 IEEPLR---RMSSESGKSMKEVSISLKNMTISSSFDIHVVNSQSACKTLSTLLKSGILND 365

Query: 378 VNNAMEIVHIVSLASLLFDTICCIEKIVDSVQKLASMAGFKAVEMQS-----SVAPQQQK 437
           V   ++++ +++  SLL D +   EKI +SV +LAS A FK  +  S     S+      
Sbjct: 366 V-EPLQMISLMTTVSLLIDIVNLTEKISESVHELASAAKFKNKKKPSKSNSGSIGQAMPN 425

Query: 438 DLQDEDSLQPCHGTTIL 445
              D+D     H  TIL
Sbjct: 426 KSHDDDD----HVVTIL 431

BLAST of Lsi06G006300 vs. TAIR10
Match: AT2G27240.1 (AT2G27240.1 Aluminium activated malate transporter family protein)

HSP 1 Score: 365.2 bits (936), Expect = 6.1e-101
Identity = 196/428 (45.79%), Postives = 272/428 (63.55%), Query Frame = 1

Query: 18  ELATITKKLAKDDSRRVVHALKVGLAITLVSLFYYFKPLYDGFGTSTMWAIITVIVVFEF 77
           E+    +++AK+D RRVVH+ KVGL + LVS FYY++PLYD FG + MWA++TV+VVFEF
Sbjct: 6   EIVREGRRVAKEDPRRVVHSFKVGLVLALVSSFYYYQPLYDSFGVNAMWAVMTVVVVFEF 65

Query: 78  SVGGTLGRGLNRVMATFLAGALGFGAHYLASLGGEIGRPIMLGLFVFVLAGISTFTRFFP 137
           SVG TLG+GLNRV AT  AG LG GAH+LAS+ G  G PI+L +FVFV A +STF RFFP
Sbjct: 66  SVGATLGKGLNRVAATLFAGGLGIGAHHLASMSGPTGEPILLAVFVFVQAALSTFVRFFP 125

Query: 138 KMKARYDYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTILIGCFFTLFVCIFVKPVWA 197
           ++KARYDY LLIFILTF ++SVSG+R++++ KL + RI T++IG    + + IFV PVWA
Sbjct: 126 RVKARYDYSLLIFILTFALISVSGFREEQVVKLTHKRISTVIIGGLSCVIISIFVCPVWA 185

Query: 198 GTDLHYLLITNIQSLAIFFQG--------------------------FGVEYFGL----- 257
           G DLH L+ +N + L+ F  G                          FG +Y  +     
Sbjct: 186 GQDLHSLIASNFEKLSFFLLGNSFHYVSSDLNSITLLRKIKSWRLADFGDKYCEVVENDG 245

Query: 258 SQEEEICKADMQKYRTILNSKSSEESLSNLARWEPRHGKFRYRHPWNQYLEIGSLNRDCA 317
           ++E +  K D   Y+++LNSKS+EESL+N A+WEP HG+FR+RHPW QYL +G L R CA
Sbjct: 246 AKEVDKRKKDFDNYKSVLNSKSNEESLANFAKWEPGHGQFRFRHPWKQYLAVGELIRQCA 305

Query: 318 YRLELLNGYLK-PNQFQVRFNANFDPRLLELYRLDFYICSESSSGLRELALSLRKMVPPL 377
           YR+  LN YL   NQ  V         L  +         ES   ++E+++SL+KM  P 
Sbjct: 306 YRIHALNSYLNADNQVSVDIKKKLGEPLRRM-------SLESGKAMKEMSISLKKMTKPS 365

Query: 378 TAKSHIEKAKIAAENLKTHLEEWRFEEVNNAMEIVHIVSLASLLFDTICCIEKIVDSVQK 414
           ++  H++ AK A ++L   L     +EV   +E+V +++  SLL D I   EKI++S+ +
Sbjct: 366 SSDLHVQNAKSACKSLTNLLNSGILKEV-EPLELVSLLTAISLLIDIINLTEKILESLHE 425

BLAST of Lsi06G006300 vs. TAIR10
Match: AT3G11680.1 (AT3G11680.1 Aluminium activated malate transporter family protein)

HSP 1 Score: 353.6 bits (906), Expect = 1.8e-97
Identity = 187/391 (47.83%), Postives = 263/391 (67.26%), Query Frame = 1

Query: 24  KKLAKDDSRRVVHALKVGLAITLVSLFYYFKPLYDGFGTSTMWAIITVIVVFEFSVGGTL 83
           +K AKDD RR++H++KVG+A+TLVSL YY +PLY  FG + MWAI+TV+VVFEF+VGGTL
Sbjct: 35  QKFAKDDPRRIIHSMKVGVALTLVSLLYYVRPLYISFGVTGMWAILTVVVVFEFTVGGTL 94

Query: 84  GRGLNRVMATFLAGALGFGAHYLASLGGEIGRPIMLGLFVFVLAGISTFTRFFPKMKARY 143
            +GLNR  AT +AGALG GA +LA   G  G PI+LG+ VF L   +TF+RFFP++K RY
Sbjct: 95  SKGLNRGFATLIAGALGVGAVHLARFFGHQGEPIVLGILVFSLGAAATFSRFFPRIKQRY 154

Query: 144 DYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTILIGCFFTLFVCIFVKPVWAGTDLHY 203
           DYG LIFILTF  V++SGYR DEI  +AY R+ TILIG    + V IF+ PVWAG DLH 
Sbjct: 155 DYGALIFILTFSFVAISGYRTDEILIMAYQRLSTILIGGTICILVSIFICPVWAGEDLHK 214

Query: 204 LLITNIQSLAIFFQGFGVEYFGLSQEEEICKADMQKYRTILNSKSSEESLSNLARWEPRH 263
           ++  NI  LA + +GF  EYF   +  +   + +++Y++IL SKS+E+SL+NLARWEP H
Sbjct: 215 MIANNINKLAKYLEGFEGEYFQPEKISKETSSCVREYKSILTSKSTEDSLANLARWEPGH 274

Query: 264 GKFRYRHPWNQYLEIGSLNRDCAYRLELLNGYLKPNQFQVRFNANFDPRLLELYRLDFYI 323
           G+FR RHPW +YL+I  L R CA  LE+LNGY+  N    +    F+ ++ E       +
Sbjct: 275 GRFRLRHPWKKYLKIAGLVRQCAVHLEILNGYVLSND---KAPQEFESKIQEPITT---M 334

Query: 324 CSESSSGLRELALSLRKM-VPPLTAKSHIEKAKIAAENLKTHLEEWRFEEVNNAMEIVHI 383
             E    L+ +A S++ M        +HI+ +K A +NLK  L+    E   + +EI+  
Sbjct: 335 SREVGEALKAIAKSIKTMRNDSACVNAHIDNSKKAIKNLKIALKSSYPETYKDLLEIIPG 394

Query: 384 VSLASLLFDTICCIEKIVDSVQKLASMAGFK 414
           V++AS+L + + C+EKI ++V++ + +A FK
Sbjct: 395 VTMASILIEVVNCVEKIYEAVEEFSGLAHFK 419

BLAST of Lsi06G006300 vs. TAIR10
Match: AT1G08430.1 (AT1G08430.1 aluminum-activated malate transporter 1)

HSP 1 Score: 344.7 bits (883), Expect = 8.5e-95
Identity = 188/437 (43.02%), Postives = 279/437 (63.84%), Query Frame = 1

Query: 25  KLAKDDSRRVVHALKVGLAITLVSLFYYFKPL---YDGFGTSTMWAIITVIVVFEFSVGG 84
           ++  +D RR++HA KVGLA+ LVS FYY++P     D FG + MWA++TV+VVFEFSVG 
Sbjct: 13  RVGNEDPRRIIHAFKVGLALVLVSSFYYYQPFGPFTDYFGINAMWAVMTVVVVFEFSVGA 72

Query: 85  TLGRGLNRVMATFLAGALGFGAHYLASLGGEIGRPIMLGLFVFVLAGISTFTRFFPKMKA 144
           TLG+GLNR +AT +AG LG GAH LA L G    PI+L + VFV A +STF RFFP +K 
Sbjct: 73  TLGKGLNRGVATLVAGGLGIGAHQLARLSGATVEPILLVMLVFVQAALSTFVRFFPWVKT 132

Query: 145 RYDYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTILIGCFFTLFVCIFVKPVWAGTDL 204
           ++DYG+LIFILTF ++S+SG+RD+EI  LA SR+ T++IG    + + IFV PVWAG DL
Sbjct: 133 KFDYGILIFILTFALISLSGFRDEEIMDLAESRLSTVVIGGVSCILISIFVCPVWAGQDL 192

Query: 205 HYLLITNIQSLAIFFQGFGVEYF-----GLSQEEEICKADMQKYRTILNSKSSEESLSNL 264
           H LL +N  +L+ F Q FG EYF     G  +  E  K ++++Y+++L+SKS EE+L+N 
Sbjct: 193 HSLLASNFDTLSHFLQDFGDEYFEAREKGDYKVVEKRKKNLERYKSVLDSKSDEEALANY 252

Query: 265 ARWEPRHGKFRYRHPWNQYLEIGSLNRDCAYRLELLNGYLKPN-QFQVRFNANFDPRLLE 324
           A WEP HG+FR+RHPW QY+ +G+L R CAYR++ LN Y+  + Q  V      +  L  
Sbjct: 253 AEWEPPHGQFRFRHPWKQYVAVGALLRQCAYRIDALNSYINSDFQIPVDIKKKLETPLRR 312

Query: 325 LYRLDFYICSESSSGLRELALSLRKMVPPLTAKSHIEKAKIAAENLKTHLEEWRFEEVNN 384
           +        SES + ++E+++SL++M+   ++  H+  ++ A ++L T L+     +V  
Sbjct: 313 M-------SSESGNSMKEMSISLKQMIKSSSSDIHVSNSQAACKSLSTLLKSGILNDV-E 372

Query: 385 AMEIVHIVSLASLLFDTICCIEKIVDSVQKLASMAGFKAVEMQSSVAPQQQKDLQDEDSL 444
            ++++ +++  S+L D +   EKI +SV +LAS A FK     + + P    +  D  S+
Sbjct: 373 PLQMISLMTTVSMLIDIVNLTEKISESVHELASAARFK-----NKMRPTVLYEKSDSGSI 432

Query: 445 QPCHGTTILLAHHVITV 453
                      HHV+TV
Sbjct: 433 GRAMPIDSHEDHHVVTV 436

BLAST of Lsi06G006300 vs. TAIR10
Match: AT4G00910.1 (AT4G00910.1 Aluminium activated malate transporter family protein)

HSP 1 Score: 252.3 bits (643), Expect = 5.7e-67
Identity = 137/310 (44.19%), Postives = 196/310 (63.23%), Query Frame = 1

Query: 8   VFAKLLAMAVELATITKKLAK------DDSRRVVHALKVGLAITLVSLFYYFKPLYDGFG 67
           ++ K L M V +  + K + K      DD  +VVH LKVGLA++LVS+FYY +PLYDG G
Sbjct: 35  LWLKDLVMKVIMERVAKFMRKAWRIGADDPAKVVHCLKVGLALSLVSIFYYMRPLYDGVG 94

Query: 68  TSTMWAIITVIVVFEFSVGGTLGRGLNRVMATFLAGALGFGAHYLASLGGEIGRPIMLGL 127
            + MWAI+TV+VVFE +VG T  + +NRV+AT LAG+LG   H++A+  G+     ++G 
Sbjct: 95  GNAMWAIMTVVVVFESNVGATFCKCVNRVVATILAGSLGIAVHWVATQSGK-AEVFVIGC 154

Query: 128 FVFVLAGISTFTRFFPKMKARYDYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTILIG 187
            VF+ A  +T++RF P  KAR+DYG +IFILTF +VSV GYR D++ +LA  R+ TI IG
Sbjct: 155 SVFLFAFAATYSRFVPSFKARFDYGAMIFILTFSLVSVGGYRVDKLVELAQQRVSTIAIG 214

Query: 188 CFFTLFVCIFVKPVWAGTDLHYLLITNIQSLAIFFQGFGVEYFGLSQ------EEEICKA 247
               + + +F  P+WAG+ LH L+  N++ LA    G   EYF  ++      E+E    
Sbjct: 215 TSICIIITVFFCPIWAGSQLHRLIERNLEKLADSLDGCVAEYFKENEVSTNRNEDENTNM 274

Query: 248 DMQKYRTILNSKSSEESL------------SNLARWEPRHGKFRYRHPWNQYLEIGSLNR 294
            +Q ++ +LNSK +EE++            +NLARWEP HG F +RHPW  Y++IG+  R
Sbjct: 275 KLQGFKCVLNSKGTEEAMPLIRFSGFSFSQANLARWEPAHGSFNFRHPWKLYVKIGAAMR 334

BLAST of Lsi06G006300 vs. NCBI nr
Match: gi|449442739|ref|XP_004139138.1| (PREDICTED: aluminum-activated malate transporter 2-like [Cucumis sativus])

HSP 1 Score: 710.3 bits (1832), Expect = 2.2e-201
Identity = 366/460 (79.57%), Postives = 405/460 (88.04%), Query Frame = 1

Query: 5   VKPVFAKLLAMAVELATITKKLAKDDSRRVVHALKVGLAITLVSLFYYFKPLYDGFGTST 64
           +KP+FAKL  ++V+LAT+ KKLAKDDSRRVVHALKVGLAI+LVSL YYFKPLYDGFGTST
Sbjct: 21  LKPIFAKLSTVSVKLATMAKKLAKDDSRRVVHALKVGLAISLVSLLYYFKPLYDGFGTST 80

Query: 65  MWAIITVIVVFEFSVGGTLGRGLNRVMATFLAGALGFGAHYLASLGGEIGRPIMLGLFVF 124
           MWAI+TVIVVFEFSVGGTLGRGLNRVMAT LAG LGFG HYLASLGG+ GRPI+L LFVF
Sbjct: 81  MWAIVTVIVVFEFSVGGTLGRGLNRVMATLLAGGLGFGTHYLASLGGDTGRPIILALFVF 140

Query: 125 VLAGISTFTRFFPKMKARYDYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTILIGCFF 184
           +LA +STFTRFFPK+KARYDYGLLI ILTFCMVS+SGYRD+EIAKLA SRILTILIGC  
Sbjct: 141 ILASVSTFTRFFPKIKARYDYGLLILILTFCMVSLSGYRDEEIAKLALSRILTILIGCCV 200

Query: 185 TLFVCIFVKPVWAGTDLHYLLITNIQSLAIFFQGFGVEYFGLSQEEEICKADMQKYRTIL 244
           TL VCIFV+PVWAGTDLH L+  NIQSLA+FFQGFG E+FGLSQE E+   DMQKYRTIL
Sbjct: 201 TLIVCIFVRPVWAGTDLHCLVANNIQSLALFFQGFGAEFFGLSQEGEVSNDDMQKYRTIL 260

Query: 245 NSKSSEESLSNLARWEPRHGKFRYRHPWNQYLEIGSLNRDCAYRLELLNGYLKPNQFQV- 304
           NSKS+EESL+NLARWEPRHGKFRYRHPW QYL+IGSLNR+CAYRLELLNGYLK NQFQ+ 
Sbjct: 261 NSKSNEESLTNLARWEPRHGKFRYRHPWKQYLKIGSLNRECAYRLELLNGYLKTNQFQMP 320

Query: 305 --RFNANFDPRLLELYRLDFYICSESSSGLRELALSLRKMVPPLTAKSHIEKAKIAAENL 364
             + +  F    ++       ICSESS GLRELAL+LRKMV PLTAKSHIEKAKIAAENL
Sbjct: 321 SQQIHGQFKEECMK-------ICSESSRGLRELALALRKMVLPLTAKSHIEKAKIAAENL 380

Query: 365 KTHLEEWRFEEVNNAMEIVHIVSLASLLFDTICCIEKIVDSVQKLASMAGFKAVEMQSSV 424
           K+HLEEWRFEEVNNAMEIV +VSLASLLFDTICCIEKIVDSVQ+LASMAGFKAVE+QSSV
Sbjct: 381 KSHLEEWRFEEVNNAMEIVQVVSLASLLFDTICCIEKIVDSVQELASMAGFKAVEVQSSV 440

Query: 425 APQQQKDLQDED--SLQP-CHGTTILLAHHVITVDEGVPC 459
           AP+QQ DLQD+D  +LQP  HG  +LLAHH IT+DE  PC
Sbjct: 441 APEQQMDLQDQDQYALQPLSHGAAVLLAHHAITIDEQSPC 473

BLAST of Lsi06G006300 vs. NCBI nr
Match: gi|659098790|ref|XP_008450297.1| (PREDICTED: aluminum-activated malate transporter 2-like [Cucumis melo])

HSP 1 Score: 709.5 bits (1830), Expect = 3.7e-201
Identity = 368/456 (80.70%), Postives = 402/456 (88.16%), Query Frame = 1

Query: 5   VKPVFAKLLAMAVELATITKKLAKDDSRRVVHALKVGLAITLVSLFYYFKPLYDGFGTST 64
           +KP+FAKL   AV+LAT+TKKLAKDDSRRVVHA KVGLAI+LVSL YYFK LYDGFGTST
Sbjct: 21  LKPIFAKLSNKAVKLATMTKKLAKDDSRRVVHAFKVGLAISLVSLLYYFKALYDGFGTST 80

Query: 65  MWAIITVIVVFEFSVGGTLGRGLNRVMATFLAGALGFGAHYLASLGGEIGRPIMLGLFVF 124
           MWAI+TVIVV EFSVGGTLGRGLNRVMAT LAG LGFGAHYLASL G+ GRPI+L  FVF
Sbjct: 81  MWAIVTVIVVVEFSVGGTLGRGLNRVMATLLAGGLGFGAHYLASLSGDTGRPIILAFFVF 140

Query: 125 VLAGISTFTRFFPKMKARYDYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTILIGCFF 184
           +LA ISTFTRFFPK+KARYDYG+LIFILTFCMVS+SGYRDDEIAKLA SRILTILIGC F
Sbjct: 141 LLASISTFTRFFPKIKARYDYGMLIFILTFCMVSLSGYRDDEIAKLALSRILTILIGCCF 200

Query: 185 TLFVCIFVKPVWAGTDLHYLLITNIQSLAIFFQGFGVEYFGLSQEEEICKADMQKYRTIL 244
           TLFVCIFV+PVWAGTDLH L+  NIQSLA+FFQGFG E+F LSQ EE+ K DMQKYRTIL
Sbjct: 201 TLFVCIFVRPVWAGTDLHCLVANNIQSLALFFQGFGAEFFRLSQ-EEVSKDDMQKYRTIL 260

Query: 245 NSKSSEESLSNLARWEPRHGKFRYRHPWNQYLEIGSLNRDCAYRLELLNGYLKPNQFQVR 304
           NSKS+EESL+NLARWEPRHGKFRYRHPW QYL+IGSLNR+CAYRLELLNGYLK NQFQ+ 
Sbjct: 261 NSKSNEESLTNLARWEPRHGKFRYRHPWKQYLKIGSLNRECAYRLELLNGYLKTNQFQMP 320

Query: 305 FNANFDPRLLELYRLDFYICSESSSGLRELALSLRKMVPPLTAKSHIEKAKIAAENLKTH 364
                 P   E  +    ICSESS GLRELAL+LRKMV PLTAKSHIEKAK+AAE+LK+H
Sbjct: 321 SQQIHGPFKEECMK----ICSESSRGLRELALALRKMVLPLTAKSHIEKAKVAAEDLKSH 380

Query: 365 LEEWRFEEVNNAMEIVHIVSLASLLFDTICCIEKIVDSVQKLASMAGFKAVEMQSSVAPQ 424
           LEEWRFEEVNNAMEIV +VSLASLLFDTICCIEKIVDSVQ+LASMAGFKAVE+QSSVAP+
Sbjct: 381 LEEWRFEEVNNAMEIVQVVSLASLLFDTICCIEKIVDSVQELASMAGFKAVEVQSSVAPE 440

Query: 425 QQKDLQDED--SLQPCHGTTILLAHHVITVDEGVPC 459
           QQ DLQD+D  SLQP HG  +LLAHH IT+DE  PC
Sbjct: 441 QQMDLQDQDQYSLQPSHGAAVLLAHHAITIDEQSPC 471

BLAST of Lsi06G006300 vs. NCBI nr
Match: gi|224106690|ref|XP_002314249.1| (hypothetical protein POPTR_0009s02310g [Populus trichocarpa])

HSP 1 Score: 450.7 bits (1158), Expect = 3.1e-123
Identity = 245/455 (53.85%), Postives = 316/455 (69.45%), Query Frame = 1

Query: 5   VKPVFAKLLAMAVELATITKKLAKDDSRRVVHALKVGLAITLVSLFYYFKPLYDGFGTST 64
           +K +  +  A  VE A   KKL +DD RRV H++KVGLAITLVSLFYYF+PLYDGFG S 
Sbjct: 15  IKSLPERSKAKIVECARKIKKLGQDDPRRVNHSVKVGLAITLVSLFYYFEPLYDGFGDSA 74

Query: 65  MWAIITVIVVFEFSVGGTLGRGLNRVMATFLAGALGFGAHYLASLGGEIGRPIMLGLFVF 124
           MWA++TV+VVFEFSVG TLGRGLNR +ATFLAGALGFGAH LA+L GE G P++LGLFVF
Sbjct: 75  MWAVMTVVVVFEFSVGATLGRGLNRGLATFLAGALGFGAHRLATLSGEKGEPMLLGLFVF 134

Query: 125 VLAGISTFTRFFPKMKARYDYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTILIGCFF 184
           +LA   TF RFFP+MKARYDYGLLIFILTFC++SVSGYRDDE+  +A+ R+ TILIG   
Sbjct: 135 LLATTVTFVRFFPRMKARYDYGLLIFILTFCLISVSGYRDDEMLDMAHKRVSTILIGSLT 194

Query: 185 TLFVCIFVKPVWAGTDLHYLLITNIQSLAIFFQGFGVEYFGLSQE-EEICKADMQKYRTI 244
            +FVCI + PVWAG DLH L  TNI+ L IF + FGVE+F    E E I KA +Q Y+++
Sbjct: 195 AVFVCICICPVWAGDDLHNLAATNIEKLGIFLEHFGVEFFRKPGEGESINKASLQGYKSV 254

Query: 245 LNSKSSEESLSNLARWEPRHGKFRYRHPWNQYLEIGSLNRDCAYRLELLNGYLKPNQFQV 304
           LNSK+ EESL N ARWEP HG+F++RHPW  YL+ GSL R CAYR+E LNGYL       
Sbjct: 255 LNSKNMEESLVNFARWEPGHGQFKFRHPWKHYLKFGSLTRQCAYRVEALNGYLNS----- 314

Query: 305 RFNANFDPRLLELYRLD-FYICSESSSGLRELALSLRKMVPPLTAKSHIEKAKIAAENLK 364
             +    P +  + +     + SE    L+ELAL++++M PP +A SH+ K+K AA+NLK
Sbjct: 315 --DIKTPPEIQGMIQDSCTKMSSELGKALKELALAIKRMTPPSSASSHLVKSKNAAKNLK 374

Query: 365 THLEEWRFEEVNNAMEIVHIVSLASLLFDTICCIEKIVDSVQKLASMAGFKAVEMQSSVA 424
             L       + N +E+V  V++ SLLF+ I C EKI +++ +LAS+A F+ VE +    
Sbjct: 375 FLLYSDLCSGI-NLLEVVPAVTVTSLLFEVISCTEKIAEAIHELASLAQFENVEQEKPKL 434

Query: 425 PQQQKDLQDEDSLQPCHGTTILLAHHVITVDEGVP 458
           P+Q +  Q         G  + + HHV+T+D+  P
Sbjct: 435 PEQGEMQQ---------GANMDVHHHVVTIDQPPP 452

BLAST of Lsi06G006300 vs. NCBI nr
Match: gi|449442741|ref|XP_004139139.1| (PREDICTED: aluminum-activated malate transporter 2 [Cucumis sativus])

HSP 1 Score: 449.1 bits (1154), Expect = 9.1e-123
Identity = 246/451 (54.55%), Postives = 314/451 (69.62%), Query Frame = 1

Query: 5   VKPVFAKLLAMAVELATITKKLAKDDSRRVVHALKVGLAITLVSLFYYFKPLYDGFGTST 64
           +K   +K++A  +EL   TKKLAKDD RRVVH+LKVGLAITLVSLFYYF+PLYDG G S 
Sbjct: 19  LKAKISKIMAKVIELFKKTKKLAKDDPRRVVHSLKVGLAITLVSLFYYFEPLYDGLGASA 78

Query: 65  MWAIITVIVVFEFSVGGTLGRGLNRVMATFLAGALGFGAHYLASLGGEIGRPIMLGLFVF 124
           MWAI+TV+VVFEFS+G TLGRGLNRV+ATFLA ALGFGAH+LA L G+  +PIML L VF
Sbjct: 79  MWAILTVVVVFEFSIGATLGRGLNRVLATFLAAALGFGAHFLADLAGDTAQPIMLSLSVF 138

Query: 125 VLAGISTFTRFFPKMKARYDYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTILIGCFF 184
            LA I+TF RFFP++KARYDYG LIFILTFC+VSVSGYR+DEI K+AY R LTILIG F 
Sbjct: 139 FLAAITTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYREDEILKVAYRRALTILIGTFI 198

Query: 185 TLFVCIFVKPVWAGTDLHYLLITNIQSLAIFFQGFGVEYFGLSQEEEICKADMQKYRTIL 244
            + +CI + PVWAG DLH L+  NI+ LA FFQGFGVEY    +E+E     ++ ++++L
Sbjct: 199 AILICILICPVWAGDDLHSLVSNNIEQLANFFQGFGVEYSNEWKEDE---GIVEGFKSVL 258

Query: 245 NSKSSEESLSNLARWEPRHGKFRYRHPWNQYLEIGSLNRDCAYRLELLNGY-LKPNQFQV 304
            S+ +EESL N ARWEP HG F++RHPW QY +IGSL R CAYRLE LN Y L  +Q  +
Sbjct: 259 TSRQTEESLVNFARWEPGHGTFKFRHPWKQYRKIGSLTRQCAYRLESLNTYLLAESQTPL 318

Query: 305 RFNANFDPRLLELYRLDFYICSESSSGLRELALSLRKMVPPLTAKSHIEKAKIAAENLKT 364
                      ++        +ES   L++LA S+R M  P     HIEK+K AA++LK 
Sbjct: 319 HIRDQLKESCSKM-------STESGKALKDLASSIRTMTLPRLPNPHIEKSKAAAKDLKA 378

Query: 365 HLEEWRFEEVNNAMEIVHIVSLASLLFDTICCIEKIVDSVQKLASMAGFKAVEMQSSVAP 424
            L+        + +EIV + ++ASLL D+I CIEKI +SV +LAS+A FK  E++ S + 
Sbjct: 379 ALKIRPCNSSIDLLEIVPMATVASLLIDSISCIEKIAESVGELASLANFKRFEVEKSASL 438

Query: 425 QQQKDLQDEDSLQPCHGTTILLAH-HVITVD 454
           + Q++ Q     Q      I+  H HV+T+D
Sbjct: 439 KFQQEQQ-----QKLATPAIVSGHCHVVTID 454

BLAST of Lsi06G006300 vs. NCBI nr
Match: gi|567862914|ref|XP_006424111.1| (hypothetical protein CICLE_v10028411mg [Citrus clementina])

HSP 1 Score: 448.4 bits (1152), Expect = 1.5e-122
Identity = 237/433 (54.73%), Postives = 311/433 (71.82%), Query Frame = 1

Query: 5   VKPVFAKLLAMAVELATITKKLAKDDSRRVVHALKVGLAITLVSLFYYFKPLYDGFGTST 64
           +K +  K ++  +ELA  TKKL +DD RR++H+LKVGLAITLVSLFYYF+PLYDGFG S 
Sbjct: 23  LKSLPGKSMSKLIELAKKTKKLGQDDPRRIIHSLKVGLAITLVSLFYYFEPLYDGFGVSA 82

Query: 65  MWAIITVIVVFEFSVGGTLGRGLNRVMATFLAGALGFGAHYLASLGGEIGRPIMLGLFVF 124
           MWA++TV+VVFEFSVGGTL RGLNR +ATFLA  LGFGAH+LA+L G++G PI+LGLFVF
Sbjct: 83  MWAVLTVVVVFEFSVGGTLSRGLNRGLATFLAAGLGFGAHHLANLSGKLGEPILLGLFVF 142

Query: 125 VLAGISTFTRFFPKMKARYDYGLLIFILTFCMVSVSGYRDDEIAKLAYSRILTILIGCFF 184
           +LA   TF RFFP+MKARYDYGLLIFILTFC++SVSGYRDDE+  +A+ R+ TILIG F 
Sbjct: 143 LLASTVTFVRFFPRMKARYDYGLLIFILTFCLISVSGYRDDEVWHMAHMRVSTILIGGFT 202

Query: 185 TLFVCIFVKPVWAGTDLHYLLITNIQSLAIFFQGFGVEYFGLSQEEEICKADMQKYRTIL 244
            + VCI + PVWAGTDLH L+  NI  LA F +GFG  YF + Q+ E+    ++ Y+ +L
Sbjct: 203 AVCVCILIFPVWAGTDLHNLVANNIDKLANFLEGFGPLYFQIPQDGEMDMTFLEGYKCVL 262

Query: 245 NSKSSEESLSNLARWEPRHGKFRYRHPWNQYLEIGSLNRDCAYRLELLNGYLKPNQFQVR 304
           NSK +EESL+N A WEP HG+FR+RHPW QYL+IGSL R+CAYR+E LNGYL        
Sbjct: 263 NSKQTEESLANFAGWEPGHGEFRFRHPWKQYLKIGSLTRNCAYRIEALNGYL-------- 322

Query: 305 FNANFDPRLLELYR-----LDFYICSESSSGLRELALSLRKMVPPLTAKSHIEKAKIAAE 364
              N + ++ E  R         +  E+   L+ELA S++ M  P +A SHI K+KIAA+
Sbjct: 323 ---NTETKIPEEIRGKMQDACINMSYEAVKALKELAFSIQTMTKPCSANSHITKSKIAAK 382

Query: 365 NLKTHLEEWRFEEVNNAMEIVHIVSLASLLFDTICCIEKIVDSVQKLASMAGFKAVEMQS 424
           NLK+ L     +E    ++++  V++ASLL D + C EKI +SVQ+LAS+A FK+V+ + 
Sbjct: 383 NLKSLLSTSLCKE-TEILDLIPAVTVASLLVDAVACTEKIAESVQELASIAKFKSVKPKK 442

Query: 425 SVAPQQQKDLQDE 433
           + +    K  + E
Sbjct: 443 APSRSSSKISEPE 443

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
ALMT2_ARATH1.7e-10045.31Aluminum-activated malate transporter 2 OS=Arabidopsis thaliana GN=ALMT2 PE=2 SV... [more]
ALMT7_ARATH1.1e-9945.79Aluminum-activated malate transporter 7 OS=Arabidopsis thaliana GN=ALMT7 PE=3 SV... [more]
ALMT8_ARATH3.2e-9647.83Aluminum-activated malate transporter 8 OS=Arabidopsis thaliana GN=ALMT8 PE=3 SV... [more]
ALMT1_ARATH1.5e-9343.02Aluminum-activated malate transporter 1 OS=Arabidopsis thaliana GN=ALMT1 PE=1 SV... [more]
ALMT1_WHEAT4.0e-7840.79Aluminum-activated malate transporter 1 OS=Triticum aestivum GN=ALMT1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0M301_CUCSA1.5e-20179.57Uncharacterized protein OS=Cucumis sativus GN=Csa_1G630850 PE=4 SV=1[more]
B9HQ62_POPTR2.2e-12353.85Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0009s02310g PE=4 SV=1[more]
A0A0A0M0C7_CUCSA6.3e-12354.55Uncharacterized protein OS=Cucumis sativus GN=Csa_1G630860 PE=4 SV=1[more]
A0A067F4Z2_CITSI1.1e-12254.73Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g042529mg PE=4 SV=1[more]
V4SJW4_9ROSI1.1e-12254.73Uncharacterized protein OS=Citrus clementina GN=CICLE_v10028411mg PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G08440.19.4e-10245.31 Aluminium activated malate transporter family protein[more]
AT2G27240.16.1e-10145.79 Aluminium activated malate transporter family protein[more]
AT3G11680.11.8e-9747.83 Aluminium activated malate transporter family protein[more]
AT1G08430.18.5e-9543.02 aluminum-activated malate transporter 1[more]
AT4G00910.15.7e-6744.19 Aluminium activated malate transporter family protein[more]
Match NameE-valueIdentityDescription
gi|449442739|ref|XP_004139138.1|2.2e-20179.57PREDICTED: aluminum-activated malate transporter 2-like [Cucumis sativus][more]
gi|659098790|ref|XP_008450297.1|3.7e-20180.70PREDICTED: aluminum-activated malate transporter 2-like [Cucumis melo][more]
gi|224106690|ref|XP_002314249.1|3.1e-12353.85hypothetical protein POPTR_0009s02310g [Populus trichocarpa][more]
gi|449442741|ref|XP_004139139.1|9.1e-12354.55PREDICTED: aluminum-activated malate transporter 2 [Cucumis sativus][more]
gi|567862914|ref|XP_006424111.1|1.5e-12254.73hypothetical protein CICLE_v10028411mg [Citrus clementina][more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0015743malate transport
Vocabulary: INTERPRO
TermDefinition
IPR020966ALMT
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015743 malate transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009705 plant-type vacuole membrane
cellular_component GO:0005575 cellular_component
molecular_function GO:0015140 malate transmembrane transporter activity
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi06G006300.1Lsi06G006300.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020966Aluminum-activated malate transporterPFAMPF11744ALMTcoord: 25..366
score: 9.9E
NoneNo IPR availableunknownCoilCoilcoord: 351..371
scor
NoneNo IPR availablePANTHERPTHR31086FAMILY NOT NAMEDcoord: 3..439
score: 3.3E
NoneNo IPR availablePANTHERPTHR31086:SF22ALUMINUM-ACTIVATED MALATE TRANSPORTER 1-RELATEDcoord: 3..439
score: 3.3E

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Lsi06G006300CsaV3_1G043740Cucumber (Chinese Long) v3cuclsiB068
The following gene(s) are paralogous to this gene:

None