Lsi06G005930 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi06G005930
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	ralf-like 9
Location	chr06 : 8118079 .. 8119191 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACAACAGTTCTTCCCAAAGATTTCATATTGCTTGGTTATGTAACATATTTAAAAGTACCTAAAAAAACTTATAAATGCTTAAAAGCATTGACGTTTGAAAAAAAATGCTTAAAAGCATTTCAAAAACACACTTGGAATTTAGTCTCTAAATTCAAAGTGTATGCAACATGACCATTCGGATAAAGAACACTACCCTAAATAATGAGTAAATTAACAAAGTCCAACATTAGTTATGGAAGGGAGAGATTTATGGGTATATCAGTAAGGATAATTATTTTCATTCTCCTAGAGATATGTGGAGTTTTGTTTCCCTTGTCATTATACTTTCTAGTTGTTTAATTTATATGCACAAGAACAAGAATTGGATCGTAAAATGTTTTATTATTCTCTTAACACTTGTACGCTTTCTTTCCCCACCTATATTATGTAATTTTGTTATTGTATGAATATATTGTCAAAAGGATAGTCTTACATGCTAACAAAATGTTTTTGCAACAAATATGCTATTTTTTTTTCAAACAAATTTAAGATTGTTTTGTTAGCACTACTTATTTTTGTAATTTCCTGTTTAAGGAATAAAACATTGTTGTCATTTGGTTTAACCTACAATGTTGGCAAAATATTAACCATAACATTGGAACGTGTTCTTCCTCGGTAAGATTCTAACAAGATAAACATATGCAACAATTAGTAAAACCACCTTATATTAAAAATGGCTATTTTAGATATATAAATATTCTTATCTTTTGAAGTCTACCCTATCGCAAGTGCCTCACGTTTTAAGTTTTATAATAGATTTTGTTATCAATGATGACCATGTCAAAGGGAATCACACTTGTTTTTATTGGGATTCTCTTGGTTAACTTTGACATGATTGAGGTTACAAATGCAACAGATTACATTGGTTATGATCCAATAAAAGGAGATATTAGTCCTGGATGTAGTAAAAAGCATCCAGAACTTTGTAAGTTAACTCCTGCAAATCCATATCAAAGAGGTTGCAGTGCAATCAATCGTTGCAGAGGAGGAAATGATATTATAGATGGTGTAGAAGAGGTTGCATCCACTAGCCCCGCAATGAGCCCTAATGCGGAACTGCATCTTCATTAA

mRNA sequence

ATGACAACAGTTCTTCCCAAAGATTTCATATTGCTTGGTTATGGAATCACACTTGTTTTTATTGGGATTCTCTTGGTTAACTTTGACATGATTGAGGTTACAAATGCAACAGATTACATTGGTTATGATCCAATAAAAGGAGATATTAGTCCTGGATGTAGTAAAAAGCATCCAGAACTTTGTAAGTTAACTCCTGCAAATCCATATCAAAGAGGTTGCAGTGCAATCAATCGTTGCAGAGGAGGAAATGATATTATAGATGGTGTAGAAGAGGTTGCATCCACTAGCCCCGCAATGAGCCCTAATGCGGAACTGCATCTTCATTAA

Coding sequence (CDS)

ATGACAACAGTTCTTCCCAAAGATTTCATATTGCTTGGTTATGGAATCACACTTGTTTTTATTGGGATTCTCTTGGTTAACTTTGACATGATTGAGGTTACAAATGCAACAGATTACATTGGTTATGATCCAATAAAAGGAGATATTAGTCCTGGATGTAGTAAAAAGCATCCAGAACTTTGTAAGTTAACTCCTGCAAATCCATATCAAAGAGGTTGCAGTGCAATCAATCGTTGCAGAGGAGGAAATGATATTATAGATGGTGTAGAAGAGGTTGCATCCACTAGCCCCGCAATGAGCCCTAATGCGGAACTGCATCTTCATTAA

Protein sequence

MTTVLPKDFILLGYGITLVFIGILLVNFDMIEVTNATDYIGYDPIKGDISPGCSKKHPELCKLTPANPYQRGCSAINRCRGGNDIIDGVEEVASTSPAMSPNAELHLH

Homology

[Close]

BLAST of Lsi06G005930 vs. Swiss-Prot
Match: RLF9_ARATH (Protein RALF-like 9 OS=Arabidopsis thaliana GN=RALFL9 PE=3 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 2.1e-09
Identity = 34/67 (50.75%), Postives = 39/67 (58.21%), Query Frame = 1


Query: 19 VFIGILLVNFDMIEVTN--ATDYIGYDPI-KGDISPGCSKKHPELCKLTPANPYQRGCSA 78
          V + + LV F  +  T   AT YI Y  I +GD +  C K HP  CK   ANPYQRGC  
Sbjct: 9  VILSLALVVFLALAATKVEATRYITYPAIDRGDHAVHCDKAHPNTCKKKEANPYQRGCEK 68

Query: 79 INRCRGG 83
          INRCRGG
Sbjct: 69 INRCRGG 75

[Close]

BLAST of Lsi06G005930 vs. Swiss-Prot
Match: RLF15_ARATH (Protein RALF-like 15 OS=Arabidopsis thaliana GN=RALFL15 PE=3 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 2.8e-06
Identity = 28/65 (43.08%), Postives = 35/65 (53.85%), Query Frame = 1


Query: 19 VFIGILLVNFDMIEVTN--ATDYIGYDPIK-GDISPGCSKKHPELCKLTPANPYQRGCSA 78
          V + + L+ F  +  T   AT YI Y  +  GD +  C K HP  CK   ANPY+RGC  
Sbjct: 9  VIVSLALILFLALAATKVEATRYISYRGMNHGDHAIHCDKAHPNTCKKQVANPYRRGCGT 68

Query: 79 INRCR 81
          I RCR
Sbjct: 69 IERCR 73

[Close]

BLAST of Lsi06G005930 vs. Swiss-Prot
Match: RLF29_ARATH (Protein RALF-like 29 OS=Arabidopsis thaliana GN=RALFL29 PE=3 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 6.2e-06
Identity = 28/65 (43.08%), Postives = 37/65 (56.92%), Query Frame = 1


Query: 18 LVFIGILLVNFDMIEVTNATDYIGYDPIKGDISPGCSKKHPELC--KLTPANPYQRGCSA 77
          + F+ IL+V    I   +A  YI Y PI+ D+  GC  + P     K TPANPY+R C+ 
Sbjct: 7  VTFVTILIVLCVFISTIHAKRYIEY-PIRLDLGKGCDPRFPTAACYKRTPANPYRRPCTT 66

Query: 78 INRCR 81
           NRCR
Sbjct: 67 ANRCR 70

[Close]

BLAST of Lsi06G005930 vs. TrEMBL
Match: A0A0A0L8B1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G426350 PE=4 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 1.3e-23
Identity = 60/100 (60.00%), Postives = 74/100 (74.00%), Query Frame = 1


Query: 10  ILLGYGITLVFIGILLVNFDMIEVTNATDYIGYDPI-KGDISPGCSKKHPELCKLTPANP 69
           +++   +TLVFIGILLV+FDMI +TNA  +I Y  I  GD+SPGCS  HPELC++  ANP
Sbjct: 1   MIMSKRMTLVFIGILLVSFDMIMITNARKFIDYGSIVAGDVSPGCSPTHPELCRVKSANP 60

Query: 70  YQRGCSAINRCRGGNDIIDGVEE----VASTSPAMSPNAE 105
           YQRGC+ I+RCR GNDIID  EE     AS SP++SPN E
Sbjct: 61  YQRGCNRIDRCREGNDIIDAEEEHIEGDASISPSISPNIE 100

[Close]

BLAST of Lsi06G005930 vs. TrEMBL
Match: D7KVJ4_ARALL (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_893358 PE=4 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 9.7e-06
Identity = 26/49 (53.06%), Postives = 30/49 (61.22%), Query Frame = 1


Query: 36 ATDYIGYDPIK-GDISPGCSKKHPELCKLTPANPYQRGCSAINRCRGGN 84
          AT YI Y  I  GD +  C K HP  CK   ANPY+RGC  + RCRGG+
Sbjct: 28 ATRYITYPSINHGDHATHCDKAHPNTCKKKEANPYKRGCEILERCRGGS 76

[Close]

BLAST of Lsi06G005930 vs. TAIR10
Match: AT1G61566.1 (AT1G61566.1 ralf-like 9)

HSP 1 Score: 63.2 bits (152), Expect = 1.2e-10
Identity = 34/67 (50.75%), Postives = 39/67 (58.21%), Query Frame = 1


Query: 19 VFIGILLVNFDMIEVTN--ATDYIGYDPI-KGDISPGCSKKHPELCKLTPANPYQRGCSA 78
          V + + LV F  +  T   AT YI Y  I +GD +  C K HP  CK   ANPYQRGC  
Sbjct: 9  VILSLALVVFLALAATKVEATRYITYPAIDRGDHAVHCDKAHPNTCKKKEANPYQRGCEK 68

Query: 79 INRCRGG 83
          INRCRGG
Sbjct: 69 INRCRGG 75

[Close]

BLAST of Lsi06G005930 vs. TAIR10
Match: AT2G22055.1 (AT2G22055.1 RALF-like 15)

HSP 1 Score: 52.8 bits (125), Expect = 1.6e-07
Identity = 28/65 (43.08%), Postives = 35/65 (53.85%), Query Frame = 1


Query: 19 VFIGILLVNFDMIEVTN--ATDYIGYDPIK-GDISPGCSKKHPELCKLTPANPYQRGCSA 78
          V + + L+ F  +  T   AT YI Y  +  GD +  C K HP  CK   ANPY+RGC  
Sbjct: 9  VIVSLALILFLALAATKVEATRYISYRGMNHGDHAIHCDKAHPNTCKKQVANPYRRGCGT 68

Query: 79 INRCR 81
          I RCR
Sbjct: 69 IERCR 73

[Close]

BLAST of Lsi06G005930 vs. TAIR10
Match: AT4G11653.1 (AT4G11653.1 RALF-like 29)

HSP 1 Score: 51.6 bits (122), Expect = 3.5e-07
Identity = 28/65 (43.08%), Postives = 37/65 (56.92%), Query Frame = 1


Query: 18 LVFIGILLVNFDMIEVTNATDYIGYDPIKGDISPGCSKKHPELC--KLTPANPYQRGCSA 77
          + F+ IL+V    I   +A  YI Y PI+ D+  GC  + P     K TPANPY+R C+ 
Sbjct: 7  VTFVTILIVLCVFISTIHAKRYIEY-PIRLDLGKGCDPRFPTAACYKRTPANPYRRPCTT 66

Query: 78 INRCR 81
           NRCR
Sbjct: 67 ANRCR 70

[Close]

BLAST of Lsi06G005930 vs. TAIR10
Match: AT2G32885.1 (AT2G32885.1 Rapid alkalinization factor (RALF) family protein)

HSP 1 Score: 50.8 bits (120), Expect = 6.0e-07
Identity = 24/66 (36.36%), Postives = 36/66 (54.55%), Query Frame = 1


Query: 17 TLVFIGILLVNFDMIEVTNATDYIGYDPIKGDISPGCSKKHPELCKLTPANPYQRGCSAI 76
          T+V    L++   ++  T   + IG   ++ D+  GC+      CK+ PANPY+ GC A 
Sbjct: 7  TVVQSFALIIIISIVMSTTEANSIGAPAMREDLPKGCAPGSSAGCKMQPANPYKPGCEAS 66

Query: 77 NRCRGG 83
           RCRGG
Sbjct: 67 QRCRGG 72

[Close]

BLAST of Lsi06G005930 vs. TAIR10
Match: AT2G34825.1 (AT2G34825.1 RALF-like 20)

HSP 1 Score: 50.1 bits (118), Expect = 1.0e-06
Identity = 22/58 (37.93%), Postives = 30/58 (51.72%), Query Frame = 1


Query: 24 LLVNFDMIEVTNATDYIGYDPIKGDISPGCSKKHPELCKLTPANPYQRGCSAINRCRG 82
          L++   ++  T     IG   ++ D   GC    P  CK+ PANPY+ GC A  RCRG
Sbjct: 14 LMIILSIVMSTTEAKTIGNPAMREDEPKGCPPGSPASCKMQPANPYKPGCEASQRCRG 71

[Close]

BLAST of Lsi06G005930 vs. NCBI nr
Match: gi|700202865|gb|KGN57998.1| (hypothetical protein Csa_3G426350 [Cucumis sativus])

HSP 1 Score: 117.1 bits (292), Expect = 1.9e-23
Identity = 60/100 (60.00%), Postives = 74/100 (74.00%), Query Frame = 1


Query: 10  ILLGYGITLVFIGILLVNFDMIEVTNATDYIGYDPI-KGDISPGCSKKHPELCKLTPANP 69
           +++   +TLVFIGILLV+FDMI +TNA  +I Y  I  GD+SPGCS  HPELC++  ANP
Sbjct: 1   MIMSKRMTLVFIGILLVSFDMIMITNARKFIDYGSIVAGDVSPGCSPTHPELCRVKSANP 60

Query: 70  YQRGCSAINRCRGGNDIIDGVEE----VASTSPAMSPNAE 105
           YQRGC+ I+RCR GNDIID  EE     AS SP++SPN E
Sbjct: 61  YQRGCNRIDRCREGNDIIDAEEEHIEGDASISPSISPNIE 100

[Close]

BLAST of Lsi06G005930 vs. NCBI nr
Match: gi|18407238|ref|NP_564779.1| (protein RALF-like 9 [Arabidopsis thaliana])

HSP 1 Score: 63.2 bits (152), Expect = 3.3e-07
Identity = 34/67 (50.75%), Postives = 39/67 (58.21%), Query Frame = 1


Query: 19 VFIGILLVNFDMIEVTN--ATDYIGYDPI-KGDISPGCSKKHPELCKLTPANPYQRGCSA 78
          V + + LV F  +  T   AT YI Y  I +GD +  C K HP  CK   ANPYQRGC  
Sbjct: 9  VILSLALVVFLALAATKVEATRYITYPAIDRGDHAVHCDKAHPNTCKKKEANPYQRGCEK 68

Query: 79 INRCRGG 83
          INRCRGG
Sbjct: 69 INRCRGG 75

[Close]

BLAST of Lsi06G005930 vs. NCBI nr
Match: gi|21592626|gb|AAM64575.1| (unknown [Arabidopsis thaliana])

HSP 1 Score: 62.0 bits (149), Expect = 7.4e-07
Identity = 34/67 (50.75%), Postives = 39/67 (58.21%), Query Frame = 1


Query: 19 VFIGILLVNFDMIEVTN--ATDYIGYDPI-KGDISPGCSKKHPELCKLTPANPYQRGCSA 78
          V + + LV F  +  T   AT YI Y  I +GD +  C K HP  CK   ANPYQRGC  
Sbjct: 9  VILSLALVVFLALAGTKVEATRYITYPAIDRGDHAVHCDKAHPNTCKKKQANPYQRGCEK 68

Query: 79 INRCRGG 83
          INRCRGG
Sbjct: 69 INRCRGG 75

The following BLAST results are available for this feature:

BLAST of Lsi06G005930 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RLF9_ARATH	2.1e-09	50.75	Protein RALF-like 9 OS=Arabidopsis thaliana GN=RALFL9 PE=3 SV=1	[more]
RLF15_ARATH	2.8e-06	43.08	Protein RALF-like 15 OS=Arabidopsis thaliana GN=RALFL15 PE=3 SV=1	[more]
RLF29_ARATH	6.2e-06	43.08	Protein RALF-like 29 OS=Arabidopsis thaliana GN=RALFL29 PE=3 SV=1	[more]

BLAST of Lsi06G005930 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L8B1_CUCSA	1.3e-23	60.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G426350 PE=4 SV=1	[more]
D7KVJ4_ARALL	9.7e-06	53.06	Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRA...	[more]

BLAST of Lsi06G005930 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G61566.1	1.2e-10	50.75	ralf-like 9	[more]
AT2G22055.1	1.6e-07	43.08	RALF-like 15	[more]
AT4G11653.1	3.5e-07	43.08	RALF-like 29	[more]
AT2G32885.1	6.0e-07	36.36	Rapid alkalinization factor (RALF) family protein	[more]
AT2G34825.1	1.0e-06	37.93	RALF-like 20	[more]

BLAST of Lsi06G005930 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700202865\|gb\|KGN57998.1\|	1.9e-23	60.00	hypothetical protein Csa_3G426350 [Cucumis sativus]	[more]
gi\|18407238\|ref\|NP_564779.1\|	3.3e-07	50.75	protein RALF-like 9 [Arabidopsis thaliana]	[more]
gi\|21592626\|gb\|AAM64575.1\|	7.4e-07	50.75	unknown [Arabidopsis thaliana]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR008801	RALF

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi06G005930.1	Lsi06G005930.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008801	Rapid ALkalinization Factor	PFAM	PF05498	RALF	coord: 35..81 score: 1.9
None	No IPR available	PANTHER	PTHR34270	FAMILY NOT NAMED	coord: 4..92 score: 2.7
None	No IPR available	PANTHER	PTHR34270:SF1	PROTEIN RALF-LIKE 10-RELATED	coord: 4..92 score: 2.7

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi06G005930	Cucurbita maxima (Rimu)	cmalsiB073
Lsi06G005930	Cucurbita maxima (Rimu)	cmalsiB128
Lsi06G005930	Cucurbita moschata (Rifu)	cmolsiB059
Lsi06G005930	Cucurbita moschata (Rifu)	cmolsiB118
Lsi06G005930	Cucurbita pepo (Zucchini)	cpelsiB299
Lsi06G005930	Cucurbita pepo (Zucchini)	cpelsiB563
Lsi06G005930	Wild cucumber (PI 183967)	cpilsiB059
Lsi06G005930	Cucumber (Chinese Long) v2	culsiB058
Lsi06G005930	Melon (DHL92) v3.5.1	lsimeB396
Lsi06G005930	Watermelon (Charleston Gray)	lsiwcgB364
Lsi06G005930	Watermelon (97103) v1	lsiwmB397
Lsi06G005930	Cucumber (Gy14) v2	cgyblsiB055
Lsi06G005930	Melon (DHL92) v3.6.1	lsimedB418
Lsi06G005930	Silver-seed gourd	carlsiB807
Lsi06G005930	Silver-seed gourd	carlsiB886
Lsi06G005930	Cucumber (Chinese Long) v3	cuclsiB068
Lsi06G005930	Watermelon (97103) v2	lsiwmbB365
Lsi06G005930	Wax gourd	lsiwgoB520
Lsi06G005930	Cucumber (Gy14) v1	cgylsiB573