Lsi06G005570 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi06G005570
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Cytochrome P450
Location	chr06 : 7103593 .. 7107177 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTACATAAAATAATGGAACAGCTCAATACCGAAATTGCATGCATCCGTCTCAGAAAAATCCACGTGATTCTCGTGATGTTTCTAAAGACACACGACTCGATATTCCCATCACAATGTTGAAAATTAAACATGTTTTAATTTGATAAGTTATAGATGAATTAAAGAATGAGAGTTAAGTAAGTATGAATTAAAGAATGGAGTTAAATATGAGTTTCATGTATTTAAGTATAAGAGTGATGGGACCCATGAGAATCCTATAACTAGGATTGTTTTGTATTGTTTAAAGCAACTATGTAGATAAGAATACACAAAGAGAGTTCAATTAAAATGTGTGAACCAAGTTTGAAGTGTCTTTCAAATATAAGTGTTTTGTTACCTCTCAAATATTCTTTTGTTGAATATTTATTTGTGAGTGTCCATCACACGCTTCCAACACGTGACTGATCATCGTCACCACCGACATGTTCAGTGATGGGTTCCTCACGGCAGGTGTGGCACCATGGGAAAATCAATGGAAAAAGATGAGAAGAATACTCAAGTCGAAAATATTTAGTCCGACATGACACTGTTGGATGTTCTCGAAAAGAACAGAAGAAGCTGACAATCTCCTCCGTTACATGTTTAGTCTAACGACGACAACATCGACACTGGTGAAAGTGAGAAGTATAACACAACATTATTCGGGGAATGTGATGAGGAGAATGATGTTCAATAGAAGATACTACGGGAAAGGGAGGGCGGACGAGGGGCCGGCCGCACATGAAGAAGAACATATTGGAGCATTGTTTACAATGCTTCAACATGTTTATGCATTTTGTGTATCAGATTACATGACTTGTTTGAAGGCATTTGGTTTGGATGGCCATGAGAGGACTATGAAGAAGGCATTGAATATTATCCGAATGTTTGAAGAGCCAACTATTGATGAGAGAGTTCAGCAATGGAGAGATGGGAAGAGGAAGGAAGCTGAGGATATGTTGGATATCTTCATTTCACTCGAAGATGGAAATGAGGAGCCATTGTTGTCTGTCAAGGAGATTATAGCACAAATTACTATAAGAATTTGACGGTGCAACTTTCGGGCGTCGTTTAGTTCCCGAAAAGACCTCTAATCAAATGAAATTTATAATCACCAAACTACTACTTATATTACGATGACGTAGCATAAGCGGCAAGTCCGGAGTCGAACTACAGGGAAGCTAGCTCTAATGTATAAGTTTCAAGTTAATTAGTATCTTCTAGCCAAAGTGGTAACGAAGAGTTGGAATGGAATAGAGGAATGCATGTAAGTAAAATAAAGATAACGCAGGCAAATGCTAATTAGTAAATTTCCTATGTAATTTCTATCATGGGATTGGTTCAATTAATAACATATCCGGTCATGGTTTCACAACAACACAACTAACTTAGTCAATTTGATTAATGCTAAGGCAGTCAATTAATCTGATTAAACTAGTCATGCGTCCTAATTATTAATCAAGGTATGAGTCCTAATTAACCTACATGTCTTGCATCTTAACCGAGATCGATAGTGGTCGATTGAGACAAATACATGAGCATTAAGCCTTAGGACTCTCTTATGTTGATTAATTAATAAGATAGCTTGATTAATTAACCAAATTACTTTGAACCTAGTTGAAACATGCATTATAAGATTCAGGATAGCTATAAACCCTAGCATGCACATTTAACTAGTTGCTACTTAGTGATTAGGCTAGATGAACATTAGAGAAACACACAAGCCTAAATTCTTAGCTACGACATACATTCGGGGGTAGGGTTGTCAATCCATTCATAAAATATACATCAAGAGTATAGTTCAAAGACGCACGACCACAATATGAGATAAAATTGGAAGCAATCCTCAAGTTATTACAAAGGTTTACATCCCAAGACAAAGAAAACTACTTACCTCACTTTCATAGACAAAGAAAAGGGAAGAAACTCTAATCCATATTTACAGCCCAAAAATAAAGAAATCTAACCTAAAAATGTGAAAAACTAGGGAAAACCGAGCTGGGGGCTGGTTTTTTCCTCAAATCTCCACACTATTTGCCCTAAAATCGAAGAAGATATTATATAGAAAATAGTGAGAGCGTCGCTATGCTCTCCATGAGAGTAGCGGTGATCGCTTTTCCAGCCGCAGCGTAGCTACGTTGCCTCAATTTCTCGAGTAGCAGCACTTCTGCCTTGGAGCGCAGCGATCCTCCAAGGGCATTTTGGTCCTTTTTCTTCAATTTGTTGCATGATGCTCCAATTCAACCCGAATTGCATTCAAAACGTCCCGATGGTCTCATTTGTTCATTTTCCTATCAAATACTGAAAAAAAAATATTATATTCTAGAGAACTCACCTGATTTAGGCGAAGATAGATAGCTCTTTTCGGGAGCTATCAGAAGTCATCAACTCTTTTAAACTTTTAAACTTCAAGTTTATTCTATTTTCGTTTTAAAATGACACATTTAAGGTTTGAACTTTCTAAAAAACAGTTATTTTAATTTGTGCTATTTGTCTCTTAACTTATTTGACATGAACTGACGTTCATATATAAATGAATTAATTATAATAGTGCTCCTCATGATTGTATTAAAATTAAGATAAAAATAATAATTAAAATAAAGTTTTTCAAAAGATCGAAAACTAAAATAGAATAATTGAAATATCTTAAATAGACATTTTAAAAGTTGAGTGATCAAAATAGAATAAACTTCAAAGTTAGAGACTATGATAGGATTTGAATTATGTTTTTAGATTTACATAATATTTTTGGTTTAGTTCATTTTTTCTTTTCCTTTTCACTTTCGTTGATTTTAGTTTATACTTTTAAAAAATATCATCTCTTTATCCTTATTTGCAAAACTTCAACACAAAGTTTATACACAATGAGAACCATTTAATACAAATGAATACAATTTAAAAAAAAAAATAGTAAATAACGTGTCATGCCTAAAATTTTGATTAAAAAAATAGAAGTGAAGATACCAAAATGCTCACTTTTTAAAAGTACATGTACTAAAATTAACAAAGATTAAAATTGTAAAGATCAAGATGAACTTTTGAAAGCTTTGGGACCTGTTTTTTCTTTTGTTCTTTAGTCTATATATTTTTGGAAAATGACTATTTTGATCTTTATTCCACGTCTCAACACAAAGTTGATACATAAACAAAAAATATTTAATACAAATTCATGATTATTTTGATTAAAGAAAATAAAAATGAAAATAAATATATCAAAATGTTCACATAATATATGCGTCAGGATGAGTTTAGCTCAACTGACATAAATAAAATATATGATAATGATTGAGAAGTTTGTGGTTTTAATGCCTACTTTATTGTAATAATAATTATTAAAAAAAAAAAAAAACCGTAGTTGATCTAATTGGATGTTTTTTCTTTTATAAACAAAATTATAGGAACCGCATGCTTCTTGCAACGGTGGACAATCCATCGAATGTCGTGGAATGGGCCATGGCCGAGATGCTAAACCAACCCCAATTCCTCCAACAAGCCACCGAAGAACTCAACAGAGTCGTCGAAAAACAGAGGCTGGTTCAAGAATCCGATCTCCCCAACCTTAA

mRNA sequence

ATGGAAGAAGCTGACAATCTCCTCCGTTACATGTTTAGTCTAACGACGACAACATCGACACTGGTGAAAGTGAGAAGTATAACACAACATTATTCGGGGAATGTGATGAGGAGAATGATGTTCAATAGAAGATACTACGGGAAAGGGAGGGCGGACGAGGGGCCGGCCGCACATGAAGAAGAACATATTGGAGCATTGTTTACAATGCTTCAACATGTTTATGCATTTTGTGTATCAGATTACATGACTTGTTTGAAGGCATTTGGTTTGGATGGCCATGAGAGGACTATGAAGAAGGCATTGAATATTATCCGAATGTTTGAAGAGCCAACTATTGATGAGAGAGAACCGCATGCTTCTTGCAACGGTGGACAATCCATCGAATGTCGTGGAATGGGCCATGGCCGAGATGCTAAACCAACCCCAATTCCTCCAACAAGCCACCGAAGAACTCAACAGAGTCGTCGAAAAACAGAGGCTGGTTCAAGAATCCGATCTCCCCAACCTTAA

Coding sequence (CDS)

ATGGAAGAAGCTGACAATCTCCTCCGTTACATGTTTAGTCTAACGACGACAACATCGACACTGGTGAAAGTGAGAAGTATAACACAACATTATTCGGGGAATGTGATGAGGAGAATGATGTTCAATAGAAGATACTACGGGAAAGGGAGGGCGGACGAGGGGCCGGCCGCACATGAAGAAGAACATATTGGAGCATTGTTTACAATGCTTCAACATGTTTATGCATTTTGTGTATCAGATTACATGACTTGTTTGAAGGCATTTGGTTTGGATGGCCATGAGAGGACTATGAAGAAGGCATTGAATATTATCCGAATGTTTGAAGAGCCAACTATTGATGAGAGAGAACCGCATGCTTCTTGCAACGGTGGACAATCCATCGAATGTCGTGGAATGGGCCATGGCCGAGATGCTAAACCAACCCCAATTCCTCCAACAAGCCACCGAAGAACTCAACAGAGTCGTCGAAAAACAGAGGCTGGTTCAAGAATCCGATCTCCCCAACCTTAA

Protein sequence

MEEADNLLRYMFSLTTTTSTLVKVRSITQHYSGNVMRRMMFNRRYYGKGRADEGPAAHEEEHIGALFTMLQHVYAFCVSDYMTCLKAFGLDGHERTMKKALNIIRMFEEPTIDEREPHASCNGGQSIECRGMGHGRDAKPTPIPPTSHRRTQQSRRKTEAGSRIRSPQP

Homology

[Close]

BLAST of Lsi06G005570 vs. Swiss-Prot
Match: C79A1_SORBI (Tyrosine N-monooxygenase OS=Sorghum bicolor GN=CYP79A1 PE=1 SV=3)

HSP 1 Score: 115.2 bits (287), Expect = 7.2e-25
Identity = 56/117 (47.86%), Postives = 79/117 (67.52%), Query Frame = 1


Query: 2   EEADNLLRYMFSLTTTTST---LVKVRSITQHYSGNVMRRMMFNRRYYGKGRADEGPAAH 61
           +EADNL RY+++L T  +T    V VR + +HY GNV+RR+MFNRRY+G+ +AD GP   
Sbjct: 189 DEADNLTRYVYNLATKAATGDVAVDVRHVARHYCGNVIRRLMFNRRYFGEPQADGGPGPM 248

Query: 62  EEEHIGALFTMLQHVYAFCVSDYMTCLKAFGLDGHERTMKKALNIIRMFEEPTIDER 116
           E  H+ A+FT L  +YAFCVSDY+  L+   LDGHE+ +K+A   +    +  ID+R
Sbjct: 249 EVLHMDAVFTSLGLLYAFCVSDYLPWLRGLDLDGHEKIVKEANVAVNRLHDTVIDDR 305

[Close]

BLAST of Lsi06G005570 vs. Swiss-Prot
Match: C79D3_LOTJA (Isoleucine N-monooxygenase 1 OS=Lotus japonicus GN=CYP79D3 PE=1 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 3.9e-23
Identity = 53/118 (44.92%), Postives = 75/118 (63.56%), Query Frame = 1


Query: 2   EEADNLLRYMFSLTTTTST----LVKVRSITQHYSGNVMRRMMFNRRYYGKGRADEGPAA 61
           EEADNL+ Y+++           LV +R   QHY GNV R+++FN RY+GK   D GP  
Sbjct: 166 EEADNLMFYIYNKCCKDVNDGPGLVNIRIAAQHYGGNVFRKLIFNSRYFGKVMEDGGPGF 225

Query: 62  HEEEHIGALFTMLQHVYAFCVSDYMTCLKAFGLDGHERTMKKALNIIRMFEEPTIDER 116
            E EHI A FT+L++VYAF +SD++  L+   LDGH   + KA+ I+R + +P ID+R
Sbjct: 226 EEVEHINATFTILKYVYAFSISDFVPFLRRLDLDGHRSKIMKAMRIMRKYHDPIIDDR 283

[Close]

BLAST of Lsi06G005570 vs. Swiss-Prot
Match: C79D1_MANES (Valine N-monooxygenase 1 OS=Manihot esculenta GN=CYP79D1 PE=1 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 1.1e-22
Identity = 52/114 (45.61%), Postives = 75/114 (65.79%), Query Frame = 1


Query: 2   EEADNLLRYMFSLTTTTSTLVKVRSITQHYSGNVMRRMMFNRRYYGKGRADEGPAAHEEE 61
           EEADNL+ Y+ +        V +R+ T+HY GNV+R+M+F++RY+GKG  D GP   E E
Sbjct: 180 EEADNLVFYIHNQFKANKN-VNLRTATRHYGGNVIRKMVFSKRYFGKGMPDGGPGPEEIE 239

Query: 62  HIGALFTMLQHVYAFCVSDYMTCLKAFGLDGHERTMKKALNIIRMFEEPTIDER 116
           HI A+FT L+++Y FC+SD++  L    LDG E+ +  A   IR ++ P IDER
Sbjct: 240 HIDAVFTALKYLYGFCISDFLPFLLGLDLDGQEKFVLDANKTIRDYQNPLIDER 292

[Close]

BLAST of Lsi06G005570 vs. Swiss-Prot
Match: C79D4_LOTJA (Isoleucine N-monooxygenase 2 OS=Lotus japonicus GN=CYP79D4 PE=1 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 5.7e-22
Identity = 51/118 (43.22%), Postives = 74/118 (62.71%), Query Frame = 1


Query: 2   EEADNLLRYMFSLTTTTST----LVKVRSITQHYSGNVMRRMMFNRRYYGKGRADEGPAA 61
           EEADNL+ Y+++           LV +R   QHY GNV R+++FN RY+GK   D GP  
Sbjct: 166 EEADNLMFYIYNKCCKDVNDGPGLVNIRIAAQHYGGNVFRKLIFNTRYFGKVMEDGGPGF 225

Query: 62  HEEEHIGALFTMLQHVYAFCVSDYMTCLKAFGLDGHERTMKKALNIIRMFEEPTIDER 116
            E EHI A FT+L++VYAF +SD++  L+   LDGH   + KA+ I++ + +P I +R
Sbjct: 226 EEVEHINATFTILKYVYAFSISDFIPFLRRLDLDGHRSKIMKAMGIMKKYHDPIIHDR 283

[Close]

BLAST of Lsi06G005570 vs. Swiss-Prot
Match: C79D2_MANES (Valine N-monooxygenase 2 OS=Manihot esculenta GN=CYP79D2 PE=1 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 3.7e-21
Identity = 50/114 (43.86%), Postives = 72/114 (63.16%), Query Frame = 1


Query: 2   EEADNLLRYMFSLTTTTSTLVKVRSITQHYSGNVMRRMMFNRRYYGKGRADEGPAAHEEE 61
           EEAD L+ Y+ +   +    V VR   +HY GNV+R+MMF++RY+GKG  D GP   E  
Sbjct: 179 EEADQLVFYINNQYKSNKN-VNVRIAARHYGGNVIRKMMFSKRYFGKGMPDGGPGPEEIM 238

Query: 62  HIGALFTMLQHVYAFCVSDYMTCLKAFGLDGHERTMKKALNIIRMFEEPTIDER 116
           H+ A+FT L+++Y FC+SDY+  L+   LDG E+ +  A   IR  + P I+ER
Sbjct: 239 HVDAIFTALKYLYGFCISDYLPFLEGLDLDGQEKIVLNANKTIRDLQNPLIEER 291

[Close]

BLAST of Lsi06G005570 vs. TrEMBL
Match: A0A0A0LXQ7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G617390 PE=4 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 1.1e-48
Identity = 97/114 (85.09%), Postives = 102/114 (89.47%), Query Frame = 1


Query: 2   EEADNLLRYMFSLTTTTSTLVKVRSITQHYSGNVMRRMMFNRRYYGKGRADEGPAAHEEE 61
           EEADNLLRY+FSLT TT T V VRSITQHYSGNVMRRMMFNRRYYGKGR D GPA  EEE
Sbjct: 169 EEADNLLRYVFSLTKTTPTSVNVRSITQHYSGNVMRRMMFNRRYYGKGRPDGGPATEEEE 228

Query: 62  HIGALFTMLQHVYAFCVSDYMTCLKAFGLDGHERTMKKALNIIRMFEEPTIDER 116
           HIGALFTMLQHVYAFCVSDYM+CLKAF LDGHER +KKALN+IR +EEP IDER
Sbjct: 229 HIGALFTMLQHVYAFCVSDYMSCLKAFDLDGHERIVKKALNVIRKYEEPIIDER 282

[Close]

BLAST of Lsi06G005570 vs. TrEMBL
Match: A0A0A0LXR0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G620390 PE=4 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 1.3e-33
Identity = 72/115 (62.61%), Postives = 88/115 (76.52%), Query Frame = 1


Query: 3   EADNLLRYMFSLTTTT--STLVKVRSITQHYSGNVMRRMMFNRRYYGKGRADEGPAAHEE 62
           EAD LLRY+FS+T+    S ++ VRSITQHY GN++R M+FNRRYYGKGR D GP   EE
Sbjct: 180 EADTLLRYIFSITSGNGGSAVINVRSITQHYCGNIIRTMLFNRRYYGKGREDGGPTLEEE 239

Query: 63  EHIGALFTMLQHVYAFCVSDYMTCLKAFGLDGHERTMKKALNIIRMFEEPTIDER 116
           EH  AL T+L+HV AF +SD+M CLK F LDGHE+ MK ALN++R  +EP IDER
Sbjct: 240 EHNQALLTILRHVNAFSISDFMPCLKPFDLDGHEKIMKNALNVVRKHDEPIIDER 294

[Close]

BLAST of Lsi06G005570 vs. TrEMBL
Match: A0A0B0MTD4_GOSAR (Tyrosine N-monooxygenase OS=Gossypium arboreum GN=F383_30003 PE=4 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 2.6e-29
Identity = 61/115 (53.04%), Postives = 87/115 (75.65%), Query Frame = 1


Query: 1   MEEADNLLRYMFSLTTTTSTLVKVRSITQHYSGNVMRRMMFNRRYYGKGRADEGPAAHEE 60
           +EEADNL+RY+++L T+   LV VR  +Q Y GNV R+M+FNRRY+G+G+ D GP   EE
Sbjct: 181 VEEADNLVRYVYNLCTSAGGLVNVRVASQQYCGNVPRKMLFNRRYFGEGKEDGGPGFEEE 240

Query: 61  EHIGALFTMLQHVYAFCVSDYMTCLKAFGLDGHERTMKKALNIIRMFEEPTIDER 116
           EHI A+FT+L ++Y+FCVSDY+  L+   LDGHE+ M  AL++++ + +P IDER
Sbjct: 241 EHISAVFTILAYLYSFCVSDYLPRLRGLDLDGHEKVMAGALSVMQKYHDPIIDER 295

[Close]

BLAST of Lsi06G005570 vs. TrEMBL
Match: A0A0D2THV7_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_009G113900 PE=4 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 5.7e-29
Identity = 61/115 (53.04%), Postives = 86/115 (74.78%), Query Frame = 1


Query: 1   MEEADNLLRYMFSLTTTTSTLVKVRSITQHYSGNVMRRMMFNRRYYGKGRADEGPAAHEE 60
           +EEADNL+RY+++L T+   LV VR  +Q Y GNV R+M+FNRRY+G+G+ D GP   EE
Sbjct: 181 VEEADNLVRYVYNLCTSAGGLVNVRVASQQYCGNVPRKMLFNRRYFGEGKEDGGPGFEEE 240

Query: 61  EHIGALFTMLQHVYAFCVSDYMTCLKAFGLDGHERTMKKALNIIRMFEEPTIDER 116
           EHI A+FT+L ++Y+FCVSDY+  L+   LDGHE+ M  AL +++ + +P IDER
Sbjct: 241 EHISAVFTILAYLYSFCVSDYLPRLRGLDLDGHEKVMAGALAVMQKYHDPIIDER 295

[Close]

BLAST of Lsi06G005570 vs. TrEMBL
Match: A0A059B871_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_H04899 PE=3 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 4.8e-28
Identity = 60/114 (52.63%), Postives = 81/114 (71.05%), Query Frame = 1


Query: 2   EEADNLLRYMFSLTTTTSTLVKVRSITQHYSGNVMRRMMFNRRYYGKGRADEGPAAHEEE 61
           EEADNL++Y+F+L  T+   V +R+ T+HY GNV RR+MFN+RY+GKGR D GP   EEE
Sbjct: 163 EEADNLVKYVFNLCKTSGHQVNLRATTRHYCGNVARRLMFNKRYFGKGRQDGGPTIDEEE 222

Query: 62  HIGALFTMLQHVYAFCVSDYMTCLKAFGLDGHERTMKKALNIIRMFEEPTIDER 116
           H+ A+F  L ++YAFCVSDY   L    LDGHE+ +K+++  +R   EP I ER
Sbjct: 223 HVEAIFNALYYLYAFCVSDYFPFLVGLDLDGHEKKVKESVRTLRRLHEPIISER 276

[Close]

BLAST of Lsi06G005570 vs. TAIR10
Match: AT5G35917.1 (AT5G35917.1 cytochrome P450, family 79, subfamily A, polypeptide 3 pseudogene)

HSP 1 Score: 102.1 bits (253), Expect = 3.5e-22
Identity = 50/121 (41.32%), Postives = 77/121 (63.64%), Query Frame = 1


Query: 2   EEADNLLRYMFSLTTTTS------TLVKVRSITQHYSGNVMRRMMFNRRYYGKGRADE-G 61
           EEADNL+RY+ +             ++ +R + + YSGNV R+MMF  R++GKG  DE G
Sbjct: 164 EEADNLVRYINNRCVKNRGNGNGLAVIDLRFVVRQYSGNVARKMMFGIRHFGKGSEDESG 223

Query: 62  PAAHEEEHIGALFTMLQHVYAFCVSDYMTCLKAFGLDGHERTMKKALNIIRMFEEPTIDE 116
           P + E EH+ +LFT+L H+YAF +SDY+  L+   L+GHE+ +  A+  +  + +P +DE
Sbjct: 224 PGSEEIEHVESLFTVLTHLYAFALSDYVPWLRFLDLEGHEKVVADAMRNVSKYNDPFVDE 283

[Close]

BLAST of Lsi06G005570 vs. TAIR10
Match: AT5G05260.1 (AT5G05260.1 cytochrome p450 79a2)

HSP 1 Score: 100.1 bits (248), Expect = 1.3e-21
Identity = 49/119 (41.18%), Postives = 75/119 (63.03%), Query Frame = 1


Query: 2   EEADNLLRYMFSLTTTTS----TLVKVRSITQHYSGNVMRRMMFNRRYYGKGRAD-EGPA 61
           EEADNL+RY+ + +         ++ +R   + YSGNV R+MMF  R++GKG  D  GP 
Sbjct: 156 EEADNLVRYINNRSVKNRGNAFVVIDLRLAVRQYSGNVARKMMFGIRHFGKGSEDGSGPG 215

Query: 62  AHEEEHIGALFTMLQHVYAFCVSDYMTCLKAFGLDGHERTMKKALNIIRMFEEPTIDER 116
             E EH+ +LFT+L H+YAF +SDY+  L+   L+GHE+ +  A+  +  + +P +DER
Sbjct: 216 LEEIEHVESLFTVLTHLYAFALSDYVPWLRFLDLEGHEKVVSNAMRNVSKYNDPFVDER 274

[Close]

BLAST of Lsi06G005570 vs. TAIR10
Match: AT2G22330.1 (AT2G22330.1 cytochrome P450, family 79, subfamily B, polypeptide 3)

HSP 1 Score: 89.7 bits (221), Expect = 1.8e-18
Identity = 43/115 (37.39%), Postives = 71/115 (61.74%), Query Frame = 1


Query: 2   EEADNLLRYMFSLTTTTSTLVKVRSITQHYSGNVMRRMMFNRRYYG-KGRADEGPAAHEE 61
           EE D+L  +++++   +   V +R +T+HY GN ++R+MF  R +  K  AD GP   + 
Sbjct: 179 EETDHLTAWLYNMVKNSEP-VDLRFVTRHYCGNAIKRLMFGTRTFSEKTEADGGPTLEDI 238

Query: 62  EHIGALFTMLQHVYAFCVSDYMTCLKAFGLDGHERTMKKALNIIRMFEEPTIDER 116
           EH+ A+F  L   +AFC+SDY+  L    L+GHE+ M+++  I+  + +P IDER
Sbjct: 239 EHMDAMFEGLGFTFAFCISDYLPMLTGLDLNGHEKIMRESSAIMDKYHDPIIDER 292

[Close]

BLAST of Lsi06G005570 vs. TAIR10
Match: AT4G39950.1 (AT4G39950.1 cytochrome P450, family 79, subfamily B, polypeptide 2)

HSP 1 Score: 88.2 bits (217), Expect = 5.3e-18
Identity = 42/115 (36.52%), Postives = 71/115 (61.74%), Query Frame = 1


Query: 2   EEADNLLRYMFSLTTTTSTLVKVRSITQHYSGNVMRRMMFNRRYYGKGRA-DEGPAAHEE 61
           EE D+L  +++++   + + V  R +T+HY GN ++++MF  R + K  A D GP   + 
Sbjct: 177 EENDHLTAWVYNMVKNSGS-VDFRFMTRHYCGNAIKKLMFGTRTFSKNTAPDGGPTVEDV 236

Query: 62  EHIGALFTMLQHVYAFCVSDYMTCLKAFGLDGHERTMKKALNIIRMFEEPTIDER 116
           EH+ A+F  L   +AFC+SDY+  L    L+GHE+ M+++  I+  + +P IDER
Sbjct: 237 EHMEAMFEALGFTFAFCISDYLPMLTGLDLNGHEKIMRESSAIMDKYHDPIIDER 290

[Close]

BLAST of Lsi06G005570 vs. NCBI nr
Match: gi|659099192|ref|XP_008450479.1| (PREDICTED: tyrosine N-monooxygenase-like [Cucumis melo])

HSP 1 Score: 201.4 bits (511), Expect = 1.2e-48
Identity = 97/114 (85.09%), Postives = 103/114 (90.35%), Query Frame = 1


Query: 2   EEADNLLRYMFSLTTTTSTLVKVRSITQHYSGNVMRRMMFNRRYYGKGRADEGPAAHEEE 61
           EEADNLLRY+FSLT TT T V VRSITQHYSGNVMRRMMFNRRY+GKGRAD GPA  EEE
Sbjct: 146 EEADNLLRYVFSLTKTTPTSVSVRSITQHYSGNVMRRMMFNRRYFGKGRADGGPATEEEE 205

Query: 62  HIGALFTMLQHVYAFCVSDYMTCLKAFGLDGHERTMKKALNIIRMFEEPTIDER 116
           HIGALFTMLQHVYAFCVSDYM+CLKAF LDGHER +KKALN+IR +EEP IDER
Sbjct: 206 HIGALFTMLQHVYAFCVSDYMSCLKAFDLDGHERIVKKALNVIRKYEEPIIDER 259

[Close]

BLAST of Lsi06G005570 vs. NCBI nr
Match: gi|449442705|ref|XP_004139121.1| (PREDICTED: tyrosine N-monooxygenase-like [Cucumis sativus])

HSP 1 Score: 201.1 bits (510), Expect = 1.6e-48
Identity = 97/114 (85.09%), Postives = 102/114 (89.47%), Query Frame = 1


Query: 2   EEADNLLRYMFSLTTTTSTLVKVRSITQHYSGNVMRRMMFNRRYYGKGRADEGPAAHEEE 61
           EEADNLLRY+FSLT TT T V VRSITQHYSGNVMRRMMFNRRYYGKGR D GPA  EEE
Sbjct: 169 EEADNLLRYVFSLTKTTPTSVNVRSITQHYSGNVMRRMMFNRRYYGKGRPDGGPATEEEE 228

Query: 62  HIGALFTMLQHVYAFCVSDYMTCLKAFGLDGHERTMKKALNIIRMFEEPTIDER 116
           HIGALFTMLQHVYAFCVSDYM+CLKAF LDGHER +KKALN+IR +EEP IDER
Sbjct: 229 HIGALFTMLQHVYAFCVSDYMSCLKAFDLDGHERIVKKALNVIRKYEEPIIDER 282

[Close]

BLAST of Lsi06G005570 vs. NCBI nr
Match: gi|700211427|gb|KGN66523.1| (hypothetical protein Csa_1G617390 [Cucumis sativus])

HSP 1 Score: 201.1 bits (510), Expect = 1.6e-48
Identity = 97/114 (85.09%), Postives = 102/114 (89.47%), Query Frame = 1


Query: 2   EEADNLLRYMFSLTTTTSTLVKVRSITQHYSGNVMRRMMFNRRYYGKGRADEGPAAHEEE 61
           EEADNLLRY+FSLT TT T V VRSITQHYSGNVMRRMMFNRRYYGKGR D GPA  EEE
Sbjct: 169 EEADNLLRYVFSLTKTTPTSVNVRSITQHYSGNVMRRMMFNRRYYGKGRPDGGPATEEEE 228

Query: 62  HIGALFTMLQHVYAFCVSDYMTCLKAFGLDGHERTMKKALNIIRMFEEPTIDER 116
           HIGALFTMLQHVYAFCVSDYM+CLKAF LDGHER +KKALN+IR +EEP IDER
Sbjct: 229 HIGALFTMLQHVYAFCVSDYMSCLKAFDLDGHERIVKKALNVIRKYEEPIIDER 282

[Close]

BLAST of Lsi06G005570 vs. NCBI nr
Match: gi|778663781|ref|XP_004151661.2| (PREDICTED: isoleucine N-monooxygenase 1-like [Cucumis sativus])

HSP 1 Score: 151.0 bits (380), Expect = 1.9e-33
Identity = 72/115 (62.61%), Postives = 88/115 (76.52%), Query Frame = 1


Query: 3   EADNLLRYMFSLTTTT--STLVKVRSITQHYSGNVMRRMMFNRRYYGKGRADEGPAAHEE 62
           EAD LLRY+FS+T+    S ++ VRSITQHY GN++R M+FNRRYYGKGR D GP   EE
Sbjct: 180 EADTLLRYIFSITSGNGGSAVINVRSITQHYCGNIIRTMLFNRRYYGKGREDGGPTLEEE 239

Query: 63  EHIGALFTMLQHVYAFCVSDYMTCLKAFGLDGHERTMKKALNIIRMFEEPTIDER 116
           EH  AL T+L+HV AF +SD+M CLK F LDGHE+ MK ALN++R  +EP IDER
Sbjct: 240 EHNQALLTILRHVNAFSISDFMPCLKPFDLDGHEKIMKNALNVVRKHDEPIIDER 294

[Close]

BLAST of Lsi06G005570 vs. NCBI nr
Match: gi|700211432|gb|KGN66528.1| (hypothetical protein Csa_1G620390 [Cucumis sativus])

HSP 1 Score: 151.0 bits (380), Expect = 1.9e-33
Identity = 72/115 (62.61%), Postives = 88/115 (76.52%), Query Frame = 1


Query: 3   EADNLLRYMFSLTTTT--STLVKVRSITQHYSGNVMRRMMFNRRYYGKGRADEGPAAHEE 62
           EAD LLRY+FS+T+    S ++ VRSITQHY GN++R M+FNRRYYGKGR D GP   EE
Sbjct: 180 EADTLLRYIFSITSGNGGSAVINVRSITQHYCGNIIRTMLFNRRYYGKGREDGGPTLEEE 239

Query: 63  EHIGALFTMLQHVYAFCVSDYMTCLKAFGLDGHERTMKKALNIIRMFEEPTIDER 116
           EH  AL T+L+HV AF +SD+M CLK F LDGHE+ MK ALN++R  +EP IDER
Sbjct: 240 EHNQALLTILRHVNAFSISDFMPCLKPFDLDGHEKIMKNALNVVRKHDEPIIDER 294

The following BLAST results are available for this feature:

BLAST of Lsi06G005570 vs. Swiss-Prot


Match Name	E-value	Identity	Description
C79A1_SORBI	7.2e-25	47.86	Tyrosine N-monooxygenase OS=Sorghum bicolor GN=CYP79A1 PE=1 SV=3	[more]
C79D3_LOTJA	3.9e-23	44.92	Isoleucine N-monooxygenase 1 OS=Lotus japonicus GN=CYP79D3 PE=1 SV=1	[more]
C79D1_MANES	1.1e-22	45.61	Valine N-monooxygenase 1 OS=Manihot esculenta GN=CYP79D1 PE=1 SV=1	[more]
C79D4_LOTJA	5.7e-22	43.22	Isoleucine N-monooxygenase 2 OS=Lotus japonicus GN=CYP79D4 PE=1 SV=1	[more]
C79D2_MANES	3.7e-21	43.86	Valine N-monooxygenase 2 OS=Manihot esculenta GN=CYP79D2 PE=1 SV=1	[more]

BLAST of Lsi06G005570 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LXQ7_CUCSA	1.1e-48	85.09	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G617390 PE=4 SV=1	[more]
A0A0A0LXR0_CUCSA	1.3e-33	62.61	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G620390 PE=4 SV=1	[more]
A0A0B0MTD4_GOSAR	2.6e-29	53.04	Tyrosine N-monooxygenase OS=Gossypium arboreum GN=F383_30003 PE=4 SV=1	[more]
A0A0D2THV7_GOSRA	5.7e-29	53.04	Uncharacterized protein OS=Gossypium raimondii GN=B456_009G113900 PE=4 SV=1	[more]
A0A059B871_EUCGR	4.8e-28	52.63	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_H04899 PE=3 SV=1	[more]

BLAST of Lsi06G005570 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G35917.1	3.5e-22	41.32	cytochrome P450, family 79, subfamily A, polypeptide 3 pseudogene	[more]
AT5G05260.1	1.3e-21	41.18	cytochrome p450 79a2	[more]
AT2G22330.1	1.8e-18	37.39	cytochrome P450, family 79, subfamily B, polypeptide 3	[more]
AT4G39950.1	5.3e-18	36.52	cytochrome P450, family 79, subfamily B, polypeptide 2	[more]

BLAST of Lsi06G005570 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659099192\|ref\|XP_008450479.1\|	1.2e-48	85.09	PREDICTED: tyrosine N-monooxygenase-like [Cucumis melo]	[more]
gi\|449442705\|ref\|XP_004139121.1\|	1.6e-48	85.09	PREDICTED: tyrosine N-monooxygenase-like [Cucumis sativus]	[more]
gi\|700211427\|gb\|KGN66523.1\|	1.6e-48	85.09	hypothetical protein Csa_1G617390 [Cucumis sativus]	[more]
gi\|778663781\|ref\|XP_004151661.2\|	1.9e-33	62.61	PREDICTED: isoleucine N-monooxygenase 1-like [Cucumis sativus]	[more]
gi\|700211432\|gb\|KGN66528.1\|	1.9e-33	62.61	hypothetical protein Csa_1G620390 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0020037	heme binding
GO:0005506	iron ion binding
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Vocabulary: **INTERPRO**
Term	Definition
IPR001128	Cyt_P450

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
molecular_function	GO:0004497	monooxygenase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi06G005570.1	Lsi06G005570.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	GENE3D	G3DSA:1.10.630.10		coord: 2..117 score: 2.
IPR001128	Cytochrome P450	unknown	SSF48264	Cytochrome P450	coord: 2..118 score: 4.5
None	No IPR available	PANTHER	PTHR24298	FAMILY NOT NAMED	coord: 2..115 score: 1.3
None	No IPR available	PANTHER	PTHR24298:SF1	CYTOCHROME P450, FAMILY 79, SUBFAMILY C, POLYPEPTIDE 1-RELATED	coord: 2..115 score: 1.3

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi06G005570	MELO3C015148.2	Melon (DHL92) v3.6.1	lsimedB418
Lsi06G005570	Bhi02G001267	Wax gourd	lsiwgoB520
Lsi06G005570	CsGy1G030030	Cucumber (Gy14) v2	cgyblsiB055
Lsi06G005570	CsaV3_1G043190	Cucumber (Chinese Long) v3	cuclsiB068

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi06G005570	Bottle gourd (USVL1VR-Ls)	lsilsiB046
Lsi06G005570	Cucumber (Gy14) v1	cgylsiB044
Lsi06G005570	Cucurbita maxima (Rimu)	cmalsiB073
Lsi06G005570	Cucurbita maxima (Rimu)	cmalsiB128
Lsi06G005570	Cucurbita maxima (Rimu)	cmalsiB527
Lsi06G005570	Cucurbita moschata (Rifu)	cmolsiB059
Lsi06G005570	Cucurbita moschata (Rifu)	cmolsiB118
Lsi06G005570	Cucurbita moschata (Rifu)	cmolsiB512
Lsi06G005570	Cucurbita pepo (Zucchini)	cpelsiB246
Lsi06G005570	Cucurbita pepo (Zucchini)	cpelsiB299
Lsi06G005570	Cucurbita pepo (Zucchini)	cpelsiB563
Lsi06G005570	Wild cucumber (PI 183967)	cpilsiB059
Lsi06G005570	Wild cucumber (PI 183967)	cpilsiB475
Lsi06G005570	Cucumber (Chinese Long) v2	culsiB058
Lsi06G005570	Cucumber (Chinese Long) v2	culsiB467
Lsi06G005570	Melon (DHL92) v3.5.1	lsimeB396
Lsi06G005570	Melon (DHL92) v3.5.1	lsimeB415
Lsi06G005570	Watermelon (Charleston Gray)	lsiwcgB360
Lsi06G005570	Watermelon (Charleston Gray)	lsiwcgB364
Lsi06G005570	Watermelon (97103) v1	lsiwmB396
Lsi06G005570	Watermelon (97103) v1	lsiwmB410
Lsi06G005570	Melon (DHL92) v3.6.1	lsimedB439
Lsi06G005570	Silver-seed gourd	carlsiB385
Lsi06G005570	Silver-seed gourd	carlsiB477
Lsi06G005570	Watermelon (97103) v2	lsiwmbB361
Lsi06G005570	Watermelon (97103) v2	lsiwmbB365
Lsi06G005570	Wax gourd	lsiwgoB487