BLAST of Lsi06G004510 vs. Swiss-Prot
Match:
ASO_CUCSA (L-ascorbate oxidase OS=Cucumis sativus PE=1 SV=1)
HSP 1 Score: 973.4 bits (2515), Expect = 1.1e-282
Identity = 456/591 (77.16%), Postives = 513/591 (86.80%), Query Frame = 1
Query: 1 MAKLANQPLFSMPFLVIFLLFSIISGFGITLSEA-VSQTRHYKWEIENMFWSPDCTENLV 60
MAK+A++P F PFL FL+ SII GFGITLSEA + +HYKW++E MFWSPDC EN+V
Sbjct: 1 MAKVADKPFFPKPFLS-FLVLSIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIV 60
Query: 61 MGINGQFPGPTIRANVGDTIIVEMINKLTTESVVIHWHGILQKETPWADGTASISQCATN 120
MGING+FPGPTIRAN GD ++VE+ NKL TE VVIHWHGILQ+ TPWADGTASISQCA N
Sbjct: 61 MGINGEFPGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAIN 120
Query: 121 PGETYLYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFQYDEDINLLLSDW 180
PGET+ YRFVVDKAGTYFYHGHLGMQR+AGLYGSLIVD EG+ EPF YDE+INLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDW 180
Query: 181 WHKSVYKQEAGLSSSPMRWIGEPESILINGRGQTNCSIAAKYKKGVEQCKLKGSEECAPF 240
WH+SV+KQE GLSS PMRWIGEP+SILING+GQ +CSIAAKY +G++QC+L G E+CAPF
Sbjct: 181 WHQSVHKQEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPF 240
Query: 241 ILKVKPNKTYRIRIASTTSLSALNFAIGNHQLLLVEADGNYVQPFLTSDIDIYSGESYSV 300
IL V+P KTYRIRIASTT+L++LNFAIGNH+LL+VEADGNYVQPF+TSDIDIYSGESYSV
Sbjct: 241 ILHVQPKKTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSV 300
Query: 301 LITTDQTPTENYWVSIGVRGRKPKTPPALTLLNYLPNSVSKLPISPPPETPQWDDYDHSK 360
LITTDQ P ENYWVSIGVR R PKTPP LTLLNYLPNS SKLPISPPPETP W+D+D SK
Sbjct: 301 LITTDQNPLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRSK 360
Query: 361 NFTYKILATMGSPKPPVKSNRRIFLLNTQNNMSGGFTKWAINNVSLALPPTPYLAAIRIG 420
NFT++I A MGSPKPPV+ NRR+FLLNTQN ++ GF KWAINNVSLALPPTPYLAA+++
Sbjct: 361 NFTFRIFAAMGSPKPPVRYNRRLFLLNTQNRIN-GFMKWAINNVSLALPPTPYLAAMKMR 420
Query: 421 LNDTFDQNPPPETFPEDYDIYT-PPSTSTRTGNGIYRFELGEIVDVILQNANTLEKDRSE 480
LN F+QNPPPETFP +YDI PP+ T TGNG+Y+F +GE VDVILQNAN L + SE
Sbjct: 421 LNTAFNQNPPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSE 480
Query: 481 IHPWHLHGHDFWVLGYGDGKFSSDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
IHPWHLHGHDFWVLGYG+GKF + DEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
Query: 541 WPFHCHIEPHLHMGMGVVFAEGVHKVKRIPTKALACGETAKVLINNPTKKP 590
W FHCHIEPHLHMGMGVVFAEGVH V IP KALACG TA L+ N + P
Sbjct: 541 WAFHCHIEPHLHMGMGVVFAEGVHMVGMIPPKALACGSTA--LVKNYPRLP 587
BLAST of Lsi06G004510 vs. Swiss-Prot
Match:
ASO_CUCMA (L-ascorbate oxidase OS=Cucurbita maxima GN=AAO PE=1 SV=2)
HSP 1 Score: 955.7 bits (2469), Expect = 2.4e-277
Identity = 445/573 (77.66%), Postives = 496/573 (86.56%), Query Frame = 1
Query: 14 FLVIFLLFSIISGFGITLSEAVSQTRHYKWEIENMFWSPDCTENLVMGINGQFPGPTIRA 73
FL++F F +I FGI S SQ RHYKWE+E MFW+PDC EN+VMGINGQFPGPTIRA
Sbjct: 12 FLILFF-FGLILAFGI--SSEGSQIRHYKWEVEYMFWAPDCNENIVMGINGQFPGPTIRA 71
Query: 74 NVGDTIIVEMINKLTTESVVIHWHGILQKETPWADGTASISQCATNPGETYLYRFVVDKA 133
N GDT++VE+INKL TE VVIHWHGILQ+ TPWADGTASISQCA NPGET+ Y F VD
Sbjct: 72 NAGDTVVVELINKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFFYNFTVDNP 131
Query: 134 GTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFQYDEDINLLLSDWWHKSVYKQEAGLSS 193
GT+FYHGHLGMQR+AGLYGSLIVD +GKKEPF YD +INLLLSDWWH+S++KQE GLSS
Sbjct: 132 GTFFYHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLSS 191
Query: 194 SPMRWIGEPESILINGRGQTNCSIAAKYKKGVEQCKLKGSEECAPFILKVKPNKTYRIRI 253
P+RWIGEP++IL+NGRGQ +CSIAAKY +E CKLKGSE CAP+I V P KTYRIRI
Sbjct: 192 KPIRWIGEPQTILLNGRGQFDCSIAAKYDSNLEPCKLKGSEPCAPYIFHVMPKKTYRIRI 251
Query: 254 ASTTSLSALNFAIGNHQLLLVEADGNYVQPFLTSDIDIYSGESYSVLITTDQTPTENYWV 313
ASTT+L+ALNFAIGNH LL+VEADGNYVQPF TSDIDIYSGESYSVLITTDQ P+ENYWV
Sbjct: 252 ASTTALAALNFAIGNHPLLVVEADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWV 311
Query: 314 SIGVRGRKPKTPPALTLLNYLPNSVSKLPISPPPETPQWDDYDHSKNFTYKILATMGSPK 373
S+G RGR P TPP LTLLNYLPNSVSKLP SPPPETP WDD+D SKNFTY+I A MGSPK
Sbjct: 312 SVGTRGRHPNTPPGLTLLNYLPNSVSKLPTSPPPETPAWDDFDRSKNFTYRITAAMGSPK 371
Query: 374 PPVKSNRRIFLLNTQNNMSGGFTKWAINNVSLALPPTPYLAAIRIGLNDTFDQNPPPETF 433
PPVKSNRRIFLLNTQ N+ G+ KWAIN+VSLALPPTPYL A++ L FDQNPPPE F
Sbjct: 372 PPVKSNRRIFLLNTQ-NVINGYVKWAINDVSLALPPTPYLGAMKFNLLHAFDQNPPPEVF 431
Query: 434 PEDYDIYTPPST-STRTGNGIYRFELGEIVDVILQNANTLEKDRSEIHPWHLHGHDFWVL 493
PEDYDI TPP+ T+ GNG+Y+F++GEIVDVILQNAN ++++ SEIHPWHLHGHDFWVL
Sbjct: 432 PEDYDIDTPPTNEKTKIGNGVYQFKIGEIVDVILQNANMMKENLSEIHPWHLHGHDFWVL 491
Query: 494 GYGDGKFSSDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPFHCHIEPHLHMG 553
GYGDGKF+++ +E LNLKNPPLRNTVVIFPYGWTAIRFVADNPGVW FHCHIEPHLHMG
Sbjct: 492 GYGDGKFTAE-EESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMG 551
Query: 554 MGVVFAEGVHKVKRIPTKALACGETAKVLINNP 586
MGVVFAEGV KV RIPTKALACG TAK LINNP
Sbjct: 552 MGVVFAEGVEKVGRIPTKALACGGTAKSLINNP 579
BLAST of Lsi06G004510 vs. Swiss-Prot
Match:
ASO_CUCPM (L-ascorbate oxidase OS=Cucurbita pepo var. melopepo PE=1 SV=1)
HSP 1 Score: 941.0 bits (2431), Expect = 6.1e-273
Identity = 431/551 (78.22%), Postives = 481/551 (87.30%), Query Frame = 1
Query: 36 SQTRHYKWEIENMFWSPDCTENLVMGINGQFPGPTIRANVGDTIIVEMINKLTTESVVIH 95
SQ RHYKWE+E MFW+P+C EN+VMGINGQFPGPTIRAN GD+++VE+ NKL TE VVIH
Sbjct: 1 SQIRHYKWEVEYMFWAPNCNENIVMGINGQFPGPTIRANAGDSVVVELTNKLHTEGVVIH 60
Query: 96 WHGILQKETPWADGTASISQCATNPGETYLYRFVVDKAGTYFYHGHLGMQRAAGLYGSLI 155
WHGILQ+ TPWADGTASISQCA NPGET+ Y F VD GT+FYHGHLGMQR+AGLYGSLI
Sbjct: 61 WHGILQRGTPWADGTASISQCAINPGETFFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLI 120
Query: 156 VDLEEGKKEPFQYDEDINLLLSDWWHKSVYKQEAGLSSSPMRWIGEPESILINGRGQTNC 215
VD +GKKEPF YD +INLLLSDWWH+S++KQE GLSS P+RWIGEP++IL+NGRGQ +C
Sbjct: 121 VDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLSSKPIRWIGEPQTILLNGRGQFDC 180
Query: 216 SIAAKYKKGVEQCKLKGSEECAPFILKVKPNKTYRIRIASTTSLSALNFAIGNHQLLLVE 275
SIAAKY +E CKLKGSE CAP+I V P KTYRIRIASTT+L+ALNFAIGNHQLL+VE
Sbjct: 181 SIAAKYDSNLEPCKLKGSESCAPYIFHVSPKKTYRIRIASTTALAALNFAIGNHQLLVVE 240
Query: 276 ADGNYVQPFLTSDIDIYSGESYSVLITTDQTPTENYWVSIGVRGRKPKTPPALTLLNYLP 335
ADGNYVQPF TSDIDIYSGESYSVLITTDQ P+ENYWVS+G R R P TPP LTLLNYLP
Sbjct: 241 ADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWVSVGTRARHPNTPPGLTLLNYLP 300
Query: 336 NSVSKLPISPPPETPQWDDYDHSKNFTYKILATMGSPKPPVKSNRRIFLLNTQNNMSGGF 395
NSVSKLP SPPP+TP WDD+D SKNFTY+I A MGSPKPPVK NRRIFLLNTQ N+ G+
Sbjct: 301 NSVSKLPTSPPPQTPAWDDFDRSKNFTYRITAAMGSPKPPVKFNRRIFLLNTQ-NVINGY 360
Query: 396 TKWAINNVSLALPPTPYLAAIRIGLNDTFDQNPPPETFPEDYDIYTPPST-STRTGNGIY 455
KWAIN+VSLALPPTPYL A++ L FDQNPPPE FPEDYDI TPP+ TR GNG+Y
Sbjct: 361 VKWAINDVSLALPPTPYLGAMKYNLLHAFDQNPPPEVFPEDYDIDTPPTNEKTRIGNGVY 420
Query: 456 RFELGEIVDVILQNANTLEKDRSEIHPWHLHGHDFWVLGYGDGKFSSDVDEKKLNLKNPP 515
+F++GE+VDVILQNAN ++++ SE HPWHLHGHDFWVLGYGDGKFS++ +E LNLKNPP
Sbjct: 421 QFKIGEVVDVILQNANMMKENLSETHPWHLHGHDFWVLGYGDGKFSAE-EESSLNLKNPP 480
Query: 516 LRNTVVIFPYGWTAIRFVADNPGVWPFHCHIEPHLHMGMGVVFAEGVHKVKRIPTKALAC 575
LRNTVVIFPYGWTAIRFVADNPGVW FHCHIEPHLHMGMGVVFAEGV KV RIPTKALAC
Sbjct: 481 LRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVEKVGRIPTKALAC 540
Query: 576 GETAKVLINNP 586
G TAK LINNP
Sbjct: 541 GGTAKSLINNP 549
BLAST of Lsi06G004510 vs. Swiss-Prot
Match:
ASO_TOBAC (L-ascorbate oxidase OS=Nicotiana tabacum GN=AAO PE=2 SV=1)
HSP 1 Score: 830.1 bits (2143), Expect = 1.5e-239
Identity = 383/576 (66.49%), Postives = 464/576 (80.56%), Query Frame = 1
Query: 11 SMPFLVIFLLFSIISGFGITLSEAVSQTRHYKWEIENMFWSPDCTENLVMGINGQFPGPT 70
S+ FL FLL I+ + S ++TRH+KW++E + WSPD E++VMGINGQFPGPT
Sbjct: 3 SLGFLFFFLLPLILLELSSSRSVMAAKTRHFKWDVEYIHWSPDGEESVVMGINGQFPGPT 62
Query: 71 IRANVGDTIIVEMINKLTTESVVIHWHGILQKETPWADGTASISQCATNPGETYLYRFVV 130
IRA GDT+ V + NKL TE VVIHWHGI Q TPWADGTA+ISQCA NPGET+LYRF V
Sbjct: 63 IRAKAGDTVAVHLTNKLHTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGETFLYRFKV 122
Query: 131 DKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFQYDEDINLLLSDWWHKSVYKQEAG 190
DKAGTYFYHGH GMQR+AGLYGSLIV++ EG+KEPF YD + NLLLSDWWHK ++QE
Sbjct: 123 DKAGTYFYHGHYGMQRSAGLYGSLIVEVGEGEKEPFHYDGEFNLLLSDWWHKGSHEQEVD 182
Query: 191 LSSSPMRWIGEPESILINGRGQTNCSIAAKYKK-GVEQCKLKGSEECAPFILKVKPNKTY 250
LSS+P+RWIGEP+++L+NGRGQ NCS+AA++ K + QCKL+G E+ AP IL+V+PNK Y
Sbjct: 183 LSSNPLRWIGEPQTLLLNGRGQYNCSLAARFSKPPLPQCKLRGGEQYAPQILRVRPNKIY 242
Query: 251 RIRIASTTSLSALNFAIGNHQLLLVEADGNYVQPFLTSDIDIYSGESYSVLITTDQTPTE 310
R+R+ASTT+L +L+ AIG H++++VEADGNYVQPF D+DIYSGESYSVL TDQ PT+
Sbjct: 243 RLRVASTTALGSLSLAIGGHKMVVVEADGNYVQPFSVQDMDIYSGESYSVLFKTDQDPTK 302
Query: 311 NYWVSIGVRGRKPKTPPALTLLNYLPNSVSKLPISPPPETPQWDDYDHSKNFTYKILATM 370
NYW+SI VRGR+PKTP LTLLNYLPNS SK P PPP P W+DY+HSK+F+ KI A M
Sbjct: 303 NYWISINVRGREPKTPQGLTLLNYLPNSASKFPTLPPPIAPLWNDYNHSKSFSNKIFALM 362
Query: 371 GSPKPPVKSNRRIFLLNTQNNMSGGFTKWAINNVSLALPPTPYLAAIRIGLNDTFDQNPP 430
GSPKPP +++RRI LLNTQN + G+TKWAINNVSL LP YL +IR G+N FD PP
Sbjct: 363 GSPKPPPQNHRRIILLNTQNKID-GYTKWAINNVSLVLPTQLYLGSIRYGIN-AFDTKPP 422
Query: 431 PETFPEDYDIY-TPPSTSTRTGNGIYRFELGEIVDVILQNANTLEKDRSEIHPWHLHGHD 490
P+ FP+DYD+ P++++ GNG+Y + +D+ILQNAN L KD SEIHPWHLHGHD
Sbjct: 423 PDNFPKDYDVLKQAPNSNSTYGNGVYMLKFNTTIDIILQNANALAKDVSEIHPWHLHGHD 482
Query: 491 FWVLGYGDGKFSSDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPFHCHIEPH 550
FWVLGYG+GKF S+ D KK NLKNPPLRNT VIFP+GWTA+RFV DNPGVW FHCHIEPH
Sbjct: 483 FWVLGYGEGKF-SEKDVKKFNLKNPPLRNTAVIFPFGWTALRFVTDNPGVWAFHCHIEPH 542
Query: 551 LHMGMGVVFAEGVHKVKRIPTKALACGETAKVLINN 585
LHMGMGV+FAEGVH VK+IP +ALACG T K+L++N
Sbjct: 543 LHMGMGVIFAEGVHLVKKIPKEALACGLTGKMLMSN 575
BLAST of Lsi06G004510 vs. Swiss-Prot
Match:
LAC11_ARATH (Laccase-11 OS=Arabidopsis thaliana GN=LAC11 PE=2 SV=1)
HSP 1 Score: 266.2 bits (679), Expect = 8.8e-70
Identity = 177/565 (31.33%), Postives = 281/565 (49.73%), Query Frame = 1
Query: 12 MPFLVIFLLFSIISGFGITLSEAVSQTRHYKWEIENMFWSPDCTENLVMGINGQFPGPTI 71
M FL +F G+ S + + Y+++++ S C ++ +NG FPGPT+
Sbjct: 3 MGFLFLFCYLLAFLGY----SPVDAAVKKYQFDVQVKNISRICNAKPIVTVNGMFPGPTV 62
Query: 72 RANVGDTIIVEMINKLTTESVVIHWHGILQKETPWADGTASISQCATNPGETYLYRF-VV 131
A GD +I+ + N + ++ IHWHG+ Q WADG A I+QC G++YLY F V
Sbjct: 63 YAREGDRVIINVTNHVQY-NMSIHWHGLKQYRNGWADGPAYITQCPIQTGQSYLYDFNVT 122
Query: 132 DKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPF-QYDEDINLLLSDWWHKSVYKQEA 191
+ GT ++H H+ RA +YG++++ GK PF Q ++ N++L +WW+K V E
Sbjct: 123 GQRGTLWWHAHILWLRAT-VYGAIVILPAPGKPYPFPQPYQESNIILGEWWNKDV---ET 182
Query: 192 GLSSSPMRWIGEP--ESILINGRGQTNCSIAAKYKKGVEQCKLKGSEECAPFILKVKPNK 251
++ + P ++ ING+ + K+ F+++ + K
Sbjct: 183 AVNQANQLGAPPPMSDAHTINGKPGPLFPCSEKH----------------TFVIEAEAGK 242
Query: 252 TYRIRIASTTSLSALNFAIGNHQLLLVEADGNYVQPFLTSDIDIYSGESYSVLITTDQTP 311
TY +RI + L F I H + +VE D Y +PF T I + G++ +VL+ TD++P
Sbjct: 243 TYLLRIINAALNDELFFGIAGHNMTVVEIDAVYTKPFTTKAILLGPGQTTNVLVKTDRSP 302
Query: 312 TENYWVSIGVRGRKP-----KTPPALTLLNYLPNSVSKLPISPPPETPQWDDYDHSKNFT 371
Y+++ P KT A+ +PN+V LPI P P + N
Sbjct: 303 -NRYFMAASPFMDAPVSVDNKTVTAILQYKGVPNTV--LPILPKLPLPNDTSFALDYNGK 362
Query: 372 YKILATMGSPK-PPVKSNRRIF------LLNTQNNMSGGFTKWAINNVSLALPPTPYLAA 431
K L T P P+K +RR+F + ++G +INN++ +P T L A
Sbjct: 363 LKSLNTPNFPALVPLKVDRRLFYTIGLGINACPTCVNGTNLAASINNITFIMPKTALLKA 422
Query: 432 IRIGLNDTFDQNPPPETFPEDYDIYTPPSTS---TRTGNGIYRFELGEIVDVILQNANTL 491
++ F + P+ P+ ++ P T+ T TG + R + ++++LQ+ N L
Sbjct: 423 HYSNISGVF-RTDFPDRPPKAFNYTGVPLTANLGTSTGTRLSRVKFNTTIELVLQDTNLL 482
Query: 492 EKDRSEIHPWHLHGHDFWVLGYGDGKFSSDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFV 551
E HP+HLHG++F+V+G G G F D K NL +PP RNTV + GW AIRF
Sbjct: 483 ---TVESHPFHLHGYNFFVVGTGVGNFDPKKDPAKFNLVDPPERNTVGVPTGGWAAIRFR 535
Query: 552 ADNPGVWPFHCHIEPHLHMGMGVVF 558
ADNPGVW HCH+E H G+ + F
Sbjct: 543 ADNPGVWFMHCHLEVHTMWGLKMAF 535
BLAST of Lsi06G004510 vs. TrEMBL
Match:
Q9M5B5_CUCME (Ascorbate oxidase AO1 OS=Cucumis melo PE=2 SV=1)
HSP 1 Score: 1054.3 bits (2725), Expect = 5.5e-305
Identity = 490/588 (83.33%), Postives = 535/588 (90.99%), Query Frame = 1
Query: 3 KLANQPLFSMPFLVIFLLFSIISGFGITLSEAVSQTRHYKWEIENMFWSPDCTENLVMGI 62
K+ANQPLF MPF IF +FSI GFGITLS+A SQTRH+KWE+E MF SPDC ENLVMGI
Sbjct: 4 KVANQPLFPMPFPAIFFIFSIFFGFGITLSQAASQTRHFKWEVEYMFRSPDCKENLVMGI 63
Query: 63 NGQFPGPTIRANVGDTIIVEMINKLTTESVVIHWHGILQKETPWADGTASISQCATNPGE 122
N QFPGPTIRANVGDT++VE+INKL+TE VVIHWHGILQK TPWADGTASISQCA NPGE
Sbjct: 64 NHQFPGPTIRANVGDTVVVELINKLSTEDVVIHWHGILQKGTPWADGTASISQCAINPGE 123
Query: 123 TYLYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFQYDEDINLLLSDWWHK 182
++ Y+FVVDK GTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPF YDE+INLLLSDWWH+
Sbjct: 124 SFTYQFVVDKPGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEEINLLLSDWWHE 183
Query: 183 SVYKQEAGLSSSPMRWIGEPESILINGRGQTNCSIAAKYKKGVEQCKLKGSEECAPFILK 242
SV KQE GLSSSPMRWIGEP+SILINGRGQ+NCSI AKY K +EQCKL GSEECAPFILK
Sbjct: 184 SVLKQEVGLSSSPMRWIGEPQSILINGRGQSNCSIGAKYMKNMEQCKLSGSEECAPFILK 243
Query: 243 VKPNKTYRIRIASTTSLSALNFAIGNHQLLLVEADGNYVQPFLTSDIDIYSGESYSVLIT 302
VKPNKTYRIRI STTSLSALNFAIGNH+LL+VEADGNYVQPFLTSDIDIYSGESYSVLI
Sbjct: 244 VKPNKTYRIRIISTTSLSALNFAIGNHKLLVVEADGNYVQPFLTSDIDIYSGESYSVLIN 303
Query: 303 TDQTPTENYWVSIGVRGRKPKTPPALTLLNYLPNSVSKLPISPPPETPQWDDYDHSKNFT 362
TDQ P++NYWVS+GVRGR+PKTPP LTLLNYLPNS SKLPISPPPETP+W+DYD SKNFT
Sbjct: 304 TDQNPSQNYWVSLGVRGREPKTPPGLTLLNYLPNSFSKLPISPPPETPKWNDYDRSKNFT 363
Query: 363 YKILATMGSPKPPVKSNRRIFLLNTQNNMSGGFTKWAINNVSLALPPTPYLAAIRIGLND 422
Y+ILA MGSPKPPVKS RRIFLLNTQN M+GG+TKWAINNVSL LP TPYL AI+IG+ND
Sbjct: 364 YRILAAMGSPKPPVKSTRRIFLLNTQNKMNGGYTKWAINNVSLTLPSTPYLGAIKIGIND 423
Query: 423 TFDQNPPPETFPEDYDIY-TPPSTSTRTGNGIYRFELGEIVDVILQNANTLEKDRSEIHP 482
TFDQNPPPET+PE+YDI+ PP+ +T TGNG+YRF+LGEIVDV+LQNANTL SEIHP
Sbjct: 424 TFDQNPPPETYPENYDIHKPPPNGNTITGNGVYRFQLGEIVDVVLQNANTLNDKHSEIHP 483
Query: 483 WHLHGHDFWVLGYGDGKFSSDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPF 542
WHLHGHDFWVLGYG+GKFS+ VDE KLNLK+PPLRNTVVIFP+GWT +RFVADNPGVW F
Sbjct: 484 WHLHGHDFWVLGYGEGKFSAAVDETKLNLKDPPLRNTVVIFPFGWTVLRFVADNPGVWAF 543
Query: 543 HCHIEPHLHMGMGVVFAEGVHKVKRIPTKALACGETAKVLINNPTKKP 590
HCHIEPHLHMGMGVVFAEGV KV RIPT A+ACG TAK LI NPT+KP
Sbjct: 544 HCHIEPHLHMGMGVVFAEGVDKVGRIPTHAMACGGTAKALIQNPTQKP 591
BLAST of Lsi06G004510 vs. TrEMBL
Match:
Q9M5B4_CUCME (Ascorbate oxidase AO4 OS=Cucumis melo PE=2 SV=1)
HSP 1 Score: 978.0 bits (2527), Expect = 5.0e-282
Identity = 459/591 (77.66%), Postives = 513/591 (86.80%), Query Frame = 1
Query: 1 MAKLANQPLFSMPFLVIFLLFSIISGFGITLSEA-VSQTRHYKWEIENMFWSPDCTENLV 60
MAK+A++P F PFLV F+L SII GFGITLSEA + RHYKWE+E MFWSPDC EN+V
Sbjct: 1 MAKVADKPFFPKPFLVFFVL-SIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIV 60
Query: 61 MGINGQFPGPTIRANVGDTIIVEMINKLTTESVVIHWHGILQKETPWADGTASISQCATN 120
MGINGQFPGPTIRAN GD ++VE+ NKL TE VVIHWHGILQ+ TPWADGTASISQCA N
Sbjct: 61 MGINGQFPGPTIRANAGDMVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAIN 120
Query: 121 PGETYLYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFQYDEDINLLLSDW 180
PGET+ YRFVVDKAGTYFYHGHLGMQR+AGLYGSLIVD +EG EPF YDE+INLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPQEGTSEPFHYDEEINLLLSDW 180
Query: 181 WHKSVYKQEAGLSSSPMRWIGEPESILINGRGQTNCSIAAKYKKGVEQCKLKGSEECAPF 240
WH+SV+KQE GLSS PMRWIGEP+SILINGRGQ +CSIAAKYK G++QC+L G E+CAPF
Sbjct: 181 WHQSVHKQEVGLSSKPMRWIGEPQSILINGRGQFDCSIAAKYKNGLKQCELSGKEQCAPF 240
Query: 241 ILKVKPNKTYRIRIASTTSLSALNFAIGNHQLLLVEADGNYVQPFLTSDIDIYSGESYSV 300
IL V P K YRIRIASTT+L++LNFAIGNH+LL+VEADGNYVQPF TSDIDIYSGESYSV
Sbjct: 241 ILHVHPKKIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFSTSDIDIYSGESYSV 300
Query: 301 LITTDQTPTENYWVSIGVRGRKPKTPPALTLLNYLPNSVSKLPISPPPETPQWDDYDHSK 360
LITTDQ P+ENYWVSIGVR R PKTPP LT+LNYLPNSVSKLPISPPPETP W+D+D SK
Sbjct: 301 LITTDQNPSENYWVSIGVRARLPKTPPGLTVLNYLPNSVSKLPISPPPETPDWEDFDRSK 360
Query: 361 NFTYKILATMGSPKPPVKSNRRIFLLNTQNNMSGGFTKWAINNVSLALPPTPYLAAIRIG 420
NFT++I A MGSPKPPV+ NRR+FLLNTQN ++ GF KWAINNVSLALPPTPYLAA++
Sbjct: 361 NFTFRIFAAMGSPKPPVRYNRRLFLLNTQNRIN-GFMKWAINNVSLALPPTPYLAAMKFR 420
Query: 421 LNDTFDQNPPPETFPEDYDIYTPP-STSTRTGNGIYRFELGEIVDVILQNANTLEKDRSE 480
L+ F+QNPPPETFP DYDI PP + T TGNG+Y+F +GE VDVILQNAN L + SE
Sbjct: 421 LSTAFNQNPPPETFPIDYDINNPPRNPETATGNGVYKFNMGETVDVILQNANMLNANTSE 480
Query: 481 IHPWHLHGHDFWVLGYGDGKFSSDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
IHPWHLHGHDFWVLGYG+GKFS+ D +KLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFSAAEDGRKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
Query: 541 WPFHCHIEPHLHMGMGVVFAEGVHKVKRIPTKALACGETAKVLINNPTKKP 590
W FHCHIEPHLHMGMGV+FAEGVHKV IP A+ACG TA +I N +KP
Sbjct: 541 WAFHCHIEPHLHMGMGVIFAEGVHKVGMIPPNAMACGSTA--MIKNYPRKP 587
BLAST of Lsi06G004510 vs. TrEMBL
Match:
A0A0A0LXB9_CUCSA (L-ascorbate oxidase OS=Cucumis sativus GN=Csa_1G604040 PE=3 SV=1)
HSP 1 Score: 973.4 bits (2515), Expect = 1.2e-280
Identity = 456/591 (77.16%), Postives = 513/591 (86.80%), Query Frame = 1
Query: 1 MAKLANQPLFSMPFLVIFLLFSIISGFGITLSEA-VSQTRHYKWEIENMFWSPDCTENLV 60
MAK+A++P F PFL FL+ SII GFGITLSEA + +HYKW++E MFWSPDC EN+V
Sbjct: 1 MAKVADKPFFPKPFLS-FLVLSIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIV 60
Query: 61 MGINGQFPGPTIRANVGDTIIVEMINKLTTESVVIHWHGILQKETPWADGTASISQCATN 120
MGING+FPGPTIRAN GD ++VE+ NKL TE VVIHWHGILQ+ TPWADGTASISQCA N
Sbjct: 61 MGINGEFPGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAIN 120
Query: 121 PGETYLYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFQYDEDINLLLSDW 180
PGET+ YRFVVDKAGTYFYHGHLGMQR+AGLYGSLIVD EG+ EPF YDE+INLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDW 180
Query: 181 WHKSVYKQEAGLSSSPMRWIGEPESILINGRGQTNCSIAAKYKKGVEQCKLKGSEECAPF 240
WH+SV+KQE GLSS PMRWIGEP+SILING+GQ +CSIAAKY +G++QC+L G E+CAPF
Sbjct: 181 WHQSVHKQEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPF 240
Query: 241 ILKVKPNKTYRIRIASTTSLSALNFAIGNHQLLLVEADGNYVQPFLTSDIDIYSGESYSV 300
IL V+P KTYRIRIASTT+L++LNFAIGNH+LL+VEADGNYVQPF+TSDIDIYSGESYSV
Sbjct: 241 ILHVQPKKTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSV 300
Query: 301 LITTDQTPTENYWVSIGVRGRKPKTPPALTLLNYLPNSVSKLPISPPPETPQWDDYDHSK 360
LITTDQ P ENYWVSIGVR R PKTPP LTLLNYLPNS SKLPISPPPETP W+D+D SK
Sbjct: 301 LITTDQNPLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRSK 360
Query: 361 NFTYKILATMGSPKPPVKSNRRIFLLNTQNNMSGGFTKWAINNVSLALPPTPYLAAIRIG 420
NFT++I A MGSPKPPV+ NRR+FLLNTQN ++ GF KWAINNVSLALPPTPYLAA+++
Sbjct: 361 NFTFRIFAAMGSPKPPVRYNRRLFLLNTQNRIN-GFMKWAINNVSLALPPTPYLAAMKMR 420
Query: 421 LNDTFDQNPPPETFPEDYDIYT-PPSTSTRTGNGIYRFELGEIVDVILQNANTLEKDRSE 480
LN F+QNPPPETFP +YDI PP+ T TGNG+Y+F +GE VDVILQNAN L + SE
Sbjct: 421 LNTAFNQNPPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSE 480
Query: 481 IHPWHLHGHDFWVLGYGDGKFSSDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
IHPWHLHGHDFWVLGYG+GKF + DEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
Query: 541 WPFHCHIEPHLHMGMGVVFAEGVHKVKRIPTKALACGETAKVLINNPTKKP 590
W FHCHIEPHLHMGMGVVFAEGVH V IP KALACG TA L+ N + P
Sbjct: 541 WAFHCHIEPHLHMGMGVVFAEGVHMVGMIPPKALACGSTA--LVKNYPRLP 587
BLAST of Lsi06G004510 vs. TrEMBL
Match:
E7BBM8_CUCSA (Ascorbate oxidase OS=Cucumis sativus GN=aso PE=2 SV=1)
HSP 1 Score: 971.5 bits (2510), Expect = 4.7e-280
Identity = 455/591 (76.99%), Postives = 513/591 (86.80%), Query Frame = 1
Query: 1 MAKLANQPLFSMPFLVIFLLFSIISGFGITLSEA-VSQTRHYKWEIENMFWSPDCTENLV 60
MAK+A++P F PFL FL+ SII GFGITLSEA + +HYKW++E MFWSPDC EN+V
Sbjct: 1 MAKVADKPFFPKPFLS-FLVLSIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIV 60
Query: 61 MGINGQFPGPTIRANVGDTIIVEMINKLTTESVVIHWHGILQKETPWADGTASISQCATN 120
MGING+FPGPTIRAN GD ++VE+ NKL TE VVIHWHGILQ+ TPWADGTASISQCA N
Sbjct: 61 MGINGEFPGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAIN 120
Query: 121 PGETYLYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFQYDEDINLLLSDW 180
PGET+ YRFVVDKAGTYFYHGHLGMQR+AGLYGSLIVD EG+ EPF YDE+INLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDW 180
Query: 181 WHKSVYKQEAGLSSSPMRWIGEPESILINGRGQTNCSIAAKYKKGVEQCKLKGSEECAPF 240
WH+SV+KQE GLSS PMRWIGEP+SILING+GQ +CSIAAKY +G++QC+L G E+CAPF
Sbjct: 181 WHQSVHKQEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPF 240
Query: 241 ILKVKPNKTYRIRIASTTSLSALNFAIGNHQLLLVEADGNYVQPFLTSDIDIYSGESYSV 300
IL V+P KTYRIRIASTT+L++LNFAIGNH+LL+VEADGNYVQPF+TSDIDIYSGESYSV
Sbjct: 241 ILHVQPKKTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSV 300
Query: 301 LITTDQTPTENYWVSIGVRGRKPKTPPALTLLNYLPNSVSKLPISPPPETPQWDDYDHSK 360
LITTDQ P ENYWVSIGVR R PKTPP LTLLNYLPNS SKLPISPPPETP W+D+D SK
Sbjct: 301 LITTDQNPLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRSK 360
Query: 361 NFTYKILATMGSPKPPVKSNRRIFLLNTQNNMSGGFTKWAINNVSLALPPTPYLAAIRIG 420
NFT++I A MGSPKPPV+ NRR+FLLNTQN ++ GF KWAINNVSLALPPTPYLAA+++
Sbjct: 361 NFTFRIFAAMGSPKPPVRYNRRLFLLNTQNRIN-GFMKWAINNVSLALPPTPYLAAMKMR 420
Query: 421 LNDTFDQNPPPETFPEDYDIYT-PPSTSTRTGNGIYRFELGEIVDVILQNANTLEKDRSE 480
LN F+Q+PPPETFP +YDI PP+ T TGNG+Y+F +GE VDVILQNAN L + SE
Sbjct: 421 LNTAFNQSPPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSE 480
Query: 481 IHPWHLHGHDFWVLGYGDGKFSSDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
IHPWHLHGHDFWVLGYG+GKF + DEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
Query: 541 WPFHCHIEPHLHMGMGVVFAEGVHKVKRIPTKALACGETAKVLINNPTKKP 590
W FHCHIEPHLHMGMGVVFAEGVH V IP KALACG TA L+ N + P
Sbjct: 541 WAFHCHIEPHLHMGMGVVFAEGVHMVGMIPPKALACGSTA--LVKNYPRLP 587
BLAST of Lsi06G004510 vs. TrEMBL
Match:
A0A0A0LZW2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G602540 PE=4 SV=1)
HSP 1 Score: 950.7 bits (2456), Expect = 8.6e-274
Identity = 444/539 (82.37%), Postives = 487/539 (90.35%), Query Frame = 1
Query: 3 KLANQPLFSMPFLVIFLLFSIISGFGITLSEAVSQTRHYKWEIENMFWSPDCTENLVMGI 62
K+ANQPLF MPF +F L SII GFGITLS+AVSQTRHY WE+E MFWSPDC ENLVMGI
Sbjct: 4 KVANQPLFPMPFPAMFFLLSIIFGFGITLSDAVSQTRHYTWEVEYMFWSPDCKENLVMGI 63
Query: 63 NGQFPGPTIRANVGDTIIVEMINKLTTESVVIHWHGILQKETPWADGTASISQCATNPGE 122
NGQFPGPTIRANVGDT++VEMINKL+TE VVIHWHGILQK TPWADGTASISQCATNPGE
Sbjct: 64 NGQFPGPTIRANVGDTVVVEMINKLSTEDVVIHWHGILQKGTPWADGTASISQCATNPGE 123
Query: 123 TYLYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFQYDEDINLLLSDWWHK 182
++ Y+FVVDK GTYFYHGHLGMQRAAGLYGSLIVD EEG+KEPF YDE+INLLLSDWWH+
Sbjct: 124 SFTYQFVVDKPGTYFYHGHLGMQRAAGLYGSLIVDPEEGEKEPFHYDEEINLLLSDWWHE 183
Query: 183 SVYKQEAGLSSSPMRWIGEPESILINGRGQTNCSIAAKY--KKGVEQCKLKGSEECAPFI 242
S+ KQE GLSSSPMRWIGEP+SILINGRGQ+NCSIAAKY K +EQCKL GSE+CAPFI
Sbjct: 184 SILKQEIGLSSSPMRWIGEPQSILINGRGQSNCSIAAKYMNMKKMEQCKLSGSEQCAPFI 243
Query: 243 LKVKPNKTYRIRIASTTSLSALNFAIGNHQLLLVEADGNYVQPFLTSDIDIYSGESYSVL 302
L+VKPNK YRIRI STTSLSALNFAIG H+LL+VEADGNYVQPFLTSDIDIYSGESYSVL
Sbjct: 244 LEVKPNKIYRIRITSTTSLSALNFAIGKHKLLVVEADGNYVQPFLTSDIDIYSGESYSVL 303
Query: 303 ITTDQTPTENYWVSIGVRGRKPKTPPALTLLNYLPNSVSKLPISPPPETPQWDDYDHSKN 362
I TDQ P+ENYWVS+G+RGR PKTPP LT+LNYLPN VSKLP SPPPETP+W+DYD SKN
Sbjct: 304 INTDQKPSENYWVSLGIRGRDPKTPPGLTILNYLPNPVSKLPTSPPPETPKWNDYDRSKN 363
Query: 363 FTYKILATMGSPKPPVKSNRRIFLLNTQNNMSGGFTKWAINNVSLALPPTPYLAAIRIGL 422
FT++ILA MGSPKPP+KS RRIFLLNTQN M+GG+TKWAINNVSLALP TPYL AI+IGL
Sbjct: 364 FTFQILAAMGSPKPPLKSTRRIFLLNTQNKMNGGYTKWAINNVSLALPSTPYLGAIKIGL 423
Query: 423 NDTFDQNPPPETFPEDYDI-YTPPSTSTRTGNGIYRFELGEIVDVILQNANTLEKDRSEI 482
N TFDQNPPPET+PEDYDI PP+ +T TGNG+YRF++GEIVDV+LQNANTL SEI
Sbjct: 424 NHTFDQNPPPETYPEDYDINKPPPNRNTITGNGVYRFQIGEIVDVVLQNANTLNDKHSEI 483
Query: 483 HPWHLHGHDFWVLGYGDGKFSSDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 539
HPWHL GHDFWVLGYG+GKFSS DE KLNLKNPPLRNTVVIFP+GWT +RFVADNPGV
Sbjct: 484 HPWHLPGHDFWVLGYGEGKFSSAADETKLNLKNPPLRNTVVIFPFGWTVLRFVADNPGV 542
BLAST of Lsi06G004510 vs. TAIR10
Match:
AT5G21105.1 (AT5G21105.1 Plant L-ascorbate oxidase)
HSP 1 Score: 772.7 bits (1994), Expect = 1.6e-223
Identity = 348/553 (62.93%), Postives = 429/553 (77.58%), Query Frame = 1
Query: 34 AVSQTRHYKWEIENMFWSPDCTENLVMGINGQFPGPTIRANVGDTIIVEMINKLTTESVV 93
A + R Y WE+E +WSPDC E VM +NG+FPGPTI+A GDTI+V + NKLTTE +V
Sbjct: 33 ASAAVREYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAGDTIVVNLTNKLTTEGLV 92
Query: 94 IHWHGILQKETPWADGTASISQCATNPGETYLYRFVVDKAGTYFYHGHLGMQRAAGLYGS 153
IHWHGI Q +PWADG A ++QCA NPGET+ Y F V+K GT+FYHGH GMQR+AGLYGS
Sbjct: 93 IHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTHFYHGHYGMQRSAGLYGS 152
Query: 154 LIVDLEEGKKEPFQYDEDINLLLSDWWHKSVYKQEAGLSSSPMRWIGEPESILINGRGQT 213
LIVD+ +GK E +YD + NLLLSDWWH+++ QE GLSS PMRWIGE +SILINGRGQ
Sbjct: 153 LIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSSKPMRWIGEAQSILINGRGQF 212
Query: 214 NCSIAAKYKK--GVEQCKLKGSEECAPFILKVKPNKTYRIRIASTTSLSALNFAIGNHQL 273
NCS+AA++ + C K ++CAP IL V+PNKTYRIR++STT+L++LN A+ H+L
Sbjct: 213 NCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALASLNLAVQGHKL 272
Query: 274 LLVEADGNYVQPFLTSDIDIYSGESYSVLITTDQTPTENYWVSIGVRGRKPKTPPALTLL 333
++VEADGNY+ PF T DIDIYSGESYSVL+TTDQ P++NY++S+GVRGRKP T ALT+L
Sbjct: 273 VVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGRKPNTTQALTIL 332
Query: 334 NYLPNSVSKLPISPPPETPQWDDYDHSKNFTYKILATMGSPKPPVKSNRRIFLLNTQNNM 393
NY+ SKLP SPPP TP+WDD++ SKNF+ KI + MGSP PP K +R+ LLNTQ N+
Sbjct: 333 NYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSPPKKYRKRLILLNTQ-NL 392
Query: 394 SGGFTKWAINNVSLALPPTPYLAAIRIGLNDTFDQNPPPETFPEDYDIYTPPS-TSTRTG 453
G+TKWAINNVSL P TPYL +++ L F++ PP ++ DYDI PP +T TG
Sbjct: 393 IDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMDYDIMNPPPFPNTTTG 452
Query: 454 NGIYRFELGEIVDVILQNANTLEKDRSEIHPWHLHGHDFWVLGYGDGKFSSDVDEKKLNL 513
NGIY F VDVI+QNAN L+ SEIHPWHLHGHDFWVLGYGDGKF +DEK NL
Sbjct: 453 NGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKFKPGIDEKTYNL 512
Query: 514 KNPPLRNTVVIFPYGWTAIRFVADNPGVWPFHCHIEPHLHMGMGVVFAEGVHKVKRIPTK 573
KNPPLRNT +++PYGWTAIRFV DNPGVW FHCHIEPHLHMGMGVVFAEG++++ ++P +
Sbjct: 513 KNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEGLNRIGKVPDE 572
Query: 574 ALACGETAKVLIN 584
AL CG T + L+N
Sbjct: 573 ALGCGLTKQFLMN 584
BLAST of Lsi06G004510 vs. TAIR10
Match:
AT5G21100.1 (AT5G21100.1 Plant L-ascorbate oxidase)
HSP 1 Score: 729.9 bits (1883), Expect = 1.2e-210
Identity = 344/578 (59.52%), Postives = 435/578 (75.26%), Query Frame = 1
Query: 12 MPFLVIFLLFSIISGFGITLSEAVSQTRHYKWEIENMFWSPDCTENLVMGINGQFPGPTI 71
M +V +LL ++ F + V T WE+E +W PDC E +VM INGQFPGPTI
Sbjct: 1 MAVIVWWLLTVVVVAFHSASAAVVEST----WEVEYKYWWPDCKEGIVMAINGQFPGPTI 60
Query: 72 RANVGDTIIVEMINKLTTESVVIHWHGILQKETPWADGTASISQCATNPGETYLYRFVVD 131
A GDT+I+ ++NKL+TE VVIHWHGI QK TPWADG A ++QC NPGET+ Y+F+VD
Sbjct: 61 DAVAGDTVIIHVVNKLSTEGVVIHWHGIRQKGTPWADGAAGVTQCPINPGETFTYKFIVD 120
Query: 132 KAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFQYDEDINLLLSDWWHKSVYKQEAGL 191
KAGT+FYHGH GMQR++GLYG LIV KE YD + NLLLSDWWH+S++ QE L
Sbjct: 121 KAGTHFYHGHYGMQRSSGLYGMLIV---RSPKERLIYDGEFNLLLSDWWHQSIHAQELAL 180
Query: 192 SSSPMRWIGEPESILINGRGQTNCSIAAKYKKGVEQ--CKLKGSEECAPFILKVKPNKTY 251
SS PMRWIGEP+S+LINGRGQ NCS AA + KG E+ C K +++CAP L+V+PN+ Y
Sbjct: 181 SSRPMRWIGEPQSLLINGRGQFNCSQAAYFNKGGEKDVCTFKENDQCAPQTLRVEPNRVY 240
Query: 252 RIRIASTTSLSALNFAIGNHQLLLVEADGNYVQPFLTSDIDIYSGESYSVLITTDQTPTE 311
R+RIASTT+L++LN A+ HQL++VEADGNYV PF +DID+YSGE+YSVL+ T+ P++
Sbjct: 241 RLRIASTTALASLNLAVQGHQLVVVEADGNYVAPFTVNDIDVYSGETYSVLLKTNALPSK 300
Query: 312 NYWVSIGVRGRKPKTPPALTLLNYLPNSVSKLPISPPPETPQWDDYDHSKNFTYKILATM 371
YW+S+GVRGR+PKTP ALT++NY+ + S+ P PPP TP W+D D SK+F+ KI A
Sbjct: 301 KYWISVGVRGREPKTPQALTVINYVDATESR-PSHPPPVTPIWNDTDRSKSFSKKIFAAK 360
Query: 372 GSPKPPVKSNRRIFLLNTQNNMSGGFTKWAINNVSLALPPTPYLAAIRIGLNDTFD-QNP 431
G PKPP KS+ ++ LLNTQN + +TKW+INNVSL++P TPYL +IR GL +D ++P
Sbjct: 361 GYPKPPEKSHDQLILLNTQN-LYEDYTKWSINNVSLSVPVTPYLGSIRYGLKSAYDLKSP 420
Query: 432 PPETFPEDYDIYTPP-STSTRTGNGIYRFELGEIVDVILQNANTLEKDRSEIHPWHLHGH 491
+ ++YDI PP + +T G+GIY F G +VDVILQNAN L+ SEIHPWH+HGH
Sbjct: 421 AKKLIMDNYDIMKPPPNPNTTKGSGIYNFAFGIVVDVILQNANVLKGVISEIHPWHIHGH 480
Query: 492 DFWVLGYGDGKFSSDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPFHCHIEP 551
DFWVLGYG+GKF +DEK NLKNPPLRNTVV++P+GWTAIRFV DNPGVW FHCHIEP
Sbjct: 481 DFWVLGYGEGKFKPGIDEKTFNLKNPPLRNTVVLYPFGWTAIRFVTDNPGVWFFHCHIEP 540
Query: 552 HLHMGMGVVFAEGVHKVKR--IPTKALACGETAKVLIN 584
HLHMGMGVVF EGV ++ + IP +AL CG T K L+N
Sbjct: 541 HLHMGMGVVFVEGVDRIGKMEIPDEALGCGLTRKWLMN 569
BLAST of Lsi06G004510 vs. TAIR10
Match:
AT4G39830.1 (AT4G39830.1 Cupredoxin superfamily protein)
HSP 1 Score: 563.5 bits (1451), Expect = 1.5e-160
Identity = 271/553 (49.01%), Postives = 361/553 (65.28%), Query Frame = 1
Query: 39 RHYKWEIENMFWSPDCTENLVMGINGQFPGPTIRANVGDTIIVEMINKLTTESVVIHWHG 98
R +KWE++ F SPDC E LV+ ING+FPGPTI+A GDTI+VE+ N TE+V +HWHG
Sbjct: 36 RRFKWEVKYEFKSPDCFEKLVITINGKFPGPTIKAQQGDTIVVELKNSFMTENVAVHWHG 95
Query: 99 ILQKETPWADGTASISQCATNPGETYLYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVDL 158
I Q TPW DG ++QC PGE ++Y+FVVD+ GTY YH H GMQR +GL G + V
Sbjct: 96 IRQIGTPWFDGVEGVTQCPILPGEVFIYQFVVDRPGTYMYHSHYGMQRESGLIGMIQVSP 155
Query: 159 EEGKKEPFQYDEDINLLLSDWWHKSVYKQEAGLSSSPMRWIGEPESILINGRGQTNCS-- 218
+ EPF YD D N LL+DW+HKS+ ++ GL+S P +W+GEP+S++I GRG+ NCS
Sbjct: 156 PATEPEPFTYDYDRNFLLTDWYHKSMSEKATGLASIPFKWVGEPQSLMIQGRGRFNCSNN 215
Query: 219 --IAAKYKKGVEQCKLKGSEECAPFILKVKPNKTYRIRIASTTSLSALNFAIGNHQLLLV 278
GV C + + +C+ FIL V P KTYR+RI S T+LSAL+F I H L +V
Sbjct: 216 LTTPPSLVSGV--CNVSNA-DCSRFILTVIPGKTYRLRIGSLTALSALSFQIEGHNLTVV 275
Query: 279 EADGNYVQPFLTSDIDIYSGESYSVLITTDQTPTENYWVSIGVRGRKPKTPPALTLLNYL 338
EADG+YV+PF ++ +YSGE+YSVL+ DQ P NYW++ + R TPPA +LNY
Sbjct: 276 EADGHYVEPFTVKNLFVYSGETYSVLLKADQNPRRNYWITSSIVSRPATTPPATAVLNYY 335
Query: 339 PNSVSKLPISPPPET----PQWDDYDHSKNFTYKILATMGSPKP-PVKSNRRIFLLNTQN 398
PN + P P E+ P+W+D + I A G P S++ I LLNTQN
Sbjct: 336 PNHPRRRP--PTSESSNIVPEWNDTRSRLAQSLAIKARRGFIHALPENSDKVIVLLNTQN 395
Query: 399 NMSGGFTKWAINNVSLALPPTPYLAAIRIGLNDTFD-QNPPPETF-PEDYDIYTPP-STS 458
++ G+ +W++NNVS P TPYL A++ L + FD + PE + +YDI+ P + +
Sbjct: 396 EVN-GYRRWSVNNVSYHHPKTPYLIALKQNLTNAFDWRFTAPENYDSRNYDIFAKPLNAN 455
Query: 459 TRTGNGIYRFELGEIVDVILQNANTLEKDRSEIHPWHLHGHDFWVLGYGDGKFSSDVDEK 518
T +GIYR VDVILQNANT+ + SE HPWHLHGHDFWVLGYG+GKF+ D K
Sbjct: 456 ATTSDGIYRLRFNSTVDVILQNANTMNANNSETHPWHLHGHDFWVLGYGEGKFNESEDPK 515
Query: 519 KLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPFHCHIEPHLHMGMGVVFAEGVHKVKR 578
+ N +P +NTV + P+GWTA+RF ADNPGVW FHCHIE H MGMG+VF G+ KV
Sbjct: 516 RYNRVDPIKKNTVAVQPFGWTALRFRADNPGVWSFHCHIESHFFMGMGIVFESGIDKVSS 575
Query: 579 IPTKALACGETAK 580
+P+ + CG+T +
Sbjct: 576 LPSSIMGCGQTKR 582
BLAST of Lsi06G004510 vs. TAIR10
Match:
AT5G03260.1 (AT5G03260.1 laccase 11)
HSP 1 Score: 266.2 bits (679), Expect = 4.9e-71
Identity = 177/565 (31.33%), Postives = 281/565 (49.73%), Query Frame = 1
Query: 12 MPFLVIFLLFSIISGFGITLSEAVSQTRHYKWEIENMFWSPDCTENLVMGINGQFPGPTI 71
M FL +F G+ S + + Y+++++ S C ++ +NG FPGPT+
Sbjct: 3 MGFLFLFCYLLAFLGY----SPVDAAVKKYQFDVQVKNISRICNAKPIVTVNGMFPGPTV 62
Query: 72 RANVGDTIIVEMINKLTTESVVIHWHGILQKETPWADGTASISQCATNPGETYLYRF-VV 131
A GD +I+ + N + ++ IHWHG+ Q WADG A I+QC G++YLY F V
Sbjct: 63 YAREGDRVIINVTNHVQY-NMSIHWHGLKQYRNGWADGPAYITQCPIQTGQSYLYDFNVT 122
Query: 132 DKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPF-QYDEDINLLLSDWWHKSVYKQEA 191
+ GT ++H H+ RA +YG++++ GK PF Q ++ N++L +WW+K V E
Sbjct: 123 GQRGTLWWHAHILWLRAT-VYGAIVILPAPGKPYPFPQPYQESNIILGEWWNKDV---ET 182
Query: 192 GLSSSPMRWIGEP--ESILINGRGQTNCSIAAKYKKGVEQCKLKGSEECAPFILKVKPNK 251
++ + P ++ ING+ + K+ F+++ + K
Sbjct: 183 AVNQANQLGAPPPMSDAHTINGKPGPLFPCSEKH----------------TFVIEAEAGK 242
Query: 252 TYRIRIASTTSLSALNFAIGNHQLLLVEADGNYVQPFLTSDIDIYSGESYSVLITTDQTP 311
TY +RI + L F I H + +VE D Y +PF T I + G++ +VL+ TD++P
Sbjct: 243 TYLLRIINAALNDELFFGIAGHNMTVVEIDAVYTKPFTTKAILLGPGQTTNVLVKTDRSP 302
Query: 312 TENYWVSIGVRGRKP-----KTPPALTLLNYLPNSVSKLPISPPPETPQWDDYDHSKNFT 371
Y+++ P KT A+ +PN+V LPI P P + N
Sbjct: 303 -NRYFMAASPFMDAPVSVDNKTVTAILQYKGVPNTV--LPILPKLPLPNDTSFALDYNGK 362
Query: 372 YKILATMGSPK-PPVKSNRRIF------LLNTQNNMSGGFTKWAINNVSLALPPTPYLAA 431
K L T P P+K +RR+F + ++G +INN++ +P T L A
Sbjct: 363 LKSLNTPNFPALVPLKVDRRLFYTIGLGINACPTCVNGTNLAASINNITFIMPKTALLKA 422
Query: 432 IRIGLNDTFDQNPPPETFPEDYDIYTPPSTS---TRTGNGIYRFELGEIVDVILQNANTL 491
++ F + P+ P+ ++ P T+ T TG + R + ++++LQ+ N L
Sbjct: 423 HYSNISGVF-RTDFPDRPPKAFNYTGVPLTANLGTSTGTRLSRVKFNTTIELVLQDTNLL 482
Query: 492 EKDRSEIHPWHLHGHDFWVLGYGDGKFSSDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFV 551
E HP+HLHG++F+V+G G G F D K NL +PP RNTV + GW AIRF
Sbjct: 483 ---TVESHPFHLHGYNFFVVGTGVGNFDPKKDPAKFNLVDPPERNTVGVPTGGWAAIRFR 535
Query: 552 ADNPGVWPFHCHIEPHLHMGMGVVF 558
ADNPGVW HCH+E H G+ + F
Sbjct: 543 ADNPGVWFMHCHLEVHTMWGLKMAF 535
BLAST of Lsi06G004510 vs. TAIR10
Match:
AT5G05390.1 (AT5G05390.1 laccase 12)
HSP 1 Score: 258.8 bits (660), Expect = 7.9e-69
Identity = 172/569 (30.23%), Postives = 274/569 (48.15%), Query Frame = 1
Query: 21 FSIISGFGITLSEA--VSQTRHYKWEIENMFWSPDCTENLVMGINGQFPGPTIRANVGDT 80
FSI+ F S + +++ +H+ + I+ C + +NG FPGPT+ N GDT
Sbjct: 7 FSILLFFCSLFSASLIIAKVQHHDFVIQETPVKRLCKTRNAITVNGMFPGPTLEVNNGDT 66
Query: 81 IIVEMINKLTTESVVIHWHGILQKETPWADGTASISQCATNPGETYLYRFVVD-KAGTYF 140
+ V++ N+ ++ IHWHG+ Q T WADG ++QC PG++Y YRF + + GT +
Sbjct: 67 LEVKVHNR-ARYNITIHWHGVRQIRTGWADGPEFVTQCPIRPGKSYTYRFTIQGQEGTLW 126
Query: 141 YHGHLGMQRAAGLYGSLIVDLEEGKKEPF-QYDEDINLLLSDWWHKS---VYKQEAGLSS 200
+H H RA +YG+LI+ G PF + D L+L +WW+ + V Q +
Sbjct: 127 WHAHSSWLRAT-VYGALIIHPTPGSSFPFPKPDRQTALMLGEWWNANPVDVINQATRTGA 186
Query: 201 SPMRWIGEPESILINGRGQT--NCSIAAKYKKGVEQCKLKGSEECAPFILKVKPNKTYRI 260
+P ++ ING+ NCS K+ V ++ + +T +
Sbjct: 187 AP----NISDAYTINGQPGDLYNCST----KETV--------------VVPINSGETSLL 246
Query: 261 RIASTTSLSALNFAIGNHQLLLVEADGNYVQPFLTSDIDIYSGESYSVLITTDQTPTENY 320
R+ + L F + NH+L +V AD +Y++PF T + + G++ VL+T DQ P + Y
Sbjct: 247 RVINAALNQPLFFTVANHKLTVVGADASYLKPFTTKVLMLGPGQTTDVLLTADQ-PPKRY 306
Query: 321 WVSIGVRGRKPKTP----PALTLLNYLPNSVSKLPISPPPETPQWDDYDHSKNFTYKILA 380
+++ P +L Y + + PI P P ++D + +F+ K +
Sbjct: 307 YIAARAYQSAQNAPFDNTTTTAILQYKKTTTTSKPIM--PVLPAFNDTNTVTSFSRKFKS 366
Query: 381 TMGSPKPPVKSNRRIFLL-------------NTQNNMSGGFTKWAINNVSLALPPT-PYL 440
P + F + + ++G ++NNVS LP L
Sbjct: 367 LRNVVVPKTIDDNLFFTIGLGLDNCPKKFPKSRCQGLNGTRFTASMNNVSFVLPSNFSLL 426
Query: 441 AAIRIGLNDTFDQNPPPETFPEDYDIYTPPSTSTR-----TGNGIYRFELGEIVDVILQN 500
A G+ F + P + P +D YT + S G +Y+ + G V V+LQ+
Sbjct: 427 QAHSNGIPGVFTTDFPSKP-PVKFD-YTGNNISRALFQPVKGTKLYKLKYGSRVQVVLQD 486
Query: 501 ANTLEKDRSEIHPWHLHGHDFWVLGYGDGKFSSDVDEKKLNLKNPPLRNTVVIFPYGWTA 558
N + SE HP HLHG+DF+++G G G F+ D K NL +PPLRNTV + GW
Sbjct: 487 TNIV---TSENHPIHLHGYDFYIVGEGFGNFNPKKDTSKFNLVDPPLRNTVAVPVNGWAV 543
BLAST of Lsi06G004510 vs. NCBI nr
Match:
gi|662033846|ref|NP_001284440.1| (L-ascorbate oxidase-like [Cucumis melo])
HSP 1 Score: 1054.3 bits (2725), Expect = 7.9e-305
Identity = 490/588 (83.33%), Postives = 535/588 (90.99%), Query Frame = 1
Query: 3 KLANQPLFSMPFLVIFLLFSIISGFGITLSEAVSQTRHYKWEIENMFWSPDCTENLVMGI 62
K+ANQPLF MPF IF +FSI GFGITLS+A SQTRH+KWE+E MF SPDC ENLVMGI
Sbjct: 4 KVANQPLFPMPFPAIFFIFSIFFGFGITLSQAASQTRHFKWEVEYMFRSPDCKENLVMGI 63
Query: 63 NGQFPGPTIRANVGDTIIVEMINKLTTESVVIHWHGILQKETPWADGTASISQCATNPGE 122
N QFPGPTIRANVGDT++VE+INKL+TE VVIHWHGILQK TPWADGTASISQCA NPGE
Sbjct: 64 NHQFPGPTIRANVGDTVVVELINKLSTEDVVIHWHGILQKGTPWADGTASISQCAINPGE 123
Query: 123 TYLYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFQYDEDINLLLSDWWHK 182
++ Y+FVVDK GTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPF YDE+INLLLSDWWH+
Sbjct: 124 SFTYQFVVDKPGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEEINLLLSDWWHE 183
Query: 183 SVYKQEAGLSSSPMRWIGEPESILINGRGQTNCSIAAKYKKGVEQCKLKGSEECAPFILK 242
SV KQE GLSSSPMRWIGEP+SILINGRGQ+NCSI AKY K +EQCKL GSEECAPFILK
Sbjct: 184 SVLKQEVGLSSSPMRWIGEPQSILINGRGQSNCSIGAKYMKNMEQCKLSGSEECAPFILK 243
Query: 243 VKPNKTYRIRIASTTSLSALNFAIGNHQLLLVEADGNYVQPFLTSDIDIYSGESYSVLIT 302
VKPNKTYRIRI STTSLSALNFAIGNH+LL+VEADGNYVQPFLTSDIDIYSGESYSVLI
Sbjct: 244 VKPNKTYRIRIISTTSLSALNFAIGNHKLLVVEADGNYVQPFLTSDIDIYSGESYSVLIN 303
Query: 303 TDQTPTENYWVSIGVRGRKPKTPPALTLLNYLPNSVSKLPISPPPETPQWDDYDHSKNFT 362
TDQ P++NYWVS+GVRGR+PKTPP LTLLNYLPNS SKLPISPPPETP+W+DYD SKNFT
Sbjct: 304 TDQNPSQNYWVSLGVRGREPKTPPGLTLLNYLPNSFSKLPISPPPETPKWNDYDRSKNFT 363
Query: 363 YKILATMGSPKPPVKSNRRIFLLNTQNNMSGGFTKWAINNVSLALPPTPYLAAIRIGLND 422
Y+ILA MGSPKPPVKS RRIFLLNTQN M+GG+TKWAINNVSL LP TPYL AI+IG+ND
Sbjct: 364 YRILAAMGSPKPPVKSTRRIFLLNTQNKMNGGYTKWAINNVSLTLPSTPYLGAIKIGIND 423
Query: 423 TFDQNPPPETFPEDYDIY-TPPSTSTRTGNGIYRFELGEIVDVILQNANTLEKDRSEIHP 482
TFDQNPPPET+PE+YDI+ PP+ +T TGNG+YRF+LGEIVDV+LQNANTL SEIHP
Sbjct: 424 TFDQNPPPETYPENYDIHKPPPNGNTITGNGVYRFQLGEIVDVVLQNANTLNDKHSEIHP 483
Query: 483 WHLHGHDFWVLGYGDGKFSSDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPF 542
WHLHGHDFWVLGYG+GKFS+ VDE KLNLK+PPLRNTVVIFP+GWT +RFVADNPGVW F
Sbjct: 484 WHLHGHDFWVLGYGEGKFSAAVDETKLNLKDPPLRNTVVIFPFGWTVLRFVADNPGVWAF 543
Query: 543 HCHIEPHLHMGMGVVFAEGVHKVKRIPTKALACGETAKVLINNPTKKP 590
HCHIEPHLHMGMGVVFAEGV KV RIPT A+ACG TAK LI NPT+KP
Sbjct: 544 HCHIEPHLHMGMGVVFAEGVDKVGRIPTHAMACGGTAKALIQNPTQKP 591
BLAST of Lsi06G004510 vs. NCBI nr
Match:
gi|778663482|ref|XP_011660095.1| (PREDICTED: LOW QUALITY PROTEIN: L-ascorbate oxidase-like [Cucumis sativus])
HSP 1 Score: 1039.6 bits (2687), Expect = 2.0e-300
Identity = 485/590 (82.20%), Postives = 531/590 (90.00%), Query Frame = 1
Query: 3 KLANQPLFSMPFLVIFLLFSIISGFGITLSEAVSQTRHYKWEIENMFWSPDCTENLVMGI 62
K+ANQPLF MPF +F L SII GFGITLS+AVSQTRHY WE+E MFWSPDC ENLVMGI
Sbjct: 4 KVANQPLFPMPFPAMFFLLSIIFGFGITLSDAVSQTRHYTWEVEYMFWSPDCKENLVMGI 63
Query: 63 NGQFPGPTIRANVGDTIIVEMINKLTTESVVIHWHGILQKETPWADGTASISQCATNPGE 122
NGQFPGPTIRANVGDT++VEMINKL+TE VVIHWHGILQK TPWADGTASISQCATNPGE
Sbjct: 64 NGQFPGPTIRANVGDTVVVEMINKLSTEDVVIHWHGILQKGTPWADGTASISQCATNPGE 123
Query: 123 TYLYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFQYDEDINLLLSDWWHK 182
++ Y+FVVDK GTYFYHGHLGMQRAAGLYGSLIVD EEG+KEPF YDE+INLLLSDWWH+
Sbjct: 124 SFTYQFVVDKPGTYFYHGHLGMQRAAGLYGSLIVDPEEGEKEPFHYDEEINLLLSDWWHE 183
Query: 183 SVYKQEAGLSSSPMRWIGEPESILINGRGQTNCSIAAKY--KKGVEQCKLKGSEECAPFI 242
S+ KQE GLSSSPMRWIGEP+SILINGRGQ+NCSIAAKY K +EQCKL GSE+CAPFI
Sbjct: 184 SILKQEIGLSSSPMRWIGEPQSILINGRGQSNCSIAAKYMNMKKMEQCKLSGSEQCAPFI 243
Query: 243 LKVKPNKTYRIRIASTTSLSALNFAIGNHQLLLVEADGNYVQPFLTSDIDIYSGESYSVL 302
L+VKPNK YRIRI STTSLSALNFAIG H+LL+VEADGNYVQPFLTSDIDIYSGESYSVL
Sbjct: 244 LEVKPNKIYRIRITSTTSLSALNFAIGKHKLLVVEADGNYVQPFLTSDIDIYSGESYSVL 303
Query: 303 ITTDQTPTENYWVSIGVRGRKPKTPPALTLLNYLPNSVSKLPISPPPETPQWDDYDHSKN 362
I TDQ P+ENYWVS+G+RGR PKTPP LT+LNYLPN VSKLP SPPPETP+W+DYD SKN
Sbjct: 304 INTDQKPSENYWVSLGIRGRDPKTPPGLTILNYLPNPVSKLPTSPPPETPKWNDYDRSKN 363
Query: 363 FTYKILATMGSPKPPVKSNRRIFLLNTQNNMSGGFTKWAINNVSLALPPTPYLAAIRIGL 422
FT++ILA MGSPKPP+KS RRIFLLNTQN M+GG+TKWAINNVSLALP TPYL AI+IGL
Sbjct: 364 FTFQILAAMGSPKPPLKSTRRIFLLNTQNKMNGGYTKWAINNVSLALPSTPYLGAIKIGL 423
Query: 423 NDTFDQNPPPETFPEDYDI-YTPPSTSTRTGNGIYRFELGEIVDVILQNANTLEKDRSEI 482
N TFDQNPPPET+PEDYDI PP+ +T TGNG+YRF++GEIVDV+LQNANTL SEI
Sbjct: 424 NHTFDQNPPPETYPEDYDINKPPPNRNTITGNGVYRFQIGEIVDVVLQNANTLNDKHSEI 483
Query: 483 HPWHLHGHDFWVLGYGDGKFSSDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVW 542
HPWHL GHDFWVLGYG+GKFSS DE KLNLKNPPLRNTVVIFP+GWT +RFVADNPGV
Sbjct: 484 HPWHLPGHDFWVLGYGEGKFSSAADETKLNLKNPPLRNTVVIFPFGWTVLRFVADNPGVX 543
Query: 543 PFHCHIEPHLHMGMGVVFAEGVHKVKRIPTKALACGETAKVLINNPTKKP 590
FHCHIEPHLHMGMGV+FAEGVHKV RIPT A+ACG TAK LI NPT+KP
Sbjct: 544 AFHCHIEPHLHMGMGVIFAEGVHKVGRIPTNAMACGGTAKALIRNPTQKP 593
BLAST of Lsi06G004510 vs. NCBI nr
Match:
gi|661902949|ref|NP_001284441.1| (L-ascorbate oxidase [Cucumis melo])
HSP 1 Score: 978.0 bits (2527), Expect = 7.2e-282
Identity = 459/591 (77.66%), Postives = 513/591 (86.80%), Query Frame = 1
Query: 1 MAKLANQPLFSMPFLVIFLLFSIISGFGITLSEA-VSQTRHYKWEIENMFWSPDCTENLV 60
MAK+A++P F PFLV F+L SII GFGITLSEA + RHYKWE+E MFWSPDC EN+V
Sbjct: 1 MAKVADKPFFPKPFLVFFVL-SIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIV 60
Query: 61 MGINGQFPGPTIRANVGDTIIVEMINKLTTESVVIHWHGILQKETPWADGTASISQCATN 120
MGINGQFPGPTIRAN GD ++VE+ NKL TE VVIHWHGILQ+ TPWADGTASISQCA N
Sbjct: 61 MGINGQFPGPTIRANAGDMVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAIN 120
Query: 121 PGETYLYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFQYDEDINLLLSDW 180
PGET+ YRFVVDKAGTYFYHGHLGMQR+AGLYGSLIVD +EG EPF YDE+INLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPQEGTSEPFHYDEEINLLLSDW 180
Query: 181 WHKSVYKQEAGLSSSPMRWIGEPESILINGRGQTNCSIAAKYKKGVEQCKLKGSEECAPF 240
WH+SV+KQE GLSS PMRWIGEP+SILINGRGQ +CSIAAKYK G++QC+L G E+CAPF
Sbjct: 181 WHQSVHKQEVGLSSKPMRWIGEPQSILINGRGQFDCSIAAKYKNGLKQCELSGKEQCAPF 240
Query: 241 ILKVKPNKTYRIRIASTTSLSALNFAIGNHQLLLVEADGNYVQPFLTSDIDIYSGESYSV 300
IL V P K YRIRIASTT+L++LNFAIGNH+LL+VEADGNYVQPF TSDIDIYSGESYSV
Sbjct: 241 ILHVHPKKIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFSTSDIDIYSGESYSV 300
Query: 301 LITTDQTPTENYWVSIGVRGRKPKTPPALTLLNYLPNSVSKLPISPPPETPQWDDYDHSK 360
LITTDQ P+ENYWVSIGVR R PKTPP LT+LNYLPNSVSKLPISPPPETP W+D+D SK
Sbjct: 301 LITTDQNPSENYWVSIGVRARLPKTPPGLTVLNYLPNSVSKLPISPPPETPDWEDFDRSK 360
Query: 361 NFTYKILATMGSPKPPVKSNRRIFLLNTQNNMSGGFTKWAINNVSLALPPTPYLAAIRIG 420
NFT++I A MGSPKPPV+ NRR+FLLNTQN ++ GF KWAINNVSLALPPTPYLAA++
Sbjct: 361 NFTFRIFAAMGSPKPPVRYNRRLFLLNTQNRIN-GFMKWAINNVSLALPPTPYLAAMKFR 420
Query: 421 LNDTFDQNPPPETFPEDYDIYTPP-STSTRTGNGIYRFELGEIVDVILQNANTLEKDRSE 480
L+ F+QNPPPETFP DYDI PP + T TGNG+Y+F +GE VDVILQNAN L + SE
Sbjct: 421 LSTAFNQNPPPETFPIDYDINNPPRNPETATGNGVYKFNMGETVDVILQNANMLNANTSE 480
Query: 481 IHPWHLHGHDFWVLGYGDGKFSSDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
IHPWHLHGHDFWVLGYG+GKFS+ D +KLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFSAAEDGRKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
Query: 541 WPFHCHIEPHLHMGMGVVFAEGVHKVKRIPTKALACGETAKVLINNPTKKP 590
W FHCHIEPHLHMGMGV+FAEGVHKV IP A+ACG TA +I N +KP
Sbjct: 541 WAFHCHIEPHLHMGMGVIFAEGVHKVGMIPPNAMACGSTA--MIKNYPRKP 587
BLAST of Lsi06G004510 vs. NCBI nr
Match:
gi|449462573|ref|XP_004149015.1| (PREDICTED: L-ascorbate oxidase [Cucumis sativus])
HSP 1 Score: 973.4 bits (2515), Expect = 1.8e-280
Identity = 456/591 (77.16%), Postives = 513/591 (86.80%), Query Frame = 1
Query: 1 MAKLANQPLFSMPFLVIFLLFSIISGFGITLSEA-VSQTRHYKWEIENMFWSPDCTENLV 60
MAK+A++P F PFL FL+ SII GFGITLSEA + +HYKW++E MFWSPDC EN+V
Sbjct: 1 MAKVADKPFFPKPFLS-FLVLSIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIV 60
Query: 61 MGINGQFPGPTIRANVGDTIIVEMINKLTTESVVIHWHGILQKETPWADGTASISQCATN 120
MGING+FPGPTIRAN GD ++VE+ NKL TE VVIHWHGILQ+ TPWADGTASISQCA N
Sbjct: 61 MGINGEFPGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAIN 120
Query: 121 PGETYLYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFQYDEDINLLLSDW 180
PGET+ YRFVVDKAGTYFYHGHLGMQR+AGLYGSLIVD EG+ EPF YDE+INLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDW 180
Query: 181 WHKSVYKQEAGLSSSPMRWIGEPESILINGRGQTNCSIAAKYKKGVEQCKLKGSEECAPF 240
WH+SV+KQE GLSS PMRWIGEP+SILING+GQ +CSIAAKY +G++QC+L G E+CAPF
Sbjct: 181 WHQSVHKQEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPF 240
Query: 241 ILKVKPNKTYRIRIASTTSLSALNFAIGNHQLLLVEADGNYVQPFLTSDIDIYSGESYSV 300
IL V+P KTYRIRIASTT+L++LNFAIGNH+LL+VEADGNYVQPF+TSDIDIYSGESYSV
Sbjct: 241 ILHVQPKKTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSV 300
Query: 301 LITTDQTPTENYWVSIGVRGRKPKTPPALTLLNYLPNSVSKLPISPPPETPQWDDYDHSK 360
LITTDQ P ENYWVSIGVR R PKTPP LTLLNYLPNS SKLPISPPPETP W+D+D SK
Sbjct: 301 LITTDQNPLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRSK 360
Query: 361 NFTYKILATMGSPKPPVKSNRRIFLLNTQNNMSGGFTKWAINNVSLALPPTPYLAAIRIG 420
NFT++I A MGSPKPPV+ NRR+FLLNTQN ++ GF KWAINNVSLALPPTPYLAA+++
Sbjct: 361 NFTFRIFAAMGSPKPPVRYNRRLFLLNTQNRIN-GFMKWAINNVSLALPPTPYLAAMKMR 420
Query: 421 LNDTFDQNPPPETFPEDYDIYT-PPSTSTRTGNGIYRFELGEIVDVILQNANTLEKDRSE 480
LN F+QNPPPETFP +YDI PP+ T TGNG+Y+F +GE VDVILQNAN L + SE
Sbjct: 421 LNTAFNQNPPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSE 480
Query: 481 IHPWHLHGHDFWVLGYGDGKFSSDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
IHPWHLHGHDFWVLGYG+GKF + DEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
Query: 541 WPFHCHIEPHLHMGMGVVFAEGVHKVKRIPTKALACGETAKVLINNPTKKP 590
W FHCHIEPHLHMGMGVVFAEGVH V IP KALACG TA L+ N + P
Sbjct: 541 WAFHCHIEPHLHMGMGVVFAEGVHMVGMIPPKALACGSTA--LVKNYPRLP 587
BLAST of Lsi06G004510 vs. NCBI nr
Match:
gi|315466374|emb|CBY84386.1| (ascorbate oxidase [Cucumis sativus])
HSP 1 Score: 971.5 bits (2510), Expect = 6.8e-280
Identity = 455/591 (76.99%), Postives = 513/591 (86.80%), Query Frame = 1
Query: 1 MAKLANQPLFSMPFLVIFLLFSIISGFGITLSEA-VSQTRHYKWEIENMFWSPDCTENLV 60
MAK+A++P F PFL FL+ SII GFGITLSEA + +HYKW++E MFWSPDC EN+V
Sbjct: 1 MAKVADKPFFPKPFLS-FLVLSIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIV 60
Query: 61 MGINGQFPGPTIRANVGDTIIVEMINKLTTESVVIHWHGILQKETPWADGTASISQCATN 120
MGING+FPGPTIRAN GD ++VE+ NKL TE VVIHWHGILQ+ TPWADGTASISQCA N
Sbjct: 61 MGINGEFPGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAIN 120
Query: 121 PGETYLYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFQYDEDINLLLSDW 180
PGET+ YRFVVDKAGTYFYHGHLGMQR+AGLYGSLIVD EG+ EPF YDE+INLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDW 180
Query: 181 WHKSVYKQEAGLSSSPMRWIGEPESILINGRGQTNCSIAAKYKKGVEQCKLKGSEECAPF 240
WH+SV+KQE GLSS PMRWIGEP+SILING+GQ +CSIAAKY +G++QC+L G E+CAPF
Sbjct: 181 WHQSVHKQEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPF 240
Query: 241 ILKVKPNKTYRIRIASTTSLSALNFAIGNHQLLLVEADGNYVQPFLTSDIDIYSGESYSV 300
IL V+P KTYRIRIASTT+L++LNFAIGNH+LL+VEADGNYVQPF+TSDIDIYSGESYSV
Sbjct: 241 ILHVQPKKTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSV 300
Query: 301 LITTDQTPTENYWVSIGVRGRKPKTPPALTLLNYLPNSVSKLPISPPPETPQWDDYDHSK 360
LITTDQ P ENYWVSIGVR R PKTPP LTLLNYLPNS SKLPISPPPETP W+D+D SK
Sbjct: 301 LITTDQNPLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRSK 360
Query: 361 NFTYKILATMGSPKPPVKSNRRIFLLNTQNNMSGGFTKWAINNVSLALPPTPYLAAIRIG 420
NFT++I A MGSPKPPV+ NRR+FLLNTQN ++ GF KWAINNVSLALPPTPYLAA+++
Sbjct: 361 NFTFRIFAAMGSPKPPVRYNRRLFLLNTQNRIN-GFMKWAINNVSLALPPTPYLAAMKMR 420
Query: 421 LNDTFDQNPPPETFPEDYDIYT-PPSTSTRTGNGIYRFELGEIVDVILQNANTLEKDRSE 480
LN F+Q+PPPETFP +YDI PP+ T TGNG+Y+F +GE VDVILQNAN L + SE
Sbjct: 421 LNTAFNQSPPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSE 480
Query: 481 IHPWHLHGHDFWVLGYGDGKFSSDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
IHPWHLHGHDFWVLGYG+GKF + DEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
Query: 541 WPFHCHIEPHLHMGMGVVFAEGVHKVKRIPTKALACGETAKVLINNPTKKP 590
W FHCHIEPHLHMGMGVVFAEGVH V IP KALACG TA L+ N + P
Sbjct: 541 WAFHCHIEPHLHMGMGVVFAEGVHMVGMIPPKALACGSTA--LVKNYPRLP 587
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| ASO_CUCSA | 1.1e-282 | 77.16 | L-ascorbate oxidase OS=Cucumis sativus PE=1 SV=1 | [more] |
| ASO_CUCMA | 2.4e-277 | 77.66 | L-ascorbate oxidase OS=Cucurbita maxima GN=AAO PE=1 SV=2 | [more] |
| ASO_CUCPM | 6.1e-273 | 78.22 | L-ascorbate oxidase OS=Cucurbita pepo var. melopepo PE=1 SV=1 | [more] |
| ASO_TOBAC | 1.5e-239 | 66.49 | L-ascorbate oxidase OS=Nicotiana tabacum GN=AAO PE=2 SV=1 | [more] |
| LAC11_ARATH | 8.8e-70 | 31.33 | Laccase-11 OS=Arabidopsis thaliana GN=LAC11 PE=2 SV=1 | [more] |
