Lsi06G003550 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi06G003550
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Pollen Ole e I family allergen
Location	chr06 : 3880292 .. 3881104 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCATAATCTCCACACCTCTCTAATCTAATCTACAAAAGAGCAAAGAAGAAACAAAAAAAAAAAAAAAAGTAAAATCAATGGCGAAATCCACAATCATACTTGTTTCAGCTCTTTGCTTCCTATCGCTCTTTAACTTAGCATCCTGCGATAAGGATCAATTCTTTGTTGAAGGCAAAGTATACTGCGACACATGCCGCGTCCAATTCTTCACAAAAGTCAGTAAATTCCTCGAAGGTAATTAAGCTAACCCTAACCCTAATTCTAGCCCTAAATCGAAATGGAAATTGAAATTGAAATGTGAATTGAAATGTGTTTGTTTATGTAGGTGCGACGGTGAAATTGGAATGCAAAGAAATTGAAGGTGGAAGTGTGACGTTGAGCAAAGAAGCAGTGACTGATAAAACAGGGACTTACAGTATTGAGGCAGATGGAGATCATGAGGAAGAGGTGTGTGAGGTGTCGTTGGTGAAGAGCGAAGATCCTGATTGCAATGAGTTCTCGAAGGAAGGATATGGACATACATCGAGAGTTTCCATCACCAACAACAGTGGAATCACTAATCCTGTTCGTCTTGCCAATCCCCTTGCCTTTTTGAAGAAGGAGAAGCTTACGGAATGCAAAGAGGTTCTTAGAGAGCTCGGATTTGATGAGGAGGGCCTCCCTGTTTAAACTACCCCTCTTTTAATTATTTTTTCTATATTTTATTTCCACCCCTTTTCTTTTTCCTTTCTTATTCACATTAATAATTTTGTCCTCCTCAAATTTTTTATTTCTAATTGAATAATATTTTTTTCTCTTTTATTTATCTTTTAA

mRNA sequence

CCATAATCTCCACACCTCTCTAATCTAATCTACAAAAGAGCAAAGAAGAAACAAAAAAAAAAAAAAAAGTAAAATCAATGGCGAAATCCACAATCATACTTGTTTCAGCTCTTTGCTTCCTATCGCTCTTTAACTTAGCATCCTGCGATAAGGATCAATTCTTTGTTGAAGGCAAAGTATACTGCGACACATGCCGCGTCCAATTCTTCACAAAAGTCAGTAAATTCCTCGAAGGTGCGACGGTGAAATTGGAATGCAAAGAAATTGAAGGTGGAAGTGTGACGTTGAGCAAAGAAGCAGTGACTGATAAAACAGGGACTTACAGTATTGAGGCAGATGGAGATCATGAGGAAGAGGTGTGTGAGGTGTCGTTGGTGAAGAGCGAAGATCCTGATTGCAATGAGTTCTCGAAGGAAGGATATGGACATACATCGAGAGTTTCCATCACCAACAACAGTGGAATCACTAATCCTGTTCGTCTTGCCAATCCCCTTGCCTTTTTGAAGAAGGAGAAGCTTACGGAATGCAAAGAGGTTCTTAGAGAGCTCGGATTTGATGAGGAGGGCCTCCCTGTTTAAACTACCCCTCTTTTAATTATTTTTTCTATATTTTATTTCCACCCCTTTTCTTTTTCCTTTCTTATTCACATTAATAATTTTGTCCTCCTCAAATTTTTTATTTCTAATTGAATAATATTTTTTTCTCTTTTATTTATCTTTTAA

Coding sequence (CDS)

ATGGCGAAATCCACAATCATACTTGTTTCAGCTCTTTGCTTCCTATCGCTCTTTAACTTAGCATCCTGCGATAAGGATCAATTCTTTGTTGAAGGCAAAGTATACTGCGACACATGCCGCGTCCAATTCTTCACAAAAGTCAGTAAATTCCTCGAAGGTGCGACGGTGAAATTGGAATGCAAAGAAATTGAAGGTGGAAGTGTGACGTTGAGCAAAGAAGCAGTGACTGATAAAACAGGGACTTACAGTATTGAGGCAGATGGAGATCATGAGGAAGAGGTGTGTGAGGTGTCGTTGGTGAAGAGCGAAGATCCTGATTGCAATGAGTTCTCGAAGGAAGGATATGGACATACATCGAGAGTTTCCATCACCAACAACAGTGGAATCACTAATCCTGTTCGTCTTGCCAATCCCCTTGCCTTTTTGAAGAAGGAGAAGCTTACGGAATGCAAAGAGGTTCTTAGAGAGCTCGGATTTGATGAGGAGGGCCTCCCTGTTTAA

Protein sequence

MAKSTIILVSALCFLSLFNLASCDKDQFFVEGKVYCDTCRVQFFTKVSKFLEGATVKLECKEIEGGSVTLSKEAVTDKTGTYSIEADGDHEEEVCEVSLVKSEDPDCNEFSKEGYGHTSRVSITNNSGITNPVRLANPLAFLKKEKLTECKEVLRELGFDEEGLPV

Homology

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BLAST of Lsi06G003550 vs. Swiss-Prot
Match: OLEE1_BETPN (Olee1-like protein OS=Betula pendula PE=2 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 2.5e-46
Identity = 92/163 (56.44%), Postives = 121/163 (74.23%), Query Frame = 1


Query: 1   MAKSTIILVSALCFLSLFNLASCDKDQFFVEGKVYCDTCRVQFFTKVSKFLEGATVKLEC 60
           MAKS II   ALCFLSL   A  +  +FFVEGKVYCD CR QF TK+S +++GA V LEC
Sbjct: 1   MAKSIIIQAPALCFLSLLGFAYSES-RFFVEGKVYCDNCRTQFVTKLSTYMKGAKVSLEC 60

Query: 61  KEIEGGSVTLSKEAVTDKTGTYSIEADGDHEEEVCEVSLVKSEDPDCNEFSKEGY-GHTS 120
           +  EGG++  S ++ TDK+GTY I  DGDHEEE+CE++L KS DPDC+E SK+ +   ++
Sbjct: 61  RNREGGTLIYSSDSETDKSGTYRIPVDGDHEEEICEIALKKSSDPDCSEVSKDPFLKKSA 120

Query: 121 RVSITNNSGITNPVRLANPLAFLKKEKLTECKEVLRELGFDEE 163
           R+S+T N+GI+ PVRLANPL F+KK+ L EC + LRELG + +
Sbjct: 121 RISLTKNNGISTPVRLANPLGFMKKKPLPECAKALRELGMNPD 162

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BLAST of Lsi06G003550 vs. Swiss-Prot
Match: CHE1_CHEAL (Pollen allergen Che a 1 OS=Chenopodium album PE=1 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 5.0e-39
Identity = 78/156 (50.00%), Postives = 112/156 (71.79%), Query Frame = 1


Query: 3   KSTIILVSALCFLSLFNLASCDKDQFFVEGKVYCDTCRVQFFTKVSKFLEGATVKLECKE 62
           ++  +LV ALC LSL  +A+  ++ F V+G VYCDTCR+QF T++S  +EGATVKLEC+ 
Sbjct: 5   QAVFLLVGALCVLSLAGVANAAENHFKVQGMVYCDTCRIQFMTRISTIMEGATVKLECRN 64

Query: 63  IEGGSVTLSKEAVTDKTGTYSIEADGDHEEEVCEVSLVKSEDPDCNEFSKEGYG-HTSRV 122
           I  G+ T   EAVTDK G YSI  +GD E+++CE+ LVKS + +C+E S + Y   +++V
Sbjct: 65  ITAGTQTFKAEAVTDKVGQYSIPVNGDFEDDICEIELVKSPNSECSEVSHDVYAKQSAKV 124

Query: 123 SITNNSGITNPVRLANPLAFLKKEKLTECKEVLREL 158
           S+T+N+G  + +R AN L F++KE L EC EVL+EL
Sbjct: 125 SLTSNNGEASDIRSANALGFMRKEPLKECPEVLKEL 160

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BLAST of Lsi06G003550 vs. Swiss-Prot
Match: LAT52_SOLLC (Anther-specific protein LAT52 OS=Solanum lycopersicum GN=LAT52 PE=2 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 5.0e-39
Identity = 88/158 (55.70%), Postives = 110/158 (69.62%), Query Frame = 1


Query: 1   MAKSTIILVSALCFLSLFNLASCDKDQFFVEGKVYCDTCRVQFFTKVSKFLEGATVKLEC 60
           MAK+ I+L+SALC L+L N A C  + F VEGKVYCDTCRVQF TK+S+ LEGATVKL+C
Sbjct: 1   MAKA-IVLLSALCILALANFAHCRPEVFDVEGKVYCDTCRVQFETKLSENLEGATVKLQC 60

Query: 61  KEIEGGSVTLSKEAVTDKTGTYSIEADGDHEEEVCEVSLVKSEDPDCNEFSKEGYGHTSR 120
           + I   + T S E VTDK G Y +  +GDHE ++CEV++VKS   DC E S  GY   +R
Sbjct: 61  RNISTEAETFSVEGVTDKDGKYKLTVNGDHENDICEVTVVKSPREDCKE-SVSGY-EKAR 120

Query: 121 VSITNNSGITNPVRLANPLAFLKKEKLTECKEVLRELG 159
           +  ++N GI N VR ANPL F+K E +  CKE L ELG
Sbjct: 121 IECSDNVGIHNAVRFANPLFFMKAESVQGCKEALDELG 155

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BLAST of Lsi06G003550 vs. Swiss-Prot
Match: OLE96_ARATH (Pollen-specific protein-like At4g18596 OS=Arabidopsis thaliana GN=At4g18596 PE=2 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 7.3e-38
Identity = 82/155 (52.90%), Postives = 109/155 (70.32%), Query Frame = 1


Query: 9   VSALCFLSLFNLASCDKD---QFFVEGKVYCDTCRVQFFTKVSKFLEGATVKLECKEIEG 68
           VSA+C  SL  +A  D D   +F ++G VYCDTCRVQF T++SKFLEGA VKLEC+    
Sbjct: 11  VSAVCLSSLAGVAIADADDFDRFQIQGSVYCDTCRVQFVTRLSKFLEGAKVKLECRSRTN 70

Query: 69  GSVTLSKEAVTDKTGTYSIEADGDHEEEVCEVSLVKSEDPDCNEFSKEGY-GHTSRVSIT 128
           G+VTL+KEAVTDKTG+Y +E  GDHEEEVCE+ LV+S D  C++ SKE Y  + +++S+T
Sbjct: 71  GTVTLTKEAVTDKTGSYRMEVTGDHEEEVCELVLVESPDSGCSDVSKEAYLRNAAKISLT 130

Query: 129 NNSGI-TNPVRLANPLAFLKKEKLTECKEVLRELG 159
            N GI ++  R+ NPL F+ +    EC    +ELG
Sbjct: 131 ANDGIVSHETRIVNPLGFMVQTPSAECPAAFKELG 165

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BLAST of Lsi06G003550 vs. Swiss-Prot
Match: LOLB_LOLPR (Major pollen allergen Lol p 11 OS=Lolium perenne PE=1 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 2.6e-27
Identity = 64/129 (49.61%), Postives = 82/129 (63.57%), Query Frame = 1


Query: 28  FFVEGKVYCDTCRVQFFTKVSKFLEGATVKLECKEIEGGSVTLSKEAVTDKTGTYSIEAD 87
           F V G+VYCD CR  F T VS  +EGATV ++C+  +GG   L  EA TDK G Y IE D
Sbjct: 6   FVVTGRVYCDPCRAGFETNVSHNVEGATVAVDCRPFDGGESKLKAEATTDKDGWYKIEID 65

Query: 88  GDHEEEVCEVSLVKSEDPDCNEFSKEGYGHTSRVSITNNSGITNP-VRLANPLAFLKKEK 147
            DH+EE+CEV L KS D  C+E   E +   +RV +T+N GI    +R ANP+AF +KE 
Sbjct: 66  QDHQEEICEVVLAKSPDKSCSEI--EEFRDRARVPLTSNXGIKQQGIRYANPIAFFRKEP 125

Query: 148 LTECKEVLR 156
           L EC  +L+
Sbjct: 126 LKECGGILQ 132

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BLAST of Lsi06G003550 vs. TrEMBL
Match: A0A0A0LX09_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G597700 PE=4 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 3.1e-80
Identity = 154/166 (92.77%), Postives = 161/166 (96.99%), Query Frame = 1


Query: 1   MAKSTIILVSALCFLSLFNLASCDKDQFFVEGKVYCDTCRVQFFTKVSKFLEGATVKLEC 60
           MAKSTIILVSALCF+SLF+LASC KDQFFVEGKVYCDTCRVQFFTKVSKFLEGATVKLEC
Sbjct: 1   MAKSTIILVSALCFISLFHLASC-KDQFFVEGKVYCDTCRVQFFTKVSKFLEGATVKLEC 60

Query: 61  KEIEGGSVTLSKEAVTDKTGTYSIEADGDHEEEVCEVSLVKSEDPDCNEFSKEGYGHTSR 120
           KEIEGGSVTL KEAVTDK+G YSIEADGDHEEEVCEVSL+KS DPDC+E SKEGYGHTSR
Sbjct: 61  KEIEGGSVTLDKEAVTDKSGKYSIEADGDHEEEVCEVSLMKSVDPDCDEISKEGYGHTSR 120

Query: 121 VSITNNSGITNPVRLANPLAFLKKEKLTECKEVLRELGFDEEGLPV 167
           V+ITNNSGITNPVRLANPLAFLKKEKL+ECKEVLRELGFDEEGLPV
Sbjct: 121 VTITNNSGITNPVRLANPLAFLKKEKLSECKEVLRELGFDEEGLPV 165

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BLAST of Lsi06G003550 vs. TrEMBL
Match: A0A0A0KLB5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G172880 PE=4 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 1.8e-67
Identity = 133/166 (80.12%), Postives = 144/166 (86.75%), Query Frame = 1


Query: 1   MAKSTIILVSALCFLSLFNLASCDKDQFFVEGKVYCDTCRVQFFTKVSKFLEGATVKLEC 60
           MAKS I+ VSAL   SL N+A   KD+F VEGKVYCD CRVQFFTK SKFLEGATVKL C
Sbjct: 1   MAKSIILFVSALSLFSLLNVAYSQKDRFLVEGKVYCDACRVQFFTKASKFLEGATVKLVC 60

Query: 61  KEIEGGSVTLSKEAVTDKTGTYSIEADGDHEEEVCEVSLVKSEDPDCNEFSKEGYGHTSR 120
           KEIEGGSVTLSKEAVTDKTG+YSIEADGDHEE++CEV+LVKS DPDC+E S E Y H +R
Sbjct: 61  KEIEGGSVTLSKEAVTDKTGSYSIEADGDHEEDLCEVTLVKSNDPDCSEISLEKYEHMAR 120

Query: 121 VSITNNSGITNPVRLANPLAFLKKEKLTECKEVLRELGFDEEGLPV 167
           VSITNNSGITNPVR ANPLAF+KKEKL ECKEVLRELGFDEEG+ V
Sbjct: 121 VSITNNSGITNPVRHANPLAFMKKEKLPECKEVLRELGFDEEGILV 166

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BLAST of Lsi06G003550 vs. TrEMBL
Match: A0A0A0KLN9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G519550 PE=4 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 6.6e-54
Identity = 109/168 (64.88%), Postives = 130/168 (77.38%), Query Frame = 1


Query: 1   MAKS-TIILVSALCFLSLFNLASCDKDQFFVEGKVYCDTCRVQFFTKVSKFLEGATVKLE 60
           MAKS  IILVSALCFLS  ++A   KD+FF+EGKVYCDTCR+QFFT+VSK+L GA VKL 
Sbjct: 1   MAKSCAIILVSALCFLSFLDIALSSKDRFFIEGKVYCDTCRIQFFTRVSKYLPGAKVKLV 60

Query: 61  CKE-IEGGSVTLSKEAVTDKTGTYSIEADGDHEEEVCEVSLVKSEDPDCNEFSKEGYGHT 120
           C+E    G+ T + E VTDK G Y IE DGDHEEE+CEVSL++S D  C E   +GYGH 
Sbjct: 61  CREEANAGNETFTGEGVTDKNGVYKIEVDGDHEEEICEVSLLESADTGCGEIPTDGYGHF 120

Query: 121 SRVSITNNSGITNPVRLANPLAFLKKEKLTECKEVLRELGFDEEGLPV 167
           +RVSIT N+GI NPVR ANPL F+KK+ L +CKEVLRELGFD+ G+ V
Sbjct: 121 ARVSITGNNGIINPVRQANPLGFMKKDALPQCKEVLRELGFDDAGILV 168

[Close]

BLAST of Lsi06G003550 vs. TrEMBL
Match: A0A0A0KI96_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G519560 PE=4 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 8.6e-54
Identity = 108/168 (64.29%), Postives = 132/168 (78.57%), Query Frame = 1


Query: 1   MAKS-TIILVSALCFLSLFNLASCDKDQFFVEGKVYCDTCRVQFFTKVSKFLEGATVKLE 60
           MAKS  IILVSALCFLS  ++A   KD+FF+EGKVYCDTCR+QFFT+VSK+L GA VKL 
Sbjct: 1   MAKSCAIILVSALCFLSFLDIALSSKDRFFIEGKVYCDTCRIQFFTRVSKYLPGAKVKLV 60

Query: 61  CKE-IEGGSVTLSKEAVTDKTGTYSIEADGDHEEEVCEVSLVKSEDPDCNEFSKEGYGHT 120
           C+E    G+ T + E VTDK G Y IE DGDHEEE+CEVS+++S D +C++   +GYGH 
Sbjct: 61  CREEANAGNETFTGEGVTDKNGVYKIEVDGDHEEEICEVSVLESADEECSDIPTDGYGHF 120

Query: 121 SRVSITNNSGITNPVRLANPLAFLKKEKLTECKEVLRELGFDEEGLPV 167
           +RVSIT N+GI NPVR ANPL FLKK+ L +CKEVLRELGFD+ G+ V
Sbjct: 121 ARVSITGNNGIINPVRQANPLGFLKKDALPQCKEVLRELGFDDVGILV 168

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BLAST of Lsi06G003550 vs. TrEMBL
Match: W9RK59_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_005787 PE=4 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 8.0e-52
Identity = 105/165 (63.64%), Postives = 123/165 (74.55%), Query Frame = 1


Query: 1   MAKSTIILVSALCFLSLFNLASCDKDQFFVEGKVYCDTCRVQFFTKVSKFLEGATVKLEC 60
           M KS II+ SALCF+SL   A CD     VEG+VYCDTCR QF TKVS +++GA V+LEC
Sbjct: 1   MTKSVIIIASALCFMSLLGFAYCDTPHLIVEGRVYCDTCRAQFVTKVSNYIKGAKVRLEC 60

Query: 61  KEIEGGSVTLSKEAVTDKTGTYSIEADGDHEEEVCEVSLVKSEDPDCNEFSKEGYGH-TS 120
           +E EGGSVT S + VTD++GTY I  DGDHEEEVCEV LVKS  PDC+E S + Y    +
Sbjct: 61  RESEGGSVTYSNDGVTDESGTYRIAVDGDHEEEVCEVKLVKSSVPDCSEISNDPYNKLNA 120

Query: 121 RVSITNNSGITNPVRLANPLAFLKKEKLTECKEVLRELGFDEEGL 165
           RVS+T+N+GIT+PVR ANPL FLKK  L EC EVLRELG   EGL
Sbjct: 121 RVSLTSNNGITSPVRQANPLGFLKKNSLPECPEVLRELGITPEGL 165

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BLAST of Lsi06G003550 vs. TAIR10
Match: AT1G29140.1 (AT1G29140.1 Pollen Ole e 1 allergen and extensin family protein)

HSP 1 Score: 165.2 bits (417), Expect = 3.3e-41
Identity = 86/164 (52.44%), Postives = 115/164 (70.12%), Query Frame = 1


Query: 1   MAKSTII-LVSALCFLSLFNLASCDKD---QFFVEGKVYCDTCRVQFFTKVSKFLEGATV 60
           MAK  I+ L SALCF +L +  + D D   +F ++G VYCDTCRVQF T++SKFLEGA V
Sbjct: 1   MAKFFIVFLASALCFTTLVHFTAADADDFDKFHIKGSVYCDTCRVQFITRISKFLEGAKV 60

Query: 61  KLECKEIEGGSVTLSKEAVTDKTGTYSIEADGDHEEEVCEVSLVKSEDPDCNEFSKEGY- 120
           KLECK  E  +VTL+KEAVTD  G Y +E  GDHEEEVCE+ L++S DP+C + + + + 
Sbjct: 61  KLECKGRENQTVTLTKEAVTDNAGNYQMEVMGDHEEEVCEIVLLQSPDPECGDVNNQEFL 120

Query: 121 GHTSRVSITNNSGI-TNPVRLANPLAFLKKEKLTECKEVLRELG 159
            + +R+S+T N GI +N  R  NPL F++K  L EC +V +ELG
Sbjct: 121 RNAARISLTANDGIVSNETRTINPLGFMRKTPLAECPQVFKELG 164

[Close]

BLAST of Lsi06G003550 vs. TAIR10
Match: AT4G18596.1 (AT4G18596.1 Pollen Ole e 1 allergen and extensin family protein)

HSP 1 Score: 158.3 bits (399), Expect = 4.1e-39
Identity = 82/155 (52.90%), Postives = 109/155 (70.32%), Query Frame = 1


Query: 9   VSALCFLSLFNLASCDKD---QFFVEGKVYCDTCRVQFFTKVSKFLEGATVKLECKEIEG 68
           VSA+C  SL  +A  D D   +F ++G VYCDTCRVQF T++SKFLEGA VKLEC+    
Sbjct: 11  VSAVCLSSLAGVAIADADDFDRFQIQGSVYCDTCRVQFVTRLSKFLEGAKVKLECRSRTN 70

Query: 69  GSVTLSKEAVTDKTGTYSIEADGDHEEEVCEVSLVKSEDPDCNEFSKEGY-GHTSRVSIT 128
           G+VTL+KEAVTDKTG+Y +E  GDHEEEVCE+ LV+S D  C++ SKE Y  + +++S+T
Sbjct: 71  GTVTLTKEAVTDKTGSYRMEVTGDHEEEVCELVLVESPDSGCSDVSKEAYLRNAAKISLT 130

Query: 129 NNSGI-TNPVRLANPLAFLKKEKLTECKEVLRELG 159
            N GI ++  R+ NPL F+ +    EC    +ELG
Sbjct: 131 ANDGIVSHETRIVNPLGFMVQTPSAECPAAFKELG 165

[Close]

BLAST of Lsi06G003550 vs. TAIR10
Match: AT5G45880.1 (AT5G45880.1 Pollen Ole e 1 allergen and extensin family protein)

HSP 1 Score: 157.9 bits (398), Expect = 5.3e-39
Identity = 80/159 (50.31%), Postives = 111/159 (69.81%), Query Frame = 1


Query: 5   TIILVSALCFLSLFNLASCDKD---QFFVEGKVYCDTCRVQFFTKVSKFLEGATVKLECK 64
           +  +VSA+C  SL   A+ D D   +F ++G VYCDTCRVQF T++SKFLEGA VKLEC+
Sbjct: 9   SFFVVSAVCLSSLAGFAAADADDFDRFQIQGSVYCDTCRVQFVTRLSKFLEGAKVKLECR 68

Query: 65  EIEGGSVTLSKEAVTDKTGTYSIEADGDHEEEVCEVSLVKSEDPDCNEFSKEGY-GHTSR 124
               G++TL+KEAVTDKTG+Y +E  GDHEEEVCE+ LV+S D  C++ S E Y  + ++
Sbjct: 69  SRTNGTITLTKEAVTDKTGSYKMEVTGDHEEEVCELVLVQSPDSGCSDVSTEAYLRNAAK 128

Query: 125 VSITNNSGI-TNPVRLANPLAFLKKEKLTECKEVLRELG 159
           +S+T N GI ++  R+ NPL F+ +  L +C    +ELG
Sbjct: 129 ISLTANDGIVSHETRIVNPLGFMVQTPLADCPAAFKELG 167

[Close]

BLAST of Lsi06G003550 vs. TAIR10
Match: AT4G08685.1 (AT4G08685.1 Pollen Ole e 1 allergen and extensin family protein)

HSP 1 Score: 107.1 bits (266), Expect = 1.1e-23
Identity = 60/154 (38.96%), Postives = 91/154 (59.09%), Query Frame = 1


Query: 4   STIILVSALCFLSLFNLASC-DKDQFFVEGKVYCDTCRVQFFTKVSKFLEGATVKLECKE 63
           S  +L+ ALCFL    +A+  +K+ F V G+VYCDTC   F T  S ++ GA V+LECK+
Sbjct: 2   SKAVLLVALCFLPALAIAARPNKNPFVVRGRVYCDTCLAGFETPASTYISGAVVRLECKD 61

Query: 64  IEGGSVTLSKEAVTDKTGTYSIEADGDHEEEVCEVSLVKSEDPDCNEFSKEGYGH-TSRV 123
                +T S EA TD TG+Y I  + DH+E+ C+  LV+S    C+  S    GH  +RV
Sbjct: 62  RRTMELTYSHEARTDSTGSYKILVNEDHDEQFCDAMLVRSSQLRCSNVSP---GHDRARV 121

Query: 124 SITNNSGITNPVRLANPLAFLKKEKLTECKEVLR 156
           ++T  +GI +  R AN + FL+   +  C ++++
Sbjct: 122 TLTRFNGIASDDRFANNMGFLRDAAMPGCADIMK 152

[Close]

BLAST of Lsi06G003550 vs. TAIR10
Match: AT1G78040.1 (AT1G78040.1 Pollen Ole e 1 allergen and extensin family protein)

HSP 1 Score: 89.0 bits (219), Expect = 3.0e-18
Identity = 59/167 (35.33%), Postives = 88/167 (52.69%), Query Frame = 1


Query: 1   MAKSTIILVSALCFLSLFNLAS----CDKDQFFVEGKVYCDTCRVQFFTKVSK-FLEGAT 60
           MAK  ++LV  LC L    +A+      K+   V+G  YCD C+  F T  S  F+ GAT
Sbjct: 1   MAKLVMLLV--LCILPAIAMAARRGNIGKNTMVVQGSTYCDICKFGFETPESSYFIPGAT 60

Query: 61  VKLECKEIEGGSVTLSKEAVTDKTGTYSIEADGDHEEEVCEVSLVKSEDPDCNEFSKEGY 120
           VKL CK+ +      + +AV+DK G Y      DH +++C+V LVKS D  C++ S    
Sbjct: 61  VKLSCKDRKTMEEVYTDKAVSDKEGKYKFIVHDDHRDQMCDVLLVKSSDKTCSKISVG-- 120

Query: 121 GHTSRVSITNNSGITNPVRLANPLAFLKKEKLTECKEVLRELGFDEE 163
              SRV + + SGI + +R AN + F K+     C  + ++   DE+
Sbjct: 121 REKSRVILNHYSGIASQIRHANNMGFEKEVSDVFCSALFQKYMVDED 163

[Close]

BLAST of Lsi06G003550 vs. NCBI nr
Match: gi|449452765|ref|XP_004144129.1| (PREDICTED: olee1-like protein [Cucumis sativus])

HSP 1 Score: 305.8 bits (782), Expect = 4.5e-80
Identity = 154/166 (92.77%), Postives = 161/166 (96.99%), Query Frame = 1


Query: 1   MAKSTIILVSALCFLSLFNLASCDKDQFFVEGKVYCDTCRVQFFTKVSKFLEGATVKLEC 60
           MAKSTIILVSALCF+SLF+LASC KDQFFVEGKVYCDTCRVQFFTKVSKFLEGATVKLEC
Sbjct: 1   MAKSTIILVSALCFISLFHLASC-KDQFFVEGKVYCDTCRVQFFTKVSKFLEGATVKLEC 60

Query: 61  KEIEGGSVTLSKEAVTDKTGTYSIEADGDHEEEVCEVSLVKSEDPDCNEFSKEGYGHTSR 120
           KEIEGGSVTL KEAVTDK+G YSIEADGDHEEEVCEVSL+KS DPDC+E SKEGYGHTSR
Sbjct: 61  KEIEGGSVTLDKEAVTDKSGKYSIEADGDHEEEVCEVSLMKSVDPDCDEISKEGYGHTSR 120

Query: 121 VSITNNSGITNPVRLANPLAFLKKEKLTECKEVLRELGFDEEGLPV 167
           V+ITNNSGITNPVRLANPLAFLKKEKL+ECKEVLRELGFDEEGLPV
Sbjct: 121 VTITNNSGITNPVRLANPLAFLKKEKLSECKEVLRELGFDEEGLPV 165

[Close]

BLAST of Lsi06G003550 vs. NCBI nr
Match: gi|659100147|ref|XP_008450951.1| (PREDICTED: olee1-like protein [Cucumis melo])

HSP 1 Score: 298.5 bits (763), Expect = 7.2e-78
Identity = 151/166 (90.96%), Postives = 158/166 (95.18%), Query Frame = 1


Query: 1   MAKSTIILVSALCFLSLFNLASCDKDQFFVEGKVYCDTCRVQFFTKVSKFLEGATVKLEC 60
           M KSTIIL+SALCF+SLF LAS  KDQFFVEGKVYCDTCRVQFFTKVSKFL+GATVKLEC
Sbjct: 1   MTKSTIILISALCFVSLFYLAS-SKDQFFVEGKVYCDTCRVQFFTKVSKFLKGATVKLEC 60

Query: 61  KEIEGGSVTLSKEAVTDKTGTYSIEADGDHEEEVCEVSLVKSEDPDCNEFSKEGYGHTSR 120
           KEIEGGSVTL+KEAVTDK+G YSIEADGDHEEEVCEVSLVKS DPDC+E SKEGYGHTSR
Sbjct: 61  KEIEGGSVTLNKEAVTDKSGKYSIEADGDHEEEVCEVSLVKSVDPDCDEISKEGYGHTSR 120

Query: 121 VSITNNSGITNPVRLANPLAFLKKEKLTECKEVLRELGFDEEGLPV 167
           V+ITNNSGITNPVRLANPLAFLKKEKL ECKEVLRELGFDEEGLPV
Sbjct: 121 VTITNNSGITNPVRLANPLAFLKKEKLPECKEVLRELGFDEEGLPV 165

[Close]

BLAST of Lsi06G003550 vs. NCBI nr
Match: gi|659073068|ref|XP_008467237.1| (PREDICTED: olee1-like protein [Cucumis melo])

HSP 1 Score: 266.5 bits (680), Expect = 3.0e-68
Identity = 134/166 (80.72%), Postives = 146/166 (87.95%), Query Frame = 1


Query: 1   MAKSTIILVSALCFLSLFNLASCDKDQFFVEGKVYCDTCRVQFFTKVSKFLEGATVKLEC 60
           MAKS I+LVSAL  +SL N+A   KD+F VEGKVYCD CRVQFFTK SKFLEGATVKL C
Sbjct: 1   MAKSMILLVSALSLMSLLNVAFSQKDRFLVEGKVYCDACRVQFFTKASKFLEGATVKLVC 60

Query: 61  KEIEGGSVTLSKEAVTDKTGTYSIEADGDHEEEVCEVSLVKSEDPDCNEFSKEGYGHTSR 120
           KEIEGGSVTLSKEAVTDKTG+YSIEADGDHEEE+CEV+LVKS DPDC+E S E Y H +R
Sbjct: 61  KEIEGGSVTLSKEAVTDKTGSYSIEADGDHEEELCEVTLVKSNDPDCSEISLEKYEHMAR 120

Query: 121 VSITNNSGITNPVRLANPLAFLKKEKLTECKEVLRELGFDEEGLPV 167
           VSITNNSGITNPVR ANPLAF+KKEKL ECKE+LRELGFDEEG+ V
Sbjct: 121 VSITNNSGITNPVRHANPLAFMKKEKLPECKEILRELGFDEEGILV 166

[Close]

BLAST of Lsi06G003550 vs. NCBI nr
Match: gi|449451773|ref|XP_004143635.1| (PREDICTED: olee1-like protein [Cucumis sativus])

HSP 1 Score: 263.5 bits (672), Expect = 2.6e-67
Identity = 133/166 (80.12%), Postives = 144/166 (86.75%), Query Frame = 1


Query: 1   MAKSTIILVSALCFLSLFNLASCDKDQFFVEGKVYCDTCRVQFFTKVSKFLEGATVKLEC 60
           MAKS I+ VSAL   SL N+A   KD+F VEGKVYCD CRVQFFTK SKFLEGATVKL C
Sbjct: 1   MAKSIILFVSALSLFSLLNVAYSQKDRFLVEGKVYCDACRVQFFTKASKFLEGATVKLVC 60

Query: 61  KEIEGGSVTLSKEAVTDKTGTYSIEADGDHEEEVCEVSLVKSEDPDCNEFSKEGYGHTSR 120
           KEIEGGSVTLSKEAVTDKTG+YSIEADGDHEE++CEV+LVKS DPDC+E S E Y H +R
Sbjct: 61  KEIEGGSVTLSKEAVTDKTGSYSIEADGDHEEDLCEVTLVKSNDPDCSEISLEKYEHMAR 120

Query: 121 VSITNNSGITNPVRLANPLAFLKKEKLTECKEVLRELGFDEEGLPV 167
           VSITNNSGITNPVR ANPLAF+KKEKL ECKEVLRELGFDEEG+ V
Sbjct: 121 VSITNNSGITNPVRHANPLAFMKKEKLPECKEVLRELGFDEEGILV 166

[Close]

BLAST of Lsi06G003550 vs. NCBI nr
Match: gi|659078469|ref|XP_008439742.1| (PREDICTED: olee1-like protein isoform X1 [Cucumis melo])

HSP 1 Score: 224.2 bits (570), Expect = 1.7e-55
Identity = 112/168 (66.67%), Postives = 134/168 (79.76%), Query Frame = 1


Query: 1   MAKS-TIILVSALCFLSLFNLASCDKDQFFVEGKVYCDTCRVQFFTKVSKFLEGATVKLE 60
           MAKS  IILVSALCFLS  ++A   KD+FF+EGKVYCDTCR+QFFT+VSK+L GA VKL 
Sbjct: 1   MAKSCAIILVSALCFLSFLDIALSSKDRFFIEGKVYCDTCRIQFFTRVSKYLPGAKVKLV 60

Query: 61  CKE-IEGGSVTLSKEAVTDKTGTYSIEADGDHEEEVCEVSLVKSEDPDCNEFSKEGYGHT 120
           C+E    G+ T + EAVTDK G Y IE DGDHEEE+CEVSL++S D +C+    +GYGH 
Sbjct: 61  CREEANAGNETFTGEAVTDKNGVYKIEVDGDHEEEICEVSLLESADKECSGIPTDGYGHF 120

Query: 121 SRVSITNNSGITNPVRLANPLAFLKKEKLTECKEVLRELGFDEEGLPV 167
           +RVSIT N+GITNPVR ANPL FL+K+ L +CKEVLRELGFDEEG+ V
Sbjct: 121 ARVSITGNNGITNPVRQANPLGFLRKDALPQCKEVLRELGFDEEGILV 168

The following BLAST results are available for this feature:

BLAST of Lsi06G003550 vs. Swiss-Prot


Match Name	E-value	Identity	Description
OLEE1_BETPN	2.5e-46	56.44	Olee1-like protein OS=Betula pendula PE=2 SV=1	[more]
CHE1_CHEAL	5.0e-39	50.00	Pollen allergen Che a 1 OS=Chenopodium album PE=1 SV=1	[more]
LAT52_SOLLC	5.0e-39	55.70	Anther-specific protein LAT52 OS=Solanum lycopersicum GN=LAT52 PE=2 SV=1	[more]
OLE96_ARATH	7.3e-38	52.90	Pollen-specific protein-like At4g18596 OS=Arabidopsis thaliana GN=At4g18596 PE=2...	[more]
LOLB_LOLPR	2.6e-27	49.61	Major pollen allergen Lol p 11 OS=Lolium perenne PE=1 SV=1	[more]

BLAST of Lsi06G003550 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LX09_CUCSA	3.1e-80	92.77	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G597700 PE=4 SV=1	[more]
A0A0A0KLB5_CUCSA	1.8e-67	80.12	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G172880 PE=4 SV=1	[more]
A0A0A0KLN9_CUCSA	6.6e-54	64.88	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G519550 PE=4 SV=1	[more]
A0A0A0KI96_CUCSA	8.6e-54	64.29	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G519560 PE=4 SV=1	[more]
W9RK59_9ROSA	8.0e-52	63.64	Uncharacterized protein OS=Morus notabilis GN=L484_005787 PE=4 SV=1	[more]

BLAST of Lsi06G003550 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G29140.1	3.3e-41	52.44	Pollen Ole e 1 allergen and extensin family protein	[more]
AT4G18596.1	4.1e-39	52.90	Pollen Ole e 1 allergen and extensin family protein	[more]
AT5G45880.1	5.3e-39	50.31	Pollen Ole e 1 allergen and extensin family protein	[more]
AT4G08685.1	1.1e-23	38.96	Pollen Ole e 1 allergen and extensin family protein	[more]
AT1G78040.1	3.0e-18	35.33	Pollen Ole e 1 allergen and extensin family protein	[more]

BLAST of Lsi06G003550 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449452765\|ref\|XP_004144129.1\|	4.5e-80	92.77	PREDICTED: olee1-like protein [Cucumis sativus]	[more]
gi\|659100147\|ref\|XP_008450951.1\|	7.2e-78	90.96	PREDICTED: olee1-like protein [Cucumis melo]	[more]
gi\|659073068\|ref\|XP_008467237.1\|	3.0e-68	80.72	PREDICTED: olee1-like protein [Cucumis melo]	[more]
gi\|449451773\|ref\|XP_004143635.1\|	2.6e-67	80.12	PREDICTED: olee1-like protein [Cucumis sativus]	[more]
gi\|659078469\|ref\|XP_008439742.1\|	1.7e-55	66.67	PREDICTED: olee1-like protein isoform X1 [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Cellular Component**
Term	Definition
GO:0005615	extracellular space

Vocabulary: **INTERPRO**
Term	Definition
IPR006041	Pollen_Ole_e1_allergen
IPR006040	Allegen_Ole_e_I_CS

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005615	extracellular space
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi06G003550.1	Lsi06G003550.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006040	Allergen Ole e 1, conserved site	PROSITE	PS00925	OLEEI	coord: 31..40 scor
IPR006041	Pollen allergen Ole e 1 family	PANTHER	PTHR31614:SF2	F28N24.16 PROTEIN	coord: 1..162 score: 3.7
None	No IPR available	PANTHER	PTHR31614	FAMILY NOT NAMED	coord: 1..162 score: 3.7
None	No IPR available	PFAM	PF01190	Pollen_Ole_e_I	coord: 30..118 score: 2.7

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi06G003550	Csa1G597700	Cucumber (Chinese Long) v2	culsiB058
Lsi06G003550	CSPI01G28900	Wild cucumber (PI 183967)	cpilsiB059
Lsi06G003550	Cucsa.141950	Cucumber (Gy14) v1	cgylsiB230
Lsi06G003550	CmaCh10G007830	Cucurbita maxima (Rimu)	cmalsiB063
Lsi06G003550	CmaCh11G007490	Cucurbita maxima (Rimu)	cmalsiB128
Lsi06G003550	CmoCh11G007600	Cucurbita moschata (Rifu)	cmolsiB118
Lsi06G003550	CmoCh10G008100	Cucurbita moschata (Rifu)	cmolsiB050
Lsi06G003550	Cla011142	Watermelon (97103) v1	lsiwmB396
Lsi06G003550	MELO3C015539	Melon (DHL92) v3.5.1	lsimeB396
Lsi06G003550	ClCG03G014880	Watermelon (Charleston Gray)	lsiwcgB364
Lsi06G003550	MELO3C015539.2	Melon (DHL92) v3.6.1	lsimedB418
Lsi06G003550	Cla97C03G065110	Watermelon (97103) v2	lsiwmbB365
Lsi06G003550	Bhi02G001491	Wax gourd	lsiwgoB517
Lsi06G003550	Cp4.1LG04g11160	Cucurbita pepo (Zucchini)	cpelsiB563
Lsi06G003550	Cp4.1LG18g02800	Cucurbita pepo (Zucchini)	cpelsiB305
Lsi06G003550	CsGy1G028120	Cucumber (Gy14) v2	cgyblsiB055
Lsi06G003550	CsaV3_1G041280	Cucumber (Chinese Long) v3	cuclsiB068

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi06G003550	Bottle gourd (USVL1VR-Ls)	lsilsiB141
Lsi06G003550	Cucurbita maxima (Rimu)	cmalsiB445
Lsi06G003550	Wild cucumber (PI 183967)	cpilsiB299
Lsi06G003550	Cucumber (Chinese Long) v2	culsiB299
Lsi06G003550	Melon (DHL92) v3.5.1	lsimeB423
Lsi06G003550	Cucumber (Gy14) v2	cgyblsiB276
Lsi06G003550	Melon (DHL92) v3.6.1	lsimedB445
Lsi06G003550	Silver-seed gourd	carlsiB086
Lsi06G003550	Silver-seed gourd	carlsiB817
Lsi06G003550	Cucumber (Chinese Long) v3	cuclsiB319
Lsi06G003550	Wax gourd	lsiwgoB499