BLAST of Lsi06G001610 vs. Swiss-Prot
Match:
RGA3_SOLBU (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum GN=RGA3 PE=2 SV=2)
HSP 1 Score: 538.5 bits (1386), Expect = 1.7e-151
Identity = 362/1019 (35.53%), Postives = 570/1019 (55.94%), Query Frame = 1
Query: 1 MADSILFNLAGNIITKLGSFAIQ-ELGLLWGVHDELHKLKATVSAIDAVLLDAEEQQFKS 60
MA++ L L N+ +F IQ ELGL++G E KL + S I AVL DA+E+Q K
Sbjct: 1 MAEAFLQVLLDNL-----TFFIQGELGLVFGFEKEFKKLSSMFSMIQAVLEDAQEKQLKY 60
Query: 61 HAVKDWISKLKDAFYDIDDLMDEFSYESLR--RQVMTKHRPNTKQVRIFFSKSNQIAFLL 120
A+K+W+ KL A Y++DD++D+ E+ R + V+ ++ P T I F
Sbjct: 61 KAIKNWLQKLNVAAYEVDDILDDCKTEAARFKQAVLGRYHPRT------------ITFCY 120
Query: 121 KMGQKIKTVREKLDAIARDKAQLNLIENTREIRVDESTKLPETSSFILEGEVIGRDRDRK 180
K+G+++K + EKLDAIA ++ +L E ++ +T + E +V GR+++
Sbjct: 121 KVGKRMKEMMEKLDAIAEERRNFHLDERI----IERQAARRQTGFVLTEPKVYGREKEED 180
Query: 181 RIVDFLLDTNTEMDNVKVIAIIGMGGLGKTALAQSIYGDEKVKEHFELTMWVCISEEFDV 240
IV L++ + + V V+ I+GMGGLGKT LAQ ++ D+++ EHF L +WVC+S++FD
Sbjct: 181 EIVKILINNVSYSEEVPVLPILGMGGLGKTTLAQMVFNDQRITEHFNLKIWVCVSDDFDE 240
Query: 241 KVIVEKIIESLTKMRPEPNLQLNTLQSMLREKIDGKKYLLVMDDVWNVNHEKWISLKRYL 300
K +++ I+ES+ + + ++ L LQ L+E ++GK+Y LV+DDVWN + EKW +L+ L
Sbjct: 241 KRLIKAIVESI-EGKSLGDMDLAPLQKKLQELLNGKRYFLVLDDVWNEDQEKWDNLRAVL 300
Query: 301 MGGAKGSRILITTRSHQVAETSETVLFHDLKELDKDNSWELFRKMAFSNESEELENSKLV 360
GA G+ ILITTR ++ T+ + L L +++ W LF++ AF +++E + KL+
Sbjct: 301 KIGASGASILITTRLEKIGSIMGTLQLYQLSNLSQEDCWLLFKQRAFCHQTET--SPKLM 360
Query: 361 VIGKEIVAKLKGSPLAIRIIGRLLYSKRSEKDYWLSFKENELRTVMQKENEIQSILKISF 420
IGKEIV K G PLA + +G LL KR E + W +++E+ + Q EN + L++S+
Sbjct: 361 EIGKEIVKKCGGVPLAAKTLGGLLRFKREESE-WEHVRDSEIWNLPQDENSVLPALRLSY 420
Query: 421 NHLSSSLKQCFTYCALFPKDYEIQKDELIKQWMAQGFIQPHSKKAIEDVGDDYFKELLGR 480
+HL L+QCF YCA+FPKD +I+K+ LI WMA F+ +EDVG++ + EL R
Sbjct: 421 HHLPLDLRQCFAYCAVFPKDTKIEKEYLIALWMAHSFLLSKGNMELEDVGNEVWNELYLR 480
Query: 481 SFFQDIRKNKRGEIKEFKMHDFMHDLACSVGENDFVLVHDDTMPIDKGVRHVTFPFYLMK 540
SFFQ+I + K G+ FKMHD +HDLA S+ F V+ +++
Sbjct: 481 SFFQEI-EVKSGK-TYFKMHDLIHDLATSM----FSASASSRSIRQINVKDDEDMMFIVT 540
Query: 541 RWKLIAKSLIEAKNLRTLNTPHVHGIDLSNHLRLRTLNSEYFDSFPKCIGKMKHLRYVNL 600
+K + I + + +P + +S LR+ L++ F+ P +G + HLRY++L
Sbjct: 541 NYKDMMS--IGFSEVVSSYSPSLFKRFVS--LRVLNLSNSEFEQLPSSVGDLVHLRYLDL 600
Query: 601 SNISIHFLPKFFTKLYHLETLILRNCYLLRGLPGDIKNLINLRHLDIMHCNSNNWTHMPI 660
S I LPK KL +L+TL L NC L LP L +LR+L + HC T MP
Sbjct: 601 SGNKICSLPKRLCKLQNLQTLDLYNCQSLSCLPKQTSKLCSLRNLVLDHC---PLTSMPP 660
Query: 661 GLGSMTSLQTMNLFVLGKNKGSKLSELNGLNSLRGSLKIRHLQFCKSSDLENAKYLEEKF 720
+G +T L+T+ FV+G+ KG +L EL LN LRG++ I HL+ K+ L K
Sbjct: 661 RIGLLTCLKTLGYFVVGERKGYQLGELRNLN-LRGAISITHLERVKNDMEAKEANLSAKA 720
Query: 721 GIQKLELHWDRESKIDNALDDEDEKVLEGLKPHPNLHKIHIEGFKGVKLCNWLSFDSISS 780
+ L + WDR N + E+ KVLE LKPHPNL + I F G L +W++ + +
Sbjct: 721 NLHSLSMSWDR----PNRYESEEVKVLEALKPHPNLKYLEIIDFCGFCLPDWMNHSVLKN 780
Query: 781 LVTIKLRNCEKLQHLPRFDQFPFLKHLHLEELS-NIEYIDNNNFVSSSTNFFPSLEKLSI 840
+V+I + CE LP F + P L+ L L++ S +EY++++ F++ FPSL KL I
Sbjct: 781 VVSILISGCENCSCLPPFGELPCLESLELQDGSVEVEYVEDSGFLTRRR--FPSLRKLHI 840
Query: 841 ISMPNLKGWWKGEMVTTNIIVGEDSSS---TNVFRSLSSLSISEMDFEFLPHDLFCNMTH 900
NLKG + + ++ E S VF +LSS+ E+ E +
Sbjct: 841 GGFCNLKGLQRMKGAEQFPVLEEMKISDCPMFVFPTLSSVKKLEIWGE-------ADAGG 900
Query: 901 LKSL-HISHCKNLKMSSDHD-----DVIWKELESLHSLKLTFIPKLEYLPKGFQYMTAVE 960
L S+ ++S +LK+ S+H + ++K LE+L L ++F+ L+ LP + ++
Sbjct: 901 LSSISNLSTLTSLKIFSNHTVTSLLEEMFKNLENLIYLSVSFLENLKELPTSLASLNNLK 960
Query: 961 HLILADCPNLVCI--EGIEHLTSLSSLTIFNCPSLTSLPQEMGQLTSLSSLTIIDCPNL 1005
L + C L + EG+E L+SL+ L + +C L LP+ + LT+L+SL I CP L
Sbjct: 961 CLDIRYCYALESLPEEGLEGLSSLTELFVEHCNMLKCLPEGLQHLTTLTSLKIRGCPQL 967
BLAST of Lsi06G001610 vs. Swiss-Prot
Match:
RGA2_SOLBU (Disease resistance protein RGA2 OS=Solanum bulbocastanum GN=RGA2 PE=1 SV=1)
HSP 1 Score: 521.5 bits (1342), Expect = 2.2e-146
Identity = 352/995 (35.38%), Postives = 535/995 (53.77%), Query Frame = 1
Query: 13 IITKLGSFAIQELGLLWGVHDELHKLKATVSAIDAVLLDAEEQQFKSHAVKDWISKLKDA 72
++ L SF EL LL+G DE +L + S I AVL DA+E+Q + +++W+ KL A
Sbjct: 9 LLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNNKPLENWLQKLNAA 68
Query: 73 FYDIDDLMDEFSYESLRRQVMTKHRPNTKQVRIFFSKSNQIAFLLKMGQKIKTVREKLDA 132
Y++DD++DE+ ++ R R + K I F K+G+++ V +KL A
Sbjct: 69 TYEVDDILDEYKTKATRFSQSEYGRYHPKV----------IPFRHKVGKRMDQVMKKLKA 128
Query: 133 IARDKAQLNLIENTREIRVDESTKLPETSSFILEGEVIGRDRDRKRIVDFLLDTNTEMDN 192
IA ++ +L E V+ ET S + E +V GRD+++ IV L++ ++ +
Sbjct: 129 IAEERKNFHLHEKI----VERQAVRRETGSVLTEPQVYGRDKEKDEIVKILINNVSDAQH 188
Query: 193 VKVIAIIGMGGLGKTALAQSIYGDEKVKEHFELTMWVCISEEFDVKVIVEKIIESLTKMR 252
+ V+ I+GMGGLGKT LAQ ++ D++V EHF +W+C+SE+FD K +++ I+ES+
Sbjct: 189 LSVLPILGMGGLGKTTLAQMVFNDQRVTEHFHSKIWICVSEDFDEKRLIKAIVESIEGRP 248
Query: 253 PEPNLQLNTLQSMLREKIDGKKYLLVMDDVWNVNHEKWISLKRYLMGGAKGSRILITTRS 312
+ L LQ L+E ++GK+YLLV+DDVWN + +KW +L+ L GA G+ +L TTR
Sbjct: 249 LLGEMDLAPLQKKLQELLNGKRYLLVLDDVWNEDQQKWANLRAVLKVGASGASVLTTTRL 308
Query: 313 HQVAETSETVLFHDLKELDKDNSWELFRKMAFSNESEELENSKLVVIGKEIVAKLKGSPL 372
+V T+ ++L L +++ W LF + AF ++ E N LV IGKEIV K G PL
Sbjct: 309 EKVGSIMGTLQPYELSNLSQEDCWLLFMQRAFGHQEE--INPNLVAIGKEIVKKSGGVPL 368
Query: 373 AIRIIGRLLYSKRSEKDYWLSFKENELRTVMQKENEIQSILKISFNHLSSSLKQCFTYCA 432
A + +G +L KR E+ W +++ + + Q E+ I L++S++ L LKQCF YCA
Sbjct: 369 AAKTLGGILCFKREER-AWEHVRDSPIWNLPQDESSILPALRLSYHQLPLDLKQCFAYCA 428
Query: 433 LFPKDYEIQKDELIKQWMAQGFIQPHSKKAIEDVGDDYFKELLGRSFFQDIRKNKRGEIK 492
+FPKD +++K++LI WMA GF+ +EDVGD+ +KEL RSFFQ+I E+K
Sbjct: 429 VFPKDAKMEKEKLISLWMAHGFLLSKGNMELEDVGDEVWKELYLRSFFQEI------EVK 488
Query: 493 E----FKMHDFMHDLACSVGENDF-------VLVHDDTMPIDKGVRHVTFPFYLMKRWKL 552
+ FKMHD +HDLA S+ + + H T + G V F + L K
Sbjct: 489 DGKTYFKMHDLIHDLATSLFSANTSSSNIREINKHSYTHMMSIGFAEVVFFYTLPPLEKF 548
Query: 553 IAKSLIEAKNLRTLNTPHVHGIDLSNHLRLRTLNSEYFDSFPKCIGKMKHLRYVNLSNIS 612
I+ LR+ L F+ P IG + HLRY+NL
Sbjct: 549 IS-------------------------LRVLNLGDSTFNKLPSSIGDLVHLRYLNLYGSG 608
Query: 613 IHFLPKFFTKLYHLETLILRNCYLLRGLPGDIKNLINLRHLDIMHCNSNNWTHMPIGLGS 672
+ LPK KL +L+TL L+ C L LP + L +LR+L + S + T MP +GS
Sbjct: 609 MRSLPKQLCKLQNLQTLDLQYCTKLCCLPKETSKLGSLRNL--LLDGSQSLTCMPPRIGS 668
Query: 673 MTSLQTMNLFVLGKNKGSKLSELNGLNSLRGSLKIRHLQFCKSSDLENAKYLEEKFGIQK 732
+T L+T+ FV+G+ KG +L EL LN L GS+KI HL+ K+ L K +
Sbjct: 669 LTCLKTLGQFVVGRKKGYQLGELGNLN-LYGSIKISHLERVKNDKDAKEANLSAKGNLHS 728
Query: 733 LELHWDRESKIDNALDDEDEKVLEGLKPHPNLHKIHIEGFKGVKLCNWLSFDSISSLVTI 792
L + W+ + + E+ KVLE LKPH NL + I GF+G+ L W++ + ++V+I
Sbjct: 729 LSMSWNNFG--PHIYESEEVKVLEALKPHSNLTSLKIYGFRGIHLPEWMNHSVLKNIVSI 788
Query: 793 KLRNCEKLQHLPRFDQFPFLKHLHLE----ELSNIEYIDNNNFVSSSTNF-FPSLEKLSI 852
+ N LP F P L+ L L ++ +E +D + T FPSL KL I
Sbjct: 789 LISNFRNCSCLPPFGDLPCLESLELHWGSADVEYVEEVDIDVHSGFPTRIRFPSLRKLDI 848
Query: 853 ISMPNLKGWWKGE-----MVTTNIIVGEDSSST--NVFRSLSSLSI--SEMDFEFLPHDL 912
+LKG K E V +I+ E T + R+L+SL I +++ F P ++
Sbjct: 849 WDFGSLKGLLKKEGEEQFPVLEEMIIHECPFLTLSSNLRALTSLRICYNKVATSF-PEEM 908
Query: 913 FCNMTHLKSLHISHCKNLKMSSDHDDVIWKELESLHSLKLTFIPKLEYLP-KGFQYMTAV 972
F N+ +LK L IS C NLK L +L SLK+ LE LP +G + ++++
Sbjct: 909 FKNLANLKYLTISRCNNLK----ELPTSLASLNALKSLKIQLCCALESLPEEGLEGLSSL 945
Query: 973 EHLILADCPNLVCI-EGIEHLTSLSSLTIFNCPSL 981
L + C L C+ EG++HLT+L+SL I CP L
Sbjct: 969 TELFVEHCNMLKCLPEGLQHLTTLTSLKIRGCPQL 945
BLAST of Lsi06G001610 vs. Swiss-Prot
Match:
RGA4_SOLBU (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum GN=RGA4 PE=2 SV=1)
HSP 1 Score: 519.2 bits (1336), Expect = 1.1e-145
Identity = 354/1024 (34.57%), Postives = 548/1024 (53.52%), Query Frame = 1
Query: 13 IITKLGSFAIQELGLLWGVHDELHKLKATVSAIDAVLLDAEEQQFKSHAVKDWISKLKDA 72
++ L SF +L L++G E KL + S I AVL DA+E+Q K A+++W+ KL A
Sbjct: 9 LLENLTSFIGDKLVLIFGFEKECEKLSSVFSTIQAVLQDAQEKQLKDKAIENWLQKLNSA 68
Query: 73 FYDIDDLMDEFSYESLRRQVMTKHRPNTKQVRIFFSKSNQIAFLLKMGQKIKTVREKLDA 132
Y++DD++ E E++R + Q R+ F I F K+G+++K + EKLDA
Sbjct: 69 AYEVDDILGECKNEAIRFE----------QSRLGFYHPGIINFRHKIGRRMKEIMEKLDA 128
Query: 133 IARDKAQLNLIENTREIRVDESTKLPETSSFILEGEVIGRDRDRKRIVDFLLDTNTEMDN 192
I+ ++ + + +E E + +T+ ET + E +V GRD++ IV L++ +
Sbjct: 129 ISEERRKFHFLEKITERQAAAATR--ETGFVLTEPKVYGRDKEEDEIVKILINNVNVAEE 188
Query: 193 VKVIAIIGMGGLGKTALAQSIYGDEKVKEHFELTMWVCISEEFDVKVIVEKIIESLTKMR 252
+ V IIGMGGLGKT LAQ I+ DE+V +HF +WVC+S++FD K +++ II ++ +
Sbjct: 189 LPVFPIIGMGGLGKTTLAQMIFNDERVTKHFNPKIWVCVSDDFDEKRLIKTIIGNIERSS 248
Query: 253 PEPNLQLNTLQSMLREKIDGKKYLLVMDDVWNVNHEKWISLKRYLMGGAKGSRILITTRS 312
P L + Q L+E ++GK+YLLV+DDVWN + EKW L+ L GA+G+ IL TTR
Sbjct: 249 PHVE-DLASFQKKLQELLNGKRYLLVLDDVWNDDLEKWAKLRAVLTVGARGASILATTRL 308
Query: 313 HQVAETSETVLFHDLKELDKDNSWELFRKMAFSNESEELENSKLVVIGKEIVAKLKGSPL 372
+V T+ + L L +S LF + AF + E N LV IGKEIV K G PL
Sbjct: 309 EKVGSIMGTLQPYHLSNLSPHDSLLLFMQRAFGQQKEA--NPNLVAIGKEIVKKCGGVPL 368
Query: 373 AIRIIGRLLYSKRSEKDYWLSFKENELRTVMQKENEIQSILKISFNHLSSSLKQCFTYCA 432
A + +G LL KR E + W ++NE+ ++ Q E+ I L++S++HL L+QCF YCA
Sbjct: 369 AAKTLGGLLRFKREESE-WEHVRDNEIWSLPQDESSILPALRLSYHHLPLDLRQCFAYCA 428
Query: 433 LFPKDYEIQKDELIKQWMAQGFIQPHSKKAIEDVGDDYFKELLGRSFFQDIRKNKRGEIK 492
+FPKD ++ K+ LI WMA GF+ +EDVG++ + EL RSFFQ+I + K G
Sbjct: 429 VFPKDTKMIKENLITLWMAHGFLLSKGNLELEDVGNEVWNELYLRSFFQEI-EAKSGN-T 488
Query: 493 EFKMHDFMHDLACSVGENDFVLVHDDTMPIDKGVRHVTFPFYLMKRWKLIAKSLIEAKNL 552
FK+HD +HDLA S+ +R + Y K I + + +
Sbjct: 489 YFKIHDLIHDLATSL---------FSASASCGNIREINVKDY--KHTVSIGFAAVVSSYS 548
Query: 553 RTLNTPHVHGIDLSNHLRLRTLNSEY--FDSFPKCIGKMKHLRYVNLSNISIHFLPKFFT 612
+L L + LR LN Y + P IG + HLRY++LS + LP+
Sbjct: 549 PSL---------LKKFVSLRVLNLSYSKLEQLPSSIGDLLHLRYLDLSCNNFRSLPERLC 608
Query: 613 KLYHLETLILRNCYLLRGLPGDIKNLINLRHLDIMHCNSNNWTHMPIGLGSMTSLQTMNL 672
KL +L+TL + NCY L LP L +LRHL + C T P +G +T L+T+
Sbjct: 609 KLQNLQTLDVHNCYSLNCLPKQTSKLSSLRHLVVDGC---PLTSTPPRIGLLTCLKTLGF 668
Query: 673 FVLGKNKGSKLSELNGLNSLRGSLKIRHLQFCKSSDLENAKYLEEKFGIQKLELHWDRES 732
F++G KG +L EL LN L GS+ I HL+ K +D + L K +Q L + WD +
Sbjct: 669 FIVGSKKGYQLGELKNLN-LCGSISITHLERVK-NDTDAEANLSAKANLQSLSMSWDNDG 728
Query: 733 KIDNALDDEDEKVLEGLKPHPNLHKIHIEGFKGVKLCNWLSFDSISSLVTIKLRNCEKLQ 792
N + ++ KVLE LKPHPNL + I F G + +W++ + ++++++++C+
Sbjct: 729 --PNRYESKEVKVLEALKPHPNLKYLEIIAFGGFRFPSWINHSVLEKVISVRIKSCKNCL 788
Query: 793 HLPRFDQFPFLKHLHLEELS-NIEYIDNNNFVS--SSTNFFPSLEKLSIISMPNLKGWWK 852
LP F + P L++L L+ S +EY++ ++ S S+ FPSL+KL I +LKG K
Sbjct: 789 CLPPFGELPCLENLELQNGSAEVEYVEEDDVHSRFSTRRSFPSLKKLRIWFFRSLKGLMK 848
Query: 853 GEMVTTNIIVGEDSSSTNVFRSLSSLSISEMDFEFLPHDLFCNMTHLKSLHISHCKNLKM 912
E GE+ + EM + P +F ++ +K L + N +
Sbjct: 849 EE--------GEEKFP----------MLEEMAILYCPLFVFPTLSSVKKLEVHGNTNTRG 908
Query: 913 SSDHDDVIWKELESLHSLKLTFIPKLEYLPKG-FQYMTAVEHLILADCPNLVCI-EGIEH 972
S + L +L SL++ + LP+ F +T +E L D NL + +
Sbjct: 909 LSSISN-----LSTLTSLRIGANYRATSLPEEMFTSLTNLEFLSFFDFKNLKDLPTSLTS 964
Query: 973 LTSLSSLTIFNCPSLTSLPQE-MGQLTSLSSLTIIDCPNLTSLPQEMGQLTSLSSLGIFR 1029
L +L L I +C SL S P++ + LTSL+ L + C L LP+ + LT+L++LG+
Sbjct: 969 LNALKRLQIESCDSLESFPEQGLEGLTSLTQLFVKYCKMLKCLPEGLQHLTALTNLGVSG 964
BLAST of Lsi06G001610 vs. Swiss-Prot
Match:
RGA1_SOLBU (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum GN=RGA1 PE=2 SV=2)
HSP 1 Score: 500.4 bits (1287), Expect = 5.2e-140
Identity = 343/1000 (34.30%), Postives = 535/1000 (53.50%), Query Frame = 1
Query: 13 IITKLGSFAIQELGLLWGVHDELHKLKATVSAIDAVLLDAEEQQFKSHAVKDWISKLKDA 72
++ L SF EL LL+G DE +L + S I AVL DA+E+Q +++W+ KL A
Sbjct: 9 VLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNDKPLENWLQKLNAA 68
Query: 73 FYDIDDLMDEFSYESLRRQVMTKHRPNTKQVRIFFSKSNQIAFLLKMGQKIKTVREKLDA 132
Y++DD++DE+ ++ R R + K I F K+G+++ V +KL+A
Sbjct: 69 TYEVDDILDEYKTKATRFLQSEYGRYHPKV----------IPFRHKVGKRMDQVMKKLNA 128
Query: 133 IARDKAQLNLIENTREIRVDESTKLPETSSFILEGEVIGRDRDRKRIVDFLLDTNTEMDN 192
IA ++ + +L E ++ ET S + E +V GRD+++ IV L++T ++
Sbjct: 129 IAEERKKFHLQEKI----IERQAATRETGSVLTEPQVYGRDKEKDEIVKILINTASDAQK 188
Query: 193 VKVIAIIGMGGLGKTALAQSIYGDEKVKEHFELTMWVCISEEFDVKVIVEKIIESLTKMR 252
+ V+ I+GMGGLGKT L+Q ++ D++V E F +W+CIS++F+ K +++ I+ES+ + +
Sbjct: 189 LSVLPILGMGGLGKTTLSQMVFNDQRVTERFYPKIWICISDDFNEKRLIKAIVESI-EGK 248
Query: 253 PEPNLQLNTLQSMLREKIDGKKYLLVMDDVWNVNHEKWISLKRYLMGGAKGSRILITTRS 312
++ L LQ L+E ++GK+Y LV+DDVWN + KW +L+ L GA G+ +L TTR
Sbjct: 249 SLSDMDLAPLQKKLQELLNGKRYFLVLDDVWNEDQHKWANLRAVLKVGASGAFVLTTTRL 308
Query: 313 HQVAETSETVLFHDLKELDKDNSWELFRKMAFSNESEELENSKLVVIGKEIVAKLKGSPL 372
+V T+ ++L L ++ W LF + AF ++ E N L+ IGKEIV K G PL
Sbjct: 309 EKVGSIMGTLQPYELSNLSPEDCWFLFMQRAFGHQEE--INPNLMAIGKEIVKKCGGVPL 368
Query: 373 AIRIIGRLLYSKRSEKDYWLSFKENELRTVMQKENEIQSILKISFNHLSSSLKQCFTYCA 432
A + +G +L KR E++ W +++ + + Q E+ I L++S++HL L+QCF YCA
Sbjct: 369 AAKTLGGILRFKREERE-WEHVRDSPIWNLPQDESSILPALRLSYHHLPLDLRQCFVYCA 428
Query: 433 LFPKDYEIQKDELIKQWMAQGFIQPHSKKAIEDVGDDYFKELLGRSFFQDIRKNKRGEIK 492
+FPKD ++ K+ LI WMA GF+ +EDVG++ + EL RSFFQ+I
Sbjct: 429 VFPKDTKMAKENLIAFWMAHGFLLSKGNLELEDVGNEVWNELYLRSFFQEIEVESGKTY- 488
Query: 493 EFKMHDFMHDLACSVGENDFVLVHDDTMPIDKGVRHVTFPFYLMKRWKLIAKSLIEAKNL 552
FKMHD +HDLA S L +T + + + Y+M I + + +
Sbjct: 489 -FKMHDLIHDLATS-------LFSANTSSSNIREINANYDGYMMS----IGFAEVVSSYS 548
Query: 553 RTLNTPHVHGIDLSNHLRLRTLNSEYFDSFPKCIGKMKHLRYVNLS-NISIHFLPKFFTK 612
+L V LR+ L + + P IG + HLRY++LS N I LPK K
Sbjct: 549 PSLLQKFV-------SLRVLNLRNSNLNQLPSSIGDLVHLRYLDLSGNFRIRNLPKRLCK 608
Query: 613 LYHLETLILRNCYLLRGLPGDIKNLINLRHLDIMHCNSNNWTHMPIGLGSMTSLQTMNLF 672
L +L+TL L C L LP L +LR+L + C + T P +G +T L++++ F
Sbjct: 609 LQNLQTLDLHYCDSLSCLPKQTSKLGSLRNLLLDGC---SLTSTPPRIGLLTCLKSLSCF 668
Query: 673 VLGKNKGSKLSELNGLNSLRGSLKIRHLQFCKSSDLENAKYLEEKFGIQKLELHWDRESK 732
V+GK KG +L EL LN L GS+ I L K L K + L L WD + K
Sbjct: 669 VIGKRKGHQLGELKNLN-LYGSISITKLDRVKKDTDAKEANLSAKANLHSLCLSWDLDGK 728
Query: 733 IDNALDDEDEKVLEGLKPHPNLHKIHIEGFKGVKLCNWLSFDSISSLVTIKLRNCEKLQH 792
D +VLE LKPH NL + I GF G++L +W++ + ++V+I++R CE
Sbjct: 729 -----HRYDSEVLEALKPHSNLKYLEINGFGGIRLPDWMNQSVLKNVVSIRIRGCENCSC 788
Query: 793 LPRFDQFPFLKHLHLEELS-NIEYIDNNNFVSSSTNFFPSLEKLSIISMPNLKGWWKGEM 852
LP F + P L+ L L S ++EY+++N FPSL KL I NLKG K E
Sbjct: 789 LPPFGELPCLESLELHTGSADVEYVEDN----VHPGRFPSLRKLVIWDFSNLKGLLKMEG 848
Query: 853 VTTNIIVGE------DSSSTNVFRSLSSLSISEMDFEFLPHDLFCNMTHLKSLHISHCKN 912
++ E S+ +L + D L N+ L SL IS N
Sbjct: 849 EKQFPVLEEMTFYWCPMFVIPTLSSVKTLKVIVTDATVLRS--ISNLRALTSLDIS--DN 908
Query: 913 LKMSSDHDDVIWKELESLHSLKLTFIPKLEYLPKGFQYMTAVEHLILADCPNLVCI--EG 972
++ +S ++ ++K L +L LK++F L+ LP + A++ L C L + EG
Sbjct: 909 VEATSLPEE-MFKSLANLKYLKISFFRNLKELPTSLASLNALKSLKFEFCDALESLPEEG 952
Query: 973 IEHLTSLSSLTIFNCPSLTSLPQEMGQLTSLSSLTIIDCP 1003
++ LTSL+ L++ NC L LP+ + LT+L++LTI CP
Sbjct: 969 VKGLTSLTELSVSNCMMLKCLPEGLQHLTALTTLTITQCP 952
BLAST of Lsi06G001610 vs. Swiss-Prot
Match:
R13L1_ARATH (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana GN=RPPL1 PE=3 SV=1)
HSP 1 Score: 412.5 bits (1059), Expect = 1.4e-113
Identity = 328/1067 (30.74%), Postives = 523/1067 (49.02%), Query Frame = 1
Query: 35 LHKLKATVSAIDAVLLDAEEQQFKSHAVKDWISKLKDAFYDIDDLMDEFSYESLRRQVMT 94
L +L + I AVL+DAEE+Q + V+ W+++L+D Y +D +D+ + E+LR +
Sbjct: 39 LERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLNIGA 98
Query: 95 KHRPNTKQVRIFFSKSNQIAFLLKMGQKIKTVREK----LDAIARDKAQLNLIENTREIR 154
+ ++ ++R + + FL + ++T EK L+ +A + L L E T I
Sbjct: 99 ESS-SSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGLKELTAMI- 158
Query: 155 VDESTKLPETSSFILEGEVIGRDRDRKRIVDFLLDTNTEMDNVKVIAIIGMGGLGKTALA 214
+LP T+S + E EV GRD D+ I+ FL+ N + + + V+AI+G+GG+GKT L+
Sbjct: 159 --PKQRLP-TTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGVGKTTLS 218
Query: 215 QSIYGDEKVKEHFELTMWVCISEEFDVKVIVEKIIESLTKMRPEPNLQLNTLQSMLREKI 274
Q +Y D+ V+ +F +W +SEEFDV I +K+ ES+T RP L+ LQ L+E++
Sbjct: 219 QLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTS-RPCEFTDLDVLQVKLKERL 278
Query: 275 DGK--KYLLVMDDVWNVNHEKWISLKRYLMGGAKGSRILITTRSHQVAETSETVLFHDLK 334
G +LLV+DD+WN N W L++ + A+GS+IL+TTRS +VA V H+L+
Sbjct: 279 TGTGLPFLLVLDDLWNENFADWDLLRQPFIHAAQGSQILVTTRSQRVASIMCAVHVHNLQ 338
Query: 335 ELDKDNSWELFRKMAFSNESEELENSKLVVIGKEIVAKLKGSPLAIRIIGRLLYSKRSEK 394
L + W LF K F N+ E N ++ + + IV K +G PLA++ +G +L +
Sbjct: 339 PLSDGDCWSLFMKTVFGNQ-EPCLNREIGDLAERIVHKCRGLPLAVKTLGGVLRFEGKVI 398
Query: 395 DYWLSFKENELRTVMQKENEIQSILKISFNHLSSSLKQCFTYCALFPKDYEIQKDELIKQ 454
+ W + + + ++ + +L++S+ +L + LK+CF YC++FPK + +KD+++
Sbjct: 399 E-WERVLSSRIWDLPADKSNLLPVLRVSYYYLPAHLKRCFAYCSIFPKGHAFEKDKVVLL 458
Query: 455 WMAQGFIQP-HSKKAIEDVGDDYFKELLGRSFFQDIRKNKRGEIKEFKMHDFMHDLACSV 514
WMA+GF+Q S K +E++G++YF EL RS Q + + MHDF+++LA
Sbjct: 459 WMAEGFLQQTRSSKNLEELGNEYFSELESRSLLQKTKTR-------YIMHDFINELA-QF 518
Query: 515 GENDFVLVHDDTMPIDKGVRHVTFPFYLMKRWK--LIAKSLIEAKNLRTL------NTPH 574
+F +D + R + YL + + ++L E K LRT N+
Sbjct: 519 ASGEFSSKFEDGCKLQVSER-TRYLSYLRDNYAEPMEFEALREVKFLRTFLPLSLTNSSR 578
Query: 575 VHGID-------LSNHLRLRTLNSEYFDSF---PKCIGKMKHLRYVNLSNISIHFLPKFF 634
+D L RLR L+ ++ P + H R+++LS + LPK
Sbjct: 579 SCCLDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFFKNISHARFLDLSRTELEKLPKSL 638
Query: 635 TKLYHLETLILRNCYLLRGLPGDIKNLINLRHLDIMHCNSNNWTHMPIGLGSMTSLQTMN 694
+Y+L+TL+L C L+ LP DI NLINLR+LD++ MP G + SLQT+
Sbjct: 639 CYMYNLQTLLLSYCSSLKELPTDISNLINLRYLDLI---GTKLRQMPRRFGRLKSLQTLT 698
Query: 695 LFVLGKNKGSKLSELNGLNSLRGSLKIRHLQ-FCKSSDLENAKYLEEKFGIQKLELHW-- 754
F + + GS++SEL GL+ L G LKI LQ +D A L K +++++ W
Sbjct: 699 TFFVSASDGSRISELGGLHDLHGKLKIVELQRVVDVADAAEAN-LNSKKHLREIDFVWRT 758
Query: 755 ---DRESKIDNALDDEDEKVLEGLKPHPNLHKIHIEGFKGVKLCNWLSFDSISSLVTIKL 814
E+ + + +V E L+PH ++ K+ IE +KG + +WLS S S +V I+L
Sbjct: 759 GSSSSENNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYKGRRFPDWLSDPSFSRIVCIRL 818
Query: 815 RNCEKLQHLPRFDQFPFLKHLHLEELSNIE------YIDNNNFVSSSTNFFPSLEKLSII 874
R C+ LP Q P LK LH+ + ++ Y + F SLE L
Sbjct: 819 RECQYCTSLPSLGQLPCLKELHISGMVGLQSIGRKFYFSDQQLRDQDQQPFRSLETLRFD 878
Query: 875 SMPNLKGWWKGEMVTTNIIVGEDSSSTNVFRSLSSLSISEMDFEFLPHDLFCNMTHLKSL 934
++P+ + W + + G+ +F SL L I + L L + L SL
Sbjct: 879 NLPDWQEW-----LDVRVTRGD------LFPSLKKLFI--LRCPELTGTLPTFLPSLISL 938
Query: 935 HISHCKNLKMSSDHDDVIWKELESLHSLKLTFIPKLEYLPKGFQYMTAVEHLILADCPNL 994
HI C L DH + ++ L++L I + C L
Sbjct: 939 HIYKCGLLDFQPDHHEYSYRNLQTLS--------------------------IKSSCDTL 998
Query: 995 VCIEGIEHLTSLSSLTIFNCPSLTSLPQEMGQL---TSLSSLTIIDCPNLTSLPQEMGQL 1054
V + H +L L + C SL SL L +L +L I DC NL LP+
Sbjct: 999 VKFP-LNHFANLDKLEVDQCTSLYSLELSNEHLRGPNALRNLRINDCQNLQLLPK----- 1016
Query: 1055 TSLSSLGIFRCPNLTSLPQEMDQVTSLSSLTIDDITNLISLPQEMRQ 1062
L +LPQ + QVT ITN L Q M Q
Sbjct: 1059 -------------LNALPQNL-QVT---------ITNCRYLRQPMEQ 1016
BLAST of Lsi06G001610 vs. TrEMBL
Match:
A0A0A0M1L7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G572370 PE=3 SV=1)
HSP 1 Score: 798.5 bits (2061), Expect = 1.0e-227
Identity = 440/686 (64.14%), Postives = 505/686 (73.62%), Query Frame = 1
Query: 351 LENSKLVVIGKEIVAKLKGSPLAIRIIGRLLYSKRSEKDYWLSFKENELRTVMQKENEIQ 410
LENSKLV IGKEIVAKLKGSPLAIR+IG LYSK+SEKD WLSFKENEL T+MQ++NEIQ
Sbjct: 2 LENSKLVGIGKEIVAKLKGSPLAIRVIGSYLYSKKSEKD-WLSFKENELDTIMQQKNEIQ 61
Query: 411 SILKISFNHLSSSLKQCFTYCALFPKDYEIQKDELIKQWMAQGFIQPHSKKAIEDVGDDY 470
SILKISFNHLSSSLKQCFTYCALF KD+EI KD+LIKQWM +GFIQPH+KKA+EDVGD+Y
Sbjct: 62 SILKISFNHLSSSLKQCFTYCALFLKDFEIDKDDLIKQWMGEGFIQPHNKKAMEDVGDEY 121
Query: 471 FKELLGRSFFQDIRKNKRGEIKEFKMHDFMHDLACSVGENDFVLVHDDTMPIDKGVRHVT 530
FKELLGRSFFQDI KN+ GEI +FKMHDFMHDLAC VGEND+V DDT IDK RH++
Sbjct: 122 FKELLGRSFFQDISKNQLGEIMKFKMHDFMHDLACFVGENDYVFATDDTKFIDKRTRHLS 181
Query: 531 F-PFYLMKRWKLIAKSLIEAKNLRTLNTP-HVHG-----IDLSNHLRLRTLNSEYFDSFP 590
PF RW++I +SLI AKNLRTLN H + ID SNHLRLRTLN + P
Sbjct: 182 ISPFISKTRWEVIKESLIAAKNLRTLNYACHNYDGDEIEIDFSNHLRLRTLNLIFSTHVP 241
Query: 591 KCIGKMKHLRYVNLSNISIHFLPKFFTKLYHLETLILRNCYLLRGLPGDIKNLINLRHLD 650
KCIGKMKHLRY+N + FLPK TKLYHLETLI R C+ LR LP DI NLINLRHL
Sbjct: 242 KCIGKMKHLRYINFTRCYFDFLPKVVTKLYHLETLIFRECFKLRELPSDITNLINLRHLG 301
Query: 651 IMHCNSNNWTHMPIGLGSMTSLQTMNLFVLGKNKGSKLSELNGLNSLRGSLKIRHLQFCK 710
I + ++MP G+GSMT+LQT+NLF+LG+N+G +LSELNGL +LRGSL I+ LQFCK
Sbjct: 302 I-NSLIEGLSYMPKGMGSMTTLQTLNLFILGENEGGELSELNGLINLRGSLSIQQLQFCK 361
Query: 711 SSDLENAKYLEEKFGIQKLELHWDR-ESKIDNALDDEDEKVLEGLKPHPNLHKIHIEGFK 770
+E+AK+LEEK GIQKL+L+W E K + +DDEDEKVLE LKPHPNL KI I G+
Sbjct: 362 PIGIESAKHLEEKSGIQKLKLYWYHLERKYE--IDDEDEKVLECLKPHPNLQKIVINGYG 421
Query: 771 GVKLCNWLSFDSISSLVTIKLRNCEKLQHLPRFDQFPFLKHLHLEELSNIEYIDNNNFVS 830
GVKLCNW SFD I +LV I L NC KLQ LPRFDQFPFLKHL L+ L N+E+IDNN+ VS
Sbjct: 422 GVKLCNWFSFDYIVNLVIIDLFNCNKLQQLPRFDQFPFLKHLKLQYLPNVEFIDNNDSVS 481
Query: 831 SS-TNFFPSLEKLSIISMPNLKGWWKG------------------------------EMV 890
SS T FFPSLEKL I +P LK WWK EM
Sbjct: 482 SSLTTFFPSLEKLRIFRLPKLKEWWKRKLIDQTIPQHRRLESLNISGVSLQVFELVMEMA 541
Query: 891 TTNIIVG-EDSSSTNVFRSLSSLSISEMDFEFLP-HDLFCNMTHLKSLHISHCKNLKMSS 950
TTNIIVG +DSSS+ SLS LSI ++DFEFL HDLF NMTHLKSL I +CKN+KMSS
Sbjct: 542 TTNIIVGSQDSSSSTTSISLSFLSIEDIDFEFLQFHDLFSNMTHLKSLWIINCKNIKMSS 601
Query: 951 DHDDVIWKELESLHSLKLTFIPKLEYLPKGFQYMTAVEHLILADCPNLVCIEGIEHL-TS 995
D V WK L SL L L+ IP LEYLPK Q +T ++ L + +CPNLV IE I HL TS
Sbjct: 602 SLDAVTWKGLGSLRELMLSSIPDLEYLPKSLQCVTTLQSLQIYNCPNLVSIESIRHLTTS 661
BLAST of Lsi06G001610 vs. TrEMBL
Match:
A0A0A0KIT2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G375730 PE=3 SV=1)
HSP 1 Score: 752.7 bits (1942), Expect = 6.5e-214
Identity = 442/782 (56.52%), Postives = 530/782 (67.77%), Query Frame = 1
Query: 323 LFHDLKELDKDNSWELFRKMAFSNESEEL--ENSKLVVIG------------KEIVAKLK 382
L+ + +E+ D S + +F E E + ++ K +I EIV KLK
Sbjct: 143 LYENTREIQDDESTKRLETTSFIREGEIIGRDDDKKSIIHYLLDTNIHEDSVAEIVTKLK 202
Query: 383 GSPLAIRIIGRLLYSKRSEKDYWLSFKENELRTVMQKENEIQSILKISFNHLSSSLKQCF 442
GSPLAIR+IG LYSK+SEKD WLSFK++EL T+MQ+ENEIQSILKISFNHLSSSLK CF
Sbjct: 203 GSPLAIRVIGSYLYSKKSEKD-WLSFKDHELDTIMQQENEIQSILKISFNHLSSSLKHCF 262
Query: 443 TYCALFPKDY--EIQKDELIKQWMAQGFIQPHSKKAIEDVGDDYFKELLGRSFFQDIRKN 502
TYCALF KDY EI+K++LIKQWMAQGFIQPH+KKA+EDVGDDYF+ELLGRSFFQDIRKN
Sbjct: 263 TYCALFSKDYHHEIRKNDLIKQWMAQGFIQPHNKKAMEDVGDDYFEELLGRSFFQDIRKN 322
Query: 503 KRGEIKEFKMHDFMHDLACSVGENDFVLVHDDTMPIDKGVRHVTFP-FYLMKRWKLIAKS 562
K GEI +FKMHD +HDLACSV END VL +DDT IDK RHV+ F M RWKLI KS
Sbjct: 323 KWGEINKFKMHDIIHDLACSVVENDCVLANDDTKSIDKRTRHVSISAFNSMTRWKLITKS 382
Query: 563 LIEAKNLRTLNTPHVHGIDLSNHLRLRTLNSEYFDSFPKCIGKMKHLRYVNLSNISIHFL 622
LIEAKNLRTLN H IDLSNHLRLRTLN E F PKCIGKMKHLRY+N++ I FL
Sbjct: 383 LIEAKNLRTLNYARRHHIDLSNHLRLRTLNLE-FHFVPKCIGKMKHLRYINITYCYIDFL 442
Query: 623 PKFFTKLYHLETLILRNCYLLRGLPGDIKNLINLRHLDIMHCNSNNWTHMPIGLGSMTSL 682
PK TKLYHLETLI+R C LR L DIKNLINLRHLDI + W++MP G+GSMT+L
Sbjct: 443 PKAVTKLYHLETLIIRGCLELRELSSDIKNLINLRHLDIKDFK-HVWSYMPKGMGSMTTL 502
Query: 683 QTMNLFVLGKNKGSKLSELNGLNSLRGSLKIRHLQFCKSSDLENAKYLEEKFGIQKLELH 742
QTMNLF+LG+NKG +LSELNGL +LRGSL I+ LQFCK LEN KYLEEK IQKLELH
Sbjct: 503 QTMNLFILGENKGGELSELNGLVNLRGSLSIQQLQFCKPIGLENVKYLEEKSRIQKLELH 562
Query: 743 W---DRESKIDNALDDEDEKVLEGLKPHPNLHKIHIEGFKGVKLCNWLSFDSISSLVTIK 802
W RESKID DEDE+VLE LKPH NL KI IEG++G+KLCNW SFDSI +LV IK
Sbjct: 563 WKTYQRESKID----DEDERVLESLKPHSNLQKIRIEGYRGLKLCNWFSFDSIVNLVFIK 622
Query: 803 LRNCEKLQHLPRFDQFPFLKHLHLEELSNIEYIDNNNFVSSS-TNFFPSLEKLSIISMPN 862
L NCEKLQ LPRFD+FPFLKHLHLE+L +IEYI NN+VSSS T FFPSLE LSII +PN
Sbjct: 623 LFNCEKLQQLPRFDRFPFLKHLHLEDLPSIEYIAINNYVSSSMTTFFPSLENLSIIKLPN 682
Query: 863 LKGWWKGEMVTTNIIVGEDSSSTNVFRSLSSLSI---------------SEMDFEFLPHD 922
LK WWKGE + N +S + R LS L I +D +
Sbjct: 683 LKEWWKGESIDQN------TSFPTILRHLSQLKIHYCRQLASIPQHGPLQSLDIRDISLQ 742
Query: 923 LFCNMTHLKSLHISHCKNLKMSSDHDDVIWKELESLHSLKLTFIPKLEYLPKG-FQYMTA 982
LF + + + +I ++ SS +D+ + SL K+ + E+LP F +T
Sbjct: 743 LFELVIKMTATNIIVGED--SSSSANDMFIRSSSSLKIWKIDW----EFLPNDLFSNVTH 802
Query: 983 VEHLILADCPNLVCIEGIEH-----LTSLSSLTIFNCPSLTSLPQEMGQLTSLSSLTIID 1042
++ L++ C NL ++ L SL +L + P L LP+ L +L L ++
Sbjct: 803 LQSLVIGRCFNLKMSFDDDNVRWKELGSLRTLRLCFIPKLEYLPKGFQYLKALEHLELLW 862
Query: 1043 CPNLTSLPQEMGQLTSLSSLGIFRCPNLTSLPQEMDQVTSLSSLTIDDITNLISLPQEMR 1063
C NL + + LTSLS L I CPNLTSLP+ M Q+ SL+ L IDD NL +LP+ +
Sbjct: 863 CENLACI-LGIEHLTSLSRLEISNCPNLTSLPEGMTQLISLTCLIIDDCPNLSTLPEGLH 904
BLAST of Lsi06G001610 vs. TrEMBL
Match:
A0A0D2NDD9_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_005G155200 PE=3 SV=1)
HSP 1 Score: 743.8 bits (1919), Expect = 3.0e-211
Identity = 467/1160 (40.26%), Postives = 682/1160 (58.79%), Query Frame = 1
Query: 1 MADSILFNLAGNIITKLGSFAIQELGLLWGVHDELHKLKATVSAIDAVLLDAEEQQFKSH 60
MA++ LFN+A I+ K+G+ A+QE+GL+WGV ++L KLK TVS I AVLLDAE+Q KSH
Sbjct: 1 MAEAGLFNIADGILGKIGNLALQEIGLVWGVKEQLEKLKNTVSTIKAVLLDAEDQHAKSH 60
Query: 61 AVKDWISKLKDAFYDIDDLMDEFSYESLRRQVMTKHRPNTKQVRIFFSKSNQIAFLLKMG 120
V+DW+ KLKDA YD DDL+D FS L+RQ KQV FSK +Q+ + LK+
Sbjct: 61 EVRDWLGKLKDAVYDTDDLLDNFSTHVLKRQ----QGKRGKQVSFLFSKVSQVTYNLKIS 120
Query: 121 QKIKTVREKLDAIARDKAQLNLIENTREIRVDESTKLPETSSFILEGEVIGRDRDRKRIV 180
+I+ +REKLDAIA DK + + + + + +T SF+ + +V+GR D+ I+
Sbjct: 121 HQIEAIREKLDAIAADKIKYHFTDRSPISIPLVKVERRQTHSFVRKEDVVGRQGDKDAIM 180
Query: 181 DFLLDTNTEMDNVKVIAIIGMGGLGKTALAQSIYGDEKVKEHFELTMWVCISEEFDVKVI 240
LL++N +DNV VI I+G+GG GKT LAQ Y DE+ +HFEL MWVC+S+ FDVK+I
Sbjct: 181 KRLLESNG-VDNVSVIPIVGIGGQGKTTLAQLAYNDERTVKHFELRMWVCVSDVFDVKMI 240
Query: 241 VEKIIESLTKMRPEPNLQLNTLQSMLREKIDGKKYLLVMDDVWNVNHEKWISLKRYLMGG 300
VEKI+ES T + E +L++++LQ+ LR++IDG+KYLLV+DD+WN + E+W++L LM G
Sbjct: 241 VEKILESATSSKYE-SLEMDSLQTHLRKRIDGRKYLLVLDDMWNDSRERWLNLADLLMNG 300
Query: 301 AKGSRILITTRSHQVAETSETVLFHDLKELDKDNSWELFRKMAFSNESEELENSKLVVIG 360
A+GS+I++TTR+ VA + T + L+ L +D SW LF K+AF ES+E +S+LV IG
Sbjct: 301 ARGSKIIVTTRAQLVASITGTSQPYLLEGLPEDMSWSLFEKVAFK-ESKETNDSRLVAIG 360
Query: 361 KEIVAKLKGSPLAIRIIGRLLYSKRSEKDYWLSFKENELRTVMQKENEIQSILKISFNHL 420
K+IV K G+PL IR IG +LY+K +E + WLS KE +L + + E+++ S+LK+S+ L
Sbjct: 361 KDIVKKCAGNPLVIRTIGGVLYTKDTETE-WLSLKERQLSMLTRNEDDVLSVLKLSYEQL 420
Query: 421 SSSLKQCFTYCALFPKDYEIQKDELIKQWMAQGFIQP-HSKKAIEDVGDDYFKELLGRSF 480
S LKQCF YC+LFPKDYEI K LI W+A+GFIQP + +E++G YF +LL RSF
Sbjct: 421 PSYLKQCFAYCSLFPKDYEINKQMLISLWIAEGFIQPLQGIQCLEELGGQYFMDLLRRSF 480
Query: 481 FQDIRKNKRGEIKEFKMHDFMHDLACSVGENDFVLVHDDTMPIDKGVRHVTFPFYLMKRW 540
FQD+ ++ G + K+HD MHDLA + +D +V D I + HV+ L W
Sbjct: 481 FQDVEYDEWGNVISCKLHDLMHDLAQLIAGSDCSMVDLDCENISERTHHVSLSAELDSSW 540
Query: 541 KLIAKSLIEAKNLRTLNTP----HVHGIDLSNH---------LRLRTLNSEYFDSFPKCI 600
K I +L+ A +RT P H +D +H +R+ L++ P+ I
Sbjct: 541 K-IPTTLLNANKIRTFLLPMQPIHRVVLDKVDHEAIISSFRLMRVLDLHNTGLHILPRII 600
Query: 601 GKMKHLRYVNLS-NISIHFLPKFFTKLYHLETLILRNCYLLRGLPGDIKNLINLRHLDIM 660
GK+KHLRY++LS N I LP T+L +L+TL + +C L LP + N+I+L+HL+
Sbjct: 601 GKLKHLRYLDLSKNEVIRKLPSSITELLNLQTLKIYSCKRLEQLPRKLSNMISLKHLETG 660
Query: 661 HCNSNNWTHMPIGLGSMTSLQTMNLFVLGKNK----GSKLSELNGLNSLRGSLKIRHLQF 720
C THMP G+G +TSLQT+ FV+G + L EL LN LRG L I L+
Sbjct: 661 QCTG--LTHMPSGIGQLTSLQTLTRFVVGMSSFEMASGGLRELKDLNELRGELMIAKLEN 720
Query: 721 CKS--SDLENAKYLEEKFGIQKLELHWDRESKIDNALD-DEDEKVLEGLKPHPNLHKIHI 780
++ ++ E A L+EK ++ L L W RE ++N + +EDE +LEGL+PH NL + HI
Sbjct: 721 LRNVAAECEEAN-LKEKQHLEVLTLDWSRE--VNNHVSFEEDEALLEGLQPHSNLQEFHI 780
Query: 781 EGFKGVKLCNWLSFDS---ISSLVTIKLRNCEKLQHLPRFDQFPFLKHLHLEELSNIEYI 840
G++ + W+SFD + +L+ I + NC K HLP F Q P LK L LE ++ +EYI
Sbjct: 781 YGYRAERFPKWMSFDMALLLPNLLEITIWNCIKCIHLPLFSQLPKLKVLRLEVITAVEYI 840
Query: 841 DNNNFVSSST---------------NFFPSLEKLSIISMPNLKGWWKGEMVTTNIIVGED 900
+++ SSS+ FFP LE+L + NLKGWW+ TN G
Sbjct: 841 EDSRAESSSSLSFKGNPMNRGREGKEFFPCLEELVFFDLRNLKGWWREAPPVTNNNHGAA 900
Query: 901 SSSTNVFRSLSSLSISEMDFEFLPHDLFCNMTHLKSLHISHCKNLKMSSDHDDVIWKELE 960
+S + S+ +S L + N+TH+ LH + L++ + ++ +
Sbjct: 901 ASPQRPLQKKESM-VSFPCLSKLKIGICTNLTHM-PLH-PFLEELELKNTPARLLQQSAM 960
Query: 961 SLHSLKLT---FIPKLEYLP-KGFQYMTA-----------VEHLILADCPNLVCI--EGI 1020
L ++ KL+ + G + + ++HL + +CP+LVC+ EG+
Sbjct: 961 VAAGANLVYPLYLSKLKVMHIDGIIDLVSFPEKGIHHLTSLQHLSIENCPDLVCLTEEGL 1020
Query: 1021 EHLTSLSSLTIFNCPSLTSLPQEMGQLTSLSSLTIIDCPNL---TSLPQEMGQLTSLSSL 1080
+ LTSL I C L SL + LT+L L I +C L L + + +L L +L
Sbjct: 1021 KSLTSLRFFNIRCCEILKSLFKGFKHLTALEELEIKECRELDLSKDLEENVMELQCLRTL 1080
Query: 1081 GIFRCPNLTSLPQEMDQVTSLSSLTIDDITNLISLPQEMRQLTSLSSLTIIDCPNLTSLP 1101
I P L+SLP + VT+L L I +NL +LP+ + LTSL ++DCP L S P
Sbjct: 1081 RIEDMPKLSSLPDGLQHVTTLKDLQISSCSNLKTLPEWIGNLTSLQRFEVLDCPQLASFP 1140
BLAST of Lsi06G001610 vs. TrEMBL
Match:
U5FEY9_POPTR (Uncharacterized protein (Fragment) OS=Populus trichocarpa GN=POPTR_0019s10290g PE=4 SV=1)
HSP 1 Score: 743.4 bits (1918), Expect = 4.0e-211
Identity = 467/1155 (40.43%), Postives = 674/1155 (58.35%), Query Frame = 1
Query: 1 MADSILFNLAGNIITKLGSFAIQELGLLWGVHDELHKLKATVSAIDAVLLDAEEQQFKSH 60
M +++L ++ I+ KLG+ A+QE+GL+WGV DEL KL+ T S I A+ LDAEEQQ KSH
Sbjct: 1 MTEAVLVSITDRILGKLGNLALQEIGLIWGVKDELEKLRNTASVIKAIFLDAEEQQTKSH 60
Query: 61 AVKDWISKLKDAFYDIDDLMDEFSYESLRRQVMTKHRPNTKQVRIFFSKSNQIAFLLKMG 120
V+DW+ KLKDA YD DDL+D+FS E L++Q+M + + +V FFSK + A+ M
Sbjct: 61 EVRDWLQKLKDAIYDADDLLDDFSTEMLQQQLMMQDQ-KAIEVCAFFSKIKKTAYGFSMS 120
Query: 121 QKIKTVREKLDAIARDKAQLNLIENTREIRVDESTKLPETSSFILEGEVIGRDRDRKRIV 180
KIK +RE+L+ IA D+++ +L ++ R++ + +T SF+ EV+GR+ D+ IV
Sbjct: 121 CKIKAIRERLNDIASDRSKFHLTDHPRQMP-SVIAEREQTHSFVCVEEVVGREDDKLAIV 180
Query: 181 DFLLDTNTEMDNVKVIAIIGMGGLGKTALAQSIYGDEKVKEHFELTMWVCISEEFDVKVI 240
+ LL +NTE +NV VI ++G+GGLGKT L Q +Y EK++ HFEL +WVC+S+ FDVK+I
Sbjct: 181 ELLLHSNTE-ENVSVIPVVGIGGLGKTTLVQLVYNSEKIRRHFELRIWVCVSDVFDVKLI 240
Query: 241 VEKIIESLTKMRPEPNLQLNTLQSMLREKIDGKKYLLVMDDVWNVNHEKWISLKRYLMGG 300
V+KI+ES T + + L++++L + L ++IDGKK+LL++DDVWN N E+W+ L+ LMGG
Sbjct: 241 VQKILESATNTKCD-GLEMDSLLTRLGKEIDGKKFLLILDDVWNDNRERWLKLRDLLMGG 300
Query: 301 AKGSRILITTRSHQVAETSETVLFHDLKELDKDNSWELFRKMAFSNESEELENSKLVVIG 360
A+GS++++TTR+ +A + T + L+ L +D SW LF K+AF + ++ EN++LV IG
Sbjct: 301 ARGSKVVVTTRTQLIATITGTAKPYFLRSLSEDESWSLFEKLAFK-QGKDFENTRLVAIG 360
Query: 361 KEIVAKLKGSPLAIRIIGRLLYSKRSEKDYWLSFKENELRTVMQKENEIQSILKISFNHL 420
KE+V K G PLAIR +G LLY K +E + WLSFK+ +L + Q EN+I ILK+S+ L
Sbjct: 361 KEVVKKCAGVPLAIRTMGSLLYCKDTETE-WLSFKDRDLSMIPQNENDILPILKLSYELL 420
Query: 421 SSSLKQCFTYCALFPKDYEIQKDELIKQWMAQGFIQP-HSKKAIEDVGDDYFKELLGRSF 480
LK CF YC+LFPKDYEI K LIK WMAQGF+QP + E+VG F +L RSF
Sbjct: 421 PPCLKNCFAYCSLFPKDYEINKQTLIKLWMAQGFLQPADGMQHPEEVGHQCFMDLARRSF 480
Query: 481 FQDIRKNKRGEIKEFKMHDFMHDLACSVGENDFVLVHDDTMPIDKGVRHVTFPFYLMKRW 540
FQD+ + G++ MHD MHDLA VG ++ V + I + +RHV+ F L
Sbjct: 481 FQDLEYGEWGDVVSCGMHDLMHDLALLVGGSESSTVDSNAENISERIRHVSLDFELDSSQ 540
Query: 541 KLIAKSLIEAKNLRTL-------------NTPHVHGIDLSNHLRLRTLNSEYFDSFPKCI 600
K I SL +A +RT PH I LR ++ D P I
Sbjct: 541 K-IPPSLFKANKIRTFVLPVQPVYRKILNQAPHDTIISSFRCLRALDFHNTGVDIVPSSI 600
Query: 601 GKMKHLRYVNLS-NISIHFLPKFFTKLYHLETLILRNCYLLRGLPGDIKNLINLRHLDIM 660
K+KHLRY++LS N + LP+ +L +L+TL L +C L LP I +I+LRHL+I
Sbjct: 601 SKLKHLRYLDLSKNEDLKRLPRCIARLKNLQTLKLSSCKRLEALPRHISKMISLRHLEID 660
Query: 661 HCNSNNWTHMPIGLGSMTSLQTMNLFVLGKNKGS-----KLSELNGLNSLRGSLKIRHLQ 720
C THMP GLG +T+LQT+ FV+GK S +L ELNGLN LRG KI L+
Sbjct: 661 QCTG--LTHMPNGLGQLTALQTLTQFVVGKYGSSPDLSARLRELNGLNDLRGEFKISKLE 720
Query: 721 FCKSSDLENAKY-LEEKFGIQKLELHWDRESKIDNALDDEDEKVLEGLKPHPNLHKIHIE 780
K S E+ + L+ K ++ L L W R D +D EDE +LE +PH NL + HI
Sbjct: 721 KLKVSATESREANLKGKENLEVLRLEWTRGVNDDRVID-EDEVLLESFQPHSNLKEFHIY 780
Query: 781 GFKGVKLCNWLSFD---SISSLVTIKLRNCEKLQHLPRFDQFPFLKHLHLEELSNIEYID 840
G++ K +W+ + + +L I + C + LP F Q P LK L LEE++ +EYI+
Sbjct: 781 GYRAGKFPSWMVLNLSLLLPNLQEIIIWRCYRCLELPMFSQLPMLKVLKLEEVTALEYIE 840
Query: 841 NNNFVSSSTN--------FFPSLEKLSIISMPNLKGWWKGEM-VTTNIIVGEDSSSTNVF 900
N++ SSS FFPSL++L + + N KGWW+ E+ V N ++++
Sbjct: 841 NSSNGSSSLGKREEKSALFFPSLQELRLFDLRNFKGWWREEVSVVNNDEATVETTTETAG 900
Query: 901 RSLSSLSISEMDFEFLPHDLFCNMTHLKSLHISHCKNLKMSSDH---DDVIWKELES-LH 960
SL S++ E + + L L I HC NL H ++V +K++ + L
Sbjct: 901 ISLPSVAACEKQQPLQQQLVLPSFPCLSKLTIGHCPNLSNLPLHPFLNEVEFKDVNAGLV 960
Query: 961 SLKLTFIPKLE---------YLPKGFQYMTAVEHLILADCPNLVCIE--GIEHLTSLSSL 1020
+ + +E LP F ++HL + +LV I G+++LT L L
Sbjct: 961 QWSMVGLASIEGSSASGRNTSLP-SFPSTLKLKHLCMDSVMDLVSISELGLQNLTYLEHL 1020
Query: 1021 TIFNCPSLTSLPQE-MGQLTSLSSLTIIDCPNLTSLPQEMGQLTSLSSLGIFRCPNLTSL 1080
TI NCP+L+SLP+E + L SL L+I C +LTSL + LTSL L I C L
Sbjct: 1021 TIENCPNLSSLPEESLRGLRSLRFLSIRGCGSLTSLFLGLQYLTSLEELEIEECRALDMS 1080
Query: 1081 PQEMDQ------VTSLSSLTIDDITNLISLPQEMRQLTSLSSLTIIDCPNLTSLPQEMGQ 1101
+ + + SL L I + L S+P + ++TSL L I C L +LP+ +
Sbjct: 1081 DCDEENSLQFRGMKSLRRLKIGYMPQLESIPDGIHEVTSLQDLKIEGCVGLKTLPEWIHD 1140
BLAST of Lsi06G001610 vs. TrEMBL
Match:
A0A061EA34_THECC (LRR and NB-ARC domains-containing disease resistance protein, putative OS=Theobroma cacao GN=TCM_011164 PE=3 SV=1)
HSP 1 Score: 739.2 bits (1907), Expect = 7.5e-210
Identity = 476/1160 (41.03%), Postives = 673/1160 (58.02%), Query Frame = 1
Query: 1 MADSILFNLAGNIITKLGSFAIQELGLLWGVHDELHKLKATVSAIDAVLLDAEEQQFKSH 60
MA++ LFN+A I+ KLG+ +QE+GL+WGV +EL KLK TVS I AVLLDAEEQ KSH
Sbjct: 8 MAEAFLFNIADGILGKLGNLTLQEMGLIWGVKEELEKLKNTVSTIKAVLLDAEEQHAKSH 67
Query: 61 AVKDWISKLKDAFYDIDDLMDEFSYESLRRQVMTKHRPNTKQVRIFFSKSNQIAFLLKMG 120
V DW+ KLKDA YD DDL+D+FS L+RQVM + + KQV FSK+NQ+A+ LK+G
Sbjct: 68 EVIDWLGKLKDAVYDADDLLDDFSTHVLQRQVMMQGKRG-KQVSFLFSKANQVAYNLKIG 127
Query: 121 QKIKTVREKLDAIARDKAQLNLIENTREIRVDESTKLPETSSFILEGEVIGRDRDRKRIV 180
+IK +R++LDAIA DK + + + + + +T SF+ + V+GR+ D++ I+
Sbjct: 128 HQIKAIRQRLDAIAADKTKYHFTDRSLVSIPVVKVERKQTHSFVRKEGVVGREGDKEAIM 187
Query: 181 DFLLDTNTEMDNVKVIAIIGMGGLGKTALAQSIYGDEKVKEHFELTMWVCISEEFDVKVI 240
LLD++ DNV VI I+G+GG GKT +A+ +Y DEK+ +HFEL +WVC+S+ FDVK+I
Sbjct: 188 KRLLDSDVA-DNVSVIPIVGIGGQGKTTVAELVYNDEKIVKHFELRIWVCVSDVFDVKLI 247
Query: 241 VEKIIESLTKMRPEPNLQLNTLQSMLREKIDGKKYLLVMDDVWNVNHEKWISLKRYLMGG 300
+K++ES T + E NL++++LQ+ LR++IDG+KYLL++DDVWN N E+W +L+ LM G
Sbjct: 248 AQKMLESATNAKFE-NLEMDSLQTHLRKRIDGRKYLLILDDVWNDNRERWRNLRDLLMNG 307
Query: 301 AKGSRILITTRSHQVAETSETVLFHDLKELDKDNSWELFRKMAFSNESEELENSKLVVIG 360
A+GS+I++TTR+ VA + T + L+ L +D SW L KMAF E E NS+LV IG
Sbjct: 308 ARGSKIIVTTRAQVVASITGTTEPYLLEGLPEDMSWSLLEKMAFK-EGREPNNSRLVAIG 367
Query: 361 KEIVAKLKGSPLAIRIIGRLLYSKRSEKDYWLSFKENELRTVMQKENEIQSILKISFNHL 420
K+IV + G+PLAIR IG +LY+K +E + WLS KE +L + QK+ ++ ILK+S+ L
Sbjct: 368 KDIVKRCAGNPLAIRTIGGVLYTKDTETE-WLSLKEGQLLMITQKD-DVLPILKLSYEQL 427
Query: 421 SSSLKQCFTYCALFPKDYEIQKDELIKQWMAQGFIQP-HSKKAIEDVGDDYFKELLGRSF 480
S LKQCF YC+LFP+DYEI K LI WMA+GFIQ + +E++GD YF +LL RSF
Sbjct: 428 PSYLKQCFAYCSLFPRDYEINKQMLITLWMAEGFIQSLQGMQRLEELGDQYFMDLLRRSF 487
Query: 481 FQDIRKNKRGEIKEFKMHDFMHDLACSVGENDFVLVHDDTMPIDKGVRHVTFPFYLMKRW 540
FQD+ ++ G + KMHD MHDLA V +D +V D I + +RHV+F L W
Sbjct: 488 FQDVEYDEWGNVISCKMHDLMHDLAQLVAGSDSSMVDLDCKNISERIRHVSFNAELDSSW 547
Query: 541 KLIAKSLIEAKNLRTLNTP----HVHGIDLSNH---------LRLRTLNSEYFDSFPKCI 600
K I L++A +RT P H +D +H LRL L++ D P I
Sbjct: 548 K-IPTPLLKATKIRTFLLPVQPVHRVILDKVDHETVISSFRLLRLLDLHNTGIDILPSSI 607
Query: 601 GKMKHLRYVNLS-NISIHFLPKFFTKLYHLETLILRNCYLLRGLPGDIKNLINLRHLDIM 660
G +KHLRY++LS N I LP T+L +L+TL L +C L LP ++N+ +LRHL+
Sbjct: 608 GTLKHLRYLDLSKNEVIRRLPSSITELLNLQTLKLCSCKRLEELPRKLRNMTSLRHLETG 667
Query: 661 HCNSNNWTHMPIGLGSMTSLQTMNLFVLG----KNKGSKLSELNGLNSLRGSLKIRHLQF 720
C T MP GLG +TSLQT+ FV+G K L EL LN LRG + I L+
Sbjct: 668 QC--TGLTRMPSGLGQLTSLQTLTRFVVGMDSFKRPSGGLRELKDLNDLRGEVMIAKLEN 727
Query: 721 CKS--SDLENAKYLEEKFGIQKLELHWDRESKIDNALDDEDEKVLEGLKPHPNLHKIHIE 780
K+ S+ + A + K ++ L L W RE D+ +EDE++LEGL+PH NL + HI
Sbjct: 728 LKNVVSECKEAN-SKGKQHLEVLTLEWSREVN-DHTTFEEDEEILEGLQPHSNLQEFHIY 787
Query: 781 GFKGVKLCNWLSFDS---ISSLVTIKLRNCEKLQHLPRFDQFPFLKHLHLEELSNIEYID 840
G++ W+ D + +L+ I L C + HLP F P L+ L LE ++ +EYI+
Sbjct: 788 GYRAGSFPKWMLSDMSLVLPNLLEITLWRCNRCLHLPLFSHLPKLRVLRLEVVTAVEYIE 847
Query: 841 NNNFVSS-----------------STNFFPSLEKLSIISMPNLKGWWKGEMVTTNIIVG- 900
++ SS ST FFP L++L + + NLKGWW+ N +G
Sbjct: 848 DSGAESSSLSFGGNRLKGGTEGKESTAFFPCLKQLMLFDLRNLKGWWREVTAIANDNLGL 907
Query: 901 ---------EDSSSTNVFRSLSSLSISE-MDFEFLP-HDLFCNMTHLKSLHISHCKNLKM 960
+ S F LS L+I + ++P H L L+ L + C + K+
Sbjct: 908 AAASSQRPLQQKESMTSFPCLSKLTIGICTNLTYMPLHPL------LEELELK-CVSAKL 967
Query: 961 SSDHDDVIWKELESLHSLKLTFIPKLEYLPKGFQYMTAVEHLILADCPNLVCIEGIEHLT 1020
+I E E + + YL K + M + L P +G+ HLT
Sbjct: 968 -LQQSVMIAAETEQIPMAAAAHLSYPLYLSK-LKVMHIDSIMDLVSFPE----KGLHHLT 1027
Query: 1021 SLSSLTIFNCPSLTSLPQEMGQLTSLSSLTIIDCPNLTSLPQEMGQLTSLSSLGIFRCPN 1080
SL L+I NCP L LP+E L SL I C L SL + LT+L L I C
Sbjct: 1028 SLQHLSIANCPKLVCLPEE--GLNSLRFFFISGCDMLKSLSKGFRHLTALEELEIKECRE 1087
Query: 1081 LTSLPQEMDQ-------VTSLSSLTIDDITNLISLPQEMRQLTSLSSLTIIDCPNLTSLP 1101
L L +++++ + SL +L I D+ L SLP ++ +T+L L I C NL SLP
Sbjct: 1088 L-DLSKDVEENVMELRFLRSLRTLKIGDMPKLNSLPDGLQHVTTLKYLQISSCSNLKSLP 1140
BLAST of Lsi06G001610 vs. TAIR10
Match:
AT3G14470.1 (AT3G14470.1 NB-ARC domain-containing disease resistance protein)
HSP 1 Score: 412.5 bits (1059), Expect = 8.1e-115
Identity = 328/1067 (30.74%), Postives = 523/1067 (49.02%), Query Frame = 1
Query: 35 LHKLKATVSAIDAVLLDAEEQQFKSHAVKDWISKLKDAFYDIDDLMDEFSYESLRRQVMT 94
L +L + I AVL+DAEE+Q + V+ W+++L+D Y +D +D+ + E+LR +
Sbjct: 39 LERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLNIGA 98
Query: 95 KHRPNTKQVRIFFSKSNQIAFLLKMGQKIKTVREK----LDAIARDKAQLNLIENTREIR 154
+ ++ ++R + + FL + ++T EK L+ +A + L L E T I
Sbjct: 99 ESS-SSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGLKELTAMI- 158
Query: 155 VDESTKLPETSSFILEGEVIGRDRDRKRIVDFLLDTNTEMDNVKVIAIIGMGGLGKTALA 214
+LP T+S + E EV GRD D+ I+ FL+ N + + + V+AI+G+GG+GKT L+
Sbjct: 159 --PKQRLP-TTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGVGKTTLS 218
Query: 215 QSIYGDEKVKEHFELTMWVCISEEFDVKVIVEKIIESLTKMRPEPNLQLNTLQSMLREKI 274
Q +Y D+ V+ +F +W +SEEFDV I +K+ ES+T RP L+ LQ L+E++
Sbjct: 219 QLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTS-RPCEFTDLDVLQVKLKERL 278
Query: 275 DGK--KYLLVMDDVWNVNHEKWISLKRYLMGGAKGSRILITTRSHQVAETSETVLFHDLK 334
G +LLV+DD+WN N W L++ + A+GS+IL+TTRS +VA V H+L+
Sbjct: 279 TGTGLPFLLVLDDLWNENFADWDLLRQPFIHAAQGSQILVTTRSQRVASIMCAVHVHNLQ 338
Query: 335 ELDKDNSWELFRKMAFSNESEELENSKLVVIGKEIVAKLKGSPLAIRIIGRLLYSKRSEK 394
L + W LF K F N+ E N ++ + + IV K +G PLA++ +G +L +
Sbjct: 339 PLSDGDCWSLFMKTVFGNQ-EPCLNREIGDLAERIVHKCRGLPLAVKTLGGVLRFEGKVI 398
Query: 395 DYWLSFKENELRTVMQKENEIQSILKISFNHLSSSLKQCFTYCALFPKDYEIQKDELIKQ 454
+ W + + + ++ + +L++S+ +L + LK+CF YC++FPK + +KD+++
Sbjct: 399 E-WERVLSSRIWDLPADKSNLLPVLRVSYYYLPAHLKRCFAYCSIFPKGHAFEKDKVVLL 458
Query: 455 WMAQGFIQP-HSKKAIEDVGDDYFKELLGRSFFQDIRKNKRGEIKEFKMHDFMHDLACSV 514
WMA+GF+Q S K +E++G++YF EL RS Q + + MHDF+++LA
Sbjct: 459 WMAEGFLQQTRSSKNLEELGNEYFSELESRSLLQKTKTR-------YIMHDFINELA-QF 518
Query: 515 GENDFVLVHDDTMPIDKGVRHVTFPFYLMKRWK--LIAKSLIEAKNLRTL------NTPH 574
+F +D + R + YL + + ++L E K LRT N+
Sbjct: 519 ASGEFSSKFEDGCKLQVSER-TRYLSYLRDNYAEPMEFEALREVKFLRTFLPLSLTNSSR 578
Query: 575 VHGID-------LSNHLRLRTLNSEYFDSF---PKCIGKMKHLRYVNLSNISIHFLPKFF 634
+D L RLR L+ ++ P + H R+++LS + LPK
Sbjct: 579 SCCLDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFFKNISHARFLDLSRTELEKLPKSL 638
Query: 635 TKLYHLETLILRNCYLLRGLPGDIKNLINLRHLDIMHCNSNNWTHMPIGLGSMTSLQTMN 694
+Y+L+TL+L C L+ LP DI NLINLR+LD++ MP G + SLQT+
Sbjct: 639 CYMYNLQTLLLSYCSSLKELPTDISNLINLRYLDLI---GTKLRQMPRRFGRLKSLQTLT 698
Query: 695 LFVLGKNKGSKLSELNGLNSLRGSLKIRHLQ-FCKSSDLENAKYLEEKFGIQKLELHW-- 754
F + + GS++SEL GL+ L G LKI LQ +D A L K +++++ W
Sbjct: 699 TFFVSASDGSRISELGGLHDLHGKLKIVELQRVVDVADAAEAN-LNSKKHLREIDFVWRT 758
Query: 755 ---DRESKIDNALDDEDEKVLEGLKPHPNLHKIHIEGFKGVKLCNWLSFDSISSLVTIKL 814
E+ + + +V E L+PH ++ K+ IE +KG + +WLS S S +V I+L
Sbjct: 759 GSSSSENNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYKGRRFPDWLSDPSFSRIVCIRL 818
Query: 815 RNCEKLQHLPRFDQFPFLKHLHLEELSNIE------YIDNNNFVSSSTNFFPSLEKLSII 874
R C+ LP Q P LK LH+ + ++ Y + F SLE L
Sbjct: 819 RECQYCTSLPSLGQLPCLKELHISGMVGLQSIGRKFYFSDQQLRDQDQQPFRSLETLRFD 878
Query: 875 SMPNLKGWWKGEMVTTNIIVGEDSSSTNVFRSLSSLSISEMDFEFLPHDLFCNMTHLKSL 934
++P+ + W + + G+ +F SL L I + L L + L SL
Sbjct: 879 NLPDWQEW-----LDVRVTRGD------LFPSLKKLFI--LRCPELTGTLPTFLPSLISL 938
Query: 935 HISHCKNLKMSSDHDDVIWKELESLHSLKLTFIPKLEYLPKGFQYMTAVEHLILADCPNL 994
HI C L DH + ++ L++L I + C L
Sbjct: 939 HIYKCGLLDFQPDHHEYSYRNLQTLS--------------------------IKSSCDTL 998
Query: 995 VCIEGIEHLTSLSSLTIFNCPSLTSLPQEMGQL---TSLSSLTIIDCPNLTSLPQEMGQL 1054
V + H +L L + C SL SL L +L +L I DC NL LP+
Sbjct: 999 VKFP-LNHFANLDKLEVDQCTSLYSLELSNEHLRGPNALRNLRINDCQNLQLLPK----- 1016
Query: 1055 TSLSSLGIFRCPNLTSLPQEMDQVTSLSSLTIDDITNLISLPQEMRQ 1062
L +LPQ + QVT ITN L Q M Q
Sbjct: 1059 -------------LNALPQNL-QVT---------ITNCRYLRQPMEQ 1016
BLAST of Lsi06G001610 vs. TAIR10
Match:
AT3G14460.1 (AT3G14460.1 LRR and NB-ARC domains-containing disease resistance protein)
HSP 1 Score: 364.4 bits (934), Expect = 2.5e-100
Identity = 258/839 (30.75%), Postives = 430/839 (51.25%), Query Frame = 1
Query: 35 LHKLKATVSAIDAVLLDAEEQQFKSHAVKDWISKLKDAFYDIDDLMDEFSYESLRRQVMT 94
L +LK + + VL DA+++ VK W++ +KDAF+ +D++DE E+LRR+V+
Sbjct: 36 LKRLKVALVTANPVLADADQRAEHVREVKHWLTGIKDAFFQAEDILDELQTEALRRRVVA 95
Query: 95 KHRPNTKQVRIFFSKSNQIAFLLKMGQKIKTVREKLDAIARDKAQLNLIE--NTREIRVD 154
+ + + + A K+ K++ V L+ + + L E TRE +
Sbjct: 96 EAGGLGGLFQNLMA--GREAIQKKIEPKMEKVVRLLEHHVKHIEVIGLKEYSETREPQWR 155
Query: 155 ESTKLPETSSFILEGEVIGRDRDRKRIVDFLL-DTNTEMDNVKVIAIIGMGGLGKTALAQ 214
++++ + +G ++GR D+ +V+ LL D + VI+++GM G+GKT L +
Sbjct: 156 QASR--SRPDDLPQGRLVGRVEDKLALVNLLLSDDEISIGKPAVISVVGMPGVGKTTLTE 215
Query: 215 SIYGDEKVKEHFELTMWVCISEEFDVKVIVEKIIESLTKMRPEPNLQLNTLQSMLREKID 274
++ D +V EHFE+ MW+ F+V + + +++ +T L +LQ L++ +
Sbjct: 216 IVFNDYRVTEHFEVKMWISAGINFNVFTVTKAVLQDITSSAVNTE-DLPSLQIQLKKTLS 275
Query: 275 GKKYLLVMDDVWNVNHEKWISLKRYLMGGAKGSRILITTRSHQVAETSETVLFHDLKELD 334
GK++LLV+DD W+ + +W S + +GS+I++TTRS V+ ++ + +K +
Sbjct: 276 GKRFLLVLDDFWSESDSEWESFQVAFTDAEEGSKIVLTTRSEIVSTVAKAEKIYQMKLMT 335
Query: 335 KDNSWELFRKMAFSNESEELENSKLVVIGKEIVAKLKGSPLAIRIIGRLLYSKRSEKDYW 394
+ WEL + AF N S N +L IGK I + KG PLA R I L SK + D++
Sbjct: 336 NEECWELISRFAFGNISVGSINQELEGIGKRIAEQCKGLPLAARAIASHLRSKPNPDDWY 395
Query: 395 LSFKENELRTVMQKENEIQSILKISFNHLSSSLKQCFTYCALFPKDYEIQKDELIKQWMA 454
K T N I +LK+S++ L LK+CF C++FPK + ++EL+ WMA
Sbjct: 396 AVSKNFSSYT-----NSILPVLKLSYDSLPPQLKRCFALCSIFPKGHVFDREELVLLWMA 455
Query: 455 QGFI-QPHSKKAIEDVGDDYFKELLGRSFFQDIRKNKRGEIKEFKMHDFMHDLACSVGEN 514
+ QP S + +ED+G+DY +L+ +SFFQ + + F MHD M+DLA +V +
Sbjct: 456 IDLLYQPRSSRRLEDIGNDYLGDLVAQSFFQRLDIT----MTSFVMHDLMNDLAKAVSGD 515
Query: 515 DFVLVHDDTMP-IDKGVRHVTFPFYLMKRWKLIAKSLIEAKNLRTL---NTP-HVHGIDL 574
+ DD +P I RH +F + +S+ A+ LRT+ N+P + + L
Sbjct: 516 FCFRLEDDNIPEIPSTTRHFSFSRSQCDA-SVAFRSICGAEFLRTILPFNSPTSLESLQL 575
Query: 575 S-----------NHLRLRTLNSEYFDSFPKCIGKMKHLRYVNLSNISIHFLPKFFTKLYH 634
+ + LR+ +L+ + PK + +K LRY++LS+ I LP+F L +
Sbjct: 576 TEKVLNPLLNALSGLRILSLSHYQITNLPKSLKGLKLLRYLDLSSTKIKELPEFVCTLCN 635
Query: 635 LETLILRNCYLLRGLPGDIKNLINLRHLDIMHCNSNNWTHMPIGLGSMTSLQTMNLFVLG 694
L+TL+L NC L LP I LINLR LD++ MP G+ + SLQ ++ FV+G
Sbjct: 636 LQTLLLSNCRDLTSLPKSIAELINLRLLDLV---GTPLVEMPPGIKKLRSLQKLSNFVIG 695
Query: 695 KNKGSKLSELNGLNSLRGSLKIRHLQFCKSSDLENAKYLEEKFGIQKLELHWDRESK--- 754
+ G+ L EL L+ LRG+L+I LQ + L+ K + L L W +
Sbjct: 696 RLSGAGLHELKELSHLRGTLRISELQNVAFASEAKDAGLKRKPFLDGLILKWTVKGSGFV 755
Query: 755 --IDNALDDEDEKVLEGLKPHPNLHKIHIEGFKGVKLCNWLSFDSISSLVTIKLRNCEKL 814
NAL + ++VL L+PHP+L IE ++G WL S + ++ L +C
Sbjct: 756 PGSFNALACDQKEVLRMLEPHPHLKTFCIESYQGGAFPKWLGDSSFFGITSVTLSSCNLC 815
Query: 815 QHLPRFDQFPFLKHLHLEELSNIEYIDNNNFVSSSTNF---FPSLEKLSIISMPNLKGW 846
LP Q P LK+L +E+ + ++ + + F + + F SL+ L MP W
Sbjct: 816 ISLPPVGQLPSLKYLSIEKFNILQKVGLDFFFGENNSRGVPFQSLQILKFYGMPRWDEW 856
BLAST of Lsi06G001610 vs. TAIR10
Match:
AT3G46730.1 (AT3G46730.1 NB-ARC domain-containing disease resistance protein)
HSP 1 Score: 255.0 bits (650), Expect = 2.2e-67
Identity = 231/821 (28.14%), Postives = 386/821 (47.02%), Query Frame = 1
Query: 9 LAGNIITKLGSFAIQELGLLWGVHDELHKLKATVSAIDAVLLDAEEQQFKSHAVKDWISK 68
+ G ++ K+G + I E+ L GV D+L +LK ++ I L D E ++ + K+W
Sbjct: 5 VTGFVLNKIGGYLINEVLALMGVKDDLEELKTELTCIHGYLKDVEAREREDEVSKEWTKL 64
Query: 69 LKDAFYDIDDLMDEFSYESLRRQVMTKHRPNTKQVRIFFSKSNQIAFLLKMGQKIKTVRE 128
+ D YDI+D++D + + R + T ++ N + + + ++I +
Sbjct: 65 VLDIAYDIEDVLDTYFLKLEERSLRRGLLRLTNKIGKKRDAYNIVEDIRTLKRRILDITR 124
Query: 129 KLDAIARDKAQLNLIENTREIRVDESTKLPETSSFILEGEVIGRDRDRKRIVDFLLDTNT 188
K + EN +RV + + P E V+G + D K ++ LL N
Sbjct: 125 KRETFGIGSFNEPRGENITNVRVRQLRRAPPVDQ---EELVVGLEDDVKILLVKLLSDN- 184
Query: 189 EMDNVKVIAIIGMGGLGKTALAQSIYGDEKVKEHFELTMWVCISEEFDVKVIVEKIIESL 248
E D +I+I GMGGLGKTALA+ +Y VK F+ W +S+E+ + I+ +II SL
Sbjct: 185 EKDKSYIISIFGMGGLGKTALARKLYNSGDVKRRFDCRAWTYVSQEYKTRDILIRIIRSL 244
Query: 249 TKMRPEPNLQLNT------LQSMLREKIDGKKYLLVMDDVWNVNHEKWISLKRYLMGGAK 308
+ E ++ L+ L ++GK Y++V+DDVW+ + W SLKR L +
Sbjct: 245 GIVSAEEMEKIKMFEEDEELEVYLYGLLEGKNYMVVVDDVWDP--DAWESLKRALPCDHR 304
Query: 309 GSRILITTRSHQVAETSE-TVLFHDLKELDKDNSWELFRKMAFSNESEELENSKLVVIGK 368
GS+++ITTR +AE E TV H L+ L + SW LF + AFSN + E+ L GK
Sbjct: 305 GSKVIITTRIRAIAEGVEGTVYAHKLRFLTFEESWTLFERKAFSNIEKVDED--LQRTGK 364
Query: 369 EIVAKLKGSPLAIRIIGRLLYSKRSEKDYWLSFKENELRTVMQKENEIQSILKISFNHLS 428
E+V K G PLAI ++ LL KR+ + W + R + I ++ +SF +
Sbjct: 365 EMVKKCGGLPLAIVVLSGLLSRKRTNE--WHEVCASLWRRLKDNSIHISTVFDLSFKEMR 424
Query: 429 SSLKQCFTYCALFPKDYEIQKDELIKQWMAQGFIQPHSKKAIEDVGDDYFKELLGRSFFQ 488
LK CF Y ++FP+DYEI+ ++LI +A+GFIQ + +EDV Y EL+ RS +
Sbjct: 425 HELKLCFLYFSVFPEDYEIKVEKLIHLLVAEGFIQEDEEMMMEDVARCYIDELVDRSLVK 484
Query: 489 DIRKNKRGEIKEFKMHDFMHDLAC-SVGENDFVLV-----HDDTMPIDKGVRHVTFPFYL 548
+ +RG++ ++HD + DLA E +FV V H + + V H+ +YL
Sbjct: 485 -AERIERGKVMSCRIHDLLRDLAIKKAKELNFVNVYNEKQHSSDICRREVVHHLMNDYYL 544
Query: 549 MKRW--KLIAKSLI--EAKNLRTLNTPH--VHGIDLSNHLRLRTLNSEYFDSFPKCIGKM 608
R K + L E + +NT + + + + N L ++ ++ P IG++
Sbjct: 545 CDRRVNKRMRSFLFIGERRGFGYVNTTNLKLKLLRVLNMEGLLFVSKNISNTLPDVIGEL 604
Query: 609 KHLRYVNLSNISIHFLPKFFTKLYHLETLILRNCYLLRGLPGDIKNLINLRHLDIMHCNS 668
HLRY+ +++ + LP ++ NLR L + +
Sbjct: 605 IHLRYLGIADTYVSILP---------------------------ASISNLRFLQTLDASG 664
Query: 669 NNWTHMPIGLGSMTSLQTMNLFVLGK-------NKGSKLSELNGLNSLRGSLKIRHLQFC 728
N+ L +TSL+ V+GK +G L L ++S S K+ H
Sbjct: 665 NDPFQYTTDLSKLTSLR----HVIGKFVGECLIGEGVNLQTLRSISSYSWS-KLNHELLR 724
Query: 729 KSSDLE---NAKYLEEKFGIQKLELHWDRESKIDNALDDEDEKVLEGLKPHPNLHKIHIE 788
DLE ++K+++++ ++ L++ SK N L LK K+ E
Sbjct: 725 NLQDLEIYDHSKWVDQR----RVPLNFVSFSKPKN---------LRVLKLEMRNFKLSSE 768
Query: 789 GFKGVKLCNWLSFDSISSL----VTIKLRNCEKLQHLPRFD 797
+ L + ++F S+ SL T++ + LQ LPR +
Sbjct: 785 SRTTIGLVD-VNFPSLESLTLVGTTLEENSMPALQKLPRLE 768
BLAST of Lsi06G001610 vs. TAIR10
Match:
AT3G46530.1 (AT3G46530.1 NB-ARC domain-containing disease resistance protein)
HSP 1 Score: 246.1 bits (627), Expect = 1.0e-64
Identity = 209/740 (28.24%), Postives = 355/740 (47.97%), Query Frame = 1
Query: 13 IITKLGSFAIQELGLLWGVHDELHKLKATVSAIDAVLLDAEEQQFKSHAVKDWISKLKDA 72
++ K+G++ I+E + V ++L +LK ++ I L D E ++ + K+W + D
Sbjct: 9 VVGKIGNYLIEEASMFMAVKEDLEELKTELTCIHGYLKDVEAREREDEVSKEWSKLVLDF 68
Query: 73 FYDIDDLMDEFSYESLRRQVMTKHRPNTKQVRIFFSKSNQIAFLLKMGQKIKTVREKLDA 132
YD++D++D + + + R + +R +K + + I+ ++ ++
Sbjct: 69 AYDVEDVLDTY-------HLKLEERSQRRGLRRLTNKIGRKMDAYSIVDDIRILKRRILD 128
Query: 133 IARDKAQLNLIE--------NTREIRVDESTKLPETSSFILEGEVIGRDRDRKRIVDFLL 192
I R + + NT +RV + L S E V+G + D K +++ LL
Sbjct: 129 ITRKRETYGIGGLKEPQGGGNTSSLRVRQ---LRRARSVDQEEVVVGLEDDAKILLEKLL 188
Query: 193 DTNTEMDNVKVIAIIGMGGLGKTALAQSIYGDEKVKEHFELTMWVCISEEFDVKVIVEKI 252
D E N +I+I GMGGLGKTALA+ +Y VKE FE W +S+E+ I+ +I
Sbjct: 189 DY--EEKNRFIISIFGMGGLGKTALARKLYNSRDVKERFEYRAWTYVSQEYKTGDILMRI 248
Query: 253 IESLTKMRPEPNLQLNT-----LQSMLREKIDGKKYLLVMDDVWNVNHEKWISLKRYLMG 312
I SL E ++ L+ L ++GKKYL+V+DD+W E W SLKR L
Sbjct: 249 IRSLGMTSGEELEKIRKFAEEELEVYLYGLLEGKKYLVVVDDIWE--REAWDSLKRALPC 308
Query: 313 GAKGSRILITTRSHQVAETSETVLF-HDLKELDKDNSWELFRKMAFSNESEELENSKLVV 372
+GSR++ITTR VAE + + H L+ L + SWELF + AF N + E+ L+
Sbjct: 309 NHEGSRVIITTRIKAVAEGVDGRFYAHKLRFLTFEESWELFEQRAFRNIQRKDED--LLK 368
Query: 373 IGKEIVAKLKGSPLAIRIIGRLLYSKRSEK------DYWLSFKENELRTVMQKENEIQSI 432
GKE+V K +G PL I ++ LL K + W K++ + +
Sbjct: 369 TGKEMVQKCRGLPLCIVVLAGLLSRKTPSEWNDVCNSLWRRLKDDSIHVA-------PIV 428
Query: 433 LKISFNHLSSSLKQCFTYCALFPKDYEIQKDELIKQWMAQGFIQPHSKKAIEDVGDDYFK 492
+SF L K CF Y ++FP+DYEI ++LI +A+GFIQ + +EDV Y +
Sbjct: 429 FDLSFKELRHESKLCFLYLSIFPEDYEIDLEKLIHLLVAEGFIQGDEEMMMEDVARYYIE 488
Query: 493 ELLGRSFFQDIRKNKRGEIKEFKMHDFMHDLACSVGE--------NDFVLVHDDTMPIDK 552
EL+ RS + +R+ +RG++ ++HD + D+A + ND V H T +
Sbjct: 489 ELIDRSLLEAVRR-ERGKVMSCRIHDLLRDVAIKKSKELNFVNVYNDHVAQHSSTTCRRE 548
Query: 553 GVRHVTFPFYLMKRWKLIAKSLIEAKNLRTL----NTPHVHGIDLSNHLRLRTLNSEYFD 612
V H ++K + + K +R+ H+ G+D LR L+
Sbjct: 549 VVHH---------QFKRYSSEKRKNKRMRSFLYFGEFDHLVGLDFETLKLLRVLDFGSLW 608
Query: 613 SFPKCIGKMKHLRYVNLSNISIH--FLPKFFTKLYHLETLILRNCYLLRGLPGDIKNLIN 672
K G + HLRY+ + SI+ + +KL L+TL + + Y + D++ L +
Sbjct: 609 LPFKINGDLIHLRYLGIDGNSINDFDIAAIISKLRFLQTLFVSDNYFIEETI-DLRKLTS 668
Query: 673 LRHLDIMHCNSNNWTHMPIG-LGSMTSLQTMNLFVLGKNKGSKLSELN--GLNSLRGSLK 715
LRH+ N + + IG + ++ +L +++ K K L L G++ + S +
Sbjct: 669 LRHV-----IGNFFGGLLIGDVANLQTLTSISFDSWNKLKPELLINLRDLGISEMSRSKE 709
BLAST of Lsi06G001610 vs. TAIR10
Match:
AT3G07040.1 (AT3G07040.1 NB-ARC domain-containing disease resistance protein)
HSP 1 Score: 240.4 bits (612), Expect = 5.5e-63
Identity = 203/710 (28.59%), Postives = 330/710 (46.48%), Query Frame = 1
Query: 14 ITKLGSFAIQELGLLWGVHDELHKLKATVSAIDAVLLDAEEQ------QFKSHAVKDWIS 73
I ++ S E LL GVH E+ K+K + + + L D + + + +++
Sbjct: 10 IGRILSVLENETLLLSGVHGEIDKMKKELLIMKSFLEDTHKHGGNGSTTTTTQLFQTFVA 69
Query: 74 KLKDAFYDIDDLMDEFSYESLRRQVMTKHRPNTKQVRIFFSKSNQIAFLLKMGQKIKTVR 133
+D Y I+D++DEF Y + +R K R F A + QK+ V
Sbjct: 70 NTRDLAYQIEDILDEFGYH------IHGYRSCAKIWRAFHFPRYMWA-RHSIAQKLGMVN 129
Query: 134 EKLDAIARDKAQLNLIENTREIR---VDES-----TKLPETSSFILEGEVIGRDRDRKRI 193
+ +I+ + EN + +D+ + E+S F E ++G D + ++
Sbjct: 130 VMIQSISDSMKRYYHSENYQAALLPPIDDGDAKWVNNISESSLFFSENSLVGIDAPKGKL 189
Query: 194 VDFLLDTNTEMDNVKVIAIIGMGGLGKTALAQSIYGDEKVKEHFELTMWVCISEEFDVKV 253
+ LL + V+A++GMGG GKT L+ +I+ + V+ HFE WV IS+ + ++
Sbjct: 190 IGRLLSPEPQRI---VVAVVGMGGSGKTTLSANIFKSQSVRRHFESYAWVTISKSYVIED 249
Query: 254 IVEKIIESLTKMRPEP------NLQLNTLQSMLREKIDGKKYLLVMDDVWNVNHEKWISL 313
+ +I+ K +L L L E + K+Y++V+DDVW W +
Sbjct: 250 VFRTMIKEFYKEADTQIPAELYSLGYRELVEKLVEYLQSKRYIVVLDDVWTTG--LWREI 309
Query: 314 KRYLMGGAKGSRILITTRSHQVAETSETV--LFHDLKELDKDNSWELFRKMAFSNESEEL 373
L G GSR+++TTR VA + H+++ L +D +W LF AF E+
Sbjct: 310 SIALPDGIYGSRVMMTTRDMNVASFPYGIGSTKHEIELLKEDEAWVLFSNKAFPASLEQC 369
Query: 374 ENSKLVVIGKEIVAKLKGSPLAIRIIGRLLYSKRSEKDY--------WLSFKENELRTVM 433
L I +++V + +G PLAI +G ++ +K+ E ++ W +EL+ V
Sbjct: 370 RTQNLEPIARKLVERCQGLPLAIASLGSMMSTKKFESEWKKVYSTLNWELNNNHELKIV- 429
Query: 434 QKENEIQSILKISFNHLSSSLKQCFTYCALFPKDYEIQKDELIKQWMAQGFIQPHSKKAI 493
+SI+ +SFN L LK+CF YC+LFP +Y +++ LI+ WMAQ F++P
Sbjct: 430 ------RSIMFLSFNDLPYPLKRCFLYCSLFPVNYRMKRKRLIRMWMAQRFVEPIRGVKA 489
Query: 494 EDVGDDYFKELLGRSFFQDIRKNKRGEIKEFKMHDFMHDLACSVGEND-FVLVHDDTMPI 553
E+V D Y EL+ R+ Q I N G K FKMHD + ++A SV + + F V++D
Sbjct: 490 EEVADSYLNELVYRNMLQVILWNPFGRPKAFKMHDVIWEIALSVSKLERFCDVYNDDSDG 549
Query: 554 DK--------GVRHVTFPFYLMKRWKLIAKSLIEAKNLRTL--NTPHVHGIDL---SNHL 613
D G RH+ K + I A NL +L + H ++L N L
Sbjct: 550 DDAAETMENYGSRHLCIQ-------KEMTPDSIRATNLHSLLVCSSAKHKMELLPSLNLL 609
Query: 614 RLRTLNSEYFDSFPKCIGKMKHLRYVNLSNISIHFLPKFFTKLYHLETLILRNCYLLRGL 672
R L P C+ M +L+Y+NLS + LPK F KL +LETL ++ + L
Sbjct: 610 RALDLEDSSISKLPDCLVTMFNLKYLNLSKTQVKELPKNFHKLVNLETLNTKHS-KIEEL 669
BLAST of Lsi06G001610 vs. NCBI nr
Match:
gi|659099641|ref|XP_008450703.1| (PREDICTED: putative disease resistance protein RGA4 [Cucumis melo])
HSP 1 Score: 1362.1 bits (3524), Expect = 0.0e+00
Identity = 747/1126 (66.34%), Postives = 848/1126 (75.31%), Query Frame = 1
Query: 1 MADSILFNLAGNIITKLGSFAIQELGLLW-GVHDELHKLKATVSAIDAVLLDAEEQQFKS 60
MA+SILF LA NI TKLGSF + ELGLLW G H+EL KLK T+SAI AVLLDAEE+Q+KS
Sbjct: 1 MAESILFTLAANIATKLGSFPLHELGLLWTGFHEELDKLKDTLSAIQAVLLDAEEKQYKS 60
Query: 61 HAVKDWISKLKDAFYDIDDLMDEFSYESLRRQVMTKHRPNTKQVRIFFSKSNQIAFLLKM 120
+AVK+W+S+LKDAFYDIDDLMDEFSYES++RQVM KHR N KQVRIFFS+SNQIAF LKM
Sbjct: 61 YAVKEWVSRLKDAFYDIDDLMDEFSYESIKRQVMIKHRTNNKQVRIFFSESNQIAFRLKM 120
Query: 121 GQKIKTVREKLDAIARDKAQLNLIENTREIRVDESTKLPETSSFILEGEVIGRDRDRKRI 180
G KIK VREKLD +A DKAQ NL E TREIR DE+TK PETSSFILEGEVIGRD D+K I
Sbjct: 121 GYKIKRVREKLDIVAIDKAQFNLSEYTREIRNDETTKRPETSSFILEGEVIGRDDDKKGI 180
Query: 181 VDFLLDTNTEMDNVKVIAIIGMGGLGKTALAQSIYGDEKVKEHFELTMWVCISEEFDVKV 240
V FLLDTN +NV V+AIIGMGGLGKTALAQSIYGD K +HFELTMWVCISEEFDVK+
Sbjct: 181 VHFLLDTNVAQENVAVVAIIGMGGLGKTALAQSIYGDMKENKHFELTMWVCISEEFDVKI 240
Query: 241 IVEKIIESLTKMRPEPNLQLNTLQSMLREKIDGKKYLLVMDDVWNVNHEKWISLKRYLMG 300
IVEKIIESLTK RPEPNLQL+TLQ+MLREKIDGK+YLLVMDDVWNVN EKWI+LK +L G
Sbjct: 241 IVEKIIESLTKKRPEPNLQLDTLQNMLREKIDGKRYLLVMDDVWNVNREKWINLKAFLTG 300
Query: 301 GAKGSRILITTRSHQVAETSETVLFHDLKELDKDNSWELFRKMAFSNESEELENSKLVVI 360
GAKGSRILITTR+HQVA TSETVLFH L EL+K++SWELFRKMAFSNESE LENSKLV+I
Sbjct: 301 GAKGSRILITTRTHQVAHTSETVLFHHLSELNKNSSWELFRKMAFSNESEVLENSKLVII 360
Query: 361 GKEIVAKLKGSPLAIRIIGRLLYSKRSEKDYWLSFKENELRTVMQKENEIQSILKISFNH 420
GKEIVA LKGSPLA+R+IG LYSK++EKD WLSFK+NEL T+MQ+ENEIQSILKISFN
Sbjct: 361 GKEIVANLKGSPLALRVIGSYLYSKKTEKD-WLSFKDNELGTIMQQENEIQSILKISFNQ 420
Query: 421 LSSSLKQCFTYCALFPKDYEIQKDELIKQWMAQGFIQPHSKKAIEDVGDDYFKELLGRSF 480
LSS LKQCFTYCALFPKDYEIQKD+LIKQWMAQGFIQP +KK +EDVGDDYFKELLGRSF
Sbjct: 421 LSSGLKQCFTYCALFPKDYEIQKDDLIKQWMAQGFIQPQNKKTMEDVGDDYFKELLGRSF 480
Query: 481 FQDIRKNKRGEIKEFKMHDFMHDLACSVGENDFVLVHDDTMPIDKGVRHVTFPFYLMK-R 540
FQDI+KNK GEIKEFK+HDFMHDLACSV EN VL +DDT IDK RHV+ ++ K R
Sbjct: 481 FQDIKKNKWGEIKEFKIHDFMHDLACSVVENVCVLTNDDTKTIDKRTRHVSISTFISKTR 540
Query: 541 WKLIAKSLIEAKNLRTLNTPHVHGIDLSNHLRLRTLNSEYFDSFPKCIGKMKHLRYVNLS 600
WK+I +SL EAKNLRTLN IDLSNHL+LRTLN ++ PKCIGKMKHLRYVNLS
Sbjct: 541 WKVITESLKEAKNLRTLNYA-CDKIDLSNHLQLRTLNVDFLYHVPKCIGKMKHLRYVNLS 600
Query: 601 NISIHFLPKFFTKLYHLETLILRNCYLLRGLPGDIKNLINLRHLDIMHCNSNNWTHMPIG 660
N I FLPKF TKLY+LETLILRNC L GLP DIKNLINLRHLDIMHCNSN W+ MP+G
Sbjct: 601 NTWIDFLPKFITKLYNLETLILRNCQRLMGLPSDIKNLINLRHLDIMHCNSNCWSSMPMG 660
Query: 661 LGSMTSLQTMNLFVLGKNKGSKLSELNGLNSLRGSLKIRHLQFCKSSDLENAKYLEEKFG 720
LGSMTSLQTMNLFVLG G +LSELNGLNSLRGSL+I+ LQFC + +LENAKYLEEK G
Sbjct: 661 LGSMTSLQTMNLFVLGAKWGGELSELNGLNSLRGSLRIKQLQFCITPNLENAKYLEEKSG 720
Query: 721 IQKLELHWDRESKID-NALDDEDEKVLEGLKPHPNLHKIHIEGFKGVKLCNWLSFDSISS 780
IQKLELHWD I N +DEDEKVLE LKPHPNL KI IEG+KGVKLCNW SF SI S
Sbjct: 721 IQKLELHWDSSYGIGRNTFNDEDEKVLECLKPHPNLQKIRIEGYKGVKLCNWFSFGSIGS 780
Query: 781 LVTIKLRNCEKLQHLPRFDQFPFLKHLHLEELSNIEYIDNNNFVSSS-TNFFPSLEKLSI 840
LVTIKL CEKL+HLP+FDQFPFLKHLHLE+L NIE+ID+NN+VSSS T FFPSLEKLSI
Sbjct: 781 LVTIKLCECEKLKHLPQFDQFPFLKHLHLEDLPNIEFIDDNNYVSSSLTTFFPSLEKLSI 840
Query: 841 ISMPNLKGWWKGEMVTTNIIVGEDSSSTNVFRSLSSLSISEMDFEFLPHDLFCNMTHLKS 900
I +P LK WWKGE + T F + + HL
Sbjct: 841 IQLPKLKEWWKGEFI----------DQTTPFPT--------------------TLHHLSR 900
Query: 901 LHISHCKNLKMSSDHDDVIWKELESLHSLKLTFIPKLEYLPKGFQYMTAVEHLILADCPN 960
L I+HC L H L SL ++ + + E ++ N
Sbjct: 901 LKINHCPQLGSIPQHGP--------LRSLDVSGVS-----------LQLFELVMEMATTN 960
Query: 961 LVCIEGIEHLT--SLSSLTIFNCP-SLTSLPQEMGQLTSLSSLTIIDCPNLTSLPQEMG- 1020
++ + T SLSSL+I++ L +T L SL I +C N+ G
Sbjct: 961 IIVGQDSSSSTTRSLSSLSIWDMDFEFLQLHDLFSNMTHLKSLVIGNCKNIKMSSSRDGV 1020
Query: 1021 ---QLTSLSSLGIFRCPNLTSLPQEMDQVTSLSSLTIDDITNLISLPQEMRQLTSLSSLT 1080
+L SL L + P L LP+ + VT+L L + D NL+S+ + + LTSLS L
Sbjct: 1021 IWKELGSLRRLDLCSIPELECLPKGLQYVTTLECLKLYDCRNLVSI-EGIEHLTSLSLLE 1073
Query: 1081 IIDCPNLTSLPQEMGQLTSLSSLIIIDCPNLTFASRDGSTHLTIKF 1116
I CPNL S PQEMGQLTSLS L I CPNL + +G H+T +
Sbjct: 1081 IEYCPNLISYPQEMGQLTSLSRLRINGCPNLP-SLPEGLRHVTFMY 1073
BLAST of Lsi06G001610 vs. NCBI nr
Match:
gi|778662590|ref|XP_011659913.1| (PREDICTED: putative disease resistance protein RGA4 [Cucumis sativus])
HSP 1 Score: 1305.8 bits (3378), Expect = 0.0e+00
Identity = 709/1040 (68.17%), Postives = 806/1040 (77.50%), Query Frame = 1
Query: 1 MADSILFNLAGNIITKLGSFAIQELGLLW-GVHDELHKLKATVSAIDAVLLDAEEQQFKS 60
MA+SILF+LA NI TKLGS A+Q+LGLLW G+H+E+ KL+ T+SAI AVL DAE++Q+KS
Sbjct: 1 MAESILFSLAANIATKLGSLALQDLGLLWTGIHEEIDKLRDTLSAIQAVLHDAEQKQYKS 60
Query: 61 HAVKDWISKLKDAFYDIDDLMDEFSYESLRRQVMTKHRPN--TKQVRIFFSKSNQIAFLL 120
AVK+W+S+LKDAFYD+DDLMDEFSYES +RQVMTKHR N TKQV IFFSKSNQI F L
Sbjct: 61 SAVKEWVSRLKDAFYDMDDLMDEFSYESFQRQVMTKHRTNNCTKQVCIFFSKSNQIRFRL 120
Query: 121 KMGQKIKTVREKLDAIARDKAQLNLIENTREIRVDESTKLPETSSFILEGEVIGRDRDRK 180
KM KIK +REKLD I +DK Q NL +NTREIR DE T+ ET SFILEGEVIGRD D+K
Sbjct: 121 KMVHKIKKIREKLDTIDKDKTQFNLFDNTREIRNDEMTQRSETCSFILEGEVIGRDDDKK 180
Query: 181 RIVDFLLDTNT-EMDNVKVIAIIGMGGLGKTALAQSIYGDEKVKEHFELTMWVCISEEFD 240
IV FLLDTN +N+ V+AIIGMGGLGKTALAQSIYG K +HFELTMWVCISEEFD
Sbjct: 181 CIVHFLLDTNIIAKENIVVVAIIGMGGLGKTALAQSIYGHMKENKHFELTMWVCISEEFD 240
Query: 241 VKVIVEKIIESLTKMRPEPNLQLNTLQSMLREKIDGKKYLLVMDDVWNVNHEKWISLKRY 300
VKVIVEKIIESLTK RPEPNL L+TLQSMLREKIDGKKYLLVMDDVWN KWI+LK++
Sbjct: 241 VKVIVEKIIESLTKKRPEPNLTLDTLQSMLREKIDGKKYLLVMDDVWNDERTKWINLKKF 300
Query: 301 LMGGAKGSRILITTRSHQVAETSETVLFHDLKELDKDNSWELFRKMAFSNESEELENSKL 360
LMGGAKGSRILITTR+HQVA +T LFHDL ELDKDNSWELFRKMAFSNESE LENSKL
Sbjct: 301 LMGGAKGSRILITTRTHQVAHIFDTDLFHDLSELDKDNSWELFRKMAFSNESEMLENSKL 360
Query: 361 VVIGKEIVAKLKGSPLAIRIIGRLLYSKRSEKDYWLSFKENELRTVMQKENEIQSILKIS 420
V IGKEIVAKLKGSPLAIR+IG LYSK+SEKD WLSFKENEL T+MQ++NEIQSILKIS
Sbjct: 361 VGIGKEIVAKLKGSPLAIRVIGSYLYSKKSEKD-WLSFKENELDTIMQQKNEIQSILKIS 420
Query: 421 FNHLSSSLKQCFTYCALFPKDYEIQKDELIKQWMAQGFIQPHSKKAIEDVGDDYFKELLG 480
FNHLSSSLKQCFTYCALF KD+EI KD+LIKQWM +GFIQPH+KKA+EDVGD+YFKELLG
Sbjct: 421 FNHLSSSLKQCFTYCALFLKDFEIDKDDLIKQWMGEGFIQPHNKKAMEDVGDEYFKELLG 480
Query: 481 RSFFQDIRKNKRGEIKEFKMHDFMHDLACSVGENDFVLVHDDTMPIDKGVRHVTF-PFYL 540
RSFFQDI KN+ GEI +FKMHDFMHDLAC VGEND+V DDT IDK RH++ PF
Sbjct: 481 RSFFQDISKNQLGEIMKFKMHDFMHDLACFVGENDYVFATDDTKFIDKRTRHLSISPFIS 540
Query: 541 MKRWKLIAKSLIEAKNLRTLNTP-HVHG-----IDLSNHLRLRTLNSEYFDSFPKCIGKM 600
RW++I +SLI AKNLRTLN H + ID SNHLRLRTLN + PKCIGKM
Sbjct: 541 KTRWEVIKESLIAAKNLRTLNYACHNYDGDEIEIDFSNHLRLRTLNLIFSTHVPKCIGKM 600
Query: 601 KHLRYVNLSNISIHFLPKFFTKLYHLETLILRNCYLLRGLPGDIKNLINLRHLDIMHCNS 660
KHLRY+N + FLPK TKLYHLETLI R C+ LR LP DI NLINLRHL I +
Sbjct: 601 KHLRYINFTRCYFDFLPKVVTKLYHLETLIFRECFKLRELPSDITNLINLRHLGI-NSLI 660
Query: 661 NNWTHMPIGLGSMTSLQTMNLFVLGKNKGSKLSELNGLNSLRGSLKIRHLQFCKSSDLEN 720
++MP G+GSMT+LQT+NLF+LG+N+G +LSELNGL +LRGSL I+ LQFCK +E+
Sbjct: 661 EGLSYMPKGMGSMTTLQTLNLFILGENEGGELSELNGLINLRGSLSIQQLQFCKPIGIES 720
Query: 721 AKYLEEKFGIQKLELHWDR-ESKIDNALDDEDEKVLEGLKPHPNLHKIHIEGFKGVKLCN 780
AK+LEEK GIQKL+L+W E K + +DDEDEKVLE LKPHPNL KI I G+ GVKLCN
Sbjct: 721 AKHLEEKSGIQKLKLYWYHLERKYE--IDDEDEKVLECLKPHPNLQKIVINGYGGVKLCN 780
Query: 781 WLSFDSISSLVTIKLRNCEKLQHLPRFDQFPFLKHLHLEELSNIEYIDNNNFVSSS-TNF 840
W SFD I +LV I L NC KLQ LPRFDQFPFLKHL L+ L N+E+IDNN+ VSSS T F
Sbjct: 781 WFSFDYIVNLVIIDLFNCNKLQQLPRFDQFPFLKHLKLQYLPNVEFIDNNDSVSSSLTTF 840
Query: 841 FPSLEKLSIISMPNLKGWWKG------------------------------EMVTTNIIV 900
FPSLEKL I +P LK WWK EM TTNIIV
Sbjct: 841 FPSLEKLRIFRLPKLKEWWKRKLIDQTIPQHRRLESLNISGVSLQVFELVMEMATTNIIV 900
Query: 901 G-EDSSSTNVFRSLSSLSISEMDFEFLP-HDLFCNMTHLKSLHISHCKNLKMSSDHDDVI 960
G +DSSS+ SLS LSI ++DFEFL HDLF NMTHLKSL I +CKN+KMSS D V
Sbjct: 901 GSQDSSSSTTSISLSFLSIEDIDFEFLQFHDLFSNMTHLKSLWIINCKNIKMSSSLDAVT 960
Query: 961 WKELESLHSLKLTFIPKLEYLPKGFQYMTAVEHLILADCPNLVCIEGIEHL-TSLSSLTI 995
WK L SL L L+ IP LEYLPK Q +T ++ L + +CPNLV IE I HL TSLS L I
Sbjct: 961 WKGLGSLRELMLSSIPDLEYLPKSLQCVTTLQSLQIYNCPNLVSIESIRHLTTSLSVLEI 1020
BLAST of Lsi06G001610 vs. NCBI nr
Match:
gi|778728520|ref|XP_011659433.1| (PREDICTED: putative disease resistance protein RGA4 [Cucumis sativus])
HSP 1 Score: 1216.1 bits (3145), Expect = 0.0e+00
Identity = 667/1132 (58.92%), Postives = 821/1132 (72.53%), Query Frame = 1
Query: 1 MADSILFNLAGNIITKLGSFAIQELGLLWGVHDELHKLKATVSAIDAVLLDAEEQQFKSH 60
MADSILFN+A N+ITKLGS A++ELG LWGV+DEL KL+ +SAI AVLLDAEEQQ SH
Sbjct: 27 MADSILFNVAANVITKLGSSALRELGSLWGVNDELGKLQNILSAIKAVLLDAEEQQSVSH 86
Query: 61 AVKDWISKLKDAFYDIDDLMDEFSYESLRRQVMTKHRPNTKQVRIFFSKSNQIAFLLKMG 120
AVKDWISKL+D FYD+DDL+DEFSYE+LRRQV+TK R TKQV IFFSKSNQ++F KM
Sbjct: 87 AVKDWISKLRDVFYDVDDLIDEFSYETLRRQVLTKDRTITKQVCIFFSKSNQVSFGHKMS 146
Query: 121 QKIKTVREKLDAIARDKAQLNLIENTREIRVDESTKLPETSSFILEGEVIGRDRDRKRIV 180
QKIK VREKLDAIA DK QL+L RE R DE K+ ET SFI +GEVIGRD D+K I+
Sbjct: 147 QKIKQVREKLDAIANDKTQLHLSVRMRETRDDELRKMRETCSFIPKGEVIGRDDDKKAII 206
Query: 181 DFLLDTNTEMDNVKVIAIIGMGGLGKTALAQSIYGDEKVKEHFELTMWVCISEEFDVKVI 240
DFLLDTNT DNV+V++I+GMGGLGKTA+AQS+Y DEK+ EHF+L +WVCIS+EFD+KVI
Sbjct: 207 DFLLDTNTMEDNVEVVSIVGMGGLGKTAVAQSVYNDEKINEHFKLKLWVCISQEFDIKVI 266
Query: 241 VEKIIESLTKMRPEPNLQLNTLQSMLREKIDGKKYLLVMDDVWNVNHEKWISLKRYLMGG 300
VEKIIE + K +P+ +LQL+ LQSML+EKIDGKKYLLVMDDVWN +HE W+SLKR+LMGG
Sbjct: 267 VEKIIEFIAKKKPD-SLQLDILQSMLQEKIDGKKYLLVMDDVWNESHETWVSLKRFLMGG 326
Query: 301 AKGSRILITTRSHQVAETSETVLFHDLKELDKDNSWELFRKMAFSNESEELENSKLVVIG 360
AKGSRILITTR+ QVA+ S+TV FH LKELD ++SW LFRKMAF NE EE+ENS V IG
Sbjct: 327 AKGSRILITTRNLQVAQASDTVQFHHLKELDNESSWALFRKMAFLNEEEEIENSNKVRIG 386
Query: 361 KEIVAKLKGSPLAIRIIGRLLYSKRSEKDYWLSFKENELRTVMQKENEIQSILKISFNHL 420
KEI+AKLKGSPL IRI+GRLLY K +E D WLSFK+N+L T++Q+EN+IQ ILKISFNHL
Sbjct: 387 KEIIAKLKGSPLTIRIVGRLLYFKNTEMD-WLSFKDNDLGTILQQENQIQPILKISFNHL 446
Query: 421 SSSLKQCFTYCALFPKDYEIQKDELIKQWMAQGFIQPHSKKAIEDVGDDYFKELLGRSFF 480
S+LK CFTYCALFPKDYE QKD L+KQWMAQGFIQ HS K IEDVGDDYFKELLGRSFF
Sbjct: 447 PSNLKHCFTYCALFPKDYEFQKDGLVKQWMAQGFIQSHSNKEIEDVGDDYFKELLGRSFF 506
Query: 481 QDIRKNKRGEIKEFKMHDFMHDLACSVGENDFVLVHDDTMPIDKGVRHVTFPF-YLMKRW 540
+++ NK G++KE KMHD +HDLAC + EN+ V D T IDK RHV+FP Y K W
Sbjct: 507 HNVKVNKWGDVKECKMHDLIHDLACWIVENECVDASDKTKSIDKRTRHVSFPSNYSRKSW 566
Query: 541 KLIAKSLIEAKNLRTLNTPHVHGIDLSNHLRLRTLNSEY--FDSFPKCIGKMKHLRYVNL 600
+L AKSL E KNLRTL+ P + NHLRLR+LN Y F PK I +++HLRY+++
Sbjct: 567 ELEAKSLTEVKNLRTLHGPPF--LLSENHLRLRSLNLGYSKFQKIPKFISQLRHLRYLDI 626
Query: 601 SNISIHFLPKFFTKLYHLETLILRNCYLLRGLPGDIKNLINLRHLDIMHCNSNNWTHMPI 660
S+ + FLPKF TKLY+LETLILR+C LR LP DI NLINL+HLD+ C THMP
Sbjct: 627 SDHDMKFLPKFITKLYNLETLILRHCSDLRELPTDINNLINLKHLDVHGCY--RLTHMPK 686
Query: 661 GLGSMTSLQTMNLFVLGKNKGSKLSELNGLNSLRGSLKIRHLQFCKSSDLENAKYLEEKF 720
GLG +TSLQTMNLFVLGK+KG LSELN L LRGSL I+ L+ C ++DL+NAKY+EEKF
Sbjct: 687 GLGGLTSLQTMNLFVLGKDKGCDLSELNELARLRGSLLIKGLELCTTTDLKNAKYMEEKF 746
Query: 721 GIQKLELHWDRE---SKIDNALDDEDEKVLEGLKPHPNLHKIHIEGFKGVKLCNWLSFDS 780
GIQKL+L W+R+ ++ D A +++DE+VL+ LKPH N+HK+ I G++GVKLCNWLSFD
Sbjct: 747 GIQKLKLRWNRDLYDAETDYASENDDERVLDCLKPHSNVHKMQIRGYRGVKLCNWLSFDY 806
Query: 781 ISSLVTIKLRNCEKLQHLPRFDQFPFLKHLHLEELSNIEYIDNNNFVSSSTNFFPSLEKL 840
+ LV I+L++CEKLQHLP+FDQFPFLKHL LE L +IEYIDNNN +SSST FFPSLEKL
Sbjct: 807 LGGLVNIELQSCEKLQHLPQFDQFPFLKHLLLENLPSIEYIDNNNSLSSST-FFPSLEKL 866
Query: 841 SIISMPNLKGWWKGEMVTTNIIVGEDSSSTNVFRSLSSLSISEMDFEFLPHDLFCNMTHL 900
+I++MPNLKGWWKGE E + + +F ++ + HL
Sbjct: 867 TIMTMPNLKGWWKGETPP------ESARYSALFPTI--------------------LHHL 926
Query: 901 KSLHISHCKNLKMSSDHDDVIWKELESLHSLKLTFIPKLEYLPKGFQYMTAVEHLILADC 960
L IS+C L H + L + + K+ P +A+ L +
Sbjct: 927 SRLDISNCPQLASIPQHPPLRSLALNDVSVQLFDMVIKMATTPAA-DSSSALSKLSILHI 986
Query: 961 PNL----VCIEGIEHLTSLSSLTIFNCPSLTSLPQEMGQLTSLS----------SLTIID 1020
N+ + E T L T+ NC +L + + SL I D
Sbjct: 987 QNIDLEFLPEELFGSTTDLEIFTVVNCKNLQMSSSHLVDEDNDGVLGKKLGNLHSLGIFD 1046
Query: 1021 CPNLTSLPQEMGQLTSLSSLGIFRCPNLTSLPQEMDQVTSLSSLTIDDITNLISLPQEMR 1080
P L L +E+ +T+L L ++ CPN+ SL + + +TSLSSL I + +NL SLP+ +
Sbjct: 1047 MPQLEYLWKELKYMTTLERLDLYNCPNIVSL-EGISHLTSLSSLRICNCSNLTSLPEGIS 1106
Query: 1081 QLTSLSSLTIIDCPNLTSLPQEMGQLTSLSSLIIIDCPNLTFASRDGSTHLT 1113
LTSLS LTI+ CPNLTSLP +G LTSLS+L+I C NLT + +G +HLT
Sbjct: 1107 HLTSLSYLTIVCCPNLTSLPAGIGHLTSLSTLLIKYCVNLT-SLPEGVSHLT 1122
BLAST of Lsi06G001610 vs. NCBI nr
Match:
gi|778728533|ref|XP_011659435.1| (PREDICTED: putative disease resistance protein RGA1 isoform X2 [Cucumis sativus])
HSP 1 Score: 1157.5 bits (2993), Expect = 0.0e+00
Identity = 654/1140 (57.37%), Postives = 810/1140 (71.05%), Query Frame = 1
Query: 1 MADSILFNLAGNIITKLGSFAIQELGLLWGVHDELHKLKATVSAIDAVLLDAEEQQFKSH 60
MADS+LFN+A ++ITKLGS A++ELG LWGV+DEL KL+ T+SAI AVLLDAEEQQ KSH
Sbjct: 1 MADSVLFNVAASVITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSH 60
Query: 61 AVKDWISKLKDAFYDIDDLMDEFSYESLRRQVMTKHRPNTKQVRIFFSKSNQIAFLLKMG 120
VKDWI+K+KD FYDIDDL+DEFSYE+LRRQV+TK R TKQVRIFFSKSNQIAF KMG
Sbjct: 61 TVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMG 120
Query: 121 QKIKTVREKLDAIARDKAQLNLIENTREIRVDESTKLPETSSFILEGEVIGRDRDRKRIV 180
Q IK VREKLDAIA KAQL+L RE+R +E K+ ETSSFI EGE+IGRD DRK ++
Sbjct: 121 QTIKKVREKLDAIAAIKAQLHLSVCAREVRDNEPRKVRETSSFIPEGEIIGRDEDRKSVM 180
Query: 181 DFLLDT-NTEMDNVKVIAIIGMGGLGKTALAQSIYGDEKVKEHFELTMWVCISEEFDVKV 240
DFLL+T N DNV+V++I+GMGGLGKTALAQ++Y DEK+ F+ +WVCIS+EFD+KV
Sbjct: 181 DFLLNTSNITKDNVEVVSIVGMGGLGKTALAQTVYNDEKINNRFKWKIWVCISQEFDIKV 240
Query: 241 IVEKIIESLTKMRPEPNLQLNTLQSMLREKIDGKKYLLVMDDVWNVNHEKWISLKRYLMG 300
IVEKI+ES+TK + E +LQL+ LQSML+EKI GKKYLLVMDDVWNV+HEKWI LKR+LMG
Sbjct: 241 IVEKILESITKTKQE-SLQLDILQSMLQEKIYGKKYLLVMDDVWNVDHEKWIGLKRFLMG 300
Query: 301 GAKGSRILITTRSHQVAETSETVLFHDLKELDKDNSWELFRKMAFSNESEELENSKLVVI 360
GA GS+IL+TTR+ Q A+ S+TV FH LKELDKDNSW LFRKMAF N+ EELENS LV I
Sbjct: 301 GASGSKILVTTRNLQTAQASDTVWFHHLKELDKDNSWALFRKMAFLNKEEELENSNLVRI 360
Query: 361 GKEIVAKLKGSPLAIRIIGRLLYSKRSEKDYWLSFKENELRTVMQKENEIQSILKISFNH 420
GKEIVAKLKG PL+IR++GRLLY K +E D W SFK+NEL +++Q++++IQ ILKISFNH
Sbjct: 361 GKEIVAKLKGYPLSIRVVGRLLYFKNTEMD-WSSFKDNELDSILQEDDQIQPILKISFNH 420
Query: 421 LSSSLKQCFTYCALFPKDYEIQKDELIKQWMAQGFIQPHSKKAIEDVGDDYFKELLGRSF 480
L LKQCFTYCALFPKDYE +K+ L+KQWMAQGFIQ H+KKAIEDVGDDYF+EL+GRSF
Sbjct: 421 LPPKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFIQAHNKKAIEDVGDDYFQELVGRSF 480
Query: 481 FQDIRKNKRGEIKEFKMHDFMHDLACSVGENDFVLVHDDTMPIDKGVRHVTFPFYLMKRW 540
FQDIRKNK G++K KMHD +HDLACS+GEN+ V+V DD IDK RH +F
Sbjct: 481 FQDIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHASFLLSKRLTR 540
Query: 541 KLIAKSLIEAKNLRTLN------------TPHVHGIDLSNHLRLRTLNSEYFDSF-PKCI 600
++++KS IE +LRTL+ T H+ N +LRTLN + PK +
Sbjct: 541 EVVSKSSIEVTSLRTLDIDSRASFRSFKKTCHM------NLFQLRTLNLDRCCCHPPKFV 600
Query: 601 GKMKHLRYVNLSNISIHFLPKFFTKLYHLETLILRNCYLLRGLPGDIKNLINLRHLDIMH 660
K+KHLRY+NLS +++ FLP T LY+LETLILR C LR LP DI NLINLRHLDI
Sbjct: 601 DKLKHLRYLNLSGLNVTFLPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLDIYD 660
Query: 661 CNSNNWTHMPIGLGSMTSLQTMNLFVLGKNKGSKLSELNGLNSLRGSLKIRHLQFCKSSD 720
C+S THMP GLG MTSLQTM++FVLGKNKG LS LNGL SLRG L I+ LQFC ++D
Sbjct: 661 CSS--LTHMPKGLGGMTSLQTMSMFVLGKNKGGDLSALNGLKSLRGLLCIKGLQFCTTAD 720
Query: 721 LENAKYLEEKFGIQKLELHWDRESKIDNALDD---EDEKVLEGLKPHPNLHKIHIEGFKG 780
L+N YL+E +GIQKLELHWD + ++ALDD +DE VLEGLKPH N+ K+ I+G++G
Sbjct: 721 LKNVSYLKEMYGIQKLELHWDIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYRG 780
Query: 781 VKLCNWLSFDSISSLVTIKLRNCEKLQHLPRFDQFPFLKHLHLEELSNIEYIDNNNFVSS 840
+KLC+W S + + LV+I+L +CEKL+HLP+FDQF +LKHL L L NIEYID+ N VSS
Sbjct: 781 MKLCDWFSSNFLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYLPNIEYIDSGNSVSS 840
Query: 841 STNFFPSLEKLSIISMPNLKGWWKGEMVTTNIIVGEDSSSTNVFRSLSSLSI-------S 900
ST FFPSLEKL I SMP LKGWWKGE+ S T + LS L I S
Sbjct: 841 STTFFPSLEKLRIESMPKLKGWWKGEI----------SFPTTILHQLSELCIFYCPLLAS 900
Query: 901 EMDFEFLPHDLFCNMT-HLKSLHISHCKNLKMSSDHDDVIWKELESLHSLKLTFIPKLEY 960
L C ++ L + I +L S L L L++ I LE+
Sbjct: 901 IPQHPSLESLRICGVSVQLFQMVIRMATDLSEHSSSSST----LSKLSFLEIGTI-DLEF 960
Query: 961 LP-KGFQYMTAVEHLILADCPNLVCIEG----------IEHLTSLSSLTIFNCPSLTSLP 1020
LP + F MT +E LI+ C +L + L++L +L + + L P
Sbjct: 961 LPVELFCNMTHLESLIIERCKSLQMSSPHPVDEDNDVVWKKLSNLRTLRLESILKLEYFP 1020
Query: 1021 QEMGQLTSLSSLTIIDCPNLTSLPQEMGQLTSLSSLGIFRCPNLTSLPQEMDQVTSLSSL 1080
+ + +TSL +L + +C NL S + +G+L SLS L I RCPNL L +++ + SLS L
Sbjct: 1021 KSLKYITSLETLKLSNCENLVS-TEGIGELISLSHLEIDRCPNLPILSEDVGDLISLSHL 1080
Query: 1081 TIDDITNLISLPQEMRQLTSLSSLTIIDCPNLTSLPQEM----GQLTSLSSLIIIDCPNL 1101
I + L SL + + +LTSLSSL + DCPNL SLPQE L L I++CP L
Sbjct: 1081 LIWNCPKLTSLSEGITRLTSLSSLCLEDCPNLVSLPQEFLHHHSSLPGGRFLRILNCPKL 1114
BLAST of Lsi06G001610 vs. NCBI nr
Match:
gi|778728529|ref|XP_011659434.1| (PREDICTED: putative disease resistance protein RGA1 isoform X1 [Cucumis sativus])
HSP 1 Score: 1157.5 bits (2993), Expect = 0.0e+00
Identity = 654/1140 (57.37%), Postives = 810/1140 (71.05%), Query Frame = 1
Query: 1 MADSILFNLAGNIITKLGSFAIQELGLLWGVHDELHKLKATVSAIDAVLLDAEEQQFKSH 60
MADS+LFN+A ++ITKLGS A++ELG LWGV+DEL KL+ T+SAI AVLLDAEEQQ KSH
Sbjct: 1 MADSVLFNVAASVITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSH 60
Query: 61 AVKDWISKLKDAFYDIDDLMDEFSYESLRRQVMTKHRPNTKQVRIFFSKSNQIAFLLKMG 120
VKDWI+K+KD FYDIDDL+DEFSYE+LRRQV+TK R TKQVRIFFSKSNQIAF KMG
Sbjct: 61 TVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMG 120
Query: 121 QKIKTVREKLDAIARDKAQLNLIENTREIRVDESTKLPETSSFILEGEVIGRDRDRKRIV 180
Q IK VREKLDAIA KAQL+L RE+R +E K+ ETSSFI EGE+IGRD DRK ++
Sbjct: 121 QTIKKVREKLDAIAAIKAQLHLSVCAREVRDNEPRKVRETSSFIPEGEIIGRDEDRKSVM 180
Query: 181 DFLLDT-NTEMDNVKVIAIIGMGGLGKTALAQSIYGDEKVKEHFELTMWVCISEEFDVKV 240
DFLL+T N DNV+V++I+GMGGLGKTALAQ++Y DEK+ F+ +WVCIS+EFD+KV
Sbjct: 181 DFLLNTSNITKDNVEVVSIVGMGGLGKTALAQTVYNDEKINNRFKWKIWVCISQEFDIKV 240
Query: 241 IVEKIIESLTKMRPEPNLQLNTLQSMLREKIDGKKYLLVMDDVWNVNHEKWISLKRYLMG 300
IVEKI+ES+TK + E +LQL+ LQSML+EKI GKKYLLVMDDVWNV+HEKWI LKR+LMG
Sbjct: 241 IVEKILESITKTKQE-SLQLDILQSMLQEKIYGKKYLLVMDDVWNVDHEKWIGLKRFLMG 300
Query: 301 GAKGSRILITTRSHQVAETSETVLFHDLKELDKDNSWELFRKMAFSNESEELENSKLVVI 360
GA GS+IL+TTR+ Q A+ S+TV FH LKELDKDNSW LFRKMAF N+ EELENS LV I
Sbjct: 301 GASGSKILVTTRNLQTAQASDTVWFHHLKELDKDNSWALFRKMAFLNKEEELENSNLVRI 360
Query: 361 GKEIVAKLKGSPLAIRIIGRLLYSKRSEKDYWLSFKENELRTVMQKENEIQSILKISFNH 420
GKEIVAKLKG PL+IR++GRLLY K +E D W SFK+NEL +++Q++++IQ ILKISFNH
Sbjct: 361 GKEIVAKLKGYPLSIRVVGRLLYFKNTEMD-WSSFKDNELDSILQEDDQIQPILKISFNH 420
Query: 421 LSSSLKQCFTYCALFPKDYEIQKDELIKQWMAQGFIQPHSKKAIEDVGDDYFKELLGRSF 480
L LKQCFTYCALFPKDYE +K+ L+KQWMAQGFIQ H+KKAIEDVGDDYF+EL+GRSF
Sbjct: 421 LPPKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFIQAHNKKAIEDVGDDYFQELVGRSF 480
Query: 481 FQDIRKNKRGEIKEFKMHDFMHDLACSVGENDFVLVHDDTMPIDKGVRHVTFPFYLMKRW 540
FQDIRKNK G++K KMHD +HDLACS+GEN+ V+V DD IDK RH +F
Sbjct: 481 FQDIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHASFLLSKRLTR 540
Query: 541 KLIAKSLIEAKNLRTLN------------TPHVHGIDLSNHLRLRTLNSEYFDSF-PKCI 600
++++KS IE +LRTL+ T H+ N +LRTLN + PK +
Sbjct: 541 EVVSKSSIEVTSLRTLDIDSRASFRSFKKTCHM------NLFQLRTLNLDRCCCHPPKFV 600
Query: 601 GKMKHLRYVNLSNISIHFLPKFFTKLYHLETLILRNCYLLRGLPGDIKNLINLRHLDIMH 660
K+KHLRY+NLS +++ FLP T LY+LETLILR C LR LP DI NLINLRHLDI
Sbjct: 601 DKLKHLRYLNLSGLNVTFLPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLDIYD 660
Query: 661 CNSNNWTHMPIGLGSMTSLQTMNLFVLGKNKGSKLSELNGLNSLRGSLKIRHLQFCKSSD 720
C+S THMP GLG MTSLQTM++FVLGKNKG LS LNGL SLRG L I+ LQFC ++D
Sbjct: 661 CSS--LTHMPKGLGGMTSLQTMSMFVLGKNKGGDLSALNGLKSLRGLLCIKGLQFCTTAD 720
Query: 721 LENAKYLEEKFGIQKLELHWDRESKIDNALDD---EDEKVLEGLKPHPNLHKIHIEGFKG 780
L+N YL+E +GIQKLELHWD + ++ALDD +DE VLEGLKPH N+ K+ I+G++G
Sbjct: 721 LKNVSYLKEMYGIQKLELHWDIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYRG 780
Query: 781 VKLCNWLSFDSISSLVTIKLRNCEKLQHLPRFDQFPFLKHLHLEELSNIEYIDNNNFVSS 840
+KLC+W S + + LV+I+L +CEKL+HLP+FDQF +LKHL L L NIEYID+ N VSS
Sbjct: 781 MKLCDWFSSNFLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYLPNIEYIDSGNSVSS 840
Query: 841 STNFFPSLEKLSIISMPNLKGWWKGEMVTTNIIVGEDSSSTNVFRSLSSLSI-------S 900
ST FFPSLEKL I SMP LKGWWKGE+ S T + LS L I S
Sbjct: 841 STTFFPSLEKLRIESMPKLKGWWKGEI----------SFPTTILHQLSELCIFYCPLLAS 900
Query: 901 EMDFEFLPHDLFCNMT-HLKSLHISHCKNLKMSSDHDDVIWKELESLHSLKLTFIPKLEY 960
L C ++ L + I +L S L L L++ I LE+
Sbjct: 901 IPQHPSLESLRICGVSVQLFQMVIRMATDLSEHSSSSST----LSKLSFLEIGTI-DLEF 960
Query: 961 LP-KGFQYMTAVEHLILADCPNLVCIEG----------IEHLTSLSSLTIFNCPSLTSLP 1020
LP + F MT +E LI+ C +L + L++L +L + + L P
Sbjct: 961 LPVELFCNMTHLESLIIERCKSLQMSSPHPVDEDNDVVWKKLSNLRTLRLESILKLEYFP 1020
Query: 1021 QEMGQLTSLSSLTIIDCPNLTSLPQEMGQLTSLSSLGIFRCPNLTSLPQEMDQVTSLSSL 1080
+ + +TSL +L + +C NL S + +G+L SLS L I RCPNL L +++ + SLS L
Sbjct: 1021 KSLKYITSLETLKLSNCENLVS-TEGIGELISLSHLEIDRCPNLPILSEDVGDLISLSHL 1080
Query: 1081 TIDDITNLISLPQEMRQLTSLSSLTIIDCPNLTSLPQEM----GQLTSLSSLIIIDCPNL 1101
I + L SL + + +LTSLSSL + DCPNL SLPQE L L I++CP L
Sbjct: 1081 LIWNCPKLTSLSEGITRLTSLSSLCLEDCPNLVSLPQEFLHHHSSLPGGRFLRILNCPKL 1114
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
RGA3_SOLBU | 1.7e-151 | 35.53 | Putative disease resistance protein RGA3 OS=Solanum bulbocastanum GN=RGA3 PE=2 S... | [more] |
RGA2_SOLBU | 2.2e-146 | 35.38 | Disease resistance protein RGA2 OS=Solanum bulbocastanum GN=RGA2 PE=1 SV=1 | [more] |
RGA4_SOLBU | 1.1e-145 | 34.57 | Putative disease resistance protein RGA4 OS=Solanum bulbocastanum GN=RGA4 PE=2 S... | [more] |
RGA1_SOLBU | 5.2e-140 | 34.30 | Putative disease resistance protein RGA1 OS=Solanum bulbocastanum GN=RGA1 PE=2 S... | [more] |
R13L1_ARATH | 1.4e-113 | 30.74 | Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana GN=RPPL... | [more] |
Match Name | E-value | Identity | Description | |
A0A0A0M1L7_CUCSA | 1.0e-227 | 64.14 | Uncharacterized protein OS=Cucumis sativus GN=Csa_1G572370 PE=3 SV=1 | [more] |
A0A0A0KIT2_CUCSA | 6.5e-214 | 56.52 | Uncharacterized protein OS=Cucumis sativus GN=Csa_6G375730 PE=3 SV=1 | [more] |
A0A0D2NDD9_GOSRA | 3.0e-211 | 40.26 | Uncharacterized protein OS=Gossypium raimondii GN=B456_005G155200 PE=3 SV=1 | [more] |
U5FEY9_POPTR | 4.0e-211 | 40.43 | Uncharacterized protein (Fragment) OS=Populus trichocarpa GN=POPTR_0019s10290g P... | [more] |
A0A061EA34_THECC | 7.5e-210 | 41.03 | LRR and NB-ARC domains-containing disease resistance protein, putative OS=Theobr... | [more] |
Match Name | E-value | Identity | Description | |
AT3G14470.1 | 8.1e-115 | 30.74 | NB-ARC domain-containing disease resistance protein | [more] |
AT3G14460.1 | 2.5e-100 | 30.75 | LRR and NB-ARC domains-containing disease resistance protein | [more] |
AT3G46730.1 | 2.2e-67 | 28.14 | NB-ARC domain-containing disease resistance protein | [more] |
AT3G46530.1 | 1.0e-64 | 28.24 | NB-ARC domain-containing disease resistance protein | [more] |
AT3G07040.1 | 5.5e-63 | 28.59 | NB-ARC domain-containing disease resistance protein | [more] |