Lsi05G019430 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi05G019430
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionPectinesterase
Locationchr05 : 26597113 .. 26600159 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCCTTTTCCTTCGCTACCTTTCTTCTCCTCTTCTTACCCCTGGCAGTGGTGGCTTCCTCTAATTCTTCGTTGCCCGAGCTGGACCCGGTTCGATTGGCCCAGGCCGGGGTTAGGGAAGCCAGAATCGGAATAGCTAATTTAAAACGACAACGGTTTGGACGTCGGAATCTGCAGGCCGCACTTGCCGATTGCGCTACGCTCTACGAGGAGGCCGACTGGAGGCTCGCCGGGATGTTGGTTGGAGAGAATTATACGGCGGAAGATGCTCGGACGTGGGTTAGCGCCGCCGTCGCTAACCATCGGAGTTGCTTGGATGGCTTGGAAGAGGTCGGCGCTGCGGCGGCGGTGGATGGGAATAACTTGACCGTGTTGCTCACCGGAGCTCTGCATTTATACGACAAGATTGCAGCCGTGGAGCAGCGGAATGGTAAGCACCTGCTACCCACGTTGCAATTATCATTCCATATTCCAACTTATTTATATATTGATTTTTTAAAAAATATATTTTTATGTTTAAATCCTCAATTGGTATAGCAAATTTAATATCATATCATTTAATAATATAAAAAATAAAATAGTTTAATTTTGATATATACTATCAACTTGAAAACTAGAGGTTTATCACTCACCAAATTGACAAAAAAAAAAAAAAAAAAAAAAAAATTAAGAACAAAAATTAAATAATTTAAACTCTAGCAATATGGTTTTTAGTTTTTAATTTTTGAAAAGCAAGTTTACTTTTTTTTTTACATACTTTTTATTAACATTTTTAAAAACCAGTCAGTAACTCAAAATTCTGGGTTCAAATTGTAAAAAGAAAATAGCTCAAAAAATTACTAGACGGGGTGAGAGGAATCTTTCTGCCCCTCTTAAAAACTTGTATTTATACTTTTAATCAATTTTAAAGACTAAATCTAAGGTTTATTAAAAATAAATAATAACAACTATGGATTTAACCCACCATCCTTAGGTTTTTTAATTTTTTTTGGATGTTCCCACCACTACTATGTCAACCCATGATAGTTGATATTAATTAGATATTTGAAAGTTTAGGATGGGAAGTAGTGGTGAAACAGAAATGGAGAGAAAACAGAGGAATCAACTTGGCGAAATGGGATACGGCCACGTCGAAGGCGGATTATGTGGTGGCGAAGGATGGCTCCGGCACCCACACAACCATAAACGGCGCGGTGGCGGCTCTGGCGAGGACTGGACGGAGACGCCGGGGGGAGAGAATTGTGATATACGTGAAAGCCGGAGTTTACAAGGAGAATGTGGAAATTGGAATACAGTTGAATAACGTCATGGTTGTCGGCGACGGCGCAGACAAAACCATCGTCACCGGTAGCCGCAACGTGCCCGACGGCGCCACCACTTATAACTCAGCTACATTTGGTGAGCCCAAACAACAGATATTTTTCAATAATCAAATGCTCGACAGACCTAAGCTTTTTACTCAACTAGGCCTACAAAATTAAACAAAAAGGTTATGATAATACTTAATCTATTGAATTTAATTTTGATTAAAAGGAGAATCTCGCATTTAATTAAATTTAATCTTACTAACAAAAAAATAATAATAAAAGATAACTATATAATATAATAAGATAATAATTATAAAAATAGTGATATTATATTTCTTTAAAAACTAAGAAATGAATTTGTAAAAAAAATAATAAAAGAAATCAAAACAATTGTCACTAGTGGGTGGGTTCATTCCAATTTTCATTTTTATTAAAAAGAAAAAAAAAAACTACCTTATTCCCTTAAATATTTTAAAAACCATTATTTTCACATTTATTTAACCACCCTAAATATTTAAATACTAAGATTTAAGAGAAGATCAATTGAGCTTTTACCATAGAAATAAAATTAATCTAGTAGAATGCAATTAAACGTAATATTGAATACTTAAATATTCTAATACAATATTACTTAAATATTCTAATACCATGTTTATAAAATATACGAGTTATTTCACTCTCTTCTCAATTAATTTTGAGGCTTTCTTCAAGTAAGAAAAAAAAAACAATTAAAAGGTCCCAGGAAAAAAAATAATAATTAAAATTTGGCAAATGCTCTCACTAATTACTTGACTTTTTTTCTTTACTATAGCAAAGAAAAAATTTGTAATTTTCTTTTGTTTTTTAATTCTTTGTTTTCGAAAATAAAAACATGAACCTTTATAGCTTCTATTATTTAGGTAATTTTTTCTTTGTTTAAAAGTACACATAAAAACTGAAAATTAAAAAATATATATATATATTGAGATGAAACGAAACAGTAAAACTAATTTGAAATATGAAAACTTACCGAAATAAAAAATTTGATTGAACTTTCGTACCGGAGCAGGTGTTTCCGGCGACGGATTTTGGGCGAGGGACATAACATTCGAGAACACCGCTGGACCGGAAAAGCAACAGGCGGTGGCGCTACGGCTGAATTCCGACCTTGCCGTCGTATACCGGTGCGTAATAAAAGGCTATCAGGACACCCTCTTCCTCCACTCCCTCCGCCAATTCTACCGCGACTGCCAAATATACGGCACCGTCGATTTCATCTTCGGAAACTCCGCCGCCGTCCTCCAGAACTGCGAAATCTTCGTACGACGGCCGATTCGCCACCAGGCCAACATGATCACCGCCCAAGGTCGCGATGATCCGGCCGAGGCCACCGGAATTTCCATCCTCAACTCCAGAGTCCGGCCGACGCCGGATTTCGCTCACGTTAAGGCTCAGTACAGGACCTACCTCGGCCGCCCCTGGAAGCGGTTTTCGAGGACGGTGGTTATGGCGACTGATTTGGACGGATTGGTCGACCCGAAAGGGTGGGGAGAATGGAGGGGGGATTTTGCGATTTCGACTCTGTTTTATGCAGAGTTTAAGAACAGGGGCAGCGGTTCTTCGACGAGATTTAGGGTTAATTGGCCGGGATTTCACGTCCTCCGGAGTTCGGAGGAGGCGCGGCCTTTCACGGTGGCTGAATTTCTTCACGGCGGCGCTTGGATTCCGGCTACTGGCGTACCCTTCGAGGCGGGAGTTTGA

mRNA sequence

ATGCCCTTTTCCTTCGCTACCTTTCTTCTCCTCTTCTTACCCCTGGCAGTGGTGGCTTCCTCTAATTCTTCGTTGCCCGAGCTGGACCCGGTTCGATTGGCCCAGGCCGGGGTTAGGGAAGCCAGAATCGGAATAGCTAATTTAAAACGACAACGGTTTGGACGTCGGAATCTGCAGGCCGCACTTGCCGATTGCGCTACGCTCTACGAGGAGGCCGACTGGAGGCTCGCCGGGATGTTGGTTGGAGAGAATTATACGGCGGAAGATGCTCGGACGTGGGTTAGCGCCGCCGTCGCTAACCATCGGAGTTGCTTGGATGGCTTGGAAGAGGTCGGCGCTGCGGCGGCGGTGGATGGGAATAACTTGACCGTGTTGCTCACCGGAGCTCTGCATTTATACGACAAGATTGCAGCCGTGGAGCAGCGGAATGGTAAGCACCTGCTACCCACAGGAATCAACTTGGCGAAATGGGATACGGCCACGTCGAAGGCGGATTATGTGGTGGCGAAGGATGGCTCCGGCACCCACACAACCATAAACGGCGCGGTGGCGGCTCTGGCGAGGACTGGACGGAGACGCCGGGGGGAGAGAATTGTGATATACGTGAAAGCCGGAGTTTACAAGGAGAATGTGGAAATTGGAATACAGTTGAATAACGTCATGGTTGTCGGCGACGGCGCAGACAAAACCATCGTCACCGGTAGCCGCAACGTGCCCGACGGCGCCACCACTTATAACTCAGCTACATTTGGTGTTTCCGGCGACGGATTTTGGGCGAGGGACATAACATTCGAGAACACCGCTGGACCGGAAAAGCAACAGGCGGTGGCGCTACGGCTGAATTCCGACCTTGCCGTCGTATACCGGTGCGTAATAAAAGGCTATCAGGACACCCTCTTCCTCCACTCCCTCCGCCAATTCTACCGCGACTGCCAAATATACGGCACCGTCGATTTCATCTTCGGAAACTCCGCCGCCGTCCTCCAGAACTGCGAAATCTTCGTACGACGGCCGATTCGCCACCAGGCCAACATGATCACCGCCCAAGGTCGCGATGATCCGGCCGAGGCCACCGGAATTTCCATCCTCAACTCCAGAGTCCGGCCGACGCCGGATTTCGCTCACGTTAAGGCTCAGTACAGGACCTACCTCGGCCGCCCCTGGAAGCGGTTTTCGAGGACGGTGGTTATGGCGACTGATTTGGACGGATTGGTCGACCCGAAAGGGTGGGGAGAATGGAGGGGGGATTTTGCGATTTCGACTCTGTTTTATGCAGAGTTTAAGAACAGGGGCAGCGGTTCTTCGACGAGATTTAGGGTTAATTGGCCGGGATTTCACGTCCTCCGGAGTTCGGAGGAGGCGCGGCCTTTCACGGTGGCTGAATTTCTTCACGGCGGCGCTTGGATTCCGGCTACTGGCGTACCCTTCGAGGCGGGAGTTTGA

Coding sequence (CDS)

ATGCCCTTTTCCTTCGCTACCTTTCTTCTCCTCTTCTTACCCCTGGCAGTGGTGGCTTCCTCTAATTCTTCGTTGCCCGAGCTGGACCCGGTTCGATTGGCCCAGGCCGGGGTTAGGGAAGCCAGAATCGGAATAGCTAATTTAAAACGACAACGGTTTGGACGTCGGAATCTGCAGGCCGCACTTGCCGATTGCGCTACGCTCTACGAGGAGGCCGACTGGAGGCTCGCCGGGATGTTGGTTGGAGAGAATTATACGGCGGAAGATGCTCGGACGTGGGTTAGCGCCGCCGTCGCTAACCATCGGAGTTGCTTGGATGGCTTGGAAGAGGTCGGCGCTGCGGCGGCGGTGGATGGGAATAACTTGACCGTGTTGCTCACCGGAGCTCTGCATTTATACGACAAGATTGCAGCCGTGGAGCAGCGGAATGGTAAGCACCTGCTACCCACAGGAATCAACTTGGCGAAATGGGATACGGCCACGTCGAAGGCGGATTATGTGGTGGCGAAGGATGGCTCCGGCACCCACACAACCATAAACGGCGCGGTGGCGGCTCTGGCGAGGACTGGACGGAGACGCCGGGGGGAGAGAATTGTGATATACGTGAAAGCCGGAGTTTACAAGGAGAATGTGGAAATTGGAATACAGTTGAATAACGTCATGGTTGTCGGCGACGGCGCAGACAAAACCATCGTCACCGGTAGCCGCAACGTGCCCGACGGCGCCACCACTTATAACTCAGCTACATTTGGTGTTTCCGGCGACGGATTTTGGGCGAGGGACATAACATTCGAGAACACCGCTGGACCGGAAAAGCAACAGGCGGTGGCGCTACGGCTGAATTCCGACCTTGCCGTCGTATACCGGTGCGTAATAAAAGGCTATCAGGACACCCTCTTCCTCCACTCCCTCCGCCAATTCTACCGCGACTGCCAAATATACGGCACCGTCGATTTCATCTTCGGAAACTCCGCCGCCGTCCTCCAGAACTGCGAAATCTTCGTACGACGGCCGATTCGCCACCAGGCCAACATGATCACCGCCCAAGGTCGCGATGATCCGGCCGAGGCCACCGGAATTTCCATCCTCAACTCCAGAGTCCGGCCGACGCCGGATTTCGCTCACGTTAAGGCTCAGTACAGGACCTACCTCGGCCGCCCCTGGAAGCGGTTTTCGAGGACGGTGGTTATGGCGACTGATTTGGACGGATTGGTCGACCCGAAAGGGTGGGGAGAATGGAGGGGGGATTTTGCGATTTCGACTCTGTTTTATGCAGAGTTTAAGAACAGGGGCAGCGGTTCTTCGACGAGATTTAGGGTTAATTGGCCGGGATTTCACGTCCTCCGGAGTTCGGAGGAGGCGCGGCCTTTCACGGTGGCTGAATTTCTTCACGGCGGCGCTTGGATTCCGGCTACTGGCGTACCCTTCGAGGCGGGAGTTTGA

Protein sequence

MPFSFATFLLLFLPLAVVASSNSSLPELDPVRLAQAGVREARIGIANLKRQRFGRRNLQAALADCATLYEEADWRLAGMLVGENYTAEDARTWVSAAVANHRSCLDGLEEVGAAAAVDGNNLTVLLTGALHLYDKIAAVEQRNGKHLLPTGINLAKWDTATSKADYVVAKDGSGTHTTINGAVAALARTGRRRRGERIVIYVKAGVYKENVEIGIQLNNVMVVGDGADKTIVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPEKQQAVALRLNSDLAVVYRCVIKGYQDTLFLHSLRQFYRDCQIYGTVDFIFGNSAAVLQNCEIFVRRPIRHQANMITAQGRDDPAEATGISILNSRVRPTPDFAHVKAQYRTYLGRPWKRFSRTVVMATDLDGLVDPKGWGEWRGDFAISTLFYAEFKNRGSGSSTRFRVNWPGFHVLRSSEEARPFTVAEFLHGGAWIPATGVPFEAGV
BLAST of Lsi05G019430 vs. Swiss-Prot
Match: PME36_ARATH (Probable pectinesterase/pectinesterase inhibitor 36 OS=Arabidopsis thaliana GN=PME36 PE=2 SV=2)

HSP 1 Score: 518.8 bits (1335), Expect = 6.1e-146
Identity = 267/510 (52.35%), Postives = 349/510 (68.43%), Query Frame = 1

Query: 9   LLLFLPLAVVASSNSSLPELDPVRLAQAGVREARIGIANLK----RQRFGRRNLQAALAD 68
           ++ FL +A V ++ S+  ELD + +A+  V EA+    ++              +  L++
Sbjct: 13  IMFFLAIAAVITA-SNTAELDVLEMARTAVVEAKTSFGSMAVTEATSEVAGSYYKLGLSE 72

Query: 69  CATLYEEADWRLAGMLVG-ENYTAEDARTWVSAAVANHRSCLDGL-EEVGAAAAVDGNNL 128
           C  LY+E++ RL+ ++V  EN+T ED RTW+S  +ANH +CLDGL ++      +  +N+
Sbjct: 73  CEKLYDESEARLSKLVVDHENFTVEDVRTWLSGVLANHHTCLDGLIQQRQGHKPLVHSNV 132

Query: 129 TVLLTGALHLYDKI---------AAVEQRNG----KHLLPTGINLA-------------- 188
           T +L  AL  Y K              Q +G    KH  PT  N                
Sbjct: 133 TFVLHEALAFYKKSRGHMKKRLHGPARQGHGPTRPKH-RPTRPNHGPGRSHHGPSRPNQN 192

Query: 189 -----KWDTATSKADYVVAKDGSGTHTTINGAVAALARTGRRRRGERIVIYVKAGVYKEN 248
                 W+  +S+AD+VVA+DGS TH TIN A+AA++R G+ R   R++IY+KAGVY E 
Sbjct: 193 GGMLVSWNPTSSRADFVVARDGSATHRTINQALAAVSRMGKSRL-NRVIIYIKAGVYNEK 252

Query: 249 VEIGIQLNNVMVVGDGADKTIVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGP 308
           +EI   + N+M+VGDG D+TIVT +RNVPDG+TTY SATFGVSGDGFWARDITFENTAGP
Sbjct: 253 IEIDRHMKNIMLVGDGMDRTIVTNNRNVPDGSTTYGSATFGVSGDGFWARDITFENTAGP 312

Query: 309 EKQQAVALRLNSDLAVVYRCVIKGYQDTLFLHSLRQFYRDCQIYGTVDFIFGNSAAVLQN 368
            K QAVALR++SDL++ YRC  KGYQDTLF HSLRQFYRDC IYGT+DFIFG++AAV QN
Sbjct: 313 HKHQAVALRVSSDLSLFYRCSFKGYQDTLFTHSLRQFYRDCHIYGTIDFIFGDAAAVFQN 372

Query: 369 CEIFVRRPIRHQANMITAQGRDDPAEATGISILNSRVRPTPDFAHVKAQYRTYLGRPWKR 428
           C+IFVRRP+ HQ NMITAQGRDDP   +GISI +SR+R  P+F  VK ++++YLGRPWK+
Sbjct: 373 CDIFVRRPMDHQGNMITAQGRDDPHTNSGISIQHSRIRAAPEFEAVKGRFKSYLGRPWKK 432

Query: 429 FSRTVVMATDLDGLVDPKGWGEWRGDFAISTLFYAEFKNRGSGSSTRFRVNWPGFHVLRS 481
           +SRTV + TD+D L+DP+GW EW G +A+STL+Y EF N G+G+ T  RVNWPGFHVLR 
Sbjct: 433 YSRTVFLKTDIDELIDPRGWREWSGSYALSTLYYGEFMNTGAGAGTGRRVNWPGFHVLRG 492

BLAST of Lsi05G019430 vs. Swiss-Prot
Match: PME2_CITSI (Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 1.6e-109
Identity = 217/428 (50.70%), Postives = 277/428 (64.72%), Query Frame = 1

Query: 59  QAALADCATLYEEADWRLAGMLVGE-NYTAEDARTWVSAAVANHRSCLDGLEEVGAAAAV 118
           +AA  DC  LYE    +L          T  D +TW+S A+ N  +C   LE++G    V
Sbjct: 92  KAAWEDCRELYELTVLKLNQTSNSSPGCTKVDKQTWLSTALTNLETCRASLEDLGVPEYV 151

Query: 119 D---GNNLTVLLTGALHLYDKIAAVEQRNGKHLLPTGINLA--KWDTATSKADYVVAKDG 178
                NN+T L++  L L +K+   E  + K   PT +     K    T +A+ VVA+DG
Sbjct: 152 LPLLSNNVTKLISNTLSL-NKVPYNEP-SYKDGFPTWVKPGDRKLLQTTPRANIVVAQDG 211

Query: 179 SGTHTTINGAVAALARTGRRRRGERIVIYVKAGVYKENVEIGIQLNNVMVVGDGADKTIV 238
           SG   TI  AVAA +R G    G R VIY+KAG Y EN+E+  +L N+M VGDG  KTI+
Sbjct: 212 SGNVKTIQEAVAAASRAG----GSRYVIYIKAGTYNENIEV--KLKNIMFVGDGIGKTII 271

Query: 239 TGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPEKQQAVALRLNSDLAVVYRCVI 298
           TGS++V  GATT+ SAT  V GD F ARDIT  NTAGP   QAVALR  SDL+V YRC  
Sbjct: 272 TGSKSVGGGATTFKSATVAVVGDNFIARDITIRNTAGPNNHQAVALRSGSDLSVFYRCSF 331

Query: 299 KGYQDTLFLHSLRQFYRDCQIYGTVDFIFGNSAAVLQNCEIFVRRPIRHQANMITAQGRD 358
           +GYQDTL++HS RQFYR+C IYGTVDFIFGN+A VLQNC IF R+P  ++ N +TAQGR 
Sbjct: 332 EGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQNCNIFARKP-PNRTNTLTAQGRT 391

Query: 359 DPAEATGISILNSRVRPTPDFAHVKAQYRTYLGRPWKRFSRTVVMATDLDGLVDPKGWGE 418
           DP ++TGI I N RV    D   V++  +T+LGRPWK++SRTV + T LD L++P GW E
Sbjct: 392 DPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGRPWKQYSRTVYIKTFLDSLINPAGWME 451

Query: 419 WRGDFAISTLFYAEFKNRGSGSSTRFRVNWPGFHVLRSSEEARPFTVAEFLHGGAWIPAT 478
           W GDFA++TL+YAE+ N G GSST  RV W G+HVL S  +   FTV  F+ G +W+PAT
Sbjct: 452 WSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQVSQFTVGNFIAGNSWLPAT 510

Query: 479 GVPFEAGV 481
            VPF +G+
Sbjct: 512 NVPFTSGL 510

BLAST of Lsi05G019430 vs. Swiss-Prot
Match: PME59_ARATH (Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana GN=PME59 PE=2 SV=1)

HSP 1 Score: 388.3 bits (996), Expect = 1.2e-106
Identity = 208/441 (47.17%), Postives = 275/441 (62.36%), Query Frame = 1

Query: 59  QAALADCATLYEEADWRLAGMLVG--------ENYTAEDARTWVSAAVANHRSCLDGLEE 118
           QA LADC  LY +   +L   L G        ++ T  DA+TW+S A+ N  +C  G  +
Sbjct: 98  QAVLADCIDLYGDTIMQLNRTLHGVSPKAGAAKSCTDFDAQTWLSTALTNTETCRRGSSD 157

Query: 119 VGAA----AAVDGNNLTVLLTGALHLYDKIAAVEQRNGKHLLPTGIN--LAKWDTA---T 178
           +         V    ++ L++  L +   +     +        G    L++ D      
Sbjct: 158 LNVTDFITPIVSNTKISHLISNCLAVNGALLTAGNKGNTTANQKGFPTWLSRKDKRLLRA 217

Query: 179 SKADYVVAKDGSGTHTTINGAVAALARTGRRRRGERIVIYVKAGVYKENVEIGIQLNNVM 238
            +A+ VVAKDGSG   T+  A+    R  R+    R VIYVK G+Y+EN+ + +  +++M
Sbjct: 218 VRANLVVAKDGSGHFNTVQAAIDVAGR--RKVTSGRFVIYVKRGIYQENINVRLNNDDIM 277

Query: 239 VVGDGADKTIVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPEKQQAVALRLN 298
           +VGDG   TI+TG R+V  G TTYNSAT G+ G  F A+ ITF NTAGP K QAVALR +
Sbjct: 278 LVGDGMRSTIITGGRSVQGGYTTYNSATAGIEGLHFIAKGITFRNTAGPAKGQAVALRSS 337

Query: 299 SDLAVVYRCVIKGYQDTLFLHSLRQFYRDCQIYGTVDFIFGNSAAVLQNCEIFVRRPIRH 358
           SDL++ Y+C I+GYQDTL +HS RQFYR+C IYGTVDFIFGN+AAV QNC I  RRP++ 
Sbjct: 338 SDLSIFYKCSIEGYQDTLMVHSQRQFYRECYIYGTVDFIFGNAAAVFQNCLILPRRPLKG 397

Query: 359 QANMITAQGRDDPAEATGISILNSRVRPTPDFAHVKAQYRTYLGRPWKRFSRTVVMATDL 418
           QAN+ITAQGR DP + TGISI NSR+ P PD   V    +TY+GRPW +FSRTVV+ T L
Sbjct: 398 QANVITAQGRADPFQNTGISIHNSRILPAPDLKPVVGTVKTYMGRPWMKFSRTVVLQTYL 457

Query: 419 DGLVDPKGWGEW--RGDFAISTLFYAEFKNRGSGSSTRFRVNWPGFHVLRSSEEARPFTV 478
           D +V P GW  W     F + TLFYAE+KN G  SSTR+RV+W GFHVL  + +A  FTV
Sbjct: 458 DNVVSPVGWSPWIEGSVFGLDTLFYAEYKNTGPASSTRWRVSWKGFHVLGRASDASAFTV 517

Query: 479 AEFLHGGAWIPATGVPFEAGV 481
            +F+ G AW+P TG+PF +G+
Sbjct: 518 GKFIAGTAWLPRTGIPFTSGL 536

BLAST of Lsi05G019430 vs. Swiss-Prot
Match: PME17_ARATH (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana GN=PME17 PE=2 SV=2)

HSP 1 Score: 387.1 bits (993), Expect = 2.8e-106
Identity = 218/429 (50.82%), Postives = 274/429 (63.87%), Query Frame = 1

Query: 59  QAALADCATLYEEADWRL-AGMLVGENYTAEDARTWVSAAVANHRSCLDGLEEVGAAAAV 118
           +AA  DC  LY+    ++   M      +  DA+TW+S A+ N  +C  G  E+G    V
Sbjct: 94  KAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQTWLSTALTNLDTCRAGFLELGVTDIV 153

Query: 119 ---DGNNLTVLLTGALHLYDKIAAVEQRNGKHLLPTGINLAK---WDTATSKADYVVAKD 178
                NN++ LL   L + +K+        K   P+ +         ++T K + VVAKD
Sbjct: 154 LPLMSNNVSNLLCNTLAI-NKVPFNYTPPEKDGFPSWVKPGDRKLLQSSTPKDNAVVAKD 213

Query: 179 GSGTHTTINGAVAALARTGRRRRGERIVIYVKAGVYKENVEIGIQLNNVMVVGDGADKTI 238
           GSG   TI  A+ A + +GR       VIYVK GVY EN+EI  +  NVM+ GDG  KTI
Sbjct: 214 GSGNFKTIKEAIDAASGSGR------FVIYVKQGVYSENLEI--RKKNVMLRGDGIGKTI 273

Query: 239 VTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPEKQQAVALRLNSDLAVVYRCV 298
           +TGS++V  G TT+NSAT    GDGF AR ITF NTAG   +QAVALR  SDL+V Y+C 
Sbjct: 274 ITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNTAGASNEQAVALRSGSDLSVFYQCS 333

Query: 299 IKGYQDTLFLHSLRQFYRDCQIYGTVDFIFGNSAAVLQNCEIFVRRPIRHQANMITAQGR 358
            + YQDTL++HS RQFYRDC +YGTVDFIFGN+AAVLQNC IF RRP R + N ITAQGR
Sbjct: 334 FEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCNIFARRP-RSKTNTITAQGR 393

Query: 359 DDPAEATGISILNSRVRPTPDFAHVKAQYRTYLGRPWKRFSRTVVMATDLDGLVDPKGWG 418
            DP + TGI I NSRV    D   V    +TYLGRPW+++SRTV M T LD L+DP+GW 
Sbjct: 394 SDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWRQYSRTVFMKTSLDSLIDPRGWL 453

Query: 419 EWRGDFAISTLFYAEFKNRGSGSSTRFRVNWPGFHVLRSSEEARPFTVAEFLHGGAWIPA 478
           EW G+FA+ TLFYAEF+N G G+ST  RV WPGF VL S+ EA  FTV  FL GG+WIP+
Sbjct: 454 EWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGSASEASKFTVGTFLAGGSWIPS 511

Query: 479 TGVPFEAGV 481
           + VPF +G+
Sbjct: 514 S-VPFTSGL 511

BLAST of Lsi05G019430 vs. Swiss-Prot
Match: PME1_FICPW (Pectinesterase/pectinesterase inhibitor OS=Ficus pumila var. awkeotsang PE=1 SV=1)

HSP 1 Score: 384.8 bits (987), Expect = 1.4e-105
Identity = 206/454 (45.37%), Postives = 276/454 (60.79%), Query Frame = 1

Query: 46  ANLKRQRFGRRNLQAALADCATLYEEADWRLA---GMLVGENYTA---EDARTWVSAAVA 105
           AN   +R      + AL DCA L + +  R+     +L  +N T    ED   W+S  + 
Sbjct: 94  ANDASRRINNPQERTALLDCAELMDLSKERVVDSISILFHQNLTTRSHEDLHVWLSGVLT 153

Query: 106 NHRSCLDGLEEVGA--AAAVDGNNLTVLLTGALHLYDKIAAVEQRNGKHLLPTGINLAKW 165
           NH +CLDGLEE        +  ++L  L+  A         +       + P   N   W
Sbjct: 154 NHVTCLDGLEEGSTDYIKTLMESHLNELILRARTSLAIFVTLFPAKSNVIEPVTGNFPTW 213

Query: 166 DTATSKA-----------DYVVAKDGSGTHTTINGAVAALARTGRRRRGERIVIYVKAGV 225
            TA  +            D VVAKDGSG + T+N AVAA+    ++R    +++ V+ G+
Sbjct: 214 VTAGDRRLLQTLGKDIEPDIVVAKDGSGDYETLNEAVAAIPDNSKKR----VIVLVRTGI 273

Query: 226 YKENVEIGIQLNNVMVVGDGADKTIVTGSRNVPDGATTYNSATFGVSGDGFWARDITFEN 285
           Y+ENV+ G Q  NVM+VG+G D TI+TGSRNV DG+TT++SAT    GDGF A+DI F+N
Sbjct: 274 YEENVDFGYQKKNVMLVGEGMDYTIITGSRNVVDGSTTFDSATVAAVGDGFIAQDICFQN 333

Query: 286 TAGPEKQQAVALRLNSDLAVVYRCVIKGYQDTLFLHSLRQFYRDCQIYGTVDFIFGNSAA 345
           TAGPEK QAVALR+ +D  V+ RC I  YQDTL+ H+ RQFYRD  I GTVDFIFGN+A 
Sbjct: 334 TAGPEKYQAVALRIGADETVINRCRIDAYQDTLYPHNYRQFYRDRNITGTVDFIFGNAAV 393

Query: 346 VLQNCEIFVRRPIRHQANMITAQGRDDPAEATGISILNSRVRPTPDFAHVKAQYRTYLGR 405
           V QNC +  R+ ++ Q N ITAQGR DP + TG SI N  +  + D   V+  +++YLGR
Sbjct: 394 VFQNCNLIPRKQMKGQENTITAQGRTDPNQNTGTSIQNCEIFASADLEPVEDTFKSYLGR 453

Query: 406 PWKRFSRTVVMATDLDGLVDPKGWGEWRGDFAISTLFYAEFKNRGSGSSTRFRVNWPGFH 465
           PWK +SRTVVM + +  ++DP GW EW  DFA+ TLFY E++N G GS T  RV WPG+H
Sbjct: 454 PWKEYSRTVVMESYISDVIDPAGWLEWDRDFALKTLFYGEYRNGGPGSGTSERVKWPGYH 513

Query: 466 VLRSSEEARPFTVAEFLHGGAWIPATGVPFEAGV 481
           V+ S E A  FTVAE + GG+W+ +TGV + AG+
Sbjct: 514 VITSPEVAEQFTVAELIQGGSWLGSTGVDYTAGL 543

BLAST of Lsi05G019430 vs. TrEMBL
Match: A0A0A0L3U7_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_3G126790 PE=4 SV=1)

HSP 1 Score: 810.8 bits (2093), Expect = 8.7e-232
Identity = 413/489 (84.46%), Postives = 435/489 (88.96%), Query Frame = 1

Query: 1   MPFSFATFLLL-FLPLAVVASSNSSLPELDPVRLAQAGVREARIGIANLKRQRFGRRNLQ 60
           MPF F TFLLL FLP   VASSN SLP LD VRLAQ GVREA   I NLKR++FG  NLQ
Sbjct: 1   MPFPFTTFLLLLFLPF--VASSNFSLPALDSVRLAQTGVREAITEIGNLKRRQFGDWNLQ 60

Query: 61  AALADCATLYEEADWRLAGMLVGEN-YTAEDARTWVSAAVANHRSCLDGLEEVGAAAAVD 120
           AAL DCATLYEEA+WRLAGMLVGEN Y AED R WVSAA+ANHRSCLDGLEEV   AAVD
Sbjct: 61  AALRDCATLYEEAEWRLAGMLVGENKYRAEDGRMWVSAAMANHRSCLDGLEEVHDVAAVD 120

Query: 121 GNNLTVLLTGALHLYDKIAAVEQRNGKHLLPT-------GINLAKWDTATSKADYVVAKD 180
           GNNLTV+LTGALHLYDKIAAVE+RNG+  L         G NLA W+ ATSKA+YVVAKD
Sbjct: 121 GNNLTVMLTGALHLYDKIAAVEKRNGRKRLGKRKWRENRGTNLATWNPATSKANYVVAKD 180

Query: 181 GSGTHTTINGAVAALARTGRRRRGERIVIYVKAGVYKENVEIGIQLNNVMVVGDGADKTI 240
           GSGTH TIN AVAALAR+GR RRG RIVIYVKAGVY+ENVEIGIQL NVM+VGDG DKTI
Sbjct: 181 GSGTHRTINRAVAALARSGRTRRGGRIVIYVKAGVYRENVEIGIQLKNVMLVGDGIDKTI 240

Query: 241 VTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPEKQQAVALRLNSDLAVVYRCV 300
           VTGSRNVPDGATTYNSATFGVSGDGFW RDITFENTAGP KQQAVALRLNSDLAVVYRC 
Sbjct: 241 VTGSRNVPDGATTYNSATFGVSGDGFWVRDITFENTAGPGKQQAVALRLNSDLAVVYRCA 300

Query: 301 IKGYQDTLFLHSLRQFYRDCQIYGTVDFIFGNSAAVLQNCEIFVRRPIRHQANMITAQGR 360
           IKGYQDTLFLHSLRQFYRDC+IYGT+DFIFGNSAAVLQNCEIFVR+PI HQANMITAQGR
Sbjct: 301 IKGYQDTLFLHSLRQFYRDCKIYGTIDFIFGNSAAVLQNCEIFVRQPILHQANMITAQGR 360

Query: 361 DDPAEATGISILNSRVRPTPDFAHVKAQYRTYLGRPWKRFSRTVVMATDLDGLVDPKGWG 420
           DDPAEATG SILNSRV+P P+F+ VKAQYRTYLGRPWKRFSRTVV+ATDLDGL+DPKGWG
Sbjct: 361 DDPAEATGFSILNSRVQPAPEFSPVKAQYRTYLGRPWKRFSRTVVIATDLDGLIDPKGWG 420

Query: 421 EWRGDFAISTLFYAEFKNRGSGSSTRFRVNWPGFHVLRSSEEARPFTVAEFLHGGAWIPA 480
           EW GDFAISTLFYAEFKNRG GSSTRFRVNWPGFH+LRS+EEARPFTVAEFLHGG WIPA
Sbjct: 421 EWTGDFAISTLFYAEFKNRGDGSSTRFRVNWPGFHILRSAEEARPFTVAEFLHGGDWIPA 480

BLAST of Lsi05G019430 vs. TrEMBL
Match: A0A0D2PSW3_GOSRA (Pectinesterase OS=Gossypium raimondii GN=B456_008G113200 PE=4 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 2.7e-156
Identity = 285/482 (59.13%), Postives = 353/482 (73.24%), Query Frame = 1

Query: 7   TFLLLFLPLAVVASSNSSLPEL-DPVRLAQAGVREARIGIANLKRQRFGRRNLQ----AA 66
           TFLLLFL     A++ S L ++ + V++A+  V ++R  +A      +G  N        
Sbjct: 18  TFLLLFLLAMATATTGSHLEQVTEVVQMARTMVVQSR-NLAKASMSLYGLHNTMHYHGMG 77

Query: 67  LADCATLYEEADWRLAGMLVGENYTAEDARTWVSAAVANHRSCLDGL--EEVGAAAAVDG 126
           LADC  LY+E++ RL+ +L    YT +DARTW+S  +ANHRSC +GL  ++ G      G
Sbjct: 78  LADCIKLYDESESRLSVLLSSRGYTVDDARTWLSGVLANHRSCSEGLGQKDFGIQEHGQG 137

Query: 127 NNLTVLLTGALHLYD---KIAAVEQRNGKHLLPTGINLAKWDTATSKADYVVAKDGSGTH 186
            NLT L++ AL LY    K A VEQ         G  L  W+  TSKAD+VV+KDGSGTH
Sbjct: 138 RNLTSLVSAALALYTMHGKEAVVEQELMHRPRLGGKLLTSWNPTTSKADFVVSKDGSGTH 197

Query: 187 TTINGAVAALARTGRRRRGERIVIYVKAGVYKENVEIGIQLNNVMVVGDGADKTIVTGSR 246
            TIN AVAA+AR G  RR  R++IYVKAGVY E V+I   L N+M+VGDG DKTIVTG+R
Sbjct: 198 KTINDAVAAVARIGNNRR-PRVIIYVKAGVYNEKVDIDKNLKNIMLVGDGMDKTIVTGNR 257

Query: 247 NVPDGATTYNSATFGVSGDGFWARDITFENTAGPEKQQAVALRLNSDLAVVYRCVIKGYQ 306
           NVPDG+TTY SATFGVSGDGFWARD+TF NTAGP K QAVALR++SD +V YRC IKGYQ
Sbjct: 258 NVPDGSTTYGSATFGVSGDGFWARDVTFVNTAGPHKHQAVALRVSSDHSVFYRCSIKGYQ 317

Query: 307 DTLFLHSLRQFYRDCQIYGTVDFIFGNSAAVLQNCEIFVRRPIRHQANMITAQGRDDPAE 366
           D+LFLHSLRQFYRDC I+GT+D+IFG+++AVLQNC+IFV+RP+ HQ+NM+TAQGRDDP E
Sbjct: 318 DSLFLHSLRQFYRDCHIHGTIDYIFGDASAVLQNCDIFVKRPMDHQSNMVTAQGRDDPNE 377

Query: 367 ATGISILNSRVRPTPDFAHVKAQYRTYLGRPWKRFSRTVVMATDLDGLVDPKGWGEWRGD 426
            TGISI+ SRVRP PDF  VK  +++YLGRPWK++SRTV M TDLDGL+DPKGW EW G 
Sbjct: 378 NTGISIMESRVRPAPDFDSVKHLFKSYLGRPWKKYSRTVFMKTDLDGLIDPKGWNEWSGS 437

Query: 427 FAISTLFYAEFKNRGSGSSTRFRVNWPGFHVLRSSEEARPFTVAEFLHGGAWIPATGVPF 479
           FA+STL+YAE++N G+G+S   RV WPGFHV   +EEA PFTV  F+ G  WIP TGVPF
Sbjct: 438 FALSTLYYAEYRNTGNGASIGNRVKWPGFHVFNRAEEANPFTVNRFIQGELWIPETGVPF 497

BLAST of Lsi05G019430 vs. TrEMBL
Match: A0A078C450_BRANA (Pectinesterase OS=Brassica napus GN=BnaC08g30880D PE=4 SV=1)

HSP 1 Score: 540.8 bits (1392), Expect = 1.7e-150
Identity = 276/512 (53.91%), Postives = 351/512 (68.55%), Query Frame = 1

Query: 9   LLLFLPLAVVASSNSSLPELDPVRLAQAGVREARIGIANLKR----QRFGRRNLQAALAD 68
           +L FL  A VA S ++  ELD +  A+A V EAR    ++          R      L+D
Sbjct: 13  ILFFLATATVAISFNT-SELDVLERARASVVEARTRFGSMATVEATNEVARSYYSLGLSD 72

Query: 69  CATLYEEADWRLAGMLVG-ENYTAEDARTWVSAAVANHRSCLDGLEEVGAA-AAVDGNNL 128
           C  LY+E++ RL  ++V  EN+T ED RTW+S  +ANH +CLDGL++       +  +N+
Sbjct: 73  CEKLYDESEARLLNLVVAHENFTVEDVRTWLSGVLANHHTCLDGLDQSRQGDKPLVHSNI 132

Query: 129 TVLLTGALHLYDKIAAVEQRNGKHLLPT-------------------------------- 188
           TV+L  AL  Y K     ++   +  PT                                
Sbjct: 133 TVVLGEALAFYKKTRGRLKKRRPNHTPTRQHHAPTRPHHGPTRPNHNGPARPYHGPVRPN 192

Query: 189 --GINLAKWDTATSKADYVVAKDGSGTHTTINGAVAALARTGRRRRGERIVIYVKAGVYK 248
             G  L  WD   S+AD+VVAKDGS TH +I+ A+AA++R G+ R   R++IY+KAGVY 
Sbjct: 193 QSGGMLVSWDPTGSRADFVVAKDGSATHRSISEALAAVSRMGKSRT-NRVIIYIKAGVYN 252

Query: 249 ENVEIGIQLNNVMVVGDGADKTIVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTA 308
           E +EI   + N+M+VGDG D+TI+T +RNVPDG+TTY SATFGVSGDGFW+RD+TFENTA
Sbjct: 253 EKIEIDRHMKNIMLVGDGMDRTIITNNRNVPDGSTTYGSATFGVSGDGFWSRDMTFENTA 312

Query: 309 GPEKQQAVALRLNSDLAVVYRCVIKGYQDTLFLHSLRQFYRDCQIYGTVDFIFGNSAAVL 368
           GP K QAVALR+NSDL+V YRC  +GYQDTLF HSLRQFYRDC IYGT+DFIFG++AAV 
Sbjct: 313 GPHKHQAVALRVNSDLSVFYRCSFRGYQDTLFTHSLRQFYRDCHIYGTIDFIFGDAAAVF 372

Query: 369 QNCEIFVRRPIRHQANMITAQGRDDPAEATGISILNSRVRPTPDFAHVKAQYRTYLGRPW 428
           QNC+IFVRRP+ HQ NMITAQGRD+P E TGISI NSRVR +P+F  VK Q+++YLGRPW
Sbjct: 373 QNCDIFVRRPMDHQGNMITAQGRDNPHENTGISIQNSRVRASPEFEAVKGQFKSYLGRPW 432

Query: 429 KRFSRTVVMATDLDGLVDPKGWGEWRGDFAISTLFYAEFKNRGSGSSTRFRVNWPGFHVL 481
           K++SRTV + TDLDGL+DP+GW EWRGDFA+STL+Y EF N GSG+ T  RV+WPGFHVL
Sbjct: 433 KKYSRTVFLKTDLDGLIDPRGWREWRGDFALSTLYYGEFMNTGSGAGTSRRVSWPGFHVL 492

BLAST of Lsi05G019430 vs. TrEMBL
Match: B9RD91_RICCO (Pectinesterase OS=Ricinus communis GN=RCOM_1611150 PE=4 SV=1)

HSP 1 Score: 538.5 bits (1386), Expect = 8.3e-150
Identity = 278/475 (58.53%), Postives = 339/475 (71.37%), Query Frame = 1

Query: 17  VVASSNSSLPELDPVRLAQAGVREAR----IGIANLKRQRFGRRNLQAALADCATLYEEA 76
           +  SS   L EL+ ++ AQ    + R      +     Q F   N    L+DC  LY E+
Sbjct: 1   MATSSTFCLQELETIQTAQTQTLQVRKWLQSSLGGGLNQGFSY-NFGVPLSDCIKLYSES 60

Query: 77  DWRLAGMLVGE-NYTAEDARTWVSAAVANHRSCLDGLEEVGAAAAVD--GNNLTVLLTGA 136
           ++RL+ +L  E NYT  DARTW+S  +ANH++CLDGL E G         +NLT  L+ +
Sbjct: 61  EFRLSQLLASEKNYTHHDARTWLSGVLANHKTCLDGLSEKGFLENDHEMAHNLTFSLSKS 120

Query: 137 LHLYDK----IAAVEQRNGKHLLPTGINLAKWDTATSKADYVVAKDGSGTHTTINGAVAA 196
           L LY +    I     R   H    GI L  W+  TS+AD+VVA+DGSGTH TIN A+AA
Sbjct: 121 LALYSRGRRTINRGVPRRPIHNYNGGI-LTSWNPTTSQADFVVARDGSGTHRTINDALAA 180

Query: 197 LARTGRRRRGERIVIYVKAGVYKENVEIGIQLNNVMVVGDGADKTIVTGSRNVPDGATTY 256
           L+R G RR  +R++IYVKAGVY E VEI   + +VM VGDG DKTI+TGSRNVPDG++T+
Sbjct: 181 LSRLGTRRT-QRVIIYVKAGVYNEKVEIDHHIKDVMFVGDGIDKTIITGSRNVPDGSSTF 240

Query: 257 NSATFGVSGDGFWARDITFENTAGPEKQQAVALRLNSDLAVVYRCVIKGYQDTLFLHSLR 316
           +SATFGVSGDGFWARDITFENTAGP K QAVALR++SDL+V YRC  K YQDTLF+ SLR
Sbjct: 241 SSATFGVSGDGFWARDITFENTAGPHKHQAVALRVSSDLSVFYRCSFKAYQDTLFVLSLR 300

Query: 317 QFYRDCQIYGTVDFIFGNSAAVLQNCEIFVRRPIRHQANMITAQGRDDPAEATGISILNS 376
           QFYRDC IYGT+DFIFG++  V QNC+IFVRRP+ HQAN ITAQGRDDP E TGISI  S
Sbjct: 301 QFYRDCHIYGTIDFIFGDAPVVFQNCDIFVRRPMDHQANFITAQGRDDPNENTGISIQRS 360

Query: 377 RVRPTPDFAHVKAQYRTYLGRPWKRFSRTVVMATDLDGLVDPKGWGEWRGDFAISTLFYA 436
           RVR +PDF   K + R++LGRPWK++SRTV M TDLDGL+DP GWG W GDFA+STLFYA
Sbjct: 361 RVRASPDFMVAKNKIRSFLGRPWKKYSRTVFMQTDLDGLIDPMGWGAWHGDFALSTLFYA 420

Query: 437 EFKNRGSGSSTRFRVNWPGFHVLRSSEEARPFTVAEFLHGGAWIPATGVPFEAGV 481
           E+ N G+G+ST  RV WPGFHVL S ++A PFTV  F+ G +WIPATGVPF  G+
Sbjct: 421 EYMNTGNGASTSRRVKWPGFHVLSSPQQASPFTVTRFIQGESWIPATGVPFWVGI 472

BLAST of Lsi05G019430 vs. TrEMBL
Match: D7U7W0_VITVI (Pectinesterase OS=Vitis vinifera GN=VIT_15s0048g00500 PE=4 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 6.0e-148
Identity = 274/499 (54.91%), Postives = 344/499 (68.94%), Query Frame = 1

Query: 1   MPFSFATFLLLFLPLAVVASSNSSLPELDPVRLAQAGVREARIGIANLKRQRFGRRNLQA 60
           M +   TFLLL L   +  + N  L            VR AR G+  L+  R+ R +L+ 
Sbjct: 1   MAYWVTTFLLL-LSFTISVACNEELK----------AVRTARFGL--LQGGRWARGSLRL 60

Query: 61  -------------ALADCATLYEEADWRLAGMLVGENYTAEDARTWVSAAVANHRSCLDG 120
                        AL DC  LYEE++ RL  +L GE    +DARTW+S+A+A+HR+CLDG
Sbjct: 61  HGLNLVDSGYVGEALGDCVKLYEESESRLTRLLSGETRNCDDARTWLSSALASHRTCLDG 120

Query: 121 LEEVGAAAAVDGNNLTVLLTGALHLYDKIAAVEQRNGKHLLPT------GINLAKWDTAT 180
           LE  G A A    N+TV L+ AL LY K    +    K + PT       + LA+W   T
Sbjct: 121 LEGKGMAEAPMARNVTVWLSEALALYAKYKEPDTDAEKEVQPTLKPSQNEVMLAEWSPKT 180

Query: 181 SKADYVVAKDGSGTHTTINGAVAALARTGRRRRGERIVIYVKAGVYKENVEIGIQLNNVM 240
           SKAD VVAKDGSG H TIN AVAAL R   +R   R+V+YVK+G+Y E VEIG  LNNVM
Sbjct: 181 SKADIVVAKDGSGNHMTINEAVAALTRMVHKRT-RRVVVYVKSGIYNEKVEIGKNLNNVM 240

Query: 241 VVGDGADKTIVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPEKQQAVALRLN 300
            VGDG DKTI+T  RNV DGATT +SATFGVSGDGFWA+DITFEN AGP K QAVA+R++
Sbjct: 241 FVGDGVDKTIITADRNVHDGATTPSSATFGVSGDGFWAKDITFENRAGPHKHQAVAMRVS 300

Query: 301 SDLAVVYRCVIKGYQDTLFLHSLRQFYRDCQIYGTVDFIFGNSAAVLQNCEIFVRRPIRH 360
           SDL+V YRC  KGYQDTL++HS RQF+RDC +YGT+DFIFGN+A V QNC+I+VR+P+  
Sbjct: 301 SDLSVFYRCSFKGYQDTLYVHSNRQFFRDCHVYGTIDFIFGNAAVVFQNCDIYVRKPMNR 360

Query: 361 QANMITAQGRDDPAEATGISILNSRVRPTPDFAHVKAQYRTYLGRPWKRFSRTVVMATDL 420
           Q+NMITAQGRD P E TGIS+  SRV  +P+F  VK  ++++LGRPWKR+SRTV + TDL
Sbjct: 361 QSNMITAQGRDIPEEPTGISVQASRVLSSPEFTTVKGSFKSFLGRPWKRYSRTVFLETDL 420

Query: 421 DGLVDPKGWGEWRGDFAISTLFYAEFKNRGSGSSTRFRVNWPGFHVLRSSEEARPFTVAE 480
           DGL+DP+GW EW G++ +STL+Y E+ N G G+ST+ RV WPGFHVL  +E+A PFTV+ 
Sbjct: 421 DGLIDPRGWTEWSGNYGLSTLYYGEYNNSGGGASTKERVKWPGFHVLNGTEDAMPFTVSR 480

BLAST of Lsi05G019430 vs. TAIR10
Match: AT3G60730.1 (AT3G60730.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 518.8 bits (1335), Expect = 3.4e-147
Identity = 267/510 (52.35%), Postives = 349/510 (68.43%), Query Frame = 1

Query: 9   LLLFLPLAVVASSNSSLPELDPVRLAQAGVREARIGIANLK----RQRFGRRNLQAALAD 68
           ++ FL +A V ++ S+  ELD + +A+  V EA+    ++              +  L++
Sbjct: 13  IMFFLAIAAVITA-SNTAELDVLEMARTAVVEAKTSFGSMAVTEATSEVAGSYYKLGLSE 72

Query: 69  CATLYEEADWRLAGMLVG-ENYTAEDARTWVSAAVANHRSCLDGL-EEVGAAAAVDGNNL 128
           C  LY+E++ RL+ ++V  EN+T ED RTW+S  +ANH +CLDGL ++      +  +N+
Sbjct: 73  CEKLYDESEARLSKLVVDHENFTVEDVRTWLSGVLANHHTCLDGLIQQRQGHKPLVHSNV 132

Query: 129 TVLLTGALHLYDKI---------AAVEQRNG----KHLLPTGINLA-------------- 188
           T +L  AL  Y K              Q +G    KH  PT  N                
Sbjct: 133 TFVLHEALAFYKKSRGHMKKRLHGPARQGHGPTRPKH-RPTRPNHGPGRSHHGPSRPNQN 192

Query: 189 -----KWDTATSKADYVVAKDGSGTHTTINGAVAALARTGRRRRGERIVIYVKAGVYKEN 248
                 W+  +S+AD+VVA+DGS TH TIN A+AA++R G+ R   R++IY+KAGVY E 
Sbjct: 193 GGMLVSWNPTSSRADFVVARDGSATHRTINQALAAVSRMGKSRL-NRVIIYIKAGVYNEK 252

Query: 249 VEIGIQLNNVMVVGDGADKTIVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGP 308
           +EI   + N+M+VGDG D+TIVT +RNVPDG+TTY SATFGVSGDGFWARDITFENTAGP
Sbjct: 253 IEIDRHMKNIMLVGDGMDRTIVTNNRNVPDGSTTYGSATFGVSGDGFWARDITFENTAGP 312

Query: 309 EKQQAVALRLNSDLAVVYRCVIKGYQDTLFLHSLRQFYRDCQIYGTVDFIFGNSAAVLQN 368
            K QAVALR++SDL++ YRC  KGYQDTLF HSLRQFYRDC IYGT+DFIFG++AAV QN
Sbjct: 313 HKHQAVALRVSSDLSLFYRCSFKGYQDTLFTHSLRQFYRDCHIYGTIDFIFGDAAAVFQN 372

Query: 369 CEIFVRRPIRHQANMITAQGRDDPAEATGISILNSRVRPTPDFAHVKAQYRTYLGRPWKR 428
           C+IFVRRP+ HQ NMITAQGRDDP   +GISI +SR+R  P+F  VK ++++YLGRPWK+
Sbjct: 373 CDIFVRRPMDHQGNMITAQGRDDPHTNSGISIQHSRIRAAPEFEAVKGRFKSYLGRPWKK 432

Query: 429 FSRTVVMATDLDGLVDPKGWGEWRGDFAISTLFYAEFKNRGSGSSTRFRVNWPGFHVLRS 481
           +SRTV + TD+D L+DP+GW EW G +A+STL+Y EF N G+G+ T  RVNWPGFHVLR 
Sbjct: 433 YSRTVFLKTDIDELIDPRGWREWSGSYALSTLYYGEFMNTGAGAGTGRRVNWPGFHVLRG 492

BLAST of Lsi05G019430 vs. TAIR10
Match: AT5G51490.1 (AT5G51490.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 388.3 bits (996), Expect = 7.0e-108
Identity = 208/441 (47.17%), Postives = 275/441 (62.36%), Query Frame = 1

Query: 59  QAALADCATLYEEADWRLAGMLVG--------ENYTAEDARTWVSAAVANHRSCLDGLEE 118
           QA LADC  LY +   +L   L G        ++ T  DA+TW+S A+ N  +C  G  +
Sbjct: 98  QAVLADCIDLYGDTIMQLNRTLHGVSPKAGAAKSCTDFDAQTWLSTALTNTETCRRGSSD 157

Query: 119 VGAA----AAVDGNNLTVLLTGALHLYDKIAAVEQRNGKHLLPTGIN--LAKWDTA---T 178
           +         V    ++ L++  L +   +     +        G    L++ D      
Sbjct: 158 LNVTDFITPIVSNTKISHLISNCLAVNGALLTAGNKGNTTANQKGFPTWLSRKDKRLLRA 217

Query: 179 SKADYVVAKDGSGTHTTINGAVAALARTGRRRRGERIVIYVKAGVYKENVEIGIQLNNVM 238
            +A+ VVAKDGSG   T+  A+    R  R+    R VIYVK G+Y+EN+ + +  +++M
Sbjct: 218 VRANLVVAKDGSGHFNTVQAAIDVAGR--RKVTSGRFVIYVKRGIYQENINVRLNNDDIM 277

Query: 239 VVGDGADKTIVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPEKQQAVALRLN 298
           +VGDG   TI+TG R+V  G TTYNSAT G+ G  F A+ ITF NTAGP K QAVALR +
Sbjct: 278 LVGDGMRSTIITGGRSVQGGYTTYNSATAGIEGLHFIAKGITFRNTAGPAKGQAVALRSS 337

Query: 299 SDLAVVYRCVIKGYQDTLFLHSLRQFYRDCQIYGTVDFIFGNSAAVLQNCEIFVRRPIRH 358
           SDL++ Y+C I+GYQDTL +HS RQFYR+C IYGTVDFIFGN+AAV QNC I  RRP++ 
Sbjct: 338 SDLSIFYKCSIEGYQDTLMVHSQRQFYRECYIYGTVDFIFGNAAAVFQNCLILPRRPLKG 397

Query: 359 QANMITAQGRDDPAEATGISILNSRVRPTPDFAHVKAQYRTYLGRPWKRFSRTVVMATDL 418
           QAN+ITAQGR DP + TGISI NSR+ P PD   V    +TY+GRPW +FSRTVV+ T L
Sbjct: 398 QANVITAQGRADPFQNTGISIHNSRILPAPDLKPVVGTVKTYMGRPWMKFSRTVVLQTYL 457

Query: 419 DGLVDPKGWGEW--RGDFAISTLFYAEFKNRGSGSSTRFRVNWPGFHVLRSSEEARPFTV 478
           D +V P GW  W     F + TLFYAE+KN G  SSTR+RV+W GFHVL  + +A  FTV
Sbjct: 458 DNVVSPVGWSPWIEGSVFGLDTLFYAEYKNTGPASSTRWRVSWKGFHVLGRASDASAFTV 517

Query: 479 AEFLHGGAWIPATGVPFEAGV 481
            +F+ G AW+P TG+PF +G+
Sbjct: 518 GKFIAGTAWLPRTGIPFTSGL 536

BLAST of Lsi05G019430 vs. TAIR10
Match: AT2G45220.1 (AT2G45220.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 387.1 bits (993), Expect = 1.6e-107
Identity = 218/429 (50.82%), Postives = 274/429 (63.87%), Query Frame = 1

Query: 59  QAALADCATLYEEADWRL-AGMLVGENYTAEDARTWVSAAVANHRSCLDGLEEVGAAAAV 118
           +AA  DC  LY+    ++   M      +  DA+TW+S A+ N  +C  G  E+G    V
Sbjct: 94  KAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQTWLSTALTNLDTCRAGFLELGVTDIV 153

Query: 119 ---DGNNLTVLLTGALHLYDKIAAVEQRNGKHLLPTGINLAK---WDTATSKADYVVAKD 178
                NN++ LL   L + +K+        K   P+ +         ++T K + VVAKD
Sbjct: 154 LPLMSNNVSNLLCNTLAI-NKVPFNYTPPEKDGFPSWVKPGDRKLLQSSTPKDNAVVAKD 213

Query: 179 GSGTHTTINGAVAALARTGRRRRGERIVIYVKAGVYKENVEIGIQLNNVMVVGDGADKTI 238
           GSG   TI  A+ A + +GR       VIYVK GVY EN+EI  +  NVM+ GDG  KTI
Sbjct: 214 GSGNFKTIKEAIDAASGSGR------FVIYVKQGVYSENLEI--RKKNVMLRGDGIGKTI 273

Query: 239 VTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPEKQQAVALRLNSDLAVVYRCV 298
           +TGS++V  G TT+NSAT    GDGF AR ITF NTAG   +QAVALR  SDL+V Y+C 
Sbjct: 274 ITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNTAGASNEQAVALRSGSDLSVFYQCS 333

Query: 299 IKGYQDTLFLHSLRQFYRDCQIYGTVDFIFGNSAAVLQNCEIFVRRPIRHQANMITAQGR 358
            + YQDTL++HS RQFYRDC +YGTVDFIFGN+AAVLQNC IF RRP R + N ITAQGR
Sbjct: 334 FEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCNIFARRP-RSKTNTITAQGR 393

Query: 359 DDPAEATGISILNSRVRPTPDFAHVKAQYRTYLGRPWKRFSRTVVMATDLDGLVDPKGWG 418
            DP + TGI I NSRV    D   V    +TYLGRPW+++SRTV M T LD L+DP+GW 
Sbjct: 394 SDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWRQYSRTVFMKTSLDSLIDPRGWL 453

Query: 419 EWRGDFAISTLFYAEFKNRGSGSSTRFRVNWPGFHVLRSSEEARPFTVAEFLHGGAWIPA 478
           EW G+FA+ TLFYAEF+N G G+ST  RV WPGF VL S+ EA  FTV  FL GG+WIP+
Sbjct: 454 EWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGSASEASKFTVGTFLAGGSWIPS 511

Query: 479 TGVPFEAGV 481
           + VPF +G+
Sbjct: 514 S-VPFTSGL 511

BLAST of Lsi05G019430 vs. TAIR10
Match: AT3G47400.1 (AT3G47400.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 370.5 bits (950), Expect = 1.5e-102
Identity = 203/446 (45.52%), Postives = 273/446 (61.21%), Query Frame = 1

Query: 59  QAALADCATLYEEADWRLAGMLVGENYTAE--------DARTWVSAAVANHRSCLDGLEE 118
           +AA +DC  L++    +L   L G N  A         DA+TW+S A  N  +C  G E+
Sbjct: 152 KAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQTNIETCRSGSED 211

Query: 119 VGAA----AAVDGNNLTVLLTGALHLYDKIAAVEQR----NGKHLLPTGIN------LAK 178
           +  +      +   NL+ L+   L +   +          N K   P+ ++      L  
Sbjct: 212 LNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEYFPSWVSRHERRLLVS 271

Query: 179 WDTATSKADYVVAKDGSGTHTTINGAVAALARTGRRRRGERIVIYVKAGVYKENVEIGIQ 238
              A S    VVA+D SG   +I  A+   AR   RR   R VIYVK GVY+EN+++G  
Sbjct: 272 ASLAKSSPHLVVAQDRSGHFRSIQAAINFAAR---RRFKSRFVIYVKKGVYRENIDVGND 331

Query: 239 LNNVMVVGDGADKTIVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPEKQQAV 298
            +N+M+VGDG  KTI+T  R+V  G TTYNSAT G  G  F A+D+TF NTAGP + QAV
Sbjct: 332 NHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAKDMTFINTAGPLRGQAV 391

Query: 299 ALRLNSDLAVVYRCVIKGYQDTLFLHSLRQFYRDCQIYGTVDFIFGNSAAVLQNCEIFVR 358
           A+R +SDL+V YR  I G+QDTL++HS RQF+R+C I GT+DFIFGN+A V QNC I VR
Sbjct: 392 AVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGTIDFIFGNAAVVFQNCMILVR 451

Query: 359 RPIRHQANMITAQGRDDPAEATGISILNSRVRPTPDFAHVKAQYRTYLGRPWKRFSRTVV 418
           RP+  QAN+ITAQGR DP + TGI+I +SR+    D   V   Y+TYLGRPW+ +SR  +
Sbjct: 452 RPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVIRAYKTYLGRPWQAYSRVTI 511

Query: 419 MATDLDGLVDPKGWGEW-RG-DFAISTLFYAEFKNRGSGSSTRFRVNWPGFHVLRSSEEA 478
           M T +D  + P GW  W RG +FA++T+FY E+KN G GSSTR+RV W GFH + S+  A
Sbjct: 512 MKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSSTRWRVRWKGFHAITSTAVA 571

Query: 479 RPFTVAEFLHGGAWIPATGVPFEAGV 481
             FTV   + GG+W+PATGVPF++G+
Sbjct: 572 SRFTVGSLIAGGSWLPATGVPFKSGL 594

BLAST of Lsi05G019430 vs. TAIR10
Match: AT1G53830.1 (AT1G53830.1 pectin methylesterase 2)

HSP 1 Score: 369.8 bits (948), Expect = 2.6e-102
Identity = 196/424 (46.23%), Postives = 257/424 (60.61%), Query Frame = 1

Query: 87  AEDARTWVSAAVANHRSCLDG--------------------LEEVGAAAAVDGNNLTVLL 146
           A+D +T +S+A+ N  +CLDG                    +E + + A     N+T   
Sbjct: 164 ADDLKTLISSAITNQGTCLDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETD 223

Query: 147 TGALHLYDKIAAVEQRNGKHLLPTGINL-----AKW---------DTATSKADYVVAKDG 206
                L DK +     N + L     +L      KW           +T KAD  VA DG
Sbjct: 224 IANFELRDKSSTFTNNNNRKLKEVTGDLDSDGWPKWLSVGDRRLLQGSTIKADATVADDG 283

Query: 207 SGTHTTINGAVAALARTGRRRRGERIVIYVKAGVYKENVEIGIQLNNVMVVGDGADKTIV 266
           SG  TT+  AVAA       +  +R VI++KAGVY+ENVE+  +  N+M +GDG  KTI+
Sbjct: 284 SGDFTTVAAAVAAAPE----KSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTII 343

Query: 267 TGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPEKQQAVALRLNSDLAVVYRCVI 326
           TGSRNV DG+TT++SAT    G+ F ARDITF+NTAGP K QAVALR+ SD +  Y+C +
Sbjct: 344 TGSRNVVDGSTTFHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDM 403

Query: 327 KGYQDTLFLHSLRQFYRDCQIYGTVDFIFGNSAAVLQNCEIFVRRPIRHQANMITAQGRD 386
             YQDTL++HS RQF+  C I GTVDFIFGN+AAVLQ+C+I  RRP   Q NM+TAQGR 
Sbjct: 404 FAYQDTLYVHSNRQFFVKCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRS 463

Query: 387 DPAEATGISILNSRVRPTPDFAHVKAQYRTYLGRPWKRFSRTVVMATDLDGLVDPKGWGE 446
           DP + TGI I N R+  T D   VK  + TYLGRPWK +SRTV+M +D+  ++ P+GW E
Sbjct: 464 DPNQNTGIVIQNCRIGGTSDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHE 523

Query: 447 WRGDFAISTLFYAEFKNRGSGSSTRFRVNWPGFHVLRSSEEARPFTVAEFLHGGAWIPAT 477
           W G FA+ TL Y E+ NRG G+ T  RV W G+ V+ S  EA+PFT  +F+ GG W+ +T
Sbjct: 524 WSGSFALDTLTYREYLNRGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLAST 583

BLAST of Lsi05G019430 vs. NCBI nr
Match: gi|700201482|gb|KGN56615.1| (hypothetical protein Csa_3G126790 [Cucumis sativus])

HSP 1 Score: 810.8 bits (2093), Expect = 1.2e-231
Identity = 413/489 (84.46%), Postives = 435/489 (88.96%), Query Frame = 1

Query: 1   MPFSFATFLLL-FLPLAVVASSNSSLPELDPVRLAQAGVREARIGIANLKRQRFGRRNLQ 60
           MPF F TFLLL FLP   VASSN SLP LD VRLAQ GVREA   I NLKR++FG  NLQ
Sbjct: 1   MPFPFTTFLLLLFLPF--VASSNFSLPALDSVRLAQTGVREAITEIGNLKRRQFGDWNLQ 60

Query: 61  AALADCATLYEEADWRLAGMLVGEN-YTAEDARTWVSAAVANHRSCLDGLEEVGAAAAVD 120
           AAL DCATLYEEA+WRLAGMLVGEN Y AED R WVSAA+ANHRSCLDGLEEV   AAVD
Sbjct: 61  AALRDCATLYEEAEWRLAGMLVGENKYRAEDGRMWVSAAMANHRSCLDGLEEVHDVAAVD 120

Query: 121 GNNLTVLLTGALHLYDKIAAVEQRNGKHLLPT-------GINLAKWDTATSKADYVVAKD 180
           GNNLTV+LTGALHLYDKIAAVE+RNG+  L         G NLA W+ ATSKA+YVVAKD
Sbjct: 121 GNNLTVMLTGALHLYDKIAAVEKRNGRKRLGKRKWRENRGTNLATWNPATSKANYVVAKD 180

Query: 181 GSGTHTTINGAVAALARTGRRRRGERIVIYVKAGVYKENVEIGIQLNNVMVVGDGADKTI 240
           GSGTH TIN AVAALAR+GR RRG RIVIYVKAGVY+ENVEIGIQL NVM+VGDG DKTI
Sbjct: 181 GSGTHRTINRAVAALARSGRTRRGGRIVIYVKAGVYRENVEIGIQLKNVMLVGDGIDKTI 240

Query: 241 VTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPEKQQAVALRLNSDLAVVYRCV 300
           VTGSRNVPDGATTYNSATFGVSGDGFW RDITFENTAGP KQQAVALRLNSDLAVVYRC 
Sbjct: 241 VTGSRNVPDGATTYNSATFGVSGDGFWVRDITFENTAGPGKQQAVALRLNSDLAVVYRCA 300

Query: 301 IKGYQDTLFLHSLRQFYRDCQIYGTVDFIFGNSAAVLQNCEIFVRRPIRHQANMITAQGR 360
           IKGYQDTLFLHSLRQFYRDC+IYGT+DFIFGNSAAVLQNCEIFVR+PI HQANMITAQGR
Sbjct: 301 IKGYQDTLFLHSLRQFYRDCKIYGTIDFIFGNSAAVLQNCEIFVRQPILHQANMITAQGR 360

Query: 361 DDPAEATGISILNSRVRPTPDFAHVKAQYRTYLGRPWKRFSRTVVMATDLDGLVDPKGWG 420
           DDPAEATG SILNSRV+P P+F+ VKAQYRTYLGRPWKRFSRTVV+ATDLDGL+DPKGWG
Sbjct: 361 DDPAEATGFSILNSRVQPAPEFSPVKAQYRTYLGRPWKRFSRTVVIATDLDGLIDPKGWG 420

Query: 421 EWRGDFAISTLFYAEFKNRGSGSSTRFRVNWPGFHVLRSSEEARPFTVAEFLHGGAWIPA 480
           EW GDFAISTLFYAEFKNRG GSSTRFRVNWPGFH+LRS+EEARPFTVAEFLHGG WIPA
Sbjct: 421 EWTGDFAISTLFYAEFKNRGDGSSTRFRVNWPGFHILRSAEEARPFTVAEFLHGGDWIPA 480

BLAST of Lsi05G019430 vs. NCBI nr
Match: gi|659075523|ref|XP_008438191.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 36 isoform X1 [Cucumis melo])

HSP 1 Score: 798.5 bits (2061), Expect = 6.4e-228
Identity = 414/491 (84.32%), Postives = 434/491 (88.39%), Query Frame = 1

Query: 1   MPFSFATFLLLFLPLAVVASSNSSLPELDPVRLAQAGVREARIGIANLKRQRFGRRN-LQ 60
           MPF F TFLLL L L  VASSN SLP LD VRLAQ GVREA   I NLKR+RFG  N L 
Sbjct: 1   MPFPFTTFLLL-LCLPFVASSNFSLPALDSVRLAQTGVREAVTEIGNLKRRRFGDGNHLL 60

Query: 61  AALADCATLYEEADWRLAGMLVG--ENYTAEDARTWVSAAVANHRSCLDGLEEVGA-AAA 120
           AAL DCA LYEEA+WRLAGMLVG  + Y+AED R WVSAA+ANHRSCLDGLEEV A  AA
Sbjct: 61  AALRDCAKLYEEAEWRLAGMLVGGEKYYSAEDGRMWVSAAMANHRSCLDGLEEVDADGAA 120

Query: 121 VDGNNLTVLLTGALHLYDKIAAVEQRNGKHLLPT-------GINLAKWDTATSKADYVVA 180
           V G+NLTV+LTGALHLYDKIAAVE+RNG   L         GINLA W+ ATSKADYVVA
Sbjct: 121 VGGHNLTVMLTGALHLYDKIAAVEKRNGWKRLEKRKWRENRGINLATWNPATSKADYVVA 180

Query: 181 KDGSGTHTTINGAVAALARTGRRRRGERIVIYVKAGVYKENVEIGIQLNNVMVVGDGADK 240
           KDGSGTHTTIN AVAALA TGRRR G RIVIYVKAGVY+ENVEIGIQL NVM+VGDG DK
Sbjct: 181 KDGSGTHTTINRAVAALASTGRRRGGGRIVIYVKAGVYRENVEIGIQLKNVMLVGDGIDK 240

Query: 241 TIVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPEKQQAVALRLNSDLAVVYR 300
           TIVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPEKQQAVALRLNSDLAVVYR
Sbjct: 241 TIVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPEKQQAVALRLNSDLAVVYR 300

Query: 301 CVIKGYQDTLFLHSLRQFYRDCQIYGTVDFIFGNSAAVLQNCEIFVRRPIRHQANMITAQ 360
           C IKGYQDTLFLHSLRQFYRDC+IYGT+DFIFGNSAAVLQNCEIFVR+P RHQANMITAQ
Sbjct: 301 CAIKGYQDTLFLHSLRQFYRDCKIYGTIDFIFGNSAAVLQNCEIFVRQPRRHQANMITAQ 360

Query: 361 GRDDPAEATGISILNSRVRPTPDFAHVKAQYRTYLGRPWKRFSRTVVMATDLDGLVDPKG 420
           GRDDPAEATGISILNSRV+PTPDFA VKAQYRTYLGRPWKRFSRTVVM TDLDGL+DPKG
Sbjct: 361 GRDDPAEATGISILNSRVQPTPDFAPVKAQYRTYLGRPWKRFSRTVVMGTDLDGLIDPKG 420

Query: 421 WGEWRGDFAISTLFYAEFKNRGSGSSTRFRVNWPGFHVLRSSEEARPFTVAEFLHGGAWI 480
           WGEW G+FAISTLFYAEFKNRG GSSTRFRVNWPGFHVLRS++EARPFTVAEFLHGG WI
Sbjct: 421 WGEWTGNFAISTLFYAEFKNRGDGSSTRFRVNWPGFHVLRSADEARPFTVAEFLHGGDWI 480

BLAST of Lsi05G019430 vs. NCBI nr
Match: gi|778677487|ref|XP_004134349.2| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 36 [Cucumis sativus])

HSP 1 Score: 644.0 bits (1660), Expect = 2.0e-181
Identity = 315/363 (86.78%), Postives = 333/363 (91.74%), Query Frame = 1

Query: 125 LLTGALHLYDKIAAVEQRNGKHLLPT-------GINLAKWDTATSKADYVVAKDGSGTHT 184
           +LTGALHLYDKIAAVE+RNG+  L         G NLA W+ ATSKA+YVVAKDGSGTH 
Sbjct: 1   MLTGALHLYDKIAAVEKRNGRKRLGKRKWRENRGTNLATWNPATSKANYVVAKDGSGTHR 60

Query: 185 TINGAVAALARTGRRRRGERIVIYVKAGVYKENVEIGIQLNNVMVVGDGADKTIVTGSRN 244
           TIN AVAALAR+GR RRG RIVIYVKAGVY+ENVEIGIQL NVM+VGDG DKTIVTGSRN
Sbjct: 61  TINRAVAALARSGRTRRGGRIVIYVKAGVYRENVEIGIQLKNVMLVGDGIDKTIVTGSRN 120

Query: 245 VPDGATTYNSATFGVSGDGFWARDITFENTAGPEKQQAVALRLNSDLAVVYRCVIKGYQD 304
           VPDGATTYNSATFGVSGDGFW RDITFENTAGP KQQAVALRLNSDLAVVYRC IKGYQD
Sbjct: 121 VPDGATTYNSATFGVSGDGFWVRDITFENTAGPGKQQAVALRLNSDLAVVYRCAIKGYQD 180

Query: 305 TLFLHSLRQFYRDCQIYGTVDFIFGNSAAVLQNCEIFVRRPIRHQANMITAQGRDDPAEA 364
           TLFLHSLRQFYRDC+IYGT+DFIFGNSAAVLQNCEIFVR+PI HQANMITAQGRDDPAEA
Sbjct: 181 TLFLHSLRQFYRDCKIYGTIDFIFGNSAAVLQNCEIFVRQPILHQANMITAQGRDDPAEA 240

Query: 365 TGISILNSRVRPTPDFAHVKAQYRTYLGRPWKRFSRTVVMATDLDGLVDPKGWGEWRGDF 424
           TG SILNSRV+P P+F+ VKAQYRTYLGRPWKRFSRTVV+ATDLDGL+DPKGWGEW GDF
Sbjct: 241 TGFSILNSRVQPAPEFSPVKAQYRTYLGRPWKRFSRTVVIATDLDGLIDPKGWGEWTGDF 300

Query: 425 AISTLFYAEFKNRGSGSSTRFRVNWPGFHVLRSSEEARPFTVAEFLHGGAWIPATGVPFE 481
           AISTLFYAEFKNRG GSSTRFRVNWPGFH+LRS+EEARPFTVAEFLHGG WIPATGVPFE
Sbjct: 301 AISTLFYAEFKNRGDGSSTRFRVNWPGFHILRSAEEARPFTVAEFLHGGDWIPATGVPFE 360

BLAST of Lsi05G019430 vs. NCBI nr
Match: gi|823208292|ref|XP_012437591.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 36 [Gossypium raimondii])

HSP 1 Score: 560.1 bits (1442), Expect = 3.8e-156
Identity = 285/482 (59.13%), Postives = 353/482 (73.24%), Query Frame = 1

Query: 7   TFLLLFLPLAVVASSNSSLPEL-DPVRLAQAGVREARIGIANLKRQRFGRRNLQ----AA 66
           TFLLLFL     A++ S L ++ + V++A+  V ++R  +A      +G  N        
Sbjct: 18  TFLLLFLLAMATATTGSHLEQVTEVVQMARTMVVQSR-NLAKASMSLYGLHNTMHYHGMG 77

Query: 67  LADCATLYEEADWRLAGMLVGENYTAEDARTWVSAAVANHRSCLDGL--EEVGAAAAVDG 126
           LADC  LY+E++ RL+ +L    YT +DARTW+S  +ANHRSC +GL  ++ G      G
Sbjct: 78  LADCIKLYDESESRLSVLLSSRGYTVDDARTWLSGVLANHRSCSEGLGQKDFGIQEHGQG 137

Query: 127 NNLTVLLTGALHLYD---KIAAVEQRNGKHLLPTGINLAKWDTATSKADYVVAKDGSGTH 186
            NLT L++ AL LY    K A VEQ         G  L  W+  TSKAD+VV+KDGSGTH
Sbjct: 138 RNLTSLVSAALALYTMHGKEAVVEQELMHRPRLGGKLLTSWNPTTSKADFVVSKDGSGTH 197

Query: 187 TTINGAVAALARTGRRRRGERIVIYVKAGVYKENVEIGIQLNNVMVVGDGADKTIVTGSR 246
            TIN AVAA+AR G  RR  R++IYVKAGVY E V+I   L N+M+VGDG DKTIVTG+R
Sbjct: 198 KTINDAVAAVARIGNNRR-PRVIIYVKAGVYNEKVDIDKNLKNIMLVGDGMDKTIVTGNR 257

Query: 247 NVPDGATTYNSATFGVSGDGFWARDITFENTAGPEKQQAVALRLNSDLAVVYRCVIKGYQ 306
           NVPDG+TTY SATFGVSGDGFWARD+TF NTAGP K QAVALR++SD +V YRC IKGYQ
Sbjct: 258 NVPDGSTTYGSATFGVSGDGFWARDVTFVNTAGPHKHQAVALRVSSDHSVFYRCSIKGYQ 317

Query: 307 DTLFLHSLRQFYRDCQIYGTVDFIFGNSAAVLQNCEIFVRRPIRHQANMITAQGRDDPAE 366
           D+LFLHSLRQFYRDC I+GT+D+IFG+++AVLQNC+IFV+RP+ HQ+NM+TAQGRDDP E
Sbjct: 318 DSLFLHSLRQFYRDCHIHGTIDYIFGDASAVLQNCDIFVKRPMDHQSNMVTAQGRDDPNE 377

Query: 367 ATGISILNSRVRPTPDFAHVKAQYRTYLGRPWKRFSRTVVMATDLDGLVDPKGWGEWRGD 426
            TGISI+ SRVRP PDF  VK  +++YLGRPWK++SRTV M TDLDGL+DPKGW EW G 
Sbjct: 378 NTGISIMESRVRPAPDFDSVKHLFKSYLGRPWKKYSRTVFMKTDLDGLIDPKGWNEWSGS 437

Query: 427 FAISTLFYAEFKNRGSGSSTRFRVNWPGFHVLRSSEEARPFTVAEFLHGGAWIPATGVPF 479
           FA+STL+YAE++N G+G+S   RV WPGFHV   +EEA PFTV  F+ G  WIP TGVPF
Sbjct: 438 FALSTLYYAEYRNTGNGASIGNRVKWPGFHVFNRAEEANPFTVNRFIQGELWIPETGVPF 497

BLAST of Lsi05G019430 vs. NCBI nr
Match: gi|1000981996|ref|XP_002511680.2| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 36 [Ricinus communis])

HSP 1 Score: 545.4 bits (1404), Expect = 9.8e-152
Identity = 283/486 (58.23%), Postives = 347/486 (71.40%), Query Frame = 1

Query: 6   ATFLLLFLPLAVVASSNSSLPELDPVRLAQAGVREAR----IGIANLKRQRFGRRNLQAA 65
           +T ++LF  LA+  SS   L EL+ ++ AQ    + R      +     Q F   N    
Sbjct: 2   STIVILFFLLAMATSSTFCLQELETIQTAQTQTLQVRKWLQSSLGGGLNQGFSY-NFGVP 61

Query: 66  LADCATLYEEADWRLAGMLVGE-NYTAEDARTWVSAAVANHRSCLDGLEEVGAAAAVD-- 125
           L+DC  LY E+++RL+ +L  E NYT  DARTW+S  +ANH++CLDGL E G        
Sbjct: 62  LSDCIKLYSESEFRLSQLLASEKNYTHHDARTWLSGVLANHKTCLDGLSEKGFLENDHEM 121

Query: 126 GNNLTVLLTGALHLYDK----IAAVEQRNGKHLLPTGINLAKWDTATSKADYVVAKDGSG 185
            +NLT  L+ +L LY +    I     R   H    GI L  W+  TS+AD+VVA+DGSG
Sbjct: 122 AHNLTFSLSKSLALYSRGRRTINRGVPRRPIHNYNGGI-LTSWNPTTSQADFVVARDGSG 181

Query: 186 THTTINGAVAALARTGRRRRGERIVIYVKAGVYKENVEIGIQLNNVMVVGDGADKTIVTG 245
           TH TIN A+AAL+R G RR  +R++IYVKAGVY E VEI   + +VM VGDG DKTI+TG
Sbjct: 182 THRTINDALAALSRLGTRRT-QRVIIYVKAGVYNEKVEIDHHIKDVMFVGDGIDKTIITG 241

Query: 246 SRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPEKQQAVALRLNSDLAVVYRCVIKG 305
           SRNVPDG++T++SATFGVSGDGFWARDITFENTAGP K QAVALR++SDL+V YRC  K 
Sbjct: 242 SRNVPDGSSTFSSATFGVSGDGFWARDITFENTAGPHKHQAVALRVSSDLSVFYRCSFKA 301

Query: 306 YQDTLFLHSLRQFYRDCQIYGTVDFIFGNSAAVLQNCEIFVRRPIRHQANMITAQGRDDP 365
           YQDTLF+ SLRQFYRDC IYGT+DFIFG++  V QNC+IFVRRP+ HQAN ITAQGRDDP
Sbjct: 302 YQDTLFVLSLRQFYRDCHIYGTIDFIFGDAPVVFQNCDIFVRRPMDHQANFITAQGRDDP 361

Query: 366 AEATGISILNSRVRPTPDFAHVKAQYRTYLGRPWKRFSRTVVMATDLDGLVDPKGWGEWR 425
            E TGISI  SRVR +PDF   K + R++LGRPWK++SRTV M TDLDGL+DP GWG W 
Sbjct: 362 NENTGISIQRSRVRASPDFMVAKNKIRSFLGRPWKKYSRTVFMQTDLDGLIDPMGWGAWH 421

Query: 426 GDFAISTLFYAEFKNRGSGSSTRFRVNWPGFHVLRSSEEARPFTVAEFLHGGAWIPATGV 481
           GDFA+STLFYAE+ N G+G+ST  RV WPGFHVL S ++A PFTV  F+ G +WIPATGV
Sbjct: 422 GDFALSTLFYAEYMNTGNGASTSRRVKWPGFHVLSSPQQASPFTVTRFIQGESWIPATGV 481

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PME36_ARATH6.1e-14652.35Probable pectinesterase/pectinesterase inhibitor 36 OS=Arabidopsis thaliana GN=P... [more]
PME2_CITSI1.6e-10950.70Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1[more]
PME59_ARATH1.2e-10647.17Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana GN=P... [more]
PME17_ARATH2.8e-10650.82Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana GN=P... [more]
PME1_FICPW1.4e-10545.37Pectinesterase/pectinesterase inhibitor OS=Ficus pumila var. awkeotsang PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A0A0L3U7_CUCSA8.7e-23284.46Pectinesterase OS=Cucumis sativus GN=Csa_3G126790 PE=4 SV=1[more]
A0A0D2PSW3_GOSRA2.7e-15659.13Pectinesterase OS=Gossypium raimondii GN=B456_008G113200 PE=4 SV=1[more]
A0A078C450_BRANA1.7e-15053.91Pectinesterase OS=Brassica napus GN=BnaC08g30880D PE=4 SV=1[more]
B9RD91_RICCO8.3e-15058.53Pectinesterase OS=Ricinus communis GN=RCOM_1611150 PE=4 SV=1[more]
D7U7W0_VITVI6.0e-14854.91Pectinesterase OS=Vitis vinifera GN=VIT_15s0048g00500 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G60730.13.4e-14752.35 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT5G51490.17.0e-10847.17 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT2G45220.11.6e-10750.82 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT3G47400.11.5e-10245.52 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT1G53830.12.6e-10246.23 pectin methylesterase 2[more]
Match NameE-valueIdentityDescription
gi|700201482|gb|KGN56615.1|1.2e-23184.46hypothetical protein Csa_3G126790 [Cucumis sativus][more]
gi|659075523|ref|XP_008438191.1|6.4e-22884.32PREDICTED: probable pectinesterase/pectinesterase inhibitor 36 isoform X1 [Cucum... [more]
gi|778677487|ref|XP_004134349.2|2.0e-18186.78PREDICTED: probable pectinesterase/pectinesterase inhibitor 36 [Cucumis sativus][more]
gi|823208292|ref|XP_012437591.1|3.8e-15659.13PREDICTED: probable pectinesterase/pectinesterase inhibitor 36 [Gossypium raimon... [more]
gi|1000981996|ref|XP_002511680.2|9.8e-15258.23PREDICTED: probable pectinesterase/pectinesterase inhibitor 36 [Ricinus communis... [more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004857enzyme inhibitor activity
GO:0030599pectinesterase activity
Vocabulary: Biological Process
TermDefinition
GO:0042545cell wall modification
Vocabulary: Cellular Component
TermDefinition
GO:0005618cell wall
Vocabulary: INTERPRO
TermDefinition
IPR012334Pectin_lyas_fold
IPR011050Pectin_lyase_fold/virulence
IPR006501Pectinesterase_inhib_dom
IPR000070Pectinesterase_cat
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043481 anthocyanin accumulation in tissues in response to UV light
biological_process GO:0010048 vernalization response
biological_process GO:0016485 protein processing
biological_process GO:0070084 protein initiator methionine removal
biological_process GO:0031365 N-terminal protein amino acid modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0048440 carpel development
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0005982 starch metabolic process
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005618 cell wall
cellular_component GO:0022626 cytosolic ribosome
cellular_component GO:0005576 extracellular region
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0070006 metalloaminopeptidase activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0008270 zinc ion binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G019430.1Lsi05G019430.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 165..465
score: 1.4E
IPR006501Pectinesterase inhibitor domainGENE3DG3DSA:1.20.140.40coord: 32..114
score: 5.
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 31..123
score: 6.
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 13..132
score: 0.
IPR006501Pectinesterase inhibitor domainunknownSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 31..114
score: 7.4
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 160..478
score: 3.77E
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 161..478
score: 1.2E
NoneNo IPR availablePANTHERPTHR31707FAMILY NOT NAMEDcoord: 31..480
score: 2.8E
NoneNo IPR availablePANTHERPTHR31707:SF35PECTINESTERASE/PECTINESTERASE INHIBITOR 36-RELATEDcoord: 31..480
score: 2.8E

The following gene(s) are paralogous to this gene:

None