Lsi05G017030 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi05G017030
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Enoyl-CoA hydratase/isomerase family protein
Location	chr05 : 24566626 .. 24567360 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTATCATTTGAAAATGTGCACAATTGAGAAGCGAGGCAATTTCTTTATCCTAACTCTGACCGGCAACGACGACCACCGCCTCGGCCCATCCTTAATCAACTCCATCCTCAAAGCTCTTTCACAAGTCAAAGCACAAGCCTCCGCCGGTTCAGTTCTCATCACAACTTCCCATGGCAAATTCTTCTCAAACGGCTTTGATCTCCCCTGGGCCCTTGCCGCCGGCTCTGTTTCTGCTGCCCGTGACCGCCTAAAGCACATAATCGAGATCTTCAAGCCCCTCATAGCCGAACTCCTTTCCCTTCCTATGCCTACCATTGCTGCCATTACAGGACACGCCGCTGCCGCCGGTTTCACATTGGCGCTGAGCCACGACTATTTGATTATGAGGAGCGATCGAGGCGTGCTGTACATGCGTGAGTTGGACCTGGGTCTAACTCTACCGGATTACTTTATGGCTCTGTTTCAGTCCAAGACAGGATCCAGTTTGGCTATGCGCGACCTGATCCTGAGGGGGATGAAAGTGAGAGGTGAAGCGGCAGTGAGAATGGGCATCGTGGAATCGGCGCACCACGGCCAGGACGGCGTCGTGAGCGCTGCAGTGCGGCTTGGGGAGCAGCTGGCGAGGAGAAAATGGGATGGGGAAGTTTATGCTGAAATCAGGAAGAGTTTGTATCCTGAGGTCTCTCAGGTGCTTGGGTTACTGGGGAAAGCCATATCCATTTCTAAACTATGA

mRNA sequence

ATGTATCATTTGAAAATGTGCACAATTGAGAAGCGAGGCAATTTCTTTATCCTAACTCTGACCGGCAACGACGACCACCGCCTCGGCCCATCCTTAATCAACTCCATCCTCAAAGCTCTTTCACAAGTCAAAGCACAAGCCTCCGCCGGTTCAGTTCTCATCACAACTTCCCATGGCAAATTCTTCTCAAACGGCTTTGATCTCCCCTGGGCCCTTGCCGCCGGCTCTGTTTCTGCTGCCCGTGACCGCCTAAAGCACATAATCGAGATCTTCAAGCCCCTCATAGCCGAACTCCTTTCCCTTCCTATGCCTACCATTGCTGCCATTACAGGACACGCCGCTGCCGCCGGTTTCACATTGGCGCTGAGCCACGACTATTTGATTATGAGGAGCGATCGAGGCGTGCTGTACATGCGTGAGTTGGACCTGGGTCTAACTCTACCGGATTACTTTATGGCTCTGTTTCAGTCCAAGACAGGATCCAGTTTGGCTATGCGCGACCTGATCCTGAGGGGGATGAAAGTGAGAGGTGAAGCGGCAGTGAGAATGGGCATCGTGGAATCGGCGCACCACGGCCAGGACGGCGTCGTGAGCGCTGCAGTGCGGCTTGGGGAGCAGCTGGCGAGGAGAAAATGGGATGGGGAAGTTTATGCTGAAATCAGGAAGAGTTTGTATCCTGAGGTCTCTCAGGTGCTTGGGTTACTGGGGAAAGCCATATCCATTTCTAAACTATGA

Coding sequence (CDS)

ATGTATCATTTGAAAATGTGCACAATTGAGAAGCGAGGCAATTTCTTTATCCTAACTCTGACCGGCAACGACGACCACCGCCTCGGCCCATCCTTAATCAACTCCATCCTCAAAGCTCTTTCACAAGTCAAAGCACAAGCCTCCGCCGGTTCAGTTCTCATCACAACTTCCCATGGCAAATTCTTCTCAAACGGCTTTGATCTCCCCTGGGCCCTTGCCGCCGGCTCTGTTTCTGCTGCCCGTGACCGCCTAAAGCACATAATCGAGATCTTCAAGCCCCTCATAGCCGAACTCCTTTCCCTTCCTATGCCTACCATTGCTGCCATTACAGGACACGCCGCTGCCGCCGGTTTCACATTGGCGCTGAGCCACGACTATTTGATTATGAGGAGCGATCGAGGCGTGCTGTACATGCGTGAGTTGGACCTGGGTCTAACTCTACCGGATTACTTTATGGCTCTGTTTCAGTCCAAGACAGGATCCAGTTTGGCTATGCGCGACCTGATCCTGAGGGGGATGAAAGTGAGAGGTGAAGCGGCAGTGAGAATGGGCATCGTGGAATCGGCGCACCACGGCCAGGACGGCGTCGTGAGCGCTGCAGTGCGGCTTGGGGAGCAGCTGGCGAGGAGAAAATGGGATGGGGAAGTTTATGCTGAAATCAGGAAGAGTTTGTATCCTGAGGTCTCTCAGGTGCTTGGGTTACTGGGGAAAGCCATATCCATTTCTAAACTATGA

Protein sequence

MYHLKMCTIEKRGNFFILTLTGNDDHRLGPSLINSILKALSQVKAQASAGSVLITTSHGKFFSNGFDLPWALAAGSVSAARDRLKHIIEIFKPLIAELLSLPMPTIAAITGHAAAAGFTLALSHDYLIMRSDRGVLYMRELDLGLTLPDYFMALFQSKTGSSLAMRDLILRGMKVRGEAAVRMGIVESAHHGQDGVVSAAVRLGEQLARRKWDGEVYAEIRKSLYPEVSQVLGLLGKAISISKL

Homology

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BLAST of Lsi05G017030 vs. Swiss-Prot
Match: ECI2_ARATH (Enoyl-CoA delta isomerase 2, peroxisomal OS=Arabidopsis thaliana GN=ECI2 PE=1 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 6.2e-78
Identity = 145/240 (60.42%), Postives = 187/240 (77.92%), Query Frame = 1


Query: 6   MCTIEKRGNFFILTLTGNDDHRLGPSLINSILKALSQVKAQASAGSVLITTSHGKFFSNG 65
           MCT+EKRG+ F+LTLTG+ +HR  P  I +IL  L Q K+Q++ GS+LITT++GKFFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGDGEHRFHPDTIATILSLLEQAKSQSTRGSILITTANGKFFSNG 60

Query: 66  FDLPWALAAGSVSAARDRLKHIIEIFKPLIAELLSLPMPTIAAITGHAAAAGFTLALSHD 125
           FDL WA  AGS + A +RL  ++E FKP++A LL LPMPTIAA+ GHAAAAG  LALSHD
Sbjct: 61  FDLAWAQTAGSKTGAANRLHQMVESFKPVVAALLDLPMPTIAALNGHAAAAGLILALSHD 120

Query: 126 YLIMRSDRGVLYMRELDLGLTLPDYFMALFQSKTGSSLAMRDLILRGMKVRGEAAVRMGI 185
           Y+ MR DRGVLYM E+D+GL++PDYF AL ++K G+S A R+L+L G K+RGE AV +GI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLSMPDYFSALVRAKIGTSAARRELLLSGKKIRGEEAVGLGI 180

Query: 186 VES-AHHGQDGVVSAAVRLGEQLARRKWDGEVYAEIRKSLYPEVSQVLGLLGKAISISKL 245
           V+S A+  ++GVV A+VRLGE+LA +KW GEVYA IRKSLYPE+  +LGL  +  +  KL
Sbjct: 181 VDSAAYDSEEGVVVASVRLGEKLAAKKWSGEVYASIRKSLYPELCGILGLETRVFATPKL 240

[Close]

BLAST of Lsi05G017030 vs. Swiss-Prot
Match: ECI3_ARATH (Enoyl-CoA delta isomerase 3 OS=Arabidopsis thaliana GN=ECI3 PE=1 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 3.5e-73
Identity = 139/240 (57.92%), Postives = 177/240 (73.75%), Query Frame = 1


Query: 6   MCTIEKRGNFFILTLTGNDDHRLGPSLINSILKALSQVKAQASAGSVLITTSHGKFFSNG 65
           MCT+EKRG+ F+LTLTG D+HR  P  I S+L  L Q K+Q++ GSVLITT HGKFFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGEDEHRFHPDTIASVLSLLEQAKSQSTKGSVLITTGHGKFFSNG 60

Query: 66  FDLPWALAAGSVSAARDRLKHIIEIFKPLIAELLSLPMPTIAAITGHAAAAGFTLALSHD 125
           FDL WA +AG    A  R+  +++ FKP++A LL LPMPTIAA+ GHAAA+G   ALSHD
Sbjct: 61  FDLAWAQSAGH--GAIKRMHQMVKSFKPVLAALLDLPMPTIAALNGHAAASGLMFALSHD 120

Query: 126 YLIMRSDRGVLYMRELDLGLTLPDYFMALFQSKTGSSLAMRDLILRGMKVRGEAAVRMGI 185
           Y+ MR DRGVLYM E+D+GL +PDYF AL  +K GS +A R+L+L G K++GE AV +GI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLPVPDYFSALVVAKVGSGIARRELLLSGKKLKGEEAVALGI 180

Query: 186 VES-AHHGQDGVVSAAVRLGEQLARRKWDGEVYAEIRKSLYPEVSQVLGLLGKAISISKL 245
           V+S AH   +GVV A V LGE LA +KW+GEVYA IRKSLYPE+ +++ L    ++   L
Sbjct: 181 VDSAAHDSAEGVVEATVSLGESLAAKKWNGEVYATIRKSLYPELCRMVDLTANNLATHNL 238

[Close]

BLAST of Lsi05G017030 vs. Swiss-Prot
Match: ECI1_ARATH (Enoyl-CoA delta isomerase 1, peroxisomal OS=Arabidopsis thaliana GN=ECI1 PE=1 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 2.8e-54
Identity = 112/224 (50.00%), Postives = 157/224 (70.09%), Query Frame = 1


Query: 6   MCTIEKRGNFFILTLTGNDDHRLGPSLINSILKALSQVKAQAS-AGSVLITTSHGKFFSN 65
           MC++EKR   FIL LTG+ +HRL P+L++S+   ++Q+++  S + SVLITTS GKFFSN
Sbjct: 1   MCSLEKRDRLFILKLTGDGEHRLNPTLLDSLRSTINQIRSDPSFSQSVLITTSDGKFFSN 60

Query: 66  GFDLPWALAAGSVSAARDRLKHIIEIFKPLIAELLSLPMPTIAAITGHAAAAGFTLALSH 125
           G+DL  A +  S+S   D         + L+A+L+SLPMPTIAA+TGHA+AAG  LA+SH
Sbjct: 61  GYDLALAESNPSLSVVMDAK------LRSLVADLISLPMPTIAAVTGHASAAGCILAMSH 120

Query: 126 DYLIMRSDRGVLYMRELDLGLTLPDYFMALFQSKTGSSLAMRDLILRGMKVRGEAAVRMG 185
           DY++MR DRG LYM ELD+ L +P +FMA+ + K GS  A RD++L   KV  +  V+MG
Sbjct: 121 DYVLMRRDRGFLYMSELDIELIVPAWFMAVIRGKIGSPAARRDVMLTAAKVTADVGVKMG 180

Query: 186 IVESAHHGQDGVVSAAVRLGEQLARRKWDGEVYAEIRKSLYPEV 229
           IV+SA+      V AA++LGE++ +R  DG VY ++R+SL  EV
Sbjct: 181 IVDSAYGSAAETVEAAIKLGEEIVQRGGDGHVYGKMRESLLREV 218

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BLAST of Lsi05G017030 vs. TrEMBL
Match: A0A0A0L9X4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G141770 PE=4 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 2.4e-113
Identity = 214/239 (89.54%), Postives = 226/239 (94.56%), Query Frame = 1


Query: 6   MCTIEKRGNFFILTLTGNDDHRLGPSLINSILKALSQVKAQASAGSVLITTSHGKFFSNG 65
           MCT+EKRGNFFILTLTG+DDHRL PSLINSILKALSQVKAQAS  SVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLSPSLINSILKALSQVKAQASPASVLITTSHGKFFSNG 60

Query: 66  FDLPWALAAGSVSAARDRLKHIIEIFKPLIAELLSLPMPTIAAITGHAAAAGFTLALSHD 125
           FDLPWAL+AGSVSAAR RL H+IEIFK ++AEL+SLPMPTIAAITGHAAAAGFTLALSHD
Sbjct: 61  FDLPWALSAGSVSAARHRLNHLIEIFKSVVAELISLPMPTIAAITGHAAAAGFTLALSHD 120

Query: 126 YLIMRSDRGVLYMRELDLGLTLPDYFMALFQSKTGSSLAMRDLILRGMKVRGEAAVRMGI 185
           YLIMRSDRGVLYMRELDLGLTLPDYFMALF+SK GSSLAMRDLILRGMKVRGEAAVRMGI
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKIGSSLAMRDLILRGMKVRGEAAVRMGI 180

Query: 186 VESAHHGQDGVVSAAVRLGEQLARRKWDGEVYAEIRKSLYPEVSQVLGLLGKAISISKL 245
           V+SAH G+D VV+AAVRLGEQLA R WDGEVYAEIRKSLYPEVS+VLGL GK ISISKL
Sbjct: 181 VDSAHDGEDSVVNAAVRLGEQLAGRNWDGEVYAEIRKSLYPEVSEVLGLPGKPISISKL 239

[Close]

BLAST of Lsi05G017030 vs. TrEMBL
Match: A0A0A0L8C7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G141780 PE=4 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 1.4e-97
Identity = 182/239 (76.15%), Postives = 213/239 (89.12%), Query Frame = 1


Query: 6   MCTIEKRGNFFILTLTGNDDHRLGPSLINSILKALSQVKAQASAGSVLITTSHGKFFSNG 65
           MCT+EKR N FILTLTG+DDHR  PS+I +IL+ALS+VK+QAS GSVLITTSHGKFFSNG
Sbjct: 1   MCTLEKRRNLFILTLTGHDDHRFSPSVIAAILEALSKVKSQASTGSVLITTSHGKFFSNG 60

Query: 66  FDLPWALAAGSVSAARDRLKHIIEIFKPLIAELLSLPMPTIAAITGHAAAAGFTLALSHD 125
           FDL WA AAGSVSAA +RL H+++IFKP++AELLSLPMPTIAAI+GHAAAAGF LALSHD
Sbjct: 61  FDLSWAQAAGSVSAAAERLHHMVQIFKPVVAELLSLPMPTIAAISGHAAAAGFLLALSHD 120

Query: 126 YLIMRSDRGVLYMRELDLGLTLPDYFMALFQSKTGSSLAMRDLILRGMKVRGEAAVRMGI 185
           YL+MRSDRGVLYM E+DLGL+LPDYF ALF+SK  SS   RD+++RGMKV+GEAAV+MGI
Sbjct: 121 YLLMRSDRGVLYMSEVDLGLSLPDYFAALFKSKIASSSVRRDVLMRGMKVKGEAAVKMGI 180

Query: 186 VESAHHGQDGVVSAAVRLGEQLARRKWDGEVYAEIRKSLYPEVSQVLGLLGKAISISKL 245
            +SAH G++GV+ AAVRLGEQL  RKW+GE YAEIRKSLYPE+S++LGL  KAISISKL
Sbjct: 181 ADSAHDGENGVMEAAVRLGEQLGERKWNGEPYAEIRKSLYPEISRLLGLPEKAISISKL 239

[Close]

BLAST of Lsi05G017030 vs. TrEMBL
Match: M5XMU9_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa010715mg PE=4 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 2.9e-82
Identity = 154/229 (67.25%), Postives = 188/229 (82.10%), Query Frame = 1


Query: 6   MCTIEKRGNFFILTLTGNDDHRLGPSLINSILKALSQVKAQASAGSVLITTSHGKFFSNG 65
           MCT+EKRG+ F LTLT +D+HRL  +LI+S+L ALSQ K+QA+ GSVL+TT+HGKFFSNG
Sbjct: 1   MCTLEKRGDLFFLTLTCDDEHRLSLTLIDSLLSALSQAKSQATRGSVLVTTAHGKFFSNG 60

Query: 66  FDLPWALAAGSVSAARDRLKHIIEIFKPLIAELLSLPMPTIAAITGHAAAAGFTLALSHD 125
           FDL WA AAGS SAAR RL  ++  FKP++A LLSLPMPT+AA+ GHAAAAGF LALSHD
Sbjct: 61  FDLGWAQAAGSASAARARLSQMVAAFKPVVAALLSLPMPTVAAVQGHAAAAGFLLALSHD 120

Query: 126 YLIMRSDRGVLYMRELDLGLTLPDYFMALFQSKTGSSLAMRDLILRGMKVRGEAAVRMGI 185
           Y +MR DRGVLYM E+DLGL  PDYF A F +K GS    RD++LRGMK++G+ AV++GI
Sbjct: 121 YFLMRRDRGVLYMSEVDLGLPFPDYFTAAFLAKIGSVSGRRDVMLRGMKLKGDEAVKLGI 180

Query: 186 VESAHHGQDGVVSAAVRLGEQLARRKWDGEVYAEIRKSLYPEVSQVLGL 235
           VESAH   +  V AAVRLGEQLA+RKW+G+VYAEIRKSLYPE+  V+G+
Sbjct: 181 VESAHDSAESTVEAAVRLGEQLAKRKWNGDVYAEIRKSLYPELCGVVGV 229

[Close]

BLAST of Lsi05G017030 vs. TrEMBL
Match: A0A061EMT3_THECC (Indole-3-butyric acid response 10 OS=Theobroma cacao GN=TCM_020837 PE=4 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 4.9e-82
Identity = 149/239 (62.34%), Postives = 192/239 (80.33%), Query Frame = 1


Query: 6   MCTIEKRGNFFILTLTGNDDHRLGPSLINSILKALSQVKAQASAGSVLITTSHGKFFSNG 65
           MCT+EKRGN F+LTLTG+D HRL P+LI S+L ALSQ KAQ++ G  LIT + G+FFSNG
Sbjct: 1   MCTLEKRGNLFLLTLTGDDQHRLNPTLITSLLSALSQAKAQSTRGCALITVAQGRFFSNG 60

Query: 66  FDLPWALAAGSVSAARDRLKHIIEIFKPLIAELLSLPMPTIAAITGHAAAAGFTLALSHD 125
           FDL WA +AGS   A++RL H++E FKP++A LL+LPMPT+ A+TGHA+AAGF LALSHD
Sbjct: 61  FDLAWAQSAGSKQGAQERLLHLVESFKPVVAALLNLPMPTVTAVTGHASAAGFALALSHD 120

Query: 126 YLIMRSDRGVLYMRELDLGLTLPDYFMALFQSKTGSSLAMRDLILRGMKVRGEAAVRMGI 185
           Y +MR DRGVLYM E+D+GL++PDYFMALF+ K G++ A RDL+LRG+K++G+ A + GI
Sbjct: 121 YAVMRRDRGVLYMAEVDIGLSVPDYFMALFKEKMGAASARRDLLLRGLKMKGDEAEKRGI 180

Query: 186 VESAHHGQDGVVSAAVRLGEQLARRKWDGEVYAEIRKSLYPEVSQVLGLLGKAISISKL 245
           VE+A+     V  A+VR+GE LA+RKWDGEVYAEIRK LYPE+S +LGL  K  +  +L
Sbjct: 181 VEAAYDSDGEVREASVRMGEDLAKRKWDGEVYAEIRKGLYPELSAMLGLATKVFASPRL 239

[Close]

BLAST of Lsi05G017030 vs. TrEMBL
Match: F6H8I1_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_05s0049g00180 PE=4 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 1.9e-81
Identity = 153/239 (64.02%), Postives = 194/239 (81.17%), Query Frame = 1


Query: 6   MCTIEKRGNFFILTLTGNDDHRLGPSLINSILKALSQVKAQASAGSVLITTSHGKFFSNG 65
           MCT+E+RGN F LTLTG+++HRLGPSL +SI  AL+QVK+QA+ GS LITT+ GKFFSNG
Sbjct: 1   MCTLEQRGNLFFLTLTGDNEHRLGPSLFDSIRSALAQVKSQATPGSALITTAQGKFFSNG 60

Query: 66  FDLPWALAAGSVSAARDRLKHIIEIFKPLIAELLSLPMPTIAAITGHAAAAGFTLALSHD 125
            DL WA AAGS S+ RDRL H+++ FKP++A+LLSLPMPTIAA++GHAAAAGF LA+SHD
Sbjct: 61  LDLAWAQAAGS-SSFRDRLSHLLDCFKPIVADLLSLPMPTIAAVSGHAAAAGFMLAISHD 120

Query: 126 YLIMRSDRGVLYMRELDLGLTLPDYFMALFQSKTGSSLAMRDLILRGMKVRGEAAVRMGI 185
           Y++M++DR VLYM ELD+GLT PDYFMA+ +SK     A RD++LR  KV+ E AVRMGI
Sbjct: 121 YVLMKNDRSVLYMSELDIGLTFPDYFMAMMKSKISDPAARRDVMLRAKKVKAEEAVRMGI 180

Query: 186 VESAHHGQDGVVSAAVRLGEQLARRKWDGEVYAEIRKSLYPEVSQVLGLLGKAISISKL 245
           V+SAH   +  V AAVRL EQL+ RKW+GEVYAEIRK+++PEV  +LGL  +A+   +L
Sbjct: 181 VDSAHDSAESTVEAAVRLAEQLSGRKWNGEVYAEIRKAMHPEVCGLLGLSHRAVVAPRL 238

[Close]

BLAST of Lsi05G017030 vs. TAIR10
Match: AT4G14430.1 (AT4G14430.1 indole-3-butyric acid response 10)

HSP 1 Score: 292.0 bits (746), Expect = 3.5e-79
Identity = 145/240 (60.42%), Postives = 187/240 (77.92%), Query Frame = 1


Query: 6   MCTIEKRGNFFILTLTGNDDHRLGPSLINSILKALSQVKAQASAGSVLITTSHGKFFSNG 65
           MCT+EKRG+ F+LTLTG+ +HR  P  I +IL  L Q K+Q++ GS+LITT++GKFFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGDGEHRFHPDTIATILSLLEQAKSQSTRGSILITTANGKFFSNG 60

Query: 66  FDLPWALAAGSVSAARDRLKHIIEIFKPLIAELLSLPMPTIAAITGHAAAAGFTLALSHD 125
           FDL WA  AGS + A +RL  ++E FKP++A LL LPMPTIAA+ GHAAAAG  LALSHD
Sbjct: 61  FDLAWAQTAGSKTGAANRLHQMVESFKPVVAALLDLPMPTIAALNGHAAAAGLILALSHD 120

Query: 126 YLIMRSDRGVLYMRELDLGLTLPDYFMALFQSKTGSSLAMRDLILRGMKVRGEAAVRMGI 185
           Y+ MR DRGVLYM E+D+GL++PDYF AL ++K G+S A R+L+L G K+RGE AV +GI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLSMPDYFSALVRAKIGTSAARRELLLSGKKIRGEEAVGLGI 180

Query: 186 VES-AHHGQDGVVSAAVRLGEQLARRKWDGEVYAEIRKSLYPEVSQVLGLLGKAISISKL 245
           V+S A+  ++GVV A+VRLGE+LA +KW GEVYA IRKSLYPE+  +LGL  +  +  KL
Sbjct: 181 VDSAAYDSEEGVVVASVRLGEKLAAKKWSGEVYASIRKSLYPELCGILGLETRVFATPKL 240

[Close]

BLAST of Lsi05G017030 vs. TAIR10
Match: AT4G14440.1 (AT4G14440.1 3-hydroxyacyl-CoA dehydratase 1)

HSP 1 Score: 276.2 bits (705), Expect = 2.0e-74
Identity = 139/240 (57.92%), Postives = 177/240 (73.75%), Query Frame = 1


Query: 6   MCTIEKRGNFFILTLTGNDDHRLGPSLINSILKALSQVKAQASAGSVLITTSHGKFFSNG 65
           MCT+EKRG+ F+LTLTG D+HR  P  I S+L  L Q K+Q++ GSVLITT HGKFFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGEDEHRFHPDTIASVLSLLEQAKSQSTKGSVLITTGHGKFFSNG 60

Query: 66  FDLPWALAAGSVSAARDRLKHIIEIFKPLIAELLSLPMPTIAAITGHAAAAGFTLALSHD 125
           FDL WA +AG    A  R+  +++ FKP++A LL LPMPTIAA+ GHAAA+G   ALSHD
Sbjct: 61  FDLAWAQSAGH--GAIKRMHQMVKSFKPVLAALLDLPMPTIAALNGHAAASGLMFALSHD 120

Query: 126 YLIMRSDRGVLYMRELDLGLTLPDYFMALFQSKTGSSLAMRDLILRGMKVRGEAAVRMGI 185
           Y+ MR DRGVLYM E+D+GL +PDYF AL  +K GS +A R+L+L G K++GE AV +GI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLPVPDYFSALVVAKVGSGIARRELLLSGKKLKGEEAVALGI 180

Query: 186 VES-AHHGQDGVVSAAVRLGEQLARRKWDGEVYAEIRKSLYPEVSQVLGLLGKAISISKL 245
           V+S AH   +GVV A V LGE LA +KW+GEVYA IRKSLYPE+ +++ L    ++   L
Sbjct: 181 VDSAAHDSAEGVVEATVSLGESLAAKKWNGEVYATIRKSLYPELCRMVDLTANNLATHNL 238

[Close]

BLAST of Lsi05G017030 vs. TAIR10
Match: AT1G65520.1 (AT1G65520.1 delta(3), delta(2)-enoyl CoA isomerase 1)

HSP 1 Score: 213.4 bits (542), Expect = 1.6e-55
Identity = 112/224 (50.00%), Postives = 157/224 (70.09%), Query Frame = 1


Query: 6   MCTIEKRGNFFILTLTGNDDHRLGPSLINSILKALSQVKAQAS-AGSVLITTSHGKFFSN 65
           MC++EKR   FIL LTG+ +HRL P+L++S+   ++Q+++  S + SVLITTS GKFFSN
Sbjct: 1   MCSLEKRDRLFILKLTGDGEHRLNPTLLDSLRSTINQIRSDPSFSQSVLITTSDGKFFSN 60

Query: 66  GFDLPWALAAGSVSAARDRLKHIIEIFKPLIAELLSLPMPTIAAITGHAAAAGFTLALSH 125
           G+DL  A +  S+S   D         + L+A+L+SLPMPTIAA+TGHA+AAG  LA+SH
Sbjct: 61  GYDLALAESNPSLSVVMDAK------LRSLVADLISLPMPTIAAVTGHASAAGCILAMSH 120

Query: 126 DYLIMRSDRGVLYMRELDLGLTLPDYFMALFQSKTGSSLAMRDLILRGMKVRGEAAVRMG 185
           DY++MR DRG LYM ELD+ L +P +FMA+ + K GS  A RD++L   KV  +  V+MG
Sbjct: 121 DYVLMRRDRGFLYMSELDIELIVPAWFMAVIRGKIGSPAARRDVMLTAAKVTADVGVKMG 180

Query: 186 IVESAHHGQDGVVSAAVRLGEQLARRKWDGEVYAEIRKSLYPEV 229
           IV+SA+      V AA++LGE++ +R  DG VY ++R+SL  EV
Sbjct: 181 IVDSAYGSAAETVEAAIKLGEEIVQRGGDGHVYGKMRESLLREV 218

[Close]

BLAST of Lsi05G017030 vs. NCBI nr
Match: gi|449432584|ref|XP_004134079.1| (PREDICTED: 3-hydroxybutyryl-CoA dehydratase-like protein, mitochondrial [Cucumis sativus])

HSP 1 Score: 416.4 bits (1069), Expect = 3.5e-113
Identity = 214/239 (89.54%), Postives = 226/239 (94.56%), Query Frame = 1


Query: 6   MCTIEKRGNFFILTLTGNDDHRLGPSLINSILKALSQVKAQASAGSVLITTSHGKFFSNG 65
           MCT+EKRGNFFILTLTG+DDHRL PSLINSILKALSQVKAQAS  SVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLSPSLINSILKALSQVKAQASPASVLITTSHGKFFSNG 60

Query: 66  FDLPWALAAGSVSAARDRLKHIIEIFKPLIAELLSLPMPTIAAITGHAAAAGFTLALSHD 125
           FDLPWAL+AGSVSAAR RL H+IEIFK ++AEL+SLPMPTIAAITGHAAAAGFTLALSHD
Sbjct: 61  FDLPWALSAGSVSAARHRLNHLIEIFKSVVAELISLPMPTIAAITGHAAAAGFTLALSHD 120

Query: 126 YLIMRSDRGVLYMRELDLGLTLPDYFMALFQSKTGSSLAMRDLILRGMKVRGEAAVRMGI 185
           YLIMRSDRGVLYMRELDLGLTLPDYFMALF+SK GSSLAMRDLILRGMKVRGEAAVRMGI
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKIGSSLAMRDLILRGMKVRGEAAVRMGI 180

Query: 186 VESAHHGQDGVVSAAVRLGEQLARRKWDGEVYAEIRKSLYPEVSQVLGLLGKAISISKL 245
           V+SAH G+D VV+AAVRLGEQLA R WDGEVYAEIRKSLYPEVS+VLGL GK ISISKL
Sbjct: 181 VDSAHDGEDSVVNAAVRLGEQLAGRNWDGEVYAEIRKSLYPEVSEVLGLPGKPISISKL 239

[Close]

BLAST of Lsi05G017030 vs. NCBI nr
Match: gi|659076193|ref|XP_008438552.1| (PREDICTED: enoyl-CoA hydratase domain-containing protein 2, mitochondrial-like [Cucumis melo])

HSP 1 Score: 416.0 bits (1068), Expect = 4.5e-113
Identity = 214/239 (89.54%), Postives = 225/239 (94.14%), Query Frame = 1


Query: 6   MCTIEKRGNFFILTLTGNDDHRLGPSLINSILKALSQVKAQASAGSVLITTSHGKFFSNG 65
           MCT+EKRGNFFILTLTG+DDHRL PSLINSILKALSQVKAQAS  SVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLSPSLINSILKALSQVKAQASPASVLITTSHGKFFSNG 60

Query: 66  FDLPWALAAGSVSAARDRLKHIIEIFKPLIAELLSLPMPTIAAITGHAAAAGFTLALSHD 125
           FDLPWALAAGSVSAAR RL HIIEIFK ++AEL+SLPMPTIAAITGHAAAAGFTLALSHD
Sbjct: 61  FDLPWALAAGSVSAARHRLNHIIEIFKSVVAELISLPMPTIAAITGHAAAAGFTLALSHD 120

Query: 126 YLIMRSDRGVLYMRELDLGLTLPDYFMALFQSKTGSSLAMRDLILRGMKVRGEAAVRMGI 185
           YLIMRSDRGVLYMRELDLGLTLPDYFMALF+SK GSSLAMRDLILRGMKVRGEAAVRMGI
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKIGSSLAMRDLILRGMKVRGEAAVRMGI 180

Query: 186 VESAHHGQDGVVSAAVRLGEQLARRKWDGEVYAEIRKSLYPEVSQVLGLLGKAISISKL 245
           V+SAHH +  VV+AAVRLGEQL  R WDGEVYAEIRKSLYPE+S+VLGL GKAISISKL
Sbjct: 181 VDSAHHSEVSVVNAAVRLGEQLVGRNWDGEVYAEIRKSLYPEISEVLGLPGKAISISKL 239

[Close]

BLAST of Lsi05G017030 vs. NCBI nr
Match: gi|449432586|ref|XP_004134080.1| (PREDICTED: 3-hydroxybutyryl-CoA dehydratase-like protein, mitochondrial [Cucumis sativus])

HSP 1 Score: 364.0 bits (933), Expect = 2.0e-97
Identity = 182/239 (76.15%), Postives = 213/239 (89.12%), Query Frame = 1


Query: 6   MCTIEKRGNFFILTLTGNDDHRLGPSLINSILKALSQVKAQASAGSVLITTSHGKFFSNG 65
           MCT+EKR N FILTLTG+DDHR  PS+I +IL+ALS+VK+QAS GSVLITTSHGKFFSNG
Sbjct: 1   MCTLEKRRNLFILTLTGHDDHRFSPSVIAAILEALSKVKSQASTGSVLITTSHGKFFSNG 60

Query: 66  FDLPWALAAGSVSAARDRLKHIIEIFKPLIAELLSLPMPTIAAITGHAAAAGFTLALSHD 125
           FDL WA AAGSVSAA +RL H+++IFKP++AELLSLPMPTIAAI+GHAAAAGF LALSHD
Sbjct: 61  FDLSWAQAAGSVSAAAERLHHMVQIFKPVVAELLSLPMPTIAAISGHAAAAGFLLALSHD 120

Query: 126 YLIMRSDRGVLYMRELDLGLTLPDYFMALFQSKTGSSLAMRDLILRGMKVRGEAAVRMGI 185
           YL+MRSDRGVLYM E+DLGL+LPDYF ALF+SK  SS   RD+++RGMKV+GEAAV+MGI
Sbjct: 121 YLLMRSDRGVLYMSEVDLGLSLPDYFAALFKSKIASSSVRRDVLMRGMKVKGEAAVKMGI 180

Query: 186 VESAHHGQDGVVSAAVRLGEQLARRKWDGEVYAEIRKSLYPEVSQVLGLLGKAISISKL 245
            +SAH G++GV+ AAVRLGEQL  RKW+GE YAEIRKSLYPE+S++LGL  KAISISKL
Sbjct: 181 ADSAHDGENGVMEAAVRLGEQLGERKWNGEPYAEIRKSLYPEISRLLGLPEKAISISKL 239

[Close]

BLAST of Lsi05G017030 vs. NCBI nr
Match: gi|659076208|ref|XP_008438558.1| (PREDICTED: enoyl-CoA hydratase domain-containing protein 2, mitochondrial-like [Cucumis melo])

HSP 1 Score: 358.2 bits (918), Expect = 1.1e-95
Identity = 181/239 (75.73%), Postives = 212/239 (88.70%), Query Frame = 1


Query: 6   MCTIEKRGNFFILTLTGNDDHRLGPSLINSILKALSQVKAQASAGSVLITTSHGKFFSNG 65
           MCT+EKR N FILTLTG+ DHRL PS+I ++L+ALS+VK+QAS GSVLITTSHGKFFSNG
Sbjct: 1   MCTLEKRRNLFILTLTGDHDHRLNPSVIAALLEALSKVKSQASPGSVLITTSHGKFFSNG 60

Query: 66  FDLPWALAAGSVSAARDRLKHIIEIFKPLIAELLSLPMPTIAAITGHAAAAGFTLALSHD 125
           FDL WA AAGSVSAA +RL H+++ FKP+IAELLSLPMPTIAAI+GHAAAAGF LALSHD
Sbjct: 61  FDLSWAHAAGSVSAAAERLHHMVQNFKPVIAELLSLPMPTIAAISGHAAAAGFMLALSHD 120

Query: 126 YLIMRSDRGVLYMRELDLGLTLPDYFMALFQSKTGSSLAMRDLILRGMKVRGEAAVRMGI 185
           YL+MRSDRGVLYM E+DLGL+LPDYF ALF+SK GSS   RD+++RGMKV+GEAAV+MGI
Sbjct: 121 YLLMRSDRGVLYMSEVDLGLSLPDYFAALFKSKIGSSSVRRDVLMRGMKVKGEAAVKMGI 180

Query: 186 VESAHHGQDGVVSAAVRLGEQLARRKWDGEVYAEIRKSLYPEVSQVLGLLGKAISISKL 245
            +SA+ G+ GV+ AAVRLGEQL  RKW+GE YAEIRKSLYPE+S++LGL  KAISISKL
Sbjct: 181 ADSAYDGESGVMEAAVRLGEQLGARKWNGEPYAEIRKSLYPEISRLLGLPEKAISISKL 239

[Close]

BLAST of Lsi05G017030 vs. NCBI nr
Match: gi|449432588|ref|XP_004134081.1| (PREDICTED: 3-hydroxybutyryl-CoA dehydratase-like protein, mitochondrial [Cucumis sativus])

HSP 1 Score: 345.1 bits (884), Expect = 9.8e-92
Identity = 175/241 (72.61%), Postives = 202/241 (83.82%), Query Frame = 1


Query: 6   MCTIEKRGNFFILTLTGNDDHRLGPSLINSILKALSQVKAQ--ASAGSVLITTSHGKFFS 65
           MC++EKRG  FILTLTGN +H L PSLINSIL ALSQVKA+  AS GSVLITTS GKFFS
Sbjct: 1   MCSMEKRGKLFILTLTGNHEHWLNPSLINSILNALSQVKAEARASPGSVLITTSQGKFFS 60

Query: 66  NGFDLPWALAAGSVSAARDRLKHIIEIFKPLIAELLSLPMPTIAAITGHAAAAGFTLALS 125
           NG DLPW L+A S+SAA +RL H+I++FKPL+A LL LP+PTIAA+ GHAAA G  LAL+
Sbjct: 61  NGLDLPWILSASSISAAHNRLNHMIQLFKPLLAHLLCLPIPTIAALPGHAAAGGLVLALA 120

Query: 126 HDYLIMRSDRGVLYMRELDLGLTLPDYFMALFQSKTGSSLAMRDLILRGMKVRGEAAVRM 185
           HDYL+MRSDRGVLYM ELDLG TLPDYF+AL +SK GSS   RD+ LRGMKVRGE AV+M
Sbjct: 121 HDYLVMRSDRGVLYMSELDLGATLPDYFIALAKSKIGSSSVRRDVFLRGMKVRGETAVKM 180

Query: 186 GIVESAHHGQDGVVSAAVRLGEQLARRKWDGEVYAEIRKSLYPEVSQVLGLLGKAISISK 245
           G+ ESAH G+DGV+ AAVRLGE+LA R WDG+VYAEIRKSLYPE+S +LGL  K I+ISK
Sbjct: 181 GLAESAHQGEDGVMEAAVRLGEELAARNWDGDVYAEIRKSLYPEISGLLGLTTKVITISK 240

The following BLAST results are available for this feature:

BLAST of Lsi05G017030 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ECI2_ARATH	6.2e-78	60.42	Enoyl-CoA delta isomerase 2, peroxisomal OS=Arabidopsis thaliana GN=ECI2 PE=1 SV...	[more]
ECI3_ARATH	3.5e-73	57.92	Enoyl-CoA delta isomerase 3 OS=Arabidopsis thaliana GN=ECI3 PE=1 SV=1	[more]
ECI1_ARATH	2.8e-54	50.00	Enoyl-CoA delta isomerase 1, peroxisomal OS=Arabidopsis thaliana GN=ECI1 PE=1 SV...	[more]

BLAST of Lsi05G017030 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L9X4_CUCSA	2.4e-113	89.54	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G141770 PE=4 SV=1	[more]
A0A0A0L8C7_CUCSA	1.4e-97	76.15	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G141780 PE=4 SV=1	[more]
M5XMU9_PRUPE	2.9e-82	67.25	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa010715mg PE=4 SV=1	[more]
A0A061EMT3_THECC	4.9e-82	62.34	Indole-3-butyric acid response 10 OS=Theobroma cacao GN=TCM_020837 PE=4 SV=1	[more]
F6H8I1_VITVI	1.9e-81	64.02	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_05s0049g00180 PE=4 SV=...	[more]

BLAST of Lsi05G017030 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G14430.1	3.5e-79	60.42	indole-3-butyric acid response 10	[more]
AT4G14440.1	2.0e-74	57.92	3-hydroxyacyl-CoA dehydratase 1	[more]
AT1G65520.1	1.6e-55	50.00	delta(3), delta(2)-enoyl CoA isomerase 1	[more]

BLAST of Lsi05G017030 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449432584\|ref\|XP_004134079.1\|	3.5e-113	89.54	PREDICTED: 3-hydroxybutyryl-CoA dehydratase-like protein, mitochondrial [Cucumis...	[more]
gi\|659076193\|ref\|XP_008438552.1\|	4.5e-113	89.54	PREDICTED: enoyl-CoA hydratase domain-containing protein 2, mitochondrial-like [...	[more]
gi\|449432586\|ref\|XP_004134080.1\|	2.0e-97	76.15	PREDICTED: 3-hydroxybutyryl-CoA dehydratase-like protein, mitochondrial [Cucumis...	[more]
gi\|659076208\|ref\|XP_008438558.1\|	1.1e-95	75.73	PREDICTED: enoyl-CoA hydratase domain-containing protein 2, mitochondrial-like [...	[more]
gi\|449432588\|ref\|XP_004134081.1\|	9.8e-92	72.61	PREDICTED: 3-hydroxybutyryl-CoA dehydratase-like protein, mitochondrial [Cucumis...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity

Vocabulary: **INTERPRO**
Term	Definition
IPR001753	Enoyl-CoA_hydra/iso

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
cellular_component	GO:0016020	membrane
molecular_function	GO:0003824	catalytic activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G017030.1	Lsi05G017030.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001753	Crotonase superfamily	PFAM	PF00378	ECH_1	coord: 30..211 score: 1.0
None	No IPR available	PANTHER	PTHR11941	ENOYL-COA HYDRATASE-RELATED	coord: 6..228 score: 3.6E
None	No IPR available	PANTHER	PTHR11941:SF84	SUBFAMILY NOT NAMED	coord: 6..228 score: 3.6E

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi05G017030	Watermelon (97103) v2	lsiwmbB333
Lsi05G017030	Watermelon (97103) v2	lsiwmbB341
Lsi05G017030	Watermelon (97103) v2	lsiwmbB347
Lsi05G017030	Wax gourd	lsiwgoB421
Lsi05G017030	Wax gourd	lsiwgoB462
Lsi05G017030	Wax gourd	lsiwgoB466
Lsi05G017030	Bottle gourd (USVL1VR-Ls)	lsilsiB011
Lsi05G017030	Cucumber (Gy14) v1	cgylsiB415
Lsi05G017030	Cucumber (Gy14) v1	cgylsiB620
Lsi05G017030	Cucurbita maxima (Rimu)	cmalsiB009
Lsi05G017030	Cucurbita maxima (Rimu)	cmalsiB245
Lsi05G017030	Cucurbita maxima (Rimu)	cmalsiB356
Lsi05G017030	Cucurbita maxima (Rimu)	cmalsiB586
Lsi05G017030	Cucurbita maxima (Rimu)	cmalsiB764
Lsi05G017030	Cucurbita maxima (Rimu)	cmalsiB839
Lsi05G017030	Cucurbita moschata (Rifu)	cmolsiB233
Lsi05G017030	Cucurbita moschata (Rifu)	cmolsiB345
Lsi05G017030	Cucurbita moschata (Rifu)	cmolsiB757
Lsi05G017030	Cucurbita moschata (Rifu)	cmolsiB823
Lsi05G017030	Cucurbita pepo (Zucchini)	cpelsiB267
Lsi05G017030	Cucurbita pepo (Zucchini)	cpelsiB506
Lsi05G017030	Cucurbita pepo (Zucchini)	cpelsiB617
Lsi05G017030	Cucurbita pepo (Zucchini)	cpelsiB714
Lsi05G017030	Wild cucumber (PI 183967)	cpilsiB125
Lsi05G017030	Wild cucumber (PI 183967)	cpilsiB207
Lsi05G017030	Cucumber (Chinese Long) v2	culsiB127
Lsi05G017030	Cucumber (Chinese Long) v2	culsiB209
Lsi05G017030	Melon (DHL92) v3.5.1	lsimeB360
Lsi05G017030	Melon (DHL92) v3.5.1	lsimeB378
Lsi05G017030	Watermelon (Charleston Gray)	lsiwcgB335
Lsi05G017030	Watermelon (Charleston Gray)	lsiwcgB343
Lsi05G017030	Watermelon (Charleston Gray)	lsiwcgB349
Lsi05G017030	Watermelon (97103) v1	lsiwmB359
Lsi05G017030	Watermelon (97103) v1	lsiwmB365
Lsi05G017030	Watermelon (97103) v1	lsiwmB382
Lsi05G017030	Cucumber (Gy14) v2	cgyblsiB115
Lsi05G017030	Cucumber (Gy14) v2	cgyblsiB196
Lsi05G017030	Melon (DHL92) v3.6.1	lsimedB375
Lsi05G017030	Melon (DHL92) v3.6.1	lsimedB396
Lsi05G017030	Silver-seed gourd	carlsiB206
Lsi05G017030	Silver-seed gourd	carlsiB210
Lsi05G017030	Silver-seed gourd	carlsiB718
Lsi05G017030	Silver-seed gourd	carlsiB878
Lsi05G017030	Silver-seed gourd	carlsiB983
Lsi05G017030	Cucumber (Chinese Long) v3	cuclsiB139
Lsi05G017030	Cucumber (Chinese Long) v3	cuclsiB228