Lsi05G014450 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi05G014450
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionDehydrogenase/reductase SDR family protein 7-like
Locationchr05 : 22337774 .. 22340658 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTCTTTTTCTTTTTGAAACAGAGTCAAAGTTTAGGCATTGGTTATAATTTAGCGTTGTTATTAATGCGAAAAGGAATCAGAAATTGTAATAATCATCAACAACAAGACACATCGTTTACTTCTATTCTATACTACAAAGCTTTCATCAATGGCGGACTTCAATTACGCCTTCATCTCTCTCCTTCTTCTTCTTCCTTTCGCACTCCTCGTTTTCCTATACCTAATTGTGCGTCCACGTCCCGTTAAGATTCATCTGAAGAATCGCCATGTCTTCATTACTGGCGGATCGAGCGGAATCGGCCTCGCTTTGGCCCACCAGGCAGCGTCGGAGGGCGCGCGAGTCTCCATCCTTTCGCGTTCTCTCAAGAAGCTCGAGGAGGCGAAAGACGCAATCCGACTCTCCTCTGGCATTGATGTGGCGGTCTACGCTGCCGATGTTCGAGATTACTATGCCATTTTGAAGGCTGTAGATGAAGCAGGCCCAATTGACGTTCTTATTGTGAATCAAGGCGTGTTCGTACCTCAAGAACTGGCGGAGCAGGAATTGGATGAGGTGAAATTCATGTTAGATGTGAATTTATTGGGCACATTTAACATGATTAAGGCTGCCCTACCTGCGATGAAGAATAGAGTGGACCGTCGACCCGCATCGATCGCTCTCATGTCTTCTCAGGCTGGTCAGGTAATTGATATAAAGGATGGCTAAGTGAGGAGCGTGAATTGTTTACTGTTACAGATATGTAGGGTGATCTTGGTTATAGAAAATACTTTTGAGTGGTTTTATGAAGTGCTAATGTTCGAAACTTATACTCTGGAGTGAACTTCTTCAATTATTAGGGTTAATTCAATGTCATTGATAACATGCAGACATTCGCAAGTTGTGAATTTAGTAGAATGGTAAATCTTTTACTCATGAAAGTCTTCTAATAAATTTGTGTAGGTGGGTATTTATGGTTACACGGCGTATTCAGCTAGTAAGTTTGGGATTCGAGGTTTGGCAGAAGCCTTGCAACAGGAGGTCATTGGAGATGACATACATATTTCTCTTATATTCCCTCCAGACACCGACACTCCTGGATTTGCACAAGGTATGTGTAAAGCAAGACCCGACTTACCTTATAGTTTTGTTCTGCTAGTCCTCAATTAGGGGTTTCAATATTTATCCGGTGGGTTGGAATATTTTTAGCTTAGTTTTCAGTCATTGGTTTGTATGAAGATATGATGGGAAATATCTTGGAAGGATAAGCTAACTAGACTATCACCTGGCTAGAATTTATTTGAGTTAGTAACCTTATTTATTGGATATCTCGGCCTAACAAAAACCGTTAGATTTATACTACAGAATGTTTTGTTAGCTGGTTTCTTCAGTTATAAGTTGCTTATCCTTAATTGCAGAGCAAAAGAAAAGGCCTGAATTGACCACTATCATTGCTGCTGGAGGAGGCAGTATGAAACCAGAGTTAGTTGCCAAAAAAGCTTTTAATGGCATTAAATCTGGTAGTTTTATAATTCCCTGCAACTTTGAGGGAGCATTATTGTCCATAGCAACTGCTGGCTTATCTCCTCAGAGATCATTCTTAATGGCATTCCTTGAAGTTGTTGGTGCTGGAGTAGTAAGGATTGTGGCATTGTGTTTTCAATGGTTTTGGTATGGAAGTATTGAAAAATGGCATGCACAAAGAAATAGTAAGTCTTCATTTAAATGATGAAACCATCATCTTGTTCAGTCCATTATTAATATATGTGCTAAAATTGCTTCAGGTGTCTAAAAGCTCAATGTGTGGTTGACTATTAAAAATGGCATTTGTTGAGATCAGGCAGCAATGAGATTTGGTTAAAATAGTCAAGCTTTTACACATTTCAAAAGCTAATTAGATTTCTTTTTTTGTTTTTAGTTACATAACTATGCAATCTCTGGTTCTTGGTTTCATTTTTGTATTTTGTTTTAAGATAACGATCTTCTTTCTCTTTTAATTTCTAACATATAATGATGTGAATGACTCTGTCTTCCTTCTGAACTTTTGCTTCAACGGCTCTATTGTGGATGTCATATTAGATCAGTCTTCTAATTATCAAATCTTTAACATATATTCTCAGACTGTTGGTGTCTGTTACGTCTTGTCTTAAGCTGTGAATTGACCAGTTACCTACCTAAATAACAGAAAGTCTACATTTAACTCAAGAAATTTAATTATGTTATAGTGAGGTCAAATATCTGGATAATCCTGAAACTTGACACTTTCATCAAAGTTCATTTCTTTGTTTGTTCCTTTCTCTCTAATTGTAACTTCAAGCCATTTGATGACCATGTGTTAGTAAACTTATCTATATCCTTTTGCCAGGAACGTAGAAGATACGTCTGGTTATTTCTCGGCAGTTTCTTTCATTTATGGCTATTAGCTATGCAAGTTATCAACTCTGGATTCCAAAATAAGTGGAAGTAAAGGCTCGGTCGCGCCAGACGAGTTGAACACCATTGCTCGACAGTTTAAATATTTGGTAGGGAAAAGGATCAGATGACTGATTCCGTAGCAGTTCTCTCTCTTCCCCTTTGCTTGGCGGCCTGTAATTTTCAGAGTCTTGACAGAAAGGATTTCACGCGGTGTGTTTACTCTTTTCCTCTCTTTTTTTTTGGTTAGATCGGCTGCTGGAACTGGATTTTCGTTCAACTTCTTGAATTAAGTATAGGGACATAATTTGATCATGTGATCAAGTTTGGACCTTTATAAGCTTATCTTGCCACAAACGGCAGCATTAGAGATTTGTTTAGTAAATTAGTTGTTATTATTATTTATTGATTTTTTTAAATGAAAAATTTGCTTATAATTTTTTTTTCTTTTTTGTTGAAAAGCTCATAAAAAGAGCCCAACTATAAGAAATGGCA

mRNA sequence

TTTCTTTTTCTTTTTGAAACAGAGTCAAAGTTTAGGCATTGGTTATAATTTAGCGTTGTTATTAATGCGAAAAGGAATCAGAAATTGTAATAATCATCAACAACAAGACACATCGTTTACTTCTATTCTATACTACAAAGCTTTCATCAATGGCGGACTTCAATTACGCCTTCATCTCTCTCCTTCTTCTTCTTCCTTTCGCACTCCTCGTTTTCCTATACCTAATTGTGCGTCCACGTCCCGTTAAGATTCATCTGAAGAATCGCCATGTCTTCATTACTGGCGGATCGAGCGGAATCGGCCTCGCTTTGGCCCACCAGGCAGCGTCGGAGGGCGCGCGAGTCTCCATCCTTTCGCGTTCTCTCAAGAAGCTCGAGGAGGCGAAAGACGCAATCCGACTCTCCTCTGGCATTGATGTGGCGGTCTACGCTGCCGATGTTCGAGATTACTATGCCATTTTGAAGGCTGTAGATGAAGCAGGCCCAATTGACGTTCTTATTGTGAATCAAGGCGTGTTCGTACCTCAAGAACTGGCGGAGCAGGAATTGGATGAGGTGAAATTCATGTTAGATGTGAATTTATTGGGCACATTTAACATGATTAAGGCTGCCCTACCTGCGATGAAGAATAGAGTGGACCGTCGACCCGCATCGATCGCTCTCATGTCTTCTCAGGCTGGTCAGGTGGGTATTTATGGTTACACGGCGTATTCAGCTAGTAAGTTTGGGATTCGAGGTTTGGCAGAAGCCTTGCAACAGGAGGTCATTGGAGATGACATACATATTTCTCTTATATTCCCTCCAGACACCGACACTCCTGGATTTGCACAAGAGCAAAAGAAAAGGCCTGAATTGACCACTATCATTGCTGCTGGAGGAGGCAGTATGAAACCAGAGTTAGTTGCCAAAAAAGCTTTTAATGGCATTAAATCTGGTAGTTTTATAATTCCCTGCAACTTTGAGGGAGCATTATTGTCCATAGCAACTGCTGGCTTATCTCCTCAGAGATCATTCTTAATGGCATTCCTTGAAGTTGTTGGTGCTGGAGTAGTAAGGATTGTGGCATTGTGTTTTCAATGGTTTTGGAACGTAGAAGATACGTCTGGTTATTTCTCGGCAGTTTCTTTCATTTATGGCTATTAGCTATGCAAGTTATCAACTCTGGATTCCAAAATAAGTGGAAGTAAAGGCTCGGTCGCGCCAGACGAGTTGAACACCATTGCTCGACAGTTTAAATATTTGGTAGGGAAAAGGATCAGATGACTGATTCCGTAGCAGTTCTCTCTCTTCCCCTTTGCTTGGCGGCCTGTAATTTTCAGAGTCTTGACAGAAAGGATTTCACGCGGTGTGTTTACTCTTTTCCTCTCTTTTTTTTTGGTTAGATCGGCTGCTGGAACTGGATTTTCGTTCAACTTCTTGAATTAAGTATAGGGACATAATTTGATCATGTGATCAAGTTTGGACCTTTATAAGCTTATCTTGCCACAAACGGCAGCATTAGAGATTTGTTTAGTAAATTAGTTGTTATTATTATTTATTGATTTTTTTAAATGAAAAATTTGCTTATAATTTTTTTTTCTTTTTTGTTGAAAAGCTCATAAAAAGAGCCCAACTATAAGAAATGGCA

Coding sequence (CDS)

ATGGCGGACTTCAATTACGCCTTCATCTCTCTCCTTCTTCTTCTTCCTTTCGCACTCCTCGTTTTCCTATACCTAATTGTGCGTCCACGTCCCGTTAAGATTCATCTGAAGAATCGCCATGTCTTCATTACTGGCGGATCGAGCGGAATCGGCCTCGCTTTGGCCCACCAGGCAGCGTCGGAGGGCGCGCGAGTCTCCATCCTTTCGCGTTCTCTCAAGAAGCTCGAGGAGGCGAAAGACGCAATCCGACTCTCCTCTGGCATTGATGTGGCGGTCTACGCTGCCGATGTTCGAGATTACTATGCCATTTTGAAGGCTGTAGATGAAGCAGGCCCAATTGACGTTCTTATTGTGAATCAAGGCGTGTTCGTACCTCAAGAACTGGCGGAGCAGGAATTGGATGAGGTGAAATTCATGTTAGATGTGAATTTATTGGGCACATTTAACATGATTAAGGCTGCCCTACCTGCGATGAAGAATAGAGTGGACCGTCGACCCGCATCGATCGCTCTCATGTCTTCTCAGGCTGGTCAGGTGGGTATTTATGGTTACACGGCGTATTCAGCTAGTAAGTTTGGGATTCGAGGTTTGGCAGAAGCCTTGCAACAGGAGGTCATTGGAGATGACATACATATTTCTCTTATATTCCCTCCAGACACCGACACTCCTGGATTTGCACAAGAGCAAAAGAAAAGGCCTGAATTGACCACTATCATTGCTGCTGGAGGAGGCAGTATGAAACCAGAGTTAGTTGCCAAAAAAGCTTTTAATGGCATTAAATCTGGTAGTTTTATAATTCCCTGCAACTTTGAGGGAGCATTATTGTCCATAGCAACTGCTGGCTTATCTCCTCAGAGATCATTCTTAATGGCATTCCTTGAAGTTGTTGGTGCTGGAGTAGTAAGGATTGTGGCATTGTGTTTTCAATGGTTTTGGAACGTAGAAGATACGTCTGGTTATTTCTCGGCAGTTTCTTTCATTTATGGCTATTAG

Protein sequence

MADFNYAFISLLLLLPFALLVFLYLIVRPRPVKIHLKNRHVFITGGSSGIGLALAHQAASEGARVSILSRSLKKLEEAKDAIRLSSGIDVAVYAADVRDYYAILKAVDEAGPIDVLIVNQGVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVGIYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAQEQKKRPELTTIIAAGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGALLSIATAGLSPQRSFLMAFLEVVGAGVVRIVALCFQWFWNVEDTSGYFSAVSFIYGY
Homology
[Close]
BLAST of Lsi05G014450 vs. Swiss-Prot
Match: TC10A_ARATH (3-dehydrosphinganine reductase TSC10A OS=Arabidopsis thaliana GN=TSC10A PE=1 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 1.5e-114
Identity = 212/313 (67.73%), Postives = 265/313 (84.66%), Query Frame = 1 

Query: 1   MADFNYAFISLLL-LLPFALLVFLYLIVRPRPVKIHLKNRHVFITGGSSGIGLALAHQAA 60
           MA  +  F+  L+ ++P +LL  L LIVRPRP+KI +K+RH FITGGSSGIGLALAH+AA
Sbjct: 1   MAAISPLFLLFLIPIIPLSLLAILALIVRPRPIKIPIKSRHAFITGGSSGIGLALAHRAA 60

Query: 61  SEGARVSILSRSLKKLEEAKDAIRLSSGIDVAVYAADVRDYYAILKAVDEAGPIDVLIVN 120
           SEGARVSIL+RS  KLEEAK +I+L++G++VA ++ADVRDY A+ KA+DE+GPIDVLIVN
Sbjct: 61  SEGARVSILARSGSKLEEAKKSIQLATGVEVATFSADVRDYDAVSKAIDESGPIDVLIVN 120

Query: 121 QGVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQV 180
           QGVF  +EL +   ++VKF +DVNL+G+FN+IKAALPAMK R DR PASI+L+SSQAGQV
Sbjct: 121 QGVFTAKELVKHSPEDVKFTIDVNLVGSFNVIKAALPAMKARKDRGPASISLVSSQAGQV 180

Query: 181 GIYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAQEQKKRPELTTII 240
           G+YGY AYSASKFG++GLA+ALQQEVI DDIH++LIFPPDT+TPGF +EQK RPE+T II
Sbjct: 181 GVYGYAAYSASKFGLQGLAQALQQEVISDDIHVTLIFPPDTNTPGFEEEQKSRPEVTAII 240

Query: 241 AAGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGALLSIATAGLSPQRSFLMAFLEVVGAG 300
           AA  GSM+ E VAKKA +GIK+G+F + CNFEG LLS+AT G+SPQRSF +AFLEV+ AG
Sbjct: 241 AASSGSMETEEVAKKAMDGIKAGNFTVSCNFEGFLLSLATTGMSPQRSFWLAFLEVITAG 300

Query: 301 VVRIVALCFQWFW 313
            +R++AL FQW W
Sbjct: 301 PIRLIALFFQWDW 313

[Close]
BLAST of Lsi05G014450 vs. Swiss-Prot
Match: TC10B_ARATH (3-dehydrosphinganine reductase TSC10B OS=Arabidopsis thaliana GN=TSC10B PE=1 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 1.2e-105
Identity = 200/307 (65.15%), Postives = 249/307 (81.11%), Query Frame = 1 

Query: 6   YAFISLLLLLPFALLVFLYLIVRPRPVKIHLKNRHVFITGGSSGIGLALAHQAASEGARV 65
           ++     +L   +LL+ L  IVRPR V I +K RHVFITGGSSGIGLALAH+A SEGA+V
Sbjct: 5   FSLFLFFILFIVSLLIILSFIVRPRSVTIPIKFRHVFITGGSSGIGLALAHRAVSEGAKV 64

Query: 66  SILSRSLKKLEEAKDAIRLSSGIDVAVYAADVRDYYAILKAVDEAGPIDVLIVNQGVFVP 125
           SIL+RS +KL EAK +I+L++G++VA ++ADVRDY A+ KA+DE+GPIDVLIVNQGVF+ 
Sbjct: 65  SILARSTEKLAEAKRSIQLATGVEVATFSADVRDYDAVSKAIDESGPIDVLIVNQGVFIG 124

Query: 126 QELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVGIYGYT 185
           +EL +Q  +EVKFM+DVNL G+FN+IKAALPAMK R  R PASI+L+SSQAGQ GIYGYT
Sbjct: 125 KELEKQSPEEVKFMIDVNLTGSFNVIKAALPAMKAREGRGPASISLVSSQAGQAGIYGYT 184

Query: 186 AYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAQEQKKRPELTTIIAAGGGS 245
           AYSASKFG++GLA+ALQQEVI D IH++L+FPPDTDTPGF QE KKRPELT+IIAA  GS
Sbjct: 185 AYSASKFGLQGLAQALQQEVISDGIHVTLLFPPDTDTPGFEQELKKRPELTSIIAASSGS 244

Query: 246 MKPELVAKKAFNGIKSGSFIIPCNFEGALLSIATAGLSPQRSFLMAFLEVVGAGVVRIVA 305
           MK   VAK  F+GIK+G F + C+F G LLSIA+ G+SPQ SF +A  EV+  G++R+ +
Sbjct: 245 MKTNEVAKICFDGIKAGKFTVTCHFIGFLLSIASTGMSPQGSFWLALTEVMFGGLIRLAS 304

Query: 306 LCFQWFW 313
           L FQW W
Sbjct: 305 LVFQWQW 311

[Close]
BLAST of Lsi05G014450 vs. Swiss-Prot
Match: KDSR_HUMAN (3-ketodihydrosphingosine reductase OS=Homo sapiens GN=KDSR PE=1 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 3.0e-43
Identity = 113/309 (36.57%), Postives = 180/309 (58.25%), Query Frame = 1 

Query: 9   ISLLLLLPFALLVFLYL-IVRPRPVKIHLKNRHVFITGGSSGIGLALAHQAASEGARVSI 68
           ++   L+ F LL+++   ++ P+P+   L   HV +TGGSSGIG  +A +   +GA +++
Sbjct: 4   LAAAFLVAFVLLLYMVSPLISPKPLA--LPGAHVVVTGGSSGIGKCIAIECYKQGAFITL 63

Query: 69  LSRSLKKLEEAKDAIRLSSGID---VAVYAADVRDYY-----AILKAVDEAGPIDVLIVN 128
           ++R+  KL +AK  I + S  D   V   + DV   Y      I +A ++ GP+D+L+  
Sbjct: 64  VARNEDKLLQAKKEIEMHSINDKQVVLCISVDVSQDYNQVENVIKQAQEKLGPVDMLVNC 123

Query: 129 QGVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQV 188
            G+ V  +  + E+   + ++ +N LG+    +A +  MK   +RR   I  +SSQAGQ+
Sbjct: 124 AGMAVSGKFEDLEVSTFERLMSINYLGSVYPSRAVITTMK---ERRVGRIVFVSSQAGQL 183

Query: 189 GIYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAQEQKKRPELTTII 248
           G++G+TAYSASKF IRGLAEALQ EV   +++I++ +PPDTDTPGFA+E + +P  T +I
Sbjct: 184 GLFGFTAYSASKFAIRGLAEALQMEVKPYNVYITVAYPPDTDTPGFAEENRTKPLETRLI 243

Query: 249 AAGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGALLSIATAGLSPQRSFLMAFLEVVGAG 308
           +      KPE VAK+       G+F      +G +LS  T G++P  S      +VV  G
Sbjct: 244 SETTSVCKPEQVAKQIVKDAIQGNFNSSLGSDGYMLSALTCGMAPVTSITEGLQQVVTMG 303

[Close]
BLAST of Lsi05G014450 vs. Swiss-Prot
Match: KDSR_BOVIN (3-ketodihydrosphingosine reductase OS=Bos taurus GN=KDSR PE=2 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 3.3e-42
Identity = 109/304 (35.86%), Postives = 181/304 (59.54%), Query Frame = 1 

Query: 14  LLPFALLVFLYL-IVRPRPVKIHLKNRHVFITGGSSGIGLALAHQAASEGARVSILSRSL 73
           L+ F LL+++   ++ P+P+   L   HV +TGGSSGIG  +A +   +GA +++++R+ 
Sbjct: 9   LVAFVLLLYMVSPLISPKPLA--LPGAHVVVTGGSSGIGKCIAIECYKQGAFITLVARNE 68

Query: 74  KKLEEAKDAIRLSSGID---VAVYAADVRDYYA-----ILKAVDEAGPIDVLIVNQGVFV 133
            KL +AK  I   S  D   V   + DV   Y+     I +A ++ GP+D+L+   G+ +
Sbjct: 69  DKLLQAKKEIEKHSINDKQVVLCISVDVSQDYSQVENVIKQAQEKLGPVDMLVNCAGMSL 128

Query: 134 PQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVGIYGY 193
             +  + E+   + ++ +N LG+    +A +  MK   +RR   +  +SSQAGQ+G++GY
Sbjct: 129 SGKFEDLEVSTFERLMSINYLGSVYPSRAVIATMK---ERRMGRVVFVSSQAGQLGLFGY 188

Query: 194 TAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAQEQKKRPELTTIIAAGGG 253
           TAYS+SKF +RGLAEALQ EV   ++++++ +PPDTDTPGFA+E + +P  T +I+    
Sbjct: 189 TAYSSSKFALRGLAEALQMEVKPYNVYVTVAYPPDTDTPGFAKENQTKPLETRLISETTS 248

Query: 254 SMKPELVAKKAFNGIKSGSFIIPCNFEGALLSIATAGLSPQRSFLMAFLEVVGAGVVRIV 309
             KPE VAK+    ++ G+F      +G +LS  T G++P  S +    +VV  G+ R +
Sbjct: 249 VCKPEQVAKQIVKDVQ-GNFNSSIGSDGYMLSSLTCGMAPVTSIMEGLQQVVTMGLFRTI 306

[Close]
BLAST of Lsi05G014450 vs. Swiss-Prot
Match: KDSR_MOUSE (3-ketodihydrosphingosine reductase OS=Mus musculus GN=Kdsr PE=1 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 4.3e-42
Identity = 111/304 (36.51%), Postives = 175/304 (57.57%), Query Frame = 1 

Query: 14  LLPFALLVFLYL-IVRPRPVKIHLKNRHVFITGGSSGIGLALAHQAASEGARVSILSRSL 73
           L+ F LL+++   ++ P+P+   L   HV +TGGSSGIG  +A +   +GA +++++R+ 
Sbjct: 9   LVAFVLLLYMVSPLISPKPLA--LPGAHVVVTGGSSGIGKCIAIECYKQGAFITLVARNE 68

Query: 74  KKLEEAKDAIRLSSGID---VAVYAADVRDYY-----AILKAVDEAGPIDVLIVNQGVFV 133
            KL +AK  I   S  D   V   + DV   Y      I +A ++ GP+D+L+   G  +
Sbjct: 69  DKLLQAKKDIEKHSINDKQVVLCISVDVSQDYNQVENVIKQAQEKLGPVDMLVNCAGTSM 128

Query: 134 PQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVGIYGY 193
             +  E E+   + ++ +N LG+    +A +  MK   +RR   I  +SSQAGQ+G++G+
Sbjct: 129 SGKFEELEVSSFEKLMSINYLGSVYPSRAVITTMK---ERRVGRIVFVSSQAGQLGLFGF 188

Query: 194 TAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAQEQKKRPELTTIIAAGGG 253
           TAYS+SKF IRGLAEALQ EV   ++++++ +PPDTDTPG A+E K +P  T +I+    
Sbjct: 189 TAYSSSKFAIRGLAEALQMEVKPYNVYVTVAYPPDTDTPGLAEENKTKPLETRLISETTA 248

Query: 254 SMKPELVAKKAFNGIKSGSFIIPCNFEGALLSIATAGLSPQRSFLMAFLEVVGAGVVRIV 309
             KPE VAK+       G+F      +G +LS  T G++P  S      +VV  G+ R +
Sbjct: 249 ICKPEQVAKQIVKDAIQGNFNSSIGSDGYMLSSLTCGMAPVTSITEGLQQVVTMGLFRTI 307

[Close]
BLAST of Lsi05G014450 vs. TrEMBL
Match: A0A0A0L506_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G149900 PE=4 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 6.3e-157
Identity = 294/312 (94.23%), Postives = 305/312 (97.76%), Query Frame = 1 

Query: 1   MADFNYAFISLLLLLPFALLVFLYLIVRPRPVKIHLKNRHVFITGGSSGIGLALAHQAAS 60
           MAD N AFISL+LLLPFALL+FLYLIVRPRPV+I LK+RHVFITGGSSGIGLALAHQAA+
Sbjct: 1   MADLNLAFISLVLLLPFALLLFLYLIVRPRPVRISLKSRHVFITGGSSGIGLALAHQAAT 60

Query: 61  EGARVSILSRSLKKLEEAKDAIRLSSGIDVAVYAADVRDYYAILKAVDEAGPIDVLIVNQ 120
           EGARVSIL+RSLKKLEEAKDAIRLS+GIDVAVYAADVRDY AILKAVDEAGPIDVLIVNQ
Sbjct: 61  EGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAILKAVDEAGPIDVLIVNQ 120

Query: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG 180
           GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG
Sbjct: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG 180

Query: 181 IYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAQEQKKRPELTTIIA 240
           IYGYTAYSASKFGIRGLAEALQQEVIGDDIH+SLIFPPDTDTPGFA+EQKKRPELTTIIA
Sbjct: 181 IYGYTAYSASKFGIRGLAEALQQEVIGDDIHVSLIFPPDTDTPGFAEEQKKRPELTTIIA 240

Query: 241 AGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGALLSIATAGLSPQRSFLMAFLEVVGAGV 300
           AGGGSMKPELVAKKAFNGIKSGSFIIPCNFEG LLSIATAGLSPQRSF MAF+EVVGAGV
Sbjct: 241 AGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRSFFMAFIEVVGAGV 300

Query: 301 VRIVALCFQWFW 313
           +RIVALCFQW W
Sbjct: 301 IRIVALCFQWVW 312

[Close]
BLAST of Lsi05G014450 vs. TrEMBL
Match: A0A067L7Q6_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_02122 PE=4 SV=1)

HSP 1 Score: 462.2 bits (1188), Expect = 5.2e-127
Identity = 240/312 (76.92%), Postives = 279/312 (89.42%), Query Frame = 1 

Query: 1   MADFNYAFISLLLLLPFALLVFLYLIVRPRPVKIHLKNRHVFITGGSSGIGLALAHQAAS 60
           MAD N   + LLL++P +LL  L LIVRPRPVKI +KNRHVFITGGSSGIGLALA +AAS
Sbjct: 1   MADLNLLLL-LLLVIPISLLALLILIVRPRPVKIPIKNRHVFITGGSSGIGLALAIRAAS 60

Query: 61  EGARVSILSRSLKKLEEAKDAIRLSSGIDVAVYAADVRDYYAILKAVDEAGPIDVLIVNQ 120
           EGARVSIL+RSL KLEEAK  IRLS+G+DVA++AADVRD+ ++ KAV EAGPIDVL+VNQ
Sbjct: 61  EGARVSILARSLDKLEEAKHFIRLSTGVDVAIFAADVRDFDSVTKAVVEAGPIDVLVVNQ 120

Query: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG 180
           GVF+PQEL +QELDEV+FM+DVNL+G+FNMIKAALP MK R +RRPASIALMSSQAGQVG
Sbjct: 121 GVFLPQELEKQELDEVRFMIDVNLMGSFNMIKAALPGMKIRDERRPASIALMSSQAGQVG 180

Query: 181 IYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAQEQKKRPELTTIIA 240
           IYGYTAYSASKFG+RGLAEALQQEVI D+IH+SLIFPPDTDTP  A+E K+RP+LT+IIA
Sbjct: 181 IYGYTAYSASKFGLRGLAEALQQEVIADNIHVSLIFPPDTDTPALAEENKRRPQLTSIIA 240

Query: 241 AGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGALLSIATAGLSPQRSFLMAFLEVVGAGV 300
           A  G+MK + VAKKA +GIKSGSF++PCNFEGALLSIATAGLSPQRSFLMAF+EVV A +
Sbjct: 241 ASSGAMKADEVAKKALDGIKSGSFVVPCNFEGALLSIATAGLSPQRSFLMAFIEVVAASM 300

Query: 301 VRIVALCFQWFW 313
           +R++ALCFQW W
Sbjct: 301 IRLLALCFQWNW 311

[Close]
BLAST of Lsi05G014450 vs. TrEMBL
Match: A0A0V0IAT6_SOLCH (Putative 3-ketodihydrosphingosine reductase-like OS=Solanum chacoense PE=4 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 3.4e-126
Identity = 232/313 (74.12%), Postives = 278/313 (88.82%), Query Frame = 1 

Query: 1   MADFNYAFISLLLLL-PFALLVFLYLIVRPRPVKIHLKNRHVFITGGSSGIGLALAHQAA 60
           MAD + AF SLLLL    +LLVFL  IVRPRPVK+ +KNRHVFITGGSSGIGLALA QAA
Sbjct: 1   MADLSIAFFSLLLLFFSLSLLVFLAFIVRPRPVKVPIKNRHVFITGGSSGIGLALAQQAA 60

Query: 61  SEGARVSILSRSLKKLEEAKDAIRLSSGIDVAVYAADVRDYYAILKAVDEAGPIDVLIVN 120
           SEGA+VSIL+R+  +LE+AK++IRLS+G DVA+++ADVRDY A+ KAV+EAGPIDVL+ N
Sbjct: 61  SEGAKVSILARNTGRLEDAKESIRLSTGRDVAIFSADVRDYEAVKKAVEEAGPIDVLVCN 120

Query: 121 QGVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQV 180
           QGVFVPQEL  Q+++E+KFM+DVNL GTF++IKAALP MKNR DR P SIA+MSSQAGQV
Sbjct: 121 QGVFVPQELETQDIEEIKFMIDVNLTGTFHLIKAALPGMKNRADRGPGSIAIMSSQAGQV 180

Query: 181 GIYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAQEQKKRPELTTII 240
           GIYGYTAYSASKFG++GLAEALQQEVIG++IH+SLIFPPDT+TPGFA+E K+RP+LT+I+
Sbjct: 181 GIYGYTAYSASKFGLKGLAEALQQEVIGENIHVSLIFPPDTETPGFAEENKRRPQLTSIL 240

Query: 241 AAGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGALLSIATAGLSPQRSFLMAFLEVVGAG 300
           AA  GSMK + VAKKA NG+KSG+FI+PCNFEG  LSIATAGLSPQRSFLMAF+EV+ AG
Sbjct: 241 AASSGSMKADEVAKKALNGLKSGNFIVPCNFEGFFLSIATAGLSPQRSFLMAFIEVIAAG 300

Query: 301 VVRIVALCFQWFW 313
           ++R+V LCFQW W
Sbjct: 301 ILRVVGLCFQWNW 313

[Close]
BLAST of Lsi05G014450 vs. TrEMBL
Match: A0A061ECT9_THECC (Short-chain dehydrogenase, putative OS=Theobroma cacao GN=TCM_017113 PE=4 SV=1)

HSP 1 Score: 453.0 bits (1164), Expect = 3.2e-124
Identity = 231/301 (76.74%), Postives = 266/301 (88.37%), Query Frame = 1 

Query: 12  LLLLPFALLVFLYLIVRPRPVKIHLKNRHVFITGGSSGIGLALAHQAASEGARVSILSRS 71
           L+L PF LL+ LY IVRPRPV I +KNRHVFITGGSSGIGLALAHQAAS+GARVS+L+RS
Sbjct: 6   LVLFPF-LLLLLYFIVRPRPVNIPIKNRHVFITGGSSGIGLALAHQAASQGARVSLLARS 65

Query: 72  LKKLEEAKDAIRLSSGIDVAVYAADVRDYYAILKAVDEAGPIDVLIVNQGVFVPQELAEQ 131
           L KLEEAK +IRLSSG+DV+++AADVRDYYA+ KA+++AGPIDVL+VNQGVFVPQEL +Q
Sbjct: 66  LSKLEEAKQSIRLSSGVDVSIFAADVRDYYAVQKAINDAGPIDVLVVNQGVFVPQELEKQ 125

Query: 132 ELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVGIYGYTAYSASK 191
            L+EVKFM+DVNL+G+FN+IKA LP MK R DR P SIALMSSQAGQVG+YGYTAYSASK
Sbjct: 126 GLEEVKFMIDVNLMGSFNVIKAVLPLMKERKDRGPTSIALMSSQAGQVGVYGYTAYSASK 185

Query: 192 FGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAQEQKKRPELTTIIAAGGGSMKPELV 251
           FG+RGLAEALQQEVI D+IH++LIFPPDTDTPGF QE K RPELTTI+A   GSMK + V
Sbjct: 186 FGLRGLAEALQQEVIADNIHVTLIFPPDTDTPGFEQESKVRPELTTILAGSSGSMKADKV 245

Query: 252 AKKAFNGIKSGSFIIPCNFEGALLSIATAGLSPQRSFLMAFLEVVGAGVVRIVALCFQWF 311
           AKKA +GIKSGSFI+PCNFEG +L+IATAGLSPQRSFLMAF EV   G+ R+VAL FQW 
Sbjct: 246 AKKALDGIKSGSFIVPCNFEGQMLAIATAGLSPQRSFLMAFFEVAFIGICRLVALFFQWN 305

Query: 312 W 313
           W
Sbjct: 306 W 305

[Close]
BLAST of Lsi05G014450 vs. TrEMBL
Match: W9RHG1_9ROSA (3-ketodihydrosphingosine reductase OS=Morus notabilis GN=L484_007343 PE=4 SV=1)

HSP 1 Score: 452.6 bits (1163), Expect = 4.1e-124
Identity = 237/312 (75.96%), Postives = 269/312 (86.22%), Query Frame = 1 

Query: 1   MADFNYAFISLLLLLPFALLVFLYLIVRPRPVKIHLKNRHVFITGGSSGIGLALAHQAAS 60
           MAD N A+  LL +LP ALLVFLY IVRPRPV+I +KNRHVFITGGSSGIGLALAH+AAS
Sbjct: 1   MADLNLAY--LLFVLPLALLVFLYFIVRPRPVRIPIKNRHVFITGGSSGIGLALAHRAAS 60

Query: 61  EGARVSILSRSLKKLEEAKDAIRLSSGIDVAVYAADVRDYYAILKAVDEAGPIDVLIVNQ 120
           EGARVSIL+RS  KLE+AK AIRL++G+DVA +AADVRD  A+ +A +EAGPIDVL+VNQ
Sbjct: 61  EGARVSILARSHDKLEDAKHAIRLATGVDVATFAADVRDADAVRRAAEEAGPIDVLVVNQ 120

Query: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG 180
           GVFVP+EL +Q LDEVKFM+DVNL+G+FNMIKAALP MKNR  R P SIALMSSQAGQVG
Sbjct: 121 GVFVPEELEKQGLDEVKFMVDVNLIGSFNMIKAALPEMKNREGRGPGSIALMSSQAGQVG 180

Query: 181 IYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAQEQKKRPELTTIIA 240
           IYGYTAYSASKFG+RGLAE+LQ E+I +DIHISLIFPPDT+TPGF +E KKRP LT II 
Sbjct: 181 IYGYTAYSASKFGLRGLAESLQHELIAEDIHISLIFPPDTNTPGFIEESKKRPPLTRIIT 240

Query: 241 AGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGALLSIATAGLSPQRSFLMAFLEVVGAGV 300
              G MK E VAKKA +GIK GSFIIPCNFEG LLSIATAGLSPQRSF MAF+EV+ AG+
Sbjct: 241 GSSGEMKAEEVAKKALDGIKCGSFIIPCNFEGLLLSIATAGLSPQRSFPMAFVEVLTAGL 300

Query: 301 VRIVALCFQWFW 313
           +R+ ALCFQW W
Sbjct: 301 IRVAALCFQWNW 310

[Close]
BLAST of Lsi05G014450 vs. TAIR10
Match: AT3G06060.1 (AT3G06060.1 NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 414.1 bits (1063), Expect = 8.2e-116
Identity = 212/313 (67.73%), Postives = 265/313 (84.66%), Query Frame = 1 

Query: 1   MADFNYAFISLLL-LLPFALLVFLYLIVRPRPVKIHLKNRHVFITGGSSGIGLALAHQAA 60
           MA  +  F+  L+ ++P +LL  L LIVRPRP+KI +K+RH FITGGSSGIGLALAH+AA
Sbjct: 1   MAAISPLFLLFLIPIIPLSLLAILALIVRPRPIKIPIKSRHAFITGGSSGIGLALAHRAA 60

Query: 61  SEGARVSILSRSLKKLEEAKDAIRLSSGIDVAVYAADVRDYYAILKAVDEAGPIDVLIVN 120
           SEGARVSIL+RS  KLEEAK +I+L++G++VA ++ADVRDY A+ KA+DE+GPIDVLIVN
Sbjct: 61  SEGARVSILARSGSKLEEAKKSIQLATGVEVATFSADVRDYDAVSKAIDESGPIDVLIVN 120

Query: 121 QGVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQV 180
           QGVF  +EL +   ++VKF +DVNL+G+FN+IKAALPAMK R DR PASI+L+SSQAGQV
Sbjct: 121 QGVFTAKELVKHSPEDVKFTIDVNLVGSFNVIKAALPAMKARKDRGPASISLVSSQAGQV 180

Query: 181 GIYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAQEQKKRPELTTII 240
           G+YGY AYSASKFG++GLA+ALQQEVI DDIH++LIFPPDT+TPGF +EQK RPE+T II
Sbjct: 181 GVYGYAAYSASKFGLQGLAQALQQEVISDDIHVTLIFPPDTNTPGFEEEQKSRPEVTAII 240

Query: 241 AAGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGALLSIATAGLSPQRSFLMAFLEVVGAG 300
           AA  GSM+ E VAKKA +GIK+G+F + CNFEG LLS+AT G+SPQRSF +AFLEV+ AG
Sbjct: 241 AASSGSMETEEVAKKAMDGIKAGNFTVSCNFEGFLLSLATTGMSPQRSFWLAFLEVITAG 300

Query: 301 VVRIVALCFQWFW 313
            +R++AL FQW W
Sbjct: 301 PIRLIALFFQWDW 313

[Close]
BLAST of Lsi05G014450 vs. TAIR10
Match: AT5G19200.1 (AT5G19200.1 NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 384.4 bits (986), Expect = 7.0e-107
Identity = 200/307 (65.15%), Postives = 249/307 (81.11%), Query Frame = 1 

Query: 6   YAFISLLLLLPFALLVFLYLIVRPRPVKIHLKNRHVFITGGSSGIGLALAHQAASEGARV 65
           ++     +L   +LL+ L  IVRPR V I +K RHVFITGGSSGIGLALAH+A SEGA+V
Sbjct: 5   FSLFLFFILFIVSLLIILSFIVRPRSVTIPIKFRHVFITGGSSGIGLALAHRAVSEGAKV 64

Query: 66  SILSRSLKKLEEAKDAIRLSSGIDVAVYAADVRDYYAILKAVDEAGPIDVLIVNQGVFVP 125
           SIL+RS +KL EAK +I+L++G++VA ++ADVRDY A+ KA+DE+GPIDVLIVNQGVF+ 
Sbjct: 65  SILARSTEKLAEAKRSIQLATGVEVATFSADVRDYDAVSKAIDESGPIDVLIVNQGVFIG 124

Query: 126 QELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVGIYGYT 185
           +EL +Q  +EVKFM+DVNL G+FN+IKAALPAMK R  R PASI+L+SSQAGQ GIYGYT
Sbjct: 125 KELEKQSPEEVKFMIDVNLTGSFNVIKAALPAMKAREGRGPASISLVSSQAGQAGIYGYT 184

Query: 186 AYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAQEQKKRPELTTIIAAGGGS 245
           AYSASKFG++GLA+ALQQEVI D IH++L+FPPDTDTPGF QE KKRPELT+IIAA  GS
Sbjct: 185 AYSASKFGLQGLAQALQQEVISDGIHVTLLFPPDTDTPGFEQELKKRPELTSIIAASSGS 244

Query: 246 MKPELVAKKAFNGIKSGSFIIPCNFEGALLSIATAGLSPQRSFLMAFLEVVGAGVVRIVA 305
           MK   VAK  F+GIK+G F + C+F G LLSIA+ G+SPQ SF +A  EV+  G++R+ +
Sbjct: 245 MKTNEVAKICFDGIKAGKFTVTCHFIGFLLSIASTGMSPQGSFWLALTEVMFGGLIRLAS 304

Query: 306 LCFQWFW 313
           L FQW W
Sbjct: 305 LVFQWQW 311

[Close]
BLAST of Lsi05G014450 vs. TAIR10
Match: AT3G26760.1 (AT3G26760.1 NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 78.2 bits (191), Expect = 1.1e-14
Identity = 69/223 (30.94%), Postives = 105/223 (47.09%), Query Frame = 1 

Query: 36  LKNRHVFITGGSSGIGLALAHQAASEGARVSILSRSLKKLEEAKDAIRLSSGIDVAVYAA 95
           L+ +   ITGG+SGIG A A +  S+GA+V I+       EEA   +    G        
Sbjct: 36  LEGKVAVITGGASGIGKATAEEFVSQGAQVIIVDID----EEAGHMVATELGSAAHFLRC 95

Query: 96  DVRDYYAILKAVDEA----GPIDVLIVNQGV---FVPQELAEQELDEVKFMLDVNLLGTF 155
           DV +   I KAV+ A    G +DV++ + G+     P  +A+ ++D    ++ +N+ GT 
Sbjct: 96  DVTEEEQIAKAVETAVTRHGKLDVMLNSAGISCSISPPSIADLDMDTYDKVMRLNVRGTV 155

Query: 156 NMIKAALPAMKNRVDRRPASIALMSSQAGQVGIYGYTAYSASKFGIRGLAEALQQEVIGD 215
             IK A  AM   +     SI  +SS +G +G  G  AYS SKF I G+ + +  E+   
Sbjct: 156 LGIKHAARAM---IPAGSGSILCLSSISGLMGGLGPHAYSISKFTIPGVVKTVASELCKH 215

Query: 216 DIHISLIFPPDTDTP--------GFAQEQKKRPELTTIIAAGG 244
            + I+ I P    TP         FA    +  +L  I+ A G
Sbjct: 216 GLRINCISPAGIPTPLTLRMFREAFAGHSIREEQLLAIVNATG 251

[Close]
BLAST of Lsi05G014450 vs. TAIR10
Match: AT3G47350.2 (AT3G47350.2 hydroxysteroid dehydrogenase 2)

HSP 1 Score: 67.0 bits (162), Expect = 2.5e-11
Identity = 66/231 (28.57%), Postives = 113/231 (48.92%), Query Frame = 1 

Query: 11  LLLLLPFALLVFLYLIVRPRPVKIHLKN---RHVFITGGSSGIGLALAHQAASEGARVSI 70
           L+L  PF L   L   ++     +H +N   + V ITG SSGIG  +A++ A +GA++++
Sbjct: 20  LVLFYPFYLFTKLMSCLK----HLHFENVTGKVVLITGASSGIGEHVAYEYAKKGAKLAL 79

Query: 71  LSRSLKKLEEAKDAIRLSSGIDVAVYAADVRDYYAILKAVDEA----GPIDVLIVNQGVF 130
           ++R   +LE   +  R     DV +   DV +     K +DE     G +D LI N G  
Sbjct: 80  VARRKDRLEIVAETSRQLGSGDVIIIPGDVSNVEDCKKFIDETIHHFGKLDHLINNAG-- 139

Query: 131 VPQELAEQELDEVK---FMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG 190
           VPQ +  ++  +++    ++D+N  G+  +   A+P ++    +    I ++SS    + 
Sbjct: 140 VPQTVIFEDFTQIQDANSIMDINFWGSTYITYFAIPHLR----KSKGKIVVISSATAIIP 199

Query: 191 IYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIFP----PDTDTPGFAQ 228
           +   + YSASK  +    E L+ E I  DI I++  P     D  TP F +
Sbjct: 200 LQAASVYSASKAALVKFFETLRVE-ISPDIKITIALPGFISTDMTTPQFKE 239

[Close]
BLAST of Lsi05G014450 vs. TAIR10
Match: AT3G47360.1 (AT3G47360.1 hydroxysteroid dehydrogenase 3)

HSP 1 Score: 66.6 bits (161), Expect = 3.2e-11
Identity = 60/214 (28.04%), Postives = 106/214 (49.53%), Query Frame = 1 

Query: 9   ISLLLLLPFALLVFLYLIVRPRPVKIHLKNRHVFITGGSSGIGLALAHQAASEGARVSIL 68
           I L L  PF LL+ L L +R      ++  + V ITG SSGIG  +A++ A +GA ++++
Sbjct: 18  IFLFLFYPFYLLIKLVLCLRKNLHFENVARKVVLITGASSGIGEHVAYEYAKKGAYLALV 77

Query: 69  SRSLKKLEEAKDAIRLSSGIDVAVYAADVRDYYAILKAVDEA----GPIDVLIVNQGVFV 128
           +R   +LE   +  R     +V +   DV +     K +DE     G +D LI N GVF 
Sbjct: 78  ARRRDRLEIVAETSRQLGSGNVIIIPGDVSNVEDCKKFIDETIRHFGKLDHLINNAGVFQ 137

Query: 129 PQELAE-QELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVGIYG 188
                +  ++ +   ++D+N  GT  +   A+P ++    +    I  ++S +  + +  
Sbjct: 138 TVLFEDFTQIQDANPIMDINFWGTTYITYFAIPHLR----KSKGKIVAITSGSANIPLPL 197

Query: 189 YTAYSASKFGIRGLAEALQQEVIGDDIHISLIFP 218
            + Y+ASK  +    E L+ E +  DI I+++ P
Sbjct: 198 ASIYAASKAALLRFFETLRIE-LSPDIKITIVLP 226

[Close]
BLAST of Lsi05G014450 vs. NCBI nr
Match: gi|659076487|ref|XP_008438707.1| (PREDICTED: 3-ketodihydrosphingosine reductase [Cucumis melo])

HSP 1 Score: 562.0 bits (1447), Expect = 6.9e-157
Identity = 295/312 (94.55%), Postives = 305/312 (97.76%), Query Frame = 1 

Query: 1   MADFNYAFISLLLLLPFALLVFLYLIVRPRPVKIHLKNRHVFITGGSSGIGLALAHQAAS 60
           MAD N+AFISL+LLLP ALL+FLYLIVRPRPVKI LK+RHVFITGGSSGIGLALAHQAA+
Sbjct: 1   MADLNFAFISLILLLPLALLLFLYLIVRPRPVKIPLKSRHVFITGGSSGIGLALAHQAAT 60

Query: 61  EGARVSILSRSLKKLEEAKDAIRLSSGIDVAVYAADVRDYYAILKAVDEAGPIDVLIVNQ 120
           EGARVSIL+RSLKKLEEAKDAIRLS+GIDVAVYAADVRDY AI KAVDEAGPIDVLIVNQ
Sbjct: 61  EGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAISKAVDEAGPIDVLIVNQ 120

Query: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG 180
           GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG
Sbjct: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG 180

Query: 181 IYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAQEQKKRPELTTIIA 240
           IYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFA+EQKKRPELTTIIA
Sbjct: 181 IYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAEEQKKRPELTTIIA 240

Query: 241 AGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGALLSIATAGLSPQRSFLMAFLEVVGAGV 300
           AGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGALLS+ATAGLSPQRSF MAFLEVVGAGV
Sbjct: 241 AGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGALLSVATAGLSPQRSFFMAFLEVVGAGV 300

Query: 301 VRIVALCFQWFW 313
           +RIVALCFQW W
Sbjct: 301 IRIVALCFQWVW 312

[Close]
BLAST of Lsi05G014450 vs. NCBI nr
Match: gi|449432720|ref|XP_004134147.1| (PREDICTED: 3-dehydrosphinganine reductase TSC10A [Cucumis sativus])

HSP 1 Score: 561.6 bits (1446), Expect = 9.0e-157
Identity = 294/312 (94.23%), Postives = 305/312 (97.76%), Query Frame = 1 

Query: 1   MADFNYAFISLLLLLPFALLVFLYLIVRPRPVKIHLKNRHVFITGGSSGIGLALAHQAAS 60
           MAD N AFISL+LLLPFALL+FLYLIVRPRPV+I LK+RHVFITGGSSGIGLALAHQAA+
Sbjct: 1   MADLNLAFISLVLLLPFALLLFLYLIVRPRPVRISLKSRHVFITGGSSGIGLALAHQAAT 60

Query: 61  EGARVSILSRSLKKLEEAKDAIRLSSGIDVAVYAADVRDYYAILKAVDEAGPIDVLIVNQ 120
           EGARVSIL+RSLKKLEEAKDAIRLS+GIDVAVYAADVRDY AILKAVDEAGPIDVLIVNQ
Sbjct: 61  EGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAILKAVDEAGPIDVLIVNQ 120

Query: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG 180
           GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG
Sbjct: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG 180

Query: 181 IYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAQEQKKRPELTTIIA 240
           IYGYTAYSASKFGIRGLAEALQQEVIGDDIH+SLIFPPDTDTPGFA+EQKKRPELTTIIA
Sbjct: 181 IYGYTAYSASKFGIRGLAEALQQEVIGDDIHVSLIFPPDTDTPGFAEEQKKRPELTTIIA 240

Query: 241 AGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGALLSIATAGLSPQRSFLMAFLEVVGAGV 300
           AGGGSMKPELVAKKAFNGIKSGSFIIPCNFEG LLSIATAGLSPQRSF MAF+EVVGAGV
Sbjct: 241 AGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRSFFMAFIEVVGAGV 300

Query: 301 VRIVALCFQWFW 313
           +RIVALCFQW W
Sbjct: 301 IRIVALCFQWVW 312

[Close]
BLAST of Lsi05G014450 vs. NCBI nr
Match: gi|643733177|gb|KDP40124.1| (hypothetical protein JCGZ_02122 [Jatropha curcas])

HSP 1 Score: 462.2 bits (1188), Expect = 7.5e-127
Identity = 240/312 (76.92%), Postives = 279/312 (89.42%), Query Frame = 1 

Query: 1   MADFNYAFISLLLLLPFALLVFLYLIVRPRPVKIHLKNRHVFITGGSSGIGLALAHQAAS 60
           MAD N   + LLL++P +LL  L LIVRPRPVKI +KNRHVFITGGSSGIGLALA +AAS
Sbjct: 1   MADLNLLLL-LLLVIPISLLALLILIVRPRPVKIPIKNRHVFITGGSSGIGLALAIRAAS 60

Query: 61  EGARVSILSRSLKKLEEAKDAIRLSSGIDVAVYAADVRDYYAILKAVDEAGPIDVLIVNQ 120
           EGARVSIL+RSL KLEEAK  IRLS+G+DVA++AADVRD+ ++ KAV EAGPIDVL+VNQ
Sbjct: 61  EGARVSILARSLDKLEEAKHFIRLSTGVDVAIFAADVRDFDSVTKAVVEAGPIDVLVVNQ 120

Query: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG 180
           GVF+PQEL +QELDEV+FM+DVNL+G+FNMIKAALP MK R +RRPASIALMSSQAGQVG
Sbjct: 121 GVFLPQELEKQELDEVRFMIDVNLMGSFNMIKAALPGMKIRDERRPASIALMSSQAGQVG 180

Query: 181 IYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAQEQKKRPELTTIIA 240
           IYGYTAYSASKFG+RGLAEALQQEVI D+IH+SLIFPPDTDTP  A+E K+RP+LT+IIA
Sbjct: 181 IYGYTAYSASKFGLRGLAEALQQEVIADNIHVSLIFPPDTDTPALAEENKRRPQLTSIIA 240

Query: 241 AGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGALLSIATAGLSPQRSFLMAFLEVVGAGV 300
           A  G+MK + VAKKA +GIKSGSF++PCNFEGALLSIATAGLSPQRSFLMAF+EVV A +
Sbjct: 241 ASSGAMKADEVAKKALDGIKSGSFVVPCNFEGALLSIATAGLSPQRSFLMAFIEVVAASM 300

Query: 301 VRIVALCFQWFW 313
           +R++ALCFQW W
Sbjct: 301 IRLLALCFQWNW 311

[Close]
BLAST of Lsi05G014450 vs. NCBI nr
Match: gi|802580345|ref|XP_012069554.1| (PREDICTED: somatic embryogenesis receptor kinase 1-like isoform X2 [Jatropha curcas])

HSP 1 Score: 462.2 bits (1188), Expect = 7.5e-127
Identity = 240/312 (76.92%), Postives = 279/312 (89.42%), Query Frame = 1 

Query: 1   MADFNYAFISLLLLLPFALLVFLYLIVRPRPVKIHLKNRHVFITGGSSGIGLALAHQAAS 60
           MAD N   + LLL++P +LL  L LIVRPRPVKI +KNRHVFITGGSSGIGLALA +AAS
Sbjct: 40  MADLNLLLL-LLLVIPISLLALLILIVRPRPVKIPIKNRHVFITGGSSGIGLALAIRAAS 99

Query: 61  EGARVSILSRSLKKLEEAKDAIRLSSGIDVAVYAADVRDYYAILKAVDEAGPIDVLIVNQ 120
           EGARVSIL+RSL KLEEAK  IRLS+G+DVA++AADVRD+ ++ KAV EAGPIDVL+VNQ
Sbjct: 100 EGARVSILARSLDKLEEAKHFIRLSTGVDVAIFAADVRDFDSVTKAVVEAGPIDVLVVNQ 159

Query: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG 180
           GVF+PQEL +QELDEV+FM+DVNL+G+FNMIKAALP MK R +RRPASIALMSSQAGQVG
Sbjct: 160 GVFLPQELEKQELDEVRFMIDVNLMGSFNMIKAALPGMKIRDERRPASIALMSSQAGQVG 219

Query: 181 IYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAQEQKKRPELTTIIA 240
           IYGYTAYSASKFG+RGLAEALQQEVI D+IH+SLIFPPDTDTP  A+E K+RP+LT+IIA
Sbjct: 220 IYGYTAYSASKFGLRGLAEALQQEVIADNIHVSLIFPPDTDTPALAEENKRRPQLTSIIA 279

Query: 241 AGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGALLSIATAGLSPQRSFLMAFLEVVGAGV 300
           A  G+MK + VAKKA +GIKSGSF++PCNFEGALLSIATAGLSPQRSFLMAF+EVV A +
Sbjct: 280 ASSGAMKADEVAKKALDGIKSGSFVVPCNFEGALLSIATAGLSPQRSFLMAFIEVVAASM 339

Query: 301 VRIVALCFQWFW 313
           +R++ALCFQW W
Sbjct: 340 IRLLALCFQWNW 350

[Close]
BLAST of Lsi05G014450 vs. NCBI nr
Match: gi|802580343|ref|XP_012069553.1| (PREDICTED: 3-dehydrosphinganine reductase TSC10A-like isoform X1 [Jatropha curcas])

HSP 1 Score: 462.2 bits (1188), Expect = 7.5e-127
Identity = 240/312 (76.92%), Postives = 279/312 (89.42%), Query Frame = 1 

Query: 1   MADFNYAFISLLLLLPFALLVFLYLIVRPRPVKIHLKNRHVFITGGSSGIGLALAHQAAS 60
           MAD N   + LLL++P +LL  L LIVRPRPVKI +KNRHVFITGGSSGIGLALA +AAS
Sbjct: 40  MADLNLLLL-LLLVIPISLLALLILIVRPRPVKIPIKNRHVFITGGSSGIGLALAIRAAS 99

Query: 61  EGARVSILSRSLKKLEEAKDAIRLSSGIDVAVYAADVRDYYAILKAVDEAGPIDVLIVNQ 120
           EGARVSIL+RSL KLEEAK  IRLS+G+DVA++AADVRD+ ++ KAV EAGPIDVL+VNQ
Sbjct: 100 EGARVSILARSLDKLEEAKHFIRLSTGVDVAIFAADVRDFDSVTKAVVEAGPIDVLVVNQ 159

Query: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG 180
           GVF+PQEL +QELDEV+FM+DVNL+G+FNMIKAALP MK R +RRPASIALMSSQAGQVG
Sbjct: 160 GVFLPQELEKQELDEVRFMIDVNLMGSFNMIKAALPGMKIRDERRPASIALMSSQAGQVG 219

Query: 181 IYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAQEQKKRPELTTIIA 240
           IYGYTAYSASKFG+RGLAEALQQEVI D+IH+SLIFPPDTDTP  A+E K+RP+LT+IIA
Sbjct: 220 IYGYTAYSASKFGLRGLAEALQQEVIADNIHVSLIFPPDTDTPALAEENKRRPQLTSIIA 279

Query: 241 AGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGALLSIATAGLSPQRSFLMAFLEVVGAGV 300
           A  G+MK + VAKKA +GIKSGSF++PCNFEGALLSIATAGLSPQRSFLMAF+EVV A +
Sbjct: 280 ASSGAMKADEVAKKALDGIKSGSFVVPCNFEGALLSIATAGLSPQRSFLMAFIEVVAASM 339

Query: 301 VRIVALCFQWFW 313
           +R++ALCFQW W
Sbjct: 340 IRLLALCFQWNW 350

The following BLAST results are available for this feature:
BLAST of Lsi05G014450 vs. Swiss-Prot
Match NameE-valueIdentityDescription
TC10A_ARATH1.5e-11467.733-dehydrosphinganine reductase TSC10A OS=Arabidopsis thaliana GN=TSC10A PE=1 SV=... [more]
TC10B_ARATH1.2e-10565.153-dehydrosphinganine reductase TSC10B OS=Arabidopsis thaliana GN=TSC10B PE=1 SV=... [more]
KDSR_HUMAN3.0e-4336.573-ketodihydrosphingosine reductase OS=Homo sapiens GN=KDSR PE=1 SV=1[more]
KDSR_BOVIN3.3e-4235.863-ketodihydrosphingosine reductase OS=Bos taurus GN=KDSR PE=2 SV=1[more]
KDSR_MOUSE4.3e-4236.513-ketodihydrosphingosine reductase OS=Mus musculus GN=Kdsr PE=1 SV=1[more]
BLAST of Lsi05G014450 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L506_CUCSA6.3e-15794.23Uncharacterized protein OS=Cucumis sativus GN=Csa_3G149900 PE=4 SV=1[more]
A0A067L7Q6_JATCU5.2e-12776.92Uncharacterized protein OS=Jatropha curcas GN=JCGZ_02122 PE=4 SV=1[more]
A0A0V0IAT6_SOLCH3.4e-12674.12Putative 3-ketodihydrosphingosine reductase-like OS=Solanum chacoense PE=4 SV=1[more]
A0A061ECT9_THECC3.2e-12476.74Short-chain dehydrogenase, putative OS=Theobroma cacao GN=TCM_017113 PE=4 SV=1[more]
W9RHG1_9ROSA4.1e-12475.963-ketodihydrosphingosine reductase OS=Morus notabilis GN=L484_007343 PE=4 SV=1[more]
BLAST of Lsi05G014450 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G06060.18.2e-11667.73 NAD(P)-binding Rossmann-fold superfamily protein[more]
AT5G19200.17.0e-10765.15 NAD(P)-binding Rossmann-fold superfamily protein[more]
AT3G26760.11.1e-1430.94 NAD(P)-binding Rossmann-fold superfamily protein[more]
AT3G47350.22.5e-1128.57 hydroxysteroid dehydrogenase 2[more]
AT3G47360.13.2e-1128.04 hydroxysteroid dehydrogenase 3[more]
BLAST of Lsi05G014450 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659076487|ref|XP_008438707.1|6.9e-15794.55PREDICTED: 3-ketodihydrosphingosine reductase [Cucumis melo][more]
gi|449432720|ref|XP_004134147.1|9.0e-15794.23PREDICTED: 3-dehydrosphinganine reductase TSC10A [Cucumis sativus][more]
gi|643733177|gb|KDP40124.1|7.5e-12776.92hypothetical protein JCGZ_02122 [Jatropha curcas][more]
gi|802580345|ref|XP_012069554.1|7.5e-12776.92PREDICTED: somatic embryogenesis receptor kinase 1-like isoform X2 [Jatropha cur... [more]
gi|802580343|ref|XP_012069553.1|7.5e-12776.92PREDICTED: 3-dehydrosphinganine reductase TSC10A-like isoform X1 [Jatropha curca... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
Vocabulary: INTERPRO
TermDefinition
IPR020904Sc_DH/Rdtase_CS
IPR016040NAD(P)-bd_dom
IPR002347SDR_fam
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0006687 glycosphingolipid metabolic process
biological_process GO:0006468 protein phosphorylation
biological_process GO:0008150 biological_process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005575 cellular_component
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0047560 3-dehydrosphinganine reductase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G014450.1Lsi05G014450.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 40..57
score: 1.4E-14coord: 187..206
score: 1.4E-14coord: 161..177
score: 1.4E-14coord: 111..122
score: 1.4
IPR002347Short-chain dehydrogenase/reductase SDRPFAMPF00106adh_shortcoord: 40..231
score: 8.7
IPR016040NAD(P)-binding domainGENE3DG3DSA:3.40.50.720coord: 38..263
score: 7.7
IPR016040NAD(P)-binding domainunknownSSF51735NAD(P)-binding Rossmann-fold domainscoord: 35..265
score: 1.41
IPR020904Short-chain dehydrogenase/reductase, conserved sitePROSITEPS00061ADH_SHORTcoord: 174..202
scor
NoneNo IPR availablePANTHERPTHR24320FAMILY NOT NAMEDcoord: 7..264
score: 7.6
NoneNo IPR availablePANTHERPTHR24320:SF13-KETODIHYDROSPHINGOSINE REDUCTASEcoord: 7..264
score: 7.6

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Lsi05G014450Csa3G149900Cucumber (Chinese Long) v2culsiB199
Lsi05G014450CSPI03G13380Wild cucumber (PI 183967)cpilsiB198
Lsi05G014450Cucsa.254100Cucumber (Gy14) v1cgylsiB420
Lsi05G014450CmaCh06G014270Cucurbita maxima (Rimu)cmalsiB757
Lsi05G014450Cla021652Watermelon (97103) v1lsiwmB362
Lsi05G014450MELO3C006553Melon (DHL92) v3.5.1lsimeB369
Lsi05G014450ClCG05G005330Watermelon (Charleston Gray)lsiwcgB333
Lsi05G014450MELO3C006553.2Melon (DHL92) v3.6.1lsimedB385
Lsi05G014450Cla97C05G086640Watermelon (97103) v2lsiwmbB330
Lsi05G014450Bhi01G000619Wax gourdlsiwgoB432
Lsi05G014450CsGy3G013500Cucumber (Gy14) v2cgyblsiB184
Lsi05G014450CsaV3_3G013580Cucumber (Chinese Long) v3cuclsiB218
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Lsi05G014450Bottle gourd (USVL1VR-Ls)lsilsiB006
Lsi05G014450Cucurbita maxima (Rimu)cmalsiB250
Lsi05G014450Cucurbita maxima (Rimu)cmalsiB353
Lsi05G014450Cucurbita maxima (Rimu)cmalsiB834
Lsi05G014450Cucurbita moschata (Rifu)cmolsiB238
Lsi05G014450Cucurbita moschata (Rifu)cmolsiB342
Lsi05G014450Cucurbita moschata (Rifu)cmolsiB752
Lsi05G014450Cucurbita moschata (Rifu)cmolsiB819
Lsi05G014450Cucurbita pepo (Zucchini)cpelsiB118
Lsi05G014450Cucurbita pepo (Zucchini)cpelsiB273
Lsi05G014450Cucurbita pepo (Zucchini)cpelsiB512
Lsi05G014450Cucurbita pepo (Zucchini)cpelsiB718
Lsi05G014450Melon (DHL92) v3.5.1lsimeB342
Lsi05G014450Watermelon (Charleston Gray)lsiwcgB345
Lsi05G014450Watermelon (97103) v1lsiwmB384
Lsi05G014450Melon (DHL92) v3.6.1lsimedB359
Lsi05G014450Silver-seed gourdcarlsiB065
Lsi05G014450Silver-seed gourdcarlsiB200
Lsi05G014450Silver-seed gourdcarlsiB298
Lsi05G014450Silver-seed gourdcarlsiB552
Lsi05G014450Watermelon (97103) v2lsiwmbB344
Lsi05G014450Wax gourdlsiwgoB422