Lsi05G013430 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi05G013430
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionHydroxysteroid dehydrogenase, putative
Locationchr05 : 21292540 .. 21300928 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATTTCCGCGCCAACAACTTCTCTTTCTCTCTAGCGTCGGATTCCAACCCTAACAACCTCAATGCATTTCCCTCTCAATCTCATTCTCAGTTCTCTCTTTTTTCTCTGATCTTTCCTCCTCCGATCCCACCATTAACAAATGGAGGTCGACGATCGCTTTGTTGAATTGAGTACCAAGACTTGCGTTGTGTTGGGCGGCCGTGGATTTCTCGGCCGATCGCTGGTTCTCCGGCTCCTCAAACTCGGTAAGTGGAATGTCCGTATCGCTGATTCTGCGGAGTCGCTTGAACTCGATCCATCTGAGCATGATTCTCTCCTCTCTGACGCCCTTATCGCTGGCCGAGCTTCATATTTCCATGTCGATGTTCGCAATAAGGATCAGATCGTTGCAGGTCTTTGTTTCTCTCTCTTTCTCTCTGTTTGGTTGATTGTGATATGTTGATAAGTTTTACTTCGTTTGGATTTTGTGTTGACTGGAGGAGTTAATTGAATTTTCTTTATAATGCAAAGATTCTCAGCCTTTTTCTCTGTCTATGGAACTGGTTGAATTTTAAGAGGATCGGTGTCTGGATTTAGATTGCCTTCGTGGAGTTGGAATTAAGGTTATCCTATGAAAATAATGATGGATAGAATAGAAGATGATTTGATTGTTGAGACAGTTTCCATGAACTGATGTCCAAGTCTGAAGAATTATTTGAATTATTAAAAGGCTGCTATGATAGTCGAGTGAAACTTCAAATTGTAGGTTCTTGTGTTTTTGGTTCTATGCGTGACTCTTTCCCTTTTTTTCCAATAATTTTCCGAATGTGTTTTAAAGTGATTTTCTTTTGGAATACTCTCTCCATTGGATCATAACGTTAGTATGAGAAACTGCTATGATCTTTGAATTTTGTGGAAATTTTCAACTGTATCATAGTAAAAAATTTGATGGAGGATTTTTTCTTCAGCTATCAATAGCGTTGGGAGGTTTCCCGTAAAAACATTTATCCTTTTTTTTTTTTTTTAAAGATGGAGAACCATTATTTTCTTGAAAGCTTAGAGAAATGTAGAGAGAGGCAAACTGAAAATTCCATAGTGAATCGTTAATTTTTGTGAATTTATTGTCATTCCTCATTGTTGCTACTTCCATCCATGGCTGTCTATTTTCTATCCTGAAATATGTCAATAGCTTAATTGGGTATCAAGTACTTGTGTGCCAATTTTTTTTCCTTTTGCAGCTCTCAGTGGTTCTTCCGTTGTGTTTTATATGGACTATGAAGATTCACATTCAAATGATATTTATCTCTCATATATGTTTATAGTTCAAGGTATTGAAAGCTATTAATCTTTTGAGAGAATCCTTGTTATTCTGAACATATCCAACTACGAAGAAGGATGTAATTGCTGGCATCTTGATTTCTCATTTAGGTGCCAAAAATGTCATTAATGCTTGTCGAGACTGCAAAGTAAGAAGGCTAATATTCAACAGTTCAGCTGATGTAATTTTTGATGGTTCGCGTGATATACGTAATGGAGAGGAATCATTAACATACCCATGGAAAGTATGTTTCTGCCTGAAGTGTTTCTCAATCATCTGTTTTTGGACATCCCTAACATTCTCTTCTGCTAATCATTTCCTCATCAAGTGAGTACTATGTACAGTTTGAGGACATGTTGAGTGACCTAAAGGCTCAAGCTGAAGCACTCATTCTGTTTGCCAACGATATAGATGGACTTCTAACATGTGCACTTCGCCCTAGCAATGTTTTTGGGCCAGAAGACACTCGACTTGTTCCTTTCATGGTGCATCAGGCAAAATCTGGTTTGGCAAAGGTTTGCAGAATTACTTATGTTTTAATCCACACTTTGTTTTAACTATTGTCATCGAAACTTTGTTTCTTAATATATATATATATATATATATATATATATATATATATAACTATTGTCATGGAAACTTCAGTAAAATACATCAAATATAGTGGATTGTTTGTGCTTTACCATTTATAAACTTAAGAACAATAGATCTTATAGAAATTGGTCCCATAATACAATTCATAGTAGAAGGTCATATTCAAATATTGTTTTACTCGTGCTTTGTTTTCTCAAAGTAAGCTTAATCTATTGAGTTTACATATCTGTTAGTGTGATCTATACAAGATGTACATTGTCTACTCTTATTGAAGTAACAGAGTTTCTCATTTCAGTTCATTATAGGGAATGGTGAAAACATGTCTGACTTCACATTTGTTGAGAATGTGACCCATGCCCATATCTGTGCAGAAGAAGCACTAGATTTTCGAATGGTGTCTGTGGCTGGAAAGGTATTGCACTTAAAATATTGTACATAGCCTTTTGTCATACTTTTAGTGATGGGATTCATAAGAAAGTATTTCTTTTTTTATCATTATGTAGGCATTTTTCATCACTAATTTTAAACCCATGAAGTTCTGGGAATTTATCTCTCTCGTTTTGGAAGGCTTGGGTTACCGAAGGTACAAGATTATAGTTCATGAACTTTTAGATGATATTGTTCTGTTGATCCCTTTCCTAAATTTTTCATGCGTTGTGTTGGCATATATTTGATGACGATATTAAAGCTAAGATAGTTTTCAGTTCTCTTCTCATTTGTCTGATAATGATGTCTTGCTACATGTTCTGACAGGCCATCAGTAAAACTTCCATTTAAGATGGTTTGGAATGTTCTCTTGTTTATGAAATGGATTGATGAAAAGTTCGGATTCAATAAATATAATCATTCCACTTGGGCTCATTATATTCAGCTAGCCTCGTGCACTAGAACCTTTAATTGTGTTGCTGCACATAAACAAATTGGGTACTCTCCTATAGTCTCGTTGGAAGTAAGTTGTTATGAAAATATATATCAGTTTCTTATTTACTCATTTCTTTTTTATTTACAGTGTATCATTGGAATATTGTCTTCTGTTTTTCTCACTTGTCTTTGTTTTCTATCCTACCCATATGAGGTCTTCGATTAATTTATATATAAGGCTGCCAATGGCAAAACCAAAAATGGTGTATTCTGTTGTTGATTTAAAATTTTAAAAGAAGTCAGGTTTTTCTGTGTCGTTGTCTTCTGGTTTAATATCTCTCTCTCTCTCTCTCTCTGTCTCACATGTATAGTGCAGGCACACACTGATACTACTTCGTTGCAGGTTGTGAACAATTACTATGTTGTGTTGATTTCAAAATACATGACTTTAGTAGGAATTGTATGTGTAATTTTTTTGGGTAAAAATCAAATTTCAAATTATTGATGAATGAAATTACAAAGGAGGAGCAAAGGCTCAATTCAAACCCATGGCAAATTAAAGCTCCTATAATATTAAGAGGAATGGGTTCAATTACTCGAAGAAATGGAGAGATCACTAAGAGCAAGTAAAAAAACCAGTTTCTCCAAAGCAGAAGAAAAGTCTGAGCGTTCGTTCTTCAAAGTATGCTTGTTCCTATCTCTCCATAAATCCCATTAAATGCGCTGATACAAGATCCTCAATCACCTTTTATTTTCTTTCTTAAAACGGTTGTCCCTAAGCCTTTTTAATAGTTGAGAGAGAATAGAGTTTAGGGTAAAGAGTGTAAAGAGGTCTGTCACCTTCCCATAAATCTTCCTAAAAGAGAGTGTTTTTGCCTGATGAACCTGATCTTGCTTGCTATGATGGATTTTAGCTTGATGGTTGTTCCCCAAGGCCTTTTGGAGAATTTGATTTAGTGGTGCAAGGCTTCCAATCTCAACTTTTCATGGAATTCTGCTTGTATTTCCTCTTCAACTTTTCTGACATCCAAAAGCCATTTTAGATCTTAGGGATCTCATGCCTTGTATCTTTGCAAATAAGACATTGTGGAGCTAGGCACTAGCTGATGTATTTGGATATGTTAGGGAAGCTAGGGTCAAGGTTGCAGCTTATATATCTTCTGCAACTTCTGTTTCATTTGAATAGTCGCACCTTTGTTTTAAGCAGTAATAGCTTATATGAGTTTTAATTTGTTCATTTCTTTTTCTTTACTGGTCAAATTTACAGGAAGGCATCACACTAACCATTAAATCATTTTCACATTTGCGCAAAGAATTGTCTCTTGCAAGTTTCACTGAATTTACTGACGAATCAAAAGCTGATAAGCTACTGGGTGGTGGAAGAGGTTCAAACCTTCTCTCATTTAATCTATTTGGCATCATTTTCTATACTAATAATAATATTGCTTAGACAACTTCAATCGTTATTTAGTTGGATTTTCTGATTTCAACTTATGGACCTTGTGTTCTCTAGATGATTGCACTTTTTTCTGCTATTCTATGTTTAACTTGAATGGTTATTGCCATTTGTTTTTTTTTATAAAAAAAAAACGCATGAACATCGCCTCATGCAAAACCCCCAGCAGCTCGACTGGGGCTGAATGAGAGCATGCTTGAAATACAATATAATTATATGCAGCAGATACACGGATAAATTGGCTCTTTTGAAGAAATATGGAATATGGCAGGAAACACCAGTTATTTCCCCTGGAGCACGTTAATAATTGTCCTGCAATGGTATGGCCTTGCATGGCCAATACTGCATGGCTCTTAGTCTCTAAATTGTAGCTTGAAACTGGGATAGATCTTGAGTACTTGGGAGAGCTTGGTTTTTTGGATCATATCACAGCCCTTGTATTTTCCTTTTGAGATGAGAAACCAACATTTTCATTCAAATAGATGAAAGAAGTAAAAAAACACCAAAAACAAGCTAGAAACAGCATAGCCGAAAGGAAATATTTACACTTAAAACAATCCTTTTACTTTGTATTTCTTATTTAACTTCAATAAATATTGTTAAACGACCCATGATTTAGGTGGGGGTCTATGTGAAATACTTGGAATTTGTTGGGTTTTGGATACATGTGCCTTAAATATAGATATTTGAAGTGTTACAGAAAAAATGATGCTCGTTGATTAAATCCAATACATCTAAAAAGTATACTACGAATATGGTAATATCTATATTGTTTTAGAGCTGACAGATTGAATCCATGATATAGAAATTGATGGGAATTAATGAAAAGATCTGGTTGTATTTCTGATAAGTTAGTCTAAGAGTTTTGGTTGAATTCGTTAGTTTGTCATTAAATGCTCTGTGAATATTGGGTTAGTCTCTAGTAGTTTGGTTTTACTGGTACTTCTTCATTTTATTAGCGAAAAGTATAGTTTCTTTGCTAAAAAATAGCCTATGGAGGAGATCTCGCTTACTGATGAATGTTTTGAAATTCATGTGTTTACAATTTTTAAATTTGTAATTGGTCTTCCTTATTGCTGCATCATTTGGTTTACCTCCGTAACATTAGCTAAATTTGTACTAGGAGAATGACATTGGACTTCTTTCTGCAGTTGCCGATATATTGTTATGGCGAGAAGAGAAGAAATCATTCACGTGTTTTCTTGCTTCGTCTCTATTGTTTTATTGGTTTTTTGTACGTGGAGGAACTTTTATATCATCAGCAGCTCAACTTCTGTTGTCTATCATTGTTGTCCTTTATGGATATGGGTTCATACCACCAAACATGTAAGTAGGCTCATGAAGTCCTACCCTGGCATTCGATGCTTAAAGCTGCAGTTCTAAATGCAATGATTATCACATTTTTTAATGACTATAGATTACTTTCTTTATTGCGGAAGTGGAAGTGAGATATTTCACATTCAAAATTCTAGGATCCTTGATTTATTGCTCTGCTGTTATTTGTTTCGTATGATTATTTGCCTTCTCGTGTACGTCCACGGGCATGATAAATCCTCCTTTTTTTCCCCCCTCGACAGAAGCTCCTTTTTATCCGTATTTCTTGTAATTCATCATTATTGCAGTTGTTGTTTGTTGAACTGATAATGAGGTTTTCCCATCAGATACGGGTTTCCTGTTCAAAAGTTATCTGTGACAGCGTTTCGGCAATCTGATTCAGTAGTCAGAGATTCAATCATGACCCTAGCATGCTTGTGGAATAGAGGTGTACATAACGCAAGATCATTGGCCCGAGGAGAGGATTGGAACTATTTTTTTAAGGTATGACTGCAACACTTGTTCTTCGTGTGTGTGGGTGCTATACTGTGTGGACATATTTGTATTTCAGGAAGTCTTGCAACAATGCAGTGATGACAACATTGATCTCACTGCATTTGTCCCAGTATTTTCTTAAACAAATCGAGAGACCCAATAGTGATGCACACAGGTGTAGGGAATTTACCCAAATTGACAAAAAATCCCAAAACTACAGAGAGACACCATGTGTATAAATATGGCAAGGCTGGTAATTAACCCCAACTTAAGACTTTAAACAACAGTAACTAAAGCCTAAAGTCTAAAATTCAGAAAGGAATCGGTTGTGGTAAAAGACTATTACCACCCGAATAATGCTAACAAACAAGAACCTAAAGAAACAGAGAAGAATGTTCAAGTGAAGCCAAGAAAAGCCAAGAACATTAAGACATCCCTACATCAAATAGTCTTTTGGGCTTAGGCTTGAAGCACGGTGAAAAAGAGAAAACAAACCTCTTTTTGTTGGTTGTGAAGCATAATGTGTGAAAAAAATGAAAAATATAGCGTTGTGGATAATGAGATAATGAAAATTAGGTTAAATGATAAGTTTAGTCTTTAAATTTCCAGGGTTGTGTCTAATAACTTCTTAAATTTTAAAAAGCATTTGAGTGGTCTCTTATAGGGTCAAAGACTTTAAAAGTGTCTAATAGATCTTTAAATTTTCAATTTTATGTCTAATAGGTCATGAGTGTATTGAACAGTTTTTAAATTTCATGAACTTGTTAGTTATTAAATTAAATTTTATGTGAAATAAAAGCATTCCTATTTTTTGTTTAATAGGCCCGTGAATTTAAAGAAATTGTTAGAAAGTCATGGACATTGAAATTAATAGTTTGGGAACCAATTAGACACTTTTAATTCAAGGATCCAGTAAACACAAAATTGAAAGTTTATGAACCTATTCAGCACTTTATAAAGTTTAGGGGCTTGATAGACACAAATTTTAACGATCTTGAAAGTTTAGAAACTAAAATTAATTTAACCAAAAAATTAGATGGTTTAGACTTCACAAGGCACTTCATGGTAGCTATCAATGCTTGAAATTGTCGTGAAGAACCCTATTTTCATGTAATAATTAGTTTGAAATGAAGTGTTTTAAGTTATAGAATTATCAAACTCATTTTGACTTCATATCTAACCACAATCAAAATGTTGCGCTTTTTAAAAGTCATTTTGATGAACTAGTAGTGTACATGCACCTCCCCTTTTTCGTTTTATAATAATTTCACACAATTAAACGTTCTATTGGTTACAGGCTGTGGCATTTCTTTATATCCTCAAACTGTTACTCTCACAATCCTTGACTATGCTGTTAGGCATGGGTAAGCAACAACTAGGTTTATTTCTGACACGAGGCGCTGCTTGTTGTTTGTGACTTTTTTTCTTTTTTTTTTCTTTTTACTTCTTCCTTGCCACTTATTTTATAGTAATTAATGGTTTTAATATTGCAGGACTAGTGTTTGCGTTCACTGCATTCTTTGTGTATGAGCAATATGAAGCTGAGATAGACGAATTCGCAAAGTTCTTTTTCATTGGCATCATGGAATTAAAGAGACTACTTGCAAGTCATTTACCAACACCATTGATGTCACTTCTCTGTTGTGACAGAGTATCACATCAAGCTGCAGTGACAAAGCGATAGAGCAGAATCCCAAAACGTACAAGGATCGGAAGATTTTTCGGGTGTAGCTCCTTTTGATCAATTCGTTAGCTTACTCGGGTGTCTACCAGAATATGTAAAGCCTTTCCGCCTAGTTGAAGCAAAGAAATGGAGGTAATCTATCATATTACAAAACCCAACCCGCATCTTATCTTCTCGCAACATTTTTCCACAATCACTATGCAATGCCTTTTTAACACTTATGTCAAAGATCAGAAGCCAAATAAAATGTTGAAATTCGATACGTAGTTTAGACGAGCAACCACCGAGATGCAGATTAATATTTGGGTGAGTCTGGTTTTTGCCTCCTTTTCTGTTAACATTACTTGTACGACTGGTAGCCAGCAGCTAGTGCAGTTCTTCAAATGTGGGGCTGCAAGTTTGTTTTCCTTGAAACCTCATATAGGCTTGAAAAAGATGATGCAAATTCCCTCCCCTTTAATGCAATGCAATTTCATAAGCCTTCTACTTTGTGGTTCACCTTAGGTGCTTGTTGATGGTATATAATGAATCATGATTTGATCTGTCTATAAATCTTTCAAGATTAAAAAGAAACTTTTATTTTTTTGTCGATTTGAACTTATATCCCGATCAGTTAACTTCATAAACGTTCAAATTGGATTTTTGATACCCTTGTTTGGTACAAGGTTTCTGGGTTGAACATATTCT

mRNA sequence

AAATTTCCGCGCCAACAACTTCTCTTTCTCTCTAGCGTCGGATTCCAACCCTAACAACCTCAATGCATTTCCCTCTCAATCTCATTCTCAGTTCTCTCTTTTTTCTCTGATCTTTCCTCCTCCGATCCCACCATTAACAAATGGAGGTCGACGATCGCTTTGTTGAATTGAGTACCAAGACTTGCGTTGTGTTGGGCGGCCGTGGATTTCTCGGCCGATCGCTGGTTCTCCGGCTCCTCAAACTCGGTAAGTGGAATGTCCGTATCGCTGATTCTGCGGAGTCGCTTGAACTCGATCCATCTGAGCATGATTCTCTCCTCTCTGACGCCCTTATCGCTGGCCGAGCTTCATATTTCCATGTCGATGTTCGCAATAAGGATCAGATCGTTGCAGCTCTCAGTGGTTCTTCCGTTGTGTTTTATATGGACTATGAAGATTCACATTCAAATGATATTTATCTCTCATATATGTTTATAGTTCAAGGTGCCAAAAATGTCATTAATGCTTGTCGAGACTGCAAAGTAAGAAGGCTAATATTCAACAGTTCAGCTGATGTAATTTTTGATGGTTCGCGTGATATACGTAATGGAGAGGAATCATTAACATACCCATGGAAATTTGAGGACATGTTGAGTGACCTAAAGGCTCAAGCTGAAGCACTCATTCTGTTTGCCAACGATATAGATGGACTTCTAACATGTGCACTTCGCCCTAGCAATGTTTTTGGGCCAGAAGACACTCGACTTGTTCCTTTCATGGTGCATCAGGCAAAATCTGGTTTGGCAAAGTTCATTATAGGGAATGGTGAAAACATGTCTGACTTCACATTTGTTGAGAATGTGACCCATGCCCATATCTGTGCAGAAGAAGCACTAGATTTTCGAATGGTGTCTGTGGCTGGAAAGGCATTTTTCATCACTAATTTTAAACCCATGAAGTTCTGGGAATTTATCTCTCTCGTTTTGGAAGGCTTGGGTTACCGAAGGCCATCAGTAAAACTTCCATTTAAGATGGTTTGGAATGTTCTCTTGTTTATGAAATGGATTGATGAAAAGTTCGGATTCAATAAATATAATCATTCCACTTGGGCTCATTATATTCAGCTAGCCTCGTGCACTAGAACCTTTAATTGTGTTGCTGCACATAAACAAATTGGGTACTCTCCTATAGTCTCGTTGGAAGAAGGCATCACACTAACCATTAAATCATTTTCACATTTGCGCAAAGAATTGTCTCTTGCAAGTTTCACTGAATTTACTGACGAATCAAAAGCTGATAAGCTACTGGGTGGTGGAAGAGCGTTTCGGCAATCTGATTCAGTAGTCAGAGATTCAATCATGACCCTAGCATGCTTGTGGAATAGAGGTGTACATAACGCAAGATCATTGGCCCGAGGAGAGGATTGGAACTATTTTTTTAAGGAAGTCTTGCAACAATGCAGTGATGACAACATTGATCTCACTGCATTTGTCCCAGCTGTGGCATTTCTTTATATCCTCAAACTGTTACTCTCACAATCCTTGACTATGCTGTTAGGCATGGGTAAGCAACAACTAGGACTAGTGTTTGCGTTCACTGCATTCTTTGTGTATGAGCAATATGAAGCTGAGATAGACGAATTCGCAAAGTTCTTTTTCATTGGCATCATGGAATTAAAGAGACTACTTGCAAGTCATTTACCAACACCATTGATGTCACTTCTCTGTTGTGACAGAGTATCACATCAAGCTGCAGTGACAAAGCGATAGAGCAGAATCCCAAAACGTACAAGGATCGGAAGATTTTTCGGGTGTAGCTCCTTTTGATCAATTCGTTAGCTTACTCGGGTGTCTACCAGAATATGTAAAGCCTTTCCGCCTAGTTGAAGCAAAGAAATGGAGGTAATCTATCATATTACAAAACCCAACCCGCATCTTATCTTCTCGCAACATTTTTCCACAATCACTATGCAATGCCTTTTTAACACTTATGTCAAAGATCAGAAGCCAAATAAAATGTTGAAATTCGATACGTAGTTTAGACGAGCAACCACCGAGATGCAGATTAATATTTGGGTGAGTCTGGTTTTTGCCTCCTTTTCTGTTAACATTACTTGTACGACTGGTAGCCAGCAGCTAGTGCAGTTCTTCAAATGTGGGGCTGCAAGTTTGTTTTCCTTGAAACCTCATATAGGCTTGAAAAAGATGATGCAAATTCCCTCCCCTTTAATGCAATGCAATTTCATAAGCCTTCTACTTTGTGGTTCACCTTAGGTGCTTGTTGATGGTATATAATGAATCATGATTTGATCTGTCTATAAATCTTTCAAGATTAAAAAGAAACTTTTATTTTTTTGTCGATTTGAACTTATATCCCGATCAGTTAACTTCATAAACGTTCAAATTGGATTTTTGATACCCTTGTTTGGTACAAGGTTTCTGGGTTGAACATATTCT

Coding sequence (CDS)

ATGGAGGTCGACGATCGCTTTGTTGAATTGAGTACCAAGACTTGCGTTGTGTTGGGCGGCCGTGGATTTCTCGGCCGATCGCTGGTTCTCCGGCTCCTCAAACTCGGTAAGTGGAATGTCCGTATCGCTGATTCTGCGGAGTCGCTTGAACTCGATCCATCTGAGCATGATTCTCTCCTCTCTGACGCCCTTATCGCTGGCCGAGCTTCATATTTCCATGTCGATGTTCGCAATAAGGATCAGATCGTTGCAGCTCTCAGTGGTTCTTCCGTTGTGTTTTATATGGACTATGAAGATTCACATTCAAATGATATTTATCTCTCATATATGTTTATAGTTCAAGGTGCCAAAAATGTCATTAATGCTTGTCGAGACTGCAAAGTAAGAAGGCTAATATTCAACAGTTCAGCTGATGTAATTTTTGATGGTTCGCGTGATATACGTAATGGAGAGGAATCATTAACATACCCATGGAAATTTGAGGACATGTTGAGTGACCTAAAGGCTCAAGCTGAAGCACTCATTCTGTTTGCCAACGATATAGATGGACTTCTAACATGTGCACTTCGCCCTAGCAATGTTTTTGGGCCAGAAGACACTCGACTTGTTCCTTTCATGGTGCATCAGGCAAAATCTGGTTTGGCAAAGTTCATTATAGGGAATGGTGAAAACATGTCTGACTTCACATTTGTTGAGAATGTGACCCATGCCCATATCTGTGCAGAAGAAGCACTAGATTTTCGAATGGTGTCTGTGGCTGGAAAGGCATTTTTCATCACTAATTTTAAACCCATGAAGTTCTGGGAATTTATCTCTCTCGTTTTGGAAGGCTTGGGTTACCGAAGGCCATCAGTAAAACTTCCATTTAAGATGGTTTGGAATGTTCTCTTGTTTATGAAATGGATTGATGAAAAGTTCGGATTCAATAAATATAATCATTCCACTTGGGCTCATTATATTCAGCTAGCCTCGTGCACTAGAACCTTTAATTGTGTTGCTGCACATAAACAAATTGGGTACTCTCCTATAGTCTCGTTGGAAGAAGGCATCACACTAACCATTAAATCATTTTCACATTTGCGCAAAGAATTGTCTCTTGCAAGTTTCACTGAATTTACTGACGAATCAAAAGCTGATAAGCTACTGGGTGGTGGAAGAGCGTTTCGGCAATCTGATTCAGTAGTCAGAGATTCAATCATGACCCTAGCATGCTTGTGGAATAGAGGTGTACATAACGCAAGATCATTGGCCCGAGGAGAGGATTGGAACTATTTTTTTAAGGAAGTCTTGCAACAATGCAGTGATGACAACATTGATCTCACTGCATTTGTCCCAGCTGTGGCATTTCTTTATATCCTCAAACTGTTACTCTCACAATCCTTGACTATGCTGTTAGGCATGGGTAAGCAACAACTAGGACTAGTGTTTGCGTTCACTGCATTCTTTGTGTATGAGCAATATGAAGCTGAGATAGACGAATTCGCAAAGTTCTTTTTCATTGGCATCATGGAATTAAAGAGACTACTTGCAAGTCATTTACCAACACCATTGATGTCACTTCTCTGTTGTGACAGAGTATCACATCAAGCTGCAGTGACAAAGCGATAG

Protein sequence

MEVDDRFVELSTKTCVVLGGRGFLGRSLVLRLLKLGKWNVRIADSAESLELDPSEHDSLLSDALIAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINACRDCKVRRLIFNSSADVIFDGSRDIRNGEESLTYPWKFEDMLSDLKAQAEALILFANDIDGLLTCALRPSNVFGPEDTRLVPFMVHQAKSGLAKFIIGNGENMSDFTFVENVTHAHICAEEALDFRMVSVAGKAFFITNFKPMKFWEFISLVLEGLGYRRPSVKLPFKMVWNVLLFMKWIDEKFGFNKYNHSTWAHYIQLASCTRTFNCVAAHKQIGYSPIVSLEEGITLTIKSFSHLRKELSLASFTEFTDESKADKLLGGGRAFRQSDSVVRDSIMTLACLWNRGVHNARSLARGEDWNYFFKEVLQQCSDDNIDLTAFVPAVAFLYILKLLLSQSLTMLLGMGKQQLGLVFAFTAFFVYEQYEAEIDEFAKFFFIGIMELKRLLASHLPTPLMSLLCCDRVSHQAAVTKR
BLAST of Lsi05G013430 vs. Swiss-Prot
Match: HSDD3_ARATH (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 3 OS=Arabidopsis thaliana GN=3BETAHSD/D3 PE=2 SV=2)

HSP 1 Score: 431.4 bits (1108), Expect = 1.4e-119
Identity = 225/386 (58.29%), Postives = 279/386 (72.28%), Query Frame = 1

Query: 1   MEVDDRFVELSTKTCVVLGGRGFLGRSLVLRLLKLGKWNVRIADSAESLELDPSEHDSLL 60
           M+ D    +   KTCVVLGGRGF+GRSLV RLL+LG W VR+ADS  +L LD  E DSLL
Sbjct: 1   MDEDSVHGDSHLKTCVVLGGRGFIGRSLVSRLLRLGNWTVRVADSGHTLHLD--ESDSLL 60

Query: 61  SDALIAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVI 120
            DAL +GRASY  VDVR+K QIV    GS VVFYM   D  S+D +  Y  IVQG +NVI
Sbjct: 61  EDALSSGRASYHCVDVRDKPQIVKVTEGSYVVFYMGATDLRSHDYFDCYKVIVQGTRNVI 120

Query: 121 NACRDCKVRRLIFNSSADVIFDGSRDIRNGEESLTYPWKFEDMLSDLKAQAEALILFAND 180
           +ACR+  VR+LI+NS+ADV+FDGS+ IR+G+ESL  P KF+ ML+D KAQAEALI  AN+
Sbjct: 121 SACRESGVRKLIYNSTADVVFDGSQPIRDGDESLRRPLKFQSMLTDFKAQAEALIKLANN 180

Query: 181 IDGLLTCALRPSNVFGPEDTRLVPFMVHQAKSGLAKFIIGNGENMSDFTFVENVTHAHIC 240
            DGLLTCALR S VFGP DT  VPF+V+ AKSG AKFI+G+GEN+SDFT+ ENV+HAHIC
Sbjct: 181 RDGLLTCALRSSIVFGPGDTEFVPFLVNLAKSGYAKFILGSGENISDFTYSENVSHAHIC 240

Query: 241 AEEALDFRMVSVAGKAFFITNFKPMKFWEFISLVLEGLGYRRPSVKLPFKMVWNVLLFMK 300
           A +ALD +M  VAGK FFITN KP++FW+F+S ++EGLGY RPS+KLP ++V  V   +K
Sbjct: 241 AVKALDSQMEFVAGKEFFITNLKPVRFWDFVSHIVEGLGYPRPSIKLPVRLVLYVFSLLK 300

Query: 301 WIDEKFGFNKYNHSTWAHYIQLASCTRTFNCVAAHKQIGYSPIVSLEEGITLTIKSFSHL 360
           W  EK G    N+ T   Y  LAS TRTFNC AA K +GY+P+V+LE+GI  T++ FS  
Sbjct: 301 WTHEKEGLGS-NYDTAHQYALLASSTRTFNCNAAKKHLGYTPVVTLEDGIASTLQWFSR- 360

Query: 361 RKELSLASFTEFTDESKADKLLGGGR 387
                L    +   +S AD+LLG G+
Sbjct: 361 ----DLEKSDDTIIQSTADQLLGCGK 378

BLAST of Lsi05G013430 vs. Swiss-Prot
Match: HSDD2_ARATH (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 OS=Arabidopsis thaliana GN=3BETAHSD/D2 PE=2 SV=2)

HSP 1 Score: 327.8 bits (839), Expect = 2.2e-88
Identity = 174/376 (46.28%), Postives = 240/376 (63.83%), Query Frame = 1

Query: 15  CVVLGGRGFLGRSLVLRLLKLGKWNVRIADSAESLELDPSEHDSLLSDALIAGRASYFHV 74
           CVV GGRGF  R LV  L++   + VRIAD A ++ LDP E + +L + L +GR  Y   
Sbjct: 12  CVVTGGRGFAARHLVEMLVRYEMFCVRIADLAPAIMLDPQEGNGVLDEGLRSGRVQYISA 71

Query: 75  DVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINACRDCKVRRLIFN 134
           D+R+K Q+V A  G+ VVF+M   DS  N+  L Y   VQG +NVI+AC D  V+RLI+ 
Sbjct: 72  DLRDKSQVVKAFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTQNVIDACVDVGVKRLIYT 131

Query: 135 SSADVIFDGSRDIRNGEESLTYPWKFEDMLSDLKAQAEALILFANDIDGLLTCALRPSNV 194
           SS  V+FDG   I NG ES+ YP K  D  S  KA+ E LI+ AN  +GLLTC +RPS++
Sbjct: 132 SSPSVVFDGVHGILNGTESMAYPIKHNDSYSATKAEGEELIMKANGRNGLLTCCIRPSSI 191

Query: 195 FGPEDTRLVPFMVHQAKSGLAKFIIGNGENMSDFTFVENVTHAHICAEEALDFR---MVS 254
           FGP D  LVP +V  A++G +KFIIG+G N+ DFT+VENV HAH+CAE AL         
Sbjct: 192 FGPGDRLLVPSLVAAARAGKSKFIIGDGNNLYDFTYVENVAHAHVCAERALASGGDVSTK 251

Query: 255 VAGKAFFITNFKPMKFWEFISLVLEGLGYRRPSVKLPFKMVWNVLLFMKWIDEKFG-FNK 314
            AG+A+FITN +P+KFWEF+S +L+GLGY RPS+K+P  ++  +   ++   +  G +  
Sbjct: 252 AAGQAYFITNMEPIKFWEFMSQLLDGLGYERPSIKIPAFIMMPIAHLVELTYKVLGPYGM 311

Query: 315 YNHSTWAHYIQLASCTRTFNCVAAHKQIGYSPIVSLEEGITLTIKSFSHLRKELSLASFT 374
                    ++L SC+RTF+   A  ++GY+P+V L+EGI  TI SFSH    L+  S +
Sbjct: 312 TVPQLTPSRVRLLSCSRTFDSTKAKDRLGYAPVVPLQEGIRRTIDSFSH----LTAGSQS 371

Query: 375 EFTDESKADKLLGGGR 387
           +    SKA ++LGGG+
Sbjct: 372 KREGPSKASRILGGGK 383

BLAST of Lsi05G013430 vs. Swiss-Prot
Match: HSDD1_ARATH (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 OS=Arabidopsis thaliana GN=3BETAHSD/D1 PE=2 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 1.0e-85
Identity = 166/353 (47.03%), Postives = 224/353 (63.46%), Query Frame = 1

Query: 15  CVVLGGRGFLGRSLVLRLLKLGKWNVRIADSAESLELDPSEHDSLLSDALIAGRASYFHV 74
           CVV GGRGF  R LV  L++   ++VRIAD A ++ L+P E   +L +A+ +GR  Y   
Sbjct: 12  CVVTGGRGFAARHLVEMLVRYQMFHVRIADLAPAIVLNPHEETGILGEAIRSGRVQYVSA 71

Query: 75  DVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINACRDCKVRRLIFN 134
           D+RNK Q+V    G+ VVF+M   DS  N+  L Y   VQG  NVI+AC +  V+RLI+ 
Sbjct: 72  DLRNKTQVVKGFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTTNVIDACIEVGVKRLIYT 131

Query: 135 SSADVIFDGSRDIRNGEESLTYPWKFEDMLSDLKAQAEALILFANDIDGLLTCALRPSNV 194
           SS  V+FDG     N +ESL YP K  D  S  KA+ EALIL AN   GLLTC +RPS++
Sbjct: 132 SSPSVVFDGVHGTLNADESLPYPPKHNDSYSATKAEGEALILKANGRSGLLTCCIRPSSI 191

Query: 195 FGPEDTRLVPFMVHQAKSGLAKFIIGNGENMSDFTFVENVTHAHICAEEALDFR---MVS 254
           FGP D  +VP +V  A++G +KFIIG+G N  DFT+VENV HAH+CAE AL         
Sbjct: 192 FGPGDKLMVPSLVTAARAGKSKFIIGDGSNFYDFTYVENVVHAHVCAERALASGGEVCAK 251

Query: 255 VAGKAFFITNFKPMKFWEFISLVLEGLGYRRPSVKLPFKMVWNVLLFMKWIDEKFG-FNK 314
            AG+A+FITN +P+KFWEF+S +LEGLGY RPS+K+P  ++  +   ++   +  G +  
Sbjct: 252 AAGQAYFITNMEPIKFWEFMSQLLEGLGYERPSIKIPASLMMPIAYLVELAYKLLGPYGM 311

Query: 315 YNHSTWAHYIQLASCTRTFNCVAAHKQIGYSPIVSLEEGITLTIKSFSHLRKE 364
                    ++L SC RTF+   A  ++GYSP+V L+EGI  TI SFSHL+ +
Sbjct: 312 KVPVLTPSRVRLLSCNRTFDSSKAKDRLGYSPVVPLQEGIKRTIDSFSHLKAQ 364

BLAST of Lsi05G013430 vs. Swiss-Prot
Match: NSDHL_RAT (Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating OS=Rattus norvegicus GN=Nsdhl PE=2 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 6.3e-59
Identity = 132/353 (37.39%), Postives = 195/353 (55.24%), Query Frame = 1

Query: 13  KTCVVLGGRGFLGRSLVLRLLKLGKWNVRIADSAESLELDPSEHDSLLSDALIAGRASYF 72
           K C V+GG GFLG+ +V +LL  G + V + D  +  + +P              R  +F
Sbjct: 27  KKCTVIGGSGFLGQHMVEQLLSRG-YAVNVFDVRQGFD-NP--------------RVQFF 86

Query: 73  HVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINACRDCKVRRLI 132
             D+ N+  +  AL G S VF+     S+SN+  L Y     G K VI  C++  V++LI
Sbjct: 87  IGDLCNQQDLYPALKGVSTVFHCASPPSNSNNKELFYRVNSTGTKTVIETCKEAGVQKLI 146

Query: 133 FNSSADVIFDGSRDIRNGEESLTYPWKFEDMLSDLKAQAEALILFANDID-GLLTCALRP 192
             SSA V+F+G  DI+NG E L Y  K  D  ++ K   E  +L AND     LT A+RP
Sbjct: 147 LTSSASVVFEGV-DIKNGTEDLPYAMKPIDYYTETKILQERAVLDANDPKKNFLTAAIRP 206

Query: 193 SNVFGPEDTRLVPFMVHQAKSGLAKFIIGNGENMSDFTFVENVTHAHICAEEALDFRMVS 252
             +FGP D +LVP ++  A+ G  KF+IGNG+N+ DFTFVENV H HI A E L  R   
Sbjct: 207 HGIFGPRDPQLVPVLIDAARKGKMKFMIGNGKNLVDFTFVENVVHGHILAAEHLS-RDAG 266

Query: 253 VAGKAFFITNFKPMKFWEFISLVLEGLGYRRPSVKLPFKMVWNVLLFMKWIDEKFGFNKY 312
           + GKAF ITN +P+ FW F+S +L GL Y  P   +P+++ + +   +  +         
Sbjct: 267 LGGKAFHITNDEPIPFWTFLSRILTGLNYEAPKYHIPYRVAYYLAFLLSLLVMVLSPLIQ 326

Query: 313 NHSTWAHY-IQLASCTRTFNCVAAHKQIGYSPIVSLEEGITLTIKSFSHLRKE 364
             +T+  + + LA     ++C  A K IGY P+V++++ +  T++SF HLRK+
Sbjct: 327 IQTTFTPFRVALAGTFHYYSCEKAKKLIGYRPLVTMDDAVERTVQSFHHLRKD 361

BLAST of Lsi05G013430 vs. Swiss-Prot
Match: NSDHL_MOUSE (Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating OS=Mus musculus GN=Nsdhl PE=1 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 4.1e-58
Identity = 133/353 (37.68%), Postives = 192/353 (54.39%), Query Frame = 1

Query: 13  KTCVVLGGRGFLGRSLVLRLLKLGKWNVRIADSAESLELDPSEHDSLLSDALIAGRASYF 72
           K C V+GG GFLG+ +V +LL+ G + V + D  +  + +P              R  +F
Sbjct: 27  KKCTVIGGSGFLGQHMVEQLLERG-YTVNVFDIHQGFD-NP--------------RVQFF 86

Query: 73  HVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINACRDCKVRRLI 132
             D+ N+  +  AL G S VF+      +SN+  L Y     G K VI  CR+  V++LI
Sbjct: 87  IGDLCNQQDLYPALKGVSTVFHCASPPPYSNNKELFYRVNFIGTKTVIETCREAGVQKLI 146

Query: 133 FNSSADVIFDGSRDIRNGEESLTYPWKFEDMLSDLKAQAEALILFANDID-GLLTCALRP 192
             SSA V+F+G  DI+NG E L Y  K  D  ++ K   E  +L AND     LT A+RP
Sbjct: 147 LTSSASVVFEGV-DIKNGTEDLPYAMKPIDYYTETKILQERAVLDANDPKKNFLTAAIRP 206

Query: 193 SNVFGPEDTRLVPFMVHQAKSGLAKFIIGNGENMSDFTFVENVTHAHICAEEALDFRMVS 252
             +FGP D +LVP ++  A+ G  KF+IGNGEN+ DFTFVENV H HI A E L  +  +
Sbjct: 207 HGIFGPRDPQLVPILIDAARKGKMKFMIGNGENLVDFTFVENVVHGHILAAEHLS-QDAA 266

Query: 253 VAGKAFFITNFKPMKFWEFISLVLEGLGYRRPSVKLPFKMVWNVLLFMKWIDEKFGFNKY 312
           + GKAF ITN +P+ FW F+S +L GL Y  P   +P+ M + +   +  +         
Sbjct: 267 LGGKAFHITNDEPIPFWTFLSRILTGLNYEAPKYHIPYWMAYYLAFLLSLLVMVVSPLIQ 326

Query: 313 NHSTWAHY-IQLASCTRTFNCVAAHKQIGYSPIVSLEEGITLTIKSFSHLRKE 364
              T+    + LA     ++C  A K  GY P+V+++E +  T++SF HLRK+
Sbjct: 327 IQPTFTPIRVALAGTFHYYSCEKAKKLFGYRPLVTMDEAVERTVQSFHHLRKD 361

BLAST of Lsi05G013430 vs. TrEMBL
Match: A0A0A0L5K0_CUCSA (Reticulon-like protein OS=Cucumis sativus GN=Csa_3G174040 PE=3 SV=1)

HSP 1 Score: 754.2 bits (1946), Expect = 1.1e-214
Identity = 369/386 (95.60%), Postives = 379/386 (98.19%), Query Frame = 1

Query: 1   MEVDDRFVELSTKTCVVLGGRGFLGRSLVLRLLKLGKWNVRIADSAESLELDPSEHDSLL 60
           MEVDDRF+EL+TKTCVVLGGRGFLGRSLVLRLLKL KWNVRIADSA SLELDPSEHDSLL
Sbjct: 1   MEVDDRFLELNTKTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSANSLELDPSEHDSLL 60

Query: 61  SDALIAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVI 120
           SDALIAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVI
Sbjct: 61  SDALIAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVI 120

Query: 121 NACRDCKVRRLIFNSSADVIFDGSRDIRNGEESLTYPWKFEDMLSDLKAQAEALILFAND 180
           +ACRDCKVRRLIFNSSADVIFDGSRDI NGEESLTYPWKFEDMLSDLKAQAEALILFAND
Sbjct: 121 SACRDCKVRRLIFNSSADVIFDGSRDILNGEESLTYPWKFEDMLSDLKAQAEALILFAND 180

Query: 181 IDGLLTCALRPSNVFGPEDTRLVPFMVHQAKSGLAKFIIGNGENMSDFTFVENVTHAHIC 240
           IDGLLTCALRPSNVFGPEDTRLVPF+VHQAKSG AKFIIGNGENMSDFTFVENVTHAHIC
Sbjct: 181 IDGLLTCALRPSNVFGPEDTRLVPFIVHQAKSGFAKFIIGNGENMSDFTFVENVTHAHIC 240

Query: 241 AEEALDFRMVSVAGKAFFITNFKPMKFWEFISLVLEGLGYRRPSVKLPFKMVWNVLLFMK 300
           AEEALDFRMVSVAGKAFFITNFKPMKFWEFISL+L GLGYRRPSVKLP K+VWNVLLFMK
Sbjct: 241 AEEALDFRMVSVAGKAFFITNFKPMKFWEFISLILRGLGYRRPSVKLPSKVVWNVLLFMK 300

Query: 301 WIDEKFGFNKYNHSTWAHYIQLASCTRTFNCVAAHKQIGYSPIVSLEEGITLTIKSFSHL 360
           WIDEKFGFNKYNHSTWAHYIQLASCTRTF+C AAHKQ+GYSP+VSLEEGITLTIKSFSHL
Sbjct: 301 WIDEKFGFNKYNHSTWAHYIQLASCTRTFSCAAAHKQLGYSPVVSLEEGITLTIKSFSHL 360

Query: 361 RKELSLASFTEFTDESKADKLLGGGR 387
           RKELSLASFTEFT+ESKADKLLGGGR
Sbjct: 361 RKELSLASFTEFTEESKADKLLGGGR 386

BLAST of Lsi05G013430 vs. TrEMBL
Match: A5BT18_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_007871 PE=3 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 9.2e-158
Identity = 301/532 (56.58%), Postives = 382/532 (71.80%), Query Frame = 1

Query: 8   VELSTKTCVVLGGRGFLGRSLVLRLLKLGKWNVRIADSAESLELDPSEHDSLLSDALIAG 67
           ++ + KTCVV GGRGF+GR LVLRLLKLGKW VR+ADSA+SL+LDPSE  S+LS+A+ +G
Sbjct: 3   IDENLKTCVVFGGRGFIGRFLVLRLLKLGKWIVRVADSAQSLQLDPSEDRSVLSEAISSG 62

Query: 68  RASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINACRDCK 127
           RAS   VDVR+K Q+  A+ G+SVVFYMD   +++ND YL YM IVQG +NVINAC++CK
Sbjct: 63  RASCCAVDVRDKAQVRKAIEGASVVFYMDPTITYTNDFYLCYMIIVQGVRNVINACQECK 122

Query: 128 VRRLIFNSSADVIFDGSRDIRNGEESLTYPWKFEDMLSDLKAQAEALILFANDIDGLLTC 187
           V+RLI+NSSADV+FDGS DI NG+ESL  PWKFEDML+D+KAQAE L+L AN+IDGL+TC
Sbjct: 123 VKRLIYNSSADVVFDGSHDIYNGDESLPCPWKFEDMLTDIKAQAEGLVLIANNIDGLVTC 182

Query: 188 ALRPSNVFGPEDTRLVPFMVHQAKSGLAKFIIGNGENMSDFTFVENVTHAHICAEEALDF 247
           ALRP NVFGP D +LVPF+V++AKSG AKF+IG+GENMSDFT+VENV HAH+CAEEAL  
Sbjct: 183 ALRPCNVFGPGDKQLVPFLVNKAKSGHAKFVIGSGENMSDFTYVENVAHAHVCAEEALVS 242

Query: 248 RMVSVAGKAFFITNFKPMKFWEFISLVLEGLGYRRPSVKLPFKMVWNVLLFMKWIDEKFG 307
           RMVSVAGK FFITN +P+KFWEF+SL+LEGLGY+RP  KLP  MV  VL  ++W+ +K  
Sbjct: 243 RMVSVAGKVFFITNLEPVKFWEFVSLILEGLGYQRPLFKLPAGMVLYVLSLVEWMRDKLD 302

Query: 308 FNKYNHSTWAHY-IQLASCTRTFNCVAAHKQIGYSPIVSLEEGITLTIKSFSHLRKELSL 367
           F + NH   A Y +Q+AS TRTFNC AA K IGYSP+VSLEEG+TLT +SFS L ++ S+
Sbjct: 303 F-RSNHPVSAQYVVQIASRTRTFNCSAAQKHIGYSPVVSLEEGVTLTTESFSQLSQDSSV 362

Query: 368 ASFTEFTDESKADKLLGGGR--------AFRQSDSVVRDSI-------MTLACL-WNRGV 427
            +  +F ++SK DKLLGGG+        A      ++  +I       ++L+C   +  V
Sbjct: 363 MALRDFDEQSKVDKLLGGGKAKLLLLVTAILSGQGILPTNIFGFSIQRISLSCFEISXTV 422

Query: 428 HNARSLARGEDWNYFFKEVLQQCSDDNIDLTAFVPAVAFLYILKLLLSQSLTMLLGMGKQ 487
            N         WN +   +         D   F      LY LKL+LSQ LT+++G    
Sbjct: 423 VNDLITNMANLWNNWIHMIRLLAGGG--DWNTFFKVAIPLYFLKLILSQCLTLVVG---- 482

Query: 488 QLGLVFAFTAFFVYEQYEAEIDEFAKFFFIGIMELKRLLASHLPTPLMSLLC 523
            + LV AFT+FFVYEQYE E+D  A+  F  I + KRLL  +LP PL+S LC
Sbjct: 483 -VALVLAFTSFFVYEQYEEEMDGLAEVLFDSIKKSKRLLMRNLPAPLVSFLC 526

BLAST of Lsi05G013430 vs. TrEMBL
Match: M5XMU8_PRUPE (Reticulon-like protein OS=Prunus persica GN=PRUPE_ppa003251mg PE=3 SV=1)

HSP 1 Score: 548.5 bits (1412), Expect = 8.9e-153
Identity = 271/387 (70.03%), Postives = 315/387 (81.40%), Query Frame = 1

Query: 1   MEVDDRFVELSTK--TCVVLGGRGFLGRSLVLRLLKLGKWNVRIADSAESLELDPSEHDS 60
           M +DDR  EL+ K  TCVV GGRGFLGRSLVLRLLKLGKW VR+ADS +SL LDPSE DS
Sbjct: 1   MAMDDRLAELNPKPRTCVVFGGRGFLGRSLVLRLLKLGKWIVRVADSTQSLHLDPSERDS 60

Query: 61  LLSDALIAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKN 120
           LLS AL +G AS   VDVR+K QI+ A+ GSSVVFY D  D  ++D Y  YM +VQGAKN
Sbjct: 61  LLSQALSSGIASLHGVDVRDKSQIITAIEGSSVVFYFDGADLRTDDFYHCYMIVVQGAKN 120

Query: 121 VINACRDCKVRRLIFNSSADVIFDGSRDIRNGEESLTYPWKFEDMLSDLKAQAEALILFA 180
           VI+ACR+CKVRRLI+NSSADV+FDGS DI NG+ESL YPWKF+DMLSD KAQAEAL+L +
Sbjct: 121 VISACRECKVRRLIYNSSADVVFDGSHDINNGDESLPYPWKFDDMLSDFKAQAEALVLRS 180

Query: 181 NDIDGLLTCALRPSNVFGPEDTRLVPFMVHQAKSGLAKFIIGNGENMSDFTFVENVTHAH 240
           NDIDGLLTCALRPSNVFG  D+ LVPF+V+ AKSG  KFIIG+GEN SDFT+ ENVTHAH
Sbjct: 181 NDIDGLLTCALRPSNVFGHGDSHLVPFLVNLAKSGWGKFIIGSGENRSDFTYAENVTHAH 240

Query: 241 ICAEEALDFRMVSVAGKAFFITNFKPMKFWEFISLVLEGLGYRRPSVKLPFKMVWNVLLF 300
           ICAEEALD RMVS AGKAFFITN +P  FWEF++L+L+GLGY+RP VK+P KM   +LLF
Sbjct: 241 ICAEEALDNRMVSAAGKAFFITNLEPANFWEFVTLILDGLGYQRPLVKVPAKMARYILLF 300

Query: 301 MKWIDEKFGFNKYNHSTWAHYIQLASCTRTFNCVAAHKQIGYSPIVSLEEGITLTIKSFS 360
            K + EK+G  K+NHS  AHYIQLAS TRTF+C AA  QIGYSP+VSLEEG+TLTI SFS
Sbjct: 301 FKLMTEKYGLIKHNHSMSAHYIQLASHTRTFDCTAARNQIGYSPVVSLEEGVTLTIGSFS 360

Query: 361 HLRKELSLASFTEFTDESKADKLLGGG 386
           HL K  S  S+++F ++SK +KLLG G
Sbjct: 361 HLAKYSSFTSYSDFNEQSKVEKLLGSG 387

BLAST of Lsi05G013430 vs. TrEMBL
Match: B9SU06_RICCO (Reticulon-like protein OS=Ricinus communis GN=RCOM_0454370 PE=3 SV=1)

HSP 1 Score: 522.3 bits (1344), Expect = 6.9e-145
Identity = 255/387 (65.89%), Postives = 313/387 (80.88%), Query Frame = 1

Query: 1   MEVDDRFVELSTKTCVVLGGRGFLGRSLVLRLLKLGKWNVRIADSAESLELDPSEH-DSL 60
           M ++  F EL+ +TC V+GGRGF+GRSLV  LLKLG W VR+ DSA+SL+LD ++  DS 
Sbjct: 1   MAIEVPFGELNPRTCTVVGGRGFIGRSLVFNLLKLGNWIVRVVDSAQSLQLDLTDSLDSS 60

Query: 61  LSDALIAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNV 120
           L+DA+ +GRASY ++DV +   I  A+ GSSVVFYM+  D  ++D Y  Y  +VQGAKNV
Sbjct: 61  LNDAISSGRASYHYIDVIDISVIAKAIEGSSVVFYMEATDLWTHDFYYCYRNVVQGAKNV 120

Query: 121 INACRDCKVRRLIFNSSADVIFDGSRDIRNGEESLTYPWKFEDMLSDLKAQAEALILFAN 180
           I+ACR CKVR+LI+NSSADVIFDGS DI NG+ESL   W+FEDML+DLKA AEAL+LFAN
Sbjct: 121 IDACRQCKVRKLIYNSSADVIFDGSHDICNGDESLPCHWRFEDMLNDLKAHAEALVLFAN 180

Query: 181 DIDGLLTCALRPSNVFGPEDTRLVPFMVHQAKSGLAKFIIGNGENMSDFTFVENVTHAHI 240
           +IDGLLTCALRPSNVFGP DT+L+PF++  AKSG AKFIIG+GENMSDFT+VENV HAHI
Sbjct: 181 NIDGLLTCALRPSNVFGPGDTQLLPFLISLAKSGCAKFIIGSGENMSDFTYVENVAHAHI 240

Query: 241 CAEEALDFRMVSVAGKAFFITNFKPMKFWEFISLVLEGLGYRRPSVKLPFKMVWNVLLFM 300
           CA EALD RMV VAGKAFFITN +PMKFWEF SL+LE L Y+RP +KLP +MVW VLLF+
Sbjct: 241 CAAEALDSRMVCVAGKAFFITNLEPMKFWEFASLILEALSYQRPLIKLPAQMVWYVLLFI 300

Query: 301 KWIDEKFGFNKYNHSTWAHYIQLASCTRTFNCVAAHKQIGYSPIVSLEEGITLTIKSFSH 360
           KW+ EK GF+KYNHS  A++ +LAS TRTFNC+AA K IGYSP+VSL++GI LTI+++S+
Sbjct: 301 KWMHEKLGFSKYNHSMSAYFFRLASHTRTFNCIAAQKYIGYSPVVSLDDGIALTIEAYSN 360

Query: 361 LRKELSLASFTEFTDESKADKLLGGGR 387
           L K+ S      F +ESK DKLLGGG+
Sbjct: 361 LVKDPSFMRCPNFEEESKVDKLLGGGK 387

BLAST of Lsi05G013430 vs. TrEMBL
Match: A0A067DQ35_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g007866mg PE=3 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 4.5e-144
Identity = 251/381 (65.88%), Postives = 310/381 (81.36%), Query Frame = 1

Query: 7   FVELSTKTCVVLGGRGFLGRSLVLRLLKLGKWNVRIADSAESLELDPSEHDSLLSDALIA 66
           F E   +TCVVL GRGF+GRSLVLRLL+LGK  VR+ DS +SL+LDPSE +SLL D+L +
Sbjct: 3   FDEAIPRTCVVLNGRGFVGRSLVLRLLELGKCIVRVTDSTQSLQLDPSESNSLLPDSLSS 62

Query: 67  GRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINACRDC 126
           GRA Y  VDVR+  QI   L G+S VFY+D  D +++D Y  YM IVQGAKNV+ ACR+C
Sbjct: 63  GRAEYHQVDVRDISQIKKVLEGASTVFYVDATDLNTDDFYNCYMIIVQGAKNVVTACREC 122

Query: 127 KVRRLIFNSSADVIFDGSRDIRNGEESLTYPWKFEDMLSDLKAQAEALILFANDIDGLLT 186
           KVRRL++NS+ADV+FDGS DI NG+E+LT  WKF+D++ DLKAQAEAL+LFAN+IDGLLT
Sbjct: 123 KVRRLVYNSTADVVFDGSHDIHNGDETLTCCWKFQDLMCDLKAQAEALVLFANNIDGLLT 182

Query: 187 CALRPSNVFGPEDTRLVPFMVHQAKSGLAKFIIGNGENMSDFTFVENVTHAHICAEEALD 246
           CALRPSNVFGP DT+LVP +V+ AK G  KFIIG+GENMSDFT+VENV HAH+CA EALD
Sbjct: 183 CALRPSNVFGPGDTQLVPLLVNLAKPGWTKFIIGSGENMSDFTYVENVAHAHVCAAEALD 242

Query: 247 FRMVSVAGKAFFITNFKPMKFWEFISLVLEGLGYRRPSVKLPFKMVWNVLLFMKWIDEKF 306
            RMVSVAG AFFITN +P+KFW+F+S++LEGLGY+RP +KLP  +VW ++L +KWI EK 
Sbjct: 243 SRMVSVAGMAFFITNLEPIKFWDFLSIILEGLGYQRPFIKLPTGVVWYIILLVKWIHEKL 302

Query: 307 GFNKYNHSTWAHYI-QLASCTRTFNCVAAHKQIGYSPIVSLEEGITLTIKSFSHLRKELS 366
           G   YNHS  A YI QLAS TRTF+C+AA K IGYSP+VSLEEG++ TI+SFSHL ++ S
Sbjct: 303 GLRTYNHSLSACYIVQLASRTRTFDCIAAQKHIGYSPVVSLEEGVSSTIQSFSHLARDSS 362

Query: 367 LASFTEFTDESKADKLLGGGR 387
           LA   +F ++SK +KLLGGG+
Sbjct: 363 LAYSRDFNEQSKVEKLLGGGK 383

BLAST of Lsi05G013430 vs. TAIR10
Match: AT2G43420.1 (AT2G43420.1 3-beta hydroxysteroid dehydrogenase/isomerase family protein)

HSP 1 Score: 431.4 bits (1108), Expect = 8.1e-121
Identity = 225/386 (58.29%), Postives = 279/386 (72.28%), Query Frame = 1

Query: 1   MEVDDRFVELSTKTCVVLGGRGFLGRSLVLRLLKLGKWNVRIADSAESLELDPSEHDSLL 60
           M+ D    +   KTCVVLGGRGF+GRSLV RLL+LG W VR+ADS  +L LD  E DSLL
Sbjct: 1   MDEDSVHGDSHLKTCVVLGGRGFIGRSLVSRLLRLGNWTVRVADSGHTLHLD--ESDSLL 60

Query: 61  SDALIAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVI 120
            DAL +GRASY  VDVR+K QIV    GS VVFYM   D  S+D +  Y  IVQG +NVI
Sbjct: 61  EDALSSGRASYHCVDVRDKPQIVKVTEGSYVVFYMGATDLRSHDYFDCYKVIVQGTRNVI 120

Query: 121 NACRDCKVRRLIFNSSADVIFDGSRDIRNGEESLTYPWKFEDMLSDLKAQAEALILFAND 180
           +ACR+  VR+LI+NS+ADV+FDGS+ IR+G+ESL  P KF+ ML+D KAQAEALI  AN+
Sbjct: 121 SACRESGVRKLIYNSTADVVFDGSQPIRDGDESLRRPLKFQSMLTDFKAQAEALIKLANN 180

Query: 181 IDGLLTCALRPSNVFGPEDTRLVPFMVHQAKSGLAKFIIGNGENMSDFTFVENVTHAHIC 240
            DGLLTCALR S VFGP DT  VPF+V+ AKSG AKFI+G+GEN+SDFT+ ENV+HAHIC
Sbjct: 181 RDGLLTCALRSSIVFGPGDTEFVPFLVNLAKSGYAKFILGSGENISDFTYSENVSHAHIC 240

Query: 241 AEEALDFRMVSVAGKAFFITNFKPMKFWEFISLVLEGLGYRRPSVKLPFKMVWNVLLFMK 300
           A +ALD +M  VAGK FFITN KP++FW+F+S ++EGLGY RPS+KLP ++V  V   +K
Sbjct: 241 AVKALDSQMEFVAGKEFFITNLKPVRFWDFVSHIVEGLGYPRPSIKLPVRLVLYVFSLLK 300

Query: 301 WIDEKFGFNKYNHSTWAHYIQLASCTRTFNCVAAHKQIGYSPIVSLEEGITLTIKSFSHL 360
           W  EK G    N+ T   Y  LAS TRTFNC AA K +GY+P+V+LE+GI  T++ FS  
Sbjct: 301 WTHEKEGLGS-NYDTAHQYALLASSTRTFNCNAAKKHLGYTPVVTLEDGIASTLQWFSR- 360

Query: 361 RKELSLASFTEFTDESKADKLLGGGR 387
                L    +   +S AD+LLG G+
Sbjct: 361 ----DLEKSDDTIIQSTADQLLGCGK 378

BLAST of Lsi05G013430 vs. TAIR10
Match: AT2G26260.1 (AT2G26260.1 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2)

HSP 1 Score: 327.8 bits (839), Expect = 1.3e-89
Identity = 174/376 (46.28%), Postives = 240/376 (63.83%), Query Frame = 1

Query: 15  CVVLGGRGFLGRSLVLRLLKLGKWNVRIADSAESLELDPSEHDSLLSDALIAGRASYFHV 74
           CVV GGRGF  R LV  L++   + VRIAD A ++ LDP E + +L + L +GR  Y   
Sbjct: 12  CVVTGGRGFAARHLVEMLVRYEMFCVRIADLAPAIMLDPQEGNGVLDEGLRSGRVQYISA 71

Query: 75  DVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINACRDCKVRRLIFN 134
           D+R+K Q+V A  G+ VVF+M   DS  N+  L Y   VQG +NVI+AC D  V+RLI+ 
Sbjct: 72  DLRDKSQVVKAFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTQNVIDACVDVGVKRLIYT 131

Query: 135 SSADVIFDGSRDIRNGEESLTYPWKFEDMLSDLKAQAEALILFANDIDGLLTCALRPSNV 194
           SS  V+FDG   I NG ES+ YP K  D  S  KA+ E LI+ AN  +GLLTC +RPS++
Sbjct: 132 SSPSVVFDGVHGILNGTESMAYPIKHNDSYSATKAEGEELIMKANGRNGLLTCCIRPSSI 191

Query: 195 FGPEDTRLVPFMVHQAKSGLAKFIIGNGENMSDFTFVENVTHAHICAEEALDFR---MVS 254
           FGP D  LVP +V  A++G +KFIIG+G N+ DFT+VENV HAH+CAE AL         
Sbjct: 192 FGPGDRLLVPSLVAAARAGKSKFIIGDGNNLYDFTYVENVAHAHVCAERALASGGDVSTK 251

Query: 255 VAGKAFFITNFKPMKFWEFISLVLEGLGYRRPSVKLPFKMVWNVLLFMKWIDEKFG-FNK 314
            AG+A+FITN +P+KFWEF+S +L+GLGY RPS+K+P  ++  +   ++   +  G +  
Sbjct: 252 AAGQAYFITNMEPIKFWEFMSQLLDGLGYERPSIKIPAFIMMPIAHLVELTYKVLGPYGM 311

Query: 315 YNHSTWAHYIQLASCTRTFNCVAAHKQIGYSPIVSLEEGITLTIKSFSHLRKELSLASFT 374
                    ++L SC+RTF+   A  ++GY+P+V L+EGI  TI SFSH    L+  S +
Sbjct: 312 TVPQLTPSRVRLLSCSRTFDSTKAKDRLGYAPVVPLQEGIRRTIDSFSH----LTAGSQS 371

Query: 375 EFTDESKADKLLGGGR 387
           +    SKA ++LGGG+
Sbjct: 372 KREGPSKASRILGGGK 383

BLAST of Lsi05G013430 vs. TAIR10
Match: AT1G47290.2 (AT1G47290.2 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1)

HSP 1 Score: 318.9 bits (816), Expect = 5.8e-87
Identity = 166/353 (47.03%), Postives = 224/353 (63.46%), Query Frame = 1

Query: 15  CVVLGGRGFLGRSLVLRLLKLGKWNVRIADSAESLELDPSEHDSLLSDALIAGRASYFHV 74
           CVV GGRGF  R LV  L++   ++VRIAD A ++ L+P E   +L +A+ +GR  Y   
Sbjct: 12  CVVTGGRGFAARHLVEMLVRYQMFHVRIADLAPAIVLNPHEETGILGEAIRSGRVQYVSA 71

Query: 75  DVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINACRDCKVRRLIFN 134
           D+RNK Q+V    G+ VVF+M   DS  N+  L Y   VQG  NVI+AC +  V+RLI+ 
Sbjct: 72  DLRNKTQVVKGFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTTNVIDACIEVGVKRLIYT 131

Query: 135 SSADVIFDGSRDIRNGEESLTYPWKFEDMLSDLKAQAEALILFANDIDGLLTCALRPSNV 194
           SS  V+FDG     N +ESL YP K  D  S  KA+ EALIL AN   GLLTC +RPS++
Sbjct: 132 SSPSVVFDGVHGTLNADESLPYPPKHNDSYSATKAEGEALILKANGRSGLLTCCIRPSSI 191

Query: 195 FGPEDTRLVPFMVHQAKSGLAKFIIGNGENMSDFTFVENVTHAHICAEEALDFR---MVS 254
           FGP D  +VP +V  A++G +KFIIG+G N  DFT+VENV HAH+CAE AL         
Sbjct: 192 FGPGDKLMVPSLVTAARAGKSKFIIGDGSNFYDFTYVENVVHAHVCAERALASGGEVCAK 251

Query: 255 VAGKAFFITNFKPMKFWEFISLVLEGLGYRRPSVKLPFKMVWNVLLFMKWIDEKFG-FNK 314
            AG+A+FITN +P+KFWEF+S +LEGLGY RPS+K+P  ++  +   ++   +  G +  
Sbjct: 252 AAGQAYFITNMEPIKFWEFMSQLLEGLGYERPSIKIPASLMMPIAYLVELAYKLLGPYGM 311

Query: 315 YNHSTWAHYIQLASCTRTFNCVAAHKQIGYSPIVSLEEGITLTIKSFSHLRKE 364
                    ++L SC RTF+   A  ++GYSP+V L+EGI  TI SFSHL+ +
Sbjct: 312 KVPVLTPSRVRLLSCNRTFDSSKAKDRLGYSPVVPLQEGIKRTIDSFSHLKAQ 364

BLAST of Lsi05G013430 vs. TAIR10
Match: AT2G33630.1 (AT2G33630.1 NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 122.1 bits (305), Expect = 1.0e-27
Identity = 115/390 (29.49%), Postives = 176/390 (45.13%), Query Frame = 1

Query: 14  TCVVLGGRGFLGRSLVLRLLKLGKWNVRIADSAESLELDPSEHDSLLSDALIAGRASYFH 73
           T VV GG GF+G +L L L++ G   VR  D           H S  SD L         
Sbjct: 13  TFVVTGGLGFVGAALCLELVRRGARQVRSFDL---------RHSSPWSDDLKNSGVRCIQ 72

Query: 74  VDVRNKDQIVAALSGSSVVFYM-DYEDSHSNDIYLSYM--FIVQGAKNVINACRDCKVRR 133
            DV  K  +  AL G+  V ++  Y  S    +         + G  NV+ A    ++ R
Sbjct: 73  GDVTKKQDVDNALDGADCVLHLASYGMSGKEMLRFGRCDEVNINGTCNVLEAAFKHEITR 132

Query: 134 LIFNSSADVIFDGSRDIRNGEESLTY-PWKFE-DMLSDLKAQAEALIL------FANDID 193
           +++ S+ +V+F G ++I NG E L Y P     D  S  K+ AE L+L      F N   
Sbjct: 133 IVYVSTYNVVF-GGKEILNGNEGLPYFPLDDHVDAYSRTKSIAEQLVLKSNGRPFKNGGK 192

Query: 194 GLLTCALRPSNVFGPEDTRLVPFMVHQAKSGLAKFIIGNGENMSDFTFVENVTHAHICAE 253
            + TCA+RP+ ++GP + R +P +V   K GLA F IG     SD+ +VEN+  A I A 
Sbjct: 193 RMYTCAIRPAAIYGPGEDRHLPRIVTLTKLGLALFKIGEPSVKSDWIYVENLVLAIILAS 252

Query: 254 EALDFRMVS------VAGKAFFITNFKPMKFWEFISLVLEGLGYRRP--SVKLPFKM--- 313
             L   +         AG+ +F+++  P+  +EF+  +L+ L Y  P  ++ +PF +   
Sbjct: 253 MGLLDDIPGREGQPVAAGQPYFVSDGYPVNTFEFLRPLLKSLDYDLPKCTISVPFALSLG 312

Query: 314 -----VWNVL---LFMKWIDEKFGFNKYNHSTWAHYIQLASCTRTFNCVAAHKQIGYSPI 373
                 + VL   L   W+ +               +     T  F+ + A +++GY P 
Sbjct: 313 KIFQGFYTVLYPWLSKSWLPQPL--------VLPAEVYKVGVTHYFSYLKAKEELGYVPF 372

BLAST of Lsi05G013430 vs. NCBI nr
Match: gi|659077177|ref|XP_008439072.1| (PREDICTED: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 3 isoform X2 [Cucumis melo])

HSP 1 Score: 761.1 bits (1964), Expect = 1.3e-216
Identity = 373/386 (96.63%), Postives = 381/386 (98.70%), Query Frame = 1

Query: 1   MEVDDRFVELSTKTCVVLGGRGFLGRSLVLRLLKLGKWNVRIADSAESLELDPSEHDSLL 60
           MEVDDRF+EL+TKTCVVLGGRGFLGRSLVLRLLKLGKWNVRIADSA SLELDPSEHDSLL
Sbjct: 1   MEVDDRFLELNTKTCVVLGGRGFLGRSLVLRLLKLGKWNVRIADSANSLELDPSEHDSLL 60

Query: 61  SDALIAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVI 120
           SDALIAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVI
Sbjct: 61  SDALIAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVI 120

Query: 121 NACRDCKVRRLIFNSSADVIFDGSRDIRNGEESLTYPWKFEDMLSDLKAQAEALILFAND 180
           NACRDCKVRRLIFNSSADVIFDGSRDI NGEESLTYPWKFEDMLSDLKAQAEALILFAND
Sbjct: 121 NACRDCKVRRLIFNSSADVIFDGSRDILNGEESLTYPWKFEDMLSDLKAQAEALILFAND 180

Query: 181 IDGLLTCALRPSNVFGPEDTRLVPFMVHQAKSGLAKFIIGNGENMSDFTFVENVTHAHIC 240
           IDGLLTCALRPSNVFGPEDTRLVPF+VHQAKSGLAK IIGNGENMSDFTFVENVTHAHIC
Sbjct: 181 IDGLLTCALRPSNVFGPEDTRLVPFVVHQAKSGLAKLIIGNGENMSDFTFVENVTHAHIC 240

Query: 241 AEEALDFRMVSVAGKAFFITNFKPMKFWEFISLVLEGLGYRRPSVKLPFKMVWNVLLFMK 300
           AEEALDFRMVSVAGKAFFITNFKPMKFW+FISL+LEGLGYRRPSVKLP K+VWNVLLFMK
Sbjct: 241 AEEALDFRMVSVAGKAFFITNFKPMKFWQFISLILEGLGYRRPSVKLPCKVVWNVLLFMK 300

Query: 301 WIDEKFGFNKYNHSTWAHYIQLASCTRTFNCVAAHKQIGYSPIVSLEEGITLTIKSFSHL 360
           WIDEKFGFNKYNHSTWAHYIQLASCTRTFNC AAHKQ+GYSP+VSLEEGITLTIKSFSHL
Sbjct: 301 WIDEKFGFNKYNHSTWAHYIQLASCTRTFNCSAAHKQLGYSPVVSLEEGITLTIKSFSHL 360

Query: 361 RKELSLASFTEFTDESKADKLLGGGR 387
           RKELSLASFTEFTDESKADKLLGGGR
Sbjct: 361 RKELSLASFTEFTDESKADKLLGGGR 386

BLAST of Lsi05G013430 vs. NCBI nr
Match: gi|659077174|ref|XP_008439071.1| (PREDICTED: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 3 isoform X1 [Cucumis melo])

HSP 1 Score: 761.1 bits (1964), Expect = 1.3e-216
Identity = 373/386 (96.63%), Postives = 381/386 (98.70%), Query Frame = 1

Query: 1   MEVDDRFVELSTKTCVVLGGRGFLGRSLVLRLLKLGKWNVRIADSAESLELDPSEHDSLL 60
           MEVDDRF+EL+TKTCVVLGGRGFLGRSLVLRLLKLGKWNVRIADSA SLELDPSEHDSLL
Sbjct: 1   MEVDDRFLELNTKTCVVLGGRGFLGRSLVLRLLKLGKWNVRIADSANSLELDPSEHDSLL 60

Query: 61  SDALIAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVI 120
           SDALIAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVI
Sbjct: 61  SDALIAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVI 120

Query: 121 NACRDCKVRRLIFNSSADVIFDGSRDIRNGEESLTYPWKFEDMLSDLKAQAEALILFAND 180
           NACRDCKVRRLIFNSSADVIFDGSRDI NGEESLTYPWKFEDMLSDLKAQAEALILFAND
Sbjct: 121 NACRDCKVRRLIFNSSADVIFDGSRDILNGEESLTYPWKFEDMLSDLKAQAEALILFAND 180

Query: 181 IDGLLTCALRPSNVFGPEDTRLVPFMVHQAKSGLAKFIIGNGENMSDFTFVENVTHAHIC 240
           IDGLLTCALRPSNVFGPEDTRLVPF+VHQAKSGLAK IIGNGENMSDFTFVENVTHAHIC
Sbjct: 181 IDGLLTCALRPSNVFGPEDTRLVPFVVHQAKSGLAKLIIGNGENMSDFTFVENVTHAHIC 240

Query: 241 AEEALDFRMVSVAGKAFFITNFKPMKFWEFISLVLEGLGYRRPSVKLPFKMVWNVLLFMK 300
           AEEALDFRMVSVAGKAFFITNFKPMKFW+FISL+LEGLGYRRPSVKLP K+VWNVLLFMK
Sbjct: 241 AEEALDFRMVSVAGKAFFITNFKPMKFWQFISLILEGLGYRRPSVKLPCKVVWNVLLFMK 300

Query: 301 WIDEKFGFNKYNHSTWAHYIQLASCTRTFNCVAAHKQIGYSPIVSLEEGITLTIKSFSHL 360
           WIDEKFGFNKYNHSTWAHYIQLASCTRTFNC AAHKQ+GYSP+VSLEEGITLTIKSFSHL
Sbjct: 301 WIDEKFGFNKYNHSTWAHYIQLASCTRTFNCSAAHKQLGYSPVVSLEEGITLTIKSFSHL 360

Query: 361 RKELSLASFTEFTDESKADKLLGGGR 387
           RKELSLASFTEFTDESKADKLLGGGR
Sbjct: 361 RKELSLASFTEFTDESKADKLLGGGR 386

BLAST of Lsi05G013430 vs. NCBI nr
Match: gi|449459852|ref|XP_004147660.1| (PREDICTED: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 3 [Cucumis sativus])

HSP 1 Score: 754.2 bits (1946), Expect = 1.5e-214
Identity = 369/386 (95.60%), Postives = 379/386 (98.19%), Query Frame = 1

Query: 1   MEVDDRFVELSTKTCVVLGGRGFLGRSLVLRLLKLGKWNVRIADSAESLELDPSEHDSLL 60
           MEVDDRF+EL+TKTCVVLGGRGFLGRSLVLRLLKL KWNVRIADSA SLELDPSEHDSLL
Sbjct: 1   MEVDDRFLELNTKTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSANSLELDPSEHDSLL 60

Query: 61  SDALIAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVI 120
           SDALIAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVI
Sbjct: 61  SDALIAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVI 120

Query: 121 NACRDCKVRRLIFNSSADVIFDGSRDIRNGEESLTYPWKFEDMLSDLKAQAEALILFAND 180
           +ACRDCKVRRLIFNSSADVIFDGSRDI NGEESLTYPWKFEDMLSDLKAQAEALILFAND
Sbjct: 121 SACRDCKVRRLIFNSSADVIFDGSRDILNGEESLTYPWKFEDMLSDLKAQAEALILFAND 180

Query: 181 IDGLLTCALRPSNVFGPEDTRLVPFMVHQAKSGLAKFIIGNGENMSDFTFVENVTHAHIC 240
           IDGLLTCALRPSNVFGPEDTRLVPF+VHQAKSG AKFIIGNGENMSDFTFVENVTHAHIC
Sbjct: 181 IDGLLTCALRPSNVFGPEDTRLVPFIVHQAKSGFAKFIIGNGENMSDFTFVENVTHAHIC 240

Query: 241 AEEALDFRMVSVAGKAFFITNFKPMKFWEFISLVLEGLGYRRPSVKLPFKMVWNVLLFMK 300
           AEEALDFRMVSVAGKAFFITNFKPMKFWEFISL+L GLGYRRPSVKLP K+VWNVLLFMK
Sbjct: 241 AEEALDFRMVSVAGKAFFITNFKPMKFWEFISLILRGLGYRRPSVKLPSKVVWNVLLFMK 300

Query: 301 WIDEKFGFNKYNHSTWAHYIQLASCTRTFNCVAAHKQIGYSPIVSLEEGITLTIKSFSHL 360
           WIDEKFGFNKYNHSTWAHYIQLASCTRTF+C AAHKQ+GYSP+VSLEEGITLTIKSFSHL
Sbjct: 301 WIDEKFGFNKYNHSTWAHYIQLASCTRTFSCAAAHKQLGYSPVVSLEEGITLTIKSFSHL 360

Query: 361 RKELSLASFTEFTDESKADKLLGGGR 387
           RKELSLASFTEFT+ESKADKLLGGGR
Sbjct: 361 RKELSLASFTEFTEESKADKLLGGGR 386

BLAST of Lsi05G013430 vs. NCBI nr
Match: gi|147801865|emb|CAN74982.1| (hypothetical protein VITISV_007871 [Vitis vinifera])

HSP 1 Score: 565.1 bits (1455), Expect = 1.3e-157
Identity = 301/532 (56.58%), Postives = 382/532 (71.80%), Query Frame = 1

Query: 8   VELSTKTCVVLGGRGFLGRSLVLRLLKLGKWNVRIADSAESLELDPSEHDSLLSDALIAG 67
           ++ + KTCVV GGRGF+GR LVLRLLKLGKW VR+ADSA+SL+LDPSE  S+LS+A+ +G
Sbjct: 3   IDENLKTCVVFGGRGFIGRFLVLRLLKLGKWIVRVADSAQSLQLDPSEDRSVLSEAISSG 62

Query: 68  RASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINACRDCK 127
           RAS   VDVR+K Q+  A+ G+SVVFYMD   +++ND YL YM IVQG +NVINAC++CK
Sbjct: 63  RASCCAVDVRDKAQVRKAIEGASVVFYMDPTITYTNDFYLCYMIIVQGVRNVINACQECK 122

Query: 128 VRRLIFNSSADVIFDGSRDIRNGEESLTYPWKFEDMLSDLKAQAEALILFANDIDGLLTC 187
           V+RLI+NSSADV+FDGS DI NG+ESL  PWKFEDML+D+KAQAE L+L AN+IDGL+TC
Sbjct: 123 VKRLIYNSSADVVFDGSHDIYNGDESLPCPWKFEDMLTDIKAQAEGLVLIANNIDGLVTC 182

Query: 188 ALRPSNVFGPEDTRLVPFMVHQAKSGLAKFIIGNGENMSDFTFVENVTHAHICAEEALDF 247
           ALRP NVFGP D +LVPF+V++AKSG AKF+IG+GENMSDFT+VENV HAH+CAEEAL  
Sbjct: 183 ALRPCNVFGPGDKQLVPFLVNKAKSGHAKFVIGSGENMSDFTYVENVAHAHVCAEEALVS 242

Query: 248 RMVSVAGKAFFITNFKPMKFWEFISLVLEGLGYRRPSVKLPFKMVWNVLLFMKWIDEKFG 307
           RMVSVAGK FFITN +P+KFWEF+SL+LEGLGY+RP  KLP  MV  VL  ++W+ +K  
Sbjct: 243 RMVSVAGKVFFITNLEPVKFWEFVSLILEGLGYQRPLFKLPAGMVLYVLSLVEWMRDKLD 302

Query: 308 FNKYNHSTWAHY-IQLASCTRTFNCVAAHKQIGYSPIVSLEEGITLTIKSFSHLRKELSL 367
           F + NH   A Y +Q+AS TRTFNC AA K IGYSP+VSLEEG+TLT +SFS L ++ S+
Sbjct: 303 F-RSNHPVSAQYVVQIASRTRTFNCSAAQKHIGYSPVVSLEEGVTLTTESFSQLSQDSSV 362

Query: 368 ASFTEFTDESKADKLLGGGR--------AFRQSDSVVRDSI-------MTLACL-WNRGV 427
            +  +F ++SK DKLLGGG+        A      ++  +I       ++L+C   +  V
Sbjct: 363 MALRDFDEQSKVDKLLGGGKAKLLLLVTAILSGQGILPTNIFGFSIQRISLSCFEISXTV 422

Query: 428 HNARSLARGEDWNYFFKEVLQQCSDDNIDLTAFVPAVAFLYILKLLLSQSLTMLLGMGKQ 487
            N         WN +   +         D   F      LY LKL+LSQ LT+++G    
Sbjct: 423 VNDLITNMANLWNNWIHMIRLLAGGG--DWNTFFKVAIPLYFLKLILSQCLTLVVG---- 482

Query: 488 QLGLVFAFTAFFVYEQYEAEIDEFAKFFFIGIMELKRLLASHLPTPLMSLLC 523
            + LV AFT+FFVYEQYE E+D  A+  F  I + KRLL  +LP PL+S LC
Sbjct: 483 -VALVLAFTSFFVYEQYEEEMDGLAEVLFDSIKKSKRLLMRNLPAPLVSFLC 526

BLAST of Lsi05G013430 vs. NCBI nr
Match: gi|1009139206|ref|XP_015887000.1| (PREDICTED: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 3-like isoform X3 [Ziziphus jujuba])

HSP 1 Score: 555.4 bits (1430), Expect = 1.1e-154
Identity = 278/388 (71.65%), Postives = 323/388 (83.25%), Query Frame = 1

Query: 1   MEVDDRFVELS--TKTCVVLGGRGFLGRSLVLRLLKLGKWNVRIADSAESLELDPSEHDS 60
           MEVDDR  EL+  T+TCVVL GRGFLGRSLVL+LL+LGKW VRIADSA+SL+LDPSE DS
Sbjct: 1   MEVDDRLAELNPKTRTCVVLEGRGFLGRSLVLKLLELGKWIVRIADSAQSLQLDPSESDS 60

Query: 61  LLSDALIAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKN 120
            LS A+  GRASY  VDVRN+  I+ A+ GSSVVFYMD  D  ++D YL YM IVQGAKN
Sbjct: 61  SLSHAISKGRASYHCVDVRNRSLILKAVEGSSVVFYMDDADLRAHDFYLCYMIIVQGAKN 120

Query: 121 VINACRDCKVRRLIFNSSADVIFDGSRDIRNGEESLTYPWKFEDMLSDLKAQAEALILFA 180
           VINACRD KVRRLI++SSADVIF+GS DI NG+ESL YP KFE++LSDLKAQAEALIL A
Sbjct: 121 VINACRDGKVRRLIYSSSADVIFNGSCDINNGDESLRYPGKFENILSDLKAQAEALILHA 180

Query: 181 NDIDGLLTCALRPSNVFGPEDTRLVPFMVHQAKSGLAKFIIGNGENMSDFTFVENVTHAH 240
           NDIDGLLTCA+RP NVFGP DT LVP++V+ AKSG AKFIIGNGENMSDFT+VENV+HAH
Sbjct: 181 NDIDGLLTCAIRPCNVFGPGDTHLVPYLVNLAKSGWAKFIIGNGENMSDFTYVENVSHAH 240

Query: 241 ICAEEALDFRMVSVAGKAFFITNFKPMKFWEFISLVLEGLGYRRPSVKLPFKMVWNVLLF 300
           ICAEEALDFR  SVAGKAFFITN  PMKFW+F+S +LEGLGY+RP +KLP +MV +V +F
Sbjct: 241 ICAEEALDFRTSSVAGKAFFITNLDPMKFWDFVSHILEGLGYQRPFIKLPARMVLDVFMF 300

Query: 301 MKWIDEKFGFNKYNHSTWAHYIQLASCTRTFNCVAAHKQIGYSPIVSLEEGITLTIKSFS 360
           +K + EKFGF +YNHS   HY+ L SC+RTFNC AA K IGYSPIVSL+EGI LTI SFS
Sbjct: 301 IKRMHEKFGFIRYNHSLLVHYVHLGSCSRTFNCNAAQKHIGYSPIVSLQEGIALTIGSFS 360

Query: 361 HLRKELSLASFTEFTDESKADKLLGGGR 387
           HL K++S +  ++  + SKADKLLG G+
Sbjct: 361 HLAKDMSFSRCSDLDEPSKADKLLGSGK 388

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
HSDD3_ARATH1.4e-11958.293beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 3 OS=Arabidopsis thalia... [more]
HSDD2_ARATH2.2e-8846.283beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 OS=Arabidopsis thalia... [more]
HSDD1_ARATH1.0e-8547.033beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 OS=Arabidopsis thalia... [more]
NSDHL_RAT6.3e-5937.39Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating OS=Rattus norvegicus... [more]
NSDHL_MOUSE4.1e-5837.68Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating OS=Mus musculus GN=N... [more]
Match NameE-valueIdentityDescription
A0A0A0L5K0_CUCSA1.1e-21495.60Reticulon-like protein OS=Cucumis sativus GN=Csa_3G174040 PE=3 SV=1[more]
A5BT18_VITVI9.2e-15856.58Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_007871 PE=3 SV=1[more]
M5XMU8_PRUPE8.9e-15370.03Reticulon-like protein OS=Prunus persica GN=PRUPE_ppa003251mg PE=3 SV=1[more]
B9SU06_RICCO6.9e-14565.89Reticulon-like protein OS=Ricinus communis GN=RCOM_0454370 PE=3 SV=1[more]
A0A067DQ35_CITSI4.5e-14465.88Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g007866mg PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G43420.18.1e-12158.29 3-beta hydroxysteroid dehydrogenase/isomerase family protein[more]
AT2G26260.11.3e-8946.28 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2[more]
AT1G47290.25.8e-8747.03 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1[more]
AT2G33630.11.0e-2729.49 NAD(P)-binding Rossmann-fold superfamily protein[more]
Match NameE-valueIdentityDescription
gi|659077177|ref|XP_008439072.1|1.3e-21696.63PREDICTED: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 3 isoform X2... [more]
gi|659077174|ref|XP_008439071.1|1.3e-21696.63PREDICTED: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 3 isoform X1... [more]
gi|449459852|ref|XP_004147660.1|1.5e-21495.60PREDICTED: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 3 [Cucumis s... [more]
gi|147801865|emb|CAN74982.1|1.3e-15756.58hypothetical protein VITISV_007871 [Vitis vinifera][more]
gi|1009139206|ref|XP_015887000.1|1.1e-15471.65PREDICTED: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 3-like isofo... [more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO:0006694steroid biosynthetic process
Vocabulary: Molecular Function
TermDefinition
GO:00038543-beta-hydroxy-delta5-steroid dehydrogenase activity
GO:0016616oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
Vocabulary: INTERPRO
TermDefinition
IPR016040NAD(P)-bd_dom
IPR0022253Beta_OHSteriod_DH/Estase
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008209 androgen metabolic process
biological_process GO:0008207 C21-steroid hormone metabolic process
biological_process GO:0008210 estrogen metabolic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0006694 steroid biosynthetic process
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0009506 plasmodesma
cellular_component GO:0005575 cellular_component
molecular_function GO:0003854 3-beta-hydroxy-delta5-steroid dehydrogenase activity
molecular_function GO:0047012 sterol-4-alpha-carboxylate 3-dehydrogenase (decarboxylating) activity
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G013430.1Lsi05G013430.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0022253-beta hydroxysteroid dehydrogenase/isomerasePFAMPF010733Beta_HSDcoord: 16..288
score: 2.0
IPR016040NAD(P)-binding domainGENE3DG3DSA:3.40.50.720coord: 14..354
score: 1.6
IPR016040NAD(P)-binding domainunknownSSF51735NAD(P)-binding Rossmann-fold domainscoord: 10..296
score: 1.45E-49coord: 326..360
score: 1.45
NoneNo IPR availablePANTHERPTHR10366NAD DEPENDENT EPIMERASE/DEHYDRATASEcoord: 14..309
score: 2.9E-195coord: 326..380
score: 2.9E
NoneNo IPR availablePANTHERPTHR10366:SF3863BETA-HYDROXYSTEROID-DEHYDROGENASE/DECARBOXYLASE ISOFORM 3coord: 14..309
score: 2.9E-195coord: 326..380
score: 2.9E