Lsi05G013390 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi05G013390
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Strictosidine synthase family protein
Location	chr05 : 21242714 .. 21244152 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGAAAAAAGGAGTAGTGAAGGAAGAATCTTTGCTTCAGCACCCAGTACTTTTCTTGCTAGTTTTGGGCCTGGGTTTTGTAGTAATGGACCCATTTGAGATGGGTCCTGTTGGAGGCCATGATTTCAGGCCTGTGAAGCATGACATTGCACCTTACAGCCAGGTTATGGGCCGTTGGCCCAAGGACAATGAGAGTCGTTTGGGCCATGGAAATTTGGAATTTGAAGATGAGGTTTTCGGGCCTGAGTCCTTGGAGTTTGATGCATTGGGCCGTGGGCCTTACACTGGGCTTGCCGATGGCCGCATTGTTAGATGGATGGGAAAGGAAGTTGGATGGGAGACATTTGCAATTGTGACAACAAATTGGTATATTTTATTTAGAATATAGTTTTCCTTTTAAATTCTAAAATTAAATTGATTTTTTTTTTGTAAATTTTGGGATTGAAATTCAGGTCAGAGAAGGTATGTGGTAAAGGGGTTGATTCAACAACAGCAAAACAATGGAAAAATGAGAAGAAATGTGGAAGGCCACTTGGGCTAAGATTTGAGAAAGAAAGTGGGAATTTGTACATAGCTGATGCATATTATGGGCTTCTTGTTGTTGGGCCTCAAGGGGGAATTGCCACTCCCTTAGCCACTCATGTTGAAGGAACTCCCATTCTCTTTGCTAATGATCTCGATATCCACAACAATGGCTCCATCTTCTTCACAGACACTAGCAAGAGATATAATAGAGTGTAACTACTGATTTACATGACCTTTTAACTAAATTAATCGTTTAAATACTACTTTGGTTTCCGTATTTTTGTTTTTGGTTCATTTTAGTCTTTATACTTTCAAAATATTTATTTTGGACCCTATATTTTCAACTTTGTTTCATTTTGGTCCTTGTATTTTTAAAAAATAACTATTTTGGGTCCCTCTATTTTCATTTTTACTTCATTTTTAAGGTATCAAAATTGTCACTTTTTTAAAGTTGACCAAAATAAACAAAAGTTGAAAGTTCAAATACCAAAATGAACATTTTGAAAGTACATGAACCAAAATGAACCAAAGTTAAAATATAGGGACCATAATGAACATTTTAAAAGTATAGGTAACAAAATGAACCAAAGCCAAAAGTACAGGAACCGAAGTAGTATTTAAACCTAAATTAATTTATATCTAATAATAAGAAAAACTATTTACAGAAAATAGCAAAATTGTTAGATAGTTGTGATAGATGCTGATAGAAGTCTGTCAATGTCTATCAATGTTTTTCTTTTACTATTTTCCGTAAATAGTTTGACATTTTTTCTATCTGTAAAAATTTTCCTTTTTAATAATGTCACTTTCGTCGTGTTTTTGATTATGTGTTTGTTGTTTGATATAGGGATCATTTCTTCATATTGTTGGAAGGAGAAGCTTCAGGTAGGCTTCTTAGATATGATCCTTTAA

mRNA sequence

ATGGAGAAAAAAGGAGTAGTGAAGGAAGAATCTTTGCTTCAGCACCCAGTACTTTTCTTGCTAGTTTTGGGCCTGGGTTTTGTAGTAATGGACCCATTTGAGATGGGTCCTGTTGGAGGCCATGATTTCAGGCCTGTGAAGCATGACATTGCACCTTACAGCCAGGTTATGGGCCGTTGGCCCAAGGACAATGAGAGTCGTTTGGGCCATGGAAATTTGGAATTTGAAGATGAGGTTTTCGGGCCTGAGTCCTTGGAGTTTGATGCATTGGGCCGTGGGCCTTACACTGGGCTTGCCGATGGCCGCATTGTTAGATGGATGGGAAAGGAAGTTGGATGGGAGACATTTGCAATTGTATGTGGTAAAGGGGTTGATTCAACAACAGCAAAACAATGGAAAAATGAGAAGAAATGTGGAAGGCCACTTGGGCTAAGATTTGAGAAAGAAAGTGGGAATTTGTACATAGCTGATGCATATTATGGGCTTCTTGTTGTTGGGCCTCAAGGGGGAATTGCCACTCCCTTAGCCACTCATGAGAAGCTTCAGGTAGGCTTCTTAGATATGATCCTTTAA

Coding sequence (CDS)

ATGGAGAAAAAAGGAGTAGTGAAGGAAGAATCTTTGCTTCAGCACCCAGTACTTTTCTTGCTAGTTTTGGGCCTGGGTTTTGTAGTAATGGACCCATTTGAGATGGGTCCTGTTGGAGGCCATGATTTCAGGCCTGTGAAGCATGACATTGCACCTTACAGCCAGGTTATGGGCCGTTGGCCCAAGGACAATGAGAGTCGTTTGGGCCATGGAAATTTGGAATTTGAAGATGAGGTTTTCGGGCCTGAGTCCTTGGAGTTTGATGCATTGGGCCGTGGGCCTTACACTGGGCTTGCCGATGGCCGCATTGTTAGATGGATGGGAAAGGAAGTTGGATGGGAGACATTTGCAATTGTATGTGGTAAAGGGGTTGATTCAACAACAGCAAAACAATGGAAAAATGAGAAGAAATGTGGAAGGCCACTTGGGCTAAGATTTGAGAAAGAAAGTGGGAATTTGTACATAGCTGATGCATATTATGGGCTTCTTGTTGTTGGGCCTCAAGGGGGAATTGCCACTCCCTTAGCCACTCATGAGAAGCTTCAGGTAGGCTTCTTAGATATGATCCTTTAA

Protein sequence

MEKKGVVKEESLLQHPVLFLLVLGLGFVVMDPFEMGPVGGHDFRPVKHDIAPYSQVMGRWPKDNESRLGHGNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMGKEVGWETFAIVCGKGVDSTTAKQWKNEKKCGRPLGLRFEKESGNLYIADAYYGLLVVGPQGGIATPLATHEKLQVGFLDMIL

Homology

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BLAST of Lsi05G013390 vs. Swiss-Prot
Match: SSL13_ARATH (Protein STRICTOSIDINE SYNTHASE-LIKE 13 OS=Arabidopsis thaliana GN=SSL13 PE=1 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 2.5e-74
Identity = 128/189 (67.72%), Postives = 153/189 (80.95%), Query Frame = 1


Query: 1   MEKKGV--VKEESLLQHPVLFLLVLGLGFVVMDPFEMGPVGGHDFRPVKHDIAPYSQVMG 60
           MEKKG     E  +  HP+L ++ L + F+ +DPF M P+GG +F+PVKH++APY +VMG
Sbjct: 1   MEKKGQHGTYESMMTHHPILCIIALSVLFIAIDPFHMSPIGGREFKPVKHEVAPYKEVMG 60

Query: 61  RWPKDNESRLG-HGNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMGKEVGWETFA 120
            WP+DN SRLG HG LEF D+VFGPESLEFD+LGRGPYTGLADGR+VRWMG+ +GWETF+
Sbjct: 61  SWPRDNLSRLGNHGKLEFVDQVFGPESLEFDSLGRGPYTGLADGRVVRWMGEAIGWETFS 120

Query: 121 IV--------CGKGVDSTTAKQWKNEKKCGRPLGLRFEKESGNLYIADAYYGLLVVGPQG 179
           +V        C +GVDSTT KQWK+EK CGRPLGLRF KE+GNLYIADAYYGLLVVGP+G
Sbjct: 121 VVTSKWSEEACVRGVDSTTNKQWKHEKLCGRPLGLRFHKETGNLYIADAYYGLLVVGPEG 180

[Close]

BLAST of Lsi05G013390 vs. Swiss-Prot
Match: SSL3_ARATH (Protein STRICTOSIDINE SYNTHASE-LIKE 3 OS=Arabidopsis thaliana GN=SSL3 PE=2 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 1.0e-27
Identity = 66/153 (43.14%), Postives = 91/153 (59.48%), Query Frame = 1


Query: 27  FVVMDPFEMGPVGGH-DFRPVKHDIAPYSQVMGRWPKDNESRLGHGNLEFEDEVFGPESL 86
           +  +DPF    +    DF+  K D+ P S +     +D ++ L +  + F +EV GPES+
Sbjct: 16  YCAIDPFSHSSISKFPDFKTYKIDMPPLSSLPKE--RDRQNLLQNSEIRFLNEVQGPESI 75

Query: 87  EFDALGRGPYTGLADGRIVRWMGKEVGWETFAIVCGKGVDSTTAKQ-----WKNEKKCGR 146
            FD  GRGPYTG+ADGRI+ W G    W  FA       +    K       K+E  CGR
Sbjct: 76  AFDPQGRGPYTGVADGRILFWNGTR--WTDFAYTSNNRSELCDPKPSLLDYLKDEDICGR 135

Query: 147 PLGLRFEKESGNLYIADAYYGLLVVGPQGGIAT 174
           PLGLRF+K++G+LYIADAY G++ VGP+GG+AT
Sbjct: 136 PLGLRFDKKNGDLYIADAYLGIMKVGPEGGLAT 164

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BLAST of Lsi05G013390 vs. Swiss-Prot
Match: SSL10_ARATH (Protein STRICTOSIDINE SYNTHASE-LIKE 10 OS=Arabidopsis thaliana GN=SSL10 PE=2 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 1.9e-26
Identity = 54/102 (52.94%), Postives = 70/102 (68.63%), Query Frame = 1


Query: 81  GPESLEFDALGRGPYTGLADGRIVRWMGKEVGWETFAIVCGKGVDSTTAKQWKNEKKCGR 140
           GPES+ FD  G GPY G++DGRI++W G+ +GW  FA       +       + E  CGR
Sbjct: 56  GPESIAFDPAGEGPYVGVSDGRILKWRGEPLGWSDFAHTSSNRQECARPFAPELEHVCGR 115

Query: 141 PLGLRFEKESGNLYIADAYYGLLVVGPQGGIATPLATHEKLQ 183
           PLGLRF+K++G+LYIADAY+GLLVVGP GG+A PL T  + Q
Sbjct: 116 PLGLRFDKKTGDLYIADAYFGLLVVGPAGGLAKPLVTEAEGQ 157

[Close]

BLAST of Lsi05G013390 vs. Swiss-Prot
Match: SSL2_ARATH (Protein STRICTOSIDINE SYNTHASE-LIKE 2 OS=Arabidopsis thaliana GN=SSL2 PE=2 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 1.2e-23
Identity = 54/101 (53.47%), Postives = 66/101 (65.35%), Query Frame = 1


Query: 81  GPESLEFDALGRGPYTGLADGRIVRWMGKEVGWETFAIVCG--KGVDSTTAKQWKNEKKC 140
           GPES  FD  G GPYTGL+DGRIV+W+  E  W  FA+     +G +     Q + E  C
Sbjct: 51  GPESFVFDFFGDGPYTGLSDGRIVKWLANESRWIDFAVTTSAREGCEGPHEHQ-RTEHVC 110

Query: 141 GRPLGLRFEKESGNLYIADAYYGLLVVGPQGGIATPLATHE 180
           GRPLGL F+K +G+LYIADAY GLL VGP GG+AT +   E
Sbjct: 111 GRPLGLAFDKSTGDLYIADAYMGLLKVGPTGGVATQVLPRE 150

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BLAST of Lsi05G013390 vs. Swiss-Prot
Match: SSL8_ARATH (Protein STRICTOSIDINE SYNTHASE-LIKE 8 OS=Arabidopsis thaliana GN=SSL8 PE=2 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 4.9e-22
Identity = 47/94 (50.00%), Postives = 62/94 (65.96%), Query Frame = 1


Query: 79  VFGPESLEFDALGRGPYTGLADGRIVRWMGKEVGWETFAIVCGKGVDSTTAKQWKNEKKC 138
           V GPESLEFD  G GPY G+ DGRI++W G+E+GW  FA               +    C
Sbjct: 52  VDGPESLEFDPQGEGPYVGVTDGRILKWRGEELGWVDFAYT---SPHRDNCSSHEVVPSC 111

Query: 139 GRPLGLRFEKESGNLYIADAYYGLLVVGPQGGIA 173
           GRPLGL FE+++G+LYI D Y+G++ VGP+GG+A
Sbjct: 112 GRPLGLSFERKTGDLYICDGYFGVMKVGPEGGLA 142

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BLAST of Lsi05G013390 vs. TrEMBL
Match: A0A0A0L9D9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G174550 PE=4 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 5.7e-94
Identity = 166/186 (89.25%), Postives = 173/186 (93.01%), Query Frame = 1


Query: 1   MEKKGVVKEESLLQHPVLFLLVLGLGFVVMDPFEMGPVGGHDFRPVKHDIAPYSQVMGRW 60
           M+KKG+VK+ESLLQHPVLFLLVLGLGFVVMDPFEM PVGG+DFRPVKHDIAPYSQVMG W
Sbjct: 1   MQKKGIVKDESLLQHPVLFLLVLGLGFVVMDPFEMSPVGGYDFRPVKHDIAPYSQVMGHW 60

Query: 61  PKDNESRLGHGNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMGKEVGWETFAI-- 120
           PKDNESRLG GNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMG+E+GWETFAI  
Sbjct: 61  PKDNESRLGLGNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMGEEIGWETFAIVT 120

Query: 121 ------VCGKGVDSTTAKQWKNEKKCGRPLGLRFEKESGNLYIADAYYGLLVVGPQGGIA 179
                 VC KGVDSTTAKQWKNEKKCGRPLGLRFEK+SGNLYIADAYYGLLVVGPQGG A
Sbjct: 121 PNWSEKVCAKGVDSTTAKQWKNEKKCGRPLGLRFEKQSGNLYIADAYYGLLVVGPQGGTA 180

[Close]

BLAST of Lsi05G013390 vs. TrEMBL
Match: M5XQY7_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa006506mg PE=4 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 1.9e-81
Identity = 144/188 (76.60%), Postives = 164/188 (87.23%), Query Frame = 1


Query: 1   MEKKGV--VKEESLLQHPVLFLLVLGLGFVVMDPFEMGPVGGHDFRPVKHDIAPYSQVMG 60
           MEKK +  +++E+LLQHP+  LLVL LGFVVMDP +MGP+G H+FRPVKH+IAPY QVM 
Sbjct: 1   MEKKKLLLLRDEALLQHPIFLLLVLVLGFVVMDPLQMGPLGDHEFRPVKHNIAPYKQVME 60

Query: 61  RWPKDNESRLGHGNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMGKEVGWETFAI 120
           RWP+DNESRLG G LEFEDEVFGPESLEFDA GRGPYTGLADGRIVRWMG ++GWETFA+
Sbjct: 61  RWPRDNESRLGFGKLEFEDEVFGPESLEFDAFGRGPYTGLADGRIVRWMGNDLGWETFAL 120

Query: 121 --------VCGKGVDSTTAKQWKNEKKCGRPLGLRFEKESGNLYIADAYYGLLVVGPQGG 179
                   VC KG+DSTT KQWK+EKKCGRPLGLRF+KESG+LYIADAYYGLLVVGPQGG
Sbjct: 121 VTSNWSKQVCAKGIDSTTHKQWKHEKKCGRPLGLRFDKESGDLYIADAYYGLLVVGPQGG 180

[Close]

BLAST of Lsi05G013390 vs. TrEMBL
Match: B9HGZ9_POPTR (Strictosidine synthase family protein OS=Populus trichocarpa GN=POPTR_0007s01770g PE=4 SV=2)

HSP 1 Score: 307.0 bits (785), Expect = 1.6e-80
Identity = 138/186 (74.19%), Postives = 162/186 (87.10%), Query Frame = 1


Query: 1   MEKKGVVKEESLLQHPVLFLLVLGLGFVVMDPFEMGPVGGHDFRPVKHDIAPYSQVMGRW 60
           MEKKG  ++ +LLQHP+L +L L +GFV+MDPF+MGP+G HDF+PVKHD+APY QVM  W
Sbjct: 1   MEKKGSQRDGTLLQHPLLLVLALAIGFVIMDPFKMGPLGHHDFKPVKHDLAPYKQVMENW 60

Query: 61  PKDNESRLGHGNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMGKEVGWETFAIV- 120
           P+DN+SRLG GNLEF +EVFGPESLEFD+LGRGPY GLADGR+VRWMG++VGWETFA+V 
Sbjct: 61  PRDNKSRLGSGNLEFVNEVFGPESLEFDSLGRGPYAGLADGRVVRWMGEDVGWETFALVS 120

Query: 121 -------CGKGVDSTTAKQWKNEKKCGRPLGLRFEKESGNLYIADAYYGLLVVGPQGGIA 179
                  C +GVDSTT+KQWK+EK CGRPLGLRF KESGNLYIADAYYGLLVVGP+GG+A
Sbjct: 121 TNWSEKLCARGVDSTTSKQWKHEKLCGRPLGLRFHKESGNLYIADAYYGLLVVGPEGGLA 180

[Close]

BLAST of Lsi05G013390 vs. TrEMBL
Match: A0A0D2S8A1_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_006G229400 PE=4 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 1.0e-79
Identity = 140/186 (75.27%), Postives = 160/186 (86.02%), Query Frame = 1


Query: 1   MEKKGVVKEESLLQHPVLFLLVLGLGFVVMDPFEMGPVGGHDFRPVKHDIAPYSQVMGRW 60
           ME+KG  KEE+ LQHPVLF++VL  GFV+MDPFE+GPVGG +FRPVKHDIAPY +VM  W
Sbjct: 1   MEQKGKHKEETFLQHPVLFIVVLVFGFVLMDPFEIGPVGGIEFRPVKHDIAPYKEVMASW 60

Query: 61  PKDNESRLGHGNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMGKEVGWETFAIV- 120
           P+DN+SRLG+G LEF DEVFGPESLEFD  GRGPYTGLADGRIVRWMG+++GWETFAIV 
Sbjct: 61  PRDNKSRLGNGKLEFVDEVFGPESLEFDGFGRGPYTGLADGRIVRWMGEDIGWETFAIVT 120

Query: 121 -------CGKGVDSTTAKQWKNEKKCGRPLGLRFEKESGNLYIADAYYGLLVVGPQGGIA 179
                  C +GVDSTT+KQWK+EK CGRPLGLRF K+ G+LYIADAYYGLLVVGP GG+A
Sbjct: 121 SNWSEKLCARGVDSTTSKQWKHEKWCGRPLGLRFNKDGGDLYIADAYYGLLVVGPGGGLA 180

[Close]

BLAST of Lsi05G013390 vs. TrEMBL
Match: B9MY61_POPTR (Strictosidine synthase family protein OS=Populus trichocarpa GN=POPTR_0017s05630g PE=4 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 5.2e-79
Identity = 136/186 (73.12%), Postives = 157/186 (84.41%), Query Frame = 1


Query: 1   MEKKGVVKEESLLQHPVLFLLVLGLGFVVMDPFEMGPVGGHDFRPVKHDIAPYSQVMGRW 60
           MEKKG  ++ +LLQHP+L +L L + FV+MDPF MGP+G HDF+P KHD+APY QVM  W
Sbjct: 1   MEKKGSQRDGTLLQHPLLLVLALAISFVIMDPFRMGPLGDHDFKPFKHDLAPYKQVMENW 60

Query: 61  PKDNESRLGHGNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMGKEVGWETFAIV- 120
           P+DN SRLG GNLEF DEVFGPESLEFD+LGRGPY GLADGR+VRWMG++VGWETFA+V 
Sbjct: 61  PRDNRSRLGSGNLEFVDEVFGPESLEFDSLGRGPYAGLADGRVVRWMGQDVGWETFALVT 120

Query: 121 -------CGKGVDSTTAKQWKNEKKCGRPLGLRFEKESGNLYIADAYYGLLVVGPQGGIA 179
                  C +GVDSTT+KQWK+EK CGRPLGLR  KESGNLYIADAYYGLLVVGP+GG+A
Sbjct: 121 TNWSEKLCARGVDSTTSKQWKHEKLCGRPLGLRLHKESGNLYIADAYYGLLVVGPEGGLA 180

[Close]

BLAST of Lsi05G013390 vs. TAIR10
Match: AT3G59530.1 (AT3G59530.1 Calcium-dependent phosphotriesterase superfamily protein)

HSP 1 Score: 279.6 bits (714), Expect = 1.4e-75
Identity = 128/189 (67.72%), Postives = 153/189 (80.95%), Query Frame = 1


Query: 1   MEKKGV--VKEESLLQHPVLFLLVLGLGFVVMDPFEMGPVGGHDFRPVKHDIAPYSQVMG 60
           MEKKG     E  +  HP+L ++ L + F+ +DPF M P+GG +F+PVKH++APY +VMG
Sbjct: 1   MEKKGQHGTYESMMTHHPILCIIALSVLFIAIDPFHMSPIGGREFKPVKHEVAPYKEVMG 60

Query: 61  RWPKDNESRLG-HGNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMGKEVGWETFA 120
            WP+DN SRLG HG LEF D+VFGPESLEFD+LGRGPYTGLADGR+VRWMG+ +GWETF+
Sbjct: 61  SWPRDNLSRLGNHGKLEFVDQVFGPESLEFDSLGRGPYTGLADGRVVRWMGEAIGWETFS 120

Query: 121 IV--------CGKGVDSTTAKQWKNEKKCGRPLGLRFEKESGNLYIADAYYGLLVVGPQG 179
           +V        C +GVDSTT KQWK+EK CGRPLGLRF KE+GNLYIADAYYGLLVVGP+G
Sbjct: 121 VVTSKWSEEACVRGVDSTTNKQWKHEKLCGRPLGLRFHKETGNLYIADAYYGLLVVGPEG 180

[Close]

BLAST of Lsi05G013390 vs. TAIR10
Match: AT5G22020.1 (AT5G22020.1 Calcium-dependent phosphotriesterase superfamily protein)

HSP 1 Score: 131.0 bits (328), Expect = 8.0e-31
Identity = 75/176 (42.61%), Postives = 105/176 (59.66%), Query Frame = 1


Query: 17  VLFLLVLGLGFVVMDPFEMGPVGGHDFRP-VKHDIAPYSQVMGRWPKDNESRLGHGNLEF 76
           + F L L L +  +DPF    +   DF   V H++         W +D+++ L    + F
Sbjct: 13  LFFSLSLAL-YCSIDPFHHCAIS--DFPNFVSHEVISPRPDEVPWERDSQNSLQKSKILF 72

Query: 77  EDEVFGPESLEFDALGRGPYTGLADGRIVRWMGKEVGWETFAIVCGKGVDSTTAKQ---- 136
            +++ GPES+ FD+LGRGPYTG+ADGR++ W G++  W  FA       +    K     
Sbjct: 73  FNQIQGPESVAFDSLGRGPYTGVADGRVLFWDGEK--WIDFAYTSSNRSEICDPKPSALS 132

Query: 137 -WKNEKKCGRPLGLRFEKESGNLYIADAYYGLLVVGPQGGIATPLATH-EKLQVGF 186
             +NE  CGRPLGLRF+K +G+LYIADAY GLL VGP+GG+ATPL T  E + +GF
Sbjct: 133 YLRNEHICGRPLGLRFDKRTGDLYIADAYMGLLKVGPEGGLATPLVTEAEGVPLGF 183

[Close]

BLAST of Lsi05G013390 vs. TAIR10
Match: AT1G08470.1 (AT1G08470.1 strictosidine synthase-like 3)

HSP 1 Score: 124.8 bits (312), Expect = 5.7e-29
Identity = 66/153 (43.14%), Postives = 91/153 (59.48%), Query Frame = 1


Query: 27  FVVMDPFEMGPVGGH-DFRPVKHDIAPYSQVMGRWPKDNESRLGHGNLEFEDEVFGPESL 86
           +  +DPF    +    DF+  K D+ P S +     +D ++ L +  + F +EV GPES+
Sbjct: 16  YCAIDPFSHSSISKFPDFKTYKIDMPPLSSLPKE--RDRQNLLQNSEIRFLNEVQGPESI 75

Query: 87  EFDALGRGPYTGLADGRIVRWMGKEVGWETFAIVCGKGVDSTTAKQ-----WKNEKKCGR 146
            FD  GRGPYTG+ADGRI+ W G    W  FA       +    K       K+E  CGR
Sbjct: 76  AFDPQGRGPYTGVADGRILFWNGTR--WTDFAYTSNNRSELCDPKPSLLDYLKDEDICGR 135

Query: 147 PLGLRFEKESGNLYIADAYYGLLVVGPQGGIAT 174
           PLGLRF+K++G+LYIADAY G++ VGP+GG+AT
Sbjct: 136 PLGLRFDKKNGDLYIADAYLGIMKVGPEGGLAT 164

[Close]

BLAST of Lsi05G013390 vs. TAIR10
Match: AT3G57030.1 (AT3G57030.1 Calcium-dependent phosphotriesterase superfamily protein)

HSP 1 Score: 120.6 bits (301), Expect = 1.1e-27
Identity = 54/102 (52.94%), Postives = 70/102 (68.63%), Query Frame = 1


Query: 81  GPESLEFDALGRGPYTGLADGRIVRWMGKEVGWETFAIVCGKGVDSTTAKQWKNEKKCGR 140
           GPES+ FD  G GPY G++DGRI++W G+ +GW  FA       +       + E  CGR
Sbjct: 56  GPESIAFDPAGEGPYVGVSDGRILKWRGEPLGWSDFAHTSSNRQECARPFAPELEHVCGR 115

Query: 141 PLGLRFEKESGNLYIADAYYGLLVVGPQGGIATPLATHEKLQ 183
           PLGLRF+K++G+LYIADAY+GLLVVGP GG+A PL T  + Q
Sbjct: 116 PLGLRFDKKTGDLYIADAYFGLLVVGPAGGLAKPLVTEAEGQ 157

[Close]

BLAST of Lsi05G013390 vs. TAIR10
Match: AT2G41290.1 (AT2G41290.1 strictosidine synthase-like 2)

HSP 1 Score: 111.3 bits (277), Expect = 6.6e-25
Identity = 54/101 (53.47%), Postives = 66/101 (65.35%), Query Frame = 1


Query: 81  GPESLEFDALGRGPYTGLADGRIVRWMGKEVGWETFAIVCG--KGVDSTTAKQWKNEKKC 140
           GPES  FD  G GPYTGL+DGRIV+W+  E  W  FA+     +G +     Q + E  C
Sbjct: 51  GPESFVFDFFGDGPYTGLSDGRIVKWLANESRWIDFAVTTSAREGCEGPHEHQ-RTEHVC 110

Query: 141 GRPLGLRFEKESGNLYIADAYYGLLVVGPQGGIATPLATHE 180
           GRPLGL F+K +G+LYIADAY GLL VGP GG+AT +   E
Sbjct: 111 GRPLGLAFDKSTGDLYIADAYMGLLKVGPTGGVATQVLPRE 150

[Close]

BLAST of Lsi05G013390 vs. NCBI nr
Match: gi|449459884|ref|XP_004147676.1| (PREDICTED: protein STRICTOSIDINE SYNTHASE-LIKE 13 [Cucumis sativus])

HSP 1 Score: 351.7 bits (901), Expect = 8.2e-94
Identity = 166/186 (89.25%), Postives = 173/186 (93.01%), Query Frame = 1


Query: 1   MEKKGVVKEESLLQHPVLFLLVLGLGFVVMDPFEMGPVGGHDFRPVKHDIAPYSQVMGRW 60
           M+KKG+VK+ESLLQHPVLFLLVLGLGFVVMDPFEM PVGG+DFRPVKHDIAPYSQVMG W
Sbjct: 1   MQKKGIVKDESLLQHPVLFLLVLGLGFVVMDPFEMSPVGGYDFRPVKHDIAPYSQVMGHW 60

Query: 61  PKDNESRLGHGNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMGKEVGWETFAI-- 120
           PKDNESRLG GNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMG+E+GWETFAI  
Sbjct: 61  PKDNESRLGLGNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMGEEIGWETFAIVT 120

Query: 121 ------VCGKGVDSTTAKQWKNEKKCGRPLGLRFEKESGNLYIADAYYGLLVVGPQGGIA 179
                 VC KGVDSTTAKQWKNEKKCGRPLGLRFEK+SGNLYIADAYYGLLVVGPQGG A
Sbjct: 121 PNWSEKVCAKGVDSTTAKQWKNEKKCGRPLGLRFEKQSGNLYIADAYYGLLVVGPQGGTA 180

[Close]

BLAST of Lsi05G013390 vs. NCBI nr
Match: gi|700202119|gb|KGN57252.1| (hypothetical protein Csa_3G174550 [Cucumis sativus])

HSP 1 Score: 351.7 bits (901), Expect = 8.2e-94
Identity = 166/186 (89.25%), Postives = 173/186 (93.01%), Query Frame = 1


Query: 1   MEKKGVVKEESLLQHPVLFLLVLGLGFVVMDPFEMGPVGGHDFRPVKHDIAPYSQVMGRW 60
           M+KKG+VK+ESLLQHPVLFLLVLGLGFVVMDPFEM PVGG+DFRPVKHDIAPYSQVMG W
Sbjct: 1   MQKKGIVKDESLLQHPVLFLLVLGLGFVVMDPFEMSPVGGYDFRPVKHDIAPYSQVMGHW 60

Query: 61  PKDNESRLGHGNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMGKEVGWETFAI-- 120
           PKDNESRLG GNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMG+E+GWETFAI  
Sbjct: 61  PKDNESRLGLGNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMGEEIGWETFAIVT 120

Query: 121 ------VCGKGVDSTTAKQWKNEKKCGRPLGLRFEKESGNLYIADAYYGLLVVGPQGGIA 179
                 VC KGVDSTTAKQWKNEKKCGRPLGLRFEK+SGNLYIADAYYGLLVVGPQGG A
Sbjct: 121 PNWSEKVCAKGVDSTTAKQWKNEKKCGRPLGLRFEKQSGNLYIADAYYGLLVVGPQGGTA 180

[Close]

BLAST of Lsi05G013390 vs. NCBI nr
Match: gi|659077181|ref|XP_008439075.1| (PREDICTED: strictosidine synthase [Cucumis melo])

HSP 1 Score: 351.3 bits (900), Expect = 1.1e-93
Identity = 166/186 (89.25%), Postives = 173/186 (93.01%), Query Frame = 1


Query: 1   MEKKGVVKEESLLQHPVLFLLVLGLGFVVMDPFEMGPVGGHDFRPVKHDIAPYSQVMGRW 60
           M+KKG+VK+ESLLQHPVLFLLVLGLGFVVMDPFEM PVGG+DFRPVKHDIAPYSQVMG W
Sbjct: 1   MKKKGIVKDESLLQHPVLFLLVLGLGFVVMDPFEMSPVGGYDFRPVKHDIAPYSQVMGHW 60

Query: 61  PKDNESRLGHGNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMGKEVGWETFAI-- 120
           PKDNESRLG GNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMG+E+GWETFAI  
Sbjct: 61  PKDNESRLGLGNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMGEEIGWETFAIVT 120

Query: 121 ------VCGKGVDSTTAKQWKNEKKCGRPLGLRFEKESGNLYIADAYYGLLVVGPQGGIA 179
                 VC KGVDSTTAKQWKNEKKCGRPLGLRFEK+SGNLYIADAYYGLLVVGPQGG A
Sbjct: 121 PNWSEKVCAKGVDSTTAKQWKNEKKCGRPLGLRFEKQSGNLYIADAYYGLLVVGPQGGTA 180

[Close]

BLAST of Lsi05G013390 vs. NCBI nr
Match: gi|645231726|ref|XP_008222532.1| (PREDICTED: strictosidine synthase 1-like [Prunus mume])

HSP 1 Score: 313.2 bits (801), Expect = 3.2e-82
Identity = 146/188 (77.66%), Postives = 166/188 (88.30%), Query Frame = 1


Query: 1   MEKKGV--VKEESLLQHPVLFLLVLGLGFVVMDPFEMGPVGGHDFRPVKHDIAPYSQVMG 60
           MEKK +  +++E+LLQHP+L LLVL LGFVVMDP +MGP+G H+FRPVKH+IAPY QVM 
Sbjct: 1   MEKKKLLLLRDEALLQHPILLLLVLVLGFVVMDPLQMGPLGDHEFRPVKHNIAPYKQVME 60

Query: 61  RWPKDNESRLGHGNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMGKEVGWETFAI 120
           RWP+DNESRLG G LEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMG ++GWETFA+
Sbjct: 61  RWPRDNESRLGFGKLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMGNDLGWETFAL 120

Query: 121 --------VCGKGVDSTTAKQWKNEKKCGRPLGLRFEKESGNLYIADAYYGLLVVGPQGG 179
                   VC KG+DSTT KQWK+EKKCGRPLGLRF+KESG+LYIADAYYGLLVVGPQGG
Sbjct: 121 VTTNWSKPVCAKGIDSTTHKQWKHEKKCGRPLGLRFDKESGDLYIADAYYGLLVVGPQGG 180

[Close]

BLAST of Lsi05G013390 vs. NCBI nr
Match: gi|596148609|ref|XP_007222749.1| (hypothetical protein PRUPE_ppa006506mg [Prunus persica])

HSP 1 Score: 310.1 bits (793), Expect = 2.7e-81
Identity = 144/188 (76.60%), Postives = 164/188 (87.23%), Query Frame = 1


Query: 1   MEKKGV--VKEESLLQHPVLFLLVLGLGFVVMDPFEMGPVGGHDFRPVKHDIAPYSQVMG 60
           MEKK +  +++E+LLQHP+  LLVL LGFVVMDP +MGP+G H+FRPVKH+IAPY QVM 
Sbjct: 1   MEKKKLLLLRDEALLQHPIFLLLVLVLGFVVMDPLQMGPLGDHEFRPVKHNIAPYKQVME 60

Query: 61  RWPKDNESRLGHGNLEFEDEVFGPESLEFDALGRGPYTGLADGRIVRWMGKEVGWETFAI 120
           RWP+DNESRLG G LEFEDEVFGPESLEFDA GRGPYTGLADGRIVRWMG ++GWETFA+
Sbjct: 61  RWPRDNESRLGFGKLEFEDEVFGPESLEFDAFGRGPYTGLADGRIVRWMGNDLGWETFAL 120

Query: 121 --------VCGKGVDSTTAKQWKNEKKCGRPLGLRFEKESGNLYIADAYYGLLVVGPQGG 179
                   VC KG+DSTT KQWK+EKKCGRPLGLRF+KESG+LYIADAYYGLLVVGPQGG
Sbjct: 121 VTSNWSKQVCAKGIDSTTHKQWKHEKKCGRPLGLRFDKESGDLYIADAYYGLLVVGPQGG 180

The following BLAST results are available for this feature:

BLAST of Lsi05G013390 vs. Swiss-Prot


Match Name	E-value	Identity	Description
SSL13_ARATH	2.5e-74	67.72	Protein STRICTOSIDINE SYNTHASE-LIKE 13 OS=Arabidopsis thaliana GN=SSL13 PE=1 SV=...	[more]
SSL3_ARATH	1.0e-27	43.14	Protein STRICTOSIDINE SYNTHASE-LIKE 3 OS=Arabidopsis thaliana GN=SSL3 PE=2 SV=1	[more]
SSL10_ARATH	1.9e-26	52.94	Protein STRICTOSIDINE SYNTHASE-LIKE 10 OS=Arabidopsis thaliana GN=SSL10 PE=2 SV=...	[more]
SSL2_ARATH	1.2e-23	53.47	Protein STRICTOSIDINE SYNTHASE-LIKE 2 OS=Arabidopsis thaliana GN=SSL2 PE=2 SV=1	[more]
SSL8_ARATH	4.9e-22	50.00	Protein STRICTOSIDINE SYNTHASE-LIKE 8 OS=Arabidopsis thaliana GN=SSL8 PE=2 SV=1	[more]

BLAST of Lsi05G013390 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L9D9_CUCSA	5.7e-94	89.25	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G174550 PE=4 SV=1	[more]
M5XQY7_PRUPE	1.9e-81	76.60	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa006506mg PE=4 SV=1	[more]
B9HGZ9_POPTR	1.6e-80	74.19	Strictosidine synthase family protein OS=Populus trichocarpa GN=POPTR_0007s01770...	[more]
A0A0D2S8A1_GOSRA	1.0e-79	75.27	Uncharacterized protein OS=Gossypium raimondii GN=B456_006G229400 PE=4 SV=1	[more]
B9MY61_POPTR	5.2e-79	73.12	Strictosidine synthase family protein OS=Populus trichocarpa GN=POPTR_0017s05630...	[more]

BLAST of Lsi05G013390 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G59530.1	1.4e-75	67.72	Calcium-dependent phosphotriesterase superfamily protein	[more]
AT5G22020.1	8.0e-31	42.61	Calcium-dependent phosphotriesterase superfamily protein	[more]
AT1G08470.1	5.7e-29	43.14	strictosidine synthase-like 3	[more]
AT3G57030.1	1.1e-27	52.94	Calcium-dependent phosphotriesterase superfamily protein	[more]
AT2G41290.1	6.6e-25	53.47	strictosidine synthase-like 2	[more]

BLAST of Lsi05G013390 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449459884\|ref\|XP_004147676.1\|	8.2e-94	89.25	PREDICTED: protein STRICTOSIDINE SYNTHASE-LIKE 13 [Cucumis sativus]	[more]
gi\|700202119\|gb\|KGN57252.1\|	8.2e-94	89.25	hypothetical protein Csa_3G174550 [Cucumis sativus]	[more]
gi\|659077181\|ref\|XP_008439075.1\|	1.1e-93	89.25	PREDICTED: strictosidine synthase [Cucumis melo]	[more]
gi\|645231726\|ref\|XP_008222532.1\|	3.2e-82	77.66	PREDICTED: strictosidine synthase 1-like [Prunus mume]	[more]
gi\|596148609\|ref\|XP_007222749.1\|	2.7e-81	76.60	hypothetical protein PRUPE_ppa006506mg [Prunus persica]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0016844	strictosidine synthase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0009058	biosynthetic process

Vocabulary: **INTERPRO**
Term	Definition
IPR011042	6-blade_b-propeller_TolB-like

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042432	indole biosynthetic process
biological_process	GO:0010584	pollen exine formation
biological_process	GO:0016114	terpenoid biosynthetic process
biological_process	GO:0009827	plant-type cell wall modification
biological_process	GO:0009860	pollen tube growth
biological_process	GO:0009058	biosynthetic process
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds
molecular_function	GO:0016844	strictosidine synthase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G013390.1	Lsi05G013390.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011042	Six-bladed beta-propeller, TolB-like	GENE3D	G3DSA:2.120.10.30		coord: 75..179 score: 1.0
None	No IPR available	PANTHER	PTHR10426:SF21	POLLEN DEVELOPMENT PROTEIN LAP3	coord: 8..178 score: 3.3E
None	No IPR available	unknown	SSF63829	Calcium-dependent phosphotriesterase	coord: 74..106 score: 3.53E-10coord: 138..179 score: 3.53

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi05G013390	Csa3G174550	Cucumber (Chinese Long) v2	culsiB210
Lsi05G013390	CSPI03G15790	Wild cucumber (PI 183967)	cpilsiB208
Lsi05G013390	Cucsa.251750	Cucumber (Gy14) v1	cgylsiB416
Lsi05G013390	CmaCh14G015160	Cucurbita maxima (Rimu)	cmalsiB246
Lsi05G013390	CmoCh14G015500	Cucurbita moschata (Rifu)	cmolsiB234
Lsi05G013390	Cla021877	Watermelon (97103) v1	lsiwmB366
Lsi05G013390	MELO3C006800	Melon (DHL92) v3.5.1	lsimeB379
Lsi05G013390	ClCG05G007240	Watermelon (Charleston Gray)	lsiwcgB336
Lsi05G013390	MELO3C006800.2	Melon (DHL92) v3.6.1	lsimedB397
Lsi05G013390	Cla97C05G088990	Watermelon (97103) v2	lsiwmbB334
Lsi05G013390	Bhi01G000880	Wax gourd	lsiwgoB444
Lsi05G013390	Cp4.1LG03g12400	Cucurbita pepo (Zucchini)	cpelsiB507
Lsi05G013390	CsGy3G015880	Cucumber (Gy14) v2	cgyblsiB197
Lsi05G013390	Carg24799	Silver-seed gourd	carlsiB951
Lsi05G013390	CsaV3_3G016000	Cucumber (Chinese Long) v3	cuclsiB229

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi05G013390	Silver-seed gourd	carlsiB633
Lsi05G013390	Cucumber (Chinese Long) v3	cuclsiB245
Lsi05G013390	Cucurbita maxima (Rimu)	cmalsiB765
Lsi05G013390	Cucurbita moschata (Rifu)	cmolsiB759
Lsi05G013390	Cucurbita pepo (Zucchini)	cpelsiB715