Lsi05G012210 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi05G012210
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Biotin/lipoate A/B protein ligase family
Location	chr05 : 20107307 .. 20109727 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGGTAAGTTCTGTGAATCGTATAAGAAGTCTCAGCGTCAGAGGTCCCTTTCGGAGCAGGGTTTCGCAAACTTTCTGTCAATGCACAAACAATACTTATGAACAACAATAATACAAAGAAGAAGAAAAGGCTGATAATTTCTGAAGCAAAGATTAAAAAAGAAAAGCATACAGCGACGAGATTTCCGAAGTGTGCAGTGATAATGTGGACGGCATATTTGATGCCGTACTGAGTCATCACCATGATTGCCTTCTTTAAGATTGCTGTGCGTCTATGACATCAAGTATCCTACAGTACCAACCAACAATTTATAGTTAAATATACCAAAATAACAATAAAATAATACAATAAATAAAAAAATTGACCCTTAGTGACTTTTGCATTCTCTCGCTATGTCTTGCACTTGCAAACCAATGTGAGTAAGGTCACAAACAAAGTATCAATCAAATATCTTAGAACAACGAACAACTCACATTTTGTTCATCACACAACAATGTAAAATAGAAGCAATTTTTTCCTCAAGAAAAAGTATAAAACAACGGATGAAAGTTTCTCAGCAAGCCTACACAAAGAGAAATATTTCAGAAGCTGTGGCTGCCATTGAAGATTTCATAAAGAAGGAATATCACAGGAGCTGCCGTGCGTGGAGAGATGAACGTCATCACGTCGCCAAGGTTTCAGCGGCTCAAAGAGTAGCCGCGCGTGCGAGAGAGAGGAACGAAGGAAGTGGTAATTAGGGAAATTTTTTCTTTTTTTCTCTTACCAAAAAATTAGGGTTTTTCTAAAGAGGCAAAATTGATCGTTCATCACCTCAAATTCCAATTACATTACCAAACAGCATGAGGATATCAAAGAAATGGCAAGATATTAAATCTTAGTTAGGCTGTCACTTTAAATTAAACTCACTCTTAAAGGGCCTTTTATTAAACCCATGATGATTTAGTATATATAATTATATGTTTAGTCTTTAAGCTTTTAAGTTAATATCTATTCACCAAAGCGAGCATAGCTCACATATAGAATATGTTAGTGTTAGACCCCCAATTTTATATACTTATAAGAGGAGGGGTAGGACAGGAAAAACACAAGGAAAAGGAGGGGATTGGAAAGAGAGGAGGCACATATTTTCGGTTAGAGGAAGGGACCACTTTGTACGATCTTAAAAAGGGAAAAAGACGTGAGAAGAAGGTGTGAGAAAATTGAAGTACAAGAGGAGGCCCTTTCAGGGAAAAGGAGAGAATTCCTCAGCTCTCTCTAAAGTGTTGGGTTGTCATTCCATTTCAGCTTTTCTTGATATTGTACTGTTTTCCATTTTTGTGTTTCTTGATCCTATTTTGGGATTGTTGAACTTCTGGGCATTATTCCTTGGTTAATATATAAGGGATCAAGCATTATTACTCTATCTCTTGTCTATATATCCTTTGCTTGTTGGGTTTTCTGCGTGCAGGGATAACAGAACCTAACAAATTGGTATCAGAGTGCTTAAACCTAGGGCGAACACAGAAGATGGCAGCAAATGGAGGAGAAGATGGAAGCCAATGAGAAAGAAATCTCCAATCTGAAGGAAATGATATTCGGTTTATGTAAAAATATTGAACTAAAGTTTGAAGAGGTGGTGAGAGCTCGATGAATAGAAAGTTGGGAGAATCGAGGAGACAGAGGTGGAACTCGCCGAGGAGACGGAGGTAGAAAAATTGAAGGTCGTGGAACTAGAGGAGAACGTGCATTGAAGATCATTCTTAGTCTCCACCATCTACATTCGACCGGTTCTTTAAGTAACAGTTTTCTGTAAAGCCACTCACTTGCTCAAAATTAGATTATTCTTCGTGCACAATCATCCAAAATCGATTTGTTTGTTGCTGGAAATCTAGCGATCTATATTTCTCTCAGCTAATAGATTTGGTCTGGTTTTCGAGAATTTCTAGATTGGATATGTTTTTCCGGTTTGCTGGGAGTATTTTTCTAATATGATGATGTGGTTACTACAAACTCAATCTGCTCTTGTTTGAGTTATGGCAACTTAATTGAAGTCGACTTCTTTTTTGGAAGTAGGAGAAGAAAAGGGATCTTATTATCAAAAATTTCTCTTTCCAACGACGATGTATCAAACAAGAAATATTGGACATTCTTTGATCAATCTTGTGAGGTTGAAGGTTTTCCTATTCTGCAGCAACTACAATTGGAAGAACGCCTACTTCGAAACTCATCAGATAATTGGTGTATCATAAATGATGGAACAGATAATCCAACTGTTGTGATGGGCATTCCTAGGAAACCTGATGAGCTCCTTGACATCAAATCTGTGTTAGGAGATCAAATTCCAGTCATCAGAAGGTTCACAGGGGGGGAACTGTTGTTGTTGATCACAACACAGTATTTGTCACCTTCATATGTAATAAAGATGTTGTCGCTAAACTAA

mRNA sequence

ATGAGAAAAAGTATAAAACAACGGATGAAAGTTTCTCAGCAAGCCTACACAAAGAGAAATATTTCAGAAGCTGTGGCTGCCATTGAAGATTTCATAAAGAAGGAATATCACAGGAGCTGCCGTGCGTGGAGAGATGAACGTCATCACGTCGCCAAGGTTTCAGCGGCTCAAAGAGTAGCCGCGCGTGCGAGAGAGAGGAACGAAGGAAGTGGTTTTCCTATTCTGCAGCAACTACAATTGGAAGAACGCCTACTTCGAAACTCATCAGATAATTGGTGTATCATAAATGATGGAACAGATAATCCAACTGTTGTGATGGGCATTCCTAGGAAACCTGATGAGCTCCTTGACATCAAATCTGTGTTAGGAGATCAAATTCCAGTCATCAGAAGGTTCACAGGGGGGGAACTGTTGTTGTTGATCACAACACAGTATTTGTCACCTTCATATGTAATAAAGATGTTGTCGCTAAACTAA

Coding sequence (CDS)

ATGAGAAAAAGTATAAAACAACGGATGAAAGTTTCTCAGCAAGCCTACACAAAGAGAAATATTTCAGAAGCTGTGGCTGCCATTGAAGATTTCATAAAGAAGGAATATCACAGGAGCTGCCGTGCGTGGAGAGATGAACGTCATCACGTCGCCAAGGTTTCAGCGGCTCAAAGAGTAGCCGCGCGTGCGAGAGAGAGGAACGAAGGAAGTGGTTTTCCTATTCTGCAGCAACTACAATTGGAAGAACGCCTACTTCGAAACTCATCAGATAATTGGTGTATCATAAATGATGGAACAGATAATCCAACTGTTGTGATGGGCATTCCTAGGAAACCTGATGAGCTCCTTGACATCAAATCTGTGTTAGGAGATCAAATTCCAGTCATCAGAAGGTTCACAGGGGGGGAACTGTTGTTGTTGATCACAACACAGTATTTGTCACCTTCATATGTAATAAAGATGTTGTCGCTAAACTAA

Protein sequence

MRKSIKQRMKVSQQAYTKRNISEAVAAIEDFIKKEYHRSCRAWRDERHHVAKVSAAQRVAARARERNEGSGFPILQQLQLEERLLRNSSDNWCIINDGTDNPTVVMGIPRKPDELLDIKSVLGDQIPVIRRFTGGELLLLITTQYLSPSYVIKMLSLN

Homology

[Close]

BLAST of Lsi05G012210 vs. TrEMBL
Match: A0A0A0LSR7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G033280 PE=4 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 1.1e-26
Identity = 59/70 (84.29%), Postives = 65/70 (92.86%), Query Frame = 1


Query: 71  GFPILQQLQLEERLLRNSSDNWCIINDGTDNPTVVMGIPRKPDELLDIKSVLGDQIPVIR 130
           GFPILQQL+LEERLLRNSSDNWCIINDGTDNP +VMG+  KPDELLDIKSVLGDQIPVIR
Sbjct: 21  GFPILQQLKLEERLLRNSSDNWCIINDGTDNPAIVMGVSGKPDELLDIKSVLGDQIPVIR 80

Query: 131 RFTGGELLLL 141
           RFTGG  +++
Sbjct: 81  RFTGGGTVIV 90

[Close]

BLAST of Lsi05G012210 vs. TrEMBL
Match: A0A0B0PAB8_GOSAR (Putative lipoate-protein ligase A OS=Gossypium arboreum GN=F383_06916 PE=4 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 1.2e-20
Identity = 48/70 (68.57%), Postives = 61/70 (87.14%), Query Frame = 1


Query: 71  GFPILQQLQLEERLLRNSSDNWCIINDGTDNPTVVMGIPRKPDELLDIKSVLGDQIPVIR 130
           G PILQQL +EE+LLR SS+NWCI+NDGT++PTVVMG+  KP ELL+I+SVL DQ+PVIR
Sbjct: 21  GLPILQQLHIEEQLLRTSSENWCIVNDGTNDPTVVMGVSGKPAELLEIESVLDDQVPVIR 80

Query: 131 RFTGGELLLL 141
           RFTGG  +++
Sbjct: 81  RFTGGGTVIV 90

[Close]

BLAST of Lsi05G012210 vs. TrEMBL
Match: A0A061FXT9_THECC (Biotin/lipoate A/B protein ligase family OS=Theobroma cacao GN=TCM_014047 PE=4 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 1.6e-20
Identity = 48/70 (68.57%), Postives = 61/70 (87.14%), Query Frame = 1


Query: 71  GFPILQQLQLEERLLRNSSDNWCIINDGTDNPTVVMGIPRKPDELLDIKSVLGDQIPVIR 130
           G PILQQL +EE+LLR SS+NWCIINDGT++PT+VMG+  KP ELL+I+SVL DQ+PVIR
Sbjct: 21  GLPILQQLHIEEQLLRTSSENWCIINDGTNDPTIVMGVSGKPAELLEIESVLRDQVPVIR 80

Query: 131 RFTGGELLLL 141
           RFTGG  +++
Sbjct: 81  RFTGGGTVIV 90

[Close]

BLAST of Lsi05G012210 vs. TrEMBL
Match: A0A0J8CT74_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_3g063730 PE=4 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 1.6e-20
Identity = 50/71 (70.42%), Postives = 61/71 (85.92%), Query Frame = 1


Query: 70  SGFPILQQLQLEERLLRNSSDNWCIINDGTDNPTVVMGIPRKPDELLDIKSVLGDQIPVI 129
           +G PILQQL LEE+LLR SS+NWCIINDGT +PT+VMGI  KP ELLD+KSVL D+IPVI
Sbjct: 7   NGVPILQQLHLEEKLLRISSENWCIINDGTPDPTIVMGISGKPAELLDVKSVLQDKIPVI 66

Query: 130 RRFTGGELLLL 141
           +RFTGG  +++
Sbjct: 67  KRFTGGGTVIV 77

[Close]

BLAST of Lsi05G012210 vs. TrEMBL
Match: A0A0K9RFH4_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_072770 PE=4 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 2.0e-20
Identity = 50/71 (70.42%), Postives = 60/71 (84.51%), Query Frame = 1


Query: 70  SGFPILQQLQLEERLLRNSSDNWCIINDGTDNPTVVMGIPRKPDELLDIKSVLGDQIPVI 129
           +G PILQQLQLEE+LLR SSDNWCIINDGT +P +VMGI  KP EL+D+KSVL D+I VI
Sbjct: 7   NGVPILQQLQLEEKLLRTSSDNWCIINDGTPDPAIVMGISGKPAELVDVKSVLRDKISVI 66

Query: 130 RRFTGGELLLL 141
           RRFTGG  +++
Sbjct: 67  RRFTGGGTVIV 77

[Close]

BLAST of Lsi05G012210 vs. TAIR10
Match: AT3G29010.1 (AT3G29010.1 Biotin/lipoate A/B protein ligase family)

HSP 1 Score: 99.8 bits (247), Expect = 1.6e-21
Identity = 44/70 (62.86%), Postives = 58/70 (82.86%), Query Frame = 1


Query: 71  GFPILQQLQLEERLLRNSSDNWCIINDGTDNPTVVMGIPRKPDELLDIKSVLGDQIPVIR 130
           G PIL+QL LEERLLR SSDNWCI+NDGT+ PT+VMG+  KP +LL++  V+ D+IPVI+
Sbjct: 21  GTPILEQLHLEERLLRTSSDNWCIVNDGTNVPTIVMGMSGKPSQLLEVGPVMEDRIPVIK 80

Query: 131 RFTGGELLLL 141
           RFTGG  +++
Sbjct: 81  RFTGGGTVIV 90

[Close]

BLAST of Lsi05G012210 vs. NCBI nr
Match: gi|659066404|ref|XP_008443042.1| (PREDICTED: putative lipoate-protein ligase A [Cucumis melo])

HSP 1 Score: 129.4 bits (324), Expect = 5.5e-27
Identity = 60/70 (85.71%), Postives = 65/70 (92.86%), Query Frame = 1


Query: 71  GFPILQQLQLEERLLRNSSDNWCIINDGTDNPTVVMGIPRKPDELLDIKSVLGDQIPVIR 130
           GFPILQQL LEERLLRNSSDNWCIINDGTDNPT+VMG+  KPDELLDIKSVLGDQIPVIR
Sbjct: 21  GFPILQQLHLEERLLRNSSDNWCIINDGTDNPTIVMGVSGKPDELLDIKSVLGDQIPVIR 80

Query: 131 RFTGGELLLL 141
           RFTGG  +++
Sbjct: 81  RFTGGGTVIV 90

[Close]

BLAST of Lsi05G012210 vs. NCBI nr
Match: gi|449439277|ref|XP_004137412.1| (PREDICTED: putative lipoate-protein ligase A [Cucumis sativus])

HSP 1 Score: 127.9 bits (320), Expect = 1.6e-26
Identity = 59/70 (84.29%), Postives = 65/70 (92.86%), Query Frame = 1


Query: 71  GFPILQQLQLEERLLRNSSDNWCIINDGTDNPTVVMGIPRKPDELLDIKSVLGDQIPVIR 130
           GFPILQQL+LEERLLRNSSDNWCIINDGTDNP +VMG+  KPDELLDIKSVLGDQIPVIR
Sbjct: 21  GFPILQQLKLEERLLRNSSDNWCIINDGTDNPAIVMGVSGKPDELLDIKSVLGDQIPVIR 80

Query: 131 RFTGGELLLL 141
           RFTGG  +++
Sbjct: 81  RFTGGGTVIV 90

[Close]

BLAST of Lsi05G012210 vs. NCBI nr
Match: gi|728841801|gb|KHG21244.1| (putative lipoate-protein ligase A [Gossypium arboreum])

HSP 1 Score: 107.8 bits (268), Expect = 1.7e-20
Identity = 48/70 (68.57%), Postives = 61/70 (87.14%), Query Frame = 1


Query: 71  GFPILQQLQLEERLLRNSSDNWCIINDGTDNPTVVMGIPRKPDELLDIKSVLGDQIPVIR 130
           G PILQQL +EE+LLR SS+NWCI+NDGT++PTVVMG+  KP ELL+I+SVL DQ+PVIR
Sbjct: 21  GLPILQQLHIEEQLLRTSSENWCIVNDGTNDPTVVMGVSGKPAELLEIESVLDDQVPVIR 80

Query: 131 RFTGGELLLL 141
           RFTGG  +++
Sbjct: 81  RFTGGGTVIV 90

[Close]

BLAST of Lsi05G012210 vs. NCBI nr
Match: gi|870864131|gb|KMT15264.1| (hypothetical protein BVRB_3g063730 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 107.5 bits (267), Expect = 2.2e-20
Identity = 50/71 (70.42%), Postives = 61/71 (85.92%), Query Frame = 1


Query: 70  SGFPILQQLQLEERLLRNSSDNWCIINDGTDNPTVVMGIPRKPDELLDIKSVLGDQIPVI 129
           +G PILQQL LEE+LLR SS+NWCIINDGT +PT+VMGI  KP ELLD+KSVL D+IPVI
Sbjct: 7   NGVPILQQLHLEEKLLRISSENWCIINDGTPDPTIVMGISGKPAELLDVKSVLQDKIPVI 66

Query: 130 RRFTGGELLLL 141
           +RFTGG  +++
Sbjct: 67  KRFTGGGTVIV 77

[Close]

BLAST of Lsi05G012210 vs. NCBI nr
Match: gi|823122222|ref|XP_012469970.1| (PREDICTED: putative lipoate-protein ligase A isoform X2 [Gossypium raimondii])

HSP 1 Score: 107.5 bits (267), Expect = 2.2e-20
Identity = 47/70 (67.14%), Postives = 61/70 (87.14%), Query Frame = 1


Query: 71  GFPILQQLQLEERLLRNSSDNWCIINDGTDNPTVVMGIPRKPDELLDIKSVLGDQIPVIR 130
           G PILQQL +EE+LLR SS+NWCI+NDGT++PT+VMG+  KP ELL+I+SVL DQ+PVIR
Sbjct: 21  GLPILQQLHIEEQLLRTSSENWCIVNDGTNDPTIVMGVSGKPAELLEIESVLDDQVPVIR 80

Query: 131 RFTGGELLLL 141
           RFTGG  +++
Sbjct: 81  RFTGGGTVIV 90

The following BLAST results are available for this feature:

BLAST of Lsi05G012210 vs. Swiss-Prot


Match Name	E-value	Identity	Description

BLAST of Lsi05G012210 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LSR7_CUCSA	1.1e-26	84.29	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G033280 PE=4 SV=1	[more]
A0A0B0PAB8_GOSAR	1.2e-20	68.57	Putative lipoate-protein ligase A OS=Gossypium arboreum GN=F383_06916 PE=4 SV=1	[more]
A0A061FXT9_THECC	1.6e-20	68.57	Biotin/lipoate A/B protein ligase family OS=Theobroma cacao GN=TCM_014047 PE=4 S...	[more]
A0A0J8CT74_BETVU	1.6e-20	70.42	Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_3g063730 PE=4 S...	[more]
A0A0K9RFH4_SPIOL	2.0e-20	70.42	Uncharacterized protein OS=Spinacia oleracea GN=SOVF_072770 PE=4 SV=1	[more]

BLAST of Lsi05G012210 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G29010.1	1.6e-21	62.86	Biotin/lipoate A/B protein ligase family	[more]

BLAST of Lsi05G012210 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659066404\|ref\|XP_008443042.1\|	5.5e-27	85.71	PREDICTED: putative lipoate-protein ligase A [Cucumis melo]	[more]
gi\|449439277\|ref\|XP_004137412.1\|	1.6e-26	84.29	PREDICTED: putative lipoate-protein ligase A [Cucumis sativus]	[more]
gi\|728841801\|gb\|KHG21244.1\|	1.7e-20	68.57	putative lipoate-protein ligase A [Gossypium arboreum]	[more]
gi\|870864131\|gb\|KMT15264.1\|	2.2e-20	70.42	hypothetical protein BVRB_3g063730 [Beta vulgaris subsp. vulgaris]	[more]
gi\|823122222\|ref\|XP_012469970.1\|	2.2e-20	67.14	PREDICTED: putative lipoate-protein ligase A isoform X2 [Gossypium raimondii]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006464	cellular protein modification process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016874	ligase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G012210.1	Lsi05G012210.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	G3DSA:3.90.1550.10		coord: 72..136 score: 1.0
None	No IPR available	PANTHER	PTHR12561	LIPOATE-PROTEIN LIGASE	coord: 70..135 score: 5.7
None	No IPR available	PANTHER	PTHR12561:SF9	BIOTIN/LIPOATE A/B PROTEIN LIGASE FAMILY PROTEIN	coord: 70..135 score: 5.7
None	No IPR available	unknown	SSF55681	Class II aaRS and biotin synthetases	coord: 72..138 score: 4.31

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi05G012210	Wax gourd	lsiwgoB434
Lsi05G012210	Wax gourd	lsiwgoB443
Lsi05G012210	Bottle gourd (USVL1VR-Ls)	lsilsiB160
Lsi05G012210	Cucumber (Gy14) v1	cgylsiB143
Lsi05G012210	Cucurbita maxima (Rimu)	cmalsiB312
Lsi05G012210	Cucurbita maxima (Rimu)	cmalsiB387
Lsi05G012210	Cucurbita maxima (Rimu)	cmalsiB587
Lsi05G012210	Cucurbita maxima (Rimu)	cmalsiB836
Lsi05G012210	Cucurbita moschata (Rifu)	cmolsiB300
Lsi05G012210	Cucurbita moschata (Rifu)	cmolsiB372
Lsi05G012210	Cucurbita moschata (Rifu)	cmolsiB567
Lsi05G012210	Cucurbita pepo (Zucchini)	cpelsiB035
Lsi05G012210	Cucurbita pepo (Zucchini)	cpelsiB172
Lsi05G012210	Cucurbita pepo (Zucchini)	cpelsiB618
Lsi05G012210	Wild cucumber (PI 183967)	cpilsiB208
Lsi05G012210	Cucumber (Chinese Long) v2	culsiB210
Lsi05G012210	Melon (DHL92) v3.5.1	lsimeB379
Lsi05G012210	Watermelon (Charleston Gray)	lsiwcgB336
Lsi05G012210	Watermelon (97103) v1	lsiwmB366
Lsi05G012210	Cucumber (Gy14) v2	cgyblsiB193
Lsi05G012210	Cucumber (Gy14) v2	cgyblsiB197
Lsi05G012210	Melon (DHL92) v3.6.1	lsimedB394
Lsi05G012210	Melon (DHL92) v3.6.1	lsimedB397
Lsi05G012210	Silver-seed gourd	carlsiB650
Lsi05G012210	Silver-seed gourd	carlsiB675
Lsi05G012210	Silver-seed gourd	carlsiB903
Lsi05G012210	Cucumber (Chinese Long) v3	cuclsiB229
Lsi05G012210	Watermelon (97103) v2	lsiwmbB331
Lsi05G012210	Watermelon (97103) v2	lsiwmbB334