Lsi05G012190 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi05G012190
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Cytochrome P450
Location	chr05 : 20089302 .. 20092696 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAATGGAGTTGATTTGTGGGTTGATTATGTTAATCATGTGGGTGATTAGGGTTTCAATTATGGTTTTATTTGTGAAAGTGGTGGTTTCATGGTGGATTTTGCCTATTTTGGCTCATAAAAAATTGAAGAGAAATGGATTTGAAGGTCCTTCTCCTACTTTCCCTCTTGGAAACATTACAGAAATGAAGAAAATAATGAAAGCTGCTGCAGCCATTAATGGATCATCAACTACCTCCCTTGATGCTTCTTCAAATAATGTCTCCCATGATATTCATTCAACTATTTTCCCTCACTTTGCTCAATGGCAGAATTCTTATGGTAATCAAACAAAACCCTTCTTTCCTTATATAATATATATGTTGTTTTTTTAAATATATAGTAATCGTCGTCGTTGTTACTTTTTATGATTGTTCAATGCAAGAACGATACTACAGTGCATCCTAGTCTACACCTATCTTTATGCATCTCACTAAATTGCTCAATCATAATCTTCCATGTGGCAAATGTTTTTTAATTCTTTTTTTGTGTGTATGATGAGAATTGGATGCATAAAGATGTGTATAGATGCATTCATGTATTGCTATCATCCAATTATATACTAAGTTTCTAATGTGGCATATTTTTATTGATATTGTTTTCGTCGTATTAAATAAATAAAAGCCAGCAAAATGTCACTTAAATATGTGTGTGCACGCGCACACATACAAACTCGTGCATAGTATTATGTTATTAGTCATCTTTAAATTTTGAAAAAAATTTAGGCGTTGTCTAATGTGCATGTCAGATAGTTATCCAATAAAAATTTGTCATATGCTATTTTTTTTTAATTGAGTTATGTTTTTCTTTTTTTGTTTTTACTATGTACCAAAAGAAAGATGCATAGAATTAGGTGCAATCTAGACTACACCTAATCGTTACTCTTACAATTTTAGCGTAGGTTATTTTTGAGCTAATTTTAATATTATAGTGCAGTTGGAAATTTGAATGCTAAGTTTTTCAATATTAATGAATAGTAATGAGAAAATTAGAATGTAGCTTAAGAATGATTTTGTGTTATTACAACAGGGAAGAAGTTTGTATATTGGTTGGGGACAGAGCCATTTTTGTACATAGCAGATCCACAGCTTGTGAAGAAGATATCTGAAGCTGTAATGGGGAAGAGTTGGGGGAAGCCAACTGTCTTCCGAAACGATCGAGAACCGATGTTCGGCGATGGCCTCGTCATGACCGAAGGCGACGACTGGATTCGTCATCGCCACATCCTCACTCCCGCCTTCAACCCCACCAATTTAAAGGTAATTTAACTTACATGTTTCACTCTTAATTTAAATTTTAAACAGCATTTTGTTTAAATTCTAAATTCAAACTTGTTAAACTTAAAGTTGTAGTAAAATGATTATGAATAATCTAACATGAAGTTAGGTAATAAGAAAGATTTTTTTATTGTTATTATTGTTATTATCGTTAAGATTATTATATATATTTTTTTCGGCAAAAGAAGATTTTTCTTTGTTTATTAATATATATGTGAAAACTGACACGCAACCATTGGTGGTGGAGATATGACATCATTCTATTTGGATCAACAATATCTTATCTTTAATGACCAAATAAATTATATGATTAGTTGATGGTTTGGTTGATAAGATTGATTTGTCTCTTTCTCCTAATAAAATGTTATATATTAAAAAACAAATTTTGTACTTTAAAAAAAAAAAAAAAACAAAATTTTGAGTTTAATTTTTCTTTAATTTATAATTTTCAAAATATTAAAACATTACTTTGACTTGAAAGTTTAACAACTTTCATGTGGTTCTCATTTTCACCCTTTAATTTCAATTTTGTTTTTTAGGTTTCAATATTTTATGATTATATTATCTAATCTTATTAATAAATTAAAGTTTATTTTTTATTTTTTAAACTAACTTTTTATTAATTAATTTAAAATAATTATGATGTGAAAATTTTTAATTAATTCTAATCGAAGCAAAAAATTAGTGAAAACTAATTATAATTAACCTCAAAGTTGATATCTAGTGAAAAACTAAAAGGGTAAATCTTTTGAAACTCAAATAATGAAAACAAAAAAAACATAAGTAAAAGTATAACCATTGAAAACTAAGACAAAAATTAAAGACTATACTAAAAGGTACTCATGCATCAAAATATATATATATATAATTTCTCTTGCATAAACACATGCATTATTATTGATATGATCTCGTTAAAAAAATCGTGAATGTTATATAAAATCTTATTTAAATAAAAAAAATAGTATTAATAAAAAAAGAGCAATAAGGATGTAATTTGAGAGGATGTATAACATAACCAAGGTTTAAAATATCGATATCAATAGAAATATCGAGGTCTTAATTTTACACAAATTTCAATGGAAATAATATCGATAAAATATCGATTTCGATAGATATTTCTAGAAAAATTATAAAAATAAAAAAATAAATTTAAATTAGTAAATAAATATTTTTATTATTTTCAAATAAGTAAACATGTCTATTATTTATATTAGTGACATTTTGATGTTTCGTAAATTTTTGTACTGACATAATGAAAATATCAATTCACCCATACTATCGATATTGAACCTACGAAAACATAGAAATATAAATTTCGACATATCGATGAAAATTTAGTACTATGAAGGTAACAATATAAGTTTTTTAACTATAAATAATAATGGACATAAAAATAATAATAAATAATTAATTTAAATATCAGGCAATGACAAAGTTCATGGTGGAGTCCACCACAAAAATGGTAAAAGAGTGGAGAAGCCTGGTAAAGTCGGGGAAACCGGAAATCGACGTGGAGAGAGAAATCACGGCAACGGCCGGGGACATAATCGCAAAGGCAAGCTTCGGAATTTGGAGTGGCGGCGGCGGTGAAGAGGCATTGAATAACCTGAGAGCTCTTCAATTGACTCTGTTCTCGAACAGCAGCTACGTGGGGGTCCCATTCAGCAGTATATTTCTGTACCCTGCTCGAACCGTAGCCGCCAAGAGACTCGGTGCCCAAATCGACCGCCACTTCTTTTCCATCATATCTCACAGGAAACTCTCTTCCGATTCCGATTCCGATTTGCTGAGTCGGATGATTCAGAGTCAAAGCAACCAGAAGCAGGGCAGCGCCGCCCAGCTGACCCCTAGAGAAGTGGTGGACGAGTGTAAGACTTTCTTCTTCGGTGGCCATGAGACGACGGCGCTTGCCATCAGTTGGACGCTGCTGCTGCTGGCCACGCGCCCCGATTGGCAAAACATTTTACGAGAGGAGATTAAAGAAGTTATCGGAGATAAAGATATTGATTTCTCCATGCTTTCTTCACTCAAAAAGGTTTCTCCATATTTTCTCTTTTTCCTATTTGCTCTGCTCATATGA

mRNA sequence

AAAAATGGAGTTGATTTGTGGGTTGATTATGTTAATCATGTGGGTGATTAGGGTTTCAATTATGGTTTTATTTGTGAAAGTGGTGGTTTCATGGTGGATTTTGCCTATTTTGGCTCATAAAAAATTGAAGAGAAATGGATTTGAAGGTCCTTCTCCTACTTTCCCTCTTGGAAACATTACAGAAATGAAGAAAATAATGAAAGCTGCTGCAGCCATTAATGGATCATCAACTACCTCCCTTGATGCTTCTTCAAATAATGTCTCCCATGATATTCATTCAACTATTTTCCCTCACTTTGCTCAATGGCAGAATTCTTATGGGAAGAAGTTTGTATATTGGTTGGGGACAGAGCCATTTTTGTACATAGCAGATCCACAGCTTGTGAAGAAGATATCTGAAGCTGTAATGGGGAAGAGTTGGGGGAAGCCAACTGTCTTCCGAAACGATCGAGAACCGATGTTCGGCGATGGCCTCGTCATGACCGAAGGCGACGACTGGATTCGTCATCGCCACATCCTCACTCCCGCCTTCAACCCCACCAATTTAAAGGCAATGACAAAGTTCATGGTGGAGTCCACCACAAAAATGGTAAAAGAGTGGAGAAGCCTGGTAAAGTCGGGGAAACCGGAAATCGACGTGGAGAGAGAAATCACGGCAACGGCCGGGGACATAATCGCAAAGGCAAGCTTCGGAATTTGGAGTGGCGGCGGCGGTGAAGAGGCATTGAATAACCTGAGAGCTCTTCAATTGACTCTGTTCTCGAACAGCAGCTACGTGGGGGTCCCATTCAGCAGTATATTTCTGTACCCTGCTCGAACCGTAGCCGCCAAGAGACTCGGTGCCCAAATCGACCGCCACTTCTTTTCCATCATATCTCACAGGAAACTCTCTTCCGATTCCGATTCCGATTTGCTGAGTCGGATGATTCAGAGTCAAAGCAACCAGAAGCAGGGCAGCGCCGCCCAGCTGACCCCTAGAGAAGTGGTGGACGAGTGTAAGACTTTCTTCTTCGGTGGCCATGAGACGACGGCGCTTGCCATCAGTTGGACGCTGCTGCTGCTGGCCACGCGCCCCGATTGGCAAAACATTTTACGAGAGGAGATTAAAGAAGTTATCGGAGATAAAGATATTGATTTCTCCATGCTTTCTTCACTCAAAAAGGTTTCTCCATATTTTCTCTTTTTCCTATTTGCTCTGCTCATATGA

Coding sequence (CDS)

ATGGAGTTGATTTGTGGGTTGATTATGTTAATCATGTGGGTGATTAGGGTTTCAATTATGGTTTTATTTGTGAAAGTGGTGGTTTCATGGTGGATTTTGCCTATTTTGGCTCATAAAAAATTGAAGAGAAATGGATTTGAAGGTCCTTCTCCTACTTTCCCTCTTGGAAACATTACAGAAATGAAGAAAATAATGAAAGCTGCTGCAGCCATTAATGGATCATCAACTACCTCCCTTGATGCTTCTTCAAATAATGTCTCCCATGATATTCATTCAACTATTTTCCCTCACTTTGCTCAATGGCAGAATTCTTATGGGAAGAAGTTTGTATATTGGTTGGGGACAGAGCCATTTTTGTACATAGCAGATCCACAGCTTGTGAAGAAGATATCTGAAGCTGTAATGGGGAAGAGTTGGGGGAAGCCAACTGTCTTCCGAAACGATCGAGAACCGATGTTCGGCGATGGCCTCGTCATGACCGAAGGCGACGACTGGATTCGTCATCGCCACATCCTCACTCCCGCCTTCAACCCCACCAATTTAAAGGCAATGACAAAGTTCATGGTGGAGTCCACCACAAAAATGGTAAAAGAGTGGAGAAGCCTGGTAAAGTCGGGGAAACCGGAAATCGACGTGGAGAGAGAAATCACGGCAACGGCCGGGGACATAATCGCAAAGGCAAGCTTCGGAATTTGGAGTGGCGGCGGCGGTGAAGAGGCATTGAATAACCTGAGAGCTCTTCAATTGACTCTGTTCTCGAACAGCAGCTACGTGGGGGTCCCATTCAGCAGTATATTTCTGTACCCTGCTCGAACCGTAGCCGCCAAGAGACTCGGTGCCCAAATCGACCGCCACTTCTTTTCCATCATATCTCACAGGAAACTCTCTTCCGATTCCGATTCCGATTTGCTGAGTCGGATGATTCAGAGTCAAAGCAACCAGAAGCAGGGCAGCGCCGCCCAGCTGACCCCTAGAGAAGTGGTGGACGAGTGTAAGACTTTCTTCTTCGGTGGCCATGAGACGACGGCGCTTGCCATCAGTTGGACGCTGCTGCTGCTGGCCACGCGCCCCGATTGGCAAAACATTTTACGAGAGGAGATTAAAGAAGTTATCGGAGATAAAGATATTGATTTCTCCATGCTTTCTTCACTCAAAAAGGTTTCTCCATATTTTCTCTTTTTCCTATTTGCTCTGCTCATATGA

Protein sequence

MELICGLIMLIMWVIRVSIMVLFVKVVVSWWILPILAHKKLKRNGFEGPSPTFPLGNITEMKKIMKAAAAINGSSTTSLDASSNNVSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIADPQLVKKISEAVMGKSWGKPTVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPTNLKAMTKFMVESTTKMVKEWRSLVKSGKPEIDVEREITATAGDIIAKASFGIWSGGGGEEALNNLRALQLTLFSNSSYVGVPFSSIFLYPARTVAAKRLGAQIDRHFFSIISHRKLSSDSDSDLLSRMIQSQSNQKQGSAAQLTPREVVDECKTFFFGGHETTALAISWTLLLLATRPDWQNILREEIKEVIGDKDIDFSMLSSLKKVSPYFLFFLFALLI

Homology

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BLAST of Lsi05G012190 vs. Swiss-Prot
Match: C7351_ARATH (Cytokinin hydroxylase OS=Arabidopsis thaliana GN=CYP735A1 PE=1 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 8.9e-50
Identity = 130/398 (32.66%), Postives = 196/398 (49.25%), Query Frame = 1


Query: 11  IMWVIRVSIMVLFVKVVVS--------WWILPILAHKKLKRNGFEGPSPTFPLGNITEMK 70
           ++  I  S++V+FV  ++         +W+ P    K +++ G  GP P    GNI E+ 
Sbjct: 1   MLLTILKSLLVIFVTTILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEIS 60

Query: 71  KIMKAAAAINGSSTTSLDASSNNVSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIA 130
            ++        S + S D  S  + HDI   + PH+  W   YGK+F+ W GT+P L + 
Sbjct: 61  AMV--------SQSASKDCDS--IHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLT 120

Query: 131 DPQLVKKI---SEAVMGKSWGKPTVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPT 190
           + +L+K++      V G+SW +    +N      G GL+M  G DW   RH+  PAF   
Sbjct: 121 ETELIKELLMKHNGVSGRSWLQQQGTKN----FIGRGLLMANGQDWHHQRHLAAPAFTGE 180

Query: 191 NLKAMTKFMVESTTKMVKEWRSLVKSGKPEIDVEREITATAGDIIAKASFGIWSGGGGEE 250
            LK   + MVE T+K+V+  R  V  G  E+++  E+     DII++  FG  S   G+E
Sbjct: 181 RLKGYARHMVECTSKLVERLRKEVGEGANEVEIGEEMHKLTADIISRTKFGS-SFEKGKE 240

Query: 251 ALNNLRALQLTLFSNSSYVGVPFSSIFLYPARTVAAKRLGAQIDRHFFSIISHRK----- 310
             N+L  LQ      + ++  P  S FL        K L  +++R    II  R+     
Sbjct: 241 LFNHLTVLQRRCAQATRHLCFP-GSRFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEM 300

Query: 311 -LSSDSDSDLLSRMIQSQSNQKQGSAAQLTPREVVDECKTFFFGGHETTALAISWTLLLL 370
             SS    DLL  ++      K  +      + ++DECKTFFF GHETTAL ++WT +LL
Sbjct: 301 GRSSTHGDDLLGLLLNEMDIDKNNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLL 360

Query: 371 ATRPDWQNILREEIKEVIGDKDI----DFSMLSSLKKV 388
           A  P WQ  +REE++EV G   +      S L+SL KV
Sbjct: 361 ADNPTWQEKVREEVREVFGRNGLPSVDQLSKLTSLSKV 382

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BLAST of Lsi05G012190 vs. Swiss-Prot
Match: C7091_ARATH (Cytochrome P450 709B1 OS=Arabidopsis thaliana GN=CYP709B1 PE=2 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 6.4e-48
Identity = 134/390 (34.36%), Postives = 211/390 (54.10%), Query Frame = 1


Query: 9   MLIMWVIRVSIMVLFVKVVVSWWIL---PILAHKKLKRNGFEGPSPTFPLGNITEMKKIM 68
           ++I   + V I+++ +++  ++ IL   P +  ++LK  G  GP+     GN++E+KK+ 
Sbjct: 3   LVIFLALIVLILIIGLRIFKAFMILVWHPFVLTRRLKNQGISGPNYRIFYGNLSEIKKMK 62

Query: 69  KAAAAINGSSTTSLDASSNNVSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIADPQ 128
           +       S  + LD SSN    DI   I PH+ +W + YG+ F+YW GTEP + I+DP+
Sbjct: 63  RE------SHLSILDPSSN----DIFPRILPHYQKWMSQYGETFLYWNGTEPRICISDPE 122

Query: 129 LVKKISEAVMG---KSWGKPTVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPTNLK 188
           L K +    +G   KS  +P   +     +   GLV  EG DW+RHR IL PAF+   LK
Sbjct: 123 LAKTMLSNKLGFFVKSKARPEAVKL----VGSKGLVFIEGADWVRHRRILNPAFSIDRLK 182

Query: 189 AMTKFMVESTTKMVKEWR---SLVKSGKPEI--DVEREITATAGDIIAKASFGIWSGGGG 248
            MT  MV+ T KM++EWR   +  ++  P+I  ++  E      DIIA ++FG  S   G
Sbjct: 183 IMTTVMVDCTLKMLEEWRKESTKEETEHPKIKKEMNEEFQRLTADIIATSAFGS-SYVEG 242

Query: 249 EEALNNLRALQLTLFSNSSYVGVPFSSIFLYPARTVAAKRLGAQIDRHFFSIISHRKLS- 308
            E   +   L+    ++ + V +P +     P+  +   +L  ++D     IIS R  S 
Sbjct: 243 IEVFRSQMELKRCYTTSLNQVSIPGTQYLPTPSN-IRVWKLERKMDNSIKRIISSRLQSK 302

Query: 309 SDSDSDLLSRMIQSQSNQKQGSAAQLTPREVVDECKTFFFGGHETTALAISWTLLLLATR 368
           SD   DLL  ++++ + +  G   +++  E++ EC+TFFFGGHETT+  ++WT +LL+  
Sbjct: 303 SDYGDDLLGILLKAYNTE--GKERKMSIEEIIHECRTFFFGGHETTSNLLAWTTMLLSLH 362

Query: 369 PDWQNILREEI-KEVIGDKDIDFSMLSSLK 386
            DWQ  LREEI KE   +K  D    S LK
Sbjct: 363 QDWQEKLREEIFKECGKEKTPDSETFSKLK 374

[Close]

BLAST of Lsi05G012190 vs. Swiss-Prot
Match: C7352_ARATH (Cytokinin hydroxylase OS=Arabidopsis thaliana GN=CYP735A2 PE=1 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 2.7e-46
Identity = 128/398 (32.16%), Postives = 199/398 (50.00%), Query Frame = 1


Query: 7   LIMLIMWVIRVSIMVLFVKV----VVSWWILPILAHKKLKRNGFEGPSPTFPLGNITEMK 66
           ++ L++  + V +M L ++V    +  +++ P    K ++R G  GP P    GNI ++ 
Sbjct: 2   MVTLVLKYVLVIVMTLILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNIIDIS 61

Query: 67  KIMKAAAAINGSSTTSLDASSNNVSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIA 126
           K++        S + S D SS  + H+I   + PH+  W   YGK+F+ W GTEP L + 
Sbjct: 62  KML--------SHSASNDCSS--IHHNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLT 121

Query: 127 DPQLVKKI---SEAVMGKSWGKPTVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPT 186
           + +++K++      V GKSW    + +   +   G GL+M  G+ W   RH+  PAF   
Sbjct: 122 ETEMIKELLTKHNPVTGKSW----LQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRD 181

Query: 187 NLKAMTKFMVESTTKMVKEWRSLVKSGKPEIDVEREITATAGDIIAKASFGIWSGGGGEE 246
            LK   K MVE T  M +  R   K    E+++  E+     DII++  FG  S   G+E
Sbjct: 182 RLKGYAKHMVECTKMMAERLR---KEVGEEVEIGEEMRRLTADIISRTEFGS-SCDKGKE 241

Query: 247 ALNNLRALQLTLFSNSSYVGVPFSSIFLYPARTVAAKRLGAQIDRHFFSIISHRK----- 306
             + L  LQ      + ++  P  S FL        K L  +++R    II  RK     
Sbjct: 242 LFSLLTVLQRLCAQATRHLCFP-GSRFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEI 301

Query: 307 -LSSDSDSDLLSRMIQSQSNQKQGSAAQLTPREVVDECKTFFFGGHETTALAISWTLLLL 366
             SS    DLL  ++    NQ   +   L  + ++DECKTFFF GHETT+L ++WTL+LL
Sbjct: 302 GRSSSYGDDLLGLLL----NQMDSNKNNLNVQMIMDECKTFFFTGHETTSLLLTWTLMLL 361

Query: 367 ATRPDWQNILREEIKEVIGDKDI----DFSMLSSLKKV 388
           A  P WQ+ +R+E+++V G   +      S L+SL KV
Sbjct: 362 AHNPTWQDNVRDEVRQVCGQDGVPSVEQLSSLTSLNKV 376

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BLAST of Lsi05G012190 vs. Swiss-Prot
Match: C14B1_ORYSJ (Cytochrome P450 714B1 OS=Oryza sativa subsp. japonica GN=CYP714B1 PE=1 SV=2)

HSP 1 Score: 184.5 bits (467), Expect = 2.3e-45
Identity = 108/368 (29.35%), Postives = 186/368 (50.54%), Query Frame = 1


Query: 31  WILPILAHKKLKRNGFEGPSPTFPLGNITEMKKIMKAAAAINGSSTTSLDASSNNVSHDI 90
           W+ P      L+R G  GP+P+FP GN+ +M+    AAA   G   T       ++ HD 
Sbjct: 27  WLAPERLRAHLRRQGIGGPTPSFPYGNLADMRSHAAAAA---GGKATGEGRQEGDIVHDY 86

Query: 91  HSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIADPQLVKKISEAVMGKSWGKPTVFRNDRE 150
              +FP +  W+  YG  F Y +G   FL+++ P +V+++S  V     GK +  +   +
Sbjct: 87  RQAVFPFYENWRKQYGPVFTYSVGNMVFLHVSRPDIVRELSLCV-SLDLGKSSYMKATHQ 146

Query: 151 PMFGDGLVMTEGDDWIRHRHILTPAFNPTNLKAMTKFMVESTTKMVKEWRSLV-KSGKPE 210
           P+FG+G++ + G+ W   R ++ P F P  +K M   MV+S   +V  W   + +SG   
Sbjct: 147 PLFGEGILKSNGNAWAHQRKLIAPEFFPDKVKGMVDLMVDSAQVLVSSWEDRIDRSGGNA 206

Query: 211 ID--VEREITATAGDIIAKASFGIWSGGGGEEALNNLRALQLTLFSNSSYVGVPFSSI-F 270
           +D  ++ +I A + D+I++  FG  S   G++  + +R LQ T+ +    +    + + F
Sbjct: 207 LDLMIDDDIRAYSADVISRTCFGS-SYVKGKQIFDMIRELQKTVSTKKQNLLAEMTGLSF 266

Query: 271 LYP-ARTVAAKRLGAQIDRHFFSIISHRKLSSDSDSDLLSRMIQSQSNQKQGSAAQLTPR 330
           L+P A   AA RL  ++      ++       +   +LLS M++S      G        
Sbjct: 267 LFPKASGRAAWRLNGRVRALILDLVGEN--GEEDGGNLLSAMLRSARGGGGGGGEVAAAA 326

Query: 331 E--VVDECKTFFFGGHETTALAISWTLLLLATRPDWQNILREEIKEVI---GDKDIDFSM 389
           E  VVD CK  +F G+E+TA+  +W L+LLA  P+WQ+ +R+E++      G +  DF  
Sbjct: 327 EDFVVDNCKNIYFAGYESTAVTAAWCLMLLALHPEWQDRVRDEVQAACCGGGGRSPDFPA 386

[Close]

BLAST of Lsi05G012190 vs. Swiss-Prot
Match: C14B3_MAIZE (Cytochrome P450 714B3 OS=Zea mays GN=CYP714B3 PE=2 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 8.7e-45
Identity = 107/361 (29.64%), Postives = 181/361 (50.14%), Query Frame = 1


Query: 31  WILPILAHKKLKRNGFEGPSPTFPLGNITEMKKIMKAAAAINGSSTTSLDASSNNVSHDI 90
           W++P     K +     GP P+FP GN+ +M++   AA A   S+  S  +    + HD 
Sbjct: 32  WLVPQKVLAKFEDQKIGGPRPSFPYGNLADMREAAAAAKAARASARRS-GSGGGGIVHDY 91

Query: 91  HSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIADPQLVKKISEAVMGKSWGKPTVFRNDRE 150
              + P++ +W+  YG  F Y +G   FL+++ P +V+ I+  V     GK +  +   E
Sbjct: 92  RPAVLPYYEKWRKEYGPIFTYSMGNVVFLHVSRPDVVRDINLCV-SLDLGKSSYLKATHE 151

Query: 151 PMFGDGLVMTEGDDWIRHRHILTPAFNPTNLKAMTKFMVESTTKMVKEWRSLV--KSGKP 210
           P+FG G++ + G+ W+  R I+ P F    +K M   MV+S   ++  W   V    G  
Sbjct: 152 PLFGGGILKSNGEAWLHQRKIIAPEFFLDKVKGMVDLMVDSAQPLLMSWEERVDRNGGIT 211

Query: 211 EIDVEREITATAGDIIAKASFGIWSGGGGEEALNNLRALQLTLFSNSSYVGVPFSSIFLY 270
           +I ++ +I A + D+I++  FG  S   G+E    +R LQ  +  +   V    + +  +
Sbjct: 212 DIKIDDDIRAYSADVISRTCFGS-SYIKGKEIFMKIRELQQAV--SKPNVLAEMTGLRFF 271

Query: 271 PA-RTVAAKRLGAQIDRHFFSIISHRKLSSDSDSDLLSRMIQSQSNQKQGSAAQLTPREV 330
           P+ R   A  L  Q+ +    I+      S  D +LLS ++ S S  + G A       +
Sbjct: 272 PSMRNKQAWELHKQVRKLILEIVKE----SGEDRNLLSAILHSASTSRVGIAE--AENFI 331

Query: 331 VDECKTFFFGGHETTALAISWTLLLLATRPDWQNILREEIKEVIGDKDIDFSMLSSLKKV 389
           VD CK+ +F GHE+TA+  +W L+LL   P+WQN +REE+ EV   + +D   L  +K +
Sbjct: 332 VDNCKSIYFAGHESTAVTAAWCLMLLGLHPEWQNRVREEVHEVCRGQPVDSRSLQKMKNL 381

[Close]

BLAST of Lsi05G012190 vs. TrEMBL
Match: A0A0A0L9T4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G182180 PE=4 SV=1)

HSP 1 Score: 598.6 bits (1542), Expect = 5.6e-168
Identity = 315/384 (82.03%), Postives = 333/384 (86.72%), Query Frame = 1


Query: 20  MVLFVKVVVSWWILPILAHKKLKRNGFEGPSPTFPLGNITEMKKIMKAAAA----INGSS 79
           MVLF KVV+S WILP LAHKKLKRNGFEGPSP+FPLGNITEMKKIMKAAAA    INGSS
Sbjct: 1   MVLFGKVVISCWILPTLAHKKLKRNGFEGPSPSFPLGNITEMKKIMKAAAAGGAAINGSS 60

Query: 80  TTSLDASSNNVSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIADPQLVKKISEAVM 139
                  SNN+SHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIADPQLVKKISEAV+
Sbjct: 61  -------SNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIADPQLVKKISEAVL 120

Query: 140 GKSWGKPTVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPTNLKAMTKFMVESTTKM 199
           GKSWGKP VFRNDREPMFGDGLVMTEGD+WIRHRHILTPAFNP NLKAMTKFMVEST KM
Sbjct: 121 GKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPANLKAMTKFMVESTKKM 180

Query: 200 VKEWRSLVKSGKPEIDVEREITATAGDIIAKASFGIWSGGGGEEALNNLRALQLTLFSNS 259
           ++EWR  VKSG+ EI+VEREITATAG+IIAKASFGI   GGG EALNNLRALQLTLF NS
Sbjct: 181 LEEWRRQVKSGQSEIEVEREITATAGEIIAKASFGISKCGGGGEALNNLRALQLTLFKNS 240

Query: 260 SYVGVPFSSIFLYPARTVAAKRLGAQIDRHFFSIISHRKLSSDSD--SDLLSRMIQSQSN 319
           SYVGVPFSS+ LYPART+AAKRLGAQIDR FFSIIS RK+S      SDLLSR+I S+  
Sbjct: 241 SYVGVPFSSLVLYPARTLAAKRLGAQIDRLFFSIISDRKISGSGSAYSDLLSRLIGSEYK 300

Query: 320 QKQGSAAQLTPREVVDECKTFFFGGHETTALAISWTLLLLATRPDWQNILREEIKEVIGD 379
                 A L+PREVVDECKTFFFGGHETTALAISWTLLLLAT PDWQ ILR+EIKEVIGD
Sbjct: 301 ----GGAGLSPREVVDECKTFFFGGHETTALAISWTLLLLATSPDWQTILRDEIKEVIGD 360

Query: 380 KDIDFSMLSSLKKVS-PYFLFFLF 397
           KDIDFSMLSSLKKV   +FL F F
Sbjct: 361 KDIDFSMLSSLKKVPFSFFLSFFF 373

[Close]

BLAST of Lsi05G012190 vs. TrEMBL
Match: I6XNC3_LINUS (Putative cytochrome P450 protein OS=Linum usitatissimum PE=3 SV=1)

HSP 1 Score: 438.7 bits (1127), Expect = 7.5e-120
Identity = 218/368 (59.24%), Postives = 281/368 (76.36%), Query Frame = 1


Query: 26  VVVSWWILPILAHKKLKRNGFEGPSPTFPLGNITEMKKIMKAAAAINGSSTTSLDASSNN 85
           +V ++W+ P+ A+ KL+RNGF GP+P+FPLGNI EM+ I K    ++ SS+         
Sbjct: 22  LVYAFWVSPLAAYYKLRRNGFGGPTPSFPLGNIKEMRTIAK--LTVSSSSSGGGCGGGGG 81

Query: 86  VSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIADPQLVKKISEAVMGKSWGKPTVF 145
           +SHDIHS +FP+F+QWQNS+GK FVYWLGTEPF+YIADP+ +K++S  VMGKSWGKP VF
Sbjct: 82  ISHDIHSNVFPYFSQWQNSHGKVFVYWLGTEPFMYIADPEFLKEMSAGVMGKSWGKPRVF 141

Query: 146 RNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPTNLKAMTKFMVESTTKMVKEWRSLVKS 205
           + DREPMFG+GLVM EGDDW+RHRH++TPAF P+NLKAM+  MVESTT+M+ EW +L+ S
Sbjct: 142 KQDREPMFGNGLVMVEGDDWVRHRHVITPAFTPSNLKAMSSLMVESTTRMLDEWATLISS 201

Query: 206 GKPEIDVEREITATAGDIIAKASFGIWSGGGGEEALNNLRALQLTLFSNSSYVGVPFSSI 265
           G PEI+VE  ITATAG+IIAK SFG+     G   +  LRA+QLTLF+++ YVGVP+S I
Sbjct: 202 GVPEIEVESHITATAGEIIAKTSFGVGYNESGRMVVEKLRAMQLTLFNSNRYVGVPYSKI 261

Query: 266 FLYPARTVAAKRLGAQIDRHFFSIISHRKLS----SDSDSDLLSRMIQSQSNQKQGSAAQ 325
            + P +T+ AK+LG +ID    SIIS RK S    ++++ DLL  ++  Q N   G   +
Sbjct: 262 -MCPRKTLDAKKLGKEIDSLLLSIISTRKNSLNTTTNNNKDLLGLLL--QENHADGRLGK 321

Query: 326 -LTPREVVDECKTFFFGGHETTALAISWTLLLLATRPDWQNILREEIKEVIGDKD-IDFS 385
            LT RE+VDECKTFFFGGHETTALAISWTL+LLA  P+WQ+ LR+EIKEV GDK  +DF+
Sbjct: 322 TLTTRELVDECKTFFFGGHETTALAISWTLMLLAKHPEWQDELRKEIKEVSGDKTRVDFA 381

Query: 386 MLSSLKKV 388
           MLSSLKK+
Sbjct: 382 MLSSLKKM 384

[Close]

BLAST of Lsi05G012190 vs. TrEMBL
Match: A0A0D2UAW4_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_008G276600 PE=3 SV=1)

HSP 1 Score: 437.6 bits (1124), Expect = 1.7e-119
Identity = 220/369 (59.62%), Postives = 277/369 (75.07%), Query Frame = 1


Query: 23  FVKVVVSWWILPILAHKKLKRNGFEGPSPTFPLGNITEMKKIMKAAAAINGSSTTSLDAS 82
           F  ++ S W+LPI A+KK+K+NGF GP P+FP+GNITEMK  M     IN SS      S
Sbjct: 23  FSTLIFSCWVLPIRAYKKIKKNGFTGPIPSFPMGNITEMKNSMN----INVSS------S 82

Query: 83  SNNVSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIADPQLVKKISEAVMGKSWGKP 142
            N VSHDIHST+FP+FA+WQ SYGK F+YWLGTEPFLY+A+P+ +K +S  V+GKSWGKP
Sbjct: 83  LNVVSHDIHSTVFPYFARWQKSYGKLFIYWLGTEPFLYVAEPEFLKNMSAGVLGKSWGKP 142

Query: 143 TVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPTNLKAMTKFMVESTTKMVKEWRSL 202
            VF++DREPMFG GLVM EGD+W+RHRH++TPAF+P NLKAM   MVE TTKM+ +W +L
Sbjct: 143 KVFKHDREPMFGSGLVMVEGDEWVRHRHVITPAFSPCNLKAMASLMVEPTTKMLDQWATL 202

Query: 203 VKSGKPEIDVEREITATAGDIIAKASFGIWSGGGGEEALNNLRALQLTLFSNSSYVGVPF 262
           + SG PEIDVEREIT TAG+IIA+ SFG+ S   G +    LRA+Q+TLF+++ YVGVPF
Sbjct: 203 ISSGYPEIDVEREITTTAGEIIARTSFGL-SYQNGNKVFEKLRAMQITLFNSNRYVGVPF 262

Query: 263 SSIFLYPARTVAAKRLGAQIDRHFFSIISHRKLSSD----SDSDLLSRMIQSQSNQKQGS 322
           S  ++ P + + AKRLG +ID+   SII  RK S D       DLL  +++      + S
Sbjct: 263 SK-WMCPKKNLEAKRLGKEIDQLLLSIIDARKKSLDIGTSPQKDLLGLLMEGGLVDGR-S 322

Query: 323 AAQLTPREVVDECKTFFFGGHETTALAISWTLLLLATRPDWQNILREEIKEVIGDKDIDF 382
              LT RE+VDECKTFFFGGHETTALA++WTLLLLAT PDWQN LR+EIK+VIGD +IDF
Sbjct: 323 GKSLTARELVDECKTFFFGGHETTALALTWTLLLLATYPDWQNQLRDEIKQVIGDGEIDF 378

Query: 383 SMLSSLKKV 388
           + L+ LKK+
Sbjct: 383 TKLADLKKM 378

[Close]

BLAST of Lsi05G012190 vs. TrEMBL
Match: M5X7L0_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa024029mg PE=3 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 2.2e-119
Identity = 216/374 (57.75%), Postives = 287/374 (76.74%), Query Frame = 1


Query: 17  VSIMVLFVKVVVSWWILPILAHKKLKRNGFEGPSPTFPLGNITEMKKIMKAAAAINGSST 76
           ++++ +   ++ S+W+ P+LA++KLK+ G  GPSP+FPLGN+TEMKK +     +  SS+
Sbjct: 1   MALLYMCCSLIYSFWVSPVLAYRKLKKYGLNGPSPSFPLGNLTEMKKKIN----VKSSSS 60

Query: 77  TSLDASSNNVSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIADPQLVKKISEAVMG 136
           +    +SN ++HDIHST+FP+FA+WQ+S+GK FVYWLGTEPFLYIADP+L+KK+S  V  
Sbjct: 61  SFGQPNSNIITHDIHSTLFPYFARWQSSHGKMFVYWLGTEPFLYIADPELLKKLSTEVTA 120

Query: 137 KSWGKPTVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPTNLKAMTKFMVESTTKMV 196
           K+WGKP VFR DR  MFG+GLVM+EGDDW+RHRH++TPAFNPTNLKAM   MVE+TT+M+
Sbjct: 121 KNWGKPAVFRRDRAAMFGNGLVMSEGDDWVRHRHVITPAFNPTNLKAMASLMVETTTEML 180

Query: 197 KEWRSLVKSGKPEIDVEREITATAGDIIAKASFGIWSGGGGEEALNNLRALQLTLFSNSS 256
             WR L+ SG  EID+EREITATAG+IIAK SFGI S   G      LRALQ+TLF  S 
Sbjct: 181 DNWRVLIDSGSQEIDIEREITATAGEIIAKTSFGI-SYQSGRLVYEKLRALQMTLFKTSR 240

Query: 257 YVGVPFSSIFLYPARTVAAKRLGAQIDRHFFSIISHRKLS---SDSDSDLLSRMIQSQSN 316
           +VGVPF  + ++P +T+ A+RLG +I++ F SIIS RK S   S    DLL  ++  + +
Sbjct: 241 FVGVPFGKM-MHPKKTLEARRLGQEINQLFLSIISARKKSIRESTQQHDLLGILL--KQS 300

Query: 317 QKQGSAAQLTPREVVDECKTFFFGGHETTALAISWTLLLLATRPDWQNILREEIKEVIGD 376
           ++ G    LT +++VDECKTFFFGGHETTALAI+WT+LLLAT  DWQ+ LREEI+EV+GD
Sbjct: 301 EQGGFTKALTTQDLVDECKTFFFGGHETTALAITWTMLLLATHQDWQDQLREEIREVVGD 360

Query: 377 KDIDFSMLSSLKKV 388
           K+ID +ML+ LKK+
Sbjct: 361 KEIDVNMLAGLKKM 366

[Close]

BLAST of Lsi05G012190 vs. TrEMBL
Match: I6YHZ8_LINUS (Cytochrome P450 OS=Linum usitatissimum GN=cyp450 PE=3 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 6.3e-119
Identity = 215/367 (58.58%), Postives = 277/367 (75.48%), Query Frame = 1


Query: 26  VVVSWWILPILAHKKLKRNGFEGPSPTFPLGNITEMKKIMKAAAAINGSSTTSLDASSNN 85
           +V ++W+ P+ A+ KL+RNGF GP+P+FPLGNI EM+ I K  A  +             
Sbjct: 15  LVYAFWVSPLAAYYKLRRNGFGGPAPSFPLGNIKEMRTIAKLTAVSSSGGA--------G 74

Query: 86  VSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIADPQLVKKISEAVMGKSWGKPTVF 145
           +SHDIHS +FP+FAQWQ S+GK FVYWLGTEPF+YIA+P+ +K++S  VMGKSWGKP VF
Sbjct: 75  ISHDIHSNVFPYFAQWQKSHGKVFVYWLGTEPFMYIANPEFLKEMSAGVMGKSWGKPRVF 134

Query: 146 RNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPTNLKAMTKFMVESTTKMVKEWRSLVKS 205
           + DREPMFG+GLVM EGDDW+RHRH++TPAF P+NLKAM+  MVESTT+M+ EW +L+ S
Sbjct: 135 KQDREPMFGNGLVMVEGDDWVRHRHVITPAFTPSNLKAMSSLMVESTTRMLGEWDALISS 194

Query: 206 GKPEIDVEREITATAGDIIAKASFGIWSGGGGEEALNNLRALQLTLFSNSSYVGVPFSSI 265
           G PEI+VE  ITATAG+IIAK SFG+     G   +  LRA+QLTLF+++ YVGVP+S I
Sbjct: 195 GVPEIEVESHITATAGEIIAKTSFGVGYNESGRMVVEKLRAMQLTLFNSNRYVGVPYSKI 254

Query: 266 FLYPARTVAAKRLGAQIDRHFFSIISHRK----LSSDSDSDLLSRMIQSQSNQKQGSAAQ 325
            + P +T+ AK+LG +ID    SIIS RK    ++++++ DLL  ++  Q N   G   +
Sbjct: 255 -MCPRKTLDAKKLGKEIDSLLLSIISTRKNNLNITTNNNKDLLGLLL--QENHADGRLGK 314

Query: 326 -LTPREVVDECKTFFFGGHETTALAISWTLLLLATRPDWQNILREEIKEVIGDKDIDFSM 385
            LT RE+VDECKTFFFGGHETTALAISWTL+LLA  P+WQ+ LR+EIKEV  DK +DFSM
Sbjct: 315 TLTTRELVDECKTFFFGGHETTALAISWTLMLLAKHPEWQDELRKEIKEVSKDKMVDFSM 370

Query: 386 LSSLKKV 388
           LSSLKK+
Sbjct: 375 LSSLKKM 370

[Close]

BLAST of Lsi05G012190 vs. TAIR10
Match: AT5G52400.1 (AT5G52400.1 cytochrome P450, family 715, subfamily A, polypeptide 1)

HSP 1 Score: 404.1 bits (1037), Expect = 1.0e-112
Identity = 199/379 (52.51%), Postives = 263/379 (69.39%), Query Frame = 1


Query: 14  VIRVSIMVLFVKVVVSWWILPILAHKKLKRNGFEGPSPTFPLGNITEMKKIMKAAAAING 73
           V  V I ++F+K+ +  WI P+ A KKL+ NGF GP+P+FP GN+ +MKK+  A+  ++ 
Sbjct: 16  VFVVVICLMFLKLFLRCWIWPVRAQKKLRGNGFVGPAPSFPFGNLNDMKKLKMASVVVDN 75

Query: 74  SSTTSLDASSNNVSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIADPQLVKKISEA 133
           S ++++      ++HDIHS   PHFA+WQ  YGK FVYWLG EPF+Y+ADP+ +  +S+ 
Sbjct: 76  SKSSTI------INHDIHSIALPHFARWQQEYGKVFVYWLGIEPFVYVADPEFLSVMSKG 135

Query: 134 VMGKSWGKPTVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPTNLKAMTKFMVESTT 193
           V+GKSWGKP VF+ DREPMFG GLVM EGDDW RHRHI+TPAF P NLK MT  MVES +
Sbjct: 136 VLGKSWGKPNVFKKDREPMFGTGLVMVEGDDWTRHRHIITPAFAPLNLKVMTNMMVESVS 195

Query: 194 KMVKEWRSLVKSGKPEIDVEREITATAGDIIAKASFGIWSGGGGEEALNNLRALQLTLFS 253
            M+  W   + SG PE D+E EI  TAG+IIAK SFG+ +G  G + L NLRA+Q  LF+
Sbjct: 196 NMLDRWGIQINSGNPEFDMESEIIGTAGEIIAKTSFGV-TGENGTQVLKNLRAVQFALFN 255

Query: 254 NSSYVGVPFSSIFLYPARTVAAKRLGAQIDRHFFSIISHRKLS----SDSDSDLLSRMIQ 313
           ++ YVGVPFS+I  Y  +TV AK LG +ID    S I+ RK+S     D   DLL  ++ 
Sbjct: 256 SNRYVGVPFSNILSY-KQTVKAKGLGHEIDGLLLSFINKRKISLAEGDDQGHDLLGMLL- 315

Query: 314 SQSNQKQGSAAQLTPREVVDECKTFFFGGHETTALAISWTLLLLATRPDWQNILREEIKE 373
                K       T +E+VDECKTFFF GHETTALA++WT +LLA  P+WQ+ +REEI+E
Sbjct: 316 -----KADQKGNFTAKELVDECKTFFFAGHETTALALTWTFMLLAIHPEWQDTIREEIRE 375

Query: 374 VIGDKDIDFSMLSSLKKVS 389
           VIGD  I+++ L+ LKK+S
Sbjct: 376 VIGDSKIEYNKLAGLKKMS 380

[Close]

BLAST of Lsi05G012190 vs. TAIR10
Match: AT5G38450.1 (AT5G38450.1 cytochrome P450, family 735, subfamily A, polypeptide 1)

HSP 1 Score: 199.1 bits (505), Expect = 5.0e-51
Identity = 130/398 (32.66%), Postives = 196/398 (49.25%), Query Frame = 1


Query: 11  IMWVIRVSIMVLFVKVVVS--------WWILPILAHKKLKRNGFEGPSPTFPLGNITEMK 70
           ++  I  S++V+FV  ++         +W+ P    K +++ G  GP P    GNI E+ 
Sbjct: 1   MLLTILKSLLVIFVTTILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEIS 60

Query: 71  KIMKAAAAINGSSTTSLDASSNNVSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIA 130
            ++        S + S D  S  + HDI   + PH+  W   YGK+F+ W GT+P L + 
Sbjct: 61  AMV--------SQSASKDCDS--IHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLT 120

Query: 131 DPQLVKKI---SEAVMGKSWGKPTVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPT 190
           + +L+K++      V G+SW +    +N      G GL+M  G DW   RH+  PAF   
Sbjct: 121 ETELIKELLMKHNGVSGRSWLQQQGTKN----FIGRGLLMANGQDWHHQRHLAAPAFTGE 180

Query: 191 NLKAMTKFMVESTTKMVKEWRSLVKSGKPEIDVEREITATAGDIIAKASFGIWSGGGGEE 250
            LK   + MVE T+K+V+  R  V  G  E+++  E+     DII++  FG  S   G+E
Sbjct: 181 RLKGYARHMVECTSKLVERLRKEVGEGANEVEIGEEMHKLTADIISRTKFGS-SFEKGKE 240

Query: 251 ALNNLRALQLTLFSNSSYVGVPFSSIFLYPARTVAAKRLGAQIDRHFFSIISHRK----- 310
             N+L  LQ      + ++  P  S FL        K L  +++R    II  R+     
Sbjct: 241 LFNHLTVLQRRCAQATRHLCFP-GSRFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEM 300

Query: 311 -LSSDSDSDLLSRMIQSQSNQKQGSAAQLTPREVVDECKTFFFGGHETTALAISWTLLLL 370
             SS    DLL  ++      K  +      + ++DECKTFFF GHETTAL ++WT +LL
Sbjct: 301 GRSSTHGDDLLGLLLNEMDIDKNNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLL 360

Query: 371 ATRPDWQNILREEIKEVIGDKDI----DFSMLSSLKKV 388
           A  P WQ  +REE++EV G   +      S L+SL KV
Sbjct: 361 ADNPTWQEKVREEVREVFGRNGLPSVDQLSKLTSLSKV 382

[Close]

BLAST of Lsi05G012190 vs. TAIR10
Match: AT2G46960.2 (AT2G46960.2 cytochrome P450, family 709, subfamily B, polypeptide 1)

HSP 1 Score: 193.0 bits (489), Expect = 3.6e-49
Identity = 134/390 (34.36%), Postives = 211/390 (54.10%), Query Frame = 1


Query: 9   MLIMWVIRVSIMVLFVKVVVSWWIL---PILAHKKLKRNGFEGPSPTFPLGNITEMKKIM 68
           ++I   + V I+++ +++  ++ IL   P +  ++LK  G  GP+     GN++E+KK+ 
Sbjct: 3   LVIFLALIVLILIIGLRIFKAFMILVWHPFVLTRRLKNQGISGPNYRIFYGNLSEIKKMK 62

Query: 69  KAAAAINGSSTTSLDASSNNVSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIADPQ 128
           +       S  + LD SSN    DI   I PH+ +W + YG+ F+YW GTEP + I+DP+
Sbjct: 63  RE------SHLSILDPSSN----DIFPRILPHYQKWMSQYGETFLYWNGTEPRICISDPE 122

Query: 129 LVKKISEAVMG---KSWGKPTVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPTNLK 188
           L K +    +G   KS  +P   +     +   GLV  EG DW+RHR IL PAF+   LK
Sbjct: 123 LAKTMLSNKLGFFVKSKARPEAVKL----VGSKGLVFIEGADWVRHRRILNPAFSIDRLK 182

Query: 189 AMTKFMVESTTKMVKEWR---SLVKSGKPEI--DVEREITATAGDIIAKASFGIWSGGGG 248
            MT  MV+ T KM++EWR   +  ++  P+I  ++  E      DIIA ++FG  S   G
Sbjct: 183 IMTTVMVDCTLKMLEEWRKESTKEETEHPKIKKEMNEEFQRLTADIIATSAFGS-SYVEG 242

Query: 249 EEALNNLRALQLTLFSNSSYVGVPFSSIFLYPARTVAAKRLGAQIDRHFFSIISHRKLS- 308
            E   +   L+    ++ + V +P +     P+  +   +L  ++D     IIS R  S 
Sbjct: 243 IEVFRSQMELKRCYTTSLNQVSIPGTQYLPTPSN-IRVWKLERKMDNSIKRIISSRLQSK 302

Query: 309 SDSDSDLLSRMIQSQSNQKQGSAAQLTPREVVDECKTFFFGGHETTALAISWTLLLLATR 368
           SD   DLL  ++++ + +  G   +++  E++ EC+TFFFGGHETT+  ++WT +LL+  
Sbjct: 303 SDYGDDLLGILLKAYNTE--GKERKMSIEEIIHECRTFFFGGHETTSNLLAWTTMLLSLH 362

Query: 369 PDWQNILREEI-KEVIGDKDIDFSMLSSLK 386
            DWQ  LREEI KE   +K  D    S LK
Sbjct: 363 QDWQEKLREEIFKECGKEKTPDSETFSKLK 374

[Close]

BLAST of Lsi05G012190 vs. TAIR10
Match: AT1G67110.1 (AT1G67110.1 cytochrome P450, family 735, subfamily A, polypeptide 2)

HSP 1 Score: 187.6 bits (475), Expect = 1.5e-47
Identity = 128/398 (32.16%), Postives = 199/398 (50.00%), Query Frame = 1


Query: 7   LIMLIMWVIRVSIMVLFVKV----VVSWWILPILAHKKLKRNGFEGPSPTFPLGNITEMK 66
           ++ L++  + V +M L ++V    +  +++ P    K ++R G  GP P    GNI ++ 
Sbjct: 2   MVTLVLKYVLVIVMTLILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNIIDIS 61

Query: 67  KIMKAAAAINGSSTTSLDASSNNVSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIA 126
           K++        S + S D SS  + H+I   + PH+  W   YGK+F+ W GTEP L + 
Sbjct: 62  KML--------SHSASNDCSS--IHHNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLT 121

Query: 127 DPQLVKKI---SEAVMGKSWGKPTVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPT 186
           + +++K++      V GKSW    + +   +   G GL+M  G+ W   RH+  PAF   
Sbjct: 122 ETEMIKELLTKHNPVTGKSW----LQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRD 181

Query: 187 NLKAMTKFMVESTTKMVKEWRSLVKSGKPEIDVEREITATAGDIIAKASFGIWSGGGGEE 246
            LK   K MVE T  M +  R   K    E+++  E+     DII++  FG  S   G+E
Sbjct: 182 RLKGYAKHMVECTKMMAERLR---KEVGEEVEIGEEMRRLTADIISRTEFGS-SCDKGKE 241

Query: 247 ALNNLRALQLTLFSNSSYVGVPFSSIFLYPARTVAAKRLGAQIDRHFFSIISHRK----- 306
             + L  LQ      + ++  P  S FL        K L  +++R    II  RK     
Sbjct: 242 LFSLLTVLQRLCAQATRHLCFP-GSRFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEI 301

Query: 307 -LSSDSDSDLLSRMIQSQSNQKQGSAAQLTPREVVDECKTFFFGGHETTALAISWTLLLL 366
             SS    DLL  ++    NQ   +   L  + ++DECKTFFF GHETT+L ++WTL+LL
Sbjct: 302 GRSSSYGDDLLGLLL----NQMDSNKNNLNVQMIMDECKTFFFTGHETTSLLLTWTLMLL 361

Query: 367 ATRPDWQNILREEIKEVIGDKDI----DFSMLSSLKKV 388
           A  P WQ+ +R+E+++V G   +      S L+SL KV
Sbjct: 362 AHNPTWQDNVRDEVRQVCGQDGVPSVEQLSSLTSLNKV 376

[Close]

BLAST of Lsi05G012190 vs. TAIR10
Match: AT2G46950.1 (AT2G46950.1 cytochrome P450, family 709, subfamily B, polypeptide 2)

HSP 1 Score: 176.4 bits (446), Expect = 3.5e-44
Identity = 129/401 (32.17%), Postives = 205/401 (51.12%), Query Frame = 1


Query: 1   MELICGLIMLIMWVIRVSIMVLFVKVVVSWWILPILAHKKLKRNGFEGPSPTFPLGNITE 60
           MEL+  + +L + ++ + +  ++    +  W  P +  ++ K+ G  GP      GN+ E
Sbjct: 56  MELLSTINLLAIALVLLVVPKIYGACRILVW-RPWMLSRRFKKQGISGPKYRILYGNLRE 115

Query: 61  MKKIMKAAAAINGSSTTSLDASSNNVSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLY 120
           ++K+       N +    LD +SN    DI   + PH  QW++ YG+ F+YW GT+P L 
Sbjct: 116 IRKMK------NEAKLMVLDPNSN----DIVPRVLPHLQQWKSQYGETFLYWQGTDPRLC 175

Query: 121 IADPQLVKKISE---AVMGKSWGKPTVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFN 180
           I+D +L K+I         KS  KP + +     + G+GL+   G DW+RHR IL PAF+
Sbjct: 176 ISDHELAKQILSNKFVFFSKSKTKPEILK-----LSGNGLIFVNGLDWVRHRRILNPAFS 235

Query: 181 PTNLKAMTKFMVESTTKMVKEWRSLVKSGKPE--IDVEREITATAGDIIAKASFGIWSGG 240
              LK MT+ MV+ T +M  EW+      + E  + + RE      DIIA A+FG  S  
Sbjct: 236 MDKLKLMTQLMVDCTFRMFLEWKKQRNGVETEQFVLISREFKRLTADIIATAAFGS-SYA 295

Query: 241 GGEEALNNLRALQLTLFSNSSYVGVPFSSIFLYPART-VAAKRLGAQIDRHFFSIISHRK 300
            G E   +   L+L     ++   + F  I   P  + +   +L  +++     II  R 
Sbjct: 296 EGIEVFKS--QLELQKCCAAALTDLYFPGIQYLPTPSNLQIWKLDMKVNSSIKRIIDARL 355

Query: 301 LSSDSD--SDLLSRMIQSQSNQKQGSAAQLTPREVVDECKTFFFGGHETTALAISWTLLL 360
            S   D  +DLL  M+ + S+ +  S  +++  E+++ECKTFFF GHETTA  ++W+ +L
Sbjct: 356 TSESKDYGNDLLGIMLTAASSNE--SEKKMSIDEIIEECKTFFFAGHETTANLLTWSTML 415

Query: 361 LATRPDWQNILREEIKEVIG-DKDIDFSMLSSLKKVSPYFL 393
           L+   DWQ  LREE+    G DK  D    S LK ++  F+
Sbjct: 416 LSLHQDWQEKLREEVFNECGKDKIPDAETCSKLKLMNTVFM 435

[Close]

BLAST of Lsi05G012190 vs. NCBI nr
Match: gi|659077497|ref|XP_008439236.1| (PREDICTED: cytokinin hydroxylase [Cucumis melo])

HSP 1 Score: 626.7 bits (1615), Expect = 2.8e-176
Identity = 325/389 (83.55%), Postives = 349/389 (89.72%), Query Frame = 1


Query: 1   MELICGLIMLIMWVIRVSIMVLFVKVVVSWWILPILAHKKLKRNGFEGPSPTFPLGNITE 60
           MELIC LIMLI W+IRVSIMVLF KVV+S WILPILAH+KLKRNGFEGPSP+FPLGNITE
Sbjct: 1   MELICVLIMLIKWMIRVSIMVLFGKVVISCWILPILAHRKLKRNGFEGPSPSFPLGNITE 60

Query: 61  MKKIMKAAAAINGSSTTSLDASSNNVSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLY 120
           MKKIMKAAAA    +  S   SSNN+SHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLY
Sbjct: 61  MKKIMKAAAAATAVTNGS---SSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLY 120

Query: 121 IADPQLVKKISEAVMGKSWGKPTVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPTN 180
           IADPQLVKKISEAV+GKSWGKP VFRNDREPMFGDGLVMTEGD+WIRHRHILTPAFNPTN
Sbjct: 121 IADPQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTN 180

Query: 181 LKAMTKFMVESTTKMVKEWRSLVKSGKPEIDVEREITATAGDIIAKASFGIWSGGGGEEA 240
           LKAMTKFMVESTTKM++EWRSLVKSG+ EI+VER+ITATAG+IIAKASFGI + GGG EA
Sbjct: 181 LKAMTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIAKASFGISNCGGGGEA 240

Query: 241 LNNLRALQLTLFSNSSYVGVPFSSIFLYPARTVAAKRLGAQIDRHFFSIISHRKLSSDSD 300
           LNNLRALQLTLF NSSYVGVPFSS+FLYPART+AAKRLGAQIDR F SIIS+RK+S    
Sbjct: 241 LNNLRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMSGSGS 300

Query: 301 --SDLLSRMIQSQSNQKQGSAAQLTPREVVDECKTFFFGGHETTALAISWTLLLLATRPD 360
             SDLLSR+I S+          L+PREVVDECKTFFFGGHETTALAISWTLLLLAT PD
Sbjct: 301 GYSDLLSRLIGSEYT----GGEALSPREVVDECKTFFFGGHETTALAISWTLLLLATSPD 360

Query: 361 WQNILREEIKEVIGDKDIDFSMLSSLKKV 388
           WQ ILREEIKEV+GDKDIDFSMLSSLKK+
Sbjct: 361 WQRILREEIKEVVGDKDIDFSMLSSLKKM 382

[Close]

BLAST of Lsi05G012190 vs. NCBI nr
Match: gi|778679591|ref|XP_004151435.2| (PREDICTED: cytokinin hydroxylase [Cucumis sativus])

HSP 1 Score: 618.6 bits (1594), Expect = 7.6e-174
Identity = 325/393 (82.70%), Postives = 345/393 (87.79%), Query Frame = 1


Query: 1   MELICGLIMLIMWVIRVSIMVLFVKVVVSWWILPILAHKKLKRNGFEGPSPTFPLGNITE 60
           MELIC L+MLI W+IRVSIMVLF KVV+S WILP LAHKKLKRNGFEGPSP+FPLGNITE
Sbjct: 1   MELICVLVMLIKWMIRVSIMVLFGKVVISCWILPTLAHKKLKRNGFEGPSPSFPLGNITE 60

Query: 61  MKKIMKAAAA----INGSSTTSLDASSNNVSHDIHSTIFPHFAQWQNSYGKKFVYWLGTE 120
           MKKIMKAAAA    INGSS       SNN+SHDIHSTIFPHFAQWQNSYGKKFVYWLGTE
Sbjct: 61  MKKIMKAAAAGGAAINGSS-------SNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTE 120

Query: 121 PFLYIADPQLVKKISEAVMGKSWGKPTVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAF 180
           PFLYIADPQLVKKISEAV+GKSWGKP VFRNDREPMFGDGLVMTEGD+WIRHRHILTPAF
Sbjct: 121 PFLYIADPQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAF 180

Query: 181 NPTNLKAMTKFMVESTTKMVKEWRSLVKSGKPEIDVEREITATAGDIIAKASFGIWSGGG 240
           NP NLKAMTKFMVEST KM++EWR  VKSG+ EI+VEREITATAG+IIAKASFGI   GG
Sbjct: 181 NPANLKAMTKFMVESTKKMLEEWRRQVKSGQSEIEVEREITATAGEIIAKASFGISKCGG 240

Query: 241 GEEALNNLRALQLTLFSNSSYVGVPFSSIFLYPARTVAAKRLGAQIDRHFFSIISHRKLS 300
           G EALNNLRALQLTLF NSSYVGVPFSS+ LYPART+AAKRLGAQIDR FFSIIS RK+S
Sbjct: 241 GGEALNNLRALQLTLFKNSSYVGVPFSSLVLYPARTLAAKRLGAQIDRLFFSIISDRKIS 300

Query: 301 SDSD--SDLLSRMIQSQSNQKQGSAAQLTPREVVDECKTFFFGGHETTALAISWTLLLLA 360
                 SDLLSR+I S+        A L+PREVVDECKTFFFGGHETTALAISWTLLLLA
Sbjct: 301 GSGSAYSDLLSRLIGSEYK----GGAGLSPREVVDECKTFFFGGHETTALAISWTLLLLA 360

Query: 361 TRPDWQNILREEIKEVIGDKDIDFSMLSSLKKV 388
           T PDWQ ILR+EIKEVIGDKDIDFSMLSSLKK+
Sbjct: 361 TSPDWQTILRDEIKEVIGDKDIDFSMLSSLKKM 382

[Close]

BLAST of Lsi05G012190 vs. NCBI nr
Match: gi|700202249|gb|KGN57382.1| (hypothetical protein Csa_3G182180 [Cucumis sativus])

HSP 1 Score: 598.6 bits (1542), Expect = 8.1e-168
Identity = 315/384 (82.03%), Postives = 333/384 (86.72%), Query Frame = 1


Query: 20  MVLFVKVVVSWWILPILAHKKLKRNGFEGPSPTFPLGNITEMKKIMKAAAA----INGSS 79
           MVLF KVV+S WILP LAHKKLKRNGFEGPSP+FPLGNITEMKKIMKAAAA    INGSS
Sbjct: 1   MVLFGKVVISCWILPTLAHKKLKRNGFEGPSPSFPLGNITEMKKIMKAAAAGGAAINGSS 60

Query: 80  TTSLDASSNNVSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIADPQLVKKISEAVM 139
                  SNN+SHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIADPQLVKKISEAV+
Sbjct: 61  -------SNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIADPQLVKKISEAVL 120

Query: 140 GKSWGKPTVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPTNLKAMTKFMVESTTKM 199
           GKSWGKP VFRNDREPMFGDGLVMTEGD+WIRHRHILTPAFNP NLKAMTKFMVEST KM
Sbjct: 121 GKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPANLKAMTKFMVESTKKM 180

Query: 200 VKEWRSLVKSGKPEIDVEREITATAGDIIAKASFGIWSGGGGEEALNNLRALQLTLFSNS 259
           ++EWR  VKSG+ EI+VEREITATAG+IIAKASFGI   GGG EALNNLRALQLTLF NS
Sbjct: 181 LEEWRRQVKSGQSEIEVEREITATAGEIIAKASFGISKCGGGGEALNNLRALQLTLFKNS 240

Query: 260 SYVGVPFSSIFLYPARTVAAKRLGAQIDRHFFSIISHRKLSSDSD--SDLLSRMIQSQSN 319
           SYVGVPFSS+ LYPART+AAKRLGAQIDR FFSIIS RK+S      SDLLSR+I S+  
Sbjct: 241 SYVGVPFSSLVLYPARTLAAKRLGAQIDRLFFSIISDRKISGSGSAYSDLLSRLIGSEYK 300

Query: 320 QKQGSAAQLTPREVVDECKTFFFGGHETTALAISWTLLLLATRPDWQNILREEIKEVIGD 379
                 A L+PREVVDECKTFFFGGHETTALAISWTLLLLAT PDWQ ILR+EIKEVIGD
Sbjct: 301 ----GGAGLSPREVVDECKTFFFGGHETTALAISWTLLLLATSPDWQTILRDEIKEVIGD 360

Query: 380 KDIDFSMLSSLKKVS-PYFLFFLF 397
           KDIDFSMLSSLKKV   +FL F F
Sbjct: 361 KDIDFSMLSSLKKVPFSFFLSFFF 373

[Close]

BLAST of Lsi05G012190 vs. NCBI nr
Match: gi|395146476|gb|AFN53633.1| (putative cytochrome P450 protein [Linum usitatissimum])

HSP 1 Score: 438.7 bits (1127), Expect = 1.1e-119
Identity = 218/368 (59.24%), Postives = 281/368 (76.36%), Query Frame = 1


Query: 26  VVVSWWILPILAHKKLKRNGFEGPSPTFPLGNITEMKKIMKAAAAINGSSTTSLDASSNN 85
           +V ++W+ P+ A+ KL+RNGF GP+P+FPLGNI EM+ I K    ++ SS+         
Sbjct: 22  LVYAFWVSPLAAYYKLRRNGFGGPTPSFPLGNIKEMRTIAK--LTVSSSSSGGGCGGGGG 81

Query: 86  VSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIADPQLVKKISEAVMGKSWGKPTVF 145
           +SHDIHS +FP+F+QWQNS+GK FVYWLGTEPF+YIADP+ +K++S  VMGKSWGKP VF
Sbjct: 82  ISHDIHSNVFPYFSQWQNSHGKVFVYWLGTEPFMYIADPEFLKEMSAGVMGKSWGKPRVF 141

Query: 146 RNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPTNLKAMTKFMVESTTKMVKEWRSLVKS 205
           + DREPMFG+GLVM EGDDW+RHRH++TPAF P+NLKAM+  MVESTT+M+ EW +L+ S
Sbjct: 142 KQDREPMFGNGLVMVEGDDWVRHRHVITPAFTPSNLKAMSSLMVESTTRMLDEWATLISS 201

Query: 206 GKPEIDVEREITATAGDIIAKASFGIWSGGGGEEALNNLRALQLTLFSNSSYVGVPFSSI 265
           G PEI+VE  ITATAG+IIAK SFG+     G   +  LRA+QLTLF+++ YVGVP+S I
Sbjct: 202 GVPEIEVESHITATAGEIIAKTSFGVGYNESGRMVVEKLRAMQLTLFNSNRYVGVPYSKI 261

Query: 266 FLYPARTVAAKRLGAQIDRHFFSIISHRKLS----SDSDSDLLSRMIQSQSNQKQGSAAQ 325
            + P +T+ AK+LG +ID    SIIS RK S    ++++ DLL  ++  Q N   G   +
Sbjct: 262 -MCPRKTLDAKKLGKEIDSLLLSIISTRKNSLNTTTNNNKDLLGLLL--QENHADGRLGK 321

Query: 326 -LTPREVVDECKTFFFGGHETTALAISWTLLLLATRPDWQNILREEIKEVIGDKD-IDFS 385
            LT RE+VDECKTFFFGGHETTALAISWTL+LLA  P+WQ+ LR+EIKEV GDK  +DF+
Sbjct: 322 TLTTRELVDECKTFFFGGHETTALAISWTLMLLAKHPEWQDELRKEIKEVSGDKTRVDFA 381

Query: 386 MLSSLKKV 388
           MLSSLKK+
Sbjct: 382 MLSSLKKM 384

[Close]

BLAST of Lsi05G012190 vs. NCBI nr
Match: gi|823214789|ref|XP_012440152.1| (PREDICTED: cytokinin hydroxylase [Gossypium raimondii])

HSP 1 Score: 437.6 bits (1124), Expect = 2.4e-119
Identity = 220/369 (59.62%), Postives = 277/369 (75.07%), Query Frame = 1


Query: 23  FVKVVVSWWILPILAHKKLKRNGFEGPSPTFPLGNITEMKKIMKAAAAINGSSTTSLDAS 82
           F  ++ S W+LPI A+KK+K+NGF GP P+FP+GNITEMK  M     IN SS      S
Sbjct: 23  FSTLIFSCWVLPIRAYKKIKKNGFTGPIPSFPMGNITEMKNSMN----INVSS------S 82

Query: 83  SNNVSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIADPQLVKKISEAVMGKSWGKP 142
            N VSHDIHST+FP+FA+WQ SYGK F+YWLGTEPFLY+A+P+ +K +S  V+GKSWGKP
Sbjct: 83  LNVVSHDIHSTVFPYFARWQKSYGKLFIYWLGTEPFLYVAEPEFLKNMSAGVLGKSWGKP 142

Query: 143 TVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPTNLKAMTKFMVESTTKMVKEWRSL 202
            VF++DREPMFG GLVM EGD+W+RHRH++TPAF+P NLKAM   MVE TTKM+ +W +L
Sbjct: 143 KVFKHDREPMFGSGLVMVEGDEWVRHRHVITPAFSPCNLKAMASLMVEPTTKMLDQWATL 202

Query: 203 VKSGKPEIDVEREITATAGDIIAKASFGIWSGGGGEEALNNLRALQLTLFSNSSYVGVPF 262
           + SG PEIDVEREIT TAG+IIA+ SFG+ S   G +    LRA+Q+TLF+++ YVGVPF
Sbjct: 203 ISSGYPEIDVEREITTTAGEIIARTSFGL-SYQNGNKVFEKLRAMQITLFNSNRYVGVPF 262

Query: 263 SSIFLYPARTVAAKRLGAQIDRHFFSIISHRKLSSD----SDSDLLSRMIQSQSNQKQGS 322
           S  ++ P + + AKRLG +ID+   SII  RK S D       DLL  +++      + S
Sbjct: 263 SK-WMCPKKNLEAKRLGKEIDQLLLSIIDARKKSLDIGTSPQKDLLGLLMEGGLVDGR-S 322

Query: 323 AAQLTPREVVDECKTFFFGGHETTALAISWTLLLLATRPDWQNILREEIKEVIGDKDIDF 382
              LT RE+VDECKTFFFGGHETTALA++WTLLLLAT PDWQN LR+EIK+VIGD +IDF
Sbjct: 323 GKSLTARELVDECKTFFFGGHETTALALTWTLLLLATYPDWQNQLRDEIKQVIGDGEIDF 378

Query: 383 SMLSSLKKV 388
           + L+ LKK+
Sbjct: 383 TKLADLKKM 378

The following BLAST results are available for this feature:

BLAST of Lsi05G012190 vs. Swiss-Prot


Match Name	E-value	Identity	Description
C7351_ARATH	8.9e-50	32.66	Cytokinin hydroxylase OS=Arabidopsis thaliana GN=CYP735A1 PE=1 SV=1	[more]
C7091_ARATH	6.4e-48	34.36	Cytochrome P450 709B1 OS=Arabidopsis thaliana GN=CYP709B1 PE=2 SV=1	[more]
C7352_ARATH	2.7e-46	32.16	Cytokinin hydroxylase OS=Arabidopsis thaliana GN=CYP735A2 PE=1 SV=1	[more]
C14B1_ORYSJ	2.3e-45	29.35	Cytochrome P450 714B1 OS=Oryza sativa subsp. japonica GN=CYP714B1 PE=1 SV=2	[more]
C14B3_MAIZE	8.7e-45	29.64	Cytochrome P450 714B3 OS=Zea mays GN=CYP714B3 PE=2 SV=1	[more]

BLAST of Lsi05G012190 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L9T4_CUCSA	5.6e-168	82.03	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G182180 PE=4 SV=1	[more]
I6XNC3_LINUS	7.5e-120	59.24	Putative cytochrome P450 protein OS=Linum usitatissimum PE=3 SV=1	[more]
A0A0D2UAW4_GOSRA	1.7e-119	59.62	Uncharacterized protein OS=Gossypium raimondii GN=B456_008G276600 PE=3 SV=1	[more]
M5X7L0_PRUPE	2.2e-119	57.75	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa024029mg PE=3 SV=1	[more]
I6YHZ8_LINUS	6.3e-119	58.58	Cytochrome P450 OS=Linum usitatissimum GN=cyp450 PE=3 SV=1	[more]

BLAST of Lsi05G012190 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G52400.1	1.0e-112	52.51	cytochrome P450, family 715, subfamily A, polypeptide 1	[more]
AT5G38450.1	5.0e-51	32.66	cytochrome P450, family 735, subfamily A, polypeptide 1	[more]
AT2G46960.2	3.6e-49	34.36	cytochrome P450, family 709, subfamily B, polypeptide 1	[more]
AT1G67110.1	1.5e-47	32.16	cytochrome P450, family 735, subfamily A, polypeptide 2	[more]
AT2G46950.1	3.5e-44	32.17	cytochrome P450, family 709, subfamily B, polypeptide 2	[more]

BLAST of Lsi05G012190 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659077497\|ref\|XP_008439236.1\|	2.8e-176	83.55	PREDICTED: cytokinin hydroxylase [Cucumis melo]	[more]
gi\|778679591\|ref\|XP_004151435.2\|	7.6e-174	82.70	PREDICTED: cytokinin hydroxylase [Cucumis sativus]	[more]
gi\|700202249\|gb\|KGN57382.1\|	8.1e-168	82.03	hypothetical protein Csa_3G182180 [Cucumis sativus]	[more]
gi\|395146476\|gb\|AFN53633.1\|	1.1e-119	59.24	putative cytochrome P450 protein [Linum usitatissimum]	[more]
gi\|823214789\|ref\|XP_012440152.1\|	2.4e-119	59.62	PREDICTED: cytokinin hydroxylase [Gossypium raimondii]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0020037	heme binding
GO:0005506	iron ion binding
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Vocabulary: **INTERPRO**
Term	Definition
IPR002401	Cyt_P450_E_grp-I
IPR001128	Cyt_P450

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0016020	membrane
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G012190.1	Lsi05G012190.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	GENE3D	G3DSA:1.10.630.10		coord: 31..386 score: 5.9
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 93..385 score: 3.2
IPR001128	Cytochrome P450	unknown	SSF48264	Cytochrome P450	coord: 88..385 score: 9.82
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 102..121 score: 9.6E-10coord: 326..343 score: 9.6E-10coord: 346..372 score: 9.6
None	No IPR available	PANTHER	PTHR24282	CYTOCHROME P450 FAMILY MEMBER	coord: 1..388 score: 3.1E
None	No IPR available	PANTHER	PTHR24282:SF15	CYTOCHROME P450, FAMILY 715, SUBFAMILY A, POLYPEPTIDE 1	coord: 1..388 score: 3.1E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi05G012190	Csa3G182180	Cucumber (Chinese Long) v2	culsiB210
Lsi05G012190	CSPI03G17010	Wild cucumber (PI 183967)	cpilsiB208
Lsi05G012190	Cucsa.107860	Cucumber (Gy14) v1	cgylsiB143
Lsi05G012190	CmaCh18G012500	Cucurbita maxima (Rimu)	cmalsiB387
Lsi05G012190	CmaCh02G010690	Cucurbita maxima (Rimu)	cmalsiB587
Lsi05G012190	CmoCh18G012730	Cucurbita moschata (Rifu)	cmolsiB372
Lsi05G012190	CmoCh02G010870	Cucurbita moschata (Rifu)	cmolsiB567
Lsi05G012190	Cla006173	Watermelon (97103) v1	lsiwmB366
Lsi05G012190	MELO3C006916	Melon (DHL92) v3.5.1	lsimeB379
Lsi05G012190	ClCG05G008110	Watermelon (Charleston Gray)	lsiwcgB336

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi05G012190	Bottle gourd (USVL1VR-Ls)	lsilsiB160
Lsi05G012190	Cucurbita maxima (Rimu)	cmalsiB312
Lsi05G012190	Cucurbita maxima (Rimu)	cmalsiB836
Lsi05G012190	Cucurbita moschata (Rifu)	cmolsiB300
Lsi05G012190	Cucurbita pepo (Zucchini)	cpelsiB035
Lsi05G012190	Cucurbita pepo (Zucchini)	cpelsiB172
Lsi05G012190	Cucurbita pepo (Zucchini)	cpelsiB618
Lsi05G012190	Cucumber (Gy14) v2	cgyblsiB193
Lsi05G012190	Cucumber (Gy14) v2	cgyblsiB197
Lsi05G012190	Melon (DHL92) v3.6.1	lsimedB394
Lsi05G012190	Melon (DHL92) v3.6.1	lsimedB397
Lsi05G012190	Silver-seed gourd	carlsiB650
Lsi05G012190	Silver-seed gourd	carlsiB675
Lsi05G012190	Silver-seed gourd	carlsiB903
Lsi05G012190	Cucumber (Chinese Long) v3	cuclsiB229
Lsi05G012190	Watermelon (97103) v2	lsiwmbB331
Lsi05G012190	Watermelon (97103) v2	lsiwmbB334
Lsi05G012190	Wax gourd	lsiwgoB434
Lsi05G012190	Wax gourd	lsiwgoB443