Lsi05G009010 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi05G009010
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionPR-6 proteinase inhibitor family protein
Locationchr05 : 16079539 .. 16080452 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTACATAGTATTTGTTACTTGTCGTCTAAAAGAATAACAAATACATAGTGTTTTTTTCCAATATTTTTAAATATACTTCTTTTTTGTTTTATCTCATGTAAAATGGTGTTGATAATGACTACTTGTTGGATAATTTTGTTTAGCTTTGGTGGTCAAATTGAGAAACACTAAACTCTACCATTTAAAAAAAAGGAAAATCCCAATGTCTTTCTAAAATCTAGAATAATCTTTTAAGTTTTAGAAGATAAACATAACAATATTCCATTTTAAAAAAAAAAAAAATCTAAATATGATTTTTTAAATAAAGTCCATATTCGTATTTAAAGATTTGAAAGATAAACATAAAAAATGGTAATCTTTTTTTAAATCGTGTTTACAAATTTGAAACATAGACACACATCTTTTTCAAAAAGAAGGAATGCATACGGTTATTCCTTACCATTTTCATTTATTTATTAATCTGGTAGAATTAACCTAACACAATTTATTTTTTATTTTATTGACATCATGTGACCAAACTAACTATAAATATAGCTACACAATCAACAACAAAGCACATCCCACTCAACTTGTAACAAATAGACAGATAACAAGTCCAAGATGACGTATCATTGTAAGTATAGCTTTTTGTAATATTTTGGTTGAGTTCATCTTCCTTTGTAAATATACATTTAATCTTAATTACTAAACAAATTATTTTTGCATATGTTTGAAAATAGTCAAATCATGGCCAGAACTCAACCTCATAGATGCAAACACTGCAGCAAATATCATCAAGAAAGAACATCCTAACTTCAATCCAGTCAAAGTGTTGGCAGGAAGTCCGGTAACACTAGATCTTCGACAAGGTCGAGTTCGGCTATTTGTTAATATAAAGGAAATAGTGGTTGAAGTTCCTCAAGAGGGCTGA

mRNA sequence

ATGGTCAAATCATGGCCAGAACTCAACCTCATAGATGCAAACACTGCAGCAAATATCATCAAGAAAGAACATCCTAACTTCAATCCAGTCAAAGTGTTGGCAGGAAGTCCGGTAACACTAGATCTTCGACAAGGTCGAGTTCGGCTATTTGTTAATATAAAGGAAATAGTGGTTGAAGTTCCTCAAGAGGGCTGA

Coding sequence (CDS)

ATGGTCAAATCATGGCCAGAACTCAACCTCATAGATGCAAACACTGCAGCAAATATCATCAAGAAAGAACATCCTAACTTCAATCCAGTCAAAGTGTTGGCAGGAAGTCCGGTAACACTAGATCTTCGACAAGGTCGAGTTCGGCTATTTGTTAATATAAAGGAAATAGTGGTTGAAGTTCCTCAAGAGGGCTGA

Protein sequence

MVKSWPELNLIDANTAANIIKKEHPNFNPVKVLAGSPVTLDLRQGRVRLFVNIKEIVVEVPQEG
Homology
[Close]
BLAST of Lsi05G009010 vs. Swiss-Prot
Match: IPIB_TOBAC (Proteinase inhibitor I-B OS=Nicotiana tabacum GN=TIMPA PE=2 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 7.9e-09
Identity = 33/61 (54.10%), Postives = 40/61 (65.57%), Query Frame = 1 

Query: 5   WPELNLIDANTAANIIKKEHPNFNPVK-VLAGSPVTLDLRQGRVRLFVNIKEIVVEVPQE 64
           WPEL    A  A  II+KE+P    V+ VL G+PVT DLR  RVRLFVN+ + VV+ PQ 
Sbjct: 47  WPELLGTPAKFAMQIIQKENPKLTNVQTVLNGTPVTEDLRCNRVRLFVNVLDFVVQTPQV 106

[Close]
BLAST of Lsi05G009010 vs. Swiss-Prot
Match: IPIA_TOBAC (Proteinase inhibitor I-A OS=Nicotiana tabacum GN=TIMPB PE=2 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 2.3e-08
Identity = 31/61 (50.82%), Postives = 39/61 (63.93%), Query Frame = 1 

Query: 5   WPELNLIDANTAANIIKKEHPNFNPVK-VLAGSPVTLDLRQGRVRLFVNIKEIVVEVPQE 64
           WPEL    A  A  II+KE+P    V+ +L G PVT DLR  RVRLFVN+ + +V+ PQ 
Sbjct: 47  WPELLGTPAKFAMQIIQKENPKLTNVQTILNGGPVTEDLRCNRVRLFVNVLDFIVQTPQI 106

[Close]
BLAST of Lsi05G009010 vs. Swiss-Prot
Match: ITR1_NICSY (Trypsin inhibitor 1 OS=Nicotiana sylvestris PE=2 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 3.9e-08
Identity = 31/63 (49.21%), Postives = 41/63 (65.08%), Query Frame = 1 

Query: 3  KSWPELNLIDANTAANIIKKEHPNFNPV-KVLAGSPVTLDLRQGRVRLFVNIKEIVVEVP 62
          ++WPEL  + A  A  II+KE+     V  VL GSPVT D R  RVRLFVN+ + VV++P
Sbjct: 32 ETWPELIGVPAKFAREIIQKENSKLTNVPSVLNGSPVTKDFRCERVRLFVNVLDFVVQIP 91

Query: 63 QEG 65
          + G
Sbjct: 92 RVG 94

[Close]
BLAST of Lsi05G009010 vs. Swiss-Prot
Match: IER1_SOLLC (Ethylene-responsive proteinase inhibitor 1 OS=Solanum lycopersicum PE=3 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 8.8e-08
Identity = 32/63 (50.79%), Postives = 40/63 (63.49%), Query Frame = 1 

Query: 3   KSWPELNLIDANTAANIIKKEHPNFNPVKVLA-GSPVTLDLRQGRVRLFVNIKEIVVEVP 62
           +SWPEL    A  A  II+KE+P    V+ L  GS  T DLR  RVRLFVN+ +IVV+ P
Sbjct: 57  ESWPELLGTPAKFAKQIIQKENPKLTNVETLLNGSAFTEDLRCNRVRLFVNLLDIVVQTP 116

Query: 63  QEG 65
           + G
Sbjct: 117 KVG 119

[Close]
BLAST of Lsi05G009010 vs. Swiss-Prot
Match: ICI1_SOLPE (Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum PE=3 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 3.3e-07
Identity = 30/59 (50.85%), Postives = 39/59 (66.10%), Query Frame = 1 

Query: 5   WPELNLIDANTAANIIKKEHPNFNPVKVLA-GSPVTLDLRQGRVRLFVNIKEIVVEVPQ 63
           WPEL  + A  A  II+KE+P+   + +L  GSPVT D R  RVRLFVNI   VV++P+
Sbjct: 51  WPELIGVPALYAKGIIEKENPSITNIPILLNGSPVTKDFRCDRVRLFVNILGDVVQIPR 109

[Close]
BLAST of Lsi05G009010 vs. TrEMBL
Match: A0A0A0LIF6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G914570 PE=4 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 1.4e-15
Identity = 43/63 (68.25%), Postives = 51/63 (80.95%), Query Frame = 1 

Query: 2  VKSWPELNLIDANTAANIIKKEHPNFNPVKVLAGSPVTLDLRQGRVRLFVNIKEIVVEVP 61
          +KSWP LN +DA T ANIIKKE+P+F    +LAGSPVT DL+ GRVRLF N++EIVV VP
Sbjct: 9  LKSWPALNFMDARTVANIIKKENPDFQIFILLAGSPVTKDLKHGRVRLFTNMEEIVVIVP 68

Query: 62 QEG 65
          QEG
Sbjct: 69 QEG 71

[Close]
BLAST of Lsi05G009010 vs. TrEMBL
Match: A0A0A0LFD4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G914580 PE=4 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 2.9e-10
Identity = 36/63 (57.14%), Postives = 42/63 (66.67%), Query Frame = 1 

Query: 2  VKSWPELNLIDANTAANIIKKEHPNFNPVKVLAGSPVTLDLRQGRVRLFVNIKEIVVEVP 61
          +KSWP L  +DA T AN IK E+PNF  V V  G+PVT D+  GRVRL V+    VV VP
Sbjct: 7  LKSWPALKFLDAQTVANAIKTENPNFEIVTVPEGTPVTKDIIPGRVRLVVDANGKVVNVP 66

Query: 62 QEG 65
          QEG
Sbjct: 67 QEG 69

[Close]
BLAST of Lsi05G009010 vs. TrEMBL
Match: A0A0A0KPI0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G027950 PE=4 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 1.1e-09
Identity = 32/61 (52.46%), Postives = 45/61 (73.77%), Query Frame = 1 

Query: 4  SWPELNLIDANTAANIIKKEHPNFNPVKVLAGSPVTLDLRQGRVRLFVNIKEIVVEVPQE 63
          SWPEL  + ++ A + I+++ P+  PV +LAGSPVT DLR  RVR+FVN+ + VVEVP+ 
Sbjct: 15 SWPELVFVKSSVAVHWIERDRPDVKPVVLLAGSPVTEDLRPNRVRIFVNMNDRVVEVPRT 74

Query: 64 G 65
          G
Sbjct: 75 G 75

[Close]
BLAST of Lsi05G009010 vs. TrEMBL
Match: Q40444_9SOLA (Tumor-related protein (Fragment) OS=Nicotiana glauca x Nicotiana langsdorffii PE=2 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 1.4e-07
Identity = 32/63 (50.79%), Postives = 43/63 (68.25%), Query Frame = 1 

Query: 3  KSWPELNLIDANTAANIIKKEHPNFNPV-KVLAGSPVTLDLRQGRVRLFVNIKEIVVEVP 62
          ++WPEL  + A  A  II+KE+P    V  VL GSPVT D R  RVRLFVN+ ++VV++P
Sbjct: 2  ETWPELIGVPAKFAREIIQKENPKLTNVPSVLNGSPVTKDFRCNRVRLFVNVLDVVVQIP 61

Query: 63 QEG 65
          + G
Sbjct: 62 RVG 64

[Close]
BLAST of Lsi05G009010 vs. TrEMBL
Match: A0A0J8CE73_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_5g120640 PE=4 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 3.0e-07
Identity = 30/58 (51.72%), Postives = 39/58 (67.24%), Query Frame = 1 

Query: 4  SWPELNLIDANTAANIIKKEHPNFNPVKVLAGSPVTLDLRQGRVRLFVNIKEIVVEVP 62
          +WPEL       AA II+KE+PN   + +L G+PVTLD R  RVR+FV+ K+IVV  P
Sbjct: 9  TWPELVGEKGQKAAEIIEKENPNVKAIVILDGTPVTLDFRCDRVRVFVDCKDIVVRAP 66

[Close]
BLAST of Lsi05G009010 vs. TAIR10
Match: AT2G38870.1 (AT2G38870.1 Serine protease inhibitor, potato inhibitor I-type family protein)

HSP 1 Score: 60.5 bits (145), Expect = 4.5e-10
Identity = 29/61 (47.54%), Postives = 40/61 (65.57%), Query Frame = 1 

Query: 4  SWPELNLIDANTAANIIKKEHPNFNPVKVLAGSPVTLDLRQGRVRLFVNIKEIVVEVPQE 63
          SWPEL   + + AA +I++E+P  N   +L GSPVT D R  RVR+FV+   IVV+ P+ 
Sbjct: 10 SWPELTGTNGDYAAVVIERENPTVNAAVILDGSPVTADFRCDRVRVFVDGNRIVVKTPKS 69

Query: 64 G 65
          G
Sbjct: 70 G 70

[Close]
BLAST of Lsi05G009010 vs. NCBI nr
Match: gi|700205344|gb|KGN60477.1| (hypothetical protein Csa_3G914570 [Cucumis sativus])

HSP 1 Score: 89.7 bits (221), Expect = 2.0e-15
Identity = 43/63 (68.25%), Postives = 51/63 (80.95%), Query Frame = 1 

Query: 2  VKSWPELNLIDANTAANIIKKEHPNFNPVKVLAGSPVTLDLRQGRVRLFVNIKEIVVEVP 61
          +KSWP LN +DA T ANIIKKE+P+F    +LAGSPVT DL+ GRVRLF N++EIVV VP
Sbjct: 9  LKSWPALNFMDARTVANIIKKENPDFQIFILLAGSPVTKDLKHGRVRLFTNMEEIVVIVP 68

Query: 62 QEG 65
          QEG
Sbjct: 69 QEG 71

[Close]
BLAST of Lsi05G009010 vs. NCBI nr
Match: gi|700205345|gb|KGN60478.1| (hypothetical protein Csa_3G914580 [Cucumis sativus])

HSP 1 Score: 72.0 bits (175), Expect = 4.2e-10
Identity = 36/63 (57.14%), Postives = 42/63 (66.67%), Query Frame = 1 

Query: 2  VKSWPELNLIDANTAANIIKKEHPNFNPVKVLAGSPVTLDLRQGRVRLFVNIKEIVVEVP 61
          +KSWP L  +DA T AN IK E+PNF  V V  G+PVT D+  GRVRL V+    VV VP
Sbjct: 7  LKSWPALKFLDAQTVANAIKTENPNFEIVTVPEGTPVTKDIIPGRVRLVVDANGKVVNVP 66

Query: 62 QEG 65
          QEG
Sbjct: 67 QEG 69

[Close]
BLAST of Lsi05G009010 vs. NCBI nr
Match: gi|778698123|ref|XP_011654473.1| (PREDICTED: inhibitor of trypsin and hageman factor-like [Cucumis sativus])

HSP 1 Score: 70.1 bits (170), Expect = 1.6e-09
Identity = 32/61 (52.46%), Postives = 45/61 (73.77%), Query Frame = 1 

Query: 4  SWPELNLIDANTAANIIKKEHPNFNPVKVLAGSPVTLDLRQGRVRLFVNIKEIVVEVPQE 63
          SWPEL  + ++ A + I+++ P+  PV +LAGSPVT DLR  RVR+FVN+ + VVEVP+ 
Sbjct: 15 SWPELVFVKSSVAVHWIERDRPDVKPVVLLAGSPVTEDLRPNRVRIFVNMNDRVVEVPRT 74

Query: 64 G 65
          G
Sbjct: 75 G 75

[Close]
BLAST of Lsi05G009010 vs. NCBI nr
Match: gi|685298675|ref|XP_009140106.1| (PREDICTED: protease inhibitor HPI-like [Brassica rapa])

HSP 1 Score: 64.3 bits (155), Expect = 8.8e-08
Identity = 31/63 (49.21%), Postives = 44/63 (69.84%), Query Frame = 1 

Query: 2  VKSWPELNLIDANTAANIIKKEHPNFNPVKVLAGSPVTLDLRQGRVRLFVNIKEIVVEVP 61
          +K+WPEL     + AA++IK+E+P+FN   +L GS VT DLR+ RVR++V+   IVV VP
Sbjct: 3  LKTWPELVGTHGDDAASVIKRENPSFNVFVLLIGSIVTADLRENRVRVWVDTNRIVVRVP 62

Query: 62 QEG 65
            G
Sbjct: 63 TTG 65

[Close]
BLAST of Lsi05G009010 vs. NCBI nr
Match: gi|688425|dbj|BAA05472.1| (tumor-related protein [Nicotiana glauca x Nicotiana langsdorffii])

HSP 1 Score: 63.2 bits (152), Expect = 2.0e-07
Identity = 32/63 (50.79%), Postives = 43/63 (68.25%), Query Frame = 1 

Query: 3  KSWPELNLIDANTAANIIKKEHPNFNPV-KVLAGSPVTLDLRQGRVRLFVNIKEIVVEVP 62
          ++WPEL  + A  A  II+KE+P    V  VL GSPVT D R  RVRLFVN+ ++VV++P
Sbjct: 2  ETWPELIGVPAKFAREIIQKENPKLTNVPSVLNGSPVTKDFRCNRVRLFVNVLDVVVQIP 61

Query: 63 QEG 65
          + G
Sbjct: 62 RVG 64

The following BLAST results are available for this feature:
BLAST of Lsi05G009010 vs. Swiss-Prot
Match NameE-valueIdentityDescription
IPIB_TOBAC7.9e-0954.10Proteinase inhibitor I-B OS=Nicotiana tabacum GN=TIMPA PE=2 SV=1[more]
IPIA_TOBAC2.3e-0850.82Proteinase inhibitor I-A OS=Nicotiana tabacum GN=TIMPB PE=2 SV=1[more]
ITR1_NICSY3.9e-0849.21Trypsin inhibitor 1 OS=Nicotiana sylvestris PE=2 SV=1[more]
IER1_SOLLC8.8e-0850.79Ethylene-responsive proteinase inhibitor 1 OS=Solanum lycopersicum PE=3 SV=1[more]
ICI1_SOLPE3.3e-0750.85Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum PE=3 SV=1[more]
BLAST of Lsi05G009010 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LIF6_CUCSA1.4e-1568.25Uncharacterized protein OS=Cucumis sativus GN=Csa_3G914570 PE=4 SV=1[more]
A0A0A0LFD4_CUCSA2.9e-1057.14Uncharacterized protein OS=Cucumis sativus GN=Csa_3G914580 PE=4 SV=1[more]
A0A0A0KPI0_CUCSA1.1e-0952.46Uncharacterized protein OS=Cucumis sativus GN=Csa_5G027950 PE=4 SV=1[more]
Q40444_9SOLA1.4e-0750.79Tumor-related protein (Fragment) OS=Nicotiana glauca x Nicotiana langsdorffii PE... [more]
A0A0J8CE73_BETVU3.0e-0751.72Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_5g120640 PE=4 S... [more]
BLAST of Lsi05G009010 vs. TAIR10
Match NameE-valueIdentityDescription
AT2G38870.14.5e-1047.54 Serine protease inhibitor, potato inhibitor I-type family protein[more]
BLAST of Lsi05G009010 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|700205344|gb|KGN60477.1|2.0e-1568.25hypothetical protein Csa_3G914570 [Cucumis sativus][more]
gi|700205345|gb|KGN60478.1|4.2e-1057.14hypothetical protein Csa_3G914580 [Cucumis sativus][more]
gi|778698123|ref|XP_011654473.1|1.6e-0952.46PREDICTED: inhibitor of trypsin and hageman factor-like [Cucumis sativus][more]
gi|685298675|ref|XP_009140106.1|8.8e-0849.21PREDICTED: protease inhibitor HPI-like [Brassica rapa][more]
gi|688425|dbj|BAA05472.1|2.0e-0750.79tumor-related protein [Nicotiana glauca x Nicotiana langsdorffii][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0009611response to wounding
Vocabulary: Molecular Function
TermDefinition
GO:0004867serine-type endopeptidase inhibitor activity
Vocabulary: INTERPRO
TermDefinition
IPR000864Prot_inh_pot1
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0050832 defense response to fungus
biological_process GO:0010951 negative regulation of endopeptidase activity
biological_process GO:0009611 response to wounding
cellular_component GO:0005618 cell wall
cellular_component GO:0005575 cellular_component
molecular_function GO:0004867 serine-type endopeptidase inhibitor activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G009010.1Lsi05G009010.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000864Proteinase inhibitor I13, potato inhibitor IPRODOMPD002604coord: 5..64
score: 3.
IPR000864Proteinase inhibitor I13, potato inhibitor IPRINTSPR00292POTATOINHBTRcoord: 41..51
score: 2.9E-5coord: 4..17
score: 2.9E-5coord: 29..40
score: 2.
IPR000864Proteinase inhibitor I13, potato inhibitor IPFAMPF00280potato_inhibitcoord: 4..63
score: 2.0
IPR000864Proteinase inhibitor I13, potato inhibitor IunknownSSF54654CI-2 family of serine protease inhibitorscoord: 3..64
score: 4.84
NoneNo IPR availableGENE3DG3DSA:3.30.10.10coord: 3..64
score: 7.4
NoneNo IPR availablePANTHERPTHR33091FAMILY NOT NAMEDcoord: 4..64
score: 4.4
NoneNo IPR availablePANTHERPTHR33091:SF1SUBFAMILY NOT NAMEDcoord: 4..64
score: 4.4

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Lsi05G009010Csa3G914570Cucumber (Chinese Long) v2culsiB221
Lsi05G009010CSPI03G47610Wild cucumber (PI 183967)cpilsiB222
Lsi05G009010Cucsa.313930Cucumber (Gy14) v1cgylsiB507
Lsi05G009010ClCG09G013040Watermelon (Charleston Gray)lsiwcgB316
Lsi05G009010Cla97C09G175800Watermelon (97103) v2lsiwmbB354
Lsi05G009010Cp4.1LG15g00590Cucurbita pepo (Zucchini)cpelsiB211
Lsi05G009010CsGy3G044950Cucumber (Gy14) v2cgyblsiB208
Lsi05G009010CsaV3_3G050000Cucumber (Chinese Long) v3cuclsiB240
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Lsi05G009010Wax gourdlsiwgoB459
Lsi05G009010Wild cucumber (PI 183967)cpilsiB466
Lsi05G009010Cucumber (Chinese Long) v2culsiB459
Lsi05G009010Cucumber (Gy14) v2cgyblsiB427
Lsi05G009010Cucumber (Chinese Long) v3cuclsiB488