Lsi05G008440 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi05G008440
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionPolygalacturonase
Locationchr05 : 13864169 .. 13864705 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAAGAAAATGGTGTTCAAAATGTAACAATGAAATCTGCTACATTCACCAACACTCAAAATGGGGTTAGAATTAAAACATGGGGAAGGCCAAGCAAAGGGTTTGCAAAAAACATTCTTTTTCAAGACATTGTAATGGTTAATGTCGAAAATCCTATCATTATCGATCAAAATTATTGCCCCGACAACAAAGGTTGTCTTGGACAAGTCCGTATCGACATTTTTTTTGGTTATCCAACCTCAGCTTGCCACGTGCCAAACTATTTTTTTTTCTTTTAAATATTTTGTATCTTATATATTTATTTTTTTTTCAAATAGGATTATGGAGTTAAAATTAGCGATGTGACATATCGAAACATCCATGGAACATCAGCTACACAAGTTGCGATGAAATTCGATTGTAGCTCAAAATTTCCATGCAGTAACATAACGCTGGAGGATGTGAAGCTGAATTATAAAAATGAAGCAGCTAAAGCTTCATATAGTCATGCTGAAGGAAGTGCTGCTCGTTTAGTTCAACCATCAAGTTGTTTGTAG

mRNA sequence

ATGCAAGAAAATGGTGTTCAAAATGTAACAATGAAATCTGCTACATTCACCAACACTCAAAATGGGGTTAGAATTAAAACATGGGGAAGGCCAAGCAAAGGGTTTGCAAAAAACATTCTTTTTCAAGACATTGTAATGGTTAATGTCGAAAATCCTATCATTATCGATCAAAATTATTGCCCCGACAACAAAGGTTGTCTTGGACAAGATTATGGAGTTAAAATTAGCGATGTGACATATCGAAACATCCATGGAACATCAGCTACACAAGTTGCGATGAAATTCGATTGTAGCTCAAAATTTCCATGCAGTAACATAACGCTGGAGGATGTGAAGCTGAATTATAAAAATGAAGCAGCTAAAGCTTCATATAGTCATGCTGAAGGAAGTGCTGCTCGTTTAGTTCAACCATCAAGTTGTTTGTAG

Coding sequence (CDS)

ATGCAAGAAAATGGTGTTCAAAATGTAACAATGAAATCTGCTACATTCACCAACACTCAAAATGGGGTTAGAATTAAAACATGGGGAAGGCCAAGCAAAGGGTTTGCAAAAAACATTCTTTTTCAAGACATTGTAATGGTTAATGTCGAAAATCCTATCATTATCGATCAAAATTATTGCCCCGACAACAAAGGTTGTCTTGGACAAGATTATGGAGTTAAAATTAGCGATGTGACATATCGAAACATCCATGGAACATCAGCTACACAAGTTGCGATGAAATTCGATTGTAGCTCAAAATTTCCATGCAGTAACATAACGCTGGAGGATGTGAAGCTGAATTATAAAAATGAAGCAGCTAAAGCTTCATATAGTCATGCTGAAGGAAGTGCTGCTCGTTTAGTTCAACCATCAAGTTGTTTGTAG

Protein sequence

MQENGVQNVTMKSATFTNTQNGVRIKTWGRPSKGFAKNILFQDIVMVNVENPIIIDQNYCPDNKGCLGQDYGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSNITLEDVKLNYKNEAAKASYSHAEGSAARLVQPSSCL
Homology
[Close]
BLAST of Lsi05G008440 vs. Swiss-Prot
Match: PGLR_PRUPE (Polygalacturonase OS=Prunus persica PE=2 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 6.1e-54
Identity = 99/140 (70.71%), Postives = 119/140 (85.00%), Query Frame = 1 

Query: 2   QENGVQNVTMKSATFTNTQNGVRIKTWGRPSKGFAKNILFQDIVMVNVENPIIIDQNYCP 61
           +E GVQNVT+K+ TF+ TQNG+RIK+WGRPS GFA+NILFQ   MVNVENPI+IDQ+YCP
Sbjct: 254 EEAGVQNVTVKTVTFSGTQNGLRIKSWGRPSTGFARNILFQHATMVNVENPIVIDQHYCP 313

Query: 62  DNKGCLGQDYGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSNITLEDVKLNYKNEAAK 121
           DNKGC GQ  GV+ISDVTY +IHGTSAT+VA+KFDCS K PC  I LEDVKL YKN+AA+
Sbjct: 314 DNKGCPGQVSGVQISDVTYEDIHGTSATEVAVKFDCSPKHPCREIKLEDVKLTYKNQAAE 373

Query: 122 ASYSHAEGSAARLVQPSSCL 142
           +S SHA+G+   +VQP+SCL
Sbjct: 374 SSCSHADGTTEGVVQPTSCL 393

[Close]
BLAST of Lsi05G008440 vs. Swiss-Prot
Match: PGLR6_ARATH (Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana GN=At2g43860 PE=2 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 3.0e-32
Identity = 72/140 (51.43%), Postives = 96/140 (68.57%), Query Frame = 1 

Query: 2   QENGVQNVTMKSATFTNTQNGVRIKTWGRPSKGFAKNILFQDIVMVNVENPIIIDQNYCP 61
           +E GV NVT+ +  F  T NGVRIKTWG+ S  FA+NI+FQ I M  V+NPIIIDQ+YC 
Sbjct: 260 EEQGVDNVTVSNVDFMGTNNGVRIKTWGKDSNSFARNIVFQHINMKMVKNPIIIDQHYCL 319

Query: 62  DNKGCLGQDYGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSNITLEDVKLNYKNEAAK 121
            +K C  Q+ GVK+S+V Y +IHGTS T+VA+  DCS + PC+ I ++DV L   +  A+
Sbjct: 320 -HKPCPKQESGVKVSNVRYEDIHGTSNTEVAVLLDCSKEKPCTGIVMDDVNLVSVHRPAQ 379

Query: 122 ASYSHAEGSAARLVQPSSCL 142
           AS  +A GSA  +V  + CL
Sbjct: 380 ASCDNANGSANDVVPFTPCL 398

[Close]
BLAST of Lsi05G008440 vs. Swiss-Prot
Match: PGLR_ACTDE (Polygalacturonase OS=Actinidia deliciosa PE=2 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 2.2e-27
Identity = 67/136 (49.26%), Postives = 84/136 (61.76%), Query Frame = 1 

Query: 3   ENGVQNVTMKSATFTNTQNGVRIKTWGRPSKGFAKNILFQDIVMVNVENPIIIDQNYCPD 62
           E  V +V +  A    T NGVRIKTW +   G A NI FQ++ M NVENPIIIDQNYC  
Sbjct: 319 EAHVSDVVVNGAKLCGTTNGVRIKTW-QGGSGSASNIKFQNVEMHNVENPIIIDQNYCDQ 378

Query: 63  NKGCLGQDYGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSNITLEDVKLNYK-NEAAK 122
           +K C  Q   V++ +V Y+NI GT A+ VA+ FDCS +FPC  I LEDV L  +   AAK
Sbjct: 379 DKPCQEQSSAVQVKNVVYQNIKGTCASNVAITFDCSKRFPCQGIVLEDVDLEIEGGAAAK 438

Query: 123 ASYSHAEGSAARLVQP 138
           A  ++ E S   +V P
Sbjct: 439 ALCNNVELSETGVVSP 453

[Close]
BLAST of Lsi05G008440 vs. Swiss-Prot
Match: QRT2_ARATH (Polygalacturonase QRT2 OS=Arabidopsis thaliana GN=QRT2 PE=1 SV=2)

HSP 1 Score: 117.9 bits (294), Expect = 9.2e-26
Identity = 62/134 (46.27%), Postives = 85/134 (63.43%), Query Frame = 1 

Query: 6   VQNVTMKSATFTNTQNGVRIKTWGRPSKGFAKNILFQDIVMVNVENPIIIDQNYCPDNKG 65
           V NV +  AT   T NGVRIKTW +   G AKNI+FQDI+M NV NPIII+Q+YC   + 
Sbjct: 304 VSNVVVNKATLIGTTNGVRIKTW-QGGHGMAKNIIFQDIIMKNVTNPIIINQDYCDRVEA 363

Query: 66  CLGQDYGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSNITLEDVKL--NYKNEAAKAS 125
           C  Q   V++S+V Y+NI GTS+  +A+KF CS   PC  I++++VKL    + + +KAS
Sbjct: 364 CPEQKSAVQVSNVLYKNIQGTSSRPIAVKFVCSKNIPCRGISMQNVKLVDQTQQDVSKAS 423

Query: 126 YSHAEGSAARLVQP 138
            S+ +      V P
Sbjct: 424 CSNVKLDTRGNVSP 436

[Close]
BLAST of Lsi05G008440 vs. Swiss-Prot
Match: PGLR2_ARATH (Exopolygalacturonase clone GBGA483 OS=Arabidopsis thaliana GN=At3g07850 PE=2 SV=2)

HSP 1 Score: 114.8 bits (286), Expect = 7.8e-25
Identity = 63/140 (45.00%), Postives = 83/140 (59.29%), Query Frame = 1 

Query: 3   ENGVQNVTMKSATFTNTQNGVRIKTWGRPSKGFAKNILFQDIVMVNVENPIIIDQNYCPD 62
           E  V+ VT++     NT NGVRIKTW     G A NILF+DI M NV  P++IDQ YCP 
Sbjct: 297 EQPVKGVTVRKCLIKNTDNGVRIKTWPGSPPGIASNILFEDITMDNVSLPVLIDQEYCPY 356

Query: 63  NKGCLGQDYGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSNITLEDVKL--NYKNEAA 122
                G    VK+SDVT + I GTSAT+VA+K  CS   PC+NI L D+ L  N K   A
Sbjct: 357 GHCKAGVPSQVKLSDVTIKGIKGTSATKVAVKLMCSKGVPCTNIALSDINLVHNGKEGPA 416

Query: 123 KASYSHAEGSAARLVQPSSC 141
            ++ S+ +   +  + P++C
Sbjct: 417 VSACSNIKPILSGKLVPAAC 436

[Close]
BLAST of Lsi05G008440 vs. TrEMBL
Match: A0A0A0M095_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G627460 PE=3 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 5.4e-65
Identity = 123/141 (87.23%), Postives = 135/141 (95.74%), Query Frame = 1 

Query: 1   MQENGVQNVTMKSATFTNTQNGVRIKTWGRPSKGFAKNILFQDIVMVNVENPIIIDQNYC 60
           +QE+GV+NVT+KSATFTNTQNGVRIKTWG+PS GFA++++FQDIVMVNVENPIIIDQNYC
Sbjct: 246 VQEDGVENVTVKSATFTNTQNGVRIKTWGKPSNGFARSVIFQDIVMVNVENPIIIDQNYC 305

Query: 61  PDNKGCLGQDYGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSNITLEDVKLNYKNEAA 120
           PDNKGC GQD GVKISDVTYRNIHGTSATQVAMKFDCSSKFPCS+ITLEDVKL+ KNEAA
Sbjct: 306 PDNKGCPGQDSGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSDITLEDVKLSCKNEAA 365

Query: 121 KASYSHAEGSAARLVQPSSCL 142
           +AS SHAEGSAA LVQPSSCL
Sbjct: 366 EASCSHAEGSAAGLVQPSSCL 386

[Close]
BLAST of Lsi05G008440 vs. TrEMBL
Match: V7CKC9_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_002G100400g PE=3 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 5.1e-55
Identity = 103/139 (74.10%), Postives = 120/139 (86.33%), Query Frame = 1 

Query: 2   QENGVQNVTMKSATFTNTQNGVRIKTWGRPSKGFAKNILFQDIVMVNVENPIIIDQNYCP 61
           +E GVQNVT+K+ TFT TQNGVRIKTWGRPS GF +N+LFQD +MVNVENP+IIDQNYCP
Sbjct: 258 REAGVQNVTVKTVTFTGTQNGVRIKTWGRPSDGFVRNVLFQDAIMVNVENPVIIDQNYCP 317

Query: 62  DNKGCLGQDYGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSNITLEDVKLNYKNEAAK 121
           DNKGC GQ  GVK+SDVTY++IHGTSATQVA+KFDCSSK+PCS I LEDVKL YKN+ A 
Sbjct: 318 DNKGCPGQASGVKVSDVTYQDIHGTSATQVAVKFDCSSKYPCSRIRLEDVKLTYKNQRAL 377

Query: 122 ASYSHAEGSAARLVQPSSC 141
           +S +HA+G+A   VQP SC
Sbjct: 378 SSCNHADGAALGSVQPESC 396

[Close]
BLAST of Lsi05G008440 vs. TrEMBL
Match: W9RN73_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_020358 PE=3 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 8.6e-55
Identity = 103/141 (73.05%), Postives = 125/141 (88.65%), Query Frame = 1 

Query: 1   MQENGVQNVTMKSATFTNTQNGVRIKTWGRPSKGFAKNILFQDIVMVNVENPIIIDQNYC 60
           ++E GVQNVT+K+A+F NTQNG+RIK+WGRPS GFA+NILFQ  VM NV+NPI+IDQNYC
Sbjct: 262 LKEAGVQNVTVKTASFRNTQNGLRIKSWGRPSSGFARNILFQHAVMTNVQNPIVIDQNYC 321

Query: 61  PDNKGCLGQDYGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSNITLEDVKLNYKNEAA 120
           PDNKGC GQ  GVK+SDVTY++IHGTSAT+VA+KFDCSSK+PC+NI L+DVKL YKN  A
Sbjct: 322 PDNKGCPGQVSGVKVSDVTYQDIHGTSATEVAVKFDCSSKYPCNNIRLQDVKLTYKNLPA 381

Query: 121 KASYSHAEGSAARLVQPSSCL 142
           +AS +HA G+A+ LVQPSSCL
Sbjct: 382 QASCNHAAGTASGLVQPSSCL 402

[Close]
BLAST of Lsi05G008440 vs. TrEMBL
Match: M5WQ98_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa021953mg PE=3 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 1.5e-54
Identity = 104/140 (74.29%), Postives = 124/140 (88.57%), Query Frame = 1 

Query: 2   QENGVQNVTMKSATFTNTQNGVRIKTWGRPSKGFAKNILFQDIVMVNVENPIIIDQNYCP 61
           +E GVQNVT+KS TF NTQNG+RIK+WGRPS GFAK+ILFQ  VM+NV+NPI+IDQNYCP
Sbjct: 237 EEAGVQNVTVKSITFFNTQNGLRIKSWGRPSTGFAKDILFQHAVMLNVQNPIVIDQNYCP 296

Query: 62  DNKGCLGQDYGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSNITLEDVKLNYKNEAAK 121
           DNKGC GQ  GVKISDVTY++IHGTSAT+VA+KFDCSSK+PCSNI ++DVKL YKN+AA+
Sbjct: 297 DNKGCPGQVSGVKISDVTYQDIHGTSATEVAVKFDCSSKYPCSNIKMKDVKLTYKNQAAE 356

Query: 122 ASYSHAEGSAARLVQPSSCL 142
           AS SHA G++   VQP+SCL
Sbjct: 357 ASCSHAAGTSHGTVQPTSCL 376

[Close]
BLAST of Lsi05G008440 vs. TrEMBL
Match: A0A151RM69_CAJCA (Polygalacturonase OS=Cajanus cajan GN=KK1_034877 PE=3 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 1.9e-54
Identity = 102/141 (72.34%), Postives = 120/141 (85.11%), Query Frame = 1 

Query: 1   MQENGVQNVTMKSATFTNTQNGVRIKTWGRPSKGFAKNILFQDIVMVNVENPIIIDQNYC 60
           ++E GVQNVT+K+ TFT TQNGVRIKTWGRPS GF +N+LFQD +MVNV+NP+IIDQNYC
Sbjct: 230 LKEAGVQNVTVKTVTFTGTQNGVRIKTWGRPSTGFVRNVLFQDAIMVNVQNPVIIDQNYC 289

Query: 61  PDNKGCLGQDYGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSNITLEDVKLNYKNEAA 120
           PDNKGC GQ  GVK+SDVTY++IHGTSATQVA+KFDCSSKFPCS I +EDVKL YKN+ A
Sbjct: 290 PDNKGCPGQASGVKVSDVTYQDIHGTSATQVAVKFDCSSKFPCSRIRMEDVKLTYKNQPA 349

Query: 121 KASYSHAEGSAARLVQPSSCL 142
            +S +HA G+    VQP SCL
Sbjct: 350 ISSCNHAVGATLGSVQPQSCL 370

[Close]
BLAST of Lsi05G008440 vs. TAIR10
Match: AT3G59850.1 (AT3G59850.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 184.1 bits (466), Expect = 5.9e-47
Identity = 87/139 (62.59%), Postives = 107/139 (76.98%), Query Frame = 1 

Query: 3   ENGVQNVTMKSATFTNTQNGVRIKTWGRPSKGFAKNILFQDIVMVNVENPIIIDQNYCPD 62
           E GVQN+T+K+ATFT T+NGVRIK+W RPS GFAKNI FQ  VM NV+NPI+IDQNYCP 
Sbjct: 250 EVGVQNITVKTATFTGTENGVRIKSWARPSNGFAKNIRFQHCVMNNVQNPIVIDQNYCPG 309

Query: 63  NKGCLGQDYGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSNITLEDVKLNYKNEAAKA 122
           N+ C  Q  G+KISDV + +IHGTSAT+V +K DCSSK PC+ I ++DVKL Y+N+ A  
Sbjct: 310 NENCPNQVSGIKISDVMFFDIHGTSATEVGVKLDCSSKKPCTGIRIQDVKLTYRNKPATT 369

Query: 123 SYSHAEGSAARLVQPSSCL 142
             SHA GS A   +P+SCL
Sbjct: 370 DCSHAGGSEAGFQRPNSCL 388

[Close]
BLAST of Lsi05G008440 vs. TAIR10
Match: AT2G43870.1 (AT2G43870.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 174.5 bits (441), Expect = 4.7e-44
Identity = 89/139 (64.03%), Postives = 106/139 (76.26%), Query Frame = 1 

Query: 3   ENGVQNVTMKSATFTNTQNGVRIKTWGRPSKGFAKNILFQDIVMVNVENPIIIDQNYCPD 62
           E+GVQNVT+K+ TFT T NGVRIK+W RPS GFAKNI FQ  VM NVENPIIIDQNYCPD
Sbjct: 248 ESGVQNVTVKTVTFTGTDNGVRIKSWARPSSGFAKNIRFQHCVMNNVENPIIIDQNYCPD 307

Query: 63  NKGCLGQDYGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSNITLEDVKLNYKNEAAKA 122
           +  C  Q  G+KISDV + +IHGTSAT+V +K DCSSK PC+ I LEDVKL Y+N+ A +
Sbjct: 308 H-DCPRQVSGIKISDVLFVDIHGTSATEVGVKLDCSSKKPCTGIRLEDVKLTYQNKPAAS 367

Query: 123 SYSHAEGSAARLVQPSSCL 142
           + +HA G  A   QP +CL
Sbjct: 368 ACTHAGGIEAGFFQP-NCL 384

[Close]
BLAST of Lsi05G008440 vs. TAIR10
Match: AT1G05660.1 (AT1G05660.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 172.2 bits (435), Expect = 2.3e-43
Identity = 81/142 (57.04%), Postives = 105/142 (73.94%), Query Frame = 1 

Query: 1   MQENGVQNVTMKSATFTNTQNGVRIKTWGRPSKGFAKNILFQDIVMVNVENPIIIDQNYC 60
           ++E+GV+NVT+ S+ FT +QNGVRIK+W RPS GF + + FQD+VM NVENPIIIDQNYC
Sbjct: 251 LKEDGVENVTVSSSVFTGSQNGVRIKSWARPSNGFVRTVFFQDLVMKNVENPIIIDQNYC 310

Query: 61  PDNKGCLGQDYGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSNITLEDVKLNYKNEAA 120
           P ++GC  +  GVKIS VTY+NI GTSATQ AMK  CS   PC+ ITL+D+KL Y     
Sbjct: 311 PTHEGCPNEYSGVKISQVTYKNIQGTSATQEAMKLVCSKSSPCTGITLQDIKLTYNKGTP 370

Query: 121 KASYS-HAEGSAARLVQPSSCL 142
             S+  +A G +  ++QP+SCL
Sbjct: 371 ATSFCFNAVGKSLGVIQPTSCL 392

[Close]
BLAST of Lsi05G008440 vs. TAIR10
Match: AT1G05650.1 (AT1G05650.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 169.1 bits (427), Expect = 2.0e-42
Identity = 80/142 (56.34%), Postives = 105/142 (73.94%), Query Frame = 1 

Query: 1   MQENGVQNVTMKSATFTNTQNGVRIKTWGRPSKGFAKNILFQDIVMVNVENPIIIDQNYC 60
           + E+GV+NVT+ S+ FT +QNGVRIK+W RPS GF +N+ FQ+++M NV+NPIIIDQNYC
Sbjct: 251 LNEDGVENVTVSSSVFTGSQNGVRIKSWARPSTGFVRNVFFQNLIMRNVQNPIIIDQNYC 310

Query: 61  PDNKGCLGQDYGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSNITLEDVKLNY-KNEA 120
           P N+GC  +  GVKI+ VTY+NI GTSATQ AMK  CS   PC+ ITL+D+KL Y K   
Sbjct: 311 PSNQGCPTEHSGVKITQVTYKNIQGTSATQEAMKLVCSKSNPCTGITLQDIKLTYNKGTP 370

Query: 121 AKASYSHAEGSAARLVQPSSCL 142
           A +   +A G    ++QP+SCL
Sbjct: 371 ATSLCFNAVGKNLGVIQPTSCL 392

[Close]
BLAST of Lsi05G008440 vs. TAIR10
Match: AT2G43880.1 (AT2G43880.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 166.0 bits (419), Expect = 1.7e-41
Identity = 75/139 (53.96%), Postives = 101/139 (72.66%), Query Frame = 1 

Query: 3   ENGVQNVTMKSATFTNTQNGVRIKTWGRPSKGFAKNILFQDIVMVNVENPIIIDQNYCPD 62
           E GVQNVT+ S+ FT TQNGVRIKTW RPS+GF  N++F++++M NVENP+IIDQNYCP+
Sbjct: 256 EEGVQNVTVTSSVFTKTQNGVRIKTWARPSRGFVNNVVFRNLIMNNVENPVIIDQNYCPN 315

Query: 63  NKGCLGQDYGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSNITLEDVKLNYKNEAAKA 122
            KGC  Q  GVKIS VT+ NI GTS T +AMK DCS    C+ + L+D+KL Y   ++ +
Sbjct: 316 GKGCPRQSSGVKISGVTFANIKGTSTTPIAMKLDCSGSNHCTGLRLQDIKLTYMRRSSAS 375

Query: 123 SYSHAEGSAARLVQPSSCL 142
              +A G A+ ++ P +C+
Sbjct: 376 YCRNAHGRASGVMVPRNCM 394

[Close]
BLAST of Lsi05G008440 vs. NCBI nr
Match: gi|659099184|ref|XP_008450474.1| (PREDICTED: polygalacturonase-like [Cucumis melo])

HSP 1 Score: 255.8 bits (652), Expect = 4.5e-65
Identity = 122/141 (86.52%), Postives = 136/141 (96.45%), Query Frame = 1 

Query: 1   MQENGVQNVTMKSATFTNTQNGVRIKTWGRPSKGFAKNILFQDIVMVNVENPIIIDQNYC 60
           +QE+GV+NVT+KSATFTNTQNGVRIKTWG+PS GFA++++FQDIVMVNVENPIIIDQNYC
Sbjct: 165 VQEDGVENVTVKSATFTNTQNGVRIKTWGKPSNGFARSVIFQDIVMVNVENPIIIDQNYC 224

Query: 61  PDNKGCLGQDYGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSNITLEDVKLNYKNEAA 120
           PDNKGC GQD GVKISDVTYRNIHGTSATQVAMKFDCSSKFPC++ITLEDV+L+YKNEAA
Sbjct: 225 PDNKGCPGQDSGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCNDITLEDVELSYKNEAA 284

Query: 121 KASYSHAEGSAARLVQPSSCL 142
           +AS SHAEGSAA LVQPSSCL
Sbjct: 285 EASCSHAEGSAAGLVQPSSCL 305

[Close]
BLAST of Lsi05G008440 vs. NCBI nr
Match: gi|449442581|ref|XP_004139060.1| (PREDICTED: polygalacturonase-like [Cucumis sativus])

HSP 1 Score: 255.0 bits (650), Expect = 7.7e-65
Identity = 123/141 (87.23%), Postives = 135/141 (95.74%), Query Frame = 1 

Query: 1   MQENGVQNVTMKSATFTNTQNGVRIKTWGRPSKGFAKNILFQDIVMVNVENPIIIDQNYC 60
           +QE+GV+NVT+KSATFTNTQNGVRIKTWG+PS GFA++++FQDIVMVNVENPIIIDQNYC
Sbjct: 246 VQEDGVENVTVKSATFTNTQNGVRIKTWGKPSNGFARSVIFQDIVMVNVENPIIIDQNYC 305

Query: 61  PDNKGCLGQDYGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSNITLEDVKLNYKNEAA 120
           PDNKGC GQD GVKISDVTYRNIHGTSATQVAMKFDCSSKFPCS+ITLEDVKL+ KNEAA
Sbjct: 306 PDNKGCPGQDSGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSDITLEDVKLSCKNEAA 365

Query: 121 KASYSHAEGSAARLVQPSSCL 142
           +AS SHAEGSAA LVQPSSCL
Sbjct: 366 EASCSHAEGSAAGLVQPSSCL 386

[Close]
BLAST of Lsi05G008440 vs. NCBI nr
Match: gi|645242107|ref|XP_008227395.1| (PREDICTED: polygalacturonase-like [Prunus mume])

HSP 1 Score: 222.2 bits (565), Expect = 5.6e-55
Identity = 105/140 (75.00%), Postives = 124/140 (88.57%), Query Frame = 1 

Query: 2   QENGVQNVTMKSATFTNTQNGVRIKTWGRPSKGFAKNILFQDIVMVNVENPIIIDQNYCP 61
           +E GVQNVT+KS TF NTQNG+RIK+WGRPS GFAK+ILFQ  VM+NV+NPI+IDQNYCP
Sbjct: 253 EEAGVQNVTVKSITFFNTQNGLRIKSWGRPSTGFAKDILFQHAVMLNVQNPIVIDQNYCP 312

Query: 62  DNKGCLGQDYGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSNITLEDVKLNYKNEAAK 121
           DNKGC GQ  GVKISDVTY++IHGTSAT+VA+KFDCSSK+PCSNI +EDVKL YKN+AA+
Sbjct: 313 DNKGCPGQVSGVKISDVTYQDIHGTSATEVAVKFDCSSKYPCSNIKMEDVKLTYKNQAAE 372

Query: 122 ASYSHAEGSAARLVQPSSCL 142
           AS SHA G++   VQP+SCL
Sbjct: 373 ASCSHAAGTSQGTVQPTSCL 392

[Close]
BLAST of Lsi05G008440 vs. NCBI nr
Match: gi|470132690|ref|XP_004302209.1| (PREDICTED: polygalacturonase-like [Fragaria vesca subsp. vesca])

HSP 1 Score: 222.2 bits (565), Expect = 5.6e-55
Identity = 105/140 (75.00%), Postives = 125/140 (89.29%), Query Frame = 1 

Query: 2   QENGVQNVTMKSATFTNTQNGVRIKTWGRPSKGFAKNILFQDIVMVNVENPIIIDQNYCP 61
           QE+GVQNVT+++ATFT TQNGVRIK+WGRPS GFAKNILFQ  VM+NV+NPI+IDQNYCP
Sbjct: 253 QEDGVQNVTVRTATFTGTQNGVRIKSWGRPSTGFAKNILFQHAVMINVQNPIVIDQNYCP 312

Query: 62  DNKGCLGQDYGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSNITLEDVKLNYKNEAAK 121
             KGC GQ  GV+ISDVTY++IHGTSAT+VA+KFDCSSK+PCS ITL+DVKL+YKN+AA+
Sbjct: 313 AEKGCPGQVSGVQISDVTYQDIHGTSATEVAVKFDCSSKYPCSKITLDDVKLSYKNQAAE 372

Query: 122 ASYSHAEGSAARLVQPSSCL 142
           AS SHA G+A   VQP+SCL
Sbjct: 373 ASCSHAGGTAEGTVQPTSCL 392

[Close]
BLAST of Lsi05G008440 vs. NCBI nr
Match: gi|593789550|ref|XP_007157814.1| (hypothetical protein PHAVU_002G100400g [Phaseolus vulgaris])

HSP 1 Score: 221.9 bits (564), Expect = 7.3e-55
Identity = 103/139 (74.10%), Postives = 120/139 (86.33%), Query Frame = 1 

Query: 2   QENGVQNVTMKSATFTNTQNGVRIKTWGRPSKGFAKNILFQDIVMVNVENPIIIDQNYCP 61
           +E GVQNVT+K+ TFT TQNGVRIKTWGRPS GF +N+LFQD +MVNVENP+IIDQNYCP
Sbjct: 258 REAGVQNVTVKTVTFTGTQNGVRIKTWGRPSDGFVRNVLFQDAIMVNVENPVIIDQNYCP 317

Query: 62  DNKGCLGQDYGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSNITLEDVKLNYKNEAAK 121
           DNKGC GQ  GVK+SDVTY++IHGTSATQVA+KFDCSSK+PCS I LEDVKL YKN+ A 
Sbjct: 318 DNKGCPGQASGVKVSDVTYQDIHGTSATQVAVKFDCSSKYPCSRIRLEDVKLTYKNQRAL 377

Query: 122 ASYSHAEGSAARLVQPSSC 141
           +S +HA+G+A   VQP SC
Sbjct: 378 SSCNHADGAALGSVQPESC 396

The following BLAST results are available for this feature:
BLAST of Lsi05G008440 vs. Swiss-Prot
Match NameE-valueIdentityDescription
PGLR_PRUPE6.1e-5470.71Polygalacturonase OS=Prunus persica PE=2 SV=1[more]
PGLR6_ARATH3.0e-3251.43Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana GN=At2g43860 PE=2 S... [more]
PGLR_ACTDE2.2e-2749.26Polygalacturonase OS=Actinidia deliciosa PE=2 SV=1[more]
QRT2_ARATH9.2e-2646.27Polygalacturonase QRT2 OS=Arabidopsis thaliana GN=QRT2 PE=1 SV=2[more]
PGLR2_ARATH7.8e-2545.00Exopolygalacturonase clone GBGA483 OS=Arabidopsis thaliana GN=At3g07850 PE=2 SV=... [more]
BLAST of Lsi05G008440 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0M095_CUCSA5.4e-6587.23Uncharacterized protein OS=Cucumis sativus GN=Csa_1G627460 PE=3 SV=1[more]
V7CKC9_PHAVU5.1e-5574.10Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_002G100400g PE=3 SV=1[more]
W9RN73_9ROSA8.6e-5573.05Uncharacterized protein OS=Morus notabilis GN=L484_020358 PE=3 SV=1[more]
M5WQ98_PRUPE1.5e-5474.29Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa021953mg PE=3 SV=1[more]
A0A151RM69_CAJCA1.9e-5472.34Polygalacturonase OS=Cajanus cajan GN=KK1_034877 PE=3 SV=1[more]
BLAST of Lsi05G008440 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G59850.15.9e-4762.59 Pectin lyase-like superfamily protein[more]
AT2G43870.14.7e-4464.03 Pectin lyase-like superfamily protein[more]
AT1G05660.12.3e-4357.04 Pectin lyase-like superfamily protein[more]
AT1G05650.12.0e-4256.34 Pectin lyase-like superfamily protein[more]
AT2G43880.11.7e-4153.96 Pectin lyase-like superfamily protein[more]
BLAST of Lsi05G008440 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659099184|ref|XP_008450474.1|4.5e-6586.52PREDICTED: polygalacturonase-like [Cucumis melo][more]
gi|449442581|ref|XP_004139060.1|7.7e-6587.23PREDICTED: polygalacturonase-like [Cucumis sativus][more]
gi|645242107|ref|XP_008227395.1|5.6e-5575.00PREDICTED: polygalacturonase-like [Prunus mume][more]
gi|470132690|ref|XP_004302209.1|5.6e-5575.00PREDICTED: polygalacturonase-like [Fragaria vesca subsp. vesca][more]
gi|593789550|ref|XP_007157814.1|7.3e-5574.10hypothetical protein PHAVU_002G100400g [Phaseolus vulgaris][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0005975carbohydrate metabolic process
Vocabulary: Molecular Function
TermDefinition
GO:0004650polygalacturonase activity
Vocabulary: INTERPRO
TermDefinition
IPR012334Pectin_lyas_fold
IPR011050Pectin_lyase_fold/virulence
IPR000743Glyco_hydro_28
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071555 cell wall organization
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0004650 polygalacturonase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G008440.1Lsi05G008440.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 3..125
score: 1.5
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 2..141
score: 1.64
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 2..140
score: 1.3
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 1..141
score: 2.7
NoneNo IPR availablePANTHERPTHR31375:SF2POLYGALACTURONASE/PECTINASEcoord: 1..141
score: 2.7

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Lsi05G008440ClCG09G014760Watermelon (Charleston Gray)lsiwcgB314
Lsi05G008440Cla97C09G176990Watermelon (97103) v2lsiwmbB352
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Lsi05G008440Cucurbita moschata (Rifu)cmolsiB476
Lsi05G008440Wax gourdlsiwgoB461