Lsi05G008380 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi05G008380
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Polygalacturonase, putative
Location	chr05 : 13694166 .. 13696836 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGTTCTAGGAACCTTACATCTACTTCTTTCCCTTCTGCTGCTGTTTTTCAACCATAACTTGGTAGCTGGACTAACATTCAATATCGTTAGTCTCGGAGCCATGCCCGACGGCAAGACCGACGCTTCACCTGCATTTCAAATGGCATGGGCCAATGCCTGTGGGTCGTCGGAACCAGCCACCATTTACGTGCCCGACGGCGTATTCTACCTTCAAAGTGGGAGCTTTAAAGGGCCTTGTAAGAACAATGCTATTACAATTCTCATCAAGGGAACTCTTATGGCTTCCTCAGACATTCAGGTTTTAGCTAAAACTGAATCTTGGATAGCCTTTAAACACATAAATGGGCTTTCCATTTCTGGGGGTGTTCTTGATGGGCAAGGAATTGGATTATGGAATTGTAAGCACTCGGGAAAGAGTTGCCCCGATGGAGCCACGGTATGCAAAATTTTAATACAAAAATACATGAAGGCAATTGAAATATTTTATTTTTAATTTGTACTAATTCTTGCTTTGATTGTTTTGAATGTGTGTGTAGAATCTTGAAATCTTCAATGCACAGAACGTTAAGGTCAGTGGATTATCTTCGGTTAATAGTCAAATGTTCAACATTGTGGTGGATGGTTGCCAAAATGTGCAAATCCAAGGAGTGAATGTCTCGTCTGCCGGCGATAGTCCGAATACGGATGGCATCCATGTCCAGCAATCATTGAACGTAAACATTCAGGACTCGAGCATCGGTACTGGTGATGACTGCATCTCAATTGGTCCAGGGACTACCAACTTGTGGATGGAAAATATTACATGTGGACCTGGACATGGAATTAGGTAAGTAAGGATAAGTGACAATAATGAACAACATATTTGCTAGGGTTGAAACCACCTTTGTCACTACTCAAAATCAAAATTGATAAAATGAAAATCTGAGTTTAAAAAATTACACTTTTTTTTTCTAAGCAAAATTTTAAAAACTATTTTTTTGAAGGACTCAAAATTTGACTAGGTTTTTGAAAACATATTTAAATAGTAGGTAATAAAGGAAGAAAATTAGCATCATCATGAATAGTGATAGTATAAACTTTTTTTTTAATAAACAAAATGGCTATCACATACGGGACATAAATAATTGATTTTAAATAATTATAGTTGTGTTTGGGAGTAATTTTGAAAGGATTAAAATTATTTTTGTCATAGTTAAAATCACTAAGAATTAAATTGATTTTCAGTAATTAACAATAGTTATTTTAATCATTTCCAAATCATTCTCAAACATGTCCTAAAGACATGGTGTAAAGTGATATTAACTCTTTCAAACATAGTTGAAAACATGTCCAAAGTAGTTACATAATATATTCAAGATATAACGATGAAAGAAATGAATGAAAATTCTGTGAAAATTTTTTATCCTCCATTCATTACTTGTCATAATAGTAATATTTAAGAATAATGTAGGATCAATAAAATAAATTAATATATATCATTGAATGATGTGTTAAATTATAAATGAGAGTTATTGGGTAGGACAGTAGTAGAATTTTTTCCGAAAATTGGAGTGTTTGTTGAATAATAATGTGCGTGTTGTTATAATTGTAGCATTGGGAGCTTGGGGAAGGAGGCAGAAGAGGCTGGAGTGGAAAACGTGACGGTGACAACAGCTACTTTCACAGGGACTGAGAATGGAGTGAGGATTAAATCTTGGGGAAGGCCAAGCGATGGCTTCGCTAAAAAAGTTCTTTTTCAACACATTCTTTTTAATAATGTCCGCAACCCTCTACTCATTGATCAAAATTACTGCCCAAATCACGATCCCTGCTCCGGTCAGGTACGTTATTTTCTTGAGACATTGAGTTGAATTTTGAAGTCTAAAGTTAATACATTAGAGTTAGGAAGTTTGTGTGTTGGGGTGATGAGTTGTTTAGTCTAGAGTTGGAAACATTAGACGGAGGGAGAGAGAAAGAAGGGAAGATGAAATTCTTGAATAAATGTACAAACTTTAATAGTGAATACTATTGAAGTAGGAAGTAGAATTGAGTTATTTAACCTCAACTTATACAATCGGTGCGAGCTAAACACTCCCTAAATGTTATGCATACACCTTAAATTTCCAATTTCACAAGTAATTAATTTACTTTGATGGAGAATGAATTGCATTCGACGATGAAACCCTAATAAATATCTACAATTGATTGATAATTGTCTATTTTGGTCATGATCACAACTTTGTTTTTAGTTTCACTCTAAAGCCTTCATATCATTGGAGATGGTTGTGCCCTCACTTATATATTATAATATTCTTTTTGTTTAGCTAAACATGAAACTTTGATCGCATTCCTAGATTGTTGCAATGATTAGTTTGATTCTCGAAATAAAAAAAGTATAGCATTGTGAATGACTCAGTAAATCTAGTTTTCATAGCATTTTGATTTTTCTTCTTTGCAATGACTAGGCTTCTGGAGTGAAAATTAGCGATGTTACATACCAAGACATGCATGGAACATCCGCCACTGAAGTAGCAATCAACTTTAACTGTAGCCCAACAAATCCTTGCACTGGATTAAGGTTGGAAGACATAAAGCTGACTTACAACAATCAGATTCCCCAAGCTTCATGTAAAAATGCTCAAGGAACTTCCTCTGGTCTTGTTCAACCTTCTAGTTGCTTAGCATAG

mRNA sequence

ATGGCTGTTCTAGGAACCTTACATCTACTTCTTTCCCTTCTGCTGCTGTTTTTCAACCATAACTTGGTAGCTGGACTAACATTCAATATCGTTAGTCTCGGAGCCATGCCCGACGGCAAGACCGACGCTTCACCTGCATTTCAAATGGCATGGGCCAATGCCTGTGGGTCGTCGGAACCAGCCACCATTTACGTGCCCGACGGCGTATTCTACCTTCAAAGTGGGAGCTTTAAAGGGCCTTGTAAGAACAATGCTATTACAATTCTCATCAAGGGAACTCTTATGGCTTCCTCAGACATTCAGGTTTTAGCTAAAACTGAATCTTGGATAGCCTTTAAACACATAAATGGGCTTTCCATTTCTGGGGGTGTTCTTGATGGGCAAGGAATTGGATTATGGAATTGTAAGCACTCGGGAAAGAGTTGCCCCGATGGAGCCACGAATCTTGAAATCTTCAATGCACAGAACGTTAAGGTCAGTGGATTATCTTCGGTTAATAGTCAAATGTTCAACATTGTGGTGGATGGTTGCCAAAATGTGCAAATCCAAGGAGTGAATGTCTCGTCTGCCGGCGATAGTCCGAATACGGATGGCATCCATGTCCAGCAATCATTGAACGTAAACATTCAGGACTCGAGCATCGGTACTGGTGATGACTGCATCTCAATTGGTCCAGGGACTACCAACTTGTGGATGGAAAATATTACATGTGGACCTGGACATGGAATTAGCATTGGGAGCTTGGGGAAGGAGGCAGAAGAGGCTGGAGTGGAAAACGTGACGGTGACAACAGCTACTTTCACAGGGACTGAGAATGGAGCTTCTGGAGTGAAAATTAGCGATGTTACATACCAAGACATGCATGGAACATCCGCCACTGAAGTAGCAATCAACTTTAACTGTAGCCCAACAAATCCTTGCACTGGATTAAGGTTGGAAGACATAAAGCTGACTTACAACAATCAGATTCCCCAAGCTTCATGTAAAAATGCTCAAGGAACTTCCTCTGGTCTTGTTCAACCTTCTAGTTGCTTAGCATAG

Coding sequence (CDS)

ATGGCTGTTCTAGGAACCTTACATCTACTTCTTTCCCTTCTGCTGCTGTTTTTCAACCATAACTTGGTAGCTGGACTAACATTCAATATCGTTAGTCTCGGAGCCATGCCCGACGGCAAGACCGACGCTTCACCTGCATTTCAAATGGCATGGGCCAATGCCTGTGGGTCGTCGGAACCAGCCACCATTTACGTGCCCGACGGCGTATTCTACCTTCAAAGTGGGAGCTTTAAAGGGCCTTGTAAGAACAATGCTATTACAATTCTCATCAAGGGAACTCTTATGGCTTCCTCAGACATTCAGGTTTTAGCTAAAACTGAATCTTGGATAGCCTTTAAACACATAAATGGGCTTTCCATTTCTGGGGGTGTTCTTGATGGGCAAGGAATTGGATTATGGAATTGTAAGCACTCGGGAAAGAGTTGCCCCGATGGAGCCACGAATCTTGAAATCTTCAATGCACAGAACGTTAAGGTCAGTGGATTATCTTCGGTTAATAGTCAAATGTTCAACATTGTGGTGGATGGTTGCCAAAATGTGCAAATCCAAGGAGTGAATGTCTCGTCTGCCGGCGATAGTCCGAATACGGATGGCATCCATGTCCAGCAATCATTGAACGTAAACATTCAGGACTCGAGCATCGGTACTGGTGATGACTGCATCTCAATTGGTCCAGGGACTACCAACTTGTGGATGGAAAATATTACATGTGGACCTGGACATGGAATTAGCATTGGGAGCTTGGGGAAGGAGGCAGAAGAGGCTGGAGTGGAAAACGTGACGGTGACAACAGCTACTTTCACAGGGACTGAGAATGGAGCTTCTGGAGTGAAAATTAGCGATGTTACATACCAAGACATGCATGGAACATCCGCCACTGAAGTAGCAATCAACTTTAACTGTAGCCCAACAAATCCTTGCACTGGATTAAGGTTGGAAGACATAAAGCTGACTTACAACAATCAGATTCCCCAAGCTTCATGTAAAAATGCTCAAGGAACTTCCTCTGGTCTTGTTCAACCTTCTAGTTGCTTAGCATAG

Protein sequence

MAVLGTLHLLLSLLLLFFNHNLVAGLTFNIVSLGAMPDGKTDASPAFQMAWANACGSSEPATIYVPDGVFYLQSGSFKGPCKNNAITILIKGTLMASSDIQVLAKTESWIAFKHINGLSISGGVLDGQGIGLWNCKHSGKSCPDGATNLEIFNAQNVKVSGLSSVNSQMFNIVVDGCQNVQIQGVNVSSAGDSPNTDGIHVQQSLNVNIQDSSIGTGDDCISIGPGTTNLWMENITCGPGHGISIGSLGKEAEEAGVENVTVTTATFTGTENGASGVKISDVTYQDMHGTSATEVAINFNCSPTNPCTGLRLEDIKLTYNNQIPQASCKNAQGTSSGLVQPSSCLA

Homology

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BLAST of Lsi05G008380 vs. Swiss-Prot
Match: PGLR_PRUPE (Polygalacturonase OS=Prunus persica PE=2 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 8.7e-94
Identity = 189/386 (48.96%), Postives = 245/386 (63.47%), Query Frame = 1


Query: 11  LSLLLLFFNHNLVAG-LTFNIVSLGAMPDGKTDASPAFQMAWANACGSSEPATIYVPDGV 70
           LSL+ +F  ++ +A  LT+N+ SLGA  DGKTD++ AF  AWA AC S  P  IYVP G 
Sbjct: 10  LSLIFVFMINSAIASPLTYNVASLGAKADGKTDSTKAFLSAWAKACASMNPGVIYVPAGT 69

Query: 71  FYLQSGSFKGPCKNNAITILIKGTLMASSDIQVLAKTESWIAFKHINGLSISGGVLDGQG 130
           F+L+   F GPCKNNAIT  I GTL+A SD +V+    +WI F H+NG++ISGG+LDGQG
Sbjct: 70  FFLRDVVFSGPCKNNAITFRIAGTLVAPSDYRVIGNAANWIFFHHVNGVTISGGILDGQG 129

Query: 131 IGLWNCKHS-GKSCPDGATNLEIFNAQNVKVSGLSSVNSQMFNIVVDGCQNVQIQGVNVS 190
             LW CK   G+SCP GAT L   ++ N+ VSGL+S+NSQMF+IV++  QNVQ+QGV VS
Sbjct: 130 TALWACKACHGESCPSGATTLGFSDSNNIVVSGLASLNSQMFHIVINDFQNVQMQGVRVS 189

Query: 191 SAGDSPNTDGIHVQQSLNVNIQDSSIGTGDDCISIGPGTTNLWMENITCGPGHGISIGSL 250
            +G+SPNTDGIHVQ S  V I +S I TGDDC+SIGPGT+NLW+E + CGPGHGISIGSL
Sbjct: 190 RSGNSPNTDGIHVQMSSGVTILNSKIATGDDCVSIGPGTSNLWIEGVACGPGHGISIGSL 249

Query: 251 GKEAEEAGVENVTVTTATFTGTENG--------ASGVKISDVTYQDMHGTSA---TEVAI 310
           GKE EEAGV+NVTV T TF+GT+NG         S     ++ +Q  H T       + I
Sbjct: 250 GKEQEEAGVQNVTVKTVTFSGTQNGLRIKSWGRPSTGFARNILFQ--HATMVNVENPIVI 309

Query: 311 NFNCSPTN-----PCTGLRLEDI---------------------------------KLTY 346
           + +  P N       +G+++ D+                                 KLTY
Sbjct: 310 DQHYCPDNKGCPGQVSGVQISDVTYEDIHGTSATEVAVKFDCSPKHPCREIKLEDVKLTY 369

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BLAST of Lsi05G008380 vs. Swiss-Prot
Match: PGLR6_ARATH (Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana GN=At2g43860 PE=2 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 5.0e-81
Identity = 169/397 (42.57%), Postives = 236/397 (59.45%), Query Frame = 1


Query: 3   VLGTLHLLLSLLLLFFNHNLVAGL------TFNIVSLGAMPDGKTDASPAFQMAWANACG 62
           V  T  L+L L L+  +  L++ L      T N++S GA PDG  D++ AF  AW  AC 
Sbjct: 2   VYNTSCLILPLALIILDFTLISSLAHPIPSTLNVLSYGAKPDGSKDSTKAFLAAWDVACA 61

Query: 63  SSEPATIYVPDGVFYLQSGSFKG-PCKNNAITILIKGTLMASSDIQVLAKTESWIAFKHI 122
           S+ P TI VP G F + +  F G  CK   I+I I G+++A  D +++A ++ WI F+ +
Sbjct: 62  SANPTTIIVPKGRFLVGNLVFHGNECKQAPISIRIAGSIVAPEDFRIIASSKHWIWFEDV 121

Query: 123 NGLSISGGVLDGQGIGLWNCKHSG-KSCPDGATNLEIFNAQNVKVSGLSSVNSQMFNIVV 182
             +SI GG+LD QG  LW CK++G  +CP GA +L    + N+K+SGL+S+NSQ F+IV+
Sbjct: 122 TDVSIYGGILDAQGTSLWKCKNNGGHNCPTGAKSLVFSGSNNIKISGLTSINSQKFHIVI 181

Query: 183 DGCQNVQIQGVNVSSAGDSPNTDGIHVQQSLNVNIQDSSIGTGDDCISIGPGTTNLWMEN 242
           D   NV I GV VS+  +SPNTDGIHV+ S +V+I +S IGTGDDCISIGPG+TN++++ 
Sbjct: 182 DNSNNVNIDGVKVSADENSPNTDGIHVESSHSVHITNSRIGTGDDCISIGPGSTNVFIQT 241

Query: 243 ITCGPGHGISIGSLGKEAEEAGVENVTVTTATFTG------------------------- 302
           I CGPGHGISIGSLG+  EE GV+NVTV+   F G                         
Sbjct: 242 IRCGPGHGISIGSLGRAEEEQGVDNVTVSNVDFMGTNNGVRIKTWGKDSNSFARNIVFQH 301

Query: 303 ---------------------TENGASGVKISDVTYQDMHGTSATEVAINFNCSPTNPCT 346
                                     SGVK+S+V Y+D+HGTS TEVA+  +CS   PCT
Sbjct: 302 INMKMVKNPIIIDQHYCLHKPCPKQESGVKVSNVRYEDIHGTSNTEVAVLLDCSKEKPCT 361

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BLAST of Lsi05G008380 vs. Swiss-Prot
Match: PGLR2_MAIZE (Exopolygalacturonase OS=Zea mays GN=PG9 PE=2 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 7.0e-51
Identity = 122/325 (37.54%), Postives = 185/325 (56.92%), Query Frame = 1


Query: 25  GLTFNIVSLGAMPDGKTDASPAFQMAWANACGSSEPATIYVPDGVFYLQSGSFKGPCKNN 84
           G +F+I  LGA  +GKTD++ A Q AWA+ACG +   TI +P G F +   +F GPCK +
Sbjct: 38  GGSFDITKLGASGNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFLVGQLNFTGPCKGD 97

Query: 85  AITILIKGTLMASSDIQVLAKTESWIAFKHINGLSISG-GVLDGQGIGLWNCKHSGKS-- 144
            +TI + G L+A++D+    +  +WI    ++ L I+G G LDGQG  +W+     K   
Sbjct: 98  -VTIQVDGNLLATTDLSQYKEHGNWIEILRVDNLVITGKGNLDGQGPAVWSKNSCTKKYD 157

Query: 145 CPDGATNLEIFNAQNVKVSGLSSVNSQMFNIVVDGCQNVQIQGVNVSSAGDSPNTDGIHV 204
           C     +L +    N +VSG++ +NS+ F++ +  C+N+ I+ V V++ GDSPNTDGIH+
Sbjct: 158 CKILPNSLVMDFVNNGEVSGVTLLNSKFFHMNMYQCKNMLIKDVTVTAPGDSPNTDGIHM 217

Query: 205 QQSLNVNIQDSSIGTGDDCISIGPGTTNLWMENITCGPGHGISIGSLGKEAEEAGVENVT 264
             S  + I ++ IG GDDCISIGPGT+ + +  +TCGPGHGISIGSLG+  +E  V ++ 
Sbjct: 218 GDSSGITITNTVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDVTDIN 277

Query: 265 VTTATFTGT---------ENGASGVKISDVTYQDMH-GTSATEVAINFNCSPTNPCTGLR 324
           V   T   T         E+ AS + +S + Y+++    SA  + I+    P   CT   
Sbjct: 278 VKDCTLKKTMFGVRIKAYEDAASVLTVSKIHYENIKMEDSANPIFIDMKYCPNKLCTANG 337

Query: 325 LEDIKLTYNNQIPQASCKNAQGTSS 337
              +       +   + KN  GTSS
Sbjct: 338 ASKV------TVKDVTFKNITGTSS 355

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BLAST of Lsi05G008380 vs. Swiss-Prot
Match: PGLR3_MAIZE (Exopolygalacturonase OS=Zea mays GN=PG2C PE=2 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 1.6e-50
Identity = 123/325 (37.85%), Postives = 183/325 (56.31%), Query Frame = 1


Query: 25  GLTFNIVSLGAMPDGKTDASPAFQMAWANACGSSEPATIYVPDGVFYLQSGSFKGPCKNN 84
           G +F+I  LGA  +GKTD++ A Q AWA+ACG +   TI +P G F +   +F GPCK +
Sbjct: 38  GGSFDITKLGASGNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFLVGPLNFTGPCKGD 97

Query: 85  AITILIKGTLMASSDIQVLAKTESWIAFKHINGLSISG-GVLDGQGIGLWNCKHSGKS-- 144
            +TI + G L+A++D+       +WI    ++ L I+G G LDGQG  +W+     K   
Sbjct: 98  -VTIQVNGNLLATTDLSQYKDHGNWIEILRVDNLVITGKGKLDGQGPAVWSKNSCVKKYD 157

Query: 145 CPDGATNLEIFNAQNVKVSGLSSVNSQMFNIVVDGCQNVQIQGVNVSSAGDSPNTDGIHV 204
           C     +L +    N +VSG++ +NS+ F++ +  C+++ I+ VNV++ GDSPNTDGIH+
Sbjct: 158 CKILPNSLVMDFVNNGEVSGITLLNSKFFHMNMYKCKDMLIKDVNVTAPGDSPNTDGIHM 217

Query: 205 QQSLNVNIQDSSIGTGDDCISIGPGTTNLWMENITCGPGHGISIGSLGKEAEEAGVENVT 264
             S  V I ++ IG GDDCISIGPGT+ + +  +TCGPGHGISIGSLG+  +E  V ++ 
Sbjct: 218 GDSSGVTITNTVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDVTDIN 277

Query: 265 VTTATFTGTENG---------ASGVKISDVTYQDMH-GTSATEVAINFNCSPTNPCTGLR 324
           V   T   T NG         AS +  S + Y+++    S   + I+    P   CT   
Sbjct: 278 VKDCTLKKTANGVRIKAYEDAASVLTASKIHYENIKMEDSGYPIIIDMKYCPNKLCTANG 337

Query: 325 LEDIKLTYNNQIPQASCKNAQGTSS 337
              +       +   + KN  GTSS
Sbjct: 338 ASKV------TVKDVTFKNITGTSS 355

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BLAST of Lsi05G008380 vs. Swiss-Prot
Match: ADPG2_ARATH (Polygalacturonase ADPG2 OS=Arabidopsis thaliana GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 200.7 bits (509), Expect = 2.7e-50
Identity = 121/289 (41.87%), Postives = 164/289 (56.75%), Query Frame = 1


Query: 17  FFNHNLVAGLTFNIVSLGAMPDGKTDASPAFQMAWANACGSSEPATIYVPDGVFYL-QSG 76
           F   +L    T ++   GA  DGKTD + AF  AW  AC S+    + VP G  YL +S 
Sbjct: 57  FVRASLRTPTTVSVSDFGAKGDGKTDDTQAFVNAWKKACSSNGAVNLLVPKGNTYLLKSI 116

Query: 77  SFKGPCKNNAITILIKGTLMASSDIQVLAKTESWIAFKHINGLSISGG---VLDGQGIGL 136
              GPC N+ +T+ I GTL AS           WI F  +N LS+ GG   V+DG G   
Sbjct: 117 QLTGPC-NSILTVQIFGTLSASQKRSDYKDISKWIMFDGVNNLSVDGGDTGVVDGNGETW 176

Query: 137 W--NCKHS-GKSCPDGATNLEIFNAQNVKVSGLSSVNSQMFNIVVDGCQNVQIQGVNVSS 196
           W  +CK +  K C    T L  +N++++ V  L   N+Q   I ++ C NVQ+  V V++
Sbjct: 177 WQNSCKRNKAKPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSNVQVSNVVVTA 236

Query: 197 AGDSPNTDGIHVQQSLNVNIQDSSIGTGDDCISIGPGTTNLWMENITCGPGHGISIGSLG 256
             DSPNTDGIH+  + N+ + +S IGTGDDCISI  G+ N+ + +ITCGPGHGISIGSLG
Sbjct: 237 PADSPNTDGIHITNTQNIRVSESIIGTGDDCISIESGSQNVQINDITCGPGHGISIGSLG 296

Query: 257 KEAEEAGVENVTVTTATFTGTENGASGVKISDVTYQDMHGTSATEVAIN 299
            +  +A V  VTV  A  +GT+N   GV+I   TYQ   GT++  +  N
Sbjct: 297 DDNSKAFVSGVTVDGAKLSGTDN---GVRIK--TYQGGSGTASNIIFQN 339

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BLAST of Lsi05G008380 vs. TrEMBL
Match: A0A0A0KN91_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G409610 PE=3 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 2.6e-145
Identity = 273/393 (69.47%), Postives = 306/393 (77.86%), Query Frame = 1


Query: 1   MAVLGTLHLLLSLLLLFFNHNLVAGLTFNIVSLGAMPDGKTDASPAFQMAWANACGSSEP 60
           M ++G L LL SLL LF NH L AG TFNIVSLGA+ DGKTDAS AFQ AWANACGSSEP
Sbjct: 1   MPLVGNLPLL-SLLFLFLNHGLAAGSTFNIVSLGAIADGKTDASHAFQEAWANACGSSEP 60

Query: 61  ATIYVPDGVFYLQSGSFKGPCKNNAITILIKGTLMASSDIQVLAKTESWIAFKHINGLSI 120
           ATIYVP+G FY+QSG+F GPCKNNAITI+I GTL+ASS+IQVLA++++WIAF+ INGLSI
Sbjct: 61  ATIYVPNGAFYIQSGNFSGPCKNNAITIVINGTLIASSNIQVLAQSQAWIAFRQINGLSI 120

Query: 121 SGGVLDGQGIGLWNCKHSGKSCPDGATNLEIFNAQNVKVSGLSSVNSQMFNIVVDGCQNV 180
            GGV+DGQGIGLWNCKHSGKSCPDGATNLEI +AQNV V+GLSS+NSQMFNIVV GC+NV
Sbjct: 121 YGGVIDGQGIGLWNCKHSGKSCPDGATNLEISHAQNVNVNGLSSINSQMFNIVVYGCENV 180

Query: 181 QIQGVNVSSAGDSPNTDGIHVQQSLNVNIQDSSIGTGDDCISIGPGTTNLWMENITCGPG 240
           QIQGVNVSSAGDSPNTDGIHVQQSLNVNI  +SIGTGDDCISIGPGTTNLWMENI CGPG
Sbjct: 181 QIQGVNVSSAGDSPNTDGIHVQQSLNVNISSTSIGTGDDCISIGPGTTNLWMENIKCGPG 240

Query: 241 HGISIGSLGKEAEEAGV-------------------------ENVTVTTATFT------- 300
           HGISIGSLG++ EEAGV                          N       F        
Sbjct: 241 HGISIGSLGRQVEEAGVENVTVTTATFTGTQNGVRIKSWGRPSNAFANKILFQHIRFYNV 300

Query: 301 ---------------GTENGASGVKISDVTYQDMHGTSATEVAINFNCSPTNPCTGLRLE 347
                          G    ASGVK+SDVTYQD++GTSA+EVAINF+CSPTNPCTG+ LE
Sbjct: 301 NNPILIDQNYCPNNQGCPGQASGVKVSDVTYQDVNGTSASEVAINFDCSPTNPCTGITLE 360

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BLAST of Lsi05G008380 vs. TrEMBL
Match: U5FZU4_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0010s25450g PE=3 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 1.8e-114
Identity = 203/364 (55.77%), Postives = 264/364 (72.53%), Query Frame = 1


Query: 9   LLLSLLLLFFNHNLVAGLTFNIVSLGAMPDGKTDASPAFQMAWANACGSSEPATIYVPDG 68
           +LL+LL++ F   L      N++S GA PDG TD++ AF  AWA AC S  P+TIYVP G
Sbjct: 10  VLLALLVMVFTSYLAIAEQHNVLSYGAKPDGGTDSTTAFLAAWAQACSSISPSTIYVPPG 69

Query: 69  VFYLQSGSFKGPCKNNAITILIKGTLMASSDIQVLAKTESWIAFKHINGLSISGGVLDGQ 128
            F+LQ+  FKGPCKN AI + I GTL+A +D +++    +WI F++++G+++SGGVLDG 
Sbjct: 70  RFFLQNVEFKGPCKNKAIVVRIDGTLLAPTDYRIIGNAGNWILFQNVDGVTVSGGVLDGH 129

Query: 129 GIGLWNCKHSGKSCPDGATNLEIFNAQNVKVSGLSSVNSQMFNIVVDGCQNVQIQGVNVS 188
           G GLW+CK SGK+CP GAT L   N+ N+ +SGL+S+NSQMF+IV++GC +V+IQGV ++
Sbjct: 130 GSGLWDCKRSGKNCPSGATTLRFSNSNNIVISGLTSLNSQMFHIVINGCNSVKIQGVRIT 189

Query: 189 SAGDSPNTDGIHVQQSLNVNIQDSSIGTGDDCISIGPGTTNLWMENITCGPGHGISIGSL 248
           ++G+SPNTDGIHVQ S N+ I  S IGTGDDC+SIG GTTNLW+EN+ CGPGHGISIGSL
Sbjct: 190 ASGNSPNTDGIHVQLSSNITILSSKIGTGDDCVSIGAGTTNLWIENVVCGPGHGISIGSL 249

Query: 249 GKEAEEAGVENVTVTTATFTGTENG---------------------------ASGVKISD 308
           GK+  E GV+NVT  T TF GT+NG                           ASGVKISD
Sbjct: 250 GKDLNEPGVQNVTAKTITFIGTQNGLRIKPSDGFVRNVLFQHAAMTNVQNPIASGVKISD 309

Query: 309 VTYQDMHGTSATEVAINFNCSPTNPCTGLRLEDIKLTYNNQIPQASCKNAQGTSSGLVQP 346
           V YQD+HGTSAT+VA+ F+CS   PCTG+++ED+KLTY NQ   ASC NA GT+SG++QP
Sbjct: 310 VIYQDIHGTSATQVAVRFDCSIKFPCTGIKMEDVKLTYKNQPAGASCNNADGTTSGVIQP 369

[Close]

BLAST of Lsi05G008380 vs. TrEMBL
Match: B9SSX1_RICCO (Polygalacturonase, putative OS=Ricinus communis GN=RCOM_1267820 PE=3 SV=1)

HSP 1 Score: 406.4 bits (1043), Expect = 3.6e-110
Identity = 208/382 (54.45%), Postives = 259/382 (67.80%), Query Frame = 1


Query: 11  LSLLLLFFNHNLVAGLTFNIVSLGAMPDGKTDASPAFQMAWANACGSSEPATIYVPDGVF 70
           L+LL+L F  +L     +N++S GA PDG+TD++ AF  AW  ACGS++P TIYVP G F
Sbjct: 12  LALLILLFTSSLATAAQYNVLSYGAKPDGRTDSTKAFLAAWTQACGSTKPPTIYVPSGKF 71

Query: 71  YLQSGSFKGPCKNNAITILIKGTLMASSDIQVLAKTESWIAFKHINGLSISGGVLDGQGI 130
           +L+  SF GPCKNNAI   I GTL+A SD +V+     W+ F+H+NG++ISGG+LDGQG 
Sbjct: 72  FLKDLSFGGPCKNNAILFRIDGTLVAPSDYKVIGNAGYWLYFQHVNGVTISGGILDGQGS 131

Query: 131 GLWNCKHSGKSCPDGATNLEIFNAQNVKVSGLSSVNSQMFNIVVDGCQNVQIQGVNVSSA 190
           GLW CK SGK+CP GAT+L   N++N+ ++GL+S NSQMF+IV++GCQNV++QGV VS++
Sbjct: 132 GLWACKASGKNCPSGATSLGFSNSKNIAINGLTSQNSQMFHIVINGCQNVKVQGVTVSAS 191

Query: 191 GDSPNTDGIHVQQSLNVNIQDSSIGTGDDCISIGPGTTNLWMENITCGPGHGISIGSLGK 250
           GDSPNTDGIHVQQS  V I +S I TGDDCISIGPG TNLW+ENI CGPGHGISIGSLGK
Sbjct: 192 GDSPNTDGIHVQQSGGVTILNSKIRTGDDCISIGPGATNLWIENIACGPGHGISIGSLGK 251

Query: 251 EAE-----------------EAGVE-------------NVTVTTATFTGTENGA------ 310
           + +                 + GV              N+    A  T  +N        
Sbjct: 252 DLQEAGVQNVTVKTVTFTGTQNGVRIKSWGRPSSGFARNILFQHAIMTNVQNPIVIDQNY 311

Query: 311 -----------SGVKISDVTYQDMHGTSATEVAINFNCSPTNPCTGLRLEDIKLTYNNQI 346
                      SGVKIS VTYQD+HGTSATEVA+ F+CS   PCTG++LED+KLTY NQ 
Sbjct: 312 CPDNKNCPGQESGVKISGVTYQDIHGTSATEVAVKFDCSKKEPCTGIKLEDVKLTYKNQP 371

[Close]

BLAST of Lsi05G008380 vs. TrEMBL
Match: S5MMR8_9ROSA (Polygalacturonase 2 OS=Pyrus x bretschneideri GN=PG2 PE=2 SV=1)

HSP 1 Score: 402.1 bits (1032), Expect = 6.7e-109
Identity = 204/368 (55.43%), Postives = 251/368 (68.21%), Query Frame = 1


Query: 24  AGLTFNIVSLGAMPDGKTDASPAFQMAWANACGSSEPATIYVPDGVFYLQSGSFKGPCKN 83
           A +TF++ SLGA  DG TD++ AF  AW+NAC S  PA IYVP G F L +  F GPCKN
Sbjct: 30  AAVTFSVSSLGAKADGSTDSTKAFLSAWSNACASVNPAVIYVPAGRFLLSNAVFSGPCKN 89

Query: 84  NAITILIKGTLMASSDIQVLAKTESWIAFKHINGLSISGGVLDGQGIGLWNCKHSGKSCP 143
           NAIT  I GTL+A SD QV+    +W+ F+H+NG++ISGGVLDGQG GLW+CK SGKSCP
Sbjct: 90  NAITFRIAGTLVAPSDYQVIGNAGNWLLFQHVNGVTISGGVLDGQGTGLWDCKSSGKSCP 149

Query: 144 DGATNLEIFNAQNVKVSGLSSVNSQMFNIVVDGCQNVQIQGVNVSSAGDSPNTDGIHVQQ 203
            GAT L   N+ NV VSGL S+NSQMF+IVV+GCQNV++QGV V++AG+SPNTDGIHVQ 
Sbjct: 150 SGATTLSFSNSNNVVVSGLVSLNSQMFHIVVNGCQNVKMQGVKVNAAGNSPNTDGIHVQM 209

Query: 204 SLNVNIQDSSIGTGDDCISIGPGTTNLWMENITCGPGHGISIGSLGKEAE---------- 263
           S  V I DS I TGDDC+S+GPGTTNLW+EN+ CGPGHGISIGSLGK+ +          
Sbjct: 210 SSGVTILDSKISTGDDCVSVGPGTTNLWIENVACGPGHGISIGSLGKDQQEAGVQNVTVK 269

Query: 264 -------EAGV--------------------------ENVTVTTATFTGTENG----ASG 323
                  E GV                          +N  +    +   + G    ASG
Sbjct: 270 TITFTGTENGVRIKSWGRPSTGFARSILFQHIVMTNVQNPIIIDQNYCPNDKGCPGQASG 329

Query: 324 VKISDVTYQDMHGTSATEVAINFNCSPTNPCTGLRLEDIKLTYNNQIPQASCKNAQGTSS 345
           VK+SDVTYQD+HGTSATEVA+ F+CS   PC G+RL+D+KLTYNNQ  +ASC +A GT++
Sbjct: 330 VKVSDVTYQDIHGTSATEVAVKFDCSSMYPCNGIRLQDVKLTYNNQAAEASCIHAGGTTA 389

[Close]

BLAST of Lsi05G008380 vs. TrEMBL
Match: G8FU52_PYRPY (Polygalacturonase OS=Pyrus pyrifolia GN=PG2 PE=2 SV=1)

HSP 1 Score: 401.7 bits (1031), Expect = 8.7e-109
Identity = 205/368 (55.71%), Postives = 251/368 (68.21%), Query Frame = 1


Query: 24  AGLTFNIVSLGAMPDGKTDASPAFQMAWANACGSSEPATIYVPDGVFYLQSGSFKGPCKN 83
           A +TF++ SLGA  DG TD++ AF  AW+NAC S  PA IYVP G F L +  F GPCKN
Sbjct: 30  AAVTFSVSSLGAKADGSTDSTKAFLSAWSNACASVNPAVIYVPAGRFLLGNAVFSGPCKN 89

Query: 84  NAITILIKGTLMASSDIQVLAKTESWIAFKHINGLSISGGVLDGQGIGLWNCKHSGKSCP 143
           NAIT  I GTL+A SD QV+    +W+ F+H+NG++ISGGVLDGQG GLW+CK SGKSCP
Sbjct: 90  NAITFRIAGTLVAPSDYQVIGNAGNWLLFQHVNGVTISGGVLDGQGTGLWDCKSSGKSCP 149

Query: 144 DGATNLEIFNAQNVKVSGLSSVNSQMFNIVVDGCQNVQIQGVNVSSAGDSPNTDGIHVQQ 203
            GAT L   N+ NV VSGL S+NSQMF+IVV+GCQNV++QGV V++AG+SPNTDGIHVQ 
Sbjct: 150 SGATTLSFSNSNNVVVSGLISLNSQMFHIVVNGCQNVKMQGVKVNAAGNSPNTDGIHVQM 209

Query: 204 SLNVNIQDSSIGTGDDCISIGPGTTNLWMENITCGPGHGISIGSLGKEAE---------- 263
           S  V I DS I TGDDC+S+GPGTTNLW+EN+ CGPGHGISIGSLGK+ +          
Sbjct: 210 SSGVTILDSKISTGDDCVSVGPGTTNLWIENVACGPGHGISIGSLGKDQQEAGVQNVTVK 269

Query: 264 -------EAGV--------------------------ENVTVTTATFTGTENG----ASG 323
                  E GV                          +N  V    +   + G    ASG
Sbjct: 270 TVTFTGTENGVRIKSWGRPSTGFARSILFQHIIMTNVQNPIVIDQNYCPNDKGCPGQASG 329

Query: 324 VKISDVTYQDMHGTSATEVAINFNCSPTNPCTGLRLEDIKLTYNNQIPQASCKNAQGTSS 345
           VK+SDVTYQD+HGTSATEVA+ F+CS   PC G+RL+D+KLTYNNQ  +ASC +A GT++
Sbjct: 330 VKVSDVTYQDIHGTSATEVAVKFDCSSMYPCNGIRLQDVKLTYNNQAAEASCIHAGGTTA 389

[Close]

BLAST of Lsi05G008380 vs. TAIR10
Match: AT2G43870.1 (AT2G43870.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 323.2 bits (827), Expect = 2.0e-88
Identity = 174/384 (45.31%), Postives = 238/384 (61.98%), Query Frame = 1


Query: 11  LSLLLLFFNHNLVAGLTFNIVSLGAMPDGKTDASPAFQMAWANACGSSEPATIYVPDGVF 70
           L +L +FF  +  +  ++N++S GA PDGKTDA+ AF   W  AC SS P TI VP G F
Sbjct: 4   LLVLFVFFFISSCSAQSYNVLSFGAKPDGKTDATKAFMAVWQTACASSRPVTIVVPKGRF 63

Query: 71  YLQSGSFKGP-CKNNAITILIKGTLMASSDIQVLAKTESWIAFKHINGLSISGGVLDGQG 130
            L+S +F G  CK   +T  I GTL+A +D +V+   + WI F+H++G+++ GGVLD +G
Sbjct: 64  LLRSVTFDGSKCKPKPVTFRIDGTLVAPADYRVIGNEDYWIFFQHLDGITVYGGVLDARG 123

Query: 131 IGLWNCKHSGKSCPDGATNLEIFNAQNVKVSGLSSVNSQMFNIVVDGCQNVQIQGVNVSS 190
             LW+CK SGK+CP GAT +   ++ NV VSGL+S+NSQMF++V++GC NV++QGV V +
Sbjct: 124 ASLWDCKKSGKNCPSGATTIGFQSSSNVVVSGLTSLNSQMFHVVINGCNNVKLQGVKVLA 183

Query: 191 AGDSPNTDGIHVQQSLNVNIQDSSIGTGDDCISIGPGTTNLWMENITCGPGHG--ISIGS 250
           AG+SPNTDGIHVQ S +V+I ++ I TGDDC+SIGPGT  LW+EN+ CGPGHG  I  GS
Sbjct: 184 AGNSPNTDGIHVQSSSSVSIFNTKISTGDDCVSIGPGTNGLWIENVACGPGHGISI--GS 243

Query: 251 LGKEA-----------------EEAGV-------------ENVTVTTATFTGTENG---- 310
           LGK++                  + GV             +N+          EN     
Sbjct: 244 LGKDSVESGVQNVTVKTVTFTGTDNGVRIKSWARPSSGFAKNIRFQHCVMNNVENPIIID 303

Query: 311 ------------ASGVKISDVTYQDMHGTSATEVAINFNCSPTNPCTGLRLEDIKLTYNN 346
                        SG+KISDV + D+HGTSATEV +  +CS   PCTG+RLED+KLTY N
Sbjct: 304 QNYCPDHDCPRQVSGIKISDVLFVDIHGTSATEVGVKLDCSSKKPCTGIRLEDVKLTYQN 363

[Close]

BLAST of Lsi05G008380 vs. TAIR10
Match: AT3G59850.1 (AT3G59850.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 322.4 bits (825), Expect = 3.4e-88
Identity = 175/384 (45.57%), Postives = 239/384 (62.24%), Query Frame = 1


Query: 10  LLSLLLLFFNHNLVAGLTFNIVSLGAMPDGKTDASPAFQMAWANACGSSEPATIYVPDGV 69
           LL +LL   + +  +  T+NI+S GA PDGKTD++ AF + WA AC S +P TI VP G 
Sbjct: 5   LLIVLLFLLSVSSSSAQTYNILSYGAKPDGKTDSTKAFTVLWAKACASVKPVTILVPKGR 64

Query: 70  FYLQSGSFKGP-CKNNAITILIKGTLMASSDIQVLAKTESWIAFKHINGLSISGGVLDGQ 129
           F L+S  F G  CK  ++T  I+GTL+A SD +V+ K   WI F+H++G+S+ GGVLD Q
Sbjct: 65  FLLRSIIFDGSKCKRKSVTFRIQGTLVAPSDYRVIGKENYWILFQHLDGISVYGGVLDAQ 124

Query: 130 GIGLWNCKHSGKSCPDGATNLEIFNAQNVKVSGLSSVNSQMFNIVVDGCQNVQIQGVNVS 189
           G  LW+CK SGK+CP GAT++   +++NV +SGL+S+NSQMF++ ++GC NV++ GV VS
Sbjct: 125 GASLWSCKKSGKNCPSGATSIGFQSSRNVVISGLTSLNSQMFHVAINGCSNVKLDGVKVS 184

Query: 190 SAGDSPNTDGIHVQQSLNVNIQDSSIGTGDDCISIGPGTTNLWMENITCGPGHGISIGSL 249
           + G+SPNTDGIHVQ S  V+I +S I TGDDC+SIGPGT  LW+EN+ CGPGHGISIGSL
Sbjct: 185 ADGNSPNTDGIHVQSSSTVSILNSKISTGDDCVSIGPGTNGLWIENVACGPGHGISIGSL 244

Query: 250 GKEAEEAGVENVTVTTATFTGTENGA--------SGVKISDVTYQD-MHGTSATEVAINF 309
           GKE+ E GV+N+TV TATFTGTENG         S     ++ +Q  +       + I+ 
Sbjct: 245 GKESVEVGVQNITVKTATFTGTENGVRIKSWARPSNGFAKNIRFQHCVMNNVQNPIVIDQ 304

Query: 310 NCSPTNP-C------------------------TGLRLE-------------DIKLTYNN 346
           N  P N  C                         G++L+             D+KLTY N
Sbjct: 305 NYCPGNENCPNQVSGIKISDVMFFDIHGTSATEVGVKLDCSSKKPCTGIRIQDVKLTYRN 364

[Close]

BLAST of Lsi05G008380 vs. TAIR10
Match: AT2G43860.1 (AT2G43860.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 302.8 bits (774), Expect = 2.8e-82
Identity = 169/397 (42.57%), Postives = 236/397 (59.45%), Query Frame = 1


Query: 3   VLGTLHLLLSLLLLFFNHNLVAGL------TFNIVSLGAMPDGKTDASPAFQMAWANACG 62
           V  T  L+L L L+  +  L++ L      T N++S GA PDG  D++ AF  AW  AC 
Sbjct: 2   VYNTSCLILPLALIILDFTLISSLAHPIPSTLNVLSYGAKPDGSKDSTKAFLAAWDVACA 61

Query: 63  SSEPATIYVPDGVFYLQSGSFKG-PCKNNAITILIKGTLMASSDIQVLAKTESWIAFKHI 122
           S+ P TI VP G F + +  F G  CK   I+I I G+++A  D +++A ++ WI F+ +
Sbjct: 62  SANPTTIIVPKGRFLVGNLVFHGNECKQAPISIRIAGSIVAPEDFRIIASSKHWIWFEDV 121

Query: 123 NGLSISGGVLDGQGIGLWNCKHSG-KSCPDGATNLEIFNAQNVKVSGLSSVNSQMFNIVV 182
             +SI GG+LD QG  LW CK++G  +CP GA +L    + N+K+SGL+S+NSQ F+IV+
Sbjct: 122 TDVSIYGGILDAQGTSLWKCKNNGGHNCPTGAKSLVFSGSNNIKISGLTSINSQKFHIVI 181

Query: 183 DGCQNVQIQGVNVSSAGDSPNTDGIHVQQSLNVNIQDSSIGTGDDCISIGPGTTNLWMEN 242
           D   NV I GV VS+  +SPNTDGIHV+ S +V+I +S IGTGDDCISIGPG+TN++++ 
Sbjct: 182 DNSNNVNIDGVKVSADENSPNTDGIHVESSHSVHITNSRIGTGDDCISIGPGSTNVFIQT 241

Query: 243 ITCGPGHGISIGSLGKEAEEAGVENVTVTTATFTG------------------------- 302
           I CGPGHGISIGSLG+  EE GV+NVTV+   F G                         
Sbjct: 242 IRCGPGHGISIGSLGRAEEEQGVDNVTVSNVDFMGTNNGVRIKTWGKDSNSFARNIVFQH 301

Query: 303 ---------------------TENGASGVKISDVTYQDMHGTSATEVAINFNCSPTNPCT 346
                                     SGVK+S+V Y+D+HGTS TEVA+  +CS   PCT
Sbjct: 302 INMKMVKNPIIIDQHYCLHKPCPKQESGVKVSNVRYEDIHGTSNTEVAVLLDCSKEKPCT 361

[Close]

BLAST of Lsi05G008380 vs. TAIR10
Match: AT1G65570.1 (AT1G65570.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 289.7 bits (740), Expect = 2.5e-78
Identity = 156/372 (41.94%), Postives = 218/372 (58.60%), Query Frame = 1


Query: 24  AGLTFNIVSLGAMPDGKTDASPAFQMAWANACGSSEPATIYVPDGVFYLQSGS--FKGP- 83
           A  + N++S GA P+G  +++ AF  AW  ACG  +   IYVP G  YL SG   F+G  
Sbjct: 27  ARTSLNVLSFGANPNGIVESAKAFSDAWDAACGVEDSVVIYVPKGR-YLVSGEVRFEGES 86

Query: 84  CKNNAITILIKGTLMASSDIQVLAKTESWIAFKHINGLSISGGVLDGQGIGLWNCKHSGK 143
           CK+  IT+ I GTL+   D  +L K E+W +F  ++ +++ GG  D +G  LW+CK +G 
Sbjct: 87  CKSREITLRIDGTLIGPQDYSLLGKKENWFSFSGVHNVTVLGGSFDAKGSTLWSCKANGY 146

Query: 144 SCPDGATNLEIFNAQNVKVSGLSSVNSQMFNIVVDGCQNVQIQGVNVSSAGDSPNTDGIH 203
           +CP+GAT L   ++ NVK+ G+ S+NSQ+F+I ++ C+N++I+ V + +  +SPNTDGIH
Sbjct: 147 NCPEGATTLRFMDSNNVKIKGVLSLNSQLFHIAINRCRNIKIEDVRIIAPDESPNTDGIH 206

Query: 204 VQQSLNVNIQDSSIGTGDDCISIGPGTTNLWMENITCGPGHGISIGSLGKEAEEAGVENV 263
           +Q S ++ ++++SI TGDDCISIGPGT NL ++ ITCGPGHGISIGSL K  EE GVENV
Sbjct: 207 IQLSTDIEVRNASIKTGDDCISIGPGTKNLMVDGITCGPGHGISIGSLAKSIEEQGVENV 266

Query: 264 TVT------------TATFTGTENG----------------------------------- 323
           TV               ++    NG                                   
Sbjct: 267 TVKNAVFVRTDNGLRIKSWPRHSNGFVERVRFLGAIMVNVSYPILIDQNYCPGDSSCPSQ 326

Query: 324 ASGVKISDVTYQDMHGTSATEVAINFNCSPTNPCTGLRLEDIKLTYNNQIPQASCKNAQG 346
            SG+KI+DV Y  + GTSATE+AI  +CS   PCTG+R++ I LT   +  + SC N  G
Sbjct: 327 ESGIKINDVIYSGIMGTSATEIAIKMDCSEKVPCTGIRMQAINLTSYGEAAKTSCTNVSG 386

[Close]

BLAST of Lsi05G008380 vs. TAIR10
Match: AT2G43890.1 (AT2G43890.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 266.9 bits (681), Expect = 1.7e-71
Identity = 152/388 (39.18%), Postives = 225/388 (57.99%), Query Frame = 1


Query: 10  LLSLLLLFFNHNLV-----AGLTFNIVSLGAMPDGKTDASPAFQMAWANACGSSEPATIY 69
           L+++LL+FF+  L+     A   +N+VS GA PDG+TD++ AF  AW  AC S+   T+ 
Sbjct: 6   LVAVLLMFFSSFLLMKSSTAASNYNVVSFGAKPDGRTDSTKAFLGAWQAACRSAAAVTVT 65

Query: 70  VPDGVFYLQSGSFKGPCKNNAITILIKGTLMASSDIQVLAKTESWIAFKHINGLSISGGV 129
           VP G F L+   F+GPC++  IT  I GT++A SD + L  +  WI F  +N +SI GG 
Sbjct: 66  VPRGSFLLKPVEFRGPCRSR-ITFQIYGTIVAPSDYRGLGNSGYWILFVKVNRISIIGGT 125

Query: 130 LDGQGIGLWNCKHSGKSCPDGATNLEIFNAQNVKVSGLSSVNSQMFNIVVDGCQNVQIQG 189
           LD +G   W C+ SGKSCP GA ++    A +V VSGL+S+NSQ  ++V++ C NV ++ 
Sbjct: 126 LDARGASFWACRKSGKSCPVGARSMTFNWANDVVVSGLTSINSQTTHLVINSCNNVIVRK 185

Query: 190 VNVSSAGDSPNTDGIHVQQSLNVNIQDSSIGTGDDCISIGPGTTNLWMENITCGPGHGIS 249
           V + +   SPNTDG+HVQ S  V + D +  TGDDCISIGPGT NL+M  + CGPGHGIS
Sbjct: 186 VKLVAPDQSPNTDGLHVQGSAGVTVTDGTFHTGDDCISIGPGTRNLYMSKLNCGPGHGIS 245

Query: 250 IGSLGKEAEEAGVENVTVTTATFTGTENGA--------SGVKISDVTYQDMHGTSA-TEV 309
           IGSLG++A EAGVEN+T+  + F+G++NG         S   + +V +Q++   +    +
Sbjct: 246 IGSLGRDANEAGVENITLINSVFSGSDNGVRIKTWARQSTGFVRNVLFQNLIMKNVQNPI 305

Query: 310 AINFNCSPTNP-C----TGLRLEDI---------------------------------KL 346
            ++ N  P+N  C    +G+++  +                                 KL
Sbjct: 306 IVDQNYCPSNQGCPKQGSGVKISQVVYRNIQGTSRTQQALTFDCSRSNPCQAIRLHDIKL 365

[Close]

BLAST of Lsi05G008380 vs. NCBI nr
Match: gi|449445220|ref|XP_004140371.1| (PREDICTED: polygalacturonase [Cucumis sativus])

HSP 1 Score: 523.1 bits (1346), Expect = 3.7e-145
Identity = 273/393 (69.47%), Postives = 306/393 (77.86%), Query Frame = 1


Query: 1   MAVLGTLHLLLSLLLLFFNHNLVAGLTFNIVSLGAMPDGKTDASPAFQMAWANACGSSEP 60
           M ++G L LL SLL LF NH L AG TFNIVSLGA+ DGKTDAS AFQ AWANACGSSEP
Sbjct: 1   MPLVGNLPLL-SLLFLFLNHGLAAGSTFNIVSLGAIADGKTDASHAFQEAWANACGSSEP 60

Query: 61  ATIYVPDGVFYLQSGSFKGPCKNNAITILIKGTLMASSDIQVLAKTESWIAFKHINGLSI 120
           ATIYVP+G FY+QSG+F GPCKNNAITI+I GTL+ASS+IQVLA++++WIAF+ INGLSI
Sbjct: 61  ATIYVPNGAFYIQSGNFSGPCKNNAITIVINGTLIASSNIQVLAQSQAWIAFRQINGLSI 120

Query: 121 SGGVLDGQGIGLWNCKHSGKSCPDGATNLEIFNAQNVKVSGLSSVNSQMFNIVVDGCQNV 180
            GGV+DGQGIGLWNCKHSGKSCPDGATNLEI +AQNV V+GLSS+NSQMFNIVV GC+NV
Sbjct: 121 YGGVIDGQGIGLWNCKHSGKSCPDGATNLEISHAQNVNVNGLSSINSQMFNIVVYGCENV 180

Query: 181 QIQGVNVSSAGDSPNTDGIHVQQSLNVNIQDSSIGTGDDCISIGPGTTNLWMENITCGPG 240
           QIQGVNVSSAGDSPNTDGIHVQQSLNVNI  +SIGTGDDCISIGPGTTNLWMENI CGPG
Sbjct: 181 QIQGVNVSSAGDSPNTDGIHVQQSLNVNISSTSIGTGDDCISIGPGTTNLWMENIKCGPG 240

Query: 241 HGISIGSLGKEAEEAGV-------------------------ENVTVTTATFT------- 300
           HGISIGSLG++ EEAGV                          N       F        
Sbjct: 241 HGISIGSLGRQVEEAGVENVTVTTATFTGTQNGVRIKSWGRPSNAFANKILFQHIRFYNV 300

Query: 301 ---------------GTENGASGVKISDVTYQDMHGTSATEVAINFNCSPTNPCTGLRLE 347
                          G    ASGVK+SDVTYQD++GTSA+EVAINF+CSPTNPCTG+ LE
Sbjct: 301 NNPILIDQNYCPNNQGCPGQASGVKVSDVTYQDVNGTSASEVAINFDCSPTNPCTGITLE 360

[Close]

BLAST of Lsi05G008380 vs. NCBI nr
Match: gi|659121214|ref|XP_008460550.1| (PREDICTED: polygalacturonase-like [Cucumis melo])

HSP 1 Score: 481.9 bits (1239), Expect = 9.5e-133
Identity = 258/393 (65.65%), Postives = 297/393 (75.57%), Query Frame = 1


Query: 1   MAVLGTLHLLLSLLLLFFNHNLVAGLTFNIVSLGAMPDGKTDASPAFQMAWANACGSSEP 60
           M ++G LHLL  LL LF NH L AG TFNIVSLGA  DG TDASPAFQ AWANACGSSEP
Sbjct: 1   MPLVGNLHLLF-LLFLFLNHGLAAGSTFNIVSLGAKADGMTDASPAFQEAWANACGSSEP 60

Query: 61  ATIYVPDGVFYLQSGSFKGPCKNNAITILIKGTLMASSDIQVLAKTESWIAFKHINGLSI 120
           ATIYVP+G FY+ SG+F GPCKNNAITILI GTLMASS+IQVLA++++WIAF+HINGLSI
Sbjct: 61  ATIYVPNGAFYVHSGNFTGPCKNNAITILINGTLMASSNIQVLAQSQAWIAFRHINGLSI 120

Query: 121 SGGVLDGQGIGLWNCKHSGKSCPDGATNLEIFNAQNVKVSGLSSVNSQMFNIVVDGCQNV 180
            GGV+DGQG+GLWNCKHSG+SCPDGAT+LEIF++QNV V+GLSSVNSQMFNIVV GCQNV
Sbjct: 121 YGGVIDGQGMGLWNCKHSGQSCPDGATSLEIFDSQNVNVNGLSSVNSQMFNIVVYGCQNV 180

Query: 181 QIQGVNVSSAGDSPNTDGIHVQQSLNVNIQDSSIGTGDDCISIGPGTTNLWM-------- 240
           QIQGVNVSSA DSPNTDGIHVQQSLNVNI +S+IGTGDDCISIGPGTTNLWM        
Sbjct: 181 QIQGVNVSSASDSPNTDGIHVQQSLNVNISNSNIGTGDDCISIGPGTTNLWMENINCGPG 240

Query: 241 -----------------ENITC------GPGHGISIGSLGKEAEEAGVENV-------TV 300
                            +N+T       G  +G+ I S G+ ++    + +        V
Sbjct: 241 HGISIGSLGKQAEEAGVQNVTVTTATFRGTQNGVRIKSWGRPSDGFATKILFQHILFYNV 300

Query: 301 TTATFTGTE---------NGASGVKISDVTYQDMHGTSATEVAINFNCSPTNPCTGLRLE 347
           +   F               ASGVKISDVTYQD++GTSATEVAINF+CSP+NPCTG+ LE
Sbjct: 301 SNPIFIDQNYCPHQQSCPGQASGVKISDVTYQDINGTSATEVAINFDCSPSNPCTGIHLE 360

[Close]

BLAST of Lsi05G008380 vs. NCBI nr
Match: gi|566192722|ref|XP_006378843.1| (hypothetical protein POPTR_0010s25450g [Populus trichocarpa])

HSP 1 Score: 420.6 bits (1080), Expect = 2.6e-114
Identity = 203/364 (55.77%), Postives = 264/364 (72.53%), Query Frame = 1


Query: 9   LLLSLLLLFFNHNLVAGLTFNIVSLGAMPDGKTDASPAFQMAWANACGSSEPATIYVPDG 68
           +LL+LL++ F   L      N++S GA PDG TD++ AF  AWA AC S  P+TIYVP G
Sbjct: 10  VLLALLVMVFTSYLAIAEQHNVLSYGAKPDGGTDSTTAFLAAWAQACSSISPSTIYVPPG 69

Query: 69  VFYLQSGSFKGPCKNNAITILIKGTLMASSDIQVLAKTESWIAFKHINGLSISGGVLDGQ 128
            F+LQ+  FKGPCKN AI + I GTL+A +D +++    +WI F++++G+++SGGVLDG 
Sbjct: 70  RFFLQNVEFKGPCKNKAIVVRIDGTLLAPTDYRIIGNAGNWILFQNVDGVTVSGGVLDGH 129

Query: 129 GIGLWNCKHSGKSCPDGATNLEIFNAQNVKVSGLSSVNSQMFNIVVDGCQNVQIQGVNVS 188
           G GLW+CK SGK+CP GAT L   N+ N+ +SGL+S+NSQMF+IV++GC +V+IQGV ++
Sbjct: 130 GSGLWDCKRSGKNCPSGATTLRFSNSNNIVISGLTSLNSQMFHIVINGCNSVKIQGVRIT 189

Query: 189 SAGDSPNTDGIHVQQSLNVNIQDSSIGTGDDCISIGPGTTNLWMENITCGPGHGISIGSL 248
           ++G+SPNTDGIHVQ S N+ I  S IGTGDDC+SIG GTTNLW+EN+ CGPGHGISIGSL
Sbjct: 190 ASGNSPNTDGIHVQLSSNITILSSKIGTGDDCVSIGAGTTNLWIENVVCGPGHGISIGSL 249

Query: 249 GKEAEEAGVENVTVTTATFTGTENG---------------------------ASGVKISD 308
           GK+  E GV+NVT  T TF GT+NG                           ASGVKISD
Sbjct: 250 GKDLNEPGVQNVTAKTITFIGTQNGLRIKPSDGFVRNVLFQHAAMTNVQNPIASGVKISD 309

Query: 309 VTYQDMHGTSATEVAINFNCSPTNPCTGLRLEDIKLTYNNQIPQASCKNAQGTSSGLVQP 346
           V YQD+HGTSAT+VA+ F+CS   PCTG+++ED+KLTY NQ   ASC NA GT+SG++QP
Sbjct: 310 VIYQDIHGTSATQVAVRFDCSIKFPCTGIKMEDVKLTYKNQPAGASCNNADGTTSGVIQP 369

[Close]

BLAST of Lsi05G008380 vs. NCBI nr
Match: gi|568880496|ref|XP_006493154.1| (PREDICTED: polygalacturonase-like [Citrus sinensis])

HSP 1 Score: 408.3 bits (1048), Expect = 1.3e-110
Identity = 210/388 (54.12%), Postives = 269/388 (69.33%), Query Frame = 1


Query: 6   TLHLLLSLLLLFFNHNLVAGLTFNIVSLGAMPDGKTDASPAFQMAWANACGSSEPATIYV 65
           +++ L  LL+LFF+ +L   L++N++S GA PDG+TD+  +F  AWA ACGS+E ATIYV
Sbjct: 8   SINFLPFLLILFFSSSLAIPLSYNVLSFGARPDGQTDSVKSFLAAWAQACGSAEAATIYV 67

Query: 66  PDGVFYLQSGSFKGPCKNNAITILIKGTLMASSDIQVLAKTESWIAFKHINGLSISGGVL 125
           P G + L +  F+G CKNN ITI I GTL+A +D  V+   E+WI F+H++G+SI+GGVL
Sbjct: 68  PPGRYLLSNVVFQGQCKNNDITIRIDGTLVAPADYHVIGNAENWILFEHVSGVSINGGVL 127

Query: 126 DGQGIGLWNCKHSGKS-CPDGATNLEIFNAQNVKVSGLSSVNSQMFNIVVDGCQNVQIQG 185
           DGQG GLW CK S  S CP GAT L   N+ NV ++GL+SV+SQMF+IV++GC +V++QG
Sbjct: 128 DGQGAGLWACKKSDNSNCPSGATTLRFSNSDNVVINGLASVDSQMFHIVINGCNDVKVQG 187

Query: 186 VNVSSAGDSPNTDGIHVQQSLNVNIQDSSIGTGDDCISIGPGTTNLWMENITCGPGHGIS 245
           V VS+AGDSPNTDGIHVQ S  V I DS IGTGDDC+S+GPG TNLW+EN+ CGPGHGIS
Sbjct: 188 VKVSAAGDSPNTDGIHVQASSGVTILDSKIGTGDDCVSVGPGATNLWIENVACGPGHGIS 247

Query: 246 IGSLGKEAEEAGVENVTVTT------------ATFTGTENG------------------- 305
           IGSLGKE +EAGV+NVTVT+             ++  T +G                   
Sbjct: 248 IGSLGKEQQEAGVQNVTVTSVTFTGTQNGVRIKSWGRTSSGFARNILFQHALMNNVDNPI 307

Query: 306 ----------------ASGVKISDVTYQDMHGTSATEVAINFNCSPTNPCTGLRLEDIKL 346
                           ASGVKISDV YQD+HGTSATEV +  +CS  NPCTG+ LED+KL
Sbjct: 308 IIDQNYCPDNENCPGQASGVKISDVIYQDIHGTSATEVGVKLDCSSRNPCTGISLEDVKL 367

[Close]

BLAST of Lsi05G008380 vs. NCBI nr
Match: gi|255576396|ref|XP_002529090.1| (PREDICTED: polygalacturonase [Ricinus communis])

HSP 1 Score: 406.4 bits (1043), Expect = 5.1e-110
Identity = 208/382 (54.45%), Postives = 259/382 (67.80%), Query Frame = 1


Query: 11  LSLLLLFFNHNLVAGLTFNIVSLGAMPDGKTDASPAFQMAWANACGSSEPATIYVPDGVF 70
           L+LL+L F  +L     +N++S GA PDG+TD++ AF  AW  ACGS++P TIYVP G F
Sbjct: 12  LALLILLFTSSLATAAQYNVLSYGAKPDGRTDSTKAFLAAWTQACGSTKPPTIYVPSGKF 71

Query: 71  YLQSGSFKGPCKNNAITILIKGTLMASSDIQVLAKTESWIAFKHINGLSISGGVLDGQGI 130
           +L+  SF GPCKNNAI   I GTL+A SD +V+     W+ F+H+NG++ISGG+LDGQG 
Sbjct: 72  FLKDLSFGGPCKNNAILFRIDGTLVAPSDYKVIGNAGYWLYFQHVNGVTISGGILDGQGS 131

Query: 131 GLWNCKHSGKSCPDGATNLEIFNAQNVKVSGLSSVNSQMFNIVVDGCQNVQIQGVNVSSA 190
           GLW CK SGK+CP GAT+L   N++N+ ++GL+S NSQMF+IV++GCQNV++QGV VS++
Sbjct: 132 GLWACKASGKNCPSGATSLGFSNSKNIAINGLTSQNSQMFHIVINGCQNVKVQGVTVSAS 191

Query: 191 GDSPNTDGIHVQQSLNVNIQDSSIGTGDDCISIGPGTTNLWMENITCGPGHGISIGSLGK 250
           GDSPNTDGIHVQQS  V I +S I TGDDCISIGPG TNLW+ENI CGPGHGISIGSLGK
Sbjct: 192 GDSPNTDGIHVQQSGGVTILNSKIRTGDDCISIGPGATNLWIENIACGPGHGISIGSLGK 251

Query: 251 EAE-----------------EAGVE-------------NVTVTTATFTGTENGA------ 310
           + +                 + GV              N+    A  T  +N        
Sbjct: 252 DLQEAGVQNVTVKTVTFTGTQNGVRIKSWGRPSSGFARNILFQHAIMTNVQNPIVIDQNY 311

Query: 311 -----------SGVKISDVTYQDMHGTSATEVAINFNCSPTNPCTGLRLEDIKLTYNNQI 346
                      SGVKIS VTYQD+HGTSATEVA+ F+CS   PCTG++LED+KLTY NQ 
Sbjct: 312 CPDNKNCPGQESGVKISGVTYQDIHGTSATEVAVKFDCSKKEPCTGIKLEDVKLTYKNQP 371

The following BLAST results are available for this feature:

BLAST of Lsi05G008380 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PGLR_PRUPE	8.7e-94	48.96	Polygalacturonase OS=Prunus persica PE=2 SV=1	[more]
PGLR6_ARATH	5.0e-81	42.57	Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana GN=At2g43860 PE=2 S...	[more]
PGLR2_MAIZE	7.0e-51	37.54	Exopolygalacturonase OS=Zea mays GN=PG9 PE=2 SV=1	[more]
PGLR3_MAIZE	1.6e-50	37.85	Exopolygalacturonase OS=Zea mays GN=PG2C PE=2 SV=1	[more]
ADPG2_ARATH	2.7e-50	41.87	Polygalacturonase ADPG2 OS=Arabidopsis thaliana GN=ADPG2 PE=2 SV=2	[more]

BLAST of Lsi05G008380 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KN91_CUCSA	2.6e-145	69.47	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G409610 PE=3 SV=1	[more]
U5FZU4_POPTR	1.8e-114	55.77	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0010s25450g PE=3 SV=1	[more]
B9SSX1_RICCO	3.6e-110	54.45	Polygalacturonase, putative OS=Ricinus communis GN=RCOM_1267820 PE=3 SV=1	[more]
S5MMR8_9ROSA	6.7e-109	55.43	Polygalacturonase 2 OS=Pyrus x bretschneideri GN=PG2 PE=2 SV=1	[more]
G8FU52_PYRPY	8.7e-109	55.71	Polygalacturonase OS=Pyrus pyrifolia GN=PG2 PE=2 SV=1	[more]

BLAST of Lsi05G008380 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G43870.1	2.0e-88	45.31	Pectin lyase-like superfamily protein	[more]
AT3G59850.1	3.4e-88	45.57	Pectin lyase-like superfamily protein	[more]
AT2G43860.1	2.8e-82	42.57	Pectin lyase-like superfamily protein	[more]
AT1G65570.1	2.5e-78	41.94	Pectin lyase-like superfamily protein	[more]
AT2G43890.1	1.7e-71	39.18	Pectin lyase-like superfamily protein	[more]

BLAST of Lsi05G008380 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449445220\|ref\|XP_004140371.1\|	3.7e-145	69.47	PREDICTED: polygalacturonase [Cucumis sativus]	[more]
gi\|659121214\|ref\|XP_008460550.1\|	9.5e-133	65.65	PREDICTED: polygalacturonase-like [Cucumis melo]	[more]
gi\|566192722\|ref\|XP_006378843.1\|	2.6e-114	55.77	hypothetical protein POPTR_0010s25450g [Populus trichocarpa]	[more]
gi\|568880496\|ref\|XP_006493154.1\|	1.3e-110	54.12	PREDICTED: polygalacturonase-like [Citrus sinensis]	[more]
gi\|255576396\|ref\|XP_002529090.1\|	5.1e-110	54.45	PREDICTED: polygalacturonase [Ricinus communis]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0005975	carbohydrate metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0004650	polygalacturonase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR012334	Pectin_lyas_fold
IPR011050	Pectin_lyase_fold/virulence
IPR006626	PbH1
IPR000743	Glyco_hydro_28

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004650	polygalacturonase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G008380.1	Lsi05G008380.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 55..273 score: 1.0E-53coord: 274..333 score: 5.4
IPR000743	Glycoside hydrolase, family 28	PROSITE	PS00502	POLYGALACTURONASE	coord: 234..247 scor
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 227..247 score: 6300.0coord: 204..225 score: 1800.0coord: 177..203 score: 9.4coord: 257..281 score: 14
IPR011050	Pectin lyase fold/virulence factor	unknown	SSF51126	Pectin lyase-like	coord: 11..345 score: 1.71
IPR012334	Pectin lyase fold	GENE3D	G3DSA:2.160.20.10		coord: 10..345 score: 3.8
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 6..345 score: 1.2E
None	No IPR available	PANTHER	PTHR31375:SF2	POLYGALACTURONASE/PECTINASE	coord: 6..345 score: 1.2E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi05G008380	Cla97C09G176970	Watermelon (97103) v2	lsiwmbB352

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi05G008380	Wax gourd	lsiwgoB461
Lsi05G008380	Watermelon (Charleston Gray)	lsiwcgB314