Lsi05G007570 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi05G007570
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Protein NRT1/ PTR FAMILY 4.3
Location	chr05 : 10535504 .. 10540804 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AGCCAACTAGAAGTTTGCCTCAGATTCAGCTGGAAGAAGAAGAGAGTAAGTAAGAACCAAAAAGAAAAGAAAAGAAAAGAAAACTCTCTTCAAGAAATCATTATTCTCATAAAACAAAGCTTTATTTGATTTCTATCTTGTAATGGAAATGGAGATGGAAAGGGAAAGAAAGAGAGATTTCAAGGTGGAGAGCCATACCAATATCCCATCTGAAGATGTCTCTGTTGATTGGAGAGGAAGACCCTCCAACCCGACCTCCCACGGCGGAATGAGAGCGGCTGCTTTTGTTCTCGGTAACTCTCTTTCTCTCGAGAAACTTTTTAGTTCTTTCGTAGTACCTATTTTCTTTATTAGTTTTTTTATATATATTTTTATATGCATACTCTATGTCATTTTTAAATATAATGTTGCTGACGTAGTTGAACATGAATGGAAGTAGTATGAAAAATTGAATTATTCTCATTTCTCTTATTTTCTAGTCCTATTTCGGATTTTTTTTTTCCTTTATTGGAAGTTTTAAAAAAATGGCTGTCTAATGGGTATGGCTTGAATCCTTTTAAGGGAGTATTAAAAAAAATTAAAAAGAAAAAAAAGAAAACTTTTTCTCTAAACAATGGCCTTGAAGTTAAAGATAAGTGGTTTTCTACCAGTTTGGTTCAAAGTCAATGGTTTTGGCCTTAATATATATATATATATATATATAAAATCAGTTTTGGTGAATATGATATGGAATTTGGCAAACCTAAGCCTAACCTCTTATCTAAATATTTTGTATCTCCTTTTAAAGGGTTATAGTTTCAAATCTTTTATCTGGCATTTCGAACTAAAACAATCGAGAAAAATAAATCTGATATAGAGTAACCGTGTCGTTGTATATGTTATCCGGTACGCAGGGCTGCAAGCGTTTGAGATAATGGCGATTGCTGCGGTTGGGAACAATTTGATAACATATTTGATGAATGAGATGCACTTTTCACTGTCAAAATCTGCAAACATAGTGACAAACTTTGTTGGGACAGTGTTTCTTTTGGCCCTTCTTGGTGGTTTCCTTTCTGATTCTTATCTTGGGAGCTTTTGGACCATTCTTATCTTTGGCTTTGTTGAACTTTCTGTAAGTTCTTCCCATCATTCATTCTTCCTTTCATTTCTATCAAAATCAGTCTTTTCAACACACTCTCTCTCTCTCTATATATATATATGACACATATCAAAGTGATCTCAAATTATATCCTTTCATGTTTATCAAAAACTTATTTAATTTATGTGCTTCTTTCTATCATAACATATAATCATTGTTCAATGAATCAATCACCTCCACTTTGGATAAAGATAGTTTATTTTTTAACCCACCAAGGAGAAGATTCAAATCATATATATATATTGGTTAAGAATGTAAATGAAATATTAATTAGGGGCTTAGGTTTTTAAGTTGATACGTATTATCAATGAAGCTCGTTAAATGTTTCTTTTTCTTTTGAAAAGTTGATACGTATTTTAACATTTATCGGAGAGGCTAAAGTTCTCATGTTCAAACTTCTATAATACAATTTTAACTTGTTAACCAGGTCTTTTCCTAATTTTCAAGCTCACAATTACCATGAAAGTAATAGATAACATCTAAGTTCACTAAAAATAACTTATTAGATATAATAACGTAAAAACTCATGCATATAATCTTTTTCTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN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mRNA sequence

AGCCAACTAGAAGTTTGCCTCAGATTCAGCTGGAAGAAGAAGAGACTTTATTTGATTTCTATCTTGTAATGGAAATGGAGATGGAAAGGGAAAGAAAGAGAGATTTCAAGGTGGAGAGCCATACCAATATCCCATCTGAAGATGTCTCTGTTGATTGGAGAGGAAGACCCTCCAACCCGACCTCCCACGGCGGAATGAGAGCGGCTGCTTTTGTTCTCGGGCTGCAAGCGTTTGAGATAATGGCGATTGCTGCGGTTGGGAACAATTTGATAACATATTTGATGAATGAGATGCACTTTTCACTGTCAAAATCTGCAAACATAGTGACAAACTTTGTTGGGACAGTGTTTCTTTTGGCCCTTCTTGGTGGTTTCCTTTCTGATTCTTATCTTGGGAGCTTTTGGACCATTCTTATCTTTGGCTTTGTTGAACTTTCTGATATTAAATTC

Coding sequence (CDS)

ATGGAAATGGAGATGGAAAGGGAAAGAAAGAGAGATTTCAAGGTGGAGAGCCATACCAATATCCCATCTGAAGATGTCTCTGTTGATTGGAGAGGAAGACCCTCCAACCCGACCTCCCACGGCGGAATGAGAGCGGCTGCTTTTGTTCTCGGGCTGCAAGCGTTTGAGATAATGGCGATTGCTGCGGTTGGGAACAATTTGATAACATATTTGATGAATGAGATGCACTTTTCACTGTCAAAATCTGCAAACATAGTGACAAACTTTGTTGGGACAGTGTTTCTTTTGGCCCTTCTTGGTGGTTTCCTTTCTGATTCTTATCTTGGGAGCTTTTGGACCATTCTTATCTTTGGCTTTGTTGAACTTTCTGATATTAAATTC

Protein sequence

MEMEMERERKRDFKVESHTNIPSEDVSVDWRGRPSNPTSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYLMNEMHFSLSKSANIVTNFVGTVFLLALLGGFLSDSYLGSFWTILIFGFVELSDIKF

Homology

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BLAST of Lsi05G007570 vs. Swiss-Prot
Match: PTR16_ARATH (Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana GN=NPF4.3 PE=2 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 6.3e-42
Identity = 83/101 (82.18%), Postives = 94/101 (93.07%), Query Frame = 1


Query: 23  SEDVSVDWRGRPSNPTSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYLMNEMHFSLSKS 82
           +E+ SVDWRGRPSNP  HGGMRAA FVLGLQAFEIM IAAVGNNLITY++NEMHF LSK+
Sbjct: 25  AEEESVDWRGRPSNPNKHGGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSKA 84

Query: 83  ANIVTNFVGTVFLLALLGGFLSDSYLGSFWTILIFGFVELS 124
           ANIVTNFVGT+F+ ALLGG+LSD++LGSFWTI+IFGFVELS
Sbjct: 85  ANIVTNFVGTIFIFALLGGYLSDAFLGSFWTIIIFGFVELS 125

[Close]

BLAST of Lsi05G007570 vs. Swiss-Prot
Match: PTR15_ARATH (Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana GN=NPF4.4 PE=2 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 4.7e-37
Identity = 79/107 (73.83%), Postives = 93/107 (86.92%), Query Frame = 1


Query: 18  HTNIPS-EDVSVDWRGRPSNPTSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYLMNEMH 77
           HT+  S +D+ VD+RGRP  P+ HGG RAA FVLG QAFE+MAIAAVGNNLITY+ NEMH
Sbjct: 16  HTSEESLDDLCVDFRGRPCRPSKHGGTRAALFVLGFQAFEMMAIAAVGNNLITYVFNEMH 75

Query: 78  FSLSKSANIVTNFVGTVFLLALLGGFLSDSYLGSFWTILIFGFVELS 124
           F LSKSAN+VTNF+GTVFLL+LLGGFLSDSYLGSF T+L+FG +E+S
Sbjct: 76  FPLSKSANLVTNFIGTVFLLSLLGGFLSDSYLGSFRTMLVFGVIEIS 122

[Close]

BLAST of Lsi05G007570 vs. Swiss-Prot
Match: PTR27_ARATH (Protein NRT1/ PTR FAMILY 6.2 OS=Arabidopsis thaliana GN=NPF6.2 PE=1 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 9.8e-19
Identity = 48/95 (50.53%), Postives = 67/95 (70.53%), Query Frame = 1


Query: 27  SVDWRGRPSNPTSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYLMNEMHFSLSKSANIV 86
           +VD++GRP++ +  GG   AA +LG++  E ++   +  NL+TYLM  MH   S SANIV
Sbjct: 12  AVDYKGRPADKSKTGGWITAALILGIEVVERLSTMGIAVNLVTYLMETMHLPSSTSANIV 71

Query: 87  TNFVGTVFLLALLGGFLSDSYLGSFWTILIFGFVE 122
           T+F+GT FLL LLGGFL+DS+LG F TI IF  ++
Sbjct: 72  TDFMGTSFLLCLLGGFLADSFLGRFKTIGIFSTIQ 106

[Close]

BLAST of Lsi05G007570 vs. Swiss-Prot
Match: PTR19_ARATH (Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana GN=NPF4.6 PE=1 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 1.1e-17
Identity = 50/93 (53.76%), Postives = 62/93 (66.67%), Query Frame = 1


Query: 29  DWRGRPSNPTSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYLMNEMHFSLSKSANIVTN 88
           DWR R +    HGGM AA+FVL ++  E +A  A  +NL+ YL   MH S SKSAN VTN
Sbjct: 15  DWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMSPSKSANDVTN 74

Query: 89  FVGTVFLLALLGGFLSDSYLGSFWTILIFGFVE 122
           F+GT FLLALLGGFLSD++  +F   LI   +E
Sbjct: 75  FMGTAFLLALLGGFLSDAFFSTFQIFLISASIE 107

[Close]

BLAST of Lsi05G007570 vs. Swiss-Prot
Match: PTR12_ARATH (Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana GN=NPF4.5 PE=2 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 5.4e-17
Identity = 47/108 (43.52%), Postives = 69/108 (63.89%), Query Frame = 1


Query: 14  KVESHTNIPSEDVSVDWRGRPSNPTSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYLMN 73
           +VE H ++   +   DWR + +    HGGM AA+FVL ++  E +A  A  +NL+ YL N
Sbjct: 2   EVEMHGDVSKWEGYADWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKN 61

Query: 74  EMHFSLSKSANIVTNFVGTVFLLALLGGFLSDSYLGSFWTILIFGFVE 122
            MH SL++S++ VT F+ T FLLALLGGFL+D++  +F   LI   +E
Sbjct: 62  FMHMSLARSSSEVTTFMATAFLLALLGGFLADAFFSTFVIFLISASIE 109

[Close]

BLAST of Lsi05G007570 vs. TrEMBL
Match: A0A0A0L2Q8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G112250 PE=4 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 1.7e-49
Identity = 104/121 (85.95%), Postives = 111/121 (91.74%), Query Frame = 1


Query: 3   MEMERERKRDFKVESHTNIPSEDVSVDWRGRPSNPTSHGGMRAAAFVLGLQAFEIMAIAA 62
           MEMER++ RD K E+H     +DVSVDWRGRPSNPTSHGGMRAA FVLG+Q FEIMAIAA
Sbjct: 1   MEMERQKMRDLKGENHPIPSDQDVSVDWRGRPSNPTSHGGMRAAVFVLGMQTFEIMAIAA 60

Query: 63  VGNNLITYLMNEMHFSLSKSANIVTNFVGTVFLLALLGGFLSDSYLGSFWTILIFGFVEL 122
           VGNNLITYL+NEMHFSLSKSANIVTNFVGTVFLLALLGGFLSDSYLGSFWT+LIFGFVEL
Sbjct: 61  VGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALLGGFLSDSYLGSFWTLLIFGFVEL 120

Query: 123 S 124
           S
Sbjct: 121 S 121

[Close]

BLAST of Lsi05G007570 vs. TrEMBL
Match: U5GKK1_POPTR (Proton-dependent oligopeptide transport family protein OS=Populus trichocarpa GN=POPTR_0004s23790g PE=4 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 1.1e-43
Identity = 97/121 (80.17%), Postives = 109/121 (90.08%), Query Frame = 1


Query: 3   MEMERERKRDFKVESHTNIPSEDVSVDWRGRPSNPTSHGGMRAAAFVLGLQAFEIMAIAA 62
           M+ME  R+ D K ES   + SE+V+VDWRG+PSNP  HGGMRAAAFVLGLQAFEIMAIAA
Sbjct: 1   MDMEA-RRHDTKGES-VCVVSEEVTVDWRGKPSNPNKHGGMRAAAFVLGLQAFEIMAIAA 60

Query: 63  VGNNLITYLMNEMHFSLSKSANIVTNFVGTVFLLALLGGFLSDSYLGSFWTILIFGFVEL 122
           VGNNLITY++NEMHFSLSKSANIVTNFVGT+F+LALLGG+LSDSYLG FWT+LIFGFVEL
Sbjct: 61  VGNNLITYVINEMHFSLSKSANIVTNFVGTIFILALLGGYLSDSYLGCFWTMLIFGFVEL 119

Query: 123 S 124
           S
Sbjct: 121 S 119

[Close]

BLAST of Lsi05G007570 vs. TrEMBL
Match: M5XC53_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa002962mg PE=4 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 3.1e-43
Identity = 95/124 (76.61%), Postives = 112/124 (90.32%), Query Frame = 1


Query: 3   MEMERERK-RDFKVESH--TNIPSEDVSVDWRGRPSNPTSHGGMRAAAFVLGLQAFEIMA 62
           MEME+ R+ ++ K E+   + +  ED+++DWRGRPSNPT HGGMRAAAFVLGLQ+FEIM 
Sbjct: 1   MEMEQRRQLQNHKGEAMITSEMGKEDITLDWRGRPSNPTKHGGMRAAAFVLGLQSFEIMG 60

Query: 63  IAAVGNNLITYLMNEMHFSLSKSANIVTNFVGTVFLLALLGGFLSDSYLGSFWTILIFGF 122
           IAAVGNNLITY++NEMHFSLSKSANIVTNF+GTVFLLAL+GG+LSDSYLGSFWT+LIFGF
Sbjct: 61  IAAVGNNLITYVINEMHFSLSKSANIVTNFIGTVFLLALVGGYLSDSYLGSFWTMLIFGF 120

Query: 123 VELS 124
           VELS
Sbjct: 121 VELS 124

[Close]

BLAST of Lsi05G007570 vs. TrEMBL
Match: A0A061G9D1_THECC (Major facilitator superfamily protein OS=Theobroma cacao GN=TCM_027118 PE=4 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 6.8e-43
Identity = 96/124 (77.42%), Postives = 105/124 (84.68%), Query Frame = 1


Query: 4   EMERERKRDFKVESHTN----IPSEDVSVDWRGRPSNPTSHGGMRAAAFVLGLQAFEIMA 63
           E ERE +     E   N    +  E+ +VDWRGRPSNP  HGGMRAAAFVLGLQAFEIMA
Sbjct: 32  EREREERGVQMEEGRRNNKVWVTEEESTVDWRGRPSNPNKHGGMRAAAFVLGLQAFEIMA 91

Query: 64  IAAVGNNLITYLMNEMHFSLSKSANIVTNFVGTVFLLALLGGFLSDSYLGSFWTILIFGF 123
           IAAVGNNLITY++NEMHFSLSKSANIVTNFVGT+FLLALLGG+LSDSYLGSFWT+LIFGF
Sbjct: 92  IAAVGNNLITYVINEMHFSLSKSANIVTNFVGTIFLLALLGGYLSDSYLGSFWTMLIFGF 151

[Close]

BLAST of Lsi05G007570 vs. TrEMBL
Match: B9SPB0_RICCO (Nitrate transporter, putative OS=Ricinus communis GN=RCOM_0496050 PE=4 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 4.4e-42
Identity = 92/123 (74.80%), Postives = 103/123 (83.74%), Query Frame = 1


Query: 1   MEMEMERERKRDFKVESHTNIPSEDVSVDWRGRPSNPTSHGGMRAAAFVLGLQAFEIMAI 60
           MEM   R   +  +++  + +  E  +VDWRGRPSNP  HGGMRAA FVLGLQAFEIMAI
Sbjct: 1   MEMSNARSNNKRGELKMSSVVSEESGTVDWRGRPSNPNKHGGMRAATFVLGLQAFEIMAI 60

Query: 61  AAVGNNLITYLMNEMHFSLSKSANIVTNFVGTVFLLALLGGFLSDSYLGSFWTILIFGFV 120
           AAVGNNLITY+MNEMHFSLS SANIVTNFVGTVF+LALLGG+LSDSYLG FWT+LIFGFV
Sbjct: 61  AAVGNNLITYVMNEMHFSLSSSANIVTNFVGTVFILALLGGYLSDSYLGCFWTMLIFGFV 120

Query: 121 ELS 124
           ELS
Sbjct: 121 ELS 123

[Close]

BLAST of Lsi05G007570 vs. TAIR10
Match: AT1G59740.1 (AT1G59740.1 Major facilitator superfamily protein)

HSP 1 Score: 171.4 bits (433), Expect = 3.6e-43
Identity = 83/101 (82.18%), Postives = 94/101 (93.07%), Query Frame = 1


Query: 23  SEDVSVDWRGRPSNPTSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYLMNEMHFSLSKS 82
           +E+ SVDWRGRPSNP  HGGMRAA FVLGLQAFEIM IAAVGNNLITY++NEMHF LSK+
Sbjct: 25  AEEESVDWRGRPSNPNKHGGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSKA 84

Query: 83  ANIVTNFVGTVFLLALLGGFLSDSYLGSFWTILIFGFVELS 124
           ANIVTNFVGT+F+ ALLGG+LSD++LGSFWTI+IFGFVELS
Sbjct: 85  ANIVTNFVGTIFIFALLGGYLSDAFLGSFWTIIIFGFVELS 125

[Close]

BLAST of Lsi05G007570 vs. TAIR10
Match: AT1G33440.1 (AT1G33440.1 Major facilitator superfamily protein)

HSP 1 Score: 155.2 bits (391), Expect = 2.6e-38
Identity = 79/107 (73.83%), Postives = 93/107 (86.92%), Query Frame = 1


Query: 18  HTNIPS-EDVSVDWRGRPSNPTSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYLMNEMH 77
           HT+  S +D+ VD+RGRP  P+ HGG RAA FVLG QAFE+MAIAAVGNNLITY+ NEMH
Sbjct: 16  HTSEESLDDLCVDFRGRPCRPSKHGGTRAALFVLGFQAFEMMAIAAVGNNLITYVFNEMH 75

Query: 78  FSLSKSANIVTNFVGTVFLLALLGGFLSDSYLGSFWTILIFGFVELS 124
           F LSKSAN+VTNF+GTVFLL+LLGGFLSDSYLGSF T+L+FG +E+S
Sbjct: 76  FPLSKSANLVTNFIGTVFLLSLLGGFLSDSYLGSFRTMLVFGVIEIS 122

[Close]

BLAST of Lsi05G007570 vs. TAIR10
Match: AT2G26690.1 (AT2G26690.1 Major facilitator superfamily protein)

HSP 1 Score: 94.4 bits (233), Expect = 5.5e-20
Identity = 48/95 (50.53%), Postives = 67/95 (70.53%), Query Frame = 1


Query: 27  SVDWRGRPSNPTSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYLMNEMHFSLSKSANIV 86
           +VD++GRP++ +  GG   AA +LG++  E ++   +  NL+TYLM  MH   S SANIV
Sbjct: 12  AVDYKGRPADKSKTGGWITAALILGIEVVERLSTMGIAVNLVTYLMETMHLPSSTSANIV 71

Query: 87  TNFVGTVFLLALLGGFLSDSYLGSFWTILIFGFVE 122
           T+F+GT FLL LLGGFL+DS+LG F TI IF  ++
Sbjct: 72  TDFMGTSFLLCLLGGFLADSFLGRFKTIGIFSTIQ 106

[Close]

BLAST of Lsi05G007570 vs. TAIR10
Match: AT1G69850.1 (AT1G69850.1 nitrate transporter 1:2)

HSP 1 Score: 90.9 bits (224), Expect = 6.1e-19
Identity = 50/93 (53.76%), Postives = 62/93 (66.67%), Query Frame = 1


Query: 29  DWRGRPSNPTSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYLMNEMHFSLSKSANIVTN 88
           DWR R +    HGGM AA+FVL ++  E +A  A  +NL+ YL   MH S SKSAN VTN
Sbjct: 15  DWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMSPSKSANDVTN 74

Query: 89  FVGTVFLLALLGGFLSDSYLGSFWTILIFGFVE 122
           F+GT FLLALLGGFLSD++  +F   LI   +E
Sbjct: 75  FMGTAFLLALLGGFLSDAFFSTFQIFLISASIE 107

[Close]

BLAST of Lsi05G007570 vs. TAIR10
Match: AT1G27040.1 (AT1G27040.1 Major facilitator superfamily protein)

HSP 1 Score: 88.6 bits (218), Expect = 3.0e-18
Identity = 47/108 (43.52%), Postives = 69/108 (63.89%), Query Frame = 1


Query: 14  KVESHTNIPSEDVSVDWRGRPSNPTSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYLMN 73
           +VE H ++   +   DWR + +    HGGM AA+FVL ++  E +A  A  +NL+ YL N
Sbjct: 2   EVEMHGDVSKWEGYADWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKN 61

Query: 74  EMHFSLSKSANIVTNFVGTVFLLALLGGFLSDSYLGSFWTILIFGFVE 122
            MH SL++S++ VT F+ T FLLALLGGFL+D++  +F   LI   +E
Sbjct: 62  FMHMSLARSSSEVTTFMATAFLLALLGGFLADAFFSTFVIFLISASIE 109

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BLAST of Lsi05G007570 vs. NCBI nr
Match: gi|449431892|ref|XP_004133734.1| (PREDICTED: protein NRT1/ PTR FAMILY 4.3 [Cucumis sativus])

HSP 1 Score: 203.4 bits (516), Expect = 2.4e-49
Identity = 104/121 (85.95%), Postives = 111/121 (91.74%), Query Frame = 1


Query: 3   MEMERERKRDFKVESHTNIPSEDVSVDWRGRPSNPTSHGGMRAAAFVLGLQAFEIMAIAA 62
           MEMER++ RD K E+H     +DVSVDWRGRPSNPTSHGGMRAA FVLG+Q FEIMAIAA
Sbjct: 1   MEMERQKMRDLKGENHPIPSDQDVSVDWRGRPSNPTSHGGMRAAVFVLGMQTFEIMAIAA 60

Query: 63  VGNNLITYLMNEMHFSLSKSANIVTNFVGTVFLLALLGGFLSDSYLGSFWTILIFGFVEL 122
           VGNNLITYL+NEMHFSLSKSANIVTNFVGTVFLLALLGGFLSDSYLGSFWT+LIFGFVEL
Sbjct: 61  VGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALLGGFLSDSYLGSFWTLLIFGFVEL 120

Query: 123 S 124
           S
Sbjct: 121 S 121

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BLAST of Lsi05G007570 vs. NCBI nr
Match: gi|659134124|ref|XP_008467038.1| (PREDICTED: protein NRT1/ PTR FAMILY 4.3-like, partial [Cucumis melo])

HSP 1 Score: 198.7 bits (504), Expect = 5.9e-48
Identity = 102/121 (84.30%), Postives = 109/121 (90.08%), Query Frame = 1


Query: 3   MEMERERKRDFKVESHTNIPSEDVSVDWRGRPSNPTSHGGMRAAAFVLGLQAFEIMAIAA 62
           MEME ++ RD K E+H     +DVSVDWRGRPSNPT HGGMRAA FVLG+Q FEIMAIAA
Sbjct: 1   MEMESQKMRDLKGENHPIPSDQDVSVDWRGRPSNPTFHGGMRAAVFVLGMQTFEIMAIAA 60

Query: 63  VGNNLITYLMNEMHFSLSKSANIVTNFVGTVFLLALLGGFLSDSYLGSFWTILIFGFVEL 122
           VGNNLITYL+NEMHFSLSKSANIVTNFVGTVFLLALLGGFLSDSYLGSFWT+LIFGFVEL
Sbjct: 61  VGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALLGGFLSDSYLGSFWTLLIFGFVEL 120

Query: 123 S 124
           S
Sbjct: 121 S 121

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BLAST of Lsi05G007570 vs. NCBI nr
Match: gi|566168327|ref|XP_006385089.1| (proton-dependent oligopeptide transport family protein [Populus trichocarpa])

HSP 1 Score: 184.1 bits (466), Expect = 1.5e-43
Identity = 97/121 (80.17%), Postives = 109/121 (90.08%), Query Frame = 1


Query: 3   MEMERERKRDFKVESHTNIPSEDVSVDWRGRPSNPTSHGGMRAAAFVLGLQAFEIMAIAA 62
           M+ME  R+ D K ES   + SE+V+VDWRG+PSNP  HGGMRAAAFVLGLQAFEIMAIAA
Sbjct: 1   MDMEA-RRHDTKGES-VCVVSEEVTVDWRGKPSNPNKHGGMRAAAFVLGLQAFEIMAIAA 60

Query: 63  VGNNLITYLMNEMHFSLSKSANIVTNFVGTVFLLALLGGFLSDSYLGSFWTILIFGFVEL 122
           VGNNLITY++NEMHFSLSKSANIVTNFVGT+F+LALLGG+LSDSYLG FWT+LIFGFVEL
Sbjct: 61  VGNNLITYVINEMHFSLSKSANIVTNFVGTIFILALLGGYLSDSYLGCFWTMLIFGFVEL 119

Query: 123 S 124
           S
Sbjct: 121 S 119

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BLAST of Lsi05G007570 vs. NCBI nr
Match: gi|743849205|ref|XP_011028408.1| (PREDICTED: protein NRT1/ PTR FAMILY 4.3 [Populus euphratica])

HSP 1 Score: 183.3 bits (464), Expect = 2.6e-43
Identity = 96/122 (78.69%), Postives = 109/122 (89.34%), Query Frame = 1


Query: 2   EMEMERERKRDFKVESHTNIPSEDVSVDWRGRPSNPTSHGGMRAAAFVLGLQAFEIMAIA 61
           EM+ME  R+ D K ES   + SE+++VDWRG+PSNP  HGGMRAAAFVLGLQ FEIMAIA
Sbjct: 58  EMDMEA-RRHDTKGES-VCVMSEEITVDWRGKPSNPNKHGGMRAAAFVLGLQGFEIMAIA 117

Query: 62  AVGNNLITYLMNEMHFSLSKSANIVTNFVGTVFLLALLGGFLSDSYLGSFWTILIFGFVE 121
           AVGNNLITY++NEMHFSLSKSANIVTNFVGT+F+LALLGG+LSDSYLG FWT+LIFGFVE
Sbjct: 118 AVGNNLITYVINEMHFSLSKSANIVTNFVGTIFILALLGGYLSDSYLGCFWTMLIFGFVE 177

Query: 122 LS 124
           LS
Sbjct: 178 LS 177

[Close]

BLAST of Lsi05G007570 vs. NCBI nr
Match: gi|694427506|ref|XP_009341381.1| (PREDICTED: protein NRT1/ PTR FAMILY 4.3-like [Pyrus x bretschneideri])

HSP 1 Score: 183.3 bits (464), Expect = 2.6e-43
Identity = 94/122 (77.05%), Postives = 113/122 (92.62%), Query Frame = 1


Query: 3   MEMERERKRDFKVES-HTNIPSEDVSVDWRGRPSNPTSHGGMRAAAFVLGLQAFEIMAIA 62
           MEME +R+++ KVE+ +T+  +E+++VDWRGRPSNPT HGGMRAAAFVLGLQ+FEIM IA
Sbjct: 1   MEME-QRRQNHKVEAINTSDMAEEMTVDWRGRPSNPTKHGGMRAAAFVLGLQSFEIMGIA 60

Query: 63  AVGNNLITYLMNEMHFSLSKSANIVTNFVGTVFLLALLGGFLSDSYLGSFWTILIFGFVE 122
           AVGNNLITY++NEMHF LS SAN+VTNF+GTVFLLAL+GG+LSDSYLGSFWT+LIFGFVE
Sbjct: 61  AVGNNLITYVINEMHFPLSNSANVVTNFIGTVFLLALVGGYLSDSYLGSFWTMLIFGFVE 120

Query: 123 LS 124
           LS
Sbjct: 121 LS 121

The following BLAST results are available for this feature:

BLAST of Lsi05G007570 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PTR16_ARATH	6.3e-42	82.18	Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana GN=NPF4.3 PE=2 SV=1	[more]
PTR15_ARATH	4.7e-37	73.83	Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana GN=NPF4.4 PE=2 SV=1	[more]
PTR27_ARATH	9.8e-19	50.53	Protein NRT1/ PTR FAMILY 6.2 OS=Arabidopsis thaliana GN=NPF6.2 PE=1 SV=1	[more]
PTR19_ARATH	1.1e-17	53.76	Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana GN=NPF4.6 PE=1 SV=1	[more]
PTR12_ARATH	5.4e-17	43.52	Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana GN=NPF4.5 PE=2 SV=1	[more]

BLAST of Lsi05G007570 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L2Q8_CUCSA	1.7e-49	85.95	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G112250 PE=4 SV=1	[more]
U5GKK1_POPTR	1.1e-43	80.17	Proton-dependent oligopeptide transport family protein OS=Populus trichocarpa GN...	[more]
M5XC53_PRUPE	3.1e-43	76.61	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa002962mg PE=4 SV=1	[more]
A0A061G9D1_THECC	6.8e-43	77.42	Major facilitator superfamily protein OS=Theobroma cacao GN=TCM_027118 PE=4 SV=1	[more]
B9SPB0_RICCO	4.4e-42	74.80	Nitrate transporter, putative OS=Ricinus communis GN=RCOM_0496050 PE=4 SV=1	[more]

BLAST of Lsi05G007570 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G59740.1	3.6e-43	82.18	Major facilitator superfamily protein	[more]
AT1G33440.1	2.6e-38	73.83	Major facilitator superfamily protein	[more]
AT2G26690.1	5.5e-20	50.53	Major facilitator superfamily protein	[more]
AT1G69850.1	6.1e-19	53.76	nitrate transporter 1:2	[more]
AT1G27040.1	3.0e-18	43.52	Major facilitator superfamily protein	[more]

BLAST of Lsi05G007570 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449431892\|ref\|XP_004133734.1\|	2.4e-49	85.95	PREDICTED: protein NRT1/ PTR FAMILY 4.3 [Cucumis sativus]	[more]
gi\|659134124\|ref\|XP_008467038.1\|	5.9e-48	84.30	PREDICTED: protein NRT1/ PTR FAMILY 4.3-like, partial [Cucumis melo]	[more]
gi\|566168327\|ref\|XP_006385089.1\|	1.5e-43	80.17	proton-dependent oligopeptide transport family protein [Populus trichocarpa]	[more]
gi\|743849205\|ref\|XP_011028408.1\|	2.6e-43	78.69	PREDICTED: protein NRT1/ PTR FAMILY 4.3 [Populus euphratica]	[more]
gi\|694427506\|ref\|XP_009341381.1\|	2.6e-43	77.05	PREDICTED: protein NRT1/ PTR FAMILY 4.3-like [Pyrus x bretschneideri]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Cellular Component**
Term	Definition
GO:0016020	membrane

Vocabulary: **Biological Process**
Term	Definition
GO:0006810	transport

Vocabulary: **Molecular Function**
Term	Definition
GO:0005215	transporter activity

Vocabulary: **INTERPRO**
Term	Definition
IPR020846	MFS_dom
IPR000109	POT_fam

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006810	transport
biological_process	GO:0006857	oligopeptide transport
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0005215	transporter activity
molecular_function	GO:0022857	transmembrane transporter activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G007570.1	Lsi05G007570.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000109	Proton-dependent oligopeptide transporter family	PANTHER	PTHR11654	OLIGOPEPTIDE TRANSPORTER-RELATED	coord: 1..123 score: 1.6
IPR020846	Major facilitator superfamily domain	unknown	SSF103473	MFS general substrate transporter	coord: 32..118 score: 1.3
None	No IPR available	GENE3D	G3DSA:1.20.1250.20		coord: 45..119 score: 7.
None	No IPR available	PANTHER	PTHR11654:SF177	PROTEIN NRT1/ PTR FAMILY 4.3	coord: 1..123 score: 1.6

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi05G007570	Bhi11G000682	Wax gourd	lsiwgoB410
Lsi05G007570	Cp4.1LG14g00580	Cucurbita pepo (Zucchini)	cpelsiB176
Lsi05G007570	Cp4.1LG01g08690	Cucurbita pepo (Zucchini)	cpelsiB370
Lsi05G007570	CsGy3G006610	Cucumber (Gy14) v2	cgyblsiB183
Lsi05G007570	Carg25875	Silver-seed gourd	carlsiB008
Lsi05G007570	Carg25372	Silver-seed gourd	carlsiB509
Lsi05G007570	CsaV3_3G006650	Cucumber (Chinese Long) v3	cuclsiB217

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi05G007570	Cucumber (Gy14) v1	cgylsiB159
Lsi05G007570	Cucurbita maxima (Rimu)	cmalsiB315
Lsi05G007570	Cucurbita moschata (Rifu)	cmolsiB305
Lsi05G007570	Wild cucumber (PI 183967)	cpilsiB197
Lsi05G007570	Cucumber (Chinese Long) v2	culsiB198
Lsi05G007570	Melon (DHL92) v3.5.1	lsimeB380
Lsi05G007570	Watermelon (Charleston Gray)	lsiwcgB318
Lsi05G007570	Cucumber (Gy14) v2	cgyblsiB203
Lsi05G007570	Melon (DHL92) v3.6.1	lsimedB387
Lsi05G007570	Watermelon (97103) v2	lsiwmbB323
Lsi05G007570	Watermelon (97103) v2	lsiwmbB322
Lsi05G007570	Wax gourd	lsiwgoB414