Lsi05G005260 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi05G005260
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionPutative serine/threonine-protein kinase/receptor R818
Locationchr05 : 6578882 .. 6581387 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATTGGCCACTGGCAGATCCCTTCAGCTCTCCCACCTTTCTTTTTCTTCTTCATTTTCCTTTCAATTTGCAGTTTCTTCTCTCAAGATTTCCCTTCAACTTCATCAACAGCTTCCAAGTTTATGGTACCCTATTTCTTTATTTTTATTCTTTTTATTCAAGCATCAAAATATTCTGTTTCTTTAATTTTTCTATAATAGCCTCAATATCTCTGGGTTTTTTATTGCTTTATCCATAGCTTAATTCTTGTCTTCCACAGAAAATTTTGCTTTCTGGGTATGAATTCTGATCTGGTTTCTTTTTAGTTTCATCTTTTTAGCAATCTTTTAATCAAATTCAGGAAAAAAAAGTACAAAATCACTAATTTTACTTGTTACTTACTAGTTACTTTTCCAGCAAGATTATCATTACTATTCTTTCATGATGTTATAGTTGAAATTTCATTGTATACATTCAAAAATTTAGGCCCGTTTGGTAATTATTTCGTGTTTGGTTTTTGGTTTCTGAGCAGTTGAATTCTTAGCCAAGTTTTAGAGGCAAAAAGCGTGTTTATAAGCCTAATTTTAAAAAAATACAAAACTAAACATAAGAGGGCCTTAACTTTTCTTATTTTACATTTAAAATTTGAATTTAAGTTATATGATATAGTATGAGATGTTTTGTTTGTTCAGATGCCTAGTGTGATGACAGAGTCATCAGATAAGGAGACAGAGGCATTTGTAGAGGTGGATCCGACGGGTCGATACGGGCGATACGTCGAGCTTCTGGGAACAGGGGCAGTGAAGAAAGTGTACAGAGCATTTGATCAAGAAGAGGGAATAGAAGTAGCTTGGAACCAAGTGAAACTAAGGAGTTTTTCAAATGATCCATCCATGATAGATAGGTTGTATTCAGAGGTGAGATTGTTAAGAACCTTAAAGAACAACAACATAATTGCCTTATATGATGTTTGGCTTGACAAACTTCATGGAACCTTAAATTTCATTACTGAGGTTTGTACAAGTGGGAACCTTAGAGAGTACAGGAAGAAACATAGACAAGTCTCTTTGAAAGCTTTGAAGAAATGGTCCAAACAAATTCTCAAGGGTTTGCATTATTTGCATACTCATGACCCTTGTGTTATTCATAGAGATCTCAATTGTAGCAACCTCTTTGTCAATGGCAATGTTGGCCAGGTATATACATTCTCTACTCTTATCTTTATTATCTTCTTTATTTTTCTTAGATCTAGGGGAGGAATAAGGCAAGGACATGTTTATTAAAATATTTATCCTTTTTATTACAAGAACGAAAGTCAAAGTCAATGAGTTTATACTTTTAGGGGGTGTTTGGTGCTCTGAGTTGTGTTATGAAATATTTTAAAGTTAGGAAGTTGGGGTGCAGAGTTGTTTAGTTGGAGTTAGGAAATCTATGTTTAGGAGTAGAGTTTTAGTCTGAAGTTAGGAAAACATTAAAGGGAGGGAGAGAGAAAGAGGGGAAGGTAAAGTTCATCATAAATGTGCAAACTTGAATAGTGAACACTGTTGAAGTTGGTAGAGTTGAGTTGTTTACCGCCGATTTATGAAGTCGGTGGGCCAACATCTCTTGTTCTTAAAAAAATAGTTTGATGTATTCCTGCTTTCAAAGTTTATTGTTTTCGTCCCATATGTATGTCTATTTAGTATACTTAATAAAGTGTCAAAGTGTTCAAGTGTCCAACACGTGTCAGATACAAACACATCGAGTTCAAAACCGTTCAAACATGAACGTTTATGATGTCATTTAGTACATTTGATCTTATAATTTGGCTTTGGGCATGGCAGGTAAAGATTGGTGACTTGGGTTTGGCAGCAACAGTGAGAAAGAATCACTCAGCTCATTCAGTGCTTGGAACACCAGAATTCATGGCACCTGAGTTATATGAAGAACATTACACTGAGCTTGTGGACATATATTCATTTGGAATGTGTTTGCTTGAATTGGTCACTTTGGAAATTCCCTATAGTGAATGTGACAATGTTGCCAAAATCTACAAGAAAGTCTCCTCAGGCATCAAGCCTCAAGCCCTTGACAAGGTCAAGGACCCTGAGGTCAAGGCTTTCATTGAGAATTGCCTTGCCGAATCCCGAACTCGGCCCTCGGCCGCTGACCTCCTTCGCCACCCCTTTTTTGGAGAAATTGATGATGACGAAAACGACGACAACAATGATCATTAGCGAATTGTGTTCGAATAAAAATTTAGATGTGGTTTATATATATATATATATATATATATATGGTGAATGAAAATTAGTACTCAAAGTAGGCTAGAATTGGGTTGTGCTTTTGTGGTTTGTTCTCATTGAAGTTCTTTTTTCTTTTATTTTTTTTTATTATGTGCTATCTTTTAATTATTTATAGAGAGGTAGCATGTGGGTTTGAAATGCTTTTCCCATATTTTTTAGGTTTTTATTTTATTTATGTTTATTTCTTGTACATAGACACATTAGTTTAATTCCAATATCACACTTTAAGAAGTTTTAGTTTAA

mRNA sequence

AATTGGCCACTGGCAGATCCCTTCAGCTCTCCCACCTTTCTTTTTCTTCTTCATTTTCCTTTCAATTTGCAGTTTCTTCTCTCAAGATTTCCCTTCAACTTCATCAACAGCTTCCAAGTTTATGATGCCTAGTGTGATGACAGAGTCATCAGATAAGGAGACAGAGGCATTTGTAGAGGTGGATCCGACGGGTCGATACGGGCGATACGTCGAGCTTCTGGGAACAGGGGCAGTGAAGAAAGTGTACAGAGCATTTGATCAAGAAGAGGGAATAGAAGTAGCTTGGAACCAAGTGAAACTAAGGAGTTTTTCAAATGATCCATCCATGATAGATAGGTTGTATTCAGAGGTGAGATTGTTAAGAACCTTAAAGAACAACAACATAATTGCCTTATATGATGTTTGGCTTGACAAACTTCATGGAACCTTAAATTTCATTACTGAGGTTTGTACAAGTGGGAACCTTAGAGAGTACAGGAAGAAACATAGACAAGTCTCTTTGAAAGCTTTGAAGAAATGGTCCAAACAAATTCTCAAGGGTTTGCATTATTTGCATACTCATGACCCTTGTGTTATTCATAGAGATCTCAATTGTAGCAACCTCTTTGTAAAGATTGGTGACTTGGGTTTGGCAGCAACAGTGAGAAAGAATCACTCAGCTCATTCAGTGCTTGGAACACCAGAATTCATGGCACCTGAGTTATATGAAGAACATTACACTGAGCTTGTGGACATATATTCATTTGGAATGTGTTTGCTTGAATTGGTCACTTTGGAAATTCCCTATAGTGAATGTGACAATGTTGCCAAAATCTACAAGAAAGTCTCCTCAGGCATCAAGCCTCAAGCCCTTGACAAGGTCAAGGACCCTGAGGTCAAGGCTTTCATTGAGAATTGCCTTGCCGAATCCCGAACTCGGCCCTCGGCCGCTGACCTCCTTCGCCACCCCTTTTTTGGAGAAATTGATGATGACGAAAACGACGACAACAATGATCATTAGCGAATTGTGTTCGAATAAAAATTTAGATGTGGTTTATATATATATATATATATATATATATGGTGAATGAAAATTAGTACTCAAAGTAGGCTAGAATTGGGTTGTGCTTTTGTGGTTTGTTCTCATTGAAGTTCTTTTTTCTTTTATTTTTTTTTATTATGTGCTATCTTTTAATTATTTATAGAGAGGTAGCATGTGGGTTTGAAATGCTTTTCCCATATTTTTTAGGTTTTTATTTTATTTATGTTTATTTCTTGTACATAGACACATTAGTTTAATTCCAATATCACACTTTAAGAAGTTTTAGTTTAA

Coding sequence (CDS)

ATGATGCCTAGTGTGATGACAGAGTCATCAGATAAGGAGACAGAGGCATTTGTAGAGGTGGATCCGACGGGTCGATACGGGCGATACGTCGAGCTTCTGGGAACAGGGGCAGTGAAGAAAGTGTACAGAGCATTTGATCAAGAAGAGGGAATAGAAGTAGCTTGGAACCAAGTGAAACTAAGGAGTTTTTCAAATGATCCATCCATGATAGATAGGTTGTATTCAGAGGTGAGATTGTTAAGAACCTTAAAGAACAACAACATAATTGCCTTATATGATGTTTGGCTTGACAAACTTCATGGAACCTTAAATTTCATTACTGAGGTTTGTACAAGTGGGAACCTTAGAGAGTACAGGAAGAAACATAGACAAGTCTCTTTGAAAGCTTTGAAGAAATGGTCCAAACAAATTCTCAAGGGTTTGCATTATTTGCATACTCATGACCCTTGTGTTATTCATAGAGATCTCAATTGTAGCAACCTCTTTGTAAAGATTGGTGACTTGGGTTTGGCAGCAACAGTGAGAAAGAATCACTCAGCTCATTCAGTGCTTGGAACACCAGAATTCATGGCACCTGAGTTATATGAAGAACATTACACTGAGCTTGTGGACATATATTCATTTGGAATGTGTTTGCTTGAATTGGTCACTTTGGAAATTCCCTATAGTGAATGTGACAATGTTGCCAAAATCTACAAGAAAGTCTCCTCAGGCATCAAGCCTCAAGCCCTTGACAAGGTCAAGGACCCTGAGGTCAAGGCTTTCATTGAGAATTGCCTTGCCGAATCCCGAACTCGGCCCTCGGCCGCTGACCTCCTTCGCCACCCCTTTTTTGGAGAAATTGATGATGACGAAAACGACGACAACAATGATCATTAG

Protein sequence

MMPSVMTESSDKETEAFVEVDPTGRYGRYVELLGTGAVKKVYRAFDQEEGIEVAWNQVKLRSFSNDPSMIDRLYSEVRLLRTLKNNNIIALYDVWLDKLHGTLNFITEVCTSGNLREYRKKHRQVSLKALKKWSKQILKGLHYLHTHDPCVIHRDLNCSNLFVKIGDLGLAATVRKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKKVSSGIKPQALDKVKDPEVKAFIENCLAESRTRPSAADLLRHPFFGEIDDDENDDNNDH
BLAST of Lsi05G005260 vs. Swiss-Prot
Match: WNK11_ARATH (Probable serine/threonine-protein kinase WNK11 OS=Arabidopsis thaliana GN=WNK11 PE=2 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 1.6e-128
Identity = 224/291 (76.98%), Postives = 257/291 (88.32%), Query Frame = 1

Query: 11  DKETEAFVEVDPTGRYGRYVELLGTGAVKKVYRAFDQEEGIEVAWNQVKLRSFSNDPSMI 70
           DK++E+FVEVDPTGRYGRY ELLG+GAVKKVYRAFDQEEGIEVAWNQVKLR FS+DP+M 
Sbjct: 15  DKDSESFVEVDPTGRYGRYGELLGSGAVKKVYRAFDQEEGIEVAWNQVKLRCFSDDPAMT 74

Query: 71  DRLYSEVRLLRTLKNNNIIALYDVWLDKLHGTLNFITEVCTSGNLREYRKKHRQVSLKAL 130
           +RLYSEVRLL+ LKN+NII LY VW D+ + TLNFITE+CTSGNLREYRKKHR VS++AL
Sbjct: 75  ERLYSEVRLLKNLKNSNIITLYKVWRDERNNTLNFITEICTSGNLREYRKKHRHVSMRAL 134

Query: 131 KKWSKQILKGLHYLHTHDPCVIHRDLNCSNLF-------VKIGDLGLAATVRKNHSAHSV 190
           KKWSKQILKGL YLHTHDPC+IHRDLNCSN+F       VKIGDLGLAA V KNH AHS+
Sbjct: 135 KKWSKQILKGLDYLHTHDPCIIHRDLNCSNIFVNGNIGQVKIGDLGLAAIVGKNHLAHSI 194

Query: 191 LGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKKVSSGIKPQA 250
           LGTPEFMAPELYEE+YTE+VDIYS+GMC+LELV+LEIPYSECD+VAKIYK+VS G+KP+A
Sbjct: 195 LGTPEFMAPELYEENYTEMVDIYSYGMCVLELVSLEIPYSECDSVAKIYKRVSKGLKPEA 254

Query: 251 LDKVKDPEVKAFIENCLAESRTRPSAADLLRHPFFGEI--DDDENDDNNDH 293
           L+KV DPE KAFIE C+A+ R RPSAA+LL  PFF  I  DDDE+ +NND+
Sbjct: 255 LNKVNDPEAKAFIEKCIAQPRARPSAAELLCDPFFDGILDDDDEDGENNDN 305

BLAST of Lsi05G005260 vs. Swiss-Prot
Match: WNK5_ORYSJ (Probable serine/threonine-protein kinase WNK5 OS=Oryza sativa subsp. japonica GN=WNK5 PE=2 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 2.8e-109
Identity = 187/289 (64.71%), Postives = 239/289 (82.70%), Query Frame = 1

Query: 6   MTESSDKETEAFVEVDPTGRYGRYVELLGTGAVKKVYRAFDQEEGIEVAWNQVKLRSFSN 65
           ++ S+ +E EAF EVDPTGR+GRY ++LG G+VKKVYR FDQEEGIEVAWN+V+LR+ ++
Sbjct: 34  VSASAGEEEEAFEEVDPTGRFGRYADVLGLGSVKKVYRGFDQEEGIEVAWNRVRLRALAD 93

Query: 66  -DPSMIDRLYSEVRLLRTLKNNNIIALYDVWLDKLHGTLNFITEVCTSGNLREYRKKHRQ 125
            DP+M++RL++EVRLLR+L + +II  + VWLD+  G LNFITEVCTSG+LREYR +HR 
Sbjct: 94  RDPAMVERLHAEVRLLRSLHHEHIIGFHKVWLDRDAGVLNFITEVCTSGSLREYRDRHRH 153

Query: 126 VSLKALKKWSKQILKGLHYLHTHDPCVIHRDLNCSNLF-------VKIGDLGLAATVRKN 185
           VS+KALKKW++QIL GL +LHTHDPC+IHRDLNCSN+F       VKIGDLGLAA V K 
Sbjct: 154 VSVKALKKWARQILLGLDHLHTHDPCIIHRDLNCSNVFINGNTGQVKIGDLGLAAIVDKT 213

Query: 186 HSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKKVSS 245
           H AH++LGTPEFMAPELY E YTE VDIYS+GMC+LE+VT E+PY+ECD+V +IY  V+ 
Sbjct: 214 HVAHTILGTPEFMAPELYTETYTESVDIYSYGMCVLEMVTREMPYAECDSVVQIYHSVTR 273

Query: 246 GIKPQALDKVKDPEVKAFIENCLAESRTRPSAADLLRHPFFGEIDDDEN 287
           G+ P AL +++DPE++AFIE C+ + R RPSAA+LLR PFF  IDDD++
Sbjct: 274 GVPPAALKRIRDPELRAFIERCIGQPRNRPSAAELLRDPFFAGIDDDDS 322

BLAST of Lsi05G005260 vs. Swiss-Prot
Match: WNK3_ARATH (Probable serine/threonine-protein kinase WNK3 OS=Arabidopsis thaliana GN=WNK3 PE=2 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 4.5e-99
Identity = 182/279 (65.23%), Postives = 212/279 (75.99%), Query Frame = 1

Query: 6   MTESSDKETEAFVEVDPTGRYGRYVELLGTGAVKKVYRAFDQEEGIEVAWNQVKLRSFSN 65
           M +  +   E FVE+DPTGRYGRY E+LG GA K+VYRAFDQ EGIEVAWNQVKL     
Sbjct: 1   MRQDENNSEEEFVEIDPTGRYGRYKEVLGKGAFKEVYRAFDQLEGIEVAWNQVKLDDKFC 60

Query: 66  DPSMIDRLYSEVRLLRTLKNNNIIALYDVWLDKLHGTLNFITEVCTSGNLREYRKKHRQV 125
               +DRLYSEV LL+TLK+ +II  Y  W+D  H T+N ITEV TSGNLR+YRKKH+ V
Sbjct: 61  SSEDLDRLYSEVHLLKTLKHKSIIKFYTSWIDHQHMTINLITEVFTSGNLRQYRKKHKCV 120

Query: 126 SLKALKKWSKQILKGLHYLHTHDPCVIHRDLNCSNLF-------VKIGDLGLAATVRKNH 185
            L+ALKKWS+QIL+GL YLH+HDP VIHRDL C N+F       VKIGDLGLAA + +  
Sbjct: 121 DLRALKKWSRQILEGLVYLHSHDPPVIHRDLKCDNIFINGNQGEVKIGDLGLAAILHRAR 180

Query: 186 SAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKKVSSG 245
           SAHSV+GTPEFMAPELYEE Y  LVDIY+FGMCLLELVT E PYSEC N A+IY+KV+SG
Sbjct: 181 SAHSVIGTPEFMAPELYEEDYNVLVDIYAFGMCLLELVTFEYPYSECTNAAQIYRKVTSG 240

Query: 246 IKPQALDKVKDPEVKAFIENCLAESRTRPSAADLLRHPF 278
           IKP AL  V DP+V+AFIE C+A+   R SA +LL  PF
Sbjct: 241 IKPAALLNVTDPQVRAFIEKCIAKVSQRLSAKELLDDPF 279

BLAST of Lsi05G005260 vs. Swiss-Prot
Match: WNK1_ARATH (Serine/threonine-protein kinase WNK1 OS=Arabidopsis thaliana GN=WNK1 PE=1 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 1.2e-96
Identity = 177/278 (63.67%), Postives = 212/278 (76.26%), Query Frame = 1

Query: 17  FVEVDPTGRYGRYVELLGTGAVKKVYRAFDQEEGIEVAWNQVKLRSFSNDPSMIDRLYSE 76
           FVEVDPTGRYGRY E+LG GA K VYRAFD+ EGIEVAWNQVKL  F   P  ++RLY E
Sbjct: 14  FVEVDPTGRYGRYNEVLGKGASKTVYRAFDEYEGIEVAWNQVKLYDFLQSPEDLERLYCE 73

Query: 77  VRLLRTLKNNNIIALYDVWLDKLHGTLNFITEVCTSGNLREYRKKHRQVSLKALKKWSKQ 136
           + LL+TLK+ NI+  Y  W+D  +  +NF+TE+ TSG LR+YR +H++V+++A+K W +Q
Sbjct: 74  IHLLKTLKHKNIMKFYTSWVDTANRNINFVTELFTSGTLRQYRLRHKRVNIRAMKHWCRQ 133

Query: 137 ILKGLHYLHTHDPCVIHRDLNCSNLF-------VKIGDLGLAATVRKNHSAHSVLGTPEF 196
           IL+GLHYLH+HDP VIHRDL C N+F       VKIGDLGLAA +RK+H+AH V GTPEF
Sbjct: 134 ILRGLHYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAAILRKSHAAHCV-GTPEF 193

Query: 197 MAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKKVSSGIKPQALDKVKD 256
           MAPE+YEE Y ELVDIYSFGMC+LE+VT + PYSEC + A+IYKKV SG KP AL KVKD
Sbjct: 194 MAPEVYEEAYNELVDIYSFGMCILEMVTFDYPYSECTHPAQIYKKVMSGKKPDALYKVKD 253

Query: 257 PEVKAFIENCLAESRTRPSAADLLRHPFFGEIDDDEND 288
           PEVK FIE CLA    R SA +LL  PF   IDD E D
Sbjct: 254 PEVKCFIEKCLATVSLRVSARELLDDPFL-RIDDGEFD 289

BLAST of Lsi05G005260 vs. Swiss-Prot
Match: WNK2_ORYSJ (Probable serine/threonine-protein kinase WNK2 OS=Oryza sativa subsp. japonica GN=WNK2 PE=2 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 1.1e-94
Identity = 175/282 (62.06%), Postives = 210/282 (74.47%), Query Frame = 1

Query: 11  DKETEAFVEVDPTGRYGRYVELLGTGAVKKVYRAFDQEEGIEVAWNQVKLRSFSNDPSMI 70
           D E E F EVDPT RYGRY E+LG GA K VY+AFDQ EG+EVAWNQ+K+     +   +
Sbjct: 13  DTEPE-FAEVDPTARYGRYTEVLGKGAFKTVYKAFDQLEGLEVAWNQIKVGDILRNNDDL 72

Query: 71  DRLYSEVRLLRTLKNNNIIALYDVWLDKLHGTLNFITEVCTSGNLREYRKKHRQVSLKAL 130
           +RL SEVRLL+TLK+ NII  Y+ WLDK +  +NFITEV TSG LR+YR KH++V ++AL
Sbjct: 73  ERLRSEVRLLKTLKHKNIIKFYNSWLDKKNNNINFITEVFTSGTLRQYRIKHKKVDVRAL 132

Query: 131 KKWSKQILKGLHYLHTHDPCVIHRDLNCSNLF-------VKIGDLGLAATVRKNHSAHSV 190
           KKWS+QIL GL YLH+HDP VIHRDL C N+F       VKIGDLGLA  +    SAHS+
Sbjct: 133 KKWSRQILSGLVYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLATILDNARSAHSI 192

Query: 191 LGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKKVSSGIKPQA 250
           +GTPEFMAPELY+E Y ELVDIY+FGMCLLELVT E PY EC N A+IYKKVS G KP +
Sbjct: 193 IGTPEFMAPELYDEEYNELVDIYAFGMCLLELVTFEYPYCECSNAAQIYKKVSDGEKPSS 252

Query: 251 LDKVKDPEVKAFIENCLAESRTRPSAADLLRHPFFGEIDDDE 286
           L K++DPEV+ FIE C+A++  R SA +LL  PF    DD E
Sbjct: 253 LAKIEDPEVRFFIEKCIAKASQRLSAQELLMDPFLR--DDGE 291

BLAST of Lsi05G005260 vs. TrEMBL
Match: A0A0A0L4W9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G062560 PE=4 SV=1)

HSP 1 Score: 561.2 bits (1445), Expect = 7.3e-157
Identity = 283/300 (94.33%), Postives = 287/300 (95.67%), Query Frame = 1

Query: 1   MMPSVMTESSDKETEAFVEVDPTGRYGRYVELLGTGAVKKVYRAFDQEEGIEVAWNQVKL 60
           MMPSVMTESSDKETEAFVEVDPT RYGRY ELLGTGAVKKVYRAFDQEEGIEVAWNQVKL
Sbjct: 1   MMPSVMTESSDKETEAFVEVDPTCRYGRYPELLGTGAVKKVYRAFDQEEGIEVAWNQVKL 60

Query: 61  RSFSNDPSMIDRLYSEVRLLRTLKNNNIIALYDVWLDKLHGTLNFITEVCTSGNLREYRK 120
           RSFSNDPSMIDRLYSEVRLLR+LKNNNIIALYDVWLDKLHGTLNFITEVCTSGNLREYRK
Sbjct: 61  RSFSNDPSMIDRLYSEVRLLRSLKNNNIIALYDVWLDKLHGTLNFITEVCTSGNLREYRK 120

Query: 121 KHRQVSLKALKKWSKQILKGLHYLHTHDPCVIHRDLNCSNLF-------VKIGDLGLAAT 180
           KHRQVSLKALKKWSKQILKGLHYLH++DPCVIHRDLNCSNLF       VKIGDLGLAAT
Sbjct: 121 KHRQVSLKALKKWSKQILKGLHYLHSNDPCVIHRDLNCSNLFVNGNVGQVKIGDLGLAAT 180

Query: 181 VRKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYK 240
           VRKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYK
Sbjct: 181 VRKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYK 240

Query: 241 KVSSGIKPQALDKVKDPEVKAFIENCLAESRTRPSAADLLRHPFFGEIDDDEN-DDNNDH 293
           KVSSGIKPQAL KVKDPEVKAFIENCLAES+ RPSAADLLRHPFF EIDDDEN DDNNDH
Sbjct: 241 KVSSGIKPQALGKVKDPEVKAFIENCLAESKVRPSAADLLRHPFFREIDDDENEDDNNDH 300

BLAST of Lsi05G005260 vs. TrEMBL
Match: A0A067L435_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_00992 PE=4 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 2.7e-135
Identity = 236/298 (79.19%), Postives = 269/298 (90.27%), Query Frame = 1

Query: 2   MPSVMTESSDKETEAFVEVDPTGRYGRYVELLGTGAVKKVYRAFDQEEGIEVAWNQVKLR 61
           MPSV ++SSD++ E FVE+DPTGRYGRY ELLG+GAVKKVYRAFDQEEGIEVAWNQVKLR
Sbjct: 1   MPSVSSDSSDQDAEPFVEIDPTGRYGRYNELLGSGAVKKVYRAFDQEEGIEVAWNQVKLR 60

Query: 62  SFSNDPSMIDRLYSEVRLLRTLKNNNIIALYDVWLDKLHGTLNFITEVCTSGNLREYRKK 121
           +F++DP+MIDRLYSEVRLLR+L N NII+LY+VW D+ H TLNFITEVCTSGNLREYRKK
Sbjct: 61  NFTSDPAMIDRLYSEVRLLRSLTNKNIISLYNVWRDEDHNTLNFITEVCTSGNLREYRKK 120

Query: 122 HRQVSLKALKKWSKQILKGLHYLHTHDPCVIHRDLNCSNLF-------VKIGDLGLAATV 181
           HR VS+KALKKWS+QILKGL+YLHTH+PC+IHRDLNCSNLF       VKIGDLGLAA V
Sbjct: 121 HRHVSIKALKKWSRQILKGLNYLHTHEPCIIHRDLNCSNLFINGNIGQVKIGDLGLAAIV 180

Query: 182 RKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKK 241
            K HSAHS+LGTPEFMAPELYEEHYTE+VDIYSFGMC+LE+VTL+IPYSECDNVA+IYKK
Sbjct: 181 GKGHSAHSILGTPEFMAPELYEEHYTEMVDIYSFGMCVLEMVTLQIPYSECDNVARIYKK 240

Query: 242 VSSGIKPQALDKVKDPEVKAFIENCLAESRTRPSAADLLRHPFFGEIDDDENDDNNDH 293
           VSSGI+PQAL KV+DPEV+AFIE CLA+ R RPSAADLL+ PFF  IDDD+ +D+N H
Sbjct: 241 VSSGIRPQALSKVRDPEVRAFIERCLAQPRARPSAADLLKDPFFDGIDDDDENDDNAH 298

BLAST of Lsi05G005260 vs. TrEMBL
Match: A0A0D2Q373_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_002G096100 PE=4 SV=1)

HSP 1 Score: 488.4 bits (1256), Expect = 6.0e-135
Identity = 235/294 (79.93%), Postives = 267/294 (90.82%), Query Frame = 1

Query: 1   MMPSVMTESSDKETEAFVEVDPTGRYGRYVELLGTGAVKKVYRAFDQEEGIEVAWNQVKL 60
           MMPSV  + SDK++E FVEVDPTGRYGRY ELLG+GAVKKVYRAFDQEEGIEVAWNQVKL
Sbjct: 1   MMPSVNPDQSDKDSEPFVEVDPTGRYGRYTELLGSGAVKKVYRAFDQEEGIEVAWNQVKL 60

Query: 61  RSFSNDPSMIDRLYSEVRLLRTLKNNNIIALYDVWLDKLHGTLNFITEVCTSGNLREYRK 120
           R+FS+DP+MIDRLYSEVRLLR+L NNNII+LY  W D+ H TLNFITEVCTSGNLREYRK
Sbjct: 61  RNFSDDPAMIDRLYSEVRLLRSLTNNNIISLYSFWRDEEHNTLNFITEVCTSGNLREYRK 120

Query: 121 KHRQVSLKALKKWSKQILKGLHYLHTHDPCVIHRDLNCSNLF-------VKIGDLGLAAT 180
           KHRQVS+KALKKWSKQILKGL+YLH+H+PC+IHRDLNCSN+F       VKIGDLGLAA 
Sbjct: 121 KHRQVSMKALKKWSKQILKGLNYLHSHEPCIIHRDLNCSNVFVNGNTGQVKIGDLGLAAI 180

Query: 181 VRKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYK 240
           V KNHSAHS+LGTPEFMAPELY+EHYTEL+DIYSFGMC+LE+VTLEIPYSECDNVAKIYK
Sbjct: 181 VGKNHSAHSILGTPEFMAPELYDEHYTELIDIYSFGMCVLEMVTLEIPYSECDNVAKIYK 240

Query: 241 KVSSGIKPQALDKVKDPEVKAFIENCLAESRTRPSAADLLRHPFFGEIDD-DEN 287
           KVSSG+KPQALDKV+D +++AFIE C+A+ R RPSAA+LL+ PF+ E+DD DEN
Sbjct: 241 KVSSGVKPQALDKVRDADMRAFIERCIAQPRERPSAAELLKDPFYDEVDDYDEN 294

BLAST of Lsi05G005260 vs. TrEMBL
Match: A0A0B0MVN5_GOSAR (Putative serine/threonine-protein kinase WNK11 OS=Gossypium arboreum GN=F383_26191 PE=4 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 1.7e-134
Identity = 234/297 (78.79%), Postives = 267/297 (89.90%), Query Frame = 1

Query: 2   MPSVMTESSDKETEAFVEVDPTGRYGRYVELLGTGAVKKVYRAFDQEEGIEVAWNQVKLR 61
           MP V  + SDK++E FVE+DPTGRYGRY ELLG+GAVKKVYRAFDQEEGIEVAWNQVKLR
Sbjct: 1   MPGVNQDQSDKDSEPFVEIDPTGRYGRYSELLGSGAVKKVYRAFDQEEGIEVAWNQVKLR 60

Query: 62  SFSNDPSMIDRLYSEVRLLRTLKNNNIIALYDVWLDKLHGTLNFITEVCTSGNLREYRKK 121
           +FSNDPSMIDRLYSEVRLLR+L N NII+LY+VW D+ H TLNFITEVCTSGNLREYRKK
Sbjct: 61  NFSNDPSMIDRLYSEVRLLRSLTNRNIISLYNVWRDEEHNTLNFITEVCTSGNLREYRKK 120

Query: 122 HRQVSLKALKKWSKQILKGLHYLHTHDPCVIHRDLNCSNLF-------VKIGDLGLAATV 181
           HRQVS+KALKKWSKQILKGL YLHTHDPC+IHRDLNCSN+F       VKIGDLG+AA V
Sbjct: 121 HRQVSIKALKKWSKQILKGLDYLHTHDPCIIHRDLNCSNVFVNGNTGQVKIGDLGMAAIV 180

Query: 182 RKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKK 241
            K+HSAHS+LGTPEFMAPELYEEHYTEL+DIYSFGMCLLE+VTLEIPYSEC+NV KIYKK
Sbjct: 181 GKSHSAHSILGTPEFMAPELYEEHYTELIDIYSFGMCLLEMVTLEIPYSECENVGKIYKK 240

Query: 242 VSSGIKPQALDKVKDPEVKAFIENCLAESRTRPSAADLLRHPFFGEIDDDENDDNND 292
           VSSG++PQA+ KV+D +VK FIE CLA+ R+RPSAA+LL+ PFF E+ DD++DD++D
Sbjct: 241 VSSGVRPQAMSKVRDEDVKEFIERCLAQPRSRPSAAELLKDPFFYEVVDDDDDDDDD 297

BLAST of Lsi05G005260 vs. TrEMBL
Match: A0A0D2M8R1_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_002G096100 PE=4 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 1.7e-134
Identity = 234/293 (79.86%), Postives = 266/293 (90.78%), Query Frame = 1

Query: 2   MPSVMTESSDKETEAFVEVDPTGRYGRYVELLGTGAVKKVYRAFDQEEGIEVAWNQVKLR 61
           MPSV  + SDK++E FVEVDPTGRYGRY ELLG+GAVKKVYRAFDQEEGIEVAWNQVKLR
Sbjct: 3   MPSVNPDQSDKDSEPFVEVDPTGRYGRYTELLGSGAVKKVYRAFDQEEGIEVAWNQVKLR 62

Query: 62  SFSNDPSMIDRLYSEVRLLRTLKNNNIIALYDVWLDKLHGTLNFITEVCTSGNLREYRKK 121
           +FS+DP+MIDRLYSEVRLLR+L NNNII+LY  W D+ H TLNFITEVCTSGNLREYRKK
Sbjct: 63  NFSDDPAMIDRLYSEVRLLRSLTNNNIISLYSFWRDEEHNTLNFITEVCTSGNLREYRKK 122

Query: 122 HRQVSLKALKKWSKQILKGLHYLHTHDPCVIHRDLNCSNLF-------VKIGDLGLAATV 181
           HRQVS+KALKKWSKQILKGL+YLH+H+PC+IHRDLNCSN+F       VKIGDLGLAA V
Sbjct: 123 HRQVSMKALKKWSKQILKGLNYLHSHEPCIIHRDLNCSNVFVNGNTGQVKIGDLGLAAIV 182

Query: 182 RKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKK 241
            KNHSAHS+LGTPEFMAPELY+EHYTEL+DIYSFGMC+LE+VTLEIPYSECDNVAKIYKK
Sbjct: 183 GKNHSAHSILGTPEFMAPELYDEHYTELIDIYSFGMCVLEMVTLEIPYSECDNVAKIYKK 242

Query: 242 VSSGIKPQALDKVKDPEVKAFIENCLAESRTRPSAADLLRHPFFGEIDD-DEN 287
           VSSG+KPQALDKV+D +++AFIE C+A+ R RPSAA+LL+ PF+ E+DD DEN
Sbjct: 243 VSSGVKPQALDKVRDADMRAFIERCIAQPRERPSAAELLKDPFYDEVDDYDEN 295

BLAST of Lsi05G005260 vs. TAIR10
Match: AT5G55560.1 (AT5G55560.1 Protein kinase superfamily protein)

HSP 1 Score: 460.3 bits (1183), Expect = 8.9e-130
Identity = 224/291 (76.98%), Postives = 257/291 (88.32%), Query Frame = 1

Query: 11  DKETEAFVEVDPTGRYGRYVELLGTGAVKKVYRAFDQEEGIEVAWNQVKLRSFSNDPSMI 70
           DK++E+FVEVDPTGRYGRY ELLG+GAVKKVYRAFDQEEGIEVAWNQVKLR FS+DP+M 
Sbjct: 15  DKDSESFVEVDPTGRYGRYGELLGSGAVKKVYRAFDQEEGIEVAWNQVKLRCFSDDPAMT 74

Query: 71  DRLYSEVRLLRTLKNNNIIALYDVWLDKLHGTLNFITEVCTSGNLREYRKKHRQVSLKAL 130
           +RLYSEVRLL+ LKN+NII LY VW D+ + TLNFITE+CTSGNLREYRKKHR VS++AL
Sbjct: 75  ERLYSEVRLLKNLKNSNIITLYKVWRDERNNTLNFITEICTSGNLREYRKKHRHVSMRAL 134

Query: 131 KKWSKQILKGLHYLHTHDPCVIHRDLNCSNLF-------VKIGDLGLAATVRKNHSAHSV 190
           KKWSKQILKGL YLHTHDPC+IHRDLNCSN+F       VKIGDLGLAA V KNH AHS+
Sbjct: 135 KKWSKQILKGLDYLHTHDPCIIHRDLNCSNIFVNGNIGQVKIGDLGLAAIVGKNHLAHSI 194

Query: 191 LGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKKVSSGIKPQA 250
           LGTPEFMAPELYEE+YTE+VDIYS+GMC+LELV+LEIPYSECD+VAKIYK+VS G+KP+A
Sbjct: 195 LGTPEFMAPELYEENYTEMVDIYSYGMCVLELVSLEIPYSECDSVAKIYKRVSKGLKPEA 254

Query: 251 LDKVKDPEVKAFIENCLAESRTRPSAADLLRHPFFGEI--DDDENDDNNDH 293
           L+KV DPE KAFIE C+A+ R RPSAA+LL  PFF  I  DDDE+ +NND+
Sbjct: 255 LNKVNDPEAKAFIEKCIAQPRARPSAAELLCDPFFDGILDDDDEDGENNDN 305

BLAST of Lsi05G005260 vs. TAIR10
Match: AT3G48260.1 (AT3G48260.1 with no lysine (K) kinase 3)

HSP 1 Score: 362.5 bits (929), Expect = 2.5e-100
Identity = 182/279 (65.23%), Postives = 212/279 (75.99%), Query Frame = 1

Query: 6   MTESSDKETEAFVEVDPTGRYGRYVELLGTGAVKKVYRAFDQEEGIEVAWNQVKLRSFSN 65
           M +  +   E FVE+DPTGRYGRY E+LG GA K+VYRAFDQ EGIEVAWNQVKL     
Sbjct: 1   MRQDENNSEEEFVEIDPTGRYGRYKEVLGKGAFKEVYRAFDQLEGIEVAWNQVKLDDKFC 60

Query: 66  DPSMIDRLYSEVRLLRTLKNNNIIALYDVWLDKLHGTLNFITEVCTSGNLREYRKKHRQV 125
               +DRLYSEV LL+TLK+ +II  Y  W+D  H T+N ITEV TSGNLR+YRKKH+ V
Sbjct: 61  SSEDLDRLYSEVHLLKTLKHKSIIKFYTSWIDHQHMTINLITEVFTSGNLRQYRKKHKCV 120

Query: 126 SLKALKKWSKQILKGLHYLHTHDPCVIHRDLNCSNLF-------VKIGDLGLAATVRKNH 185
            L+ALKKWS+QIL+GL YLH+HDP VIHRDL C N+F       VKIGDLGLAA + +  
Sbjct: 121 DLRALKKWSRQILEGLVYLHSHDPPVIHRDLKCDNIFINGNQGEVKIGDLGLAAILHRAR 180

Query: 186 SAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKKVSSG 245
           SAHSV+GTPEFMAPELYEE Y  LVDIY+FGMCLLELVT E PYSEC N A+IY+KV+SG
Sbjct: 181 SAHSVIGTPEFMAPELYEEDYNVLVDIYAFGMCLLELVTFEYPYSECTNAAQIYRKVTSG 240

Query: 246 IKPQALDKVKDPEVKAFIENCLAESRTRPSAADLLRHPF 278
           IKP AL  V DP+V+AFIE C+A+   R SA +LL  PF
Sbjct: 241 IKPAALLNVTDPQVRAFIEKCIAKVSQRLSAKELLDDPF 279

BLAST of Lsi05G005260 vs. TAIR10
Match: AT3G04910.1 (AT3G04910.1 with no lysine (K) kinase 1)

HSP 1 Score: 354.4 bits (908), Expect = 6.8e-98
Identity = 177/278 (63.67%), Postives = 212/278 (76.26%), Query Frame = 1

Query: 17  FVEVDPTGRYGRYVELLGTGAVKKVYRAFDQEEGIEVAWNQVKLRSFSNDPSMIDRLYSE 76
           FVEVDPTGRYGRY E+LG GA K VYRAFD+ EGIEVAWNQVKL  F   P  ++RLY E
Sbjct: 14  FVEVDPTGRYGRYNEVLGKGASKTVYRAFDEYEGIEVAWNQVKLYDFLQSPEDLERLYCE 73

Query: 77  VRLLRTLKNNNIIALYDVWLDKLHGTLNFITEVCTSGNLREYRKKHRQVSLKALKKWSKQ 136
           + LL+TLK+ NI+  Y  W+D  +  +NF+TE+ TSG LR+YR +H++V+++A+K W +Q
Sbjct: 74  IHLLKTLKHKNIMKFYTSWVDTANRNINFVTELFTSGTLRQYRLRHKRVNIRAMKHWCRQ 133

Query: 137 ILKGLHYLHTHDPCVIHRDLNCSNLF-------VKIGDLGLAATVRKNHSAHSVLGTPEF 196
           IL+GLHYLH+HDP VIHRDL C N+F       VKIGDLGLAA +RK+H+AH V GTPEF
Sbjct: 134 ILRGLHYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAAILRKSHAAHCV-GTPEF 193

Query: 197 MAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKKVSSGIKPQALDKVKD 256
           MAPE+YEE Y ELVDIYSFGMC+LE+VT + PYSEC + A+IYKKV SG KP AL KVKD
Sbjct: 194 MAPEVYEEAYNELVDIYSFGMCILEMVTFDYPYSECTHPAQIYKKVMSGKKPDALYKVKD 253

Query: 257 PEVKAFIENCLAESRTRPSAADLLRHPFFGEIDDDEND 288
           PEVK FIE CLA    R SA +LL  PF   IDD E D
Sbjct: 254 PEVKCFIEKCLATVSLRVSARELLDDPFL-RIDDGEFD 289

BLAST of Lsi05G005260 vs. TAIR10
Match: AT5G28080.2 (AT5G28080.2 Protein kinase superfamily protein)

HSP 1 Score: 334.3 bits (856), Expect = 7.3e-92
Identity = 161/274 (58.76%), Postives = 206/274 (75.18%), Query Frame = 1

Query: 11  DKETEAFVEVDPTGRYGRYVELLGTGAVKKVYRAFDQEEGIEVAWNQVKLRSFSNDPSMI 70
           + +   +VEVDPTGRYGRY E+LG G+ K VYR FD+ +GIEVAWNQVKL  F   P  +
Sbjct: 9   ESDYSEYVEVDPTGRYGRYNEVLGKGSSKTVYRGFDEYQGIEVAWNQVKLYDFLQSPQEL 68

Query: 71  DRLYSEVRLLRTLKNNNIIALYDVWLDKLHGTLNFITEVCTSGNLREYRKKHRQVSLKAL 130
           +RLY E+ LL+TLK+ +I+  Y  W+D  +  +NF+TE+ TSG LR+YR KH++V+++A+
Sbjct: 69  ERLYCEIHLLKTLKHKSIMKFYASWVDTDNRNINFVTEMFTSGTLRQYRLKHKRVNIRAV 128

Query: 131 KKWSKQILKGLHYLHTHDPCVIHRDLNCSNLF-------VKIGDLGLAATVRKNHSAHSV 190
           K W +QIL+GL+YLHTHDP VIHRDL C N+F       VKIGDLGLAA ++ +H+AH V
Sbjct: 129 KNWCRQILRGLNYLHTHDPPVIHRDLKCDNIFINGNQGEVKIGDLGLAACLQHSHAAHCV 188

Query: 191 LGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKKVSSGIKPQA 250
            GTPEFMAPE+Y+E Y +LVDIYSFGMC+LE+VT + PYSEC + A+IYK+V SG KP  
Sbjct: 189 -GTPEFMAPEVYKEEYNQLVDIYSFGMCVLEMVTFDYPYSECSHPAQIYKRVISGKKPDG 248

Query: 251 LDKVKDPEVKAFIENCLAESRTRPSAADLLRHPF 278
           LDKVKDPEV+ FIE CLA    R SA +LL   F
Sbjct: 249 LDKVKDPEVRGFIEKCLATVSLRLSACELLDDHF 281

BLAST of Lsi05G005260 vs. TAIR10
Match: AT3G18750.1 (AT3G18750.1 with no lysine (K) kinase 6)

HSP 1 Score: 334.0 bits (855), Expect = 9.6e-92
Identity = 167/281 (59.43%), Postives = 210/281 (74.73%), Query Frame = 1

Query: 4   SVMTESSDKETEAFVEVDPTGRYGRYVELLGTGAVKKVYRAFDQEEGIEVAWNQVKLRSF 63
           S + E  D E    +EVDPT RY RY E++G GA K VY+AFD+ +GIEVAWNQV++   
Sbjct: 8   SALQEPPDPEV---LEVDPTFRYIRYKEVIGKGAFKTVYKAFDEVDGIEVAWNQVRIDDV 67

Query: 64  SNDPSMIDRLYSEVRLLRTLKNNNIIALYDVWLDKLHGTLNFITEVCTSGNLREYRKKHR 123
              P+ ++RLYSEVRLL++LK+NNII  Y+ W+D  + T+N ITE+ TSG+LR YRKKHR
Sbjct: 68  LQSPNCLERLYSEVRLLKSLKHNNIIRFYNSWIDDKNKTVNIITELFTSGSLRHYRKKHR 127

Query: 124 QVSLKALKKWSKQILKGLHYLHTHDPCVIHRDLNCSNLF-------VKIGDLGLAATVRK 183
           +V++KA+K W++QIL GL YLH  +P +IHRDL C N+F       VKIGDLGL ATV +
Sbjct: 128 KVNMKAVKNWARQILMGLRYLHGQEPPIIHRDLKCDNIFINGNHGEVKIGDLGL-ATVME 187

Query: 184 NHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKKVS 243
             +A SV+GTPEFMAPELY+E+Y EL DIYSFGMC+LE+VT + PY EC N A+IYKKVS
Sbjct: 188 QANAKSVIGTPEFMAPELYDENYNELADIYSFGMCMLEMVTFDYPYCECKNSAQIYKKVS 247

Query: 244 SGIKPQALDKVKDPEVKAFIENCLAESRTRPSAADLLRHPF 278
           SGIKP +L +VKDPEVK FIE CL  +  R SA +LL  PF
Sbjct: 248 SGIKPASLSRVKDPEVKQFIEKCLLPASERLSAKELLLDPF 284

BLAST of Lsi05G005260 vs. NCBI nr
Match: gi|449463721|ref|XP_004149580.1| (PREDICTED: probable serine/threonine-protein kinase WNK11 [Cucumis sativus])

HSP 1 Score: 561.2 bits (1445), Expect = 1.0e-156
Identity = 283/300 (94.33%), Postives = 287/300 (95.67%), Query Frame = 1

Query: 1   MMPSVMTESSDKETEAFVEVDPTGRYGRYVELLGTGAVKKVYRAFDQEEGIEVAWNQVKL 60
           MMPSVMTESSDKETEAFVEVDPT RYGRY ELLGTGAVKKVYRAFDQEEGIEVAWNQVKL
Sbjct: 1   MMPSVMTESSDKETEAFVEVDPTCRYGRYPELLGTGAVKKVYRAFDQEEGIEVAWNQVKL 60

Query: 61  RSFSNDPSMIDRLYSEVRLLRTLKNNNIIALYDVWLDKLHGTLNFITEVCTSGNLREYRK 120
           RSFSNDPSMIDRLYSEVRLLR+LKNNNIIALYDVWLDKLHGTLNFITEVCTSGNLREYRK
Sbjct: 61  RSFSNDPSMIDRLYSEVRLLRSLKNNNIIALYDVWLDKLHGTLNFITEVCTSGNLREYRK 120

Query: 121 KHRQVSLKALKKWSKQILKGLHYLHTHDPCVIHRDLNCSNLF-------VKIGDLGLAAT 180
           KHRQVSLKALKKWSKQILKGLHYLH++DPCVIHRDLNCSNLF       VKIGDLGLAAT
Sbjct: 121 KHRQVSLKALKKWSKQILKGLHYLHSNDPCVIHRDLNCSNLFVNGNVGQVKIGDLGLAAT 180

Query: 181 VRKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYK 240
           VRKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYK
Sbjct: 181 VRKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYK 240

Query: 241 KVSSGIKPQALDKVKDPEVKAFIENCLAESRTRPSAADLLRHPFFGEIDDDEN-DDNNDH 293
           KVSSGIKPQAL KVKDPEVKAFIENCLAES+ RPSAADLLRHPFF EIDDDEN DDNNDH
Sbjct: 241 KVSSGIKPQALGKVKDPEVKAFIENCLAESKVRPSAADLLRHPFFREIDDDENEDDNNDH 300

BLAST of Lsi05G005260 vs. NCBI nr
Match: gi|659096406|ref|XP_008449078.1| (PREDICTED: probable serine/threonine-protein kinase WNK11 [Cucumis melo])

HSP 1 Score: 560.8 bits (1444), Expect = 1.4e-156
Identity = 283/300 (94.33%), Postives = 286/300 (95.33%), Query Frame = 1

Query: 1   MMPSVMTESSDKETEAFVEVDPTGRYGRYVELLGTGAVKKVYRAFDQEEGIEVAWNQVKL 60
           MMPSVMTESSDKETEAFVEVDPTGRYGRY ELLGTGAVKKVYRAFDQEEGIEVAWNQVKL
Sbjct: 1   MMPSVMTESSDKETEAFVEVDPTGRYGRYPELLGTGAVKKVYRAFDQEEGIEVAWNQVKL 60

Query: 61  RSFSNDPSMIDRLYSEVRLLRTLKNNNIIALYDVWLDKLHGTLNFITEVCTSGNLREYRK 120
           RSFSNDPSMIDRLYSEVRLLR+LKNNNIIALYDVWLDKLHGTLNFITEVCTSGNLREYRK
Sbjct: 61  RSFSNDPSMIDRLYSEVRLLRSLKNNNIIALYDVWLDKLHGTLNFITEVCTSGNLREYRK 120

Query: 121 KHRQVSLKALKKWSKQILKGLHYLHTHDPCVIHRDLNCSNLF-------VKIGDLGLAAT 180
           KHRQVSLKALKKWSKQILKGL YLHT+DPCVIHRDLNCSNLF       VKIGDLGLAAT
Sbjct: 121 KHRQVSLKALKKWSKQILKGLQYLHTNDPCVIHRDLNCSNLFVNGNVGQVKIGDLGLAAT 180

Query: 181 VRKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYK 240
           +RKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYK
Sbjct: 181 IRKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYK 240

Query: 241 KVSSGIKPQALDKVKDPEVKAFIENCLAESRTRPSAADLLRHPFFGEIDDDEN-DDNNDH 293
           KVSSGIKPQAL KVKDPEVKAFIENCLAESR RPSAADLLRH FF EIDDDEN DDNNDH
Sbjct: 241 KVSSGIKPQALGKVKDPEVKAFIENCLAESRVRPSAADLLRHSFFREIDDDENEDDNNDH 300

BLAST of Lsi05G005260 vs. NCBI nr
Match: gi|802588578|ref|XP_012070964.1| (PREDICTED: probable serine/threonine-protein kinase WNK11 [Jatropha curcas])

HSP 1 Score: 491.5 bits (1264), Expect = 1.0e-135
Identity = 237/299 (79.26%), Postives = 270/299 (90.30%), Query Frame = 1

Query: 1   MMPSVMTESSDKETEAFVEVDPTGRYGRYVELLGTGAVKKVYRAFDQEEGIEVAWNQVKL 60
           MMPSV ++SSD++ E FVE+DPTGRYGRY ELLG+GAVKKVYRAFDQEEGIEVAWNQVKL
Sbjct: 1   MMPSVSSDSSDQDAEPFVEIDPTGRYGRYNELLGSGAVKKVYRAFDQEEGIEVAWNQVKL 60

Query: 61  RSFSNDPSMIDRLYSEVRLLRTLKNNNIIALYDVWLDKLHGTLNFITEVCTSGNLREYRK 120
           R+F++DP+MIDRLYSEVRLLR+L N NII+LY+VW D+ H TLNFITEVCTSGNLREYRK
Sbjct: 61  RNFTSDPAMIDRLYSEVRLLRSLTNKNIISLYNVWRDEDHNTLNFITEVCTSGNLREYRK 120

Query: 121 KHRQVSLKALKKWSKQILKGLHYLHTHDPCVIHRDLNCSNLF-------VKIGDLGLAAT 180
           KHR VS+KALKKWS+QILKGL+YLHTH+PC+IHRDLNCSNLF       VKIGDLGLAA 
Sbjct: 121 KHRHVSIKALKKWSRQILKGLNYLHTHEPCIIHRDLNCSNLFINGNIGQVKIGDLGLAAI 180

Query: 181 VRKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYK 240
           V K HSAHS+LGTPEFMAPELYEEHYTE+VDIYSFGMC+LE+VTL+IPYSECDNVA+IYK
Sbjct: 181 VGKGHSAHSILGTPEFMAPELYEEHYTEMVDIYSFGMCVLEMVTLQIPYSECDNVARIYK 240

Query: 241 KVSSGIKPQALDKVKDPEVKAFIENCLAESRTRPSAADLLRHPFFGEIDDDENDDNNDH 293
           KVSSGI+PQAL KV+DPEV+AFIE CLA+ R RPSAADLL+ PFF  IDDD+ +D+N H
Sbjct: 241 KVSSGIRPQALSKVRDPEVRAFIERCLAQPRARPSAADLLKDPFFDGIDDDDENDDNAH 299

BLAST of Lsi05G005260 vs. NCBI nr
Match: gi|643732043|gb|KDP39235.1| (hypothetical protein JCGZ_00992 [Jatropha curcas])

HSP 1 Score: 489.6 bits (1259), Expect = 3.9e-135
Identity = 236/298 (79.19%), Postives = 269/298 (90.27%), Query Frame = 1

Query: 2   MPSVMTESSDKETEAFVEVDPTGRYGRYVELLGTGAVKKVYRAFDQEEGIEVAWNQVKLR 61
           MPSV ++SSD++ E FVE+DPTGRYGRY ELLG+GAVKKVYRAFDQEEGIEVAWNQVKLR
Sbjct: 1   MPSVSSDSSDQDAEPFVEIDPTGRYGRYNELLGSGAVKKVYRAFDQEEGIEVAWNQVKLR 60

Query: 62  SFSNDPSMIDRLYSEVRLLRTLKNNNIIALYDVWLDKLHGTLNFITEVCTSGNLREYRKK 121
           +F++DP+MIDRLYSEVRLLR+L N NII+LY+VW D+ H TLNFITEVCTSGNLREYRKK
Sbjct: 61  NFTSDPAMIDRLYSEVRLLRSLTNKNIISLYNVWRDEDHNTLNFITEVCTSGNLREYRKK 120

Query: 122 HRQVSLKALKKWSKQILKGLHYLHTHDPCVIHRDLNCSNLF-------VKIGDLGLAATV 181
           HR VS+KALKKWS+QILKGL+YLHTH+PC+IHRDLNCSNLF       VKIGDLGLAA V
Sbjct: 121 HRHVSIKALKKWSRQILKGLNYLHTHEPCIIHRDLNCSNLFINGNIGQVKIGDLGLAAIV 180

Query: 182 RKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKK 241
            K HSAHS+LGTPEFMAPELYEEHYTE+VDIYSFGMC+LE+VTL+IPYSECDNVA+IYKK
Sbjct: 181 GKGHSAHSILGTPEFMAPELYEEHYTEMVDIYSFGMCVLEMVTLQIPYSECDNVARIYKK 240

Query: 242 VSSGIKPQALDKVKDPEVKAFIENCLAESRTRPSAADLLRHPFFGEIDDDENDDNNDH 293
           VSSGI+PQAL KV+DPEV+AFIE CLA+ R RPSAADLL+ PFF  IDDD+ +D+N H
Sbjct: 241 VSSGIRPQALSKVRDPEVRAFIERCLAQPRARPSAADLLKDPFFDGIDDDDENDDNAH 298

BLAST of Lsi05G005260 vs. NCBI nr
Match: gi|823132196|ref|XP_012461443.1| (PREDICTED: probable serine/threonine-protein kinase WNK11 [Gossypium raimondii])

HSP 1 Score: 488.4 bits (1256), Expect = 8.6e-135
Identity = 235/294 (79.93%), Postives = 267/294 (90.82%), Query Frame = 1

Query: 1   MMPSVMTESSDKETEAFVEVDPTGRYGRYVELLGTGAVKKVYRAFDQEEGIEVAWNQVKL 60
           MMPSV  + SDK++E FVEVDPTGRYGRY ELLG+GAVKKVYRAFDQEEGIEVAWNQVKL
Sbjct: 1   MMPSVNPDQSDKDSEPFVEVDPTGRYGRYTELLGSGAVKKVYRAFDQEEGIEVAWNQVKL 60

Query: 61  RSFSNDPSMIDRLYSEVRLLRTLKNNNIIALYDVWLDKLHGTLNFITEVCTSGNLREYRK 120
           R+FS+DP+MIDRLYSEVRLLR+L NNNII+LY  W D+ H TLNFITEVCTSGNLREYRK
Sbjct: 61  RNFSDDPAMIDRLYSEVRLLRSLTNNNIISLYSFWRDEEHNTLNFITEVCTSGNLREYRK 120

Query: 121 KHRQVSLKALKKWSKQILKGLHYLHTHDPCVIHRDLNCSNLF-------VKIGDLGLAAT 180
           KHRQVS+KALKKWSKQILKGL+YLH+H+PC+IHRDLNCSN+F       VKIGDLGLAA 
Sbjct: 121 KHRQVSMKALKKWSKQILKGLNYLHSHEPCIIHRDLNCSNVFVNGNTGQVKIGDLGLAAI 180

Query: 181 VRKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYK 240
           V KNHSAHS+LGTPEFMAPELY+EHYTEL+DIYSFGMC+LE+VTLEIPYSECDNVAKIYK
Sbjct: 181 VGKNHSAHSILGTPEFMAPELYDEHYTELIDIYSFGMCVLEMVTLEIPYSECDNVAKIYK 240

Query: 241 KVSSGIKPQALDKVKDPEVKAFIENCLAESRTRPSAADLLRHPFFGEIDD-DEN 287
           KVSSG+KPQALDKV+D +++AFIE C+A+ R RPSAA+LL+ PF+ E+DD DEN
Sbjct: 241 KVSSGVKPQALDKVRDADMRAFIERCIAQPRERPSAAELLKDPFYDEVDDYDEN 294

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
WNK11_ARATH1.6e-12876.98Probable serine/threonine-protein kinase WNK11 OS=Arabidopsis thaliana GN=WNK11 ... [more]
WNK5_ORYSJ2.8e-10964.71Probable serine/threonine-protein kinase WNK5 OS=Oryza sativa subsp. japonica GN... [more]
WNK3_ARATH4.5e-9965.23Probable serine/threonine-protein kinase WNK3 OS=Arabidopsis thaliana GN=WNK3 PE... [more]
WNK1_ARATH1.2e-9663.67Serine/threonine-protein kinase WNK1 OS=Arabidopsis thaliana GN=WNK1 PE=1 SV=1[more]
WNK2_ORYSJ1.1e-9462.06Probable serine/threonine-protein kinase WNK2 OS=Oryza sativa subsp. japonica GN... [more]
Match NameE-valueIdentityDescription
A0A0A0L4W9_CUCSA7.3e-15794.33Uncharacterized protein OS=Cucumis sativus GN=Csa_3G062560 PE=4 SV=1[more]
A0A067L435_JATCU2.7e-13579.19Uncharacterized protein OS=Jatropha curcas GN=JCGZ_00992 PE=4 SV=1[more]
A0A0D2Q373_GOSRA6.0e-13579.93Uncharacterized protein OS=Gossypium raimondii GN=B456_002G096100 PE=4 SV=1[more]
A0A0B0MVN5_GOSAR1.7e-13478.79Putative serine/threonine-protein kinase WNK11 OS=Gossypium arboreum GN=F383_261... [more]
A0A0D2M8R1_GOSRA1.7e-13479.86Uncharacterized protein OS=Gossypium raimondii GN=B456_002G096100 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G55560.18.9e-13076.98 Protein kinase superfamily protein[more]
AT3G48260.12.5e-10065.23 with no lysine (K) kinase 3[more]
AT3G04910.16.8e-9863.67 with no lysine (K) kinase 1[more]
AT5G28080.27.3e-9258.76 Protein kinase superfamily protein[more]
AT3G18750.19.6e-9259.43 with no lysine (K) kinase 6[more]
Match NameE-valueIdentityDescription
gi|449463721|ref|XP_004149580.1|1.0e-15694.33PREDICTED: probable serine/threonine-protein kinase WNK11 [Cucumis sativus][more]
gi|659096406|ref|XP_008449078.1|1.4e-15694.33PREDICTED: probable serine/threonine-protein kinase WNK11 [Cucumis melo][more]
gi|802588578|ref|XP_012070964.1|1.0e-13579.26PREDICTED: probable serine/threonine-protein kinase WNK11 [Jatropha curcas][more]
gi|643732043|gb|KDP39235.1|3.9e-13579.19hypothetical protein JCGZ_00992 [Jatropha curcas][more]
gi|823132196|ref|XP_012461443.1|8.6e-13579.93PREDICTED: probable serine/threonine-protein kinase WNK11 [Gossypium raimondii][more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0006468protein phosphorylation
Vocabulary: Molecular Function
TermDefinition
GO:0005524ATP binding
GO:0004672protein kinase activity
Vocabulary: INTERPRO
TermDefinition
IPR011009Kinase-like_dom_sf
IPR000719Prot_kinase_dom
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000186 activation of MAPKK activity
biological_process GO:0009069 serine family amino acid metabolic process
biological_process GO:0007178 transmembrane receptor protein serine/threonine kinase signaling pathway
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005622 intracellular
cellular_component GO:0005575 cellular_component
molecular_function GO:0005524 ATP binding
molecular_function GO:0004709 MAP kinase kinase kinase activity
molecular_function GO:0004672 protein kinase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G005260.1Lsi05G005260.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 30..278
score: 2.4
IPR000719Protein kinase domainPROFILEPS50011PROTEIN_KINASE_DOMcoord: 27..278
score: 36
IPR011009Protein kinase-like domainunknownSSF56112Protein kinase-like (PK-like)coord: 29..283
score: 9.84
NoneNo IPR availableGENE3DG3DSA:1.10.510.10coord: 128..289
score: 2.2
NoneNo IPR availableGENE3DG3DSA:3.30.200.20coord: 12..127
score: 5.3
NoneNo IPR availablePANTHERPTHR13902SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATEDcoord: 8..290
score: 2.6E
NoneNo IPR availablePANTHERPTHR13902:SF44SERINE/THREONINE-PROTEIN KINASE WNK11-RELATEDcoord: 8..290
score: 2.6E