Lsi05G004710 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi05G004710
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Lysosomal cystine transporter family protein
Location	chr05 : 5970882 .. 5971595 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATATCCTCCAAAAAAAAAAAAAAATCTCCAAGAGGCGTCAGTCTCATCATACAATTAGTTCATCTCCTCACAGAAACTGGGAAGAGAGAGAAAGTAGAGGGAGAGAGAGAAATGGCTTCATGGAATTCAATTTCAATGGAGATCATATACCAGATTTTAGGATGGAGTTCCATCATATGCTGGGCGCTTGCTGCATATCCACAGGTCATTCTGAATTTCCGGCGAAAAAGGTAATTAACAAATCAAATCTTAATCGTCGATTCCAGCACCGCCGTCGTAATCCTCTTCTTCTTCTTCTTCTCCGGTGGAGAAATTAAAGAGGACTGATATTCAGATTTTGATTTGGTAGTGTTGTAGGATTGAGCTTCGATTACGTGATTCTGAATTTCACGAAGCAATCGTCGTATTTGATATACAACGCTACTCTGTTCTTCAGCGCCACTGTTCAGAGGCAGTACTTCGACAAATACGGCTACGATCAGGTTGGCCTTCTTCTTTCTTCCTTCCACTTCTCTTCAGATTTTTGTTTCAATACATTGCGATTCGTTGATGATCCATTCTATATCTATATCTTCAACTGAATTGAAAACGGCTTGATTAGGTTTTTTTCGTCTTGTTTCTCTCGCCAATCCGAACTTCGAATCGGTGATTATTGCGAAACTGCGGAAATCTTTCGTTCCTTACATTTTCTTTCGATTCTTTTCTTCGAGGTAG

mRNA sequence

ATATCCTCCAAAAAAAAAAAAAAATCTCCAAGAGGCGTCAGTCTCATCATACAATTAGTTCATCTCCTCACAGAAACTGGGAAGAGAGAGAAAGTAGAGGGAGAGAGAGAAATGGCTTCATGGAATTCAATTTCAATGGAGATCATATACCAGATTTTAGGATGGAGTTCCATCATATGCTGGGCGCTTGCTGCATATCCACAGGTCATTCTGAATTTCCGGCGAAAAAGGATTGAGCTTCGATTACGTGATTCTGAATTTCACGAAGCAATCGTCGTATTTGATATACAACGCTACTCTGTTCTTCAGCGCCACTGTTCAGAGGCAGTACTTCGACAAATACGGCTACGATCAGGTTTTTTTCGTCTTGTTTCTCTCGCCAATCCGAACTTCGAATCGGTGATTATTGCGAAACTGCGGAAATCTTTCGTTCCTTACATTTTCTTTCGATTCTTTTCTTCGAGGTAG

Coding sequence (CDS)

ATGGCTTCATGGAATTCAATTTCAATGGAGATCATATACCAGATTTTAGGATGGAGTTCCATCATATGCTGGGCGCTTGCTGCATATCCACAGGTCATTCTGAATTTCCGGCGAAAAAGGATTGAGCTTCGATTACGTGATTCTGAATTTCACGAAGCAATCGTCGTATTTGATATACAACGCTACTCTGTTCTTCAGCGCCACTGTTCAGAGGCAGTACTTCGACAAATACGGCTACGATCAGGTTTTTTTCGTCTTGTTTCTCTCGCCAATCCGAACTTCGAATCGGTGATTATTGCGAAACTGCGGAAATCTTTCGTTCCTTACATTTTCTTTCGATTCTTTTCTTCGAGGTAG

Protein sequence

MASWNSISMEIIYQILGWSSIICWALAAYPQVILNFRRKRIELRLRDSEFHEAIVVFDIQRYSVLQRHCSEAVLRQIRLRSGFFRLVSLANPNFESVIIAKLRKSFVPYIFFRFFSSR

Homology

[Close]

BLAST of Lsi05G004710 vs. Swiss-Prot
Match: CTNS_ARATH (Cystinosin homolog OS=Arabidopsis thaliana GN=At5g40670 PE=2 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 7.3e-08
Identity = 23/41 (56.10%), Postives = 33/41 (80.49%), Query Frame = 1


Query: 1  MASWNSISMEIIYQILGWSSIICWALAAYPQVILNFRRKRI 42
          MASWNSI +EI Y+I+GW +   W+++ YPQ+ILNFRR+ +
Sbjct: 1  MASWNSIPLEISYEIVGWIAFASWSISFYPQLILNFRRRSV 41

[Close]

BLAST of Lsi05G004710 vs. TrEMBL
Match: A0A0A0LB92_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G596240 PE=4 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 6.4e-11
Identity = 32/41 (78.05%), Postives = 38/41 (92.68%), Query Frame = 1


Query: 1  MASWNSISMEIIYQILGWSSIICWALAAYPQVILNFRRKRI 42
          M+SWNSISMEI+Y +LGW SI+CWALA+YPQVILNFRRK +
Sbjct: 1  MSSWNSISMEILYGVLGWISILCWALASYPQVILNFRRKSV 41

[Close]

BLAST of Lsi05G004710 vs. TrEMBL
Match: E5GCE0_CUCME (Lysosomal cystine transporter family protein OS=Cucumis melo subsp. melo PE=4 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 1.6e-09
Identity = 28/41 (68.29%), Postives = 35/41 (85.37%), Query Frame = 1


Query: 1  MASWNSISMEIIYQILGWSSIICWALAAYPQVILNFRRKRI 42
          M SWNSI ME++YQ+LGW SIICWA + YPQ++LNFRRK +
Sbjct: 1  MGSWNSIPMEVMYQVLGWISIICWAFSGYPQLLLNFRRKSV 41

[Close]

BLAST of Lsi05G004710 vs. TrEMBL
Match: F6GX62_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0219g00120 PE=4 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 4.6e-09
Identity = 35/95 (36.84%), Postives = 58/95 (61.05%), Query Frame = 1


Query: 1  MASWNSISMEIIYQILGWSSIICWALAAYPQVILNFRRKRI-----ELRLRDSEFHEAIV 60
          MASWNS S+EI+YQ+ GW++ + W+++ YPQVILNFRRK +     +  + +   H + +
Sbjct: 1  MASWNSTSLEIVYQVFGWTAFVSWSISFYPQVILNFRRKSVVGLNFDFVVLNLTKHSSYL 60

Query: 61 VFDIQRYSVLQRHCSEAVLRQIRLRSGFFRLVSLA 91
          +++   Y       S AV RQ R + G  +++ +A
Sbjct: 61 IYNAVLY------FSSAVQRQYREKYGIGQMIPVA 89

[Close]

BLAST of Lsi05G004710 vs. TrEMBL
Match: I3T233_MEDTR (Lysosomal cystine transporter family protein OS=Medicago truncatula GN=MTR_0018s0320 PE=2 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 2.3e-08
Identity = 33/95 (34.74%), Postives = 58/95 (61.05%), Query Frame = 1


Query: 1  MASWNSISMEIIYQILGWSSIICWALAAYPQVILNFRRKRI-----ELRLRDSEFHEAIV 60
          MASWNS+S+E+ Y++LGW + I W+++ YPQVILNFRRK +     +  L +   H + +
Sbjct: 1  MASWNSVSLEVTYEVLGWFAFIVWSISFYPQVILNFRRKSVVGLNFDFVLLNLTKHSSYL 60

Query: 61 VFDIQRYSVLQRHCSEAVLRQIRLRSGFFRLVSLA 91
          +++   Y       S A+ +Q   + G+ +++ +A
Sbjct: 61 IYNSSLY------FSSAIQKQYFQKYGYDQMIPVA 89

[Close]

BLAST of Lsi05G004710 vs. TrEMBL
Match: A0A059AGN1_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_J02273 PE=4 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 3.0e-08
Identity = 34/95 (35.79%), Postives = 58/95 (61.05%), Query Frame = 1


Query: 1  MASWNSISMEIIYQILGWSSIICWALAAYPQVILNFRRKRI-----ELRLRDSEFHEAIV 60
          MASWNS S+E++Y++LGWS+   W+++ YPQVILNFRRK +     +  + +   H + +
Sbjct: 1  MASWNSTSLEVLYEVLGWSAFASWSVSFYPQVILNFRRKSVVGLNFDFVVLNLTKHSSYL 60

Query: 61 VFDIQRYSVLQRHCSEAVLRQIRLRSGFFRLVSLA 91
          +++   Y       S AV RQ   + G+ +++ +A
Sbjct: 61 IYNATLY------FSAAVQRQYYEKYGYGQMIPVA 89

[Close]

BLAST of Lsi05G004710 vs. TAIR10
Match: AT5G40670.1 (AT5G40670.1 PQ-loop repeat family protein / transmembrane family protein)

HSP 1 Score: 58.2 bits (139), Expect = 4.1e-09
Identity = 23/41 (56.10%), Postives = 33/41 (80.49%), Query Frame = 1


Query: 1  MASWNSISMEIIYQILGWSSIICWALAAYPQVILNFRRKRI 42
          MASWNSI +EI Y+I+GW +   W+++ YPQ+ILNFRR+ +
Sbjct: 1  MASWNSIPLEISYEIVGWIAFASWSISFYPQLILNFRRRSV 41

[Close]

BLAST of Lsi05G004710 vs. NCBI nr
Match: gi|449455900|ref|XP_004145688.1| (PREDICTED: cystinosin homolog isoform X2 [Cucumis sativus])

HSP 1 Score: 75.1 bits (183), Expect = 9.2e-11
Identity = 32/41 (78.05%), Postives = 38/41 (92.68%), Query Frame = 1


Query: 1  MASWNSISMEIIYQILGWSSIICWALAAYPQVILNFRRKRI 42
          M+SWNSISMEI+Y +LGW SI+CWALA+YPQVILNFRRK +
Sbjct: 1  MSSWNSISMEILYGVLGWISILCWALASYPQVILNFRRKSV 41

[Close]

BLAST of Lsi05G004710 vs. NCBI nr
Match: gi|778681772|ref|XP_011651580.1| (PREDICTED: cystinosin homolog isoform X1 [Cucumis sativus])

HSP 1 Score: 75.1 bits (183), Expect = 9.2e-11
Identity = 32/41 (78.05%), Postives = 38/41 (92.68%), Query Frame = 1


Query: 1  MASWNSISMEIIYQILGWSSIICWALAAYPQVILNFRRKRI 42
          M+SWNSISMEI+Y +LGW SI+CWALA+YPQVILNFRRK +
Sbjct: 1  MSSWNSISMEILYGVLGWISILCWALASYPQVILNFRRKSV 41

[Close]

BLAST of Lsi05G004710 vs. NCBI nr
Match: gi|307136316|gb|ADN34139.1| (lysosomal cystine transporter family protein [Cucumis melo subsp. melo])

HSP 1 Score: 70.5 bits (171), Expect = 2.3e-09
Identity = 28/41 (68.29%), Postives = 35/41 (85.37%), Query Frame = 1


Query: 1  MASWNSISMEIIYQILGWSSIICWALAAYPQVILNFRRKRI 42
          M SWNSI ME++YQ+LGW SIICWA + YPQ++LNFRRK +
Sbjct: 1  MGSWNSIPMEVMYQVLGWISIICWAFSGYPQLLLNFRRKSV 41

[Close]

BLAST of Lsi05G004710 vs. NCBI nr
Match: gi|659098212|ref|XP_008450030.1| (PREDICTED: cystinosin homolog isoform X2 [Cucumis melo])

HSP 1 Score: 70.5 bits (171), Expect = 2.3e-09
Identity = 28/41 (68.29%), Postives = 35/41 (85.37%), Query Frame = 1


Query: 1  MASWNSISMEIIYQILGWSSIICWALAAYPQVILNFRRKRI 42
          M SWNSI ME++YQ+LGW SIICWA + YPQ++LNFRRK +
Sbjct: 1  MGSWNSIPMEVMYQVLGWISIICWAFSGYPQLLLNFRRKSV 41

[Close]

BLAST of Lsi05G004710 vs. NCBI nr
Match: gi|659098210|ref|XP_008450029.1| (PREDICTED: cystinosin homolog isoform X1 [Cucumis melo])

HSP 1 Score: 70.5 bits (171), Expect = 2.3e-09
Identity = 28/41 (68.29%), Postives = 35/41 (85.37%), Query Frame = 1


Query: 1  MASWNSISMEIIYQILGWSSIICWALAAYPQVILNFRRKRI 42
          M SWNSI ME++YQ+LGW SIICWA + YPQ++LNFRRK +
Sbjct: 1  MGSWNSIPMEVMYQVLGWISIICWAFSGYPQLLLNFRRKSV 41

The following BLAST results are available for this feature:

BLAST of Lsi05G004710 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CTNS_ARATH	7.3e-08	56.10	Cystinosin homolog OS=Arabidopsis thaliana GN=At5g40670 PE=2 SV=1	[more]

BLAST of Lsi05G004710 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LB92_CUCSA	6.4e-11	78.05	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G596240 PE=4 SV=1	[more]
E5GCE0_CUCME	1.6e-09	68.29	Lysosomal cystine transporter family protein OS=Cucumis melo subsp. melo PE=4 SV...	[more]
F6GX62_VITVI	4.6e-09	36.84	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0219g00120 PE=4 SV=...	[more]
I3T233_MEDTR	2.3e-08	34.74	Lysosomal cystine transporter family protein OS=Medicago truncatula GN=MTR_0018s...	[more]
A0A059AGN1_EUCGR	3.0e-08	35.79	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_J02273 PE=4 SV=1	[more]

BLAST of Lsi05G004710 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G40670.1	4.1e-09	56.10	PQ-loop repeat family protein / transmembrane family protein	[more]

BLAST of Lsi05G004710 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449455900\|ref\|XP_004145688.1\|	9.2e-11	78.05	PREDICTED: cystinosin homolog isoform X2 [Cucumis sativus]	[more]
gi\|778681772\|ref\|XP_011651580.1\|	9.2e-11	78.05	PREDICTED: cystinosin homolog isoform X1 [Cucumis sativus]	[more]
gi\|307136316\|gb\|ADN34139.1\|	2.3e-09	68.29	lysosomal cystine transporter family protein [Cucumis melo subsp. melo]	[more]
gi\|659098212\|ref\|XP_008450030.1\|	2.3e-09	68.29	PREDICTED: cystinosin homolog isoform X2 [Cucumis melo]	[more]
gi\|659098210\|ref\|XP_008450029.1\|	2.3e-09	68.29	PREDICTED: cystinosin homolog isoform X1 [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR006603	PQ-loop_rpt

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G004710.1	Lsi05G004710.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006603	PQ-loop repeat	PFAM	PF04193	PQ-loop	coord: 13..40 score: 1.

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi05G004710	MELO3C014821	Melon (DHL92) v3.5.1	lsimeB368
Lsi05G004710	ClCG05G012970	Watermelon (Charleston Gray)	lsiwcgB332
Lsi05G004710	Bhi01G001554	Wax gourd	lsiwgoB436

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi05G004710	Watermelon (97103) v2	lsiwmbB329
Lsi05G004710	Cucumber (Gy14) v1	cgylsiB248
Lsi05G004710	Cucurbita maxima (Rimu)	cmalsiB125
Lsi05G004710	Cucurbita maxima (Rimu)	cmalsiB248
Lsi05G004710	Cucurbita moschata (Rifu)	cmolsiB117
Lsi05G004710	Cucurbita moschata (Rifu)	cmolsiB236
Lsi05G004710	Cucurbita pepo (Zucchini)	cpelsiB509
Lsi05G004710	Cucurbita pepo (Zucchini)	cpelsiB511
Lsi05G004710	Cucurbita pepo (Zucchini)	cpelsiB555
Lsi05G004710	Wild cucumber (PI 183967)	cpilsiB212
Lsi05G004710	Cucumber (Chinese Long) v2	culsiB214
Lsi05G004710	Cucumber (Gy14) v2	cgyblsiB201
Lsi05G004710	Melon (DHL92) v3.6.1	lsimedB402
Lsi05G004710	Silver-seed gourd	carlsiB334
Lsi05G004710	Silver-seed gourd	carlsiB396
Lsi05G004710	Cucumber (Chinese Long) v3	cuclsiB233