Lsi05G004670 (gene) Bottle gourd (USVL1VR-Ls)

BLAST of Lsi05G004670 vs. Swiss-Prot
Match: SEOB_ARATH (Protein SIEVE ELEMENT OCCLUSION B OS=Arabidopsis thaliana GN=SEOB PE=1 SV=1)

HSP 1 Score: 675.6 bits (1742), Expect = 6.1e-193
Identity = 352/716 (49.16%), Postives = 485/716 (67.74%), Query Frame = 1

Query: 44  LSAISDDNVMMKQIIATHDPDDRDVDTRSLLRLVENILKRATLAADATVWNESLPTKQGS 103
           L+  SD+++M+K I  TH PD R+V  R LL LVE+IL RATL ++ T  N S+      
Sbjct: 34  LAMSSDESMMLKLIQQTHSPDAREVQVRGLLSLVEDILDRATLDSEDT--NASM------ 93

Query: 104 YEQLESLEETGTHQAGFTTMLETLSYTIDRISSEISYKALEGIDPHATTLAIFNMLASYR 163
              L    E    Q+   ++L+++SY IDR++ EI+YK+L G D H  T+++F  L+S++
Sbjct: 94  ---LPLPTEDKLMQSSMMSVLDSVSYAIDRVACEIAYKSLTGSDSHEITMSVFEHLSSFQ 153

Query: 164 WDAKLVLTLAAFALNYGEFWLLAQIYSENQLAKAMAILKQLPGIFEHSIALKPKFDALKD 223
           WD KLVLTLAAFALNYGEFWLL Q YS+NQLAK++A+LK +P   ++ + L+     L D
Sbjct: 154 WDGKLVLTLAAFALNYGEFWLLVQFYSKNQLAKSLAMLKLVP--VQNRVTLESVSQGLND 213

Query: 224 LVAAILDVTWCIIDLKELPSAYISQEVPAMSTAVAHIPTAVYWTIRSIVSSATQITSLTS 283
           L+  +  VT C+++L ELP  YI+ +VP +S  ++ IP AVYWTIRS+++  +QI  +T+
Sbjct: 214 LIREMKSVTACVVELSELPDRYITPDVPQLSRILSTIPIAVYWTIRSVIACISQINMITA 273

Query: 284 MGYELALSTSTDSWELSTLAHKLKNIRDHLKKKLVLCHQYIEEKRDIESFQMLINLLEMT 343
           MG+E+ ++T  D WE S LA+KLKNI DHL + L LC+++IE++R  ES ++L +L + T
Sbjct: 274 MGHEM-MNTQMDLWETSMLANKLKNIHDHLAETLRLCYRHIEKQRSSESLKVLHSLFDTT 333

Query: 344 HLDNMKVLKALIYAKDDLQSLVDGSTGQRVNLDVLKRKNVLLLISDLNISHDELSILDQL 403
           H+DNMK+L AL++ K  +  L DG T ++V+LDVL+RK VLLLISDLNI  DELSI +Q+
Sbjct: 334 HIDNMKILTALVHPKPHITPLQDGLTKRKVHLDVLRRKTVLLLISDLNILQDELSIFEQI 393

Query: 404 YNESRAQGMRVESQ----FEVVWIPIVD--HSIKWNDSMQKRFEYLLSIMPWHIVHHPTL 463
           Y ESR   + V+ +    +EVVW+P+VD     + +  +QK+FE L   MPW+ V  P L
Sbjct: 394 YTESRRNLVGVDGKSHMPYEVVWVPVVDPIEDFERSPILQKKFEDLRDPMPWYSVDSPKL 453

Query: 464 ISKAVTRFIGEVWQFRNKPILVVLDPQGKVVSPNAIHMMWIWGSLAFPFTSVKEEVLWRE 523
           I + V  F+   W F NKPILVV+DPQG   S NA+HM+WIWG+ AFPFT  +EE LWR 
Sbjct: 454 IERHVVEFMRGRWHFMNKPILVVIDPQGNEASLNALHMIWIWGTEAFPFTRSREEELWRR 513

Query: 524 ETWRLELLVDGIDPAVLNWIKEERYVFLYGGDDIEWIRKFTTTAKTVAQAARIPLEMVYV 583
           ET+ L L+VDGID  + NWIK + Y+FLYGGDD++WIR+FT  AK  A+ + + LEM YV
Sbjct: 514 ETFSLNLIVDGIDSVIFNWIKPDNYIFLYGGDDLDWIRRFTMAAKATAKDSNVNLEMAYV 573

Query: 584 GK--SSKRERVKKIITTITTEKLGYCWQDLTMIWFFWTRIESMLFSKIQLGKADDCDPLM 643
           GK   S RE++++I   I +E L + W +  ++WFFWTR+ESML+SKIQLGKADD D +M
Sbjct: 574 GKRNHSHREQIRRISEVIRSENLSHSWAEPALMWFFWTRLESMLYSKIQLGKADDHDDVM 633

Query: 644 QEIKKLLSYDKEGGWAVLSKGSTVILNGHSTTVLPTLGSYD-SWKQQAAEKGFDIAFKNH 703
           Q IKK+LSYDK GGWA+LSKG  +++  H   +  T+  YD +WK     KG+  A  +H
Sbjct: 634 QGIKKILSYDKLGGWALLSKGPEIVMIAHG-AIERTMSVYDRTWKTHVPTKGYTKAMSDH 693

Query: 704 HDE--LQGITHPCCRFEFPHT--TGRIPENFKCPECDRQMEKLTTFLCCHDENSDE 747
           H +  L+    PC  F+F  T  +GRIPE   C EC R MEK  +F CCHDE   E
Sbjct: 694 HHDEVLRETGKPCGHFDFHITARSGRIPEKMNCFECQRPMEKYMSFSCCHDEKLHE 734

BLAST of Lsi05G004670 vs. Swiss-Prot
Match: SEOA_ARATH (Protein SIEVE ELEMENT OCCLUSION A OS=Arabidopsis thaliana GN=SEOA PE=1 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 3.1e-120
Identity = 265/738 (35.91%), Postives = 398/738 (53.93%), Query Frame = 1

Query: 28  KFSLASRHQLAKGNKFLSAISDDNVMMKQIIATHDPDDRDVDTRSLLRLVENILKRATLA 87
           +F    +    +  + + ++SDD VM  +++ TH PD    D  SLL +V +I K    +
Sbjct: 117 RFGPGKKQAFHRNGRPMFSLSDDRVMADRVLKTHSPDMIFFDVTSLLSVVNDIFKSHVPS 176

Query: 88  ADATVWNESLPTKQGSYEQLESLEETGTHQAGFTTMLETLSYTIDRISSEISYKALEGID 147
            D++    SL  K           +   H     T  ET +  ID+IS EI  K L G +
Sbjct: 177 IDSSAPKPSLVFK-----------DYADH-----TSFETFADLIDQISCEIDCKCLHGGE 236

Query: 148 PHA-------------TTLAIFNMLASYRWDAKLVLTLAAFALNYGEFWLLAQIYSENQL 207
            H              TT ++ ++++ YRWDAKLVL L+A A+ YG F LLA+ ++ NQL
Sbjct: 237 SHGMMTSGLHLDSRNTTTFSVLSLVSKYRWDAKLVLVLSALAVKYGVFLLLAETHATNQL 296

Query: 208 AKAMAILKQLPGIFEHSIALKPKFDALKDLVAAILDVTWCIIDLKELPSAYISQEVPAMS 267
            K++A++KQLP IF    AL  + D  + L+  ++D+T  IID+ +LP  +I+      +
Sbjct: 297 TKSLALIKQLPSIFSRQNALHQRLDKTRILMQDMVDLTTTIIDIYQLPPNHIT------A 356

Query: 268 TAVAHIPTAVYWTIRSIVSSATQITSLTSMGYELALSTSTDSWELSTLAHKLKNIRDHLK 327
               HIPTAVYW +R ++   + I+  +    +  +S    S E+   + +L+ I  +L 
Sbjct: 357 AFTDHIPTAVYWIVRCVLICVSHISGASGFKQDQIMSFMEVS-EIHENSERLRKINAYLL 416

Query: 328 KKLVLCHQYIEEKRDIESFQMLINLLE-MTHLDNMKVLKALIYAKDDLQSLVDGSTGQRV 387
           ++       IEE    E +Q LI     + H+D +  L  L+   D L     G + +RV
Sbjct: 417 EQFKKSKMTIEEGIIEEEYQELIQTFTTIIHVDVVPPLLRLLRPIDFLYHGA-GVSKRRV 476

Query: 388 NLDVLKRKNVLLLISDLNISHDELSILDQLYNESRAQGMRVESQFEVVWIPIVDHSIKWN 447
            ++VL +K+VLLLISDL     EL IL+ LY E+  Q       FE++W+P+ D    W 
Sbjct: 477 GINVLTQKHVLLLISDLENIEKELYILESLYTEAWQQS------FEILWVPVQDF---WT 536

Query: 448 DSMQKRFEYLLSIMPWHIVHHPTLISKAVTRFIGEVWQFRNKPILVVLDPQGKVVSPNAI 507
           ++   +FE L   M W+++  P  + +A  RF+ E W F+N+PILV LDP+G+V+S NA 
Sbjct: 537 EADDAKFEALHMNMRWYVLGEPRKLRRAAIRFVREWWGFKNRPILVALDPKGQVMSTNAF 596

Query: 508 HMMWIWGSLAFPFTSVKEEVLWREETWRLELLVDGIDPAVLNWIKEERYVFLYGGDDIEW 567
            M+WIW   A PFT+ +E  LW E+ W LE L+DG DP  LN + + +Y+ LYGG+D++W
Sbjct: 597 PMVWIWQPFAHPFTTARERDLWSEQEWNLEFLIDGTDPHSLNQLVDGKYICLYGGEDMQW 656

Query: 568 IRKFTTTAKTVAQAARIPLEMVYVGKSSKRERVKKIITTITTEKLGYCWQDLTMIWFFWT 627
           I+ FT+  + VA+AA I LEMVYVGK + +  ++ II TI  E L +   DL  IWFFWT
Sbjct: 657 IKNFTSLWRNVAKAANIQLEMVYVGKRNPKNGIQPIINTIREENLSHTLPDLFQIWFFWT 716

Query: 628 RIESMLFSKIQLGKA-----------DDCDPLMQEIKKLLSYDKEG-GWAVLSKGSTVIL 687
           R+ESM  SK ++ KA           ++ D ++QE+  +L Y  EG GW ++SK S +++
Sbjct: 717 RVESMWESKQRMLKAHGIKGREGFKEEEKDLVLQEVVAMLGYGGEGDGWGLVSKASDMMV 776

Query: 688 NGHSTTVLPTLGSYDSWKQQAAEKGFDIAFKNHHDELQGITHPCCRFEFPHTTGRIPENF 740
                     L  ++ W+     KGF  A  N H  ++   H C RF  P T G IP   
Sbjct: 777 RAKGNLFSRGLAEFNEWEVNIPTKGFLTAL-NDHLLMRLPPHHCTRFMLPETAGIIPNEV 820

BLAST of Lsi05G004670 vs. Swiss-Prot
Match: SEOC_ARATH (Protein SIEVE ELEMENT OCCLUSION C OS=Arabidopsis thaliana GN=SEOC PE=4 SV=2)

HSP 1 Score: 246.5 bits (628), Expect = 9.1e-64
Identity = 195/708 (27.54%), Postives = 337/708 (47.60%), Query Frame = 1

Query: 44  LSAISDDNVMMKQIIATHDPDDRDVDTRSLLRLVENILKRATLAADATVWNESLPTKQGS 103
           +SA+++D ++++Q++ +HDPD R +D+  LL+ VE IL    L  D      S P     
Sbjct: 7   ISALNED-IIVEQLLRSHDPDGRWLDSEMLLQEVETILS-FVLQNDV-----SRPL---- 66

Query: 104 YEQLESLEETGTHQAGFTTMLETLSYTIDRISSEISYKALEGIDPHATTLAIFNMLASYR 163
                 L E            ETL Y I RIS ++        +    T+ +F++L  YR
Sbjct: 67  ------LTENCITTIEVFDSKETLPYAIFRISVQMLCPCTGENEIRKRTMVLFDLLKEYR 126

Query: 164 WDAKLVLTLAAFALNYGEFWLLAQIYSENQLAKAMAILKQLPGIFEHSIALKPKFDALKD 223
           WDAK VL L   A  YG   L   +   + +A ++A L QLP         +P  ++L  
Sbjct: 127 WDAKAVLVLGVLAATYGGLLLPVHLAICDPVAASIAKLNQLP---IERTKFRPWLESLNL 186

Query: 224 LVAAILDVTWCIIDLKELPSAYISQEVPAMSTAVAHIPTAVYWTIRSIVSSATQI----- 283
           L+ A++DVT CII  +++P      +   +   +++I    Y  ++S ++   QI     
Sbjct: 187 LIKAMVDVTKCIIKFEKIPFKQAKLDNNILGETLSNIYLTTYRVVKSALTCMQQIPYFKQ 246

Query: 284 ----TSLTSMGYELALSTSTDSWELSTLAHKLKNIRDHLKKKLVLCHQYIEEKRDIESFQ 343
                       EL++ +   + ELS+L ++L NI   L K++  C   IEE    E  Q
Sbjct: 247 TQQAKKSRKTAAELSIESRRAAGELSSLGYQLLNIHTRLNKQVEDCSTQIEE----EINQ 306

Query: 344 MLINLLEMTHLDNMKVLKALIYAKDDLQSLVDGSTGQRVNLDVLKRKNVLLLISDLNISH 403
            L N+   TH DN  VL  L   +DDL         +++++  ++ K  LLL+S   +  
Sbjct: 307 RLRNINIETHQDNQDVLHLLFSLQDDLPL---QQYSRQISITEVQDKVTLLLLSKPPV-E 366

Query: 404 DELSILDQLYNESRAQGMRVESQFEVVWIPIVDHSIKWNDSMQKRFEYLLSIMPWHIVHH 463
               +L QLY+         E  +E++W+PI   S KW D  ++ F++  + +PW  V  
Sbjct: 367 PLFFLLQQLYD--HPSNTNTEQNYEIIWVPI-PSSQKWTDEEKEIFDFYSNSLPWISVRQ 426

Query: 464 PTLISKAVTRFIGEVWQFR-NKPILVVLDPQGKVVSPNAIHMMWIWGSLAFPFTSVKEEV 523
           P L+S  +  F  + W ++ N+ +LVV+D  G+ V+ NA+ M+ IWG  A+PF+  +E+ 
Sbjct: 427 PWLMSSTILNFFKQEWHYKDNEAMLVVIDSNGRFVNMNAMDMVLIWGVKAYPFSVSREDE 486

Query: 524 LWREETWRLELLVDGIDPAVLNWIKEERYVFLYGGDDIEWIRKFTTTAKTVAQAARIPLE 583
           LW+E  W + LL+DGI P       E R + ++G ++++WI +F + A+ + Q     LE
Sbjct: 487 LWKEHGWSINLLLDGIHPTF-----EGREICIFGSENLDWIDEFVSLARKI-QNLGFQLE 546

Query: 584 MVYVGKSSKRERVKKIITTITTEKLGYCWQDLTMIWFFWTRIESMLFSKIQ--LGKADDC 643
           ++Y+    + ER  +  + + +          T+   FW R+ES+  SK++  + +    
Sbjct: 547 LIYLSNQRRDERAMEESSILFSP---------TLQQLFWLRLESIERSKLKRIVIEPSKP 606

Query: 644 DPLMQEIKKLLSYD--KEGGWAVLSKGSTV-ILNGHSTTVLPTLGSYDSWKQQAAEKGFD 703
           D + +E++ LL +D  K  GW ++  GST   ++G   T    +     W + A   GF 
Sbjct: 607 DRVFEEVRNLLDFDYGKHRGWGIIGNGSTAETVDGEKMT--ERMRKIVRWGEYAKGLGFT 663

Query: 704 IAFKNHHDELQGITHPCCRFEFPHTTGRIPENFKCPECDRQMEKLTTF 737
            A +   ++   ++H       P       +   C +C   M++   +
Sbjct: 667 EAIEIAAEKPCELSHTAV---VPFEEALTMKVVTCEKCKWPMKRFVAY 663

BLAST of Lsi05G004670 vs. TrEMBL
Match: A0A0A0LBZ8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G597260 PE=4 SV=1)

HSP 1 Score: 1377.8 bits (3565), Expect = 0.0e+00
Identity = 686/724 (94.75%), Postives = 702/724 (96.96%), Query Frame = 1

Query: 23  MDGSNKFSLASRHQLAKGNKFLSAISDDNVMMKQIIATHDPDDRDVDTRSLLRLVENILK 82
           MD  NKFSLASRHQLAKGNKFLSAISDDNVMMKQI+ATHDPDDRDVDTRSLLRLVENILK
Sbjct: 1   MDSGNKFSLASRHQLAKGNKFLSAISDDNVMMKQILATHDPDDRDVDTRSLLRLVENILK 60

Query: 83  RATLAADATVWNESLPTKQGSYEQLESLEETGTHQAGFTTMLETLSYTIDRISSEISYKA 142
           RATLAADAT          GSYEQLESLEETGTHQAG TTMLE LSYTIDRISSEISYKA
Sbjct: 61  RATLAADAT----------GSYEQLESLEETGTHQAGLTTMLEALSYTIDRISSEISYKA 120

Query: 143 LEGIDPHATTLAIFNMLASYRWDAKLVLTLAAFALNYGEFWLLAQIYSENQLAKAMAILK 202
           LEGIDPHATTLAIFNMLASYRWDAKLVLTLAAFALNYGEFWLLAQIYS+NQLAKAMAILK
Sbjct: 121 LEGIDPHATTLAIFNMLASYRWDAKLVLTLAAFALNYGEFWLLAQIYSQNQLAKAMAILK 180

Query: 203 QLPGIFEHSIALKPKFDALKDLVAAILDVTWCIIDLKELPSAYISQEVPAMSTAVAHIPT 262
           QLPGIFEHSIALKPKFDALK+LVAAILDVTWCIIDLKELPSAYISQEVPAMSTAVAHIPT
Sbjct: 181 QLPGIFEHSIALKPKFDALKELVAAILDVTWCIIDLKELPSAYISQEVPAMSTAVAHIPT 240

Query: 263 AVYWTIRSIVSSATQITSLTSMGYELALSTSTDSWELSTLAHKLKNIRDHLKKKLVLCHQ 322
           AVYWTIRSIVSSATQITSLTSMGYELALSTSTD+WELSTLAHKLKNI DHLKKKLVLCHQ
Sbjct: 241 AVYWTIRSIVSSATQITSLTSMGYELALSTSTDAWELSTLAHKLKNICDHLKKKLVLCHQ 300

Query: 323 YIEEKRDIESFQMLINLLEMTHLDNMKVLKALIYAKDDLQSLVDGSTGQRVNLDVLKRKN 382
           +IEEK+DIESFQMLINL EM HLDNMKVLKALIY KDDLQ LVDGSTGQRVNLDVLKRKN
Sbjct: 301 HIEEKKDIESFQMLINLFEMNHLDNMKVLKALIYPKDDLQPLVDGSTGQRVNLDVLKRKN 360

Query: 383 VLLLISDLNISHDELSILDQLYNESRAQGMRVESQFEVVWIPIVDHSIKWNDSMQKRFEY 442
           VLLLISDLNISHDELSILDQLYNESRAQGMRVESQFEVVWIPIVDHSIKWNDSMQKRFEY
Sbjct: 361 VLLLISDLNISHDELSILDQLYNESRAQGMRVESQFEVVWIPIVDHSIKWNDSMQKRFEY 420

Query: 443 LLSIMPWHIVHHPTLISKAVTRFIGEVWQFRNKPILVVLDPQGKVVSPNAIHMMWIWGSL 502
           LLSIMPWHIVHHPTLISKAVTRFIGEVWQFRN+PILVVLDPQGKVVSPNAIHMMWIWGSL
Sbjct: 421 LLSIMPWHIVHHPTLISKAVTRFIGEVWQFRNRPILVVLDPQGKVVSPNAIHMMWIWGSL 480

Query: 503 AFPFTSVKEEVLWREETWRLELLVDGIDPAVLNWIKEERYVFLYGGDDIEWIRKFTTTAK 562
           AFPFTSVKEEVLW+EETWRLELLVDGIDPAVLNWIKEERY+FLYGGDDIEWIRKFTTTAK
Sbjct: 481 AFPFTSVKEEVLWKEETWRLELLVDGIDPAVLNWIKEERYIFLYGGDDIEWIRKFTTTAK 540

Query: 563 TVAQAARIPLEMVYVGKSSKRERVKKIITTITTEKLGYCWQDLTMIWFFWTRIESMLFSK 622
           TVAQAARIPLEMVYVGKSSKRERVKKIITTITTEKLGYCWQDLTMIWFFWTRIESML+SK
Sbjct: 541 TVAQAARIPLEMVYVGKSSKRERVKKIITTITTEKLGYCWQDLTMIWFFWTRIESMLYSK 600

Query: 623 IQLGKADDCDPLMQEIKKLLSYDKEGGWAVLSKGSTVILNGHSTTVLPTLGSYDSWKQQA 682
           IQLGKADDCDPLMQEIKKLLSYDKEGGWAVLSKGS VILNGHSTT+LPTLGS+DSWKQ+A
Sbjct: 601 IQLGKADDCDPLMQEIKKLLSYDKEGGWAVLSKGSNVILNGHSTTMLPTLGSFDSWKQEA 660

Query: 683 AEKGFDIAFKNHHDELQGITHPCCRFEFPHTTGRIPENFKCPECDRQMEKLTTFLCCHDE 742
            +KGFDIAFKNHHDELQGITHPCCRFEFPHT+GRIPENFKCP CDRQMEKLTTFLCCHDE
Sbjct: 661 TDKGFDIAFKNHHDELQGITHPCCRFEFPHTSGRIPENFKCPGCDRQMEKLTTFLCCHDE 714

Query: 743 NSDE 747
           NS+E
Sbjct: 721 NSNE 714

BLAST of Lsi05G004670 vs. TrEMBL
Match: A0A067LGB3_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_10213 PE=4 SV=1)

HSP 1 Score: 1008.1 bits (2605), Expect = 5.8e-291
Identity = 480/711 (67.51%), Postives = 590/711 (82.98%), Query Frame = 1

Query: 36  QLAKGNKFLSAISDDNVMMKQIIATHDPDDRDVDTRSLLRLVENILKRATLAADATVWNE 95
           QL KG++ + A+SD+N M+KQI ATH PD R+ D R LL +V++IL RATL  D+ +   
Sbjct: 18  QLIKGDRSMLALSDENSMLKQIQATHAPDGREFDVRPLLNIVDDILSRATLHGDSALM-- 77

Query: 96  SLPTKQGSYEQLESLEETGTHQAGFTTMLETLSYTIDRISSEISYKALEGIDPHATTLAI 155
                     Q  +  +    QA F  MLE LS+ IDRI+SEISYKAL G D HATTL++
Sbjct: 78  --------VNQTHAEVDDKNRQANFIAMLEALSFVIDRIASEISYKALSGADAHATTLSL 137

Query: 156 FNMLASYRWDAKLVLTLAAFALNYGEFWLLAQIYSENQLAKAMAILKQLPGIFEHSIALK 215
           FNML+SY WDAK+VLT+AAFALNYGEFWLLAQIYS N LAK+MAILKQLP I EH+  LK
Sbjct: 138 FNMLSSYSWDAKVVLTMAAFALNYGEFWLLAQIYSSNPLAKSMAILKQLPVILEHTGPLK 197

Query: 216 PKFDALKDLVAAILDVTWCIIDLKELPSAYISQEVPAMSTAVAHIPTAVYWTIRSIVSSA 275
           P+FDAL +L++ ++DVT CI++ KELPS+YIS EVPA+STA+AHIPTAVYWT+RS+V+ A
Sbjct: 198 PRFDALNNLISVMMDVTKCIVEFKELPSSYISSEVPALSTAIAHIPTAVYWTMRSVVACA 257

Query: 276 TQITSLTSMGYELALSTSTDSWELSTLAHKLKNIRDHLKKKLVLCHQYIEEKRDIESFQM 335
           TQIT+LT+MG+E A   +T++WELSTLAHKL+NI +HL+K+L +C+Q+IEEKR++E++Q 
Sbjct: 258 TQITNLTTMGHEFA---TTEAWELSTLAHKLQNILEHLRKQLSICYQHIEEKRNVEAYQA 317

Query: 336 LINLLEMTHLDNMKVLKALIYAKDDLQSLVDGSTGQRVNLDVLKRKNVLLLISDLNISHD 395
           L+NL +  H+DNMK+LKALIYAKDD+Q LVDGST +RVN+DVL+RKNVLLLIS L+ISHD
Sbjct: 318 LLNLFDTIHIDNMKILKALIYAKDDIQPLVDGSTKRRVNVDVLRRKNVLLLISGLDISHD 377

Query: 396 ELSILDQLYNESRAQGMRVESQFEVVWIPIVDHSIKWNDSMQKRFEYLLSIMPWHIVHHP 455
           ELSIL+Q+YNESR    R+ESQ+EVVW+P+VD ++ + D+M K+FE L ++MPW+ V+HP
Sbjct: 378 ELSILEQIYNESRIHATRLESQYEVVWVPVVDRTVPYTDAMHKQFESLQTVMPWYTVYHP 437

Query: 456 TLISKAVTRFIGEVWQFRNKPILVVLDPQGKVVSPNAIHMMWIWGSLAFPFTSVKEEVLW 515
           TLI K V RFI EVW FR +PILVVLDPQGKVV PNA+HMMWIW S AFPFTS +EE LW
Sbjct: 438 TLIEKVVIRFIKEVWHFRRRPILVVLDPQGKVVCPNALHMMWIWASNAFPFTSYREESLW 497

Query: 516 REETWRLELLVDGIDPAVLNWIKEERYVFLYGGDDIEWIRKFTTTAKTVAQAARIPLEMV 575
           +EETWRLELL+DGIDP +LNWIKE +Y+FLYGGDD+EWIRKFT TA+ VAQAARI LEMV
Sbjct: 498 KEETWRLELLIDGIDPTILNWIKEGKYIFLYGGDDVEWIRKFTNTARAVAQAARISLEMV 557

Query: 576 YVGKSSKRERVKKIITTITTEKLGYCWQDLTMIWFFWTRIESMLFSKIQLGKADDCDPLM 635
           YVGKS KR++V++II+TIT EKL Y WQDLTMIWFFWTR++SML+SKIQLGK D+ DP+M
Sbjct: 558 YVGKSCKRDKVQRIISTITVEKLSYVWQDLTMIWFFWTRLQSMLYSKIQLGKIDEHDPMM 617

Query: 636 QEIKKLLSYDKEGGWAVLSKGSTVILNGHSTTVLPTLGSYDSWKQQAAEKGFDIAFKNHH 695
           QEIKKLLSYDKEGGWAVLS+GS +++NGHSTTVLPTL  YD WK+  + KGFD++FK HH
Sbjct: 618 QEIKKLLSYDKEGGWAVLSRGSNIVVNGHSTTVLPTLLEYDIWKENVSLKGFDLSFKEHH 677

Query: 696 DELQGITHPCCRFEFPHTTGRIPENFKCPECDRQMEKLTTFLCCHDENSDE 747
           D+L GI HPCCRFEFP TTGRIPE  KCPEC+R MEK  TFLCCH+E + E
Sbjct: 678 DKLHGIAHPCCRFEFPSTTGRIPERLKCPECNRNMEKHITFLCCHEEETSE 715

BLAST of Lsi05G004670 vs. TrEMBL
Match: B9IJL5_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0017s10720g PE=4 SV=2)

HSP 1 Score: 1005.4 bits (2598), Expect = 3.7e-290
Identity = 482/706 (68.27%), Postives = 582/706 (82.44%), Query Frame = 1

Query: 36  QLAKGNKFLSAISDDNVMMKQIIATHDPDDRDVDTRSLLRLVENILKRATLAADATVWNE 95
           QL + ++ +  +SDDNVMMK+I+ TH PD R+VD + LL LVE+ILKRATL  D      
Sbjct: 20  QLIRSDRSMITMSDDNVMMKRIVETHAPDGREVDVKPLLHLVEDILKRATLQTDT----- 79

Query: 96  SLPTKQGSYEQLESLEETGTHQAGFTTMLETLSYTIDRISSEISYKALEGIDPHATTLAI 155
           SL T Q   E      E  T+ A F  ML++LSYTIDRIS EI+YK   G D HATT+ +
Sbjct: 80  SLTTSQAHAES-----EDKTNHASFAVMLDSLSYTIDRISCEIAYKG--GADGHATTVEL 139

Query: 156 FNMLASYRWDAKLVLTLAAFALNYGEFWLLAQIYSENQLAKAMAILKQLPGIFEHSIALK 215
           FNMLASY WDAKLVLTLAAFALNYGEFWLLAQIYS NQLAK+MAILKQLP I EHS  LK
Sbjct: 140 FNMLASYSWDAKLVLTLAAFALNYGEFWLLAQIYSSNQLAKSMAILKQLPNILEHSGPLK 199

Query: 216 PKFDALKDLVAAILDVTWCIIDLKELPSAYISQEVPAMSTAVAHIPTAVYWTIRSIVSSA 275
           P+FDAL +L+  ++DVT C+++ K+LP  YIS EV A+S A+AH+PTAVYWT+RS+++ A
Sbjct: 200 PRFDALNNLIKVMMDVTRCVVEFKDLPPTYISHEVSALSAAMAHVPTAVYWTVRSVLACA 259

Query: 276 TQITSLTSMGYELALSTSTDSWELSTLAHKLKNIRDHLKKKLVLCHQYIEEKRDIESFQM 335
            QITSLT+MGYE ++ST T +WELSTLAHKL NI +HL+++L  C+QYI+EKR++E++QM
Sbjct: 260 AQITSLTTMGYEFSIST-TKAWELSTLAHKLSNILEHLRRQLATCYQYIDEKRNVEAYQM 319

Query: 336 LINLLEMTHLDNMKVLKALIYAKDDLQSLVDGSTGQRVNLDVLKRKNVLLLISDLNISHD 395
           L+NL EM H+DNMKVLKALIYAKDD+Q L+DGS  +RV+LDVL+RKNVLLLIS L+IS+D
Sbjct: 320 LLNLFEMIHIDNMKVLKALIYAKDDIQPLIDGSNKRRVHLDVLRRKNVLLLISGLDISND 379

Query: 396 ELSILDQLYNESRAQGMRVESQFEVVWIPIVDHSIKWNDSMQKRFEYLLSIMPWHIVHHP 455
           EL+IL+Q+YNES   G R++SQ+++VWIPI DHS++W D ++++FE L + MPW+ V+HP
Sbjct: 380 ELAILEQIYNESSHHGTRLDSQYDLVWIPITDHSVQWTDPLKEKFESLQNSMPWYTVYHP 439

Query: 456 TLISKAVTRFIGEVWQFRNKPILVVLDPQGKVVSPNAIHMMWIWGSLAFPFTSVKEEVLW 515
           +LI KA  RFI EVW FRNKPILVVLDPQGKVVSPNA+HMMWIWGS AFPFTS++EE LW
Sbjct: 440 SLIDKAAIRFIREVWHFRNKPILVVLDPQGKVVSPNALHMMWIWGSNAFPFTSLREESLW 499

Query: 516 REETWRLELLVDGIDPAVLNWIKEERYVFLYGGDDIEWIRKFTTTAKTVAQAARIPLEMV 575
           REETWRLELLVDGIDP +LNWIKEE+Y+F+YGGDD+EW+RKFT TA+ VAQAARIPLEMV
Sbjct: 500 REETWRLELLVDGIDPVILNWIKEEKYIFMYGGDDVEWVRKFTNTARAVAQAARIPLEMV 559

Query: 576 YVGKSSKRERVKKIITTITTEKLGYCWQDLTMIWFFWTRIESMLFSKIQLGKADDCDPLM 635
           YVGKS KRE++++++ TI  EKL Y WQDLTMIWFFWTR+ESMLFSKIQLGK DD DP+M
Sbjct: 560 YVGKSRKREQIRRVMGTINVEKLSYAWQDLTMIWFFWTRLESMLFSKIQLGKVDDHDPMM 619

Query: 636 QEIKKLLSYDKEGGWAVLSKGSTVILNGHSTTVLPTLGSYDSWKQQAAEKGFDIAFKNHH 695
           Q IKKLLSYD+EGGWAVLSKGS+V++NGH TTVLPTL  YD WK+Q   KGFD+AF+ HH
Sbjct: 620 QAIKKLLSYDREGGWAVLSKGSSVVVNGHGTTVLPTLVEYDLWKEQVPVKGFDLAFQEHH 679

Query: 696 DELQGITHPCCRFEFPHTTGRIPENFKCPECDRQMEKLTTFLCCHD 742
             L  I HPC RFEFP T GRIPE  KCPEC+R MEK TTFLCCHD
Sbjct: 680 GNLHDIVHPCSRFEFPMTAGRIPETLKCPECNRSMEKFTTFLCCHD 712

BLAST of Lsi05G004670 vs. TrEMBL
Match: U5GX92_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0001s35900g PE=4 SV=1)

HSP 1 Score: 1000.3 bits (2585), Expect = 1.2e-288
Identity = 485/711 (68.21%), Postives = 584/711 (82.14%), Query Frame = 1

Query: 32  ASRHQLAKGNKFLSAISDDNVMMKQIIATHDPDDRDVDTRSLLRLVENILKRATLAADAT 91
           AS+  +      +  +SDDNVMMKQI+ TH PD R+VD + LL LVE+ILKRATL  D+ 
Sbjct: 15  ASQQLIKSDRGSMLTMSDDNVMMKQIVGTHAPDGREVDVKPLLLLVEDILKRATLQIDS- 74

Query: 92  VWNESLPTKQGSYEQLESLEETGTHQAGFTTMLETLSYTIDRISSEISYKALEGIDPHAT 151
               SL T Q   E      E  T    F +ML+ LSYTIDRISSEI+YKAL G D HAT
Sbjct: 75  ----SLTTSQAHAEM-----EDKTSHVNFVSMLDALSYTIDRISSEIAYKALGGTDAHAT 134

Query: 152 TLAIFNMLASYRWDAKLVLTLAAFALNYGEFWLLAQIYSENQLAKAMAILKQLPGIFEHS 211
           T+++FNML SY WDAKLVLTL+AFALNYGEFWLLAQIYS N LAK+MAIL+QLP I EHS
Sbjct: 135 TVSLFNMLTSYSWDAKLVLTLSAFALNYGEFWLLAQIYSSNDLAKSMAILRQLPSIMEHS 194

Query: 212 IALKPKFDALKDLVAAILDVTWCIIDLKELPSAYISQEVPAMSTAVAHIPTAVYWTIRSI 271
             LKP+FDA+ +L+  ++DV  C+++ K+LP AYIS EVPA+STA+AHIPTAVYWT+RS+
Sbjct: 195 GPLKPRFDAINNLIKVMMDVARCMVEFKDLPPAYISNEVPALSTAMAHIPTAVYWTMRSV 254

Query: 272 VSSATQITSLTSMGYELALSTSTDSWELSTLAHKLKNIRDHLKKKLVLCHQYIEEKRDIE 331
           V+ A QITSLT+MG+E ++ST TD+WELSTLAHKL NI DHL+K+L  C+QYI+EKR++E
Sbjct: 255 VACAAQITSLTTMGHEFSIST-TDAWELSTLAHKLSNILDHLRKQLDTCYQYIDEKRNVE 314

Query: 332 SFQMLINLLEMTHLDNMKVLKALIYAKDDLQSLVDGSTGQRVNLDVLKRKNVLLLISDLN 391
           SFQML NL EM H+DNMKVLKALIYAKDD+Q L+DGS+ +RV+LDVL+RKNVLLLIS L+
Sbjct: 315 SFQMLKNLFEMIHIDNMKVLKALIYAKDDIQPLIDGSSKKRVHLDVLRRKNVLLLISGLD 374

Query: 392 ISHDELSILDQLYNESRAQGMRVESQFEVVWIPIVDHSIKWNDSMQKRFEYLLSIMPWHI 451
           +  DELSIL+Q+YNESR  G R+++Q+EVVW+PIVD S++W+D M+ +FE + S MPW  
Sbjct: 375 MPTDELSILEQIYNESRHHGPRLDNQYEVVWVPIVDRSVQWSDPMKGKFESMQSSMPWFT 434

Query: 452 VHHPTLISKAVTRFIGEVWQFRNKPILVVLDPQGKVVSPNAIHMMWIWGSLAFPFTSVKE 511
           V+HP+LI KAV RFI EVW FRNKPILVVLDPQGKVV PNA+HMMWIWGS AFPFTS++E
Sbjct: 435 VYHPSLIEKAVIRFIKEVWHFRNKPILVVLDPQGKVVCPNALHMMWIWGSNAFPFTSLRE 494

Query: 512 EVLWREETWRLELLVDGIDPAVLNWIKEERYVFLYGGDDIEWIRKFTTTAKTVAQAARIP 571
           E LW++ETWRLELLVDGIDP +LNWI E +Y+FLYGGDD EW+RKFT TA+ VAQAARIP
Sbjct: 495 ESLWKDETWRLELLVDGIDPVILNWINEGKYIFLYGGDDDEWVRKFTNTARAVAQAARIP 554

Query: 572 LEMVYVGKSSKRERVKKIITTITTEKLGYCWQDLTMIWFFWTRIESMLFSKIQLGKADDC 631
           LEMVYVGKSSKRE+++++I TIT EKL Y WQDLTM+WFFWTR+ESML+SKIQLGK DD 
Sbjct: 555 LEMVYVGKSSKREKIRRVIATITVEKLSYVWQDLTMMWFFWTRLESMLYSKIQLGKLDDH 614

Query: 632 DPLMQEIKKLLSYDKEGGWAVLSKGSTVILNGHSTTVLPTLGSYDSWKQQAAEKGFDIAF 691
           DP+MQEIKKLLSY +EGGWAVLS GS V++NGH TTVL TL  YD WK+Q   KGFD+AF
Sbjct: 615 DPMMQEIKKLLSYGREGGWAVLSNGSNVVVNGHKTTVLQTLLEYDLWKEQVPVKGFDMAF 674

Query: 692 KNHHDELQGITHPCCRFEFPHTTGRIPENFKCPECDRQMEKLTTFLCCHDE 743
           ++HH +L+GI  PCCRF+FP TTGRIPE  KCPEC+  MEK +TFLCCHDE
Sbjct: 675 QDHHFQLRGIARPCCRFDFPMTTGRIPETMKCPECNSTMEKFSTFLCCHDE 714

BLAST of Lsi05G004670 vs. TrEMBL
Match: B9GIM3_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0001s35870g PE=4 SV=2)

HSP 1 Score: 999.6 bits (2583), Expect = 2.0e-288
Identity = 484/711 (68.07%), Postives = 581/711 (81.72%), Query Frame = 1

Query: 32  ASRHQLAKGNKFLSAISDDNVMMKQIIATHDPDDRDVDTRSLLRLVENILKRATLAADAT 91
           AS+  +      +  +SDDNVMMKQI+ TH PD R+VD + LL LVE+ILKRAT   D  
Sbjct: 16  ASQQLIKSDRGSMLTMSDDNVMMKQIVGTHAPDGREVDVKPLLHLVEDILKRATQQIDT- 75

Query: 92  VWNESLPTKQGSYEQLESLEETGTHQAGFTTMLETLSYTIDRISSEISYKALEGIDPHAT 151
               SL T Q   E      E  THQ  F +ML+ LSYTIDRIS EI+YKAL G D HAT
Sbjct: 76  ----SLTTSQAHAEL-----EDKTHQVNFVSMLDALSYTIDRISCEIAYKALGGTDAHAT 135

Query: 152 TLAIFNMLASYRWDAKLVLTLAAFALNYGEFWLLAQIYSENQLAKAMAILKQLPGIFEHS 211
           T+++FNML SY WDAKLVLTLAAFALNYGEFWLLAQIYS N LAK+MAIL+QLP I EHS
Sbjct: 136 TVSLFNMLTSYSWDAKLVLTLAAFALNYGEFWLLAQIYSSNDLAKSMAILRQLPSIMEHS 195

Query: 212 IALKPKFDALKDLVAAILDVTWCIIDLKELPSAYISQEVPAMSTAVAHIPTAVYWTIRSI 271
             LKP+FDA+ +L+  ++DV  C+++ K+LP AYIS EVPA+STA+AHIPTAVYWT+RS+
Sbjct: 196 GPLKPRFDAINNLIKVMMDVARCVVEFKDLPPAYISNEVPALSTAMAHIPTAVYWTMRSV 255

Query: 272 VSSATQITSLTSMGYELALSTSTDSWELSTLAHKLKNIRDHLKKKLVLCHQYIEEKRDIE 331
           V+ A QITSLT+MG+E ++ST T +WELSTLAHKL NI DHL+K+L  C+QYI+EKR++E
Sbjct: 256 VACAAQITSLTTMGHEFSIST-TMAWELSTLAHKLSNILDHLRKQLDTCYQYIDEKRNVE 315

Query: 332 SFQMLINLLEMTHLDNMKVLKALIYAKDDLQSLVDGSTGQRVNLDVLKRKNVLLLISDLN 391
           SFQML NL EM H+DNMKVLKALIYAKDD+Q L+DGS+ +RV+LDVL+RKNVLLLIS L+
Sbjct: 316 SFQMLKNLFEMIHIDNMKVLKALIYAKDDIQPLIDGSSKKRVHLDVLRRKNVLLLISGLD 375

Query: 392 ISHDELSILDQLYNESRAQGMRVESQFEVVWIPIVDHSIKWNDSMQKRFEYLLSIMPWHI 451
           +S DELSIL+Q+YNESR  G R++SQ+EVVW+PIVD S++W+D M+ +FE + S MPW  
Sbjct: 376 MSTDELSILEQIYNESRQHGPRLDSQYEVVWVPIVDRSVQWSDPMKGKFESMQSSMPWFT 435

Query: 452 VHHPTLISKAVTRFIGEVWQFRNKPILVVLDPQGKVVSPNAIHMMWIWGSLAFPFTSVKE 511
           V+HP+LI KAV RFI EVW FRNKPILVVLDPQGKVV PNA+HMMWIWGS AFPFTS++E
Sbjct: 436 VYHPSLIEKAVIRFIKEVWHFRNKPILVVLDPQGKVVCPNALHMMWIWGSNAFPFTSLRE 495

Query: 512 EVLWREETWRLELLVDGIDPAVLNWIKEERYVFLYGGDDIEWIRKFTTTAKTVAQAARIP 571
           E LW++ETWRLELLVDGIDP +LNWIKE +Y+F+YGGDD EW+RKFT TA+ VAQAARIP
Sbjct: 496 ESLWKDETWRLELLVDGIDPVILNWIKEGKYIFMYGGDDDEWVRKFTNTARAVAQAARIP 555

Query: 572 LEMVYVGKSSKRERVKKIITTITTEKLGYCWQDLTMIWFFWTRIESMLFSKIQLGKADDC 631
           LEMVYVGKSSKRE+++++I TIT EKL Y WQDLTMIWFFWTR+ESML+SKIQLGK DD 
Sbjct: 556 LEMVYVGKSSKREKIRRVIATITVEKLSYVWQDLTMIWFFWTRLESMLYSKIQLGKLDDH 615

Query: 632 DPLMQEIKKLLSYDKEGGWAVLSKGSTVILNGHSTTVLPTLGSYDSWKQQAAEKGFDIAF 691
           DP+MQEIKKLLSYD+EGGWAVLS GS V++NGH TT L TL  YD WK+Q   KGFD+A+
Sbjct: 616 DPMMQEIKKLLSYDREGGWAVLSNGSNVVVNGHKTTALQTLLEYDLWKEQVPVKGFDLAY 675

Query: 692 KNHHDELQGITHPCCRFEFPHTTGRIPENFKCPECDRQMEKLTTFLCCHDE 743
           ++H   +  I+ PCCRF+FP T GRIPE  KCPEC+R MEK +TFLCCHDE
Sbjct: 676 RDHQGRIHDISRPCCRFDFPMTMGRIPETMKCPECNRTMEKFSTFLCCHDE 715

BLAST of Lsi05G004670 vs. TAIR10
Match: AT3G01680.1 (AT3G01680.1 Mediator complex subunit Med28 (InterPro:IPR021640))

HSP 1 Score: 675.6 bits (1742), Expect = 3.4e-194
Identity = 352/716 (49.16%), Postives = 485/716 (67.74%), Query Frame = 1

Query: 44  LSAISDDNVMMKQIIATHDPDDRDVDTRSLLRLVENILKRATLAADATVWNESLPTKQGS 103
           L+  SD+++M+K I  TH PD R+V  R LL LVE+IL RATL ++ T  N S+      
Sbjct: 34  LAMSSDESMMLKLIQQTHSPDAREVQVRGLLSLVEDILDRATLDSEDT--NASM------ 93

Query: 104 YEQLESLEETGTHQAGFTTMLETLSYTIDRISSEISYKALEGIDPHATTLAIFNMLASYR 163
              L    E    Q+   ++L+++SY IDR++ EI+YK+L G D H  T+++F  L+S++
Sbjct: 94  ---LPLPTEDKLMQSSMMSVLDSVSYAIDRVACEIAYKSLTGSDSHEITMSVFEHLSSFQ 153

Query: 164 WDAKLVLTLAAFALNYGEFWLLAQIYSENQLAKAMAILKQLPGIFEHSIALKPKFDALKD 223
           WD KLVLTLAAFALNYGEFWLL Q YS+NQLAK++A+LK +P   ++ + L+     L D
Sbjct: 154 WDGKLVLTLAAFALNYGEFWLLVQFYSKNQLAKSLAMLKLVP--VQNRVTLESVSQGLND 213

Query: 224 LVAAILDVTWCIIDLKELPSAYISQEVPAMSTAVAHIPTAVYWTIRSIVSSATQITSLTS 283
           L+  +  VT C+++L ELP  YI+ +VP +S  ++ IP AVYWTIRS+++  +QI  +T+
Sbjct: 214 LIREMKSVTACVVELSELPDRYITPDVPQLSRILSTIPIAVYWTIRSVIACISQINMITA 273

Query: 284 MGYELALSTSTDSWELSTLAHKLKNIRDHLKKKLVLCHQYIEEKRDIESFQMLINLLEMT 343
           MG+E+ ++T  D WE S LA+KLKNI DHL + L LC+++IE++R  ES ++L +L + T
Sbjct: 274 MGHEM-MNTQMDLWETSMLANKLKNIHDHLAETLRLCYRHIEKQRSSESLKVLHSLFDTT 333

Query: 344 HLDNMKVLKALIYAKDDLQSLVDGSTGQRVNLDVLKRKNVLLLISDLNISHDELSILDQL 403
           H+DNMK+L AL++ K  +  L DG T ++V+LDVL+RK VLLLISDLNI  DELSI +Q+
Sbjct: 334 HIDNMKILTALVHPKPHITPLQDGLTKRKVHLDVLRRKTVLLLISDLNILQDELSIFEQI 393

Query: 404 YNESRAQGMRVESQ----FEVVWIPIVD--HSIKWNDSMQKRFEYLLSIMPWHIVHHPTL 463
           Y ESR   + V+ +    +EVVW+P+VD     + +  +QK+FE L   MPW+ V  P L
Sbjct: 394 YTESRRNLVGVDGKSHMPYEVVWVPVVDPIEDFERSPILQKKFEDLRDPMPWYSVDSPKL 453

Query: 464 ISKAVTRFIGEVWQFRNKPILVVLDPQGKVVSPNAIHMMWIWGSLAFPFTSVKEEVLWRE 523
           I + V  F+   W F NKPILVV+DPQG   S NA+HM+WIWG+ AFPFT  +EE LWR 
Sbjct: 454 IERHVVEFMRGRWHFMNKPILVVIDPQGNEASLNALHMIWIWGTEAFPFTRSREEELWRR 513

Query: 524 ETWRLELLVDGIDPAVLNWIKEERYVFLYGGDDIEWIRKFTTTAKTVAQAARIPLEMVYV 583
           ET+ L L+VDGID  + NWIK + Y+FLYGGDD++WIR+FT  AK  A+ + + LEM YV
Sbjct: 514 ETFSLNLIVDGIDSVIFNWIKPDNYIFLYGGDDLDWIRRFTMAAKATAKDSNVNLEMAYV 573

Query: 584 GK--SSKRERVKKIITTITTEKLGYCWQDLTMIWFFWTRIESMLFSKIQLGKADDCDPLM 643
           GK   S RE++++I   I +E L + W +  ++WFFWTR+ESML+SKIQLGKADD D +M
Sbjct: 574 GKRNHSHREQIRRISEVIRSENLSHSWAEPALMWFFWTRLESMLYSKIQLGKADDHDDVM 633

Query: 644 QEIKKLLSYDKEGGWAVLSKGSTVILNGHSTTVLPTLGSYD-SWKQQAAEKGFDIAFKNH 703
           Q IKK+LSYDK GGWA+LSKG  +++  H   +  T+  YD +WK     KG+  A  +H
Sbjct: 634 QGIKKILSYDKLGGWALLSKGPEIVMIAHG-AIERTMSVYDRTWKTHVPTKGYTKAMSDH 693

Query: 704 HDE--LQGITHPCCRFEFPHT--TGRIPENFKCPECDRQMEKLTTFLCCHDENSDE 747
           H +  L+    PC  F+F  T  +GRIPE   C EC R MEK  +F CCHDE   E
Sbjct: 694 HHDEVLRETGKPCGHFDFHITARSGRIPEKMNCFECQRPMEKYMSFSCCHDEKLHE 734

BLAST of Lsi05G004670 vs. TAIR10
Match: AT3G01670.1 (AT3G01670.1 unknown protein)

HSP 1 Score: 434.1 bits (1115), Expect = 1.7e-121
Identity = 265/738 (35.91%), Postives = 398/738 (53.93%), Query Frame = 1

Query: 28  KFSLASRHQLAKGNKFLSAISDDNVMMKQIIATHDPDDRDVDTRSLLRLVENILKRATLA 87
           +F    +    +  + + ++SDD VM  +++ TH PD    D  SLL +V +I K    +
Sbjct: 117 RFGPGKKQAFHRNGRPMFSLSDDRVMADRVLKTHSPDMIFFDVTSLLSVVNDIFKSHVPS 176

Query: 88  ADATVWNESLPTKQGSYEQLESLEETGTHQAGFTTMLETLSYTIDRISSEISYKALEGID 147
            D++    SL  K           +   H     T  ET +  ID+IS EI  K L G +
Sbjct: 177 IDSSAPKPSLVFK-----------DYADH-----TSFETFADLIDQISCEIDCKCLHGGE 236

Query: 148 PHA-------------TTLAIFNMLASYRWDAKLVLTLAAFALNYGEFWLLAQIYSENQL 207
            H              TT ++ ++++ YRWDAKLVL L+A A+ YG F LLA+ ++ NQL
Sbjct: 237 SHGMMTSGLHLDSRNTTTFSVLSLVSKYRWDAKLVLVLSALAVKYGVFLLLAETHATNQL 296

Query: 208 AKAMAILKQLPGIFEHSIALKPKFDALKDLVAAILDVTWCIIDLKELPSAYISQEVPAMS 267
            K++A++KQLP IF    AL  + D  + L+  ++D+T  IID+ +LP  +I+      +
Sbjct: 297 TKSLALIKQLPSIFSRQNALHQRLDKTRILMQDMVDLTTTIIDIYQLPPNHIT------A 356

Query: 268 TAVAHIPTAVYWTIRSIVSSATQITSLTSMGYELALSTSTDSWELSTLAHKLKNIRDHLK 327
               HIPTAVYW +R ++   + I+  +    +  +S    S E+   + +L+ I  +L 
Sbjct: 357 AFTDHIPTAVYWIVRCVLICVSHISGASGFKQDQIMSFMEVS-EIHENSERLRKINAYLL 416

Query: 328 KKLVLCHQYIEEKRDIESFQMLINLLE-MTHLDNMKVLKALIYAKDDLQSLVDGSTGQRV 387
           ++       IEE    E +Q LI     + H+D +  L  L+   D L     G + +RV
Sbjct: 417 EQFKKSKMTIEEGIIEEEYQELIQTFTTIIHVDVVPPLLRLLRPIDFLYHGA-GVSKRRV 476

Query: 388 NLDVLKRKNVLLLISDLNISHDELSILDQLYNESRAQGMRVESQFEVVWIPIVDHSIKWN 447
            ++VL +K+VLLLISDL     EL IL+ LY E+  Q       FE++W+P+ D    W 
Sbjct: 477 GINVLTQKHVLLLISDLENIEKELYILESLYTEAWQQS------FEILWVPVQDF---WT 536

Query: 448 DSMQKRFEYLLSIMPWHIVHHPTLISKAVTRFIGEVWQFRNKPILVVLDPQGKVVSPNAI 507
           ++   +FE L   M W+++  P  + +A  RF+ E W F+N+PILV LDP+G+V+S NA 
Sbjct: 537 EADDAKFEALHMNMRWYVLGEPRKLRRAAIRFVREWWGFKNRPILVALDPKGQVMSTNAF 596

Query: 508 HMMWIWGSLAFPFTSVKEEVLWREETWRLELLVDGIDPAVLNWIKEERYVFLYGGDDIEW 567
            M+WIW   A PFT+ +E  LW E+ W LE L+DG DP  LN + + +Y+ LYGG+D++W
Sbjct: 597 PMVWIWQPFAHPFTTARERDLWSEQEWNLEFLIDGTDPHSLNQLVDGKYICLYGGEDMQW 656

Query: 568 IRKFTTTAKTVAQAARIPLEMVYVGKSSKRERVKKIITTITTEKLGYCWQDLTMIWFFWT 627
           I+ FT+  + VA+AA I LEMVYVGK + +  ++ II TI  E L +   DL  IWFFWT
Sbjct: 657 IKNFTSLWRNVAKAANIQLEMVYVGKRNPKNGIQPIINTIREENLSHTLPDLFQIWFFWT 716

Query: 628 RIESMLFSKIQLGKA-----------DDCDPLMQEIKKLLSYDKEG-GWAVLSKGSTVIL 687
           R+ESM  SK ++ KA           ++ D ++QE+  +L Y  EG GW ++SK S +++
Sbjct: 717 RVESMWESKQRMLKAHGIKGREGFKEEEKDLVLQEVVAMLGYGGEGDGWGLVSKASDMMV 776

Query: 688 NGHSTTVLPTLGSYDSWKQQAAEKGFDIAFKNHHDELQGITHPCCRFEFPHTTGRIPENF 740
                     L  ++ W+     KGF  A  N H  ++   H C RF  P T G IP   
Sbjct: 777 RAKGNLFSRGLAEFNEWEVNIPTKGFLTAL-NDHLLMRLPPHHCTRFMLPETAGIIPNEV 820

BLAST of Lsi05G004670 vs. TAIR10
Match: AT1G67790.1 (AT1G67790.1 unknown protein)

HSP 1 Score: 157.5 bits (397), Expect = 3.1e-38
Identity = 104/403 (25.81%), Postives = 197/403 (48.88%), Query Frame = 1

Query: 340 LEMTHLDNMKVLKALIYAKDDLQSLVDGSTGQRVNLDVLKRKNVLLLISDLNISHDELSI 399
           L   +L   +V+K+ +     +Q +      Q++++  ++ K  LLL+S   +      +
Sbjct: 200 LSNIYLTTYRVVKSALTC---MQQIPYFKQTQQISITEVQDKVTLLLLSKPPVE-PLFFL 259

Query: 400 LDQLYNESRAQGMRVESQFEVVWIPIVDHSIKWNDSMQKRFEYLLSIMPWHIVHHPTLIS 459
           L QLY+         E  +E++W+PI   S KW D  ++ F++  + +PW  V  P L+S
Sbjct: 260 LQQLYDHP--SNTNTEQNYEIIWVPIPS-SQKWTDEEKEIFDFYSNSLPWISVRQPWLMS 319

Query: 460 KAVTRFIGEVWQFR-NKPILVVLDPQGKVVSPNAIHMMWIWGSLAFPFTSVKEEVLWREE 519
             +  F  + W ++ N+ +LVV+D  G+ V+ NA+ M+ IWG  A+PF+  +E+ LW+E 
Sbjct: 320 STILNFFKQEWHYKDNEAMLVVIDSNGRFVNMNAMDMVLIWGVKAYPFSVSREDELWKEH 379

Query: 520 TWRLELLVDGIDPAVLNWIKEERYVFLYGGDDIEWIRKFTTTAKTVAQAARIPLEMVYVG 579
            W + LL+DGI P       E R + ++G ++++WI +F + A+ + Q     LE++Y+ 
Sbjct: 380 GWSINLLLDGIHPTF-----EGREICIFGSENLDWIDEFVSLARKI-QNLGFQLELIYLS 439

Query: 580 KSSKRERVKKIITTITTEKLGYCWQDLTMIWFFWTRIESMLFSKIQ--LGKADDCDPLMQ 639
              + ER  +  + + +          T+   FW R+ES+  SK++  + +    D + +
Sbjct: 440 NQRRDERAMEESSILFSP---------TLQQLFWLRLESIERSKLKRIVIEPSKPDRVFE 499

Query: 640 EIKKLLSYD--KEGGWAVLSKGSTV-ILNGHSTTVLPTLGSYDSWKQQAAEKGFDIAFKN 699
           E++ LL +D  K  GW ++  GST   ++G   T    +     W + A   GF  A + 
Sbjct: 500 EVRNLLDFDYGKHRGWGIIGNGSTAETVDGEKMT--ERMRKIVRWGEYAKGLGFTEAIEI 559

Query: 700 HHDELQGITHPCCRFEFPHTTGRIPENFKCPECDRQMEKLTTF 737
             ++   ++H       P       +   C +C   M++   +
Sbjct: 560 AAEKPCELSHTAV---VPFEEALTMKVVTCEKCKWPMKRFVAY 575

BLAST of Lsi05G004670 vs. NCBI nr
Match: gi|449455904|ref|XP_004145690.1| (PREDICTED: uncharacterized protein LOC101221710 [Cucumis sativus])

HSP 1 Score: 1377.8 bits (3565), Expect = 0.0e+00
Identity = 686/724 (94.75%), Postives = 702/724 (96.96%), Query Frame = 1

Query: 23  MDGSNKFSLASRHQLAKGNKFLSAISDDNVMMKQIIATHDPDDRDVDTRSLLRLVENILK 82
           MD  NKFSLASRHQLAKGNKFLSAISDDNVMMKQI+ATHDPDDRDVDTRSLLRLVENILK
Sbjct: 1   MDSGNKFSLASRHQLAKGNKFLSAISDDNVMMKQILATHDPDDRDVDTRSLLRLVENILK 60

Query: 83  RATLAADATVWNESLPTKQGSYEQLESLEETGTHQAGFTTMLETLSYTIDRISSEISYKA 142
           RATLAADAT          GSYEQLESLEETGTHQAG TTMLE LSYTIDRISSEISYKA
Sbjct: 61  RATLAADAT----------GSYEQLESLEETGTHQAGLTTMLEALSYTIDRISSEISYKA 120

Query: 143 LEGIDPHATTLAIFNMLASYRWDAKLVLTLAAFALNYGEFWLLAQIYSENQLAKAMAILK 202
           LEGIDPHATTLAIFNMLASYRWDAKLVLTLAAFALNYGEFWLLAQIYS+NQLAKAMAILK
Sbjct: 121 LEGIDPHATTLAIFNMLASYRWDAKLVLTLAAFALNYGEFWLLAQIYSQNQLAKAMAILK 180

Query: 203 QLPGIFEHSIALKPKFDALKDLVAAILDVTWCIIDLKELPSAYISQEVPAMSTAVAHIPT 262
           QLPGIFEHSIALKPKFDALK+LVAAILDVTWCIIDLKELPSAYISQEVPAMSTAVAHIPT
Sbjct: 181 QLPGIFEHSIALKPKFDALKELVAAILDVTWCIIDLKELPSAYISQEVPAMSTAVAHIPT 240

Query: 263 AVYWTIRSIVSSATQITSLTSMGYELALSTSTDSWELSTLAHKLKNIRDHLKKKLVLCHQ 322
           AVYWTIRSIVSSATQITSLTSMGYELALSTSTD+WELSTLAHKLKNI DHLKKKLVLCHQ
Sbjct: 241 AVYWTIRSIVSSATQITSLTSMGYELALSTSTDAWELSTLAHKLKNICDHLKKKLVLCHQ 300

Query: 323 YIEEKRDIESFQMLINLLEMTHLDNMKVLKALIYAKDDLQSLVDGSTGQRVNLDVLKRKN 382
           +IEEK+DIESFQMLINL EM HLDNMKVLKALIY KDDLQ LVDGSTGQRVNLDVLKRKN
Sbjct: 301 HIEEKKDIESFQMLINLFEMNHLDNMKVLKALIYPKDDLQPLVDGSTGQRVNLDVLKRKN 360

Query: 383 VLLLISDLNISHDELSILDQLYNESRAQGMRVESQFEVVWIPIVDHSIKWNDSMQKRFEY 442
           VLLLISDLNISHDELSILDQLYNESRAQGMRVESQFEVVWIPIVDHSIKWNDSMQKRFEY
Sbjct: 361 VLLLISDLNISHDELSILDQLYNESRAQGMRVESQFEVVWIPIVDHSIKWNDSMQKRFEY 420

Query: 443 LLSIMPWHIVHHPTLISKAVTRFIGEVWQFRNKPILVVLDPQGKVVSPNAIHMMWIWGSL 502
           LLSIMPWHIVHHPTLISKAVTRFIGEVWQFRN+PILVVLDPQGKVVSPNAIHMMWIWGSL
Sbjct: 421 LLSIMPWHIVHHPTLISKAVTRFIGEVWQFRNRPILVVLDPQGKVVSPNAIHMMWIWGSL 480

Query: 503 AFPFTSVKEEVLWREETWRLELLVDGIDPAVLNWIKEERYVFLYGGDDIEWIRKFTTTAK 562
           AFPFTSVKEEVLW+EETWRLELLVDGIDPAVLNWIKEERY+FLYGGDDIEWIRKFTTTAK
Sbjct: 481 AFPFTSVKEEVLWKEETWRLELLVDGIDPAVLNWIKEERYIFLYGGDDIEWIRKFTTTAK 540

Query: 563 TVAQAARIPLEMVYVGKSSKRERVKKIITTITTEKLGYCWQDLTMIWFFWTRIESMLFSK 622
           TVAQAARIPLEMVYVGKSSKRERVKKIITTITTEKLGYCWQDLTMIWFFWTRIESML+SK
Sbjct: 541 TVAQAARIPLEMVYVGKSSKRERVKKIITTITTEKLGYCWQDLTMIWFFWTRIESMLYSK 600

Query: 623 IQLGKADDCDPLMQEIKKLLSYDKEGGWAVLSKGSTVILNGHSTTVLPTLGSYDSWKQQA 682
           IQLGKADDCDPLMQEIKKLLSYDKEGGWAVLSKGS VILNGHSTT+LPTLGS+DSWKQ+A
Sbjct: 601 IQLGKADDCDPLMQEIKKLLSYDKEGGWAVLSKGSNVILNGHSTTMLPTLGSFDSWKQEA 660

Query: 683 AEKGFDIAFKNHHDELQGITHPCCRFEFPHTTGRIPENFKCPECDRQMEKLTTFLCCHDE 742
            +KGFDIAFKNHHDELQGITHPCCRFEFPHT+GRIPENFKCP CDRQMEKLTTFLCCHDE
Sbjct: 661 TDKGFDIAFKNHHDELQGITHPCCRFEFPHTSGRIPENFKCPGCDRQMEKLTTFLCCHDE 714

Query: 743 NSDE 747
           NS+E
Sbjct: 721 NSNE 714

BLAST of Lsi05G004670 vs. NCBI nr
Match: gi|659098146|ref|XP_008449999.1| (PREDICTED: uncharacterized protein LOC103491716 [Cucumis melo])

HSP 1 Score: 1370.9 bits (3547), Expect = 0.0e+00
Identity = 682/724 (94.20%), Postives = 700/724 (96.69%), Query Frame = 1

Query: 23  MDGSNKFSLASRHQLAKGNKFLSAISDDNVMMKQIIATHDPDDRDVDTRSLLRLVENILK 82
           MD  NKFSLASRHQLAKGNKFLSAISDDNVMMKQI+ATHDPDDRDVDTRSLLRLVENILK
Sbjct: 1   MDCGNKFSLASRHQLAKGNKFLSAISDDNVMMKQILATHDPDDRDVDTRSLLRLVENILK 60

Query: 83  RATLAADATVWNESLPTKQGSYEQLESLEETGTHQAGFTTMLETLSYTIDRISSEISYKA 142
           RATLAADAT          GSYEQLES+EETGT+QAG TTMLE LSYTIDRISSEISYKA
Sbjct: 61  RATLAADAT----------GSYEQLESMEETGTNQAGLTTMLEALSYTIDRISSEISYKA 120

Query: 143 LEGIDPHATTLAIFNMLASYRWDAKLVLTLAAFALNYGEFWLLAQIYSENQLAKAMAILK 202
           LEGIDPHATTLAIFNMLASY W AKLVLTLAAFALNYGEFWLLAQIYS+NQLAKAMAILK
Sbjct: 121 LEGIDPHATTLAIFNMLASYEWGAKLVLTLAAFALNYGEFWLLAQIYSQNQLAKAMAILK 180

Query: 203 QLPGIFEHSIALKPKFDALKDLVAAILDVTWCIIDLKELPSAYISQEVPAMSTAVAHIPT 262
           QLPGIFEHS+ALKPKFDALKDLVAAIL+VTWC+IDLKELPSAYISQEVPAMSTAVAHIPT
Sbjct: 181 QLPGIFEHSVALKPKFDALKDLVAAILNVTWCVIDLKELPSAYISQEVPAMSTAVAHIPT 240

Query: 263 AVYWTIRSIVSSATQITSLTSMGYELALSTSTDSWELSTLAHKLKNIRDHLKKKLVLCHQ 322
           AVYWTIRSIVS ATQITSLTSMGYELALSTSTDSWELSTLAHKLKNI DHLKKKLVLCHQ
Sbjct: 241 AVYWTIRSIVSCATQITSLTSMGYELALSTSTDSWELSTLAHKLKNICDHLKKKLVLCHQ 300

Query: 323 YIEEKRDIESFQMLINLLEMTHLDNMKVLKALIYAKDDLQSLVDGSTGQRVNLDVLKRKN 382
           YIEEK+D ESFQML+NL  MTHLDNMKVLKALIY KDDLQ LVDGSTGQRVNLDVLKRKN
Sbjct: 301 YIEEKKDTESFQMLVNLFGMTHLDNMKVLKALIYPKDDLQPLVDGSTGQRVNLDVLKRKN 360

Query: 383 VLLLISDLNISHDELSILDQLYNESRAQGMRVESQFEVVWIPIVDHSIKWNDSMQKRFEY 442
           VLLLISDLNISHDELSILDQLYNESRAQGMRVESQFEVVWIPIVDHSIKWNDSMQKRFEY
Sbjct: 361 VLLLISDLNISHDELSILDQLYNESRAQGMRVESQFEVVWIPIVDHSIKWNDSMQKRFEY 420

Query: 443 LLSIMPWHIVHHPTLISKAVTRFIGEVWQFRNKPILVVLDPQGKVVSPNAIHMMWIWGSL 502
           LLSIMPWHIVHHPTLISKAVTRFIGEVWQFRN+PILVVLDPQGKVVSPNAIHMMWIWGSL
Sbjct: 421 LLSIMPWHIVHHPTLISKAVTRFIGEVWQFRNRPILVVLDPQGKVVSPNAIHMMWIWGSL 480

Query: 503 AFPFTSVKEEVLWREETWRLELLVDGIDPAVLNWIKEERYVFLYGGDDIEWIRKFTTTAK 562
           AFPFTSVKEEVLW+EETWRLELLVDGIDPAVLNWIKEERY+FLYGGDDIEWIRKFTTTAK
Sbjct: 481 AFPFTSVKEEVLWKEETWRLELLVDGIDPAVLNWIKEERYIFLYGGDDIEWIRKFTTTAK 540

Query: 563 TVAQAARIPLEMVYVGKSSKRERVKKIITTITTEKLGYCWQDLTMIWFFWTRIESMLFSK 622
           TVAQAARIPLEMVYVGKSSKRERVKKIITTITTEKLGYCWQDLTMIWFFWTRIESML+SK
Sbjct: 541 TVAQAARIPLEMVYVGKSSKRERVKKIITTITTEKLGYCWQDLTMIWFFWTRIESMLYSK 600

Query: 623 IQLGKADDCDPLMQEIKKLLSYDKEGGWAVLSKGSTVILNGHSTTVLPTLGSYDSWKQQA 682
           IQLGKADDCDPLMQEIKKLLSYDKEGGWAVLSKGS VILNGHSTTVLPTLGS+DSWKQ+A
Sbjct: 601 IQLGKADDCDPLMQEIKKLLSYDKEGGWAVLSKGSNVILNGHSTTVLPTLGSFDSWKQEA 660

Query: 683 AEKGFDIAFKNHHDELQGITHPCCRFEFPHTTGRIPENFKCPECDRQMEKLTTFLCCHDE 742
           A+KGFDIAFKNHHDELQGITHPCCRFEFPHT+GRIPENFKCPECDRQMEKLTTFLCCHDE
Sbjct: 661 ADKGFDIAFKNHHDELQGITHPCCRFEFPHTSGRIPENFKCPECDRQMEKLTTFLCCHDE 714

Query: 743 NSDE 747
           NS+E
Sbjct: 721 NSNE 714

BLAST of Lsi05G004670 vs. NCBI nr
Match: gi|802539547|ref|XP_012071551.1| (PREDICTED: uncharacterized protein LOC105633544 [Jatropha curcas])

HSP 1 Score: 1008.1 bits (2605), Expect = 8.3e-291
Identity = 480/711 (67.51%), Postives = 590/711 (82.98%), Query Frame = 1

Query: 36  QLAKGNKFLSAISDDNVMMKQIIATHDPDDRDVDTRSLLRLVENILKRATLAADATVWNE 95
           QL KG++ + A+SD+N M+KQI ATH PD R+ D R LL +V++IL RATL  D+ +   
Sbjct: 18  QLIKGDRSMLALSDENSMLKQIQATHAPDGREFDVRPLLNIVDDILSRATLHGDSALM-- 77

Query: 96  SLPTKQGSYEQLESLEETGTHQAGFTTMLETLSYTIDRISSEISYKALEGIDPHATTLAI 155
                     Q  +  +    QA F  MLE LS+ IDRI+SEISYKAL G D HATTL++
Sbjct: 78  --------VNQTHAEVDDKNRQANFIAMLEALSFVIDRIASEISYKALSGADAHATTLSL 137

Query: 156 FNMLASYRWDAKLVLTLAAFALNYGEFWLLAQIYSENQLAKAMAILKQLPGIFEHSIALK 215
           FNML+SY WDAK+VLT+AAFALNYGEFWLLAQIYS N LAK+MAILKQLP I EH+  LK
Sbjct: 138 FNMLSSYSWDAKVVLTMAAFALNYGEFWLLAQIYSSNPLAKSMAILKQLPVILEHTGPLK 197

Query: 216 PKFDALKDLVAAILDVTWCIIDLKELPSAYISQEVPAMSTAVAHIPTAVYWTIRSIVSSA 275
           P+FDAL +L++ ++DVT CI++ KELPS+YIS EVPA+STA+AHIPTAVYWT+RS+V+ A
Sbjct: 198 PRFDALNNLISVMMDVTKCIVEFKELPSSYISSEVPALSTAIAHIPTAVYWTMRSVVACA 257

Query: 276 TQITSLTSMGYELALSTSTDSWELSTLAHKLKNIRDHLKKKLVLCHQYIEEKRDIESFQM 335
           TQIT+LT+MG+E A   +T++WELSTLAHKL+NI +HL+K+L +C+Q+IEEKR++E++Q 
Sbjct: 258 TQITNLTTMGHEFA---TTEAWELSTLAHKLQNILEHLRKQLSICYQHIEEKRNVEAYQA 317

Query: 336 LINLLEMTHLDNMKVLKALIYAKDDLQSLVDGSTGQRVNLDVLKRKNVLLLISDLNISHD 395
           L+NL +  H+DNMK+LKALIYAKDD+Q LVDGST +RVN+DVL+RKNVLLLIS L+ISHD
Sbjct: 318 LLNLFDTIHIDNMKILKALIYAKDDIQPLVDGSTKRRVNVDVLRRKNVLLLISGLDISHD 377

Query: 396 ELSILDQLYNESRAQGMRVESQFEVVWIPIVDHSIKWNDSMQKRFEYLLSIMPWHIVHHP 455
           ELSIL+Q+YNESR    R+ESQ+EVVW+P+VD ++ + D+M K+FE L ++MPW+ V+HP
Sbjct: 378 ELSILEQIYNESRIHATRLESQYEVVWVPVVDRTVPYTDAMHKQFESLQTVMPWYTVYHP 437

Query: 456 TLISKAVTRFIGEVWQFRNKPILVVLDPQGKVVSPNAIHMMWIWGSLAFPFTSVKEEVLW 515
           TLI K V RFI EVW FR +PILVVLDPQGKVV PNA+HMMWIW S AFPFTS +EE LW
Sbjct: 438 TLIEKVVIRFIKEVWHFRRRPILVVLDPQGKVVCPNALHMMWIWASNAFPFTSYREESLW 497

Query: 516 REETWRLELLVDGIDPAVLNWIKEERYVFLYGGDDIEWIRKFTTTAKTVAQAARIPLEMV 575
           +EETWRLELL+DGIDP +LNWIKE +Y+FLYGGDD+EWIRKFT TA+ VAQAARI LEMV
Sbjct: 498 KEETWRLELLIDGIDPTILNWIKEGKYIFLYGGDDVEWIRKFTNTARAVAQAARISLEMV 557

Query: 576 YVGKSSKRERVKKIITTITTEKLGYCWQDLTMIWFFWTRIESMLFSKIQLGKADDCDPLM 635
           YVGKS KR++V++II+TIT EKL Y WQDLTMIWFFWTR++SML+SKIQLGK D+ DP+M
Sbjct: 558 YVGKSCKRDKVQRIISTITVEKLSYVWQDLTMIWFFWTRLQSMLYSKIQLGKIDEHDPMM 617

Query: 636 QEIKKLLSYDKEGGWAVLSKGSTVILNGHSTTVLPTLGSYDSWKQQAAEKGFDIAFKNHH 695
           QEIKKLLSYDKEGGWAVLS+GS +++NGHSTTVLPTL  YD WK+  + KGFD++FK HH
Sbjct: 618 QEIKKLLSYDKEGGWAVLSRGSNIVVNGHSTTVLPTLLEYDIWKENVSLKGFDLSFKEHH 677

Query: 696 DELQGITHPCCRFEFPHTTGRIPENFKCPECDRQMEKLTTFLCCHDENSDE 747
           D+L GI HPCCRFEFP TTGRIPE  KCPEC+R MEK  TFLCCH+E + E
Sbjct: 678 DKLHGIAHPCCRFEFPSTTGRIPERLKCPECNRNMEKHITFLCCHEEETSE 715

BLAST of Lsi05G004670 vs. NCBI nr
Match: gi|743939366|ref|XP_011014132.1| (PREDICTED: uncharacterized protein LOC105117991 [Populus euphratica])

HSP 1 Score: 1005.7 bits (2599), Expect = 4.1e-290
Identity = 489/711 (68.78%), Postives = 586/711 (82.42%), Query Frame = 1

Query: 32  ASRHQLAKGNKFLSAISDDNVMMKQIIATHDPDDRDVDTRSLLRLVENILKRATLAADAT 91
           AS+  +      +  +SDDNVMMKQI+ TH PD R+VD + LL LVE+ILKRATL  D+ 
Sbjct: 16  ASQQLIKSDRGSMLTMSDDNVMMKQIVGTHAPDGREVDVKPLLLLVEDILKRATLQIDS- 75

Query: 92  VWNESLPTKQGSYEQLESLEETGTHQAGFTTMLETLSYTIDRISSEISYKALEGIDPHAT 151
               SL T Q   E      E  T+   F +ML+ LSYTIDRISSEI+YKAL G D HAT
Sbjct: 76  ----SLTTSQAQAEM-----EDKTYHVNFVSMLDALSYTIDRISSEIAYKALGGTDAHAT 135

Query: 152 TLAIFNMLASYRWDAKLVLTLAAFALNYGEFWLLAQIYSENQLAKAMAILKQLPGIFEHS 211
           T+++FNML SY WDAKLVLTLAAFALNYGEFWLLAQI S NQLAK+MAILKQLP I EHS
Sbjct: 136 TVSLFNMLTSYSWDAKLVLTLAAFALNYGEFWLLAQISSSNQLAKSMAILKQLPSIMEHS 195

Query: 212 IALKPKFDALKDLVAAILDVTWCIIDLKELPSAYISQEVPAMSTAVAHIPTAVYWTIRSI 271
             LKP+FDA+ +L+  ++DV  C+++ K+LP AYIS EVPA+STA+AHIPTAVYWTIRS+
Sbjct: 196 GPLKPRFDAINNLIKVMMDVARCVVEFKDLPPAYISNEVPALSTAMAHIPTAVYWTIRSV 255

Query: 272 VSSATQITSLTSMGYELALSTSTDSWELSTLAHKLKNIRDHLKKKLVLCHQYIEEKRDIE 331
           V+ A QITSLT+MG+E ++ST TD+WELSTLAHKL  I DHL+K+L  C+QYI+EKR++E
Sbjct: 256 VACAAQITSLTTMGHEFSIST-TDAWELSTLAHKLSYILDHLRKQLDTCYQYIDEKRNVE 315

Query: 332 SFQMLINLLEMTHLDNMKVLKALIYAKDDLQSLVDGSTGQRVNLDVLKRKNVLLLISDLN 391
           SFQML NL EM H+DNMKVLKALIYAKDD+Q L+DGS+ +RV+LDVL+RKNVLLLIS L 
Sbjct: 316 SFQMLKNLFEMIHIDNMKVLKALIYAKDDIQPLIDGSSKKRVHLDVLRRKNVLLLISGLE 375

Query: 392 ISHDELSILDQLYNESRAQGMRVESQFEVVWIPIVDHSIKWNDSMQKRFEYLLSIMPWHI 451
           +S DELSIL+Q+YNESR  G R++SQ+EVVW+PIVD S++WND M+++FE + S MPW  
Sbjct: 376 MSTDELSILEQIYNESRHHGPRLDSQYEVVWVPIVDRSVQWNDPMKEKFESMQSSMPWFT 435

Query: 452 VHHPTLISKAVTRFIGEVWQFRNKPILVVLDPQGKVVSPNAIHMMWIWGSLAFPFTSVKE 511
           V+HP LI KAV RFI EVW FRNKPILVVLDPQGKVV PNA+HMMWIWGS AFPFTS++E
Sbjct: 436 VYHPLLIEKAVIRFIKEVWHFRNKPILVVLDPQGKVVCPNALHMMWIWGSNAFPFTSLRE 495

Query: 512 EVLWREETWRLELLVDGIDPAVLNWIKEERYVFLYGGDDIEWIRKFTTTAKTVAQAARIP 571
           E LW++ETWRLELLVDGIDP +LNWI E +Y+FLYGGDD EW+RKFT++A+ VAQAARIP
Sbjct: 496 ESLWKDETWRLELLVDGIDPVILNWINEGKYIFLYGGDDDEWVRKFTSSARAVAQAARIP 555

Query: 572 LEMVYVGKSSKRERVKKIITTITTEKLGYCWQDLTMIWFFWTRIESMLFSKIQLGKADDC 631
           LE+VYVGKSSKRE+++++I TIT EKL Y WQDLTM+WFFWTR+ESML+SKIQLGK DD 
Sbjct: 556 LEIVYVGKSSKREKIRRVIATITVEKLSYVWQDLTMMWFFWTRLESMLYSKIQLGKLDDH 615

Query: 632 DPLMQEIKKLLSYDKEGGWAVLSKGSTVILNGHSTTVLPTLGSYDSWKQQAAEKGFDIAF 691
           DP+MQEIKKLLSY +EGGWAVLSKGS V++NGH TTVL TL  YD WK+Q   KGFD+AF
Sbjct: 616 DPMMQEIKKLLSYGREGGWAVLSKGSNVVVNGHKTTVLQTLLEYDLWKEQVPVKGFDMAF 675

Query: 692 KNHHDELQGITHPCCRFEFPHTTGRIPENFKCPECDRQMEKLTTFLCCHDE 743
           ++HH +L+GI  PCCRF+FP TTGRIPE  KCPEC+  MEK +TFLCCHDE
Sbjct: 676 QDHHFQLRGIARPCCRFDFPMTTGRIPETMKCPECNSAMEKFSTFLCCHDE 715

BLAST of Lsi05G004670 vs. NCBI nr
Match: gi|566212619|ref|XP_002324001.2| (hypothetical protein POPTR_0017s10720g [Populus trichocarpa])

HSP 1 Score: 1005.4 bits (2598), Expect = 5.4e-290
Identity = 482/706 (68.27%), Postives = 582/706 (82.44%), Query Frame = 1

Query: 36  QLAKGNKFLSAISDDNVMMKQIIATHDPDDRDVDTRSLLRLVENILKRATLAADATVWNE 95
           QL + ++ +  +SDDNVMMK+I+ TH PD R+VD + LL LVE+ILKRATL  D      
Sbjct: 20  QLIRSDRSMITMSDDNVMMKRIVETHAPDGREVDVKPLLHLVEDILKRATLQTDT----- 79

Query: 96  SLPTKQGSYEQLESLEETGTHQAGFTTMLETLSYTIDRISSEISYKALEGIDPHATTLAI 155
           SL T Q   E      E  T+ A F  ML++LSYTIDRIS EI+YK   G D HATT+ +
Sbjct: 80  SLTTSQAHAES-----EDKTNHASFAVMLDSLSYTIDRISCEIAYKG--GADGHATTVEL 139

Query: 156 FNMLASYRWDAKLVLTLAAFALNYGEFWLLAQIYSENQLAKAMAILKQLPGIFEHSIALK 215
           FNMLASY WDAKLVLTLAAFALNYGEFWLLAQIYS NQLAK+MAILKQLP I EHS  LK
Sbjct: 140 FNMLASYSWDAKLVLTLAAFALNYGEFWLLAQIYSSNQLAKSMAILKQLPNILEHSGPLK 199

Query: 216 PKFDALKDLVAAILDVTWCIIDLKELPSAYISQEVPAMSTAVAHIPTAVYWTIRSIVSSA 275
           P+FDAL +L+  ++DVT C+++ K+LP  YIS EV A+S A+AH+PTAVYWT+RS+++ A
Sbjct: 200 PRFDALNNLIKVMMDVTRCVVEFKDLPPTYISHEVSALSAAMAHVPTAVYWTVRSVLACA 259

Query: 276 TQITSLTSMGYELALSTSTDSWELSTLAHKLKNIRDHLKKKLVLCHQYIEEKRDIESFQM 335
            QITSLT+MGYE ++ST T +WELSTLAHKL NI +HL+++L  C+QYI+EKR++E++QM
Sbjct: 260 AQITSLTTMGYEFSIST-TKAWELSTLAHKLSNILEHLRRQLATCYQYIDEKRNVEAYQM 319

Query: 336 LINLLEMTHLDNMKVLKALIYAKDDLQSLVDGSTGQRVNLDVLKRKNVLLLISDLNISHD 395
           L+NL EM H+DNMKVLKALIYAKDD+Q L+DGS  +RV+LDVL+RKNVLLLIS L+IS+D
Sbjct: 320 LLNLFEMIHIDNMKVLKALIYAKDDIQPLIDGSNKRRVHLDVLRRKNVLLLISGLDISND 379

Query: 396 ELSILDQLYNESRAQGMRVESQFEVVWIPIVDHSIKWNDSMQKRFEYLLSIMPWHIVHHP 455
           EL+IL+Q+YNES   G R++SQ+++VWIPI DHS++W D ++++FE L + MPW+ V+HP
Sbjct: 380 ELAILEQIYNESSHHGTRLDSQYDLVWIPITDHSVQWTDPLKEKFESLQNSMPWYTVYHP 439

Query: 456 TLISKAVTRFIGEVWQFRNKPILVVLDPQGKVVSPNAIHMMWIWGSLAFPFTSVKEEVLW 515
           +LI KA  RFI EVW FRNKPILVVLDPQGKVVSPNA+HMMWIWGS AFPFTS++EE LW
Sbjct: 440 SLIDKAAIRFIREVWHFRNKPILVVLDPQGKVVSPNALHMMWIWGSNAFPFTSLREESLW 499

Query: 516 REETWRLELLVDGIDPAVLNWIKEERYVFLYGGDDIEWIRKFTTTAKTVAQAARIPLEMV 575
           REETWRLELLVDGIDP +LNWIKEE+Y+F+YGGDD+EW+RKFT TA+ VAQAARIPLEMV
Sbjct: 500 REETWRLELLVDGIDPVILNWIKEEKYIFMYGGDDVEWVRKFTNTARAVAQAARIPLEMV 559

Query: 576 YVGKSSKRERVKKIITTITTEKLGYCWQDLTMIWFFWTRIESMLFSKIQLGKADDCDPLM 635
           YVGKS KRE++++++ TI  EKL Y WQDLTMIWFFWTR+ESMLFSKIQLGK DD DP+M
Sbjct: 560 YVGKSRKREQIRRVMGTINVEKLSYAWQDLTMIWFFWTRLESMLFSKIQLGKVDDHDPMM 619

Query: 636 QEIKKLLSYDKEGGWAVLSKGSTVILNGHSTTVLPTLGSYDSWKQQAAEKGFDIAFKNHH 695
           Q IKKLLSYD+EGGWAVLSKGS+V++NGH TTVLPTL  YD WK+Q   KGFD+AF+ HH
Sbjct: 620 QAIKKLLSYDREGGWAVLSKGSSVVVNGHGTTVLPTLVEYDLWKEQVPVKGFDLAFQEHH 679

Query: 696 DELQGITHPCCRFEFPHTTGRIPENFKCPECDRQMEKLTTFLCCHD 742
             L  I HPC RFEFP T GRIPE  KCPEC+R MEK TTFLCCHD
Sbjct: 680 GNLHDIVHPCSRFEFPMTAGRIPETLKCPECNRSMEKFTTFLCCHD 712