Lsi05G004280 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi05G004280
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionMagnesium transporter NIPA protein
Locationchr05 : 5448521 .. 5454211 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACGGACACGTCCACAAAAATTTAGTGGATCGATATTAAACTCATGATAGCACGGTCCCTCGTTATTAGTGTAGTCCGGAGCTTACCCGATCCATCTTGACCGACAGGTGACAGTTAAGTACGCCGGCCGCGGCCGTGGCTTTGTTCACAATCTTCACACACAGAATCACTCAGTATTCTCTCCCCTTCATCTCTAATTGACCGCACAACTTCCCTCTCTTTCACTTTTCTTCATCACGCTTTCCTTCCTCTCCGCCCTCTCACGACGGAGTCCGGCGGTTTTCTCTTCTCTCTACACTCCGAATTCCCTTGGGAGGGAAGCTACTCACACCCAATTCCTCCCTCCCATTCTTCCCACCTTCATTATATTTCTTCTCAATGCCCGACTTCCTCTCTTGGATTGGAACTTGATCTCAACTGCCCTCCATTCTCCACTGACGGATCTTCTCACAGGTAAACCCTCGCCCCCAGCTATCAATTCTTGCTTTTCTTTAATCTCTTTTTCATTTTGTTTTCGATCAGCTTTACTTTTTCTTCCTTGCCTCCCAATTTTTCTTTCCTTCTATTTGACTCTCTGGATTTAGATCCATAGTTTTGGCTTCAGATCTTGATCAGGAACTTGACCCTTTTCATCCCTCGTTTCCCTGCTGCCTCTATCATCTTTACTTCTATGTTGCCTGTTTCTTTTCCTCTCCTTTTGTCAACTTTTCAGTTTTAGTCCATTTACATTGCTGGACATAGCCCAAATTACTCTCTCTAGGTCCGTTTTTGTTTCTGAATTTGATGAAGTCTGTAAGCTGTAACAGTTGTTTGGCCTAATTGCCTGTGCGTTAATATTAAAAGACCCTCCTGCTCCGGGTCGCAGGGGATTATTATGTTCTGAAATTATTCTGTTCCATTTCGGAATCGAGATTATGCTTTTTGCTCAAACGATTGCGAATTGGTGTTCGGAATTTAGATATCTGCTCGAAATTTTAGGTTCTTTCAACCCATTAACACGTACTCGAGATTCGCCTTTTGAGCTCGTTCTATGTTGCTTTGGAATTCACATAAGCAATTGACAGTGTCTCACTTTTTTGCAATTTGGTCATTCACTTCTCTGCTGCTTTTCAACGACAGGCTGGGATATTCAGCTGTATGCTGATTCGCTTATACCTCTCTAATTTATGGGGGAGTGGGTTATTGGAGCTGTCATCAACCTCTTTGGCAGCATTGCCATCAACTTTGGGACGAACCTTCTTAAATTAGGCCATAACGAGGTTGGCAGAGATCTCTTTTCTTACTTTTGGTCTATACTGTCTAGCTAAGTACTCTTCACCTATTGTTATTGCTGCTGTTCTCTGTAGAAAACATTTCTTATCCACATTTTTCTTGAGGATGCAATACAGACATGCTATAAGAGCTTTTGTGGCAGGTGTCAATGTGTCATGCATCACAAAATATAAATGGCATCTTTCCTTTCAAAAGAAAAAAAAAAGTATTTCACTTTTCCAAAGCTTTAATTGGTTTCGTAAGTAATAGCCCATCAAACTTCTGAGTTCCATAACTAGTTGGACTCGATTATACTTCGCTTGTCCATCACTTGTTATCAGAAGTGTGTTCTGGATATCTGGGTTCCAAGTCTTCATTTTGGCATCATATGTACTATGGTTTTTAAAGAATTTACTCTTGCAGAGAGAGAAGCATTCTATGCTAGAAAACAATGGTTCTAATGGAAAGACTCCAATGAAGCCCATTATCTACTTCCAGACTTGGAGAGTTGGTAAGTCATTTTCTGGTGTTAAATCCTTGTAGGCATTTACATGTTATACTTTTTCTGGAAGAAGACTACATTTGGTACCTAAGGCCTTAGTTATAACTTAACTTATAGGCTTTTATGTACAGGTATTACATTTTTCATTCTCGGAAATTGCCTTAATTTTATTTCCTTCGGATATGCTGCCCAGGTTGGTAGAAATTACTGTATCCCATTTCACAATTTGAGTATATTCATATTATTAGAACATCCCTTCCCTGTGATTTTTGTTCTTTCCAGTGTTCATTAGACGTTTTGCTTCTCTATAGAACGTGGATTTGCTGTTCTATTTTTTCTAATTTTACTTTTCTTCTCCCTTCTTCTTCTTCCCCACCCCTACTCTGCAGTCTCTTCTTGCGGCACTTGGATCAGTTCAGTTTGTGTCCAATATTGCTTTTGCATACTTTGTACTCCACAAGATGGTTACTGTTAAGTATGTAAATATCTGTAATCACACGTCTTTGGAACCATGGAGGCTCACAATACATGTTTTCATCATTATTGTTTCTAATTTAATGGTCAATGACTGTATTGCTTTTTTGCAGGGTAATGGTTGCCACTGCTTTTATTGTTCTTGGAAACGTTTTTCTAGTTGCTTTTGGCAACCATCAGTCCCCTGGTAGATGCCATGCTTTTGATTTATTTATCTTTCAAGTTTTAGTGGCTTGTCCTTCTTTGTGAAATGTCAAGAATGTCTATTTGAGTTTTCAATGAAGAAGCTTCTATGATGAGGGGGAAGAGTAAACTATCATTTAATAAATATGTCAGAACAATAAATACTGTGTGTTTAGTGGGTTGGAAAGGGATCCTAGTAATGTGTGGTCCCTTGCTAGATTTCATGTTTCCTTCTGAGCTTCGATTTCGAAAACCTTTTGTAACTATCCTATAGGTTCTATGTTCTATGGTTGGAGCCCCTTCATGTAGTTAGGCTCTTTTTTGTGGGCTGATTTTTTGTATGCCCATATAGTCTTTCATTTTATCTCAATGAAAGTTGTTTTAAACATCGATTATGTTTTTTACCGATGGGCTATTGTTGGAATTTGGCCTATATTGTCATTGTCCTGTGTGGTTTAGGTCCTGATTCTTTGGGGGTGATATTCAGGTGCCATGAAAATTTGATGTACGGTGGCTTAACATGGAGAACTGGATGCTGCCCATAAATTTTGTTTCAAATTGATACCTAGAAATGTTAACCAATGTGTAGTAGATTACTTAACCAAAGATGCATTCTTTCTGACCGTGACCACTATTAAGATGATAAAAATGAATGAGAAAAAGGGTTAAGAGAAGGGTGGTAGTGCTGAAAACATTTTATCCAATGCATTATTTGATCTAATCTGCTTGTAAGTAATGAAATTCTCATATTTGGTTTTCTCTTTTCAGTGTACACTCCAGAGCAGCTGGTGGAGAAATATGGAAATATAACTTTCCTTCTATACTGCGTAATTTTGATCTTGGTTGTTGCTTTACATCACTCAATCTATAGGTTAGAATTAAAATGGATTCTTTCCTTCGTACTTTATATGAGGCTTTGTGTTGATAATAGTATCCCCCATGGAACAAACATGCAGAAGGGGAGAACTGTTACTTTCTGTTTCTGGACAAGATCTCAGACCGTATTGGCATATGCTACTACCATTTTCCTATGCTGTAGTTTCAGGTGCAATAGGTTCTTGCTCGGTCTTGTTTGCCAAATCTCTGTAAGTTCGCTGACTATTTTGTCCTTTTTCCCTGTAGGTGCTGAACCCACTTATAAGTCTGTTTTGCTTTCTCTTTCTGATACCAAGTTATCTGTTTTAGCTCAATACTGCTCAGATTGGCCATGTCTAGTGATTATCAGCTGCATAGCTGGTTTACATACTCCTTACTGCTTTTATTTCTCAGTACTGCTGGATTTTGGGTAAGGTCATTATAGGACTCTGCTTTGCCTTCTTTCTTGGTTTCATATTCTTATTTCTAGTGATGTGAATAAACATCATTTCTTTAACTGTTATGCCCATTATGGATTTTTTTTTATCTATTTCCACAGATGGCCAGGTTAAACGAAGGACTTTCCCTGTTTGATGCAATCCTTATTGTTCCCATGTTTCAGATAGCTTGGACTTTCTTCTCCATATGTTCAGGTTTTATATACTTTAAAGAATTCCAGGTTCTCTCTCTCTCTCTCTCTCTCCTAAAGAATGTACTTTTTTGGGTGTTTTTTTTGGGGTGGGGTGAGGGTTCTTGAGCCTAAAGACATGAAGAGAACTGCATGAATGACATTGGTTAGGACCTACTGAAGCTACTGAATAATATGCCTATTCTTAGGAAAATGGTGTACTTAGCATTGGATCAAAGCTATGCCAATTGGCTTATACCTTAAATTGGAACTAGCATTTTTTCATACAAGATGTCTATAGCATTTTGTGGGCTGAGATGACTGTTCTCTGTTTATCACCTTTTTAACTCCCGAAGAACGAGATTCAGATTTCTTCTATGACTCGAGAAGTAACTAAGTTATGTTTAATTTTTCCCCTCTGCTTGACACAATTATTATGGCACCCACCATGTATTGGAAAATAGTCCTCCTGTTTCACTGTTTGTATAAAGTGGTAAAAAACGTGATAATTGTTTAACCATGTGCGTTTTTAACCTTTGATTTCAAGGTCATGCTTCCTGTTGCACTTTAACACTTTCCATGCCCTTATTATGAATTCAATTTAGGTAGCAAGCTTAGCTTTGTATTATCTTCTAAACAGGTGCTTGATGCTTTGAGGACGACTATGTTCATACTAGGAATGATGTCTGTTTTCATAGGCATTTCCTTGCTAGCGCCTGATGAGCCGAGAGGTATTTTAATTTAGGTTCAAAGTATTTTTGGGGATTATGTTACAAATCTGAGAGCTTGATTGAAACATGAATTTCAGACGGTGAATTCAAAGATAATTCATCTTTGGTTTCTGGGACGTCCACAAGCTTCGCATCAGAAGAAGAGAGGTACATCAATTATTTTGTATGCACATATGACATTAGTTTTTTTTCAAAATCTTACGGATTTTGAGTTCAGAACCACTTCTGTTTGCAATGAAGATATCTGGTATTGTACTCTTTTTTCTGGGGGCAGTGTTAGTCCTAAATTCGAATTTCTCAGCAGTGGGCAACCAAGGCTATTAAAAAGGTTTCCTCGTAGGGTTTAGTTTATGGAACATATAAAGTATTGGACAATGAAAAATCTCTGATGGATGAAGTTTGAAGTGCTTAGTCTTTCTGCCATTTGATCTGCAACTCCTTACATTTCTGTTGCATATGTTTCATTTTCAGACTAATTGTGTCATCCAAAGAATTAGAGACTAGGGACGCAAGATCGTTTTCACAAGGATTCTTGTTAAAGATTACAGATGTAGTAGCCAAGGCGAAGGTATTGGAGTCAGCAATGGTGATTTGGAAGCTTAGTTGCTCGATGGTCAACATGTTCAAACAGTAAATATATCCTGTGTTATTCTTTTTGCAGGCTGCTTTAGCACTTTCTCTGGGTTTTGGAGAAGATTCACTCAATGCATCTGCTATACTTGTGATGCCTATGGTGTCATCAAGGATGACTGGCTTTAGAGGAACTGGGTTTGAGCGCTCAAAGTTTTTCTCCATGAGGAAGCCTGATTGGAGTAAGATCTCATTGGATGAGGATACGAAGGTGCTCGACACAAACATAGGTTTCTCCCAAAACCTTTGAATAGGAGTAGTAGTAAGAACTTGGCTTGGAGTATTAAACCAACGCCTTTTCTCTATAGATATAGTAGTTAGAATTCAATATACCAGTTACTGAGCATGTTCTTACATAGAATTATAAAAAACAATATTGTATATTATGGAGAACAATTTTTTCTTATAAAGCAAGATATTATTTTTTTTCCCCCTTCA

mRNA sequence

ACGGACACGTCCACAAAAATTTAGTGGATCGATATTAAACTCATGATAGCACGGTCCCTCGTTATTAGTGTAGTCCGGAGCTTACCCGATCCATCTTGACCGACAGGTGACAGTTAAGTACGCCGGCCGCGGCCGTGGCTTTGTTCACAATCTTCACACACAGAATCACTCAGTATTCTCTCCCCTTCATCTCTAATTGACCGCACAACTTCCCTCTCTTTCACTTTTCTTCATCACGCTTTCCTTCCTCTCCGCCCTCTCACGACGGAGTCCGGCGGTTTTCTCTTCTCTCTACACTCCGAATTCCCTTGGGAGGGAAGCTACTCACACCCAATTCCTCCCTCCCATTCTTCCCACCTTCATTATATTTCTTCTCAATGCCCGACTTCCTCTCTTGGATTGGAACTTGATCTCAACTGCCCTCCATTCTCCACTGACGGATCTTCTCACAGGCTGGGATATTCAGCTGTATGCTGATTCGCTTATACCTCTCTAATTTATGGGGGAGTGGGTTATTGGAGCTGTCATCAACCTCTTTGGCAGCATTGCCATCAACTTTGGGACGAACCTTCTTAAATTAGGCCATAACGAGAGAGAGAAGCATTCTATGCTAGAAAACAATGGTTCTAATGGAAAGACTCCAATGAAGCCCATTATCTACTTCCAGACTTGGAGAGTTGGTATTACATTTTTCATTCTCGGAAATTGCCTTAATTTTATTTCCTTCGGATATGCTGCCCAGTCTCTTCTTGCGGCACTTGGATCAGTTCAGTTTGTGTCCAATATTGCTTTTGCATACTTTGTACTCCACAAGATGGTTACTGTTAAGGTAATGGTTGCCACTGCTTTTATTGTTCTTGGAAACGTTTTTCTAGTTGCTTTTGGCAACCATCAGTCCCCTGTGTACACTCCAGAGCAGCTGGTGGAGAAATATGGAAATATAACTTTCCTTCTATACTGCGTAATTTTGATCTTGGTTGTTGCTTTACATCACTCAATCTATAGAAGGGGAGAACTGTTACTTTCTGTTTCTGGACAAGATCTCAGACCGTATTGGCATATGCTACTACCATTTTCCTATGCTGTAGTTTCAGGTGCAATAGGTTCTTGCTCGGTCTTGTTTGCCAAATCTCTATTGGCCATGTCTAGTGATTATCAGCTGCATAGCTGGTTTACATACTCCTTACTGCTTTTATTTCTCAGTACTGCTGGATTTTGGGTGCTTGATGCTTTGAGGACGACTATGTTCATACTAGGAATGATGTCTGTTTTCATAGGCATTTCCTTGCTAGCGCCTGATGAGCCGAGAGACGGTGAATTCAAAGATAATTCATCTTTGGTTTCTGGGACGTCCACAAGCTTCGCATCAGAAGAAGAGAGTGTTAGTCCTAAATTCGAATTTCTCAGCAGTGGGCAACCAAGGCTATTAAAAAGACTAATTGTGTCATCCAAAGAATTAGAGACTAGGGACGCAAGATCGTTTTCACAAGGATTCTTGTTAAAGATTACAGATGTAGTAGCCAAGGCGAAGGCTGCTTTAGCACTTTCTCTGGGTTTTGGAGAAGATTCACTCAATGCATCTGCTATACTTGTGATGCCTATGGTGTCATCAAGGATGACTGGCTTTAGAGGAACTGGGTTTGAGCGCTCAAAGTTTTTCTCCATGAGGAAGCCTGATTGGAGTAAGATCTCATTGGATGAGGATACGAAGGTGCTCGACACAAACATAGGTTTCTCCCAAAACCTTTGAATAGGAGTAGTAGTAAGAACTTGGCTTGGAGTATTAAACCAACGCCTTTTCTCTATAGATATAGTAGTTAGAATTCAATATACCAGTTACTGAGCATGTTCTTACATAGAATTATAAAAAACAATATTGTATATTATGGAGAACAATTTTTTCTTATAAAGCAAGATATTATTTTTTTTCCCCCTTCA

Coding sequence (CDS)

ATGGGGGAGTGGGTTATTGGAGCTGTCATCAACCTCTTTGGCAGCATTGCCATCAACTTTGGGACGAACCTTCTTAAATTAGGCCATAACGAGAGAGAGAAGCATTCTATGCTAGAAAACAATGGTTCTAATGGAAAGACTCCAATGAAGCCCATTATCTACTTCCAGACTTGGAGAGTTGGTATTACATTTTTCATTCTCGGAAATTGCCTTAATTTTATTTCCTTCGGATATGCTGCCCAGTCTCTTCTTGCGGCACTTGGATCAGTTCAGTTTGTGTCCAATATTGCTTTTGCATACTTTGTACTCCACAAGATGGTTACTGTTAAGGTAATGGTTGCCACTGCTTTTATTGTTCTTGGAAACGTTTTTCTAGTTGCTTTTGGCAACCATCAGTCCCCTGTGTACACTCCAGAGCAGCTGGTGGAGAAATATGGAAATATAACTTTCCTTCTATACTGCGTAATTTTGATCTTGGTTGTTGCTTTACATCACTCAATCTATAGAAGGGGAGAACTGTTACTTTCTGTTTCTGGACAAGATCTCAGACCGTATTGGCATATGCTACTACCATTTTCCTATGCTGTAGTTTCAGGTGCAATAGGTTCTTGCTCGGTCTTGTTTGCCAAATCTCTATTGGCCATGTCTAGTGATTATCAGCTGCATAGCTGGTTTACATACTCCTTACTGCTTTTATTTCTCAGTACTGCTGGATTTTGGGTGCTTGATGCTTTGAGGACGACTATGTTCATACTAGGAATGATGTCTGTTTTCATAGGCATTTCCTTGCTAGCGCCTGATGAGCCGAGAGACGGTGAATTCAAAGATAATTCATCTTTGGTTTCTGGGACGTCCACAAGCTTCGCATCAGAAGAAGAGAGTGTTAGTCCTAAATTCGAATTTCTCAGCAGTGGGCAACCAAGGCTATTAAAAAGACTAATTGTGTCATCCAAAGAATTAGAGACTAGGGACGCAAGATCGTTTTCACAAGGATTCTTGTTAAAGATTACAGATGTAGTAGCCAAGGCGAAGGCTGCTTTAGCACTTTCTCTGGGTTTTGGAGAAGATTCACTCAATGCATCTGCTATACTTGTGATGCCTATGGTGTCATCAAGGATGACTGGCTTTAGAGGAACTGGGTTTGAGCGCTCAAAGTTTTTCTCCATGAGGAAGCCTGATTGGAGTAAGATCTCATTGGATGAGGATACGAAGGTGCTCGACACAAACATAGGTTTCTCCCAAAACCTTTGA

Protein sequence

MGEWVIGAVINLFGSIAINFGTNLLKLGHNEREKHSMLENNGSNGKTPMKPIIYFQTWRVGITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVLGNVFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILVVALHHSIYRRGELLLSVSGQDLRPYWHMLLPFSYAVVSGAIGSCSVLFAKSLLAMSSDYQLHSWFTYSLLLLFLSTAGFWVLDALRTTMFILGMMSVFIGISLLAPDEPRDGEFKDNSSLVSGTSTSFASEEESVSPKFEFLSSGQPRLLKRLIVSSKELETRDARSFSQGFLLKITDVVAKAKAALALSLGFGEDSLNASAILVMPMVSSRMTGFRGTGFERSKFFSMRKPDWSKISLDEDTKVLDTNIGFSQNL
BLAST of Lsi05G004280 vs. Swiss-Prot
Match: NIPA8_ARATH (Probable magnesium transporter NIPA8 OS=Arabidopsis thaliana GN=At3g26670 PE=2 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 1.5e-103
Identity = 193/246 (78.46%), Postives = 218/246 (88.62%), Query Frame = 1

Query: 1   MGEWVIGAVINLFGSIAINFGTNLLKLGHNEREKHSMLENNGSNGKTPMKPIIYFQTWRV 60
           MGEWVIGA IN+FGS+AINFGTNLLKLGHNERE+ ++ +     GK P+KPII+ QTWRV
Sbjct: 1   MGEWVIGAFINIFGSVAINFGTNLLKLGHNERERLALQD---GGGKMPLKPIIHNQTWRV 60

Query: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120
           GI  F+LGNCLNFISFGYAAQSLLAALGS+QFVSNIAFAY VL+KMVTVKV+VATAFIVL
Sbjct: 61  GILVFLLGNCLNFISFGYAAQSLLAALGSIQFVSNIAFAYVVLNKMVTVKVLVATAFIVL 120

Query: 121 GNVFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILVVALHHSIYRRGELLLSVSGQ 180
           GNVFLVAFGNHQSPV+TPEQL EKY N+TFL+YC ILIL+VA+HH +YR+GE+L+S  GQ
Sbjct: 121 GNVFLVAFGNHQSPVFTPEQLAEKYSNVTFLVYCGILILIVAVHHFLYRKGEVLISTPGQ 180

Query: 181 DLRPYWHMLLPFSYAVVSGAIGSCSVLFAKSL-----LAMSSDYQLHSWFTYSLLLLFLS 240
           ++  YW MLLPFSYAVVSGAIGSCSVLFAKSL     LAMSS YQLHSWFTYS+LLLFLS
Sbjct: 181 EISSYWKMLLPFSYAVVSGAIGSCSVLFAKSLSNLLRLAMSSSYQLHSWFTYSMLLLFLS 240

Query: 241 TAGFWV 242
           TAGFW+
Sbjct: 241 TAGFWM 243

BLAST of Lsi05G004280 vs. TrEMBL
Match: A0A0D2S8G3_GOSRA (Probable magnesium transporter OS=Gossypium raimondii GN=B456_007G071300 PE=3 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 5.7e-155
Identity = 294/448 (65.62%), Postives = 345/448 (77.01%), Query Frame = 1

Query: 1   MGEWVIGAVINLFGSIAINFGTNLLKLGHNEREKHSMLENNGSNGKTPMKPIIYFQTWRV 60
           MG+WVIGA INLFGSIAINFGTNLLKLGHNERE+HSM++  G  GK P+KPIIYFQTWRV
Sbjct: 1   MGDWVIGAFINLFGSIAINFGTNLLKLGHNERERHSMMDGEGMAGKVPLKPIIYFQTWRV 60

Query: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120
           GI FFI+GNCLNFISFGYAAQSLLAALGSVQF+SNIAFAYFVL+KMVTVKV+ ATAFIVL
Sbjct: 61  GILFFIIGNCLNFISFGYAAQSLLAALGSVQFISNIAFAYFVLNKMVTVKVLAATAFIVL 120

Query: 121 GNVFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILVVALHHSIYRRGELLLSVSGQ 180
           GN FLVAFGNHQSPVYTPEQL EKY NITFLLYC+ILILVVALHH IYRRGE++L+VSGQ
Sbjct: 121 GNFFLVAFGNHQSPVYTPEQLAEKYSNITFLLYCLILILVVALHHYIYRRGEIVLAVSGQ 180

Query: 181 DLRPYWHMLLPFSYAVVSGAIGSCSVLFAKSL-----LAMSSDYQLHSWFTYSLLLLFLS 240
           DLRPYW+MLLPFSYAVVSGA+GSCSVLFAKSL     LAMS+ YQLHSWFTYS++LLFLS
Sbjct: 181 DLRPYWNMLLPFSYAVVSGAVGSCSVLFAKSLSNLLRLAMSNGYQLHSWFTYSMILLFLS 240

Query: 241 TAGFW----------------------------------------VLDALRTTMFILGMM 300
           TAGFW                                        V DALRTTMFILGM+
Sbjct: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSIFTGFIYFQEYQVFDALRTTMFILGMV 300

Query: 301 SVFIGISLLAPDEPRDGEFKDNSSLVSGTSTSFASEEESVSPKFEFLSSGQPRLLKRLIV 360
           SVF GISLLAPDEPR GE KD S   S  S+S ++E +                  R I 
Sbjct: 301 SVFAGISLLAPDEPRGGEVKDGSHPTSVLSSSNSTETD------------------RFIF 360

Query: 361 SSKELETRDARSFSQGFLLKITDVVAKAKAALALSLGFGEDSLNASAILVMPMVSSRMTG 404
            S++ E ++ +SF+Q  ++K+TD++AKAK   +LSLGFG+DS+NASA+LVMPM+SS++TG
Sbjct: 361 ESEDAENKEMKSFTQLMVVKLTDILAKAKTTCSLSLGFGDDSINASAVLVMPMMSSKITG 420

BLAST of Lsi05G004280 vs. TrEMBL
Match: A0A0A0L9M2_CUCSA (Probable magnesium transporter OS=Cucumis sativus GN=Csa_3G251940 PE=3 SV=1)

HSP 1 Score: 531.9 bits (1369), Expect = 6.7e-148
Identity = 292/349 (83.67%), Postives = 299/349 (85.67%), Query Frame = 1

Query: 1   MGEWVIGAVINLFGSIAINFGTNLLKLGHNEREKHSMLENNGSNGKTPMKPIIYFQTWRV 60
           MGEWVIGAVINLFGS+AINFGTNLLKLGHNEREKHSMLENNGS GKTPMKPIIYFQTWRV
Sbjct: 1   MGEWVIGAVINLFGSLAINFGTNLLKLGHNEREKHSMLENNGSIGKTPMKPIIYFQTWRV 60

Query: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120
           GITFFI+GNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL
Sbjct: 61  GITFFIIGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120

Query: 121 GNVFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILVVALHHSIYRRGELLLSVSGQ 180
           GNVFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILVVALHHSIYRRGELLLSVSGQ
Sbjct: 121 GNVFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILVVALHHSIYRRGELLLSVSGQ 180

Query: 181 DLRPYWHMLLPFSYAVVSGAIGSCSVLFAKSL-----LAMSSDYQLHSWFTYSLLLLFLS 240
           DLRPYWHMLLPFSYA+VSGAIGSCSVLFAKSL     LAMSSDYQLHSWFTYSLLLLFLS
Sbjct: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS 240

Query: 241 TAGFWVLDALRTTMFILGMMSVFIGISLLAPDEPRDGEFKDNSSLVSGTSTSFASEEESV 300
           TAGFW+   LR      G ++              DGEFKDNSSLVSGTSTSF SEEE  
Sbjct: 241 TAGFWIRFRLR-----FGKIT-----------NRGDGEFKDNSSLVSGTSTSFTSEEE-- 300

Query: 301 SPKFEFLSSGQPRLLKRLIVSSKELETRDARSFSQGFLLKITDVVAKAK 345
                           RLIVSSKELETRDA+SFSQG LLKITDVVAKAK
Sbjct: 301 ----------------RLIVSSKELETRDAKSFSQGVLLKITDVVAKAK 315

BLAST of Lsi05G004280 vs. TrEMBL
Match: A0A0B0MPV4_GOSAR (Probable magnesium transporter OS=Gossypium arboreum GN=F383_29379 PE=3 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 2.2e-146
Identity = 284/448 (63.39%), Postives = 338/448 (75.45%), Query Frame = 1

Query: 1   MGEWVIGAVINLFGSIAINFGTNLLKLGHNEREKHSMLENNGSNGKTPMKPIIYFQTWRV 60
           MG+WVIGA INLFGSIAINFGTNLLKLGHNERE+HSM++  G  GK P+KPIIYFQTWRV
Sbjct: 1   MGDWVIGAFINLFGSIAINFGTNLLKLGHNERERHSMMDGEGMAGKVPLKPIIYFQTWRV 60

Query: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120
           GI FFI+GNCLNFISFGYAAQSLLAALGSVQF+SNIAFAYFVL+KMVTVKV+ ATAFIVL
Sbjct: 61  GILFFIIGNCLNFISFGYAAQSLLAALGSVQFISNIAFAYFVLNKMVTVKVLAATAFIVL 120

Query: 121 GNVFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILVVALHHSIYRRGELLLSVSGQ 180
           GN FLVAFGNHQSPVYTPEQL EKY NITFLLYC+ILILVVALHH IYRRGE++LSVSGQ
Sbjct: 121 GNFFLVAFGNHQSPVYTPEQLAEKYSNITFLLYCLILILVVALHHYIYRRGEIVLSVSGQ 180

Query: 181 DLRPYWHMLLPFSYAVVSGAIGSCSVLFAKSL-----LAMSSDYQLHSWFTYSLLLLFLS 240
           DLRPYW+MLLPFSYAVVSGA+GSCSVLFAKSL     LAMS+ YQLHSWFTYS++LLFLS
Sbjct: 181 DLRPYWNMLLPFSYAVVSGAVGSCSVLFAKSLSNLLRLAMSNGYQLHSWFTYSMILLFLS 240

Query: 241 TAGFWVLD-----ALRTTMFILGMMSV---------------------------FI---- 300
           TAGFW+       +L   + I+ M  +                           FI    
Sbjct: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFQEYQVFDALRTTMFILGMV 300

Query: 301 ----GISLLAPDEPRDGEFKDNSSLVSGTSTSFASEEESVSPKFEFLSSGQPRLLKRLIV 360
               GISLLAPDEPR GE KD S   S  S+S ++E +                  R I 
Sbjct: 301 SVFAGISLLAPDEPRGGEVKDASHPTSVLSSSNSTETD------------------RFIF 360

Query: 361 SSKELETRDARSFSQGFLLKITDVVAKAKAALALSLGFGEDSLNASAILVMPMVSSRMTG 404
            S++ E ++ +SF+Q  ++K+TD++AKAK   +LSLGFG+DS+NASA+LVMPM+SS++TG
Sbjct: 361 ESEDAENKEMKSFTQRMVVKVTDILAKAKTTCSLSLGFGDDSINASAVLVMPMMSSKITG 420

BLAST of Lsi05G004280 vs. TrEMBL
Match: A0A061EF19_THECC (Probable magnesium transporter OS=Theobroma cacao GN=TCM_017396 PE=3 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 4.8e-146
Identity = 287/448 (64.06%), Postives = 340/448 (75.89%), Query Frame = 1

Query: 1   MGEWVIGAVINLFGSIAINFGTNLLKLGHNEREKHSMLENNGSNGKTPMKPIIYFQTWRV 60
           MGEWVIGA INLFGSIAINFGTNLLKLGHNERE+HS ++     GKTP+KPIIYFQTWRV
Sbjct: 1   MGEWVIGAFINLFGSIAINFGTNLLKLGHNERERHSTMD---GGGKTPLKPIIYFQTWRV 60

Query: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120
           GI FF+LGNCLNFISFGYAAQSLLAALGSVQFVSNIAFA+FVL+KMVTVKV+VATAFIVL
Sbjct: 61  GILFFVLGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAFFVLNKMVTVKVLVATAFIVL 120

Query: 121 GNVFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILVVALHHSIYRRGELLLSVSGQ 180
           GN+FLVAFGNHQSPVYTPEQL EKY NITFLLYC+ILILVVALHHSIYRRGE LL+VSGQ
Sbjct: 121 GNIFLVAFGNHQSPVYTPEQLAEKYSNITFLLYCLILILVVALHHSIYRRGEHLLAVSGQ 180

Query: 181 DLRPYWHMLLPFSYAVVSGAIGSCSVLFAKSL-----LAMSSDYQLHSWFTYSLLLLFLS 240
           DLRPYW+MLLPFSYAVVSGA+GSCSVLFAKSL     LAMS+ YQLHSWFTYS+LLLFLS
Sbjct: 181 DLRPYWNMLLPFSYAVVSGAVGSCSVLFAKSLSNLLRLAMSNGYQLHSWFTYSMLLLFLS 240

Query: 241 TAGFWVLD-----ALRTTMFILGMMSV---------------------------FI---- 300
           TAGFW+       +L   + I+ M  +                           FI    
Sbjct: 241 TAGFWMTRLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFQEYQVFDALRTTMFILGMM 300

Query: 301 ----GISLLAPDEPRDGEFKDNSSLVSGTSTSFASEEESVSPKFEFLSSGQPRLLKRLIV 360
               GISLLAPDE R GE KD  S++S ++++                        RLI+
Sbjct: 301 SVFIGISLLAPDESRGGEVKDIDSVMSSSNSTETD---------------------RLIL 360

Query: 361 SSKELETRDARSFSQGFLLKITDVVAKAKAALALSLGFGEDSLNASAILVMPMVSSRMTG 404
            S++++ ++ +SF+QG ++KITD++AKAK   +LSLGFGEDS+NASA+LVMPM+SS++TG
Sbjct: 361 PSEDVQNKEIKSFTQGMMMKITDMLAKAKMTCSLSLGFGEDSINASAVLVMPMMSSKITG 420

BLAST of Lsi05G004280 vs. TrEMBL
Match: W9QUK6_9ROSA (Probable magnesium transporter OS=Morus notabilis GN=L484_007572 PE=3 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 8.2e-146
Identity = 296/457 (64.77%), Postives = 342/457 (74.84%), Query Frame = 1

Query: 1   MGEWVIGAVINLFGSIAINFGTNLLKLGHNEREKHSMLENNG-SNGKTPMKPIIYFQTWR 60
           MGEWV+GA INLFGSIAINFGTNLLKLGHNERE+H+ LE++G SNGK  +KPIIYFQTWR
Sbjct: 5   MGEWVVGAFINLFGSIAINFGTNLLKLGHNERERHASLESDGTSNGKHTLKPIIYFQTWR 64

Query: 61  VGITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIV 120
           VGI FFILGNCLNFISFGYAAQSLLAALGS+QFVSN+AFAYFVL+K VTV VMVATAFIV
Sbjct: 65  VGIVFFILGNCLNFISFGYAAQSLLAALGSIQFVSNMAFAYFVLNKRVTVNVMVATAFIV 124

Query: 121 LGNVFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILVVALHHSIYRRGELLLSVSG 180
           LGNVFLVAFGNHQSPVYTPEQL EKY N+TFL YCVILILVVA+HHSIYRRGELLL+VSG
Sbjct: 125 LGNVFLVAFGNHQSPVYTPEQLAEKYSNVTFLFYCVILILVVAVHHSIYRRGELLLAVSG 184

Query: 181 QDLRPYWHMLLPFSYAVVSGAIGSCSVLFAKSL-----LAMSSDYQLHSWFTYSLLLLFL 240
            DLRPYWHMLLPFSYA+VSGA+GSCSVLFAKSL     LAMSS YQLHSWFTYS+LLLFL
Sbjct: 185 HDLRPYWHMLLPFSYAIVSGAVGSCSVLFAKSLSNLLRLAMSSSYQLHSWFTYSMLLLFL 244

Query: 241 STAGFWVLD-----ALRTTMFILGMMSV---------------------------FI--- 300
           STAGFW+       +L   + I+ M  +                           FI   
Sbjct: 245 STAGFWMTRLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFQEYQVFDALRTTMFILGM 304

Query: 301 -----GISLLAPDEPRDGEFKDNSSLVSGTSTSFASEEESVSPKFEFLSSGQPRLLKRLI 360
                GISLLAPDE +  E KDN+SLVS                   +SS  P+ + RL+
Sbjct: 305 MSVFIGISLLAPDESKGSEVKDNTSLVS------------------VMSSSIPKEVDRLV 364

Query: 361 VSSKELETRDARSFSQGFLLKITDVVAKAKAALALSLGFGEDSLNASAILVMPMVSSRMT 406
           V S++  +RD+ SF QG ++K++D+V KAK A ALSLGFGEDS+NASA+LVMPMVSS++T
Sbjct: 365 VGSEDAISRDSGSFMQGLVMKMSDIVVKAKNACALSLGFGEDSINASAVLVMPMVSSKIT 424

BLAST of Lsi05G004280 vs. TAIR10
Match: AT3G26670.1 (AT3G26670.1 Protein of unknown function (DUF803))

HSP 1 Score: 377.9 bits (969), Expect = 8.2e-105
Identity = 193/246 (78.46%), Postives = 218/246 (88.62%), Query Frame = 1

Query: 1   MGEWVIGAVINLFGSIAINFGTNLLKLGHNEREKHSMLENNGSNGKTPMKPIIYFQTWRV 60
           MGEWVIGA IN+FGS+AINFGTNLLKLGHNERE+ ++ +     GK P+KPII+ QTWRV
Sbjct: 1   MGEWVIGAFINIFGSVAINFGTNLLKLGHNERERLALQD---GGGKMPLKPIIHNQTWRV 60

Query: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120
           GI  F+LGNCLNFISFGYAAQSLLAALGS+QFVSNIAFAY VL+KMVTVKV+VATAFIVL
Sbjct: 61  GILVFLLGNCLNFISFGYAAQSLLAALGSIQFVSNIAFAYVVLNKMVTVKVLVATAFIVL 120

Query: 121 GNVFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILVVALHHSIYRRGELLLSVSGQ 180
           GNVFLVAFGNHQSPV+TPEQL EKY N+TFL+YC ILIL+VA+HH +YR+GE+L+S  GQ
Sbjct: 121 GNVFLVAFGNHQSPVFTPEQLAEKYSNVTFLVYCGILILIVAVHHFLYRKGEVLISTPGQ 180

Query: 181 DLRPYWHMLLPFSYAVVSGAIGSCSVLFAKSL-----LAMSSDYQLHSWFTYSLLLLFLS 240
           ++  YW MLLPFSYAVVSGAIGSCSVLFAKSL     LAMSS YQLHSWFTYS+LLLFLS
Sbjct: 181 EISSYWKMLLPFSYAVVSGAIGSCSVLFAKSLSNLLRLAMSSSYQLHSWFTYSMLLLFLS 240

Query: 241 TAGFWV 242
           TAGFW+
Sbjct: 241 TAGFWM 243

BLAST of Lsi05G004280 vs. NCBI nr
Match: gi|778680509|ref|XP_004145621.2| (PREDICTED: probable magnesium transporter NIPA8 [Cucumis sativus])

HSP 1 Score: 621.7 bits (1602), Expect = 9.3e-175
Identity = 353/452 (78.10%), Postives = 368/452 (81.42%), Query Frame = 1

Query: 1   MGEWVIGAVINLFGSIAINFGTNLLKLGHNEREKHSMLENNGSNGKTPMKPIIYFQTWRV 60
           MGEWVIGAVINLFGS+AINFGTNLLKLGHNEREKHSMLENNGS GKTPMKPIIYFQTWRV
Sbjct: 1   MGEWVIGAVINLFGSLAINFGTNLLKLGHNEREKHSMLENNGSIGKTPMKPIIYFQTWRV 60

Query: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120
           GITFFI+GNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL
Sbjct: 61  GITFFIIGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120

Query: 121 GNVFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILVVALHHSIYRRGELLLSVSGQ 180
           GNVFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILVVALHHSIYRRGELLLSVSGQ
Sbjct: 121 GNVFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILVVALHHSIYRRGELLLSVSGQ 180

Query: 181 DLRPYWHMLLPFSYAVVSGAIGSCSVLFAKSL-----LAMSSDYQLHSWFTYSLLLLFLS 240
           DLRPYWHMLLPFSYA+VSGAIGSCSVLFAKSL     LAMSSDYQLHSWFTYSLLLLFLS
Sbjct: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS 240

Query: 241 TAGFW---------VLDA-LRTTMFILGMMSVFIGISLLAPDEPRDGEFKDNSSLVSGTS 300
           TAGFW         + DA L   MF +      I    +         FK+   L +  +
Sbjct: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFI--------YFKEFQVLDALRT 300

Query: 301 TSFASEEESVSPKFEFLSSGQPR--------------------LLKRLIVSSKELETRDA 360
           T F     SV      L+  +PR                      +RLIVSSKELETRDA
Sbjct: 301 TMFILGMMSVFIGISLLAPDEPRDGEFKDNSSLVSGTSTSFTSEEERLIVSSKELETRDA 360

Query: 361 RSFSQGFLLKITDVVAKAKAALALSLGFGEDSLNASAILVMPMVSSRMTGFRGTGFERSK 417
           +SFSQG LLKITDVVAKAKAALALSLGFGEDSLNASAILVMPMVSSRMTGFRGTGFERSK
Sbjct: 361 KSFSQGVLLKITDVVAKAKAALALSLGFGEDSLNASAILVMPMVSSRMTGFRGTGFERSK 420

BLAST of Lsi05G004280 vs. NCBI nr
Match: gi|659098098|ref|XP_008449977.1| (PREDICTED: uncharacterized protein LOC103491699 [Cucumis melo])

HSP 1 Score: 619.4 bits (1596), Expect = 4.6e-174
Identity = 351/452 (77.65%), Postives = 367/452 (81.19%), Query Frame = 1

Query: 1   MGEWVIGAVINLFGSIAINFGTNLLKLGHNEREKHSMLENNGSNGKTPMKPIIYFQTWRV 60
           MGEWVIGAVINLFGS+AINFGTNLLKLGHNEREKHSMLENNGS GKTPMKPIIYFQTWRV
Sbjct: 1   MGEWVIGAVINLFGSLAINFGTNLLKLGHNEREKHSMLENNGSIGKTPMKPIIYFQTWRV 60

Query: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120
           GITFFI+GNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL
Sbjct: 61  GITFFIIGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120

Query: 121 GNVFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILVVALHHSIYRRGELLLSVSGQ 180
           GNVFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILI+VVALHHSIYRRGELLLSVSGQ
Sbjct: 121 GNVFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILIVVVALHHSIYRRGELLLSVSGQ 180

Query: 181 DLRPYWHMLLPFSYAVVSGAIGSCSVLFAKSL-----LAMSSDYQLHSWFTYSLLLLFLS 240
           DLR YWHMLLPFSYA+VSGAIGSCSVLFAKSL     LAMSSDYQLHSWFTYSLLLLFLS
Sbjct: 181 DLRSYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS 240

Query: 241 TAGFW---------VLDA-LRTTMFILGMMSVFIGISLLAPDEPRDGEFKDNSSLVSGTS 300
           TAGFW         + DA L   MF +      I    +         FK+   L +  +
Sbjct: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFI--------YFKEFQVLDALRT 300

Query: 301 TSFASEEESVSPKFEFLSSGQPR--------------------LLKRLIVSSKELETRDA 360
           T F     SV      L+  +PR                      +RLIVSSKE ETRDA
Sbjct: 301 TMFILGMMSVFIGISLLAPDEPRDGEFKDNSSLVSGTSTSFTSEEERLIVSSKESETRDA 360

Query: 361 RSFSQGFLLKITDVVAKAKAALALSLGFGEDSLNASAILVMPMVSSRMTGFRGTGFERSK 417
           +SFSQGFLLKITDVVAKAKAALALSLGFGEDSLNASA+LVMPMVSSRMTGFRGTGFERSK
Sbjct: 361 KSFSQGFLLKITDVVAKAKAALALSLGFGEDSLNASAVLVMPMVSSRMTGFRGTGFERSK 420

BLAST of Lsi05G004280 vs. NCBI nr
Match: gi|694436065|ref|XP_009345158.1| (PREDICTED: uncharacterized protein LOC103936992 [Pyrus x bretschneideri])

HSP 1 Score: 576.6 bits (1485), Expect = 3.4e-161
Identity = 307/455 (67.47%), Postives = 356/455 (78.24%), Query Frame = 1

Query: 1   MGEWVIGAVINLFGSIAINFGTNLLKLGHNEREKHSMLENNGSNGKTPMKPIIYFQTWRV 60
           MGEWV+GA INLFGSIAINFGTNLLKLGHNERE+HS+LE++G NGK P+KPII +QTWR+
Sbjct: 1   MGEWVVGAFINLFGSIAINFGTNLLKLGHNERERHSILESDGGNGKLPLKPIIQYQTWRI 60

Query: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120
           G  FF LGNCLNF+SFGYAAQSLLAALGSVQFVSNIAFAYFVL+KMVTVKVMVATAFIVL
Sbjct: 61  GFLFFALGNCLNFVSFGYAAQSLLAALGSVQFVSNIAFAYFVLNKMVTVKVMVATAFIVL 120

Query: 121 GNVFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILVVALHHSIYRRGELLLSVSGQ 180
           GN+FLVAFGNHQSPVYTP+QL EKY NI FLLYC+IL+LVVALHHSIYRRGELL +VSGQ
Sbjct: 121 GNIFLVAFGNHQSPVYTPQQLAEKYSNIAFLLYCMILVLVVALHHSIYRRGELLHAVSGQ 180

Query: 181 DLRPYWHMLLPFSYAVVSGAIGSCSVLFAKSL-----LAMSSDYQLHSWFTYSLLLLFLS 240
           DLRPYWHMLLPFSYA+VSGA+GS SVLFAKSL     LA+SS YQ+HSWFTYS+LLLFLS
Sbjct: 181 DLRPYWHMLLPFSYAIVSGAVGSFSVLFAKSLSNLLRLALSSSYQIHSWFTYSMLLLFLS 240

Query: 241 TAGFW----------------------------------------VLDALRTTMFILGMM 300
           TAGFW                                        VLDALRTTMFILGMM
Sbjct: 241 TAGFWMARLNEGLSFFDAILIVPMFQIAWTFFSICTGFVYFQEYQVLDALRTTMFILGMM 300

Query: 301 SVFIGISLLAPDEPRDGEFKDNSSLVSGTSTSFASEEESVSPKFEFLSSGQPRLLKRLIV 360
           SVF+GI+LLAPD+ +  E KDN SLVS  S+S + +                  + RL +
Sbjct: 301 SVFVGITLLAPDDSKGAEVKDNPSLVSVVSSSISKD------------------VGRLAI 360

Query: 361 SSKELETRDARSFSQGFLLKITDVVAKAKAALALSLGFGEDSLNASAILVMPMVSSRMTG 410
           SS++++TRDA+SF QG L+KI+D + KAK A AL+LGFGEDS+NASA+LVMPMVSS++TG
Sbjct: 361 SSEDVQTRDAKSFVQGILVKISDAIVKAKTACALALGFGEDSINASAVLVMPMVSSKITG 420

BLAST of Lsi05G004280 vs. NCBI nr
Match: gi|694406561|ref|XP_009378082.1| (PREDICTED: uncharacterized protein LOC103966622 [Pyrus x bretschneideri])

HSP 1 Score: 573.5 bits (1477), Expect = 2.9e-160
Identity = 308/461 (66.81%), Postives = 354/461 (76.79%), Query Frame = 1

Query: 1   MGEWVIGAVINLFGSIAINFGTNLLKLGHNEREKHSMLENNGSNGKTPMKPIIYFQTWRV 60
           MGEWV+GA INLFGSIAINFGTNLLKLGHNERE+HS+LE++G NGK P+KPII++QTWRV
Sbjct: 1   MGEWVVGAFINLFGSIAINFGTNLLKLGHNERERHSILESDGGNGKLPLKPIIHYQTWRV 60

Query: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120
           G  FF LGNCLNF+SFGYAAQSLLAALGSVQFVSNIAFAYFVL+KMVTVKVMVATAFIV+
Sbjct: 61  GFLFFALGNCLNFVSFGYAAQSLLAALGSVQFVSNIAFAYFVLNKMVTVKVMVATAFIVI 120

Query: 121 GNVFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILVVALHHSIYRRGELLLSVSGQ 180
           GN+FLVAFGNHQSPVYTPEQL EKY NI FL YC+IL+LVVALHHSIYRRGELL +VSGQ
Sbjct: 121 GNIFLVAFGNHQSPVYTPEQLAEKYSNIAFLFYCMILVLVVALHHSIYRRGELLHAVSGQ 180

Query: 181 DLRPYWHMLLPFSYAVVSGAIGSCSVLFAKSL-----LAMSSDYQLHSWFTYSLLLLFLS 240
           DLRPYWHMLLPFSYA+VSGA+GS SVLFAKSL     LA+SS YQLHSWFTYS+LLLFLS
Sbjct: 181 DLRPYWHMLLPFSYAIVSGAVGSFSVLFAKSLSNLLRLAISSSYQLHSWFTYSMLLLFLS 240

Query: 241 TAGFW----------------------------------------VLDALRTTMFILGMM 300
           TAGFW                                        VLDALRTTMFILGMM
Sbjct: 241 TAGFWMARLNEGLSFFDAILIVPMFQIAWTFFSICTGFVYFQEYQVLDALRTTMFILGMM 300

Query: 301 SVFIGISLLAPDEPRDGEFKDNSSLVSGTSTSFASEEESVSPKFEFLSSGQPRLLKRLIV 360
           SVF+GI+LLAPD+ R  E K+N SLVS  S+S                   P+ + R  V
Sbjct: 301 SVFVGITLLAPDDSRGAEVKENPSLVSVVSSSI------------------PKDMGRFAV 360

Query: 361 SSKELETRDARSFSQGFLLKITDVVAKAKAALALSLGFGEDSLNASAILVMPMVSSRMTG 416
           SS++++ RD RSF QG L+KI+D + KAK A ALSLGFG+DS+NASA+LVMPMVSS++TG
Sbjct: 361 SSEDVQIRDTRSFVQGILVKISDAIVKAKTACALSLGFGDDSINASAVLVMPMVSSKITG 420

BLAST of Lsi05G004280 vs. NCBI nr
Match: gi|657974404|ref|XP_008379030.1| (PREDICTED: uncharacterized protein LOC103442068 [Malus domestica])

HSP 1 Score: 571.2 bits (1471), Expect = 1.4e-159
Identity = 306/455 (67.25%), Postives = 353/455 (77.58%), Query Frame = 1

Query: 1   MGEWVIGAVINLFGSIAINFGTNLLKLGHNEREKHSMLENNGSNGKTPMKPIIYFQTWRV 60
           MGEWV+GA INLFGSIAINFGTN LKLGHNERE+HS+LE++G NGK P+KPII +QTWR+
Sbjct: 1   MGEWVVGAFINLFGSIAINFGTNXLKLGHNERERHSILESDGGNGKLPLKPIIQYQTWRI 60

Query: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120
           G  FF LGNCLNF+SFGYAAQSLLAALGSVQFVSNIAFAYFVL+KMVTVKVMVATAFIVL
Sbjct: 61  GFLFFALGNCLNFVSFGYAAQSLLAALGSVQFVSNIAFAYFVLNKMVTVKVMVATAFIVL 120

Query: 121 GNVFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILVVALHHSIYRRGELLLSVSGQ 180
           GN+FLVAFGNHQSPVYTPEQL EKY NI FL YC+IL+LVVALHHSIYRRGELL +VSGQ
Sbjct: 121 GNIFLVAFGNHQSPVYTPEQLAEKYSNIAFLFYCMILVLVVALHHSIYRRGELLHAVSGQ 180

Query: 181 DLRPYWHMLLPFSYAVVSGAIGSCSVLFAKSL-----LAMSSDYQLHSWFTYSLLLLFLS 240
            LRPYWHMLLPFSYA+VSGA+GS SVLFAKSL     LA+SS YQLHSWFTYS+LLLFLS
Sbjct: 181 XLRPYWHMLLPFSYAIVSGAVGSFSVLFAKSLSNLLRLALSSSYQLHSWFTYSMLLLFLS 240

Query: 241 TAGFW----------------------------------------VLDALRTTMFILGMM 300
           TAGFW                                        VLDALRTTMFILGM+
Sbjct: 241 TAGFWMARLNEGLSFFDAILIVPMFQIAWTFFSICTGFVYFQEYQVLDALRTTMFILGMI 300

Query: 301 SVFIGISLLAPDEPRDGEFKDNSSLVSGTSTSFASEEESVSPKFEFLSSGQPRLLKRLIV 360
           SVF+GI+LLAPD+ +  E KDN SLVS  S+S + +                  + RL V
Sbjct: 301 SVFVGITLLAPDDSKGAEVKDNPSLVSVVSSSISKD------------------VGRLAV 360

Query: 361 SSKELETRDARSFSQGFLLKITDVVAKAKAALALSLGFGEDSLNASAILVMPMVSSRMTG 410
           SS++++TRDA+SF QG L+KI+D + KAK A AL+LGFGEDS+NASA+LVMPMVSS++TG
Sbjct: 361 SSEDVQTRDAKSFVQGILVKISDAIVKAKTACALALGFGEDSINASAVLVMPMVSSKITG 420

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
NIPA8_ARATH1.5e-10378.46Probable magnesium transporter NIPA8 OS=Arabidopsis thaliana GN=At3g26670 PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A0D2S8G3_GOSRA5.7e-15565.63Probable magnesium transporter OS=Gossypium raimondii GN=B456_007G071300 PE=3 SV... [more]
A0A0A0L9M2_CUCSA6.7e-14883.67Probable magnesium transporter OS=Cucumis sativus GN=Csa_3G251940 PE=3 SV=1[more]
A0A0B0MPV4_GOSAR2.2e-14663.39Probable magnesium transporter OS=Gossypium arboreum GN=F383_29379 PE=3 SV=1[more]
A0A061EF19_THECC4.8e-14664.06Probable magnesium transporter OS=Theobroma cacao GN=TCM_017396 PE=3 SV=1[more]
W9QUK6_9ROSA8.2e-14664.77Probable magnesium transporter OS=Morus notabilis GN=L484_007572 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G26670.18.2e-10578.46 Protein of unknown function (DUF803)[more]
Match NameE-valueIdentityDescription
gi|778680509|ref|XP_004145621.2|9.3e-17578.10PREDICTED: probable magnesium transporter NIPA8 [Cucumis sativus][more]
gi|659098098|ref|XP_008449977.1|4.6e-17477.65PREDICTED: uncharacterized protein LOC103491699 [Cucumis melo][more]
gi|694436065|ref|XP_009345158.1|3.4e-16167.47PREDICTED: uncharacterized protein LOC103936992 [Pyrus x bretschneideri][more]
gi|694406561|ref|XP_009378082.1|2.9e-16066.81PREDICTED: uncharacterized protein LOC103966622 [Pyrus x bretschneideri][more]
gi|657974404|ref|XP_008379030.1|1.4e-15967.25PREDICTED: uncharacterized protein LOC103442068 [Malus domestica][more]
The following terms have been associated with this gene:
Vocabulary: Cellular Component
TermDefinition
GO:0016021integral component of membrane
Vocabulary: Biological Process
TermDefinition
GO:0015693magnesium ion transport
Vocabulary: Molecular Function
TermDefinition
GO:0015095magnesium ion transmembrane transporter activity
Vocabulary: INTERPRO
TermDefinition
IPR008521Mg_trans_NIPA
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015693 magnesium ion transport
biological_process GO:1903830 magnesium ion transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005769 early endosome
cellular_component GO:0005886 plasma membrane
molecular_function GO:0015095 magnesium ion transmembrane transporter activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G004280.1Lsi05G004280.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008521Magnesium transporter NIPAPANTHERPTHR12570UNCHARACTERIZEDcoord: 1..301
score: 7.8E
IPR008521Magnesium transporter NIPAPFAMPF05653Mg_trans_NIPAcoord: 5..164
score: 2.
NoneNo IPR availablePANTHERPTHR12570:SF9SUBFAMILY NOT NAMEDcoord: 1..301
score: 7.8E