Lsi05G003800 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi05G003800
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Cytosolic Fe-S cluster assembly factor
Location	chr05 : 4796047 .. 4798368 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGAACAGCGAAATCCCTGAAAACGCTAATGAAAGTAATGGATTTCTTTCCTGTTCATTGTTTTTGTCATTTGTAGATTGTTCAACTTATTAGCCCTTTTTTGTTTTCTGTATTTCATTTTCATTGATGTTCTCTTTGTGGTGGCTTTTTCGATTTCAGATTGCCCAGGTCCTCAGTCGGAGTCGGCAGGGAAATCCGATGCCTGTCAAGGATGTCCCAACCAATCAATTTGTGCTACTGCGCCCAAAGGCCCTGACCCAGGTTCCCTTTCTCCTCCTCTGAATCTATTTGCTTTTTGTTCACATCTAAATTAGTTTAAAGAGATTGCTTTGTTGTTACATCGCATGAGCTTTTGGGACTGATTGGGGTTTATTCTGTTTAGATGGTTTTTGAAGCTAAAGTTAATGTCAATTGTCAGTTGATGAGATTACTATCTATATGGCATTATTTGTATTTTTTTTTTTCCTGATAATCTGGGTTTTGCTTCTTCATGGCTCACCCACTACTCTTTTGGGAGACTTTTCCTGTTCCAAAATAAGTAGTTAATCAAGTTGGATTTAACTTTGGTTATATACTTTCCGCTTTTGTCCAGTTTGATCTTTGGACTACGAAGATTTCCCCTTGGCTTATGTTTTATATAATTTTTGTGATCATAATTTTAGCATAAAACACTGCACTCATAAATTTGTAAAAATAGTCAGTTCATAAATTTGTAAAAATAGTCAGTTCGTAAATGGAAGGTTTTCACCAAGTATAGAATTTGTGTTAAGATTTTACAAACATGAATTTTACAGGACACTTTTTTAGAACTCAACGGAACAGTTGTACAACTTATAGAAATGATCCTGTTCGAGTAAGGTCATTTTCTAAAAGTACAGAAACCAAAATGAGTGGATGAGTGGATGTTTTGAAAGTACCATCAAAATAGACAGGTTAGAAATATAGGGAACAAAAAGAAGTTATTTAAGTTTTCCTAAAACATTGGAGGGTTGCACAAGACACCTTTTATTTGTATTTTTTATTCTCTCAAACCAAGCAAAGATGTAAGTTAAACTTTACAAGCTTTAGAAGACGTCTCTTATTAGTTGTTACTTGTTAGTTTACTGTACTACCAGTAAGTGAAATTGTGATGTGATGGGCAGAAGTACTTTTTGCTGGCTTCTAATATTGCCTTGTTTAGAATATACCTTAGTTCTCTATCTTCCTTTTATTTGGTATAGGGTTACTCTACAATTCTACATTTTGGCCAATTGCTTCTTGGTCGTAAAGGCATTAGGGTTTGGAACTGTTTGGTTTTTTGAATCCTATGTATGAATACACTAGTCGAATCAATTAACTGTTAGTGTGTTTATGCTCTATTTACCATTCTTTCCTCACCTTTTTCCAGACTTGGTTTCAATAGCAGAACGAATGGCAACGGTTAAGCACAAGATTCTTATTCTATCAGGTAAGGGTGGGGTCGGAAAGAGCACGTTCTCTACTCAGCTTGCATTTGCGCTAGCTGCCATGGATTTTCAGGTGGGTCTCCTCGATATCGATATTTGTGGTCCGAGTATCCCGAAGATGCTTGGTCTAGAAGGTCAAGATGTGCATCAGAGTAACCTTGGATGGTCCCCTGTTTACATAGAGTCCAACCTTGGAGTTATGTCAATTGGTTTCATGCTCCCTAACCCGGACGAAGCAGTCATTTGGAGAGGCCCTCGAAAAAATGGGCTCATTAAGCAGTTTCTCAAGGATGTGTATTGGGGAGAACTTGATTTTCTAGTTGTTGATGCTCCACCAGGGACTTCAGATGAGCACATATCAATCGTACAATGCCTTGAGGCTACTGGAATTGATGGTGCAATCATTGTCACCACACCACAACAAGTGTCTCTCATCGACGTGCGAAAAGAAGTGAGTTTTTGCAAAAAAGTAGGTGTAAAAGTTCTCGGAGTTGTAGAGAACATGAGCAGCTTGTGCCAACCACTATTAGATATGAAGTTTCTGAAGACGACAGAGGCAGGAGAGCAGGCAGACGTATCAGAATGGGTGGCAGAGTACATTCGGGAAAAAGCGCCAGAGCTACTAAACTTGATTACTTGTAGTGAAGTGTTCGATAGTAGCAGGGGTGGCGCTGTGAAAATGTGTAGGGAGATGGATGTGCCATTCCTGGGAAAAGTACCATTGGATCCACAGCTTTGCAAGGCAGCTGAAGAAGGAAGGTCCTGTTTTGGTGACAAAAAATGTGGGGTAAGTGCCTCAGCACTGAAGAAGATTATAGAGAAGTTGATACAAAATCAAGGGTTTTCAACAATGTCAATAGATGCTAAAGCGTAG

mRNA sequence

ATGGAGAACAGCGAAATCCCTGAAAACGCTAATGAAAATTGCCCAGGTCCTCAGTCGGAGTCGGCAGGGAAATCCGATGCCTGTCAAGGATGTCCCAACCAATCAATTTGTGCTACTGCGCCCAAAGGCCCTGACCCAGACTTGGTTTCAATAGCAGAACGAATGGCAACGGTTAAGCACAAGATTCTTATTCTATCAGGTAAGGGTGGGGTCGGAAAGAGCACGTTCTCTACTCAGCTTGCATTTGCGCTAGCTGCCATGGATTTTCAGGTGGGTCTCCTCGATATCGATATTTGTGGTCCGAGTATCCCGAAGATGCTTGGTCTAGAAGGTCAAGATGTGCATCAGAGTAACCTTGGATGGTCCCCTGTTTACATAGAGTCCAACCTTGGAGTTATGTCAATTGGTTTCATGCTCCCTAACCCGGACGAAGCAGTCATTTGGAGAGGCCCTCGAAAAAATGGGCTCATTAAGCAGTTTCTCAAGGATGTGTATTGGGGAGAACTTGATTTTCTAGTTGTTGATGCTCCACCAGGGACTTCAGATGAGCACATATCAATCGTACAATGCCTTGAGGCTACTGGAATTGATGGTGCAATCATTGTCACCACACCACAACAAGTGTCTCTCATCGACGTGCGAAAAGAAGTGAGTTTTTGCAAAAAAGTAGGTGTAAAAGTTCTCGGAGTTGTAGAGAACATGAGCAGCTTGTGCCAACCACTATTAGATATGAAGTTTCTGAAGACGACAGAGGCAGGAGAGCAGGCAGACGTATCAGAATGGGTGGCAGAGTACATTCGGGAAAAAGCGCCAGAGCTACTAAACTTGATTACTTGTAGTGAAGTGTTCGATAGTAGCAGGGGTGGCGCTGTGAAAATGTGTAGGGAGATGGATGTGCCATTCCTGGGAAAAGTACCATTGGATCCACAGCTTTGCAAGGCAGCTGAAGAAGGAAGGTCCTGTTTTGGTGACAAAAAATGTGGGGTAAGTGCCTCAGCACTGAAGAAGATTATAGAGAAGTTGATACAAAATCAAGGGTTTTCAACAATGTCAATAGATGCTAAAGCGTAG

Coding sequence (CDS)

ATGGAGAACAGCGAAATCCCTGAAAACGCTAATGAAAATTGCCCAGGTCCTCAGTCGGAGTCGGCAGGGAAATCCGATGCCTGTCAAGGATGTCCCAACCAATCAATTTGTGCTACTGCGCCCAAAGGCCCTGACCCAGACTTGGTTTCAATAGCAGAACGAATGGCAACGGTTAAGCACAAGATTCTTATTCTATCAGGTAAGGGTGGGGTCGGAAAGAGCACGTTCTCTACTCAGCTTGCATTTGCGCTAGCTGCCATGGATTTTCAGGTGGGTCTCCTCGATATCGATATTTGTGGTCCGAGTATCCCGAAGATGCTTGGTCTAGAAGGTCAAGATGTGCATCAGAGTAACCTTGGATGGTCCCCTGTTTACATAGAGTCCAACCTTGGAGTTATGTCAATTGGTTTCATGCTCCCTAACCCGGACGAAGCAGTCATTTGGAGAGGCCCTCGAAAAAATGGGCTCATTAAGCAGTTTCTCAAGGATGTGTATTGGGGAGAACTTGATTTTCTAGTTGTTGATGCTCCACCAGGGACTTCAGATGAGCACATATCAATCGTACAATGCCTTGAGGCTACTGGAATTGATGGTGCAATCATTGTCACCACACCACAACAAGTGTCTCTCATCGACGTGCGAAAAGAAGTGAGTTTTTGCAAAAAAGTAGGTGTAAAAGTTCTCGGAGTTGTAGAGAACATGAGCAGCTTGTGCCAACCACTATTAGATATGAAGTTTCTGAAGACGACAGAGGCAGGAGAGCAGGCAGACGTATCAGAATGGGTGGCAGAGTACATTCGGGAAAAAGCGCCAGAGCTACTAAACTTGATTACTTGTAGTGAAGTGTTCGATAGTAGCAGGGGTGGCGCTGTGAAAATGTGTAGGGAGATGGATGTGCCATTCCTGGGAAAAGTACCATTGGATCCACAGCTTTGCAAGGCAGCTGAAGAAGGAAGGTCCTGTTTTGGTGACAAAAAATGTGGGGTAAGTGCCTCAGCACTGAAGAAGATTATAGAGAAGTTGATACAAAATCAAGGGTTTTCAACAATGTCAATAGATGCTAAAGCGTAG

Protein sequence

MENSEIPENANENCPGPQSESAGKSDACQGCPNQSICATAPKGPDPDLVSIAERMATVKHKILILSGKGGVGKSTFSTQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLGWSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGTSDEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSSLCQPLLDMKFLKTTEAGEQADVSEWVAEYIREKAPELLNLITCSEVFDSSRGGAVKMCREMDVPFLGKVPLDPQLCKAAEEGRSCFGDKKCGVSASALKKIIEKLIQNQGFSTMSIDAKA

Homology

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BLAST of Lsi05G003800 vs. Swiss-Prot
Match: NBP35_ARATH (Cytosolic Fe-S cluster assembly factor NBP35 OS=Arabidopsis thaliana GN=NBP35 PE=1 SV=1)

HSP 1 Score: 568.5 bits (1464), Expect = 5.0e-161
Identity = 275/343 (80.17%), Postives = 307/343 (89.50%), Query Frame = 1


Query: 1   MENSEIPENANENCPGPQSESAGKSDACQGCPNQSICATAPKGPDPDLVSIAERMATVKH 60
           MEN +IPE+ANE+CPGPQSESAGKSD+C GCPNQ  CATAPKGPDPDLV+IAERM+TVKH
Sbjct: 1   MENGDIPEDANEHCPGPQSESAGKSDSCAGCPNQEACATAPKGPDPDLVAIAERMSTVKH 60

Query: 61  KILILSGKGGVGKSTFSTQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLG 120
           KIL+LSGKGGVGKSTFS QL+FALA MD QVGL+DIDICGPSIPKMLGLEGQ++HQSNLG
Sbjct: 61  KILVLSGKGGVGKSTFSAQLSFALAGMDHQVGLMDIDICGPSIPKMLGLEGQEIHQSNLG 120

Query: 121 WSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGT 180
           WSPVY+E NLGVMSIGFMLPN DEAVIWRGPRKNGLIKQFLKDVYWGE+D+LVVDAPPGT
Sbjct: 121 WSPVYVEDNLGVMSIGFMLPNSDEAVIWRGPRKNGLIKQFLKDVYWGEIDYLVVDAPPGT 180

Query: 181 SDEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSSLCQP 240
           SDEHISIVQ L  TGIDGAIIVTTPQ+VSLIDVRKEVSFCKKVGV VLGVVENMS L QP
Sbjct: 181 SDEHISIVQYLLPTGIDGAIIVTTPQEVSLIDVRKEVSFCKKVGVPVLGVVENMSGLSQP 240

Query: 241 LLDMKFLK-TTEAGEQADVSEWVAEYIREKAPELLNLITCSEVFDSSRGGAVKMCREMDV 300
           L D+KF+K  TE G   +V+E V   +R+ APELL+++ CSEVFDSS GGA +MCREM V
Sbjct: 241 LKDVKFMKLATETGSSINVTEDVIACLRKNAPELLDIVACSEVFDSSGGGAERMCREMGV 300

Query: 301 PFLGKVPLDPQLCKAAEEGRSCFGDKKCGVSASALKKIIEKLI 343
           PFLGKVP+DPQLCKAAE+G+SCF D KC +SA ALK II+K++
Sbjct: 301 PFLGKVPMDPQLCKAAEQGKSCFEDNKCLISAPALKSIIQKVV 343

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BLAST of Lsi05G003800 vs. Swiss-Prot
Match: NUBP1_DICDI (Cytosolic Fe-S cluster assembly factor NUBP1 homolog OS=Dictyostelium discoideum GN=nubp1 PE=3 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 5.4e-99
Identity = 175/310 (56.45%), Postives = 231/310 (74.52%), Query Frame = 1


Query: 12  ENCPGPQSESAGKSDACQGCPNQSICATAPKGPDPDLVSIAERMATVKHKILILSGKGGV 71
           ENCPG QSE +GKS AC GCPNQ ICATAPKGPDPD++ I ERM TVK+KIL+LSGKGGV
Sbjct: 11  ENCPGTQSEMSGKSAACAGCPNQQICATAPKGPDPDIIEIEERMKTVKNKILVLSGKGGV 70

Query: 72  GKSTFSTQLAFALAAMD-FQVGLLDIDICGPSIPKMLGLEGQDVHQSNLGWSPVYIESNL 131
           GKSTFS+QL+FAL+  +  +VGLLDIDICGPSIPK++GLEG+++H S  GW PVY++ NL
Sbjct: 71  GKSTFSSQLSFALSMDEKVEVGLLDIDICGPSIPKIMGLEGENIHISGQGWDPVYVQDNL 130

Query: 132 GVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGTSDEHISIVQC 191
            VMS+GF+L   ++AVIWRGP+KNG+IKQFLKDVYW +LD+LV+D PPGTSDEH+SIVQ 
Sbjct: 131 AVMSVGFLLEKEEDAVIWRGPKKNGIIKQFLKDVYWNDLDYLVIDTPPGTSDEHLSIVQY 190

Query: 192 LEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSSLCQPLLDMKFLKTT 251
           L+ + + GA+IVT+PQ V+LIDVRKE++FCKKVGV              P++        
Sbjct: 191 LKTSNLSGAVIVTSPQDVALIDVRKEINFCKKVGV--------------PII-------- 250

Query: 252 EAGEQADVSEWVAEYIREKAPELLNLITCSEVFDSSRGGAVKMCREMDVPFLGKVPLDPQ 311
             G   ++S +V     ++          S++F  + GGA KM ++M+VPFLG++P+DP 
Sbjct: 251 --GVVENMSGFVCPKCNKE----------SQIFIPTTGGAEKMSQDMNVPFLGRIPIDPL 286

Query: 312 LCKAAEEGRS 321
           + ++ +EG+S
Sbjct: 311 IARSCDEGKS 286

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BLAST of Lsi05G003800 vs. Swiss-Prot
Match: NUP1A_XENLA (Cytosolic Fe-S cluster assembly factor nubp1-A OS=Xenopus laevis GN=nubp1-A PE=2 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 1.3e-97
Identity = 178/340 (52.35%), Postives = 237/340 (69.71%), Query Frame = 1


Query: 4   SEIPENANENCPGPQSESAGKSDACQGCPNQSICAT-APKGPDPDLVSIAERMATVKHKI 63
           ++IP+NA ++CPG  S  AGKS ACQGCPNQSICA+ A  GPDP +  I E+M+ VKHKI
Sbjct: 2   ADIPDNAPQHCPGTDSTEAGKSSACQGCPNQSICASGAAAGPDPAIEEIKEKMSLVKHKI 61

Query: 64  LILSGKGGVGKSTFSTQLAFALAAMDF-QVGLLDIDICGPSIPKMLGLEGQDVHQSNLGW 123
           L+LSGKGGVGKSTFS  LA  LA  +  +V LLD+DICGPSIPKM+GLEG+ VHQS  GW
Sbjct: 62  LVLSGKGGVGKSTFSAHLAHGLAQDEGKEVALLDVDICGPSIPKMMGLEGEQVHQSGSGW 121

Query: 124 SPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGTS 183
           SPVY+E NL VMS+GF+L +PD+AVIWRGP+KNG+IKQFL+DV WG++D+L+VD PPGTS
Sbjct: 122 SPVYVEDNLAVMSVGFLLSSPDDAVIWRGPKKNGMIKQFLRDVDWGDVDYLIVDTPPGTS 181

Query: 184 DEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSSLCQPL 243
           DEH+S+VQ L A GIDGA+I+TTPQ+VSL DVRKE++FC+KV + +              
Sbjct: 182 DEHLSVVQYLSAAGIDGAVIITTPQEVSLQDVRKEINFCRKVKLPI-------------- 241

Query: 244 LDMKFLKTTEAGEQADVSEWVAEYIREKAPELLNLITCSEVFDSSRGGAVKMCREMDVPF 303
                      G   ++S ++    + +          S++F  + GGA KMC ++ V  
Sbjct: 242 ----------IGVVENMSGFICPKCKNE----------SQIFPPTTGGAEKMCTDLSVSL 301

Query: 304 LGKVPLDPQLCKAAEEGRSCFGDKKCGVSASALKKIIEKL 342
           LGKVPLDP + K+ + G+S F +     +  + +KII+++
Sbjct: 302 LGKVPLDPNIGKSCDTGKSFFTEIPDSPATLSYRKIIQRI 307

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BLAST of Lsi05G003800 vs. Swiss-Prot
Match: NUBP1_NEMVE (Cytosolic Fe-S cluster assembly factor NUBP1 homolog OS=Nematostella vectensis GN=v1g236650 PE=3 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 1.1e-96
Identity = 179/342 (52.34%), Postives = 238/342 (69.59%), Query Frame = 1


Query: 4   SEIPENANENCPGPQSESAGKSDACQGCPNQSICATA-PKGPDPDLVSIAERMATVKHKI 63
           +++P++A E+CPG +S+ +GK  ACQGCPNQ ICA++ P  PDPDL  I ER+++VKHKI
Sbjct: 2   ADVPKDAPEHCPGTESDKSGKVSACQGCPNQQICASSKPAPPDPDLGKIKERLSSVKHKI 61

Query: 64  LILSGKGGVGKSTFSTQLAFALAA-MDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLGW 123
           L+LSGKGGVGKSTF+  LA  LAA  D QV +LDIDICGPSIP + GL+G+ VHQS  GW
Sbjct: 62  LVLSGKGGVGKSTFTAHLAHGLAADEDRQVAVLDIDICGPSIPTVFGLQGEQVHQSGSGW 121

Query: 124 SPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGTS 183
           SPVY+E NLGVMS+GF+L  P +AVIWRGP+KNGLIKQFL+DV WG+ DFLVVD PPGTS
Sbjct: 122 SPVYVEDNLGVMSVGFLLAKPTDAVIWRGPKKNGLIKQFLRDVDWGDADFLVVDTPPGTS 181

Query: 184 DEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSSLCQPL 243
           DEH+SI+Q L  T +DGA++VTTPQ+VSL+DVRKE+SF                      
Sbjct: 182 DEHLSIIQYLNQTEVDGAVVVTTPQEVSLLDVRKEISF---------------------- 241

Query: 244 LDMKFLKTTEAGEQADVSEWVAEYIREKAPELLNLITCSEVFDSSRGGAVKMCREMDVPF 303
              K ++    G   ++S +V    +++          S++F  + GGA KM  EM VPF
Sbjct: 242 --CKKVRLPVIGVVENMSVFVCPNCKKE----------SQIFPPTTGGAEKMAVEMKVPF 301

Query: 304 LGKVPLDPQLCKAAEEGRSCFGDKKCGVSASALKKIIEKLIQ 344
           LG++PLDP++ +A +EG+S   +     + S+ K IIEK+++
Sbjct: 302 LGRIPLDPRIGRACDEGKSFLSEIPDSPATSSYKDIIEKIVK 309

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BLAST of Lsi05G003800 vs. Swiss-Prot
Match: NUBP1_DANRE (Cytosolic Fe-S cluster assembly factor nubp1 OS=Danio rerio GN=nubp1 PE=2 SV=2)

HSP 1 Score: 351.7 bits (901), Expect = 9.6e-96
Identity = 178/340 (52.35%), Postives = 239/340 (70.29%), Query Frame = 1


Query: 4   SEIPENANENCPGPQSESAGKSDACQGCPNQSICAT-APKGPDPDLVSIAERMATVKHKI 63
           +++P +A E+CPG  S+ AGKS ACQGCPNQSICA+ A K PDP +  I ++M +VKHKI
Sbjct: 2   ADVPNDAPEHCPGTSSDQAGKSSACQGCPNQSICASGATKAPDPAIEEIKQKMTSVKHKI 61

Query: 64  LILSGKGGVGKSTFSTQLAFALAA-MDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLGW 123
           L+LSGKGGVGKSTFS  L+ ALA+    +V LLD+DICGPSIPK++GLEG+ VHQS  GW
Sbjct: 62  LVLSGKGGVGKSTFSAHLSHALASDSSKEVALLDVDICGPSIPKIMGLEGEQVHQSGSGW 121

Query: 124 SPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGTS 183
           SPVY+E NL VMSIGF+L +PD+AVIWRGP+KNG+IKQFL+DV WGE+D+L+VD PPGTS
Sbjct: 122 SPVYVEDNLAVMSIGFLLSSPDDAVIWRGPKKNGMIKQFLRDVDWGEVDYLIVDTPPGTS 181

Query: 184 DEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSSLCQPL 243
           DEH+SIVQ L   GIDGA+I+TTPQ+VSL DVRKE+ F                 +  P+
Sbjct: 182 DEHLSIVQYLSGAGIDGAVIITTPQEVSLQDVRKEIRF--------------CKKVNLPI 241

Query: 244 LDMKFLKTTEAGEQADVSEWVAEYIREKAPELLNLITCSEVFDSSRGGAVKMCREMDVPF 303
           L          G   ++S +V        P+  N    S++F  + GGA +MC E+++P 
Sbjct: 242 L----------GVIENMSGFVC-------PKCKNT---SQIFPPTTGGAQRMCEELNLPL 301

Query: 304 LGKVPLDPQLCKAAEEGRSCFGDKKCGVSASALKKIIEKL 342
           LG++PLDP++ K+ +EG+S   +     +A+A + I++K+
Sbjct: 302 LGRIPLDPRIGKSCDEGKSFLTEVPDSPAAAAYQSIVQKI 307

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BLAST of Lsi05G003800 vs. TrEMBL
Match: M5WSX8_PRUPE (Cytosolic Fe-S cluster assembly factor NBP35 OS=Prunus persica GN=NBP35 PE=3 SV=1)

HSP 1 Score: 633.3 bits (1632), Expect = 1.8e-178
Identity = 300/356 (84.27%), Postives = 332/356 (93.26%), Query Frame = 1


Query: 1   MENSEIPENANENCPGPQSESAGKSDACQGCPNQSICATAPKGPDPDLVSIAERMATVKH 60
           MEN EIPENANE+CPGPQS+SAGKSDAC+GCPNQ ICATAPKGPDPD+V+IAERMATVKH
Sbjct: 1   MENGEIPENANEHCPGPQSDSAGKSDACEGCPNQQICATAPKGPDPDVVAIAERMATVKH 60

Query: 61  KILILSGKGGVGKSTFSTQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLG 120
           KIL+LSGKGGVGKSTFS QLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQ++HQSNLG
Sbjct: 61  KILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQEIHQSNLG 120

Query: 121 WSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGT 180
           WSPVY+ESNLGVMSIGFMLP PD+AVIWRGPRKN LIKQFL+DVYWGELDFLVVDAPPGT
Sbjct: 121 WSPVYVESNLGVMSIGFMLPEPDDAVIWRGPRKNALIKQFLRDVYWGELDFLVVDAPPGT 180

Query: 181 SDEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSSLCQP 240
           SDEHISIVQCL+A  IDGAIIVTTPQQVSLIDVRKEVSFCKKVGV+VLG+VENMS LCQP
Sbjct: 181 SDEHISIVQCLDAGSIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVQVLGIVENMSGLCQP 240

Query: 241 LLDMKFLKTTEAGEQADVSEWVAEYIREKAPELLNLITCSEVFDSSRGGAVKMCREMDVP 300
           ++D KFL+ TEAGEQ DV+EW  E+++E+APE L++I C+EVFDSSRGGA KMCREM VP
Sbjct: 241 VMDFKFLRMTEAGEQIDVTEWAREHLKERAPEFLSIIACTEVFDSSRGGAEKMCREMGVP 300

Query: 301 FLGKVPLDPQLCKAAEEGRSCFGDKKCGVSASALKKIIEKLIQNQGFSTMSIDAKA 357
           FLGKVPLDPQLCKAAEEGRSCF D+KCGVSA ALKK+I+K+++NQG S M +D  A
Sbjct: 301 FLGKVPLDPQLCKAAEEGRSCFVDQKCGVSAPALKKVIDKMMENQGLSRMLVDNNA 356

[Close]

BLAST of Lsi05G003800 vs. TrEMBL
Match: A0A140B4A8_PRUPE (Cytosolic Fe-S cluster assembly factor NBP35 OS=Prunus persica var. persica GN=NBP35-2 PE=2 SV=1)

HSP 1 Score: 633.3 bits (1632), Expect = 1.8e-178
Identity = 300/356 (84.27%), Postives = 332/356 (93.26%), Query Frame = 1


Query: 1   MENSEIPENANENCPGPQSESAGKSDACQGCPNQSICATAPKGPDPDLVSIAERMATVKH 60
           MEN EIPENANE+CPGPQS+SAGKSDAC+GCPNQ ICATAPKGPDPD+V+IAERMATVKH
Sbjct: 1   MENGEIPENANEHCPGPQSDSAGKSDACEGCPNQQICATAPKGPDPDVVAIAERMATVKH 60

Query: 61  KILILSGKGGVGKSTFSTQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLG 120
           KIL+LSGKGGVGKSTFS QLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQ++HQSNLG
Sbjct: 61  KILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQEIHQSNLG 120

Query: 121 WSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGT 180
           WSPVY+ESNLGVMSIGFMLP PD+AVIWRGPRKN LIKQFL+DVYWGELDFLVVDAPPGT
Sbjct: 121 WSPVYVESNLGVMSIGFMLPEPDDAVIWRGPRKNALIKQFLRDVYWGELDFLVVDAPPGT 180

Query: 181 SDEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSSLCQP 240
           SDEHISIVQCL+A  IDGAIIVTTPQQVSLIDVRKEVSFCKKVGV+VLG+VENMS LCQP
Sbjct: 181 SDEHISIVQCLDAGSIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVQVLGIVENMSGLCQP 240

Query: 241 LLDMKFLKTTEAGEQADVSEWVAEYIREKAPELLNLITCSEVFDSSRGGAVKMCREMDVP 300
           ++D KFL+ TEAGEQ DV+EW  E+++E+APE L++I C+EVFDSSRGGA KMCREM VP
Sbjct: 241 VMDFKFLRMTEAGEQIDVTEWAREHLKERAPEFLSIIACTEVFDSSRGGAEKMCREMGVP 300

Query: 301 FLGKVPLDPQLCKAAEEGRSCFGDKKCGVSASALKKIIEKLIQNQGFSTMSIDAKA 357
           FLGKVPLDPQLCKAAEEGRSCF D+KCGVSA ALKK+I+K+++NQG S M +D  A
Sbjct: 301 FLGKVPLDPQLCKAAEEGRSCFVDQKCGVSAPALKKVIDKMMENQGLSRMLVDNNA 356

[Close]

BLAST of Lsi05G003800 vs. TrEMBL
Match: F6I5M0_VITVI (Cytosolic Fe-S cluster assembly factor NBP35 OS=Vitis vinifera GN=NBP35 PE=3 SV=1)

HSP 1 Score: 626.7 bits (1615), Expect = 1.7e-176
Identity = 299/348 (85.92%), Postives = 325/348 (93.39%), Query Frame = 1


Query: 2   ENSEIPENANENCPGPQSESAGKSDACQGCPNQSICATAPKGPDPDLVSIAERMATVKHK 61
           ++++IPENANE+CPGPQSESAG+SDACQGCPNQ  CATAPKGPDPDLV+IAERMATVK K
Sbjct: 5   DSNQIPENANEHCPGPQSESAGQSDACQGCPNQEACATAPKGPDPDLVAIAERMATVKRK 64

Query: 62  ILILSGKGGVGKSTFSTQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLGW 121
           IL+LSGKGGVGKSTFS QL+FALAAMDFQVGLLDIDICGPSIPKMLGLEGQD+HQSNLGW
Sbjct: 65  ILVLSGKGGVGKSTFSAQLSFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDIHQSNLGW 124

Query: 122 SPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGTS 181
           SPVY+ESNLGVMSIGFMLPNPDEAVIWRGPRKNG+IKQFLKDVYWGELDFLVVDAPPGTS
Sbjct: 125 SPVYVESNLGVMSIGFMLPNPDEAVIWRGPRKNGIIKQFLKDVYWGELDFLVVDAPPGTS 184

Query: 182 DEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSSLCQPL 241
           DEHISIVQ L+ TGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGV+VLGVVENMS LCQP+
Sbjct: 185 DEHISIVQFLQGTGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVEVLGVVENMSGLCQPV 244

Query: 242 LDMKFLKTTEAGEQADVSEWVAEYIREKAPELLNLITCSEVFDSSRGGAVKMCREMDVPF 301
            D +F + TE GEQ DV+EWV EY+REKAPE+LNL+ CSEVFDSS GGA KMC+EM+VPF
Sbjct: 245 TDFRFTRVTETGEQKDVTEWVIEYMREKAPEMLNLVACSEVFDSSSGGAAKMCKEMNVPF 304

Query: 302 LGKVPLDPQLCKAAEEGRSCFGDKKCGVSASALKKIIEKLIQNQGFST 350
           LGKVPLDPQLCKAAEEGRSCF D KCGVSA AL++IIEKL+ NQ  ST
Sbjct: 305 LGKVPLDPQLCKAAEEGRSCFADTKCGVSAPALQRIIEKLVANQALST 352

[Close]

BLAST of Lsi05G003800 vs. TrEMBL
Match: A0A0S3RQ06_PHAAN (Cytosolic Fe-S cluster assembly factor NBP35 OS=Vigna angularis var. angularis GN=Vigan.03G254000 PE=3 SV=1)

HSP 1 Score: 626.7 bits (1615), Expect = 1.7e-176
Identity = 298/347 (85.88%), Postives = 326/347 (93.95%), Query Frame = 1


Query: 1   MENSEIPENANENCPGPQSESAGKSDACQGCPNQSICATAPKGPDPDLVSIAERMATVKH 60
           MEN +IPENANE+CPGPQSESAGKSDAC+GCPNQ ICATAPKGPDPDLV+IAERMATVKH
Sbjct: 1   MENGDIPENANEHCPGPQSESAGKSDACEGCPNQQICATAPKGPDPDLVAIAERMATVKH 60

Query: 61  KILILSGKGGVGKSTFSTQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLG 120
           KIL+LSGKGGVGKSTFS QLAFALAA DFQVGLLD+DICGPSIPKMLGLEGQ++HQSNLG
Sbjct: 61  KILVLSGKGGVGKSTFSAQLAFALAARDFQVGLLDVDICGPSIPKMLGLEGQEIHQSNLG 120

Query: 121 WSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGT 180
           WSPVY+ESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFL+VDAPPGT
Sbjct: 121 WSPVYVESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLIVDAPPGT 180

Query: 181 SDEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSSLCQP 240
           SDEHISIVQCL+ATG+DGAIIVTTPQQVSLIDVRKEV+FCKKVGVKVLGVVENMSSLCQP
Sbjct: 181 SDEHISIVQCLDATGVDGAIIVTTPQQVSLIDVRKEVNFCKKVGVKVLGVVENMSSLCQP 240

Query: 241 LLDMKFLKTTEAGEQADVSEWVAEYIREKAPELLNLITCSEVFDSSRGGAVKMCREMDVP 300
           + D KF++ ++ GEQ DV++WV EY++EKAPE+LNLI C+EVFDS  GGAVKMC EM VP
Sbjct: 241 ITDFKFMRMSDNGEQKDVTQWVWEYMKEKAPEMLNLIACTEVFDSRGGGAVKMCNEMGVP 300

Query: 301 FLGKVPLDPQLCKAAEEGRSCFGDKKCGVSASALKKIIEKLIQNQGF 348
           FLGKVPLDPQLCKAAEEG SCF +K C VSA ALKKIIE+LI+N G+
Sbjct: 301 FLGKVPLDPQLCKAAEEGTSCFANKDCVVSAPALKKIIEELIENNGW 347

[Close]

BLAST of Lsi05G003800 vs. TrEMBL
Match: A0A0L9V1F3_PHAAN (Cytosolic Fe-S cluster assembly factor NBP35 OS=Phaseolus angularis GN=NBP35 PE=3 SV=1)

HSP 1 Score: 626.7 bits (1615), Expect = 1.7e-176
Identity = 298/347 (85.88%), Postives = 326/347 (93.95%), Query Frame = 1


Query: 1   MENSEIPENANENCPGPQSESAGKSDACQGCPNQSICATAPKGPDPDLVSIAERMATVKH 60
           MEN +IPENANE+CPGPQSESAGKSDAC+GCPNQ ICATAPKGPDPDLV+IAERMATVKH
Sbjct: 1   MENGDIPENANEHCPGPQSESAGKSDACEGCPNQQICATAPKGPDPDLVAIAERMATVKH 60

Query: 61  KILILSGKGGVGKSTFSTQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLG 120
           KIL+LSGKGGVGKSTFS QLAFALAA DFQVGLLD+DICGPSIPKMLGLEGQ++HQSNLG
Sbjct: 61  KILVLSGKGGVGKSTFSAQLAFALAARDFQVGLLDVDICGPSIPKMLGLEGQEIHQSNLG 120

Query: 121 WSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGT 180
           WSPVY+ESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFL+VDAPPGT
Sbjct: 121 WSPVYVESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLIVDAPPGT 180

Query: 181 SDEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSSLCQP 240
           SDEHISIVQCL+ATG+DGAIIVTTPQQVSLIDVRKEV+FCKKVGVKVLGVVENMSSLCQP
Sbjct: 181 SDEHISIVQCLDATGVDGAIIVTTPQQVSLIDVRKEVNFCKKVGVKVLGVVENMSSLCQP 240

Query: 241 LLDMKFLKTTEAGEQADVSEWVAEYIREKAPELLNLITCSEVFDSSRGGAVKMCREMDVP 300
           + D KF++ ++ GEQ DV++WV EY++EKAPE+LNLI C+EVFDS  GGAVKMC EM VP
Sbjct: 241 ITDFKFMRMSDNGEQKDVTQWVWEYMKEKAPEMLNLIACTEVFDSRGGGAVKMCNEMGVP 300

Query: 301 FLGKVPLDPQLCKAAEEGRSCFGDKKCGVSASALKKIIEKLIQNQGF 348
           FLGKVPLDPQLCKAAEEG SCF +K C VSA ALKKIIE+LI+N G+
Sbjct: 301 FLGKVPLDPQLCKAAEEGTSCFANKDCVVSAPALKKIIEELIENNGW 347

[Close]

BLAST of Lsi05G003800 vs. TAIR10
Match: AT5G50960.1 (AT5G50960.1 nucleotide binding protein 35)

HSP 1 Score: 568.5 bits (1464), Expect = 2.8e-162
Identity = 275/343 (80.17%), Postives = 307/343 (89.50%), Query Frame = 1


Query: 1   MENSEIPENANENCPGPQSESAGKSDACQGCPNQSICATAPKGPDPDLVSIAERMATVKH 60
           MEN +IPE+ANE+CPGPQSESAGKSD+C GCPNQ  CATAPKGPDPDLV+IAERM+TVKH
Sbjct: 1   MENGDIPEDANEHCPGPQSESAGKSDSCAGCPNQEACATAPKGPDPDLVAIAERMSTVKH 60

Query: 61  KILILSGKGGVGKSTFSTQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLG 120
           KIL+LSGKGGVGKSTFS QL+FALA MD QVGL+DIDICGPSIPKMLGLEGQ++HQSNLG
Sbjct: 61  KILVLSGKGGVGKSTFSAQLSFALAGMDHQVGLMDIDICGPSIPKMLGLEGQEIHQSNLG 120

Query: 121 WSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGT 180
           WSPVY+E NLGVMSIGFMLPN DEAVIWRGPRKNGLIKQFLKDVYWGE+D+LVVDAPPGT
Sbjct: 121 WSPVYVEDNLGVMSIGFMLPNSDEAVIWRGPRKNGLIKQFLKDVYWGEIDYLVVDAPPGT 180

Query: 181 SDEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSSLCQP 240
           SDEHISIVQ L  TGIDGAIIVTTPQ+VSLIDVRKEVSFCKKVGV VLGVVENMS L QP
Sbjct: 181 SDEHISIVQYLLPTGIDGAIIVTTPQEVSLIDVRKEVSFCKKVGVPVLGVVENMSGLSQP 240

Query: 241 LLDMKFLK-TTEAGEQADVSEWVAEYIREKAPELLNLITCSEVFDSSRGGAVKMCREMDV 300
           L D+KF+K  TE G   +V+E V   +R+ APELL+++ CSEVFDSS GGA +MCREM V
Sbjct: 241 LKDVKFMKLATETGSSINVTEDVIACLRKNAPELLDIVACSEVFDSSGGGAERMCREMGV 300

Query: 301 PFLGKVPLDPQLCKAAEEGRSCFGDKKCGVSASALKKIIEKLI 343
           PFLGKVP+DPQLCKAAE+G+SCF D KC +SA ALK II+K++
Sbjct: 301 PFLGKVPMDPQLCKAAEQGKSCFEDNKCLISAPALKSIIQKVV 343

[Close]

BLAST of Lsi05G003800 vs. TAIR10
Match: AT4G19540.1 (AT4G19540.1 IND1(iron-sulfur protein required for NADH dehydrogenase)-like)

HSP 1 Score: 143.7 bits (361), Expect = 2.2e-34
Identity = 84/190 (44.21%), Postives = 114/190 (60.00%), Query Frame = 1


Query: 54  RMATVKHKILILSGKGGVGKSTFSTQLAFALA-AMDFQVGLLDIDICGPSIPKMLGLEGQ 113
           R+  VK  I + SGKGGVGKS+ +  LA ALA   + ++GLLD D+ GPS+P M+ +  +
Sbjct: 38  RLHGVKDIIAVASGKGGVGKSSTAVNLAVALANKCELKIGLLDADVYGPSVPIMMNINQK 97

Query: 114 DVHQSNLGWSPVYIESNLGV--MSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELD 173
                ++   PV    N GV  MS+G +L   D  ++WRGP     + +  K V WG+LD
Sbjct: 98  PQVNQDMKMIPV---ENYGVKCMSMG-LLVEKDAPLVWRGPMVMSALAKMTKGVDWGDLD 157

Query: 174 FLVVDAPPGTSDEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGV 233
            LVVD PPGT D  ISI Q L+   + GA+IV+TPQ V+L D  + +S   KV V +LG+
Sbjct: 158 ILVVDMPPGTGDAQISISQNLK---LSGAVIVSTPQDVALADANRGISMFDKVRVPILGL 217

Query: 234 VENMSSLCQP 241
           VENMS    P
Sbjct: 218 VENMSCFVCP 220

[Close]

BLAST of Lsi05G003800 vs. TAIR10
Match: AT3G24430.1 (AT3G24430.1 ATP binding)

HSP 1 Score: 129.4 bits (324), Expect = 4.4e-30
Identity = 71/180 (39.44%), Postives = 104/180 (57.78%), Query Frame = 1


Query: 55  MATVKHKILILSGKGGVGKSTFSTQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDV 114
           ++ + + I + S KGGVGKST +  LA+ LA M  +VG+ D D+ GPS+P M+  E + +
Sbjct: 172 LSRISNIIAVSSCKGGVGKSTVAVNLAYTLAGMGARVGIFDADVYGPSLPTMVNPESR-I 231

Query: 115 HQSNLGWSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVV 174
            + N     +     +GV  + F       A++ RGP  +G+I Q L    WGELD+LV+
Sbjct: 232 LEMNPEKKTIIPTEYMGVKLVSFGFAGQGRAIM-RGPMVSGVINQLLTTTEWGELDYLVI 291

Query: 175 DAPPGTSDEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENM 234
           D PPGT D  +++ Q    T    A+IVTTPQ+++ IDV K V    K+ V  + VVENM
Sbjct: 292 DMPPGTGDIQLTLCQVAPLT---AAVIVTTPQKLAFIDVAKGVRMFSKLKVPCVAVVENM 346

[Close]

BLAST of Lsi05G003800 vs. TAIR10
Match: AT5G24020.1 (AT5G24020.1 septum site-determining protein (MIND))

HSP 1 Score: 54.7 bits (130), Expect = 1.4e-07
Identity = 46/182 (25.27%), Postives = 82/182 (45.05%), Query Frame = 1


Query: 62  ILILSGKGGVGKSTFSTQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQ------DVH 121
           ++I SGKGGVGK+T +  +  +LA   F V  +D D+   ++  +LGLE +      +V 
Sbjct: 61  VVITSGKGGVGKTTTTANVGLSLARYGFSVVAIDADLGLRNLDLLLGLENRVNYTCVEVI 120

Query: 122 QSNLGWSPVYIE----SNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDF 181
             +       +     SN  ++ I    P     + + G     L+   LK    G  DF
Sbjct: 121 NGDCRLDQALVRDKRWSNFELLCIS--KPRSKLPMGFGGKALEWLV-DALKTRPEGSPDF 180

Query: 182 LVVDAPPGTSDEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVV 234
           +++D P G     I+ +     T  + A++VTTP   +L D  +     +  G++ + ++
Sbjct: 181 IIIDCPAGIDAGFITAI-----TPANEAVLVTTPDITALRDADRVTGLLECDGIRDIKMI 234

[Close]

BLAST of Lsi05G003800 vs. NCBI nr
Match: gi|449453858|ref|XP_004144673.1| (PREDICTED: cytosolic Fe-S cluster assembly factor NBP35 [Cucumis sativus])

HSP 1 Score: 696.4 bits (1796), Expect = 2.5e-197
Identity = 343/356 (96.35%), Postives = 349/356 (98.03%), Query Frame = 1


Query: 1   MENSEIPENANENCPGPQSESAGKSDACQGCPNQSICATAPKGPDPDLVSIAERMATVKH 60
           MENSEIPENANENCPGP+SESAGKSDACQGCPNQSICATAPKGPDPDL+SIAERMATVKH
Sbjct: 1   MENSEIPENANENCPGPESESAGKSDACQGCPNQSICATAPKGPDPDLISIAERMATVKH 60

Query: 61  KILILSGKGGVGKSTFSTQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLG 120
           KIL+LSGKGGVGKSTFS QLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLG
Sbjct: 61  KILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLG 120

Query: 121 WSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGT 180
           WSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGT
Sbjct: 121 WSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGT 180

Query: 181 SDEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSSLCQP 240
           SDEHISIVQCL+ATGIDGAIIVTTPQQVSLIDVRKEVSFCK VGVKVLGVVENMSSLCQP
Sbjct: 181 SDEHISIVQCLKATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGVKVLGVVENMSSLCQP 240

Query: 241 LLDMKFLKTTEAGEQADVSEWVAEYIREKAPELLNLITCSEVFDSSRGGAVKMCREMDVP 300
           LLDMKFLKTTEAGEQ DV+EWV EYIREKAPELLNLIT SEVFDSS GGA KMCREMDVP
Sbjct: 241 LLDMKFLKTTEAGEQTDVTEWVGEYIREKAPELLNLITYSEVFDSSGGGAAKMCREMDVP 300

Query: 301 FLGKVPLDPQLCKAAEEGRSCFGDKKCGVSASALKKIIEKLIQNQGFSTMSIDAKA 357
           FLGKVPLDPQLCKAAEEGRSCFGD+KCGVSASALKKIIEKLIQNQGFSTMSIDAKA
Sbjct: 301 FLGKVPLDPQLCKAAEEGRSCFGDQKCGVSASALKKIIEKLIQNQGFSTMSIDAKA 356

[Close]

BLAST of Lsi05G003800 vs. NCBI nr
Match: gi|659083114|ref|XP_008442190.1| (PREDICTED: cytosolic Fe-S cluster assembly factor NBP35 [Cucumis melo])

HSP 1 Score: 694.9 bits (1792), Expect = 7.4e-197
Identity = 342/356 (96.07%), Postives = 349/356 (98.03%), Query Frame = 1


Query: 1   MENSEIPENANENCPGPQSESAGKSDACQGCPNQSICATAPKGPDPDLVSIAERMATVKH 60
           MENSEIPENANENCPGP+SESAGKSDACQGCPNQSICATAPKGPDPDL+SIAERMATVKH
Sbjct: 1   MENSEIPENANENCPGPESESAGKSDACQGCPNQSICATAPKGPDPDLISIAERMATVKH 60

Query: 61  KILILSGKGGVGKSTFSTQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLG 120
           KIL+LSGKGGVGKSTFS QLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLG
Sbjct: 61  KILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLG 120

Query: 121 WSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGT 180
           WSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGT
Sbjct: 121 WSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGT 180

Query: 181 SDEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSSLCQP 240
           SDEHISIVQCL+ATGIDGAIIVTTPQQVSLIDVRKEVSFCK VGV+VLGVVENMSSLCQP
Sbjct: 181 SDEHISIVQCLKATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGVEVLGVVENMSSLCQP 240

Query: 241 LLDMKFLKTTEAGEQADVSEWVAEYIREKAPELLNLITCSEVFDSSRGGAVKMCREMDVP 300
           LLDMKFLKTTEAGEQ DV+EWV EYIREKAPELLNLIT SEVFDSS GGA KMCREMDVP
Sbjct: 241 LLDMKFLKTTEAGEQTDVTEWVGEYIREKAPELLNLITYSEVFDSSGGGAAKMCREMDVP 300

Query: 301 FLGKVPLDPQLCKAAEEGRSCFGDKKCGVSASALKKIIEKLIQNQGFSTMSIDAKA 357
           FLGKVPLDPQLCKAAEEGRSCFGD+KCGVSASALKKIIEKLIQNQGFSTMSIDAKA
Sbjct: 301 FLGKVPLDPQLCKAAEEGRSCFGDQKCGVSASALKKIIEKLIQNQGFSTMSIDAKA 356

[Close]

BLAST of Lsi05G003800 vs. NCBI nr
Match: gi|1021559407|ref|XP_016170986.1| (PREDICTED: cytosolic Fe-S cluster assembly factor NBP35 [Arachis ipaensis])

HSP 1 Score: 633.6 bits (1633), Expect = 2.0e-178
Identity = 302/348 (86.78%), Postives = 327/348 (93.97%), Query Frame = 1


Query: 1   MENSEIPENANENCPGPQSESAGKSDACQGCPNQSICATAPKGPDPDLVSIAERMATVKH 60
           M+N +IPENANE+CPGPQSESAGKSDAC+GCPNQ ICATAPKGPDPDLV+IAERMATVKH
Sbjct: 1   MDNGDIPENANEHCPGPQSESAGKSDACEGCPNQQICATAPKGPDPDLVAIAERMATVKH 60

Query: 61  KILILSGKGGVGKSTFSTQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLG 120
           KIL+LSGKGGVGKSTFS QLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQ++HQSNLG
Sbjct: 61  KILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQEIHQSNLG 120

Query: 121 WSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGT 180
           WSPVY+ESNLGVMSIGFMLPNPD+AVIWRGPRKNGLIKQFLKDVYWGELDFL+VDAPPGT
Sbjct: 121 WSPVYVESNLGVMSIGFMLPNPDDAVIWRGPRKNGLIKQFLKDVYWGELDFLIVDAPPGT 180

Query: 181 SDEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSSLCQP 240
           SDEHISIVQCL+ATG+DGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMS LCQP
Sbjct: 181 SDEHISIVQCLDATGVDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSGLCQP 240

Query: 241 LLDMKFLKTTEAGEQADVSEWVAEYIREKAPELLNLITCSEVFDSSRGGAVKMCREMDVP 300
           L+D KF+K T+ GEQ DV+EW+  Y+REKAPE+LNL+ CSEVFDSS GGA+KMC +M VP
Sbjct: 241 LMDFKFMKLTDNGEQKDVTEWILGYMREKAPEMLNLMACSEVFDSSGGGAMKMCNQMGVP 300

Query: 301 FLGKVPLDPQLCKAAEEGRSCFGDKKCGVSASALKKIIEKLIQNQGFS 349
           FLG+VPLDPQLCKAAEEGRSCF DK C VSA ALKKIIEKLI+  G S
Sbjct: 301 FLGRVPLDPQLCKAAEEGRSCFADKDCVVSAPALKKIIEKLIEANGLS 348

[Close]

BLAST of Lsi05G003800 vs. NCBI nr
Match: gi|595937573|ref|XP_007215600.1| (hypothetical protein PRUPE_ppa007759mg [Prunus persica])

HSP 1 Score: 633.3 bits (1632), Expect = 2.6e-178
Identity = 300/356 (84.27%), Postives = 332/356 (93.26%), Query Frame = 1


Query: 1   MENSEIPENANENCPGPQSESAGKSDACQGCPNQSICATAPKGPDPDLVSIAERMATVKH 60
           MEN EIPENANE+CPGPQS+SAGKSDAC+GCPNQ ICATAPKGPDPD+V+IAERMATVKH
Sbjct: 1   MENGEIPENANEHCPGPQSDSAGKSDACEGCPNQQICATAPKGPDPDVVAIAERMATVKH 60

Query: 61  KILILSGKGGVGKSTFSTQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLG 120
           KIL+LSGKGGVGKSTFS QLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQ++HQSNLG
Sbjct: 61  KILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQEIHQSNLG 120

Query: 121 WSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGT 180
           WSPVY+ESNLGVMSIGFMLP PD+AVIWRGPRKN LIKQFL+DVYWGELDFLVVDAPPGT
Sbjct: 121 WSPVYVESNLGVMSIGFMLPEPDDAVIWRGPRKNALIKQFLRDVYWGELDFLVVDAPPGT 180

Query: 181 SDEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSSLCQP 240
           SDEHISIVQCL+A  IDGAIIVTTPQQVSLIDVRKEVSFCKKVGV+VLG+VENMS LCQP
Sbjct: 181 SDEHISIVQCLDAGSIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVQVLGIVENMSGLCQP 240

Query: 241 LLDMKFLKTTEAGEQADVSEWVAEYIREKAPELLNLITCSEVFDSSRGGAVKMCREMDVP 300
           ++D KFL+ TEAGEQ DV+EW  E+++E+APE L++I C+EVFDSSRGGA KMCREM VP
Sbjct: 241 VMDFKFLRMTEAGEQIDVTEWAREHLKERAPEFLSIIACTEVFDSSRGGAEKMCREMGVP 300

Query: 301 FLGKVPLDPQLCKAAEEGRSCFGDKKCGVSASALKKIIEKLIQNQGFSTMSIDAKA 357
           FLGKVPLDPQLCKAAEEGRSCF D+KCGVSA ALKK+I+K+++NQG S M +D  A
Sbjct: 301 FLGKVPLDPQLCKAAEEGRSCFVDQKCGVSAPALKKVIDKMMENQGLSRMLVDNNA 356

[Close]

BLAST of Lsi05G003800 vs. NCBI nr
Match: gi|645250373|ref|XP_008231181.1| (PREDICTED: cytosolic Fe-S cluster assembly factor NBP35-like [Prunus mume])

HSP 1 Score: 629.0 bits (1621), Expect = 5.0e-177
Identity = 300/356 (84.27%), Postives = 330/356 (92.70%), Query Frame = 1


Query: 1   MENSEIPENANENCPGPQSESAGKSDACQGCPNQSICATAPKGPDPDLVSIAERMATVKH 60
           MEN EIPENANE+CPGPQS+SAGKSDAC+GCPNQ ICATAPKGPDPD+V+IAERMATVKH
Sbjct: 1   MENGEIPENANEHCPGPQSDSAGKSDACEGCPNQQICATAPKGPDPDVVAIAERMATVKH 60

Query: 61  KILILSGKGGVGKSTFSTQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLG 120
           KIL+LSGKGGVGKSTFS QLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQ++HQSNLG
Sbjct: 61  KILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQEIHQSNLG 120

Query: 121 WSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGT 180
           WSPVY+ESNLGVMSIGFMLP PD+AVIWRGPRKN LIKQFLKDVYWGELDFLVVDAPPGT
Sbjct: 121 WSPVYVESNLGVMSIGFMLPEPDDAVIWRGPRKNALIKQFLKDVYWGELDFLVVDAPPGT 180

Query: 181 SDEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSSLCQP 240
           SDEHISIVQ L+A  IDGAIIVTTPQQVSLIDVRKEVSFCKKVGV+VLG+VENMS LCQP
Sbjct: 181 SDEHISIVQYLDAGSIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVQVLGIVENMSGLCQP 240

Query: 241 LLDMKFLKTTEAGEQADVSEWVAEYIREKAPELLNLITCSEVFDSSRGGAVKMCREMDVP 300
           ++D KFL+ TEAGEQ DV+EW  E+++E+APE L+ I C+EVFDSSRGGA KMCREM VP
Sbjct: 241 VMDFKFLRMTEAGEQIDVTEWAREHLKERAPEFLSTIACTEVFDSSRGGAEKMCREMGVP 300

Query: 301 FLGKVPLDPQLCKAAEEGRSCFGDKKCGVSASALKKIIEKLIQNQGFSTMSIDAKA 357
           FLGKVPLDPQLCKAAEEGRSCF D+KCGVSA ALKK+I+K+++NQG S M +D  A
Sbjct: 301 FLGKVPLDPQLCKAAEEGRSCFVDQKCGVSAPALKKVIDKMMENQGLSRMLVDNNA 356

The following BLAST results are available for this feature:

BLAST of Lsi05G003800 vs. Swiss-Prot


Match Name	E-value	Identity	Description
NBP35_ARATH	5.0e-161	80.17	Cytosolic Fe-S cluster assembly factor NBP35 OS=Arabidopsis thaliana GN=NBP35 PE...	[more]
NUBP1_DICDI	5.4e-99	56.45	Cytosolic Fe-S cluster assembly factor NUBP1 homolog OS=Dictyostelium discoideum...	[more]
NUP1A_XENLA	1.3e-97	52.35	Cytosolic Fe-S cluster assembly factor nubp1-A OS=Xenopus laevis GN=nubp1-A PE=2...	[more]
NUBP1_NEMVE	1.1e-96	52.34	Cytosolic Fe-S cluster assembly factor NUBP1 homolog OS=Nematostella vectensis G...	[more]
NUBP1_DANRE	9.6e-96	52.35	Cytosolic Fe-S cluster assembly factor nubp1 OS=Danio rerio GN=nubp1 PE=2 SV=2	[more]

BLAST of Lsi05G003800 vs. TrEMBL


Match Name	E-value	Identity	Description
M5WSX8_PRUPE	1.8e-178	84.27	Cytosolic Fe-S cluster assembly factor NBP35 OS=Prunus persica GN=NBP35 PE=3 SV=...	[more]
A0A140B4A8_PRUPE	1.8e-178	84.27	Cytosolic Fe-S cluster assembly factor NBP35 OS=Prunus persica var. persica GN=N...	[more]
F6I5M0_VITVI	1.7e-176	85.92	Cytosolic Fe-S cluster assembly factor NBP35 OS=Vitis vinifera GN=NBP35 PE=3 SV=...	[more]
A0A0S3RQ06_PHAAN	1.7e-176	85.88	Cytosolic Fe-S cluster assembly factor NBP35 OS=Vigna angularis var. angularis G...	[more]
A0A0L9V1F3_PHAAN	1.7e-176	85.88	Cytosolic Fe-S cluster assembly factor NBP35 OS=Phaseolus angularis GN=NBP35 PE=...	[more]

BLAST of Lsi05G003800 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G50960.1	2.8e-162	80.17	nucleotide binding protein 35	[more]
AT4G19540.1	2.2e-34	44.21	IND1(iron-sulfur protein required for NADH dehydrogenase)-like	[more]
AT3G24430.1	4.4e-30	39.44	ATP binding	[more]
AT5G24020.1	1.4e-07	25.27	septum site-determining protein (MIND)	[more]

BLAST of Lsi05G003800 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449453858\|ref\|XP_004144673.1\|	2.5e-197	96.35	PREDICTED: cytosolic Fe-S cluster assembly factor NBP35 [Cucumis sativus]	[more]
gi\|659083114\|ref\|XP_008442190.1\|	7.4e-197	96.07	PREDICTED: cytosolic Fe-S cluster assembly factor NBP35 [Cucumis melo]	[more]
gi\|1021559407\|ref\|XP_016170986.1\|	2.0e-178	86.78	PREDICTED: cytosolic Fe-S cluster assembly factor NBP35 [Arachis ipaensis]	[more]
gi\|595937573\|ref\|XP_007215600.1\|	2.6e-178	84.27	hypothetical protein PRUPE_ppa007759mg [Prunus persica]	[more]
gi\|645250373\|ref\|XP_008231181.1\|	5.0e-177	84.27	PREDICTED: cytosolic Fe-S cluster assembly factor NBP35-like [Prunus mume]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005524	ATP binding
GO:0051539	4 iron, 4 sulfur cluster binding
GO:0051536	iron-sulfur cluster binding

Vocabulary: **Biological Process**
Term	Definition
GO:0016226	iron-sulfur cluster assembly

Vocabulary: **INTERPRO**
Term	Definition
IPR027417	P-loop_NTPase
IPR019591	Mrp/NBP35_ATP-bd
IPR000808	Mrp_CS

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016226	iron-sulfur cluster assembly
biological_process	GO:0071577	zinc II ion transmembrane transport
cellular_component	GO:0005829	cytosol
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0042803	protein homodimerization activity
molecular_function	GO:0005385	zinc ion transmembrane transporter activity
molecular_function	GO:0051536	iron-sulfur cluster binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G003800.1	Lsi05G003800.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000808	Mrp, conserved site	PROSITE	PS01215	MRP	coord: 166..182 scor
IPR019591	Mrp/NBP35 ATP-binding protein	HAMAP	MF_02040	Mrp_NBP35	coord: 53..342 score: 35
IPR019591	Mrp/NBP35 ATP-binding protein	PANTHER	PTHR23264	NUCLEOTIDE-BINDING PROTEIN NBP35 YEAST -RELATED	coord: 1..241 score: 4.0E-177coord: 279..343 score: 4.0E
IPR019591	Mrp/NBP35 ATP-binding protein	PFAM	PF10609	ParA	coord: 279..341 score: 9.0E-7coord: 58..241 score: 7.0
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	G3DSA:3.40.50.300		coord: 50..240 score: 1.4E-104coord: 275..340 score: 1.4E
IPR027417	P-loop containing nucleoside triphosphate hydrolase	unknown	SSF52540	P-loop containing nucleoside triphosphate hydrolases	coord: 45..242 score: 1.63E-51coord: 277..321 score: 1.63

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi05G003800	Silver-seed gourd	carlsiB435
Lsi05G003800	Silver-seed gourd	carlsiB700
Lsi05G003800	Cucumber (Chinese Long) v3	cuclsiB231
Lsi05G003800	Watermelon (97103) v2	lsiwmbB329
Lsi05G003800	Watermelon (97103) v2	lsiwmbB340
Lsi05G003800	Wax gourd	lsiwgoB447
Lsi05G003800	Cucumber (Gy14) v1	cgylsiB004
Lsi05G003800	Cucurbita maxima (Rimu)	cmalsiB249
Lsi05G003800	Cucurbita maxima (Rimu)	cmalsiB440
Lsi05G003800	Cucurbita moschata (Rifu)	cmolsiB237
Lsi05G003800	Cucurbita moschata (Rifu)	cmolsiB430
Lsi05G003800	Cucurbita pepo (Zucchini)	cpelsiB470
Lsi05G003800	Cucurbita pepo (Zucchini)	cpelsiB511
Lsi05G003800	Wild cucumber (PI 183967)	cpilsiB210
Lsi05G003800	Cucumber (Chinese Long) v2	culsiB212
Lsi05G003800	Melon (DHL92) v3.5.1	lsimeB368
Lsi05G003800	Watermelon (Charleston Gray)	lsiwcgB332
Lsi05G003800	Watermelon (97103) v1	lsiwmB361
Lsi05G003800	Cucumber (Gy14) v2	cgyblsiB199
Lsi05G003800	Melon (DHL92) v3.6.1	lsimedB399