Lsi05G003640 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi05G003640
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionGlutathione S-transferase
Locationchr05 : 4609965 .. 4616252 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTAATTTGGGCAAAAATTTGTGTCATCCCTACAAATTTCCCAAAAATTAGGAGAATTTTGCCAAAATTGTTCTCCCACTTTGGAGGCAAAGAATTGTTCGTGTACATTTTCCAACGGCATCCATTCGCCATTTGTTCTCTCGCGGAAACACAAACCTCATCTCCAACCCCTTCTTTCTATCACACGATCACTAGCAAATCCGTCTCCATTGCAAGAATTCAAAGATAAGAAAGAACTAGACTTTCACTCCAATGGCGACGCTAATGTCGGGCTCCAGAATTCAACCAGCAGCTTCTGTTCTTTCCTCCTCCATCAAGCGCCACATGGGTTTTAATTCCCACCTTCGTTCCAATTGCTCGCTTTCTCCTTTTCACCATTCTTCATTCTTCTTCAGGCACTCTGGACCTAGACGTTCTCTCTCTGTTTCCATGTCCGTTACCCCTCTTGAAGCCTGCGTCAAAGCTTCCACTACCGTACCCAACAAGCTCGGCGACTGTAATTTCTTCTTTCATTTCTTCTGTTTTCTGTTTTTTTTTTTCGATTAAGTAAGATAAAGTTAATTTCCTAAAAGTTTGTTGGTTTTCTTTATTTCCTGGTTGGAATTGATGTGTTTTGAGAAATGACGATAATATGATGGAATGCCTTTCACTGAACGTGAGAATTGTTGAAATTTATATGTATTTAACTTCAATTGGTCTATTTAGAATTATGTGAGTGTGACATTGATTGTAACCGGAGAAATATATTCATAGCCAAGAGAAGGGCGAAAGATAATTATCAAGTTGTTCGAGTTACTTGGACCTTTCTCTTGTTTTGGAGTGAAATCACTCTGAAACTTGCTTTTAACCATTCCAAATTAATTTTGATGATATGAATGTCCCGTTTAAAAATGTGAACATTAAATTGATTTTGAATGGCCAAAGTGAGCTTAGTTCAATGATAATTGGCATGCACTCCCTCCCTCCCTTGAGATCGGAGATTCAATCACCCATTTTCACAGTTGTAGATTTTCCAAACATGTAATCAAGCATAAGCCAACCAAACTTTTTCACGGCCTATCTCCATTTCTTCACTTATAACCAAGTTTTTGACTATGGAGAATTACCCCTTAATACCCTAATAACATTTCTTATTCTAATGACAATCCTAACAGTCATACGTAAAGCGCATAAGTTTGATGTAGTAGTATATGCCCTCAATTTGGTTTTAGTTATCAAATATTTTCATTCTTTGCAGGCCCTTTTTGTCAAAGGGTATTACTGACTTTGGAGGAAAAACATCTACCCTATGACTTAAAGTTGGTAGACATGTCGAACAAGCCAGAATGGTAATTATCTAATTTGATCTCTTTGTGAAAAAATAGTTTTTGTATAAATAGTTTGATATGAACCATTTGATGTATCAGAAATCCTTTTTTTCTTTTTTTCTTTGATAAACAGAGGAAGTGCTATATTTATGCCTTTTAAGTGTGCAAGTCTTTGAACTTTTTATCTGATTGTCTGTTCCTCTTTTTCTAATTACTTCTTTTGAGTCCAGGTTCTTAAAAATAAATTCAGAGGGCAAAGTTCCCGTTGTTAAATTTGATGAGCAGTGGGTTGCAGATTCAGATATTATAACACAAACACTGGAAGAGAAGTACCCAAATCCACCTTTAGCAACCCCTCCTGACAAATCTTCAGTGTATGTTCTATGTTCTATAATTGCCTCAATTTCAAAATTTCCTAGTGGTTGTAAGGTCAATTATATGACACAATTATACTAATAGTTTAACCAAACATGCTCATCAACGTACAAACTGGTGTTCAGATCGTAGTTCTGTAAAGTGTAATGTAAGATTTGATGATATCTTGTAAACTGCAACATTCAAACAAAATAAAAAGGGAAAACTAAATCTTTAGGCCACCATTTGGTAACTATTTGAGTTTTGAAAATTGTTGCTTGTTTTCTCACAATTCCGTTACCATGGTTTTCATTTTTCCAAGTAAACATATGAATTCTTGCTAAATTCCAAAGGCAAAAACAAGTTTTCAAAAACTACGGCCCCGTGTGGTAACCATTTCGTTTTTGGTTTTTGGTTTTTGAAAATTAAGCTTATTTCCTCATATTTTCTTACCATGGTTTGCATCTTTCTTGAGTACAAGAGTTGAATTCTAGCCAAATTCCAAAAACAACAAGTTTTTAAAAACTACCTTTTCTAGTTTTCAAAATTTAGCTTGGTTTTTGAAAACATGGGTAGAAAGTAGATAAGAAAGTAAGACATTTAAAGGTGAGAAGGGTGTTTGTCTTGTAGGCTTAATTTTCAAAAACTAAAAACCAAAAACCAAAAACCAAATGGTTATCAAACAGCCCATACTTTTTTTAGTTTTCAAAACTTGGCTTGGATTTTGAAAACACCTTTAAAAAGTAGACATCAAAACAAAGTAACACATAGATAGAAGTAGTGTATGCAAGCTTAATTTTCAAAAACCAAATGGTTATCAAACGAGATGTATATCTTATATTTTCATGTGGTTTCTTTCTTCGTTGATTATTAGTAGTTTGGAAAACAAAAGAAAACACAACTTTACTTTCAAAACAGTGTTTCAAGGATAAAGCAACAGCGAATAGTACTGTATTATCAAAGAGAAGTTATGTTTCAAGTATAAATAGAAAACAGTCTTTGAGAGTAGGGATCTTCAAACATGACATGAGGCTGTAAGCCTCTATGTATGTTCATTAAATTGAAAAGGAATTTTTGCATTCATGGATTTGAGTTGAATGAGAGTTTTTCGAAGATTCATTTTCTCCTTTTGGAATCTCATATTTCCTTGTCTTCGGTTAGTGTGGGGAATTTATACAATAAGTGTTTAATACAAAGATGAGAATTTTGTTGGTTATATGTTTCATATTTCATCTCCAAATTTATCAAATGATTAATATATTTACTGGCAGCTATTATAATATTTTTCCTGCAGAGGATCAAAGATTTTCTCCACATTTATTGGTTTTCTAAAGAGCAAGGATCCTAATGATGGAAAAGAACAAGTATTGCTTAGCGAGCTAAGTTCTTTCAATGATCACATCAAAGAGAATGTAAGATCTTTTTCTTCTGTATATTTCTTTTTAAATGAAATATCATACCATACAGGGGATGAACAAAGAATGAATCACAGAATGTTGAATGCATTCTTAGTAAATAATCTGTACTAAATTTTTATATATTTGGTTAAAGAAGAAACATAAATGGCTAAATGCATTCATCATTATCTCACATCACCACTGGCAAGCTCTGGGACATGTGGAAGTGTCTCTATTTTTTAAATTCTGTGATGAAAACTTCACAGAACCCTTTTCCAGCATTTGGTCTCTCTCACATCAATTGCAGTTTAATGTTTTTCCCCAAGTTTCTGAATTTTTTGCTGGCAGTACCACAAAATGATAGAAGTAGTAAGGCACCATTTCTTTTTCATGAATTGACATGCATGTCATAGTCCTGATTAAGCTTATTCCAGTTACAAGTTTACAATCACCTCTTGTTTCATATGTAATGAGTTGATAGATGACTATCTTAGATGGACTTGACCTTATCTTTCTCCTTCTAAATGACTTGGATCATATTTGATTCTGTACACTGTTCGGCTTTTGTTTGAGTTAAGTTACCATTGCTATTAATTCCTTTATTTCACTACTCGATGTTCTTGAATGGAATGAGACATGTTGCTGATAGGAACCAATATACAATCAACTGTATTGAAAGGTAAATGGTGAAAACTTACAAGATAGCCAAGTAATTTGAATAACTTGGACCCTCGTCTCTTGAGATCCCTATCAAGGCCTACTTCTCTCACCAAAATATTTCTTCCTTTCTTCATTCCCTAGCTAATTACTGACCAATGAGCATAACAAACTCGAGTTTTTTTGTATGAATGATCTTTTTAGAAAGGCCTTTATGATTGATGACCCATCCCTCAAAAACTTATGAAACCTAACCTTCTGTTTACATCAGAATGCACTACCGCATTTTATGATGTATCGCATTTAGGATGGGCATCTCTATTGTAGCTCTAACGCGATGGCAATCTCTATCGCAATCTTACTTTTCTTTCACTCAAGCACAACAAGCTGCAACAAACTGTGAGAGAAATTGGAAAAACACAACAAACTTTTATAACATATCGCGTTAGGAATGACCATTTGTAGTCCTAATGCGATGAGCATCCCTATCTCAATCTTCATTTTCTCACAAAAAAAGAAAAAAAAAACACAACTGTGAGAAAAATGGGGAAAACACAAATGTCCATGGCCATCATGTTAGGGTTACAATTGATATGATCATCCCTAATGCGATACGTTATAAAATGTGGTAGTGTGCTCTGACGTAAGTAGAAGGTTAGATTTCTTACATTGGTGGGGGCGGGTCATGGATCATAAAGGCGCTTGTGAATGAGTCATCCGTACAAAAAAACTCTAACAAACTCTCTAGCTAATTACTAATATACCCTTAATACTTTAATGACACCCTATTAGCATTACTGTCAGTTGCATATGCCTACATCTAAACTAAAGAAAAAATGTATAAAAATCATAAAAATGTAAATTCTATCCATTGAACTTTCTTAATTAATATTATAAAGGTAATTATCATCATTACTTATAAGAACAACTTATTCTTTAATTTTTTGTCATCAATGTTTTCATTGCTTTGGTAATAGAAGTATAATGGGCTAAATTAACCACTTGTGGTGTAGTGGCATTGAATACATAGATGCCTTTTATATTATTGATGAACGTGGCAAATGAATAATTATTCTTCTCATGATCCATTTCAAGTCAAGAGGCTTGAAGCATGTGTTTTCATGTAGGCTTAAGTCTCAAATGATCCAAAAAGCTCATAAGACTCGAGGTTAAAAATGTGAGTCTAGCTTTAAGGTGAGCCTCAAGGTGCAAGCTTCAAAAATCTTTTAAAACAGTGTCATTATCAATCTCTCCCCAGCACGAGTGGCAATGCATGAAAATAAGTAAATATCGGATGCTCTTTTGTGACTTACCATTTTATATCTCTCTTTTGAACAGGGCCCTTTTATCAATGGGAAAGAGATTTCTGCTGCAGACCTGTCACTTGGTCCAAAGTTGTACCATCTAGAAATTGCATTGGGTCACTATAAGAACTGGTCTGTTCCAGACAGTCTACCTTACGTTAAATCTTACATGAAGGTACAACTGAACTTTTAGCTAAACTGTGATTCATGGCCCATGATTTTCATCGTGCTTACAGCTTTTGACAACTGTTGTTTAGAGCTATTTTTATAAGCTAAATCGGAATACATTTTAAAATATAATGGTACGATACACTTGAAACCACTATTCATCCGCTCATGTTGTTCAAAGAATGAAAACTTCAACTATTCTCAAAGTTAGTCCACCCATGAATTTAAAGAGAATTAAGTTTTCAACGAAATAGTTTCTAAAAGACAGTGCAACAGTCTTTAAACACAACTCCAGAGTGGGACCATAGTTTCTGTTAGGTCTGCTGTAATTCATTATGAAAATGCATTTTTCTTTTAATGTTGCCTCCACTATCATTTAGAACAGGAGCTTACACATTATCCAAAAAATTTCACAACTAATAATGTCTGAATTCTAGATTATACCTTCATGTTTATTGAATAGTTGTGTTTAACAGCCTGCTATATGATAATGGAAAAACAGGAGCTGTGCAAGATTGAAGTTCAAATCTTTTCATCCCACTTTACTTCATAGAATCATAATTGAATAGAGAGCTTAGGACTGAAGTGCAGGATAATGGAAAAACCTTAACATTGGATTAACATGCAATATTATCCTTGAATCAGTTTCTTAATGTAACTGAAGCTTTGCTCATTTGTTTTATTACTGGCTTCAATGTCTTAATTATGACTGATGACATTGTATCAGAGCATATATTCAAGAGAATCATTTGCAAAAACCCGTGCTCTGCCTGAGGATGTTATTGCTGGTTGGCGTCCGAAGGTCTTTGGTTAAAATCTTTTCTAGGCTGCTGAATCGTTTGGCATGAGATTCCACTGTCTTCAAAGGCAAGCGCTGTAGTCATTAAAATTTCTAACAATTATGATGCAATGAGCGTTTTTTCTCCTGCTTCTAGTCACACTCAAAGCTGTTTAGTAGGAAATGTTTAGTACATTATGTTTATGTCCAATACATCATGTAAATTTAACTTTTCTATGAAAGGAAGTCATCCAAAGGGCAAGTACTACTATCCCATGTAATATGAATTTATAAAGTACTTGTGATTGCTTTTTCGTTCTTTA

mRNA sequence

GTTAATTTGGGCAAAAATTTGTGTCATCCCTACAAATTTCCCAAAAATTAGGAGAATTTTGCCAAAATTGTTCTCCCACTTTGGAGGCAAAGAATTGTTCGTGTACATTTTCCAACGGCATCCATTCGCCATTTGTTCTCTCGCGGAAACACAAACCTCATCTCCAACCCCTTCTTTCTATCACACGATCACTAGCAAATCCGTCTCCATTGCAAGAATTCAAAGATAAGAAAGAACTAGACTTTCACTCCAATGGCGACGCTAATGTCGGGCTCCAGAATTCAACCAGCAGCTTCTGTTCTTTCCTCCTCCATCAAGCGCCACATGGGTTTTAATTCCCACCTTCGTTCCAATTGCTCGCTTTCTCCTTTTCACCATTCTTCATTCTTCTTCAGGCACTCTGGACCTAGACGTTCTCTCTCTGTTTCCATGTCCGTTACCCCTCTTGAAGCCTGCGTCAAAGCTTCCACTACCGTACCCAACAAGCTCGGCGACTGCCCTTTTTGTCAAAGGGTATTACTGACTTTGGAGGAAAAACATCTACCCTATGACTTAAAGTTGGTAGACATGTCGAACAAGCCAGAATGGTTCTTAAAAATAAATTCAGAGGGCAAAGTTCCCGTTGTTAAATTTGATGAGCAGTGGGTTGCAGATTCAGATATTATAACACAAACACTGGAAGAGAAGTACCCAAATCCACCTTTAGCAACCCCTCCTGACAAATCTTCAGTAGGATCAAAGATTTTCTCCACATTTATTGGTTTTCTAAAGAGCAAGGATCCTAATGATGGAAAAGAACAAGTATTGCTTAGCGAGCTAAGTTCTTTCAATGATCACATCAAAGAGAATGGCCCTTTTATCAATGGGAAAGAGATTTCTGCTGCAGACCTGTCACTTGGTCCAAAGTTGTACCATCTAGAAATTGCATTGGGTCACTATAAGAACTGGTCTGTTCCAGACAGTCTACCTTACGTTAAATCTTACATGAAGAGCATATATTCAAGAGAATCATTTGCAAAAACCCGTGCTCTGCCTGAGGATGTTATTGCTGGTTGGCGTCCGAAGGTCTTTGGTTAAAATCTTTTCTAGGCTGCTGAATCGTTTGGCATGAGATTCCACTGTCTTCAAAGGCAAGCGCTGTAGTCATTAAAATTTCTAACAATTATGATGCAATGAGCGTTTTTTCTCCTGCTTCTAGTCACACTCAAAGCTGTTTAGTAGGAAATGTTTAGTACATTATGTTTATGTCCAATACATCATGTAAATTTAACTTTTCTATGAAAGGAAGTCATCCAAAGGGCAAGTACTACTATCCCATGTAATATGAATTTATAAAGTACTTGTGATTGCTTTTTCGTTCTTTA

Coding sequence (CDS)

ATGGCGACGCTAATGTCGGGCTCCAGAATTCAACCAGCAGCTTCTGTTCTTTCCTCCTCCATCAAGCGCCACATGGGTTTTAATTCCCACCTTCGTTCCAATTGCTCGCTTTCTCCTTTTCACCATTCTTCATTCTTCTTCAGGCACTCTGGACCTAGACGTTCTCTCTCTGTTTCCATGTCCGTTACCCCTCTTGAAGCCTGCGTCAAAGCTTCCACTACCGTACCCAACAAGCTCGGCGACTGCCCTTTTTGTCAAAGGGTATTACTGACTTTGGAGGAAAAACATCTACCCTATGACTTAAAGTTGGTAGACATGTCGAACAAGCCAGAATGGTTCTTAAAAATAAATTCAGAGGGCAAAGTTCCCGTTGTTAAATTTGATGAGCAGTGGGTTGCAGATTCAGATATTATAACACAAACACTGGAAGAGAAGTACCCAAATCCACCTTTAGCAACCCCTCCTGACAAATCTTCAGTAGGATCAAAGATTTTCTCCACATTTATTGGTTTTCTAAAGAGCAAGGATCCTAATGATGGAAAAGAACAAGTATTGCTTAGCGAGCTAAGTTCTTTCAATGATCACATCAAAGAGAATGGCCCTTTTATCAATGGGAAAGAGATTTCTGCTGCAGACCTGTCACTTGGTCCAAAGTTGTACCATCTAGAAATTGCATTGGGTCACTATAAGAACTGGTCTGTTCCAGACAGTCTACCTTACGTTAAATCTTACATGAAGAGCATATATTCAAGAGAATCATTTGCAAAAACCCGTGCTCTGCCTGAGGATGTTATTGCTGGTTGGCGTCCGAAGGTCTTTGGTTAA

Protein sequence

MATLMSGSRIQPAASVLSSSIKRHMGFNSHLRSNCSLSPFHHSSFFFRHSGPRRSLSVSMSVTPLEACVKASTTVPNKLGDCPFCQRVLLTLEEKHLPYDLKLVDMSNKPEWFLKINSEGKVPVVKFDEQWVADSDIITQTLEEKYPNPPLATPPDKSSVGSKIFSTFIGFLKSKDPNDGKEQVLLSELSSFNDHIKENGPFINGKEISAADLSLGPKLYHLEIALGHYKNWSVPDSLPYVKSYMKSIYSRESFAKTRALPEDVIAGWRPKVFG
Homology
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BLAST of Lsi05G003640 vs. Swiss-Prot
Match: DHAR3_ARATH (Glutathione S-transferase DHAR3, chloroplastic OS=Arabidopsis thaliana GN=DHAR3 PE=1 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 1.5e-101
Identity = 182/272 (66.91%), Postives = 218/272 (80.15%), Query Frame = 1 

Query: 5   MSGSRIQPA--ASVLSSSIKRHMGFNSHLRSNCSLSPFHHSSFFFRHSGPRRSLSVSMSV 64
           M   R QP+  A VLS+S+ R  GF     S              +     R ++++ + 
Sbjct: 1   MISLRFQPSTTAGVLSASVSR-AGFIKRCGST-------------KPGRVGRFVTMATAA 60

Query: 65  TPLEACVKASTTVPNKLGDCPFCQRVLLTLEEKHLPYDLKLVDMSNKPEWFLKINSEGKV 124
           +PLE CVKAS T PNKLGDCPFCQ+VLLT+EEK++PYD+K+VD+SNKPEWFLKI+ EGKV
Sbjct: 61  SPLEICVKASITTPNKLGDCPFCQKVLLTMEEKNVPYDMKMVDLSNKPEWFLKISPEGKV 120

Query: 125 PVVKFDEQWVADSDIITQTLEEKYPNPPLATPPDKSSVGSKIFSTFIGFLKSKDPNDGKE 184
           PVVKFDE+WV DSD+ITQ LEEKYP PPLATPP+K+SVGSKIFSTF+GFLKSKD  DG E
Sbjct: 121 PVVKFDEKWVPDSDVITQALEEKYPEPPLATPPEKASVGSKIFSTFVGFLKSKDSGDGTE 180

Query: 185 QVLLSELSSFNDHIKENGPFINGKEISAADLSLGPKLYHLEIALGHYKNWSVPDSLPYVK 244
           QVLL EL++FND+IK+NGPFING++ISAADLSL PKLYH++IALGHYKNWSVPDSLP+VK
Sbjct: 181 QVLLDELTTFNDYIKDNGPFINGEKISAADLSLAPKLYHMKIALGHYKNWSVPDSLPFVK 240

Query: 245 SYMKSIYSRESFAKTRALPEDVIAGWRPKVFG 275
           SYM++++SRESF  TRA  EDVIAGWRPKV G
Sbjct: 241 SYMENVFSRESFTNTRAETEDVIAGWRPKVMG 258

[Close]
BLAST of Lsi05G003640 vs. Swiss-Prot
Match: DHAR2_ARATH (Glutathione S-transferase DHAR2 OS=Arabidopsis thaliana GN=DHAR2 PE=1 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 1.8e-73
Identity = 129/209 (61.72%), Postives = 162/209 (77.51%), Query Frame = 1 

Query: 65  LEACVKASTTVPNKLGDCPFCQRVLLTLEEKHLPYDLKLVDMSNKPEWFLKINSEGKVPV 124
           L+ CVK +   P+ LGDCPF QRVLLTLEEK LPY   L+++S+KP+WFL I+ EGKVPV
Sbjct: 3   LDICVKVAVGAPDVLGDCPFSQRVLLTLEEKKLPYKTHLINVSDKPQWFLDISPEGKVPV 62

Query: 125 VKFDEQWVADSDIITQTLEEKYPNPPLATPPDKSSVGSKIFSTFIGFLKSKDPNDGKEQV 184
           VK D +WVADSD+I   LEEKYP P L TPP+ +SVGSKIF  F+ FLKSKD NDG E+ 
Sbjct: 63  VKLDGKWVADSDVIVGLLEEKYPEPSLKTPPEFASVGSKIFGAFVTFLKSKDANDGSEKA 122

Query: 185 LLSELSSFNDHIK-ENGPFINGKEISAADLSLGPKLYHLEIALGHYKNWSVPDSLPYVKS 244
           L+ EL +  +H+K  +GPF+ G++I+A DLSL PKLYHLE+ALGHYKNWSVP+SL  V++
Sbjct: 123 LVDELEALENHLKTHSGPFVAGEKITAVDLSLAPKLYHLEVALGHYKNWSVPESLTSVRN 182

Query: 245 YMKSIYSRESFAKTRALPEDVIAGWRPKV 273
           Y K+++SRESF  T+A  E V+AGW  KV
Sbjct: 183 YAKALFSRESFENTKAKKEIVVAGWESKV 211

[Close]
BLAST of Lsi05G003640 vs. Swiss-Prot
Match: DHAR1_ARATH (Glutathione S-transferase DHAR1, mitochondrial OS=Arabidopsis thaliana GN=DHAR1 PE=1 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 3.7e-71
Identity = 124/209 (59.33%), Postives = 161/209 (77.03%), Query Frame = 1 

Query: 65  LEACVKASTTVPNKLGDCPFCQRVLLTLEEKHLPYDLKLVDMSNKPEWFLKINSEGKVPV 124
           LE CVKA+   P+ LGDCPF QR LLTLEEK L Y + L+++S+KP+WFL I+ +GKVPV
Sbjct: 3   LEICVKAAVGAPDHLGDCPFSQRALLTLEEKSLTYKIHLINLSDKPQWFLDISPQGKVPV 62

Query: 125 VKFDEQWVADSDIITQTLEEKYPNPPLATPPDKSSVGSKIFSTFIGFLKSKDPNDGKEQV 184
           +K D++WV DSD+I   LEEKYP+PPL TP + +SVGS IF TF  FLKSKD NDG E  
Sbjct: 63  LKIDDKWVTDSDVIVGILEEKYPDPPLKTPAEFASVGSNIFGTFGTFLKSKDSNDGSEHA 122

Query: 185 LLSELSSFNDHIK-ENGPFINGKEISAADLSLGPKLYHLEIALGHYKNWSVPDSLPYVKS 244
           LL EL +  +H+K  +GPFI G+ +SA DLSL PKLYHL++ALGH+K+WSVP+S P+V +
Sbjct: 123 LLVELEALENHLKSHDGPFIAGERVSAVDLSLAPKLYHLQVALGHFKSWSVPESFPHVHN 182

Query: 245 YMKSIYSRESFAKTRALPEDVIAGWRPKV 273
           YMK+++S +SF KT+   + VI+GW PKV
Sbjct: 183 YMKTLFSLDSFEKTKTEEKYVISGWAPKV 211

[Close]
BLAST of Lsi05G003640 vs. Swiss-Prot
Match: DHAR4_ARATH (Putative glutathione S-transferase DHAR4 OS=Arabidopsis thaliana GN=DHAR4 PE=5 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 3.7e-63
Identity = 118/213 (55.40%), Postives = 154/213 (72.30%), Query Frame = 1 

Query: 65  LEACVKASTTVPNKLGDCPFCQRVLLTLEEKHLPYDLKLVDMSNKPEWFLKINSEGKVPV 124
           +E CVKA++  P+ LGDCPF QR+LLTLE+K LPY   L+D+S KP+WFL I+ +GK+P+
Sbjct: 3   IEVCVKAASGAPDVLGDCPFGQRILLTLEDKKLPYKTHLIDVSLKPDWFLAISPKGKLPL 62

Query: 125 VKF--DEQWVADSDIITQTLEEKYPNPPLAT-PPDKSSVGSKIFSTFIGFLKSKD-PNDG 184
           VKF  DE WVADSD+I   +EEKYP P L T PP+ +SVGSKI   F+ FL SKD  NDG
Sbjct: 63  VKFDEDENWVADSDLIVGIIEEKYPEPSLVTFPPEFASVGSKIIGAFVMFLTSKDHANDG 122

Query: 185 KEQVLLSELSSFNDHIKEN-GPFINGKEISAADLSLGPKLYHLEIALGHYKNWSVPDSLP 244
            +  LL EL + + H+K + GPF+ G +++  DLSL PKLYHLE  LGH+ +W VP+SL 
Sbjct: 123 SDMALLDELEALDHHLKTHVGPFVAGDKVTVVDLSLAPKLYHLETTLGHFMDWCVPESLT 182

Query: 245 YVKSYMKSIYSRESFAKTRALPEDVIAGWRPKV 273
            V+ YMK ++S ESF KT+A  E +IA W PK+
Sbjct: 183 NVRDYMKVLFSLESFEKTKAAKEYLIASWAPKL 215

[Close]
BLAST of Lsi05G003640 vs. Swiss-Prot
Match: CLIC1_BOVIN (Chloride intracellular channel protein 1 OS=Bos taurus GN=CLIC1 PE=2 SV=3)

HSP 1 Score: 94.7 bits (234), Expect = 1.6e-18
Identity = 59/210 (28.10%), Postives = 108/210 (51.43%), Query Frame = 1 

Query: 78  KLGDCPFCQRVLLTLEEKHLPYDLKLVDMSNKPEWFLKINSEGKVPVVKFDEQWVADSDI 137
           K+G+CPF QR+ + L  K + +++  VD   + E   K+   G++P + +  +   D++ 
Sbjct: 20  KIGNCPFSQRLFMVLWLKGVTFNVTTVDTKRRTETVQKLCPGGQLPFLLYGTEVHTDTNK 79

Query: 138 ITQTLEE-----KYPNPPLATPPDKSSVGSKIFSTFIGFLKSKDP--NDGKEQVLLSELS 197
           I + LE      +YP    A  P+ ++ G  IF+ F  ++K+ +P  ND  E+ LL  L 
Sbjct: 80  IEEFLEAVLCPPRYPKLA-ALNPESNTAGLDIFAKFSAYIKNSNPALNDNLEKGLLKALK 139

Query: 198 SFNDHIKENGP-----------------FINGKEISAADLSLGPKLYHLEIALGHYKNWS 257
             ++++    P                 F++G E++ AD +L PKL+ +++    Y+ +S
Sbjct: 140 VLDNYLTSPLPDEVDETSAEDEGISQRKFLDGNELTLADCNLLPKLHIVQVVCKKYRGFS 199

Query: 258 VPDSLPYVKSYMKSIYSRESFAKTRALPED 264
           +PD    V  Y+++ Y+RE FA T   P+D
Sbjct: 200 IPDVFRGVHRYLRNAYAREEFAST--CPDD 226

[Close]
BLAST of Lsi05G003640 vs. TrEMBL
Match: A0A0H3U218_SIRGR (Dehydroascorbate reductase OS=Siraitia grosvenorii PE=2 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 2.0e-132
Identity = 239/274 (87.23%), Postives = 253/274 (92.34%), Query Frame = 1 

Query: 1   MATLMSGSRIQPAASVLSSSIKRHMGFNSHLRSNCSLSPFHHSSFFFRHSGPRRSLSVSM 60
           M TL+S SRIQPAA VLSSSIK+ +G NS+ RSN  +SP HHSS   R SG RR+LSVSM
Sbjct: 1   MVTLLSSSRIQPAAFVLSSSIKQ-LGINSYPRSNFLVSP-HHSSLCLRRSGARRTLSVSM 60

Query: 61  SVTPLEACVKASTTVPNKLGDCPFCQRVLLTLEEKHLPYDLKLVDMSNKPEWFLKINSEG 120
           S TPLE CVKAS TVPN+LGDCPFCQRVLLTLEEKHLPYDLKLVD++NKPEWFLKINSEG
Sbjct: 61  SATPLETCVKASITVPNRLGDCPFCQRVLLTLEEKHLPYDLKLVDLANKPEWFLKINSEG 120

Query: 121 KVPVVKFDEQWVADSDIITQTLEEKYPNPPLATPPDKSSVGSKIFSTFIGFLKSKDPNDG 180
           KVPV+KFDEQWVADSD+ITQTLEEKYPNPPLATPPDKSSVGSKIFSTFI F+KSKDPNDG
Sbjct: 121 KVPVIKFDEQWVADSDVITQTLEEKYPNPPLATPPDKSSVGSKIFSTFIAFVKSKDPNDG 180

Query: 181 KEQVLLSELSSFNDHIKENGPFINGKEISAADLSLGPKLYHLEIALGHYKNWSVPDSLPY 240
            EQ LLSELSSFND+IKENGPFINGKEISAADLSLGPKLYHLEIALGHYKNWSVPDSLPY
Sbjct: 181 TEQALLSELSSFNDYIKENGPFINGKEISAADLSLGPKLYHLEIALGHYKNWSVPDSLPY 240

Query: 241 VKSYMKSIYSRESFAKTRALPEDVIAGWRPKVFG 275
           VKSYMKSI+SRESFAKTRALPEDVIAGWRPKV G
Sbjct: 241 VKSYMKSIFSRESFAKTRALPEDVIAGWRPKVLG 272

[Close]
BLAST of Lsi05G003640 vs. TrEMBL
Match: A9PDJ0_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0005s14550g PE=2 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 8.2e-110
Identity = 202/277 (72.92%), Postives = 233/277 (84.12%), Query Frame = 1 

Query: 5   MSGSRIQP---AASVLSSSIKRHMGFNSHLRSNCSLSPFHHSSFFFRHSGPRRSLSVSMS 64
           MS +RIQP   AA VLSS+IK H G  S LR      P +HS+ F R    +R+L+VSMS
Sbjct: 1   MSTARIQPTVTAACVLSSTIKHHFGLTS-LRL-----PTNHSAAF-RRQRIKRNLTVSMS 60

Query: 65  VT----PLEACVKASTTVPNKLGDCPFCQRVLLTLEEKHLPYDLKLVDMSNKPEWFLKIN 124
            T    PLE CVKAS TVP+KLGDCPFCQRVLLTLEEK+LPYD+K VD+ NKPEWFLK+N
Sbjct: 61  ATSVSEPLEICVKASLTVPDKLGDCPFCQRVLLTLEEKNLPYDMKFVDLGNKPEWFLKLN 120

Query: 125 SEGKVPVVKFDEQWVADSDIITQTLEEKYPNPPLATPPDKSSVGSKIFSTFIGFLKSKDP 184
            +GKVPV+KF+E WV+DSD+ITQ LEEK+P+PPLA PP+K+SVGSKIFSTFIGFLKSKDP
Sbjct: 121 PDGKVPVIKFEENWVSDSDVITQALEEKFPDPPLAIPPEKASVGSKIFSTFIGFLKSKDP 180

Query: 185 NDGKEQVLLSELSSFNDHIKENGPFINGKEISAADLSLGPKLYHLEIALGHYKNWSVPDS 244
            DG EQ LL ELS+FNDHIKENGPFING+++SAADL+LGPKLYHLEIALGHYKNWSVP+S
Sbjct: 181 GDGTEQALLDELSAFNDHIKENGPFINGEKVSAADLALGPKLYHLEIALGHYKNWSVPES 240

Query: 245 LPYVKSYMKSIYSRESFAKTRALPEDVIAGWRPKVFG 275
           LPY+KSY+K I+SR+SF  TRALPEDVIAGWRPKV G
Sbjct: 241 LPYIKSYLKEIFSRDSFVNTRALPEDVIAGWRPKVMG 270

[Close]
BLAST of Lsi05G003640 vs. TrEMBL
Match: A0A059AFH6_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_J01595 PE=4 SV=1)

HSP 1 Score: 404.4 bits (1038), Expect = 1.1e-109
Identity = 200/273 (73.26%), Postives = 231/273 (84.62%), Query Frame = 1 

Query: 5   MSGSRIQPAASVLSSSIKRHMGFNSHLRSNCSLSPFHHSSFFFRHSGPRRSLSV---SMS 64
           MS ++I P ASVLSS+IK ++GF  HL S   L     S+F  R+   +R L +   S++
Sbjct: 1   MSSAKITPTASVLSSTIK-NLGFGLHLPSYTVLP----SNFKQRYGCSQRRLCIMAKSLA 60

Query: 65  VTPLEACVKASTTVPNKLGDCPFCQRVLLTLEEKHLPYDLKLVDMSNKPEWFLKINSEGK 124
             PLE C KAS T+PNKLGDCPFCQRVL+T+EEK LPYDLKLVD+ NKPEWFLKINSEGK
Sbjct: 61  SNPLEVCAKASVTIPNKLGDCPFCQRVLMTMEEKQLPYDLKLVDLGNKPEWFLKINSEGK 120

Query: 125 VPVVKFDEQWVADSDIITQTLEEKYPNPPLATPPDKSSVGSKIFSTFIGFLKSKDPNDGK 184
           VPVVK DE WVADSD+ITQ+LEEKYP+PPL TP +K+S+GSKIFSTFIGFLKSKD +DG 
Sbjct: 121 VPVVKLDENWVADSDVITQSLEEKYPDPPLKTPAEKASIGSKIFSTFIGFLKSKDSSDGT 180

Query: 185 EQVLLSELSSFNDHIKENGPFINGKEISAADLSLGPKLYHLEIALGHYKNWSVPDSLPYV 244
           EQ LL+ELSSFNDHIKENGPFINGKEISAADLSLGPKL+H+EIALGHYKNWSVPD+LP+V
Sbjct: 181 EQALLNELSSFNDHIKENGPFINGKEISAADLSLGPKLHHMEIALGHYKNWSVPDTLPFV 240

Query: 245 KSYMKSIYSRESFAKTRALPEDVIAGWRPKVFG 275
           KSYM+S++SR+SF KTRAL EDVIAGWRPKV G
Sbjct: 241 KSYMQSVFSRDSFVKTRALKEDVIAGWRPKVMG 268

[Close]
BLAST of Lsi05G003640 vs. TrEMBL
Match: J9WQY6_POPTO (Dehydroascorbate reductase OS=Populus tomentosa GN=DHAR1 PE=2 SV=2)

HSP 1 Score: 403.3 bits (1035), Expect = 2.4e-109
Identity = 203/277 (73.29%), Postives = 231/277 (83.39%), Query Frame = 1 

Query: 5   MSGSRIQP---AASVLSSSIKRHMGFNSHLRSNCSLSPFHHSSFFFRHSGPRRSLSVSMS 64
           MS +RIQP   AA VLSS+IK H G  S LR      P +HS+ F R    +R+L+VSMS
Sbjct: 1   MSTARIQPTVTAACVLSSTIKHHFGLTS-LRL-----PTNHSAAF-RRQRIKRNLTVSMS 60

Query: 65  VT----PLEACVKASTTVPNKLGDCPFCQRVLLTLEEKHLPYDLKLVDMSNKPEWFLKIN 124
            T    PLE CVKAS TVP+KLGDCPFCQRVLLTLEEK+LPYD+K VD+ NKPEWFLK+N
Sbjct: 61  ATSISDPLEICVKASLTVPDKLGDCPFCQRVLLTLEEKNLPYDMKFVDLGNKPEWFLKLN 120

Query: 125 SEGKVPVVKFDEQWVADSDIITQTLEEKYPNPPLATPPDKSSVGSKIFSTFIGFLKSKDP 184
            +GKVPV+KF+E WV+DSD+ITQ LEEK+P+PPLA PP+K+SVGSKIFSTFIGFLKSKDP
Sbjct: 121 PDGKVPVIKFEENWVSDSDVITQALEEKFPDPPLAIPPEKASVGSKIFSTFIGFLKSKDP 180

Query: 185 NDGKEQVLLSELSSFNDHIKENGPFINGKEISAADLSLGPKLYHLEIALGHYKNWSVPDS 244
            DG EQ LL ELS+ NDHIKENGPFING+ +SAADL+LGPKLYHLEIALGHYKNWSVP+S
Sbjct: 181 GDGTEQALLDELSALNDHIKENGPFINGERVSAADLALGPKLYHLEIALGHYKNWSVPES 240

Query: 245 LPYVKSYMKSIYSRESFAKTRALPEDVIAGWRPKVFG 275
           LPYVKSYMK I+SR+SF  TRALPEDVIAGWRPKV G
Sbjct: 241 LPYVKSYMKEIFSRDSFVNTRALPEDVIAGWRPKVVG 270

[Close]
BLAST of Lsi05G003640 vs. TrEMBL
Match: A0A061EFZ7_THECC (Dehydroascorbate reductase 1 isoform 1 OS=Theobroma cacao GN=TCM_019159 PE=4 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 9.4e-106
Identity = 193/268 (72.01%), Postives = 220/268 (82.09%), Query Frame = 1 

Query: 14  ASVLSSSIKRHMGFNSHLRSNCSLSPFHHSSFFFRHSGPR----RSLSVSM---SVTPLE 73
           AS LS S+K H+ F      N +L          R +G R    RSL+V+M   S TPLE
Sbjct: 5   ASALSCSLKHHLAFGLRFPQNHALC---------RPNGARITTSRSLAVTMAATSTTPLE 64

Query: 74  ACVKASTTVPNKLGDCPFCQRVLLTLEEKHLPYDLKLVDMSNKPEWFLKINSEGKVPVVK 133
            CVKAS T PNKLGDCPFCQRVLLT EEKHLPYD+KLVD++NKPEWFL+I+ EGKVPV K
Sbjct: 65  VCVKASVTTPNKLGDCPFCQRVLLTFEEKHLPYDMKLVDLANKPEWFLQISPEGKVPVAK 124

Query: 134 FDEQWVADSDIITQTLEEKYPNPPLATPPDKSSVGSKIFSTFIGFLKSKDPNDGKEQVLL 193
            DE+WV DSD+ITQ+LEEKYP+PPL TPP+K+SVGSKIFSTFIGFLKSKDP DG EQ LL
Sbjct: 125 LDEKWVPDSDVITQSLEEKYPDPPLVTPPEKASVGSKIFSTFIGFLKSKDPTDGTEQALL 184

Query: 194 SELSSFNDHIKENGPFINGKEISAADLSLGPKLYHLEIALGHYKNWSVPDSLPYVKSYMK 253
           +ELSSFND+IKENGPFING++ISAADLSLGPKLYHLEIALGHYK WSVPD+LPYVKSYM+
Sbjct: 185 NELSSFNDYIKENGPFINGEKISAADLSLGPKLYHLEIALGHYKKWSVPDTLPYVKSYME 244

Query: 254 SIYSRESFAKTRALPEDVIAGWRPKVFG 275
           +I+S +SF KTRA P+DVIAGWRPKV G
Sbjct: 245 TIFSMDSFVKTRASPDDVIAGWRPKVMG 263

[Close]
BLAST of Lsi05G003640 vs. TAIR10
Match: AT5G16710.1 (AT5G16710.1 dehydroascorbate reductase 1)

HSP 1 Score: 370.5 bits (950), Expect = 8.7e-103
Identity = 182/272 (66.91%), Postives = 218/272 (80.15%), Query Frame = 1 

Query: 5   MSGSRIQPA--ASVLSSSIKRHMGFNSHLRSNCSLSPFHHSSFFFRHSGPRRSLSVSMSV 64
           M   R QP+  A VLS+S+ R  GF     S              +     R ++++ + 
Sbjct: 1   MISLRFQPSTTAGVLSASVSR-AGFIKRCGST-------------KPGRVGRFVTMATAA 60

Query: 65  TPLEACVKASTTVPNKLGDCPFCQRVLLTLEEKHLPYDLKLVDMSNKPEWFLKINSEGKV 124
           +PLE CVKAS T PNKLGDCPFCQ+VLLT+EEK++PYD+K+VD+SNKPEWFLKI+ EGKV
Sbjct: 61  SPLEICVKASITTPNKLGDCPFCQKVLLTMEEKNVPYDMKMVDLSNKPEWFLKISPEGKV 120

Query: 125 PVVKFDEQWVADSDIITQTLEEKYPNPPLATPPDKSSVGSKIFSTFIGFLKSKDPNDGKE 184
           PVVKFDE+WV DSD+ITQ LEEKYP PPLATPP+K+SVGSKIFSTF+GFLKSKD  DG E
Sbjct: 121 PVVKFDEKWVPDSDVITQALEEKYPEPPLATPPEKASVGSKIFSTFVGFLKSKDSGDGTE 180

Query: 185 QVLLSELSSFNDHIKENGPFINGKEISAADLSLGPKLYHLEIALGHYKNWSVPDSLPYVK 244
           QVLL EL++FND+IK+NGPFING++ISAADLSL PKLYH++IALGHYKNWSVPDSLP+VK
Sbjct: 181 QVLLDELTTFNDYIKDNGPFINGEKISAADLSLAPKLYHMKIALGHYKNWSVPDSLPFVK 240

Query: 245 SYMKSIYSRESFAKTRALPEDVIAGWRPKVFG 275
           SYM++++SRESF  TRA  EDVIAGWRPKV G
Sbjct: 241 SYMENVFSRESFTNTRAETEDVIAGWRPKVMG 258

[Close]
BLAST of Lsi05G003640 vs. TAIR10
Match: AT1G75270.1 (AT1G75270.1 dehydroascorbate reductase 2)

HSP 1 Score: 277.3 bits (708), Expect = 1.0e-74
Identity = 129/209 (61.72%), Postives = 162/209 (77.51%), Query Frame = 1 

Query: 65  LEACVKASTTVPNKLGDCPFCQRVLLTLEEKHLPYDLKLVDMSNKPEWFLKINSEGKVPV 124
           L+ CVK +   P+ LGDCPF QRVLLTLEEK LPY   L+++S+KP+WFL I+ EGKVPV
Sbjct: 3   LDICVKVAVGAPDVLGDCPFSQRVLLTLEEKKLPYKTHLINVSDKPQWFLDISPEGKVPV 62

Query: 125 VKFDEQWVADSDIITQTLEEKYPNPPLATPPDKSSVGSKIFSTFIGFLKSKDPNDGKEQV 184
           VK D +WVADSD+I   LEEKYP P L TPP+ +SVGSKIF  F+ FLKSKD NDG E+ 
Sbjct: 63  VKLDGKWVADSDVIVGLLEEKYPEPSLKTPPEFASVGSKIFGAFVTFLKSKDANDGSEKA 122

Query: 185 LLSELSSFNDHIK-ENGPFINGKEISAADLSLGPKLYHLEIALGHYKNWSVPDSLPYVKS 244
           L+ EL +  +H+K  +GPF+ G++I+A DLSL PKLYHLE+ALGHYKNWSVP+SL  V++
Sbjct: 123 LVDELEALENHLKTHSGPFVAGEKITAVDLSLAPKLYHLEVALGHYKNWSVPESLTSVRN 182

Query: 245 YMKSIYSRESFAKTRALPEDVIAGWRPKV 273
           Y K+++SRESF  T+A  E V+AGW  KV
Sbjct: 183 YAKALFSRESFENTKAKKEIVVAGWESKV 211

[Close]
BLAST of Lsi05G003640 vs. TAIR10
Match: AT1G19570.1 (AT1G19570.1 dehydroascorbate reductase)

HSP 1 Score: 269.6 bits (688), Expect = 2.1e-72
Identity = 124/209 (59.33%), Postives = 161/209 (77.03%), Query Frame = 1 

Query: 65  LEACVKASTTVPNKLGDCPFCQRVLLTLEEKHLPYDLKLVDMSNKPEWFLKINSEGKVPV 124
           LE CVKA+   P+ LGDCPF QR LLTLEEK L Y + L+++S+KP+WFL I+ +GKVPV
Sbjct: 3   LEICVKAAVGAPDHLGDCPFSQRALLTLEEKSLTYKIHLINLSDKPQWFLDISPQGKVPV 62

Query: 125 VKFDEQWVADSDIITQTLEEKYPNPPLATPPDKSSVGSKIFSTFIGFLKSKDPNDGKEQV 184
           +K D++WV DSD+I   LEEKYP+PPL TP + +SVGS IF TF  FLKSKD NDG E  
Sbjct: 63  LKIDDKWVTDSDVIVGILEEKYPDPPLKTPAEFASVGSNIFGTFGTFLKSKDSNDGSEHA 122

Query: 185 LLSELSSFNDHIK-ENGPFINGKEISAADLSLGPKLYHLEIALGHYKNWSVPDSLPYVKS 244
           LL EL +  +H+K  +GPFI G+ +SA DLSL PKLYHL++ALGH+K+WSVP+S P+V +
Sbjct: 123 LLVELEALENHLKSHDGPFIAGERVSAVDLSLAPKLYHLQVALGHFKSWSVPESFPHVHN 182

Query: 245 YMKSIYSRESFAKTRALPEDVIAGWRPKV 273
           YMK+++S +SF KT+   + VI+GW PKV
Sbjct: 183 YMKTLFSLDSFEKTKTEEKYVISGWAPKV 211

[Close]
BLAST of Lsi05G003640 vs. TAIR10
Match: AT1G19550.1 (AT1G19550.1 Glutathione S-transferase family protein)

HSP 1 Score: 163.7 bits (413), Expect = 1.6e-40
Identity = 80/162 (49.38%), Postives = 104/162 (64.20%), Query Frame = 1 

Query: 111 EWFLKINSEGKVPVVKFDEQWVADSDIITQTLEEKYPNPPLATPPDKSSVGSKIFSTFIG 170
           E F  I+ +GKVPV+K D++WV DSD     LEEKYP+PPL TP + +SVGS IF     
Sbjct: 13  EMFWDISPQGKVPVLKIDDKWVTDSDATVGILEEKYPDPPLKTPAEFASVGSNIFEALEN 72

Query: 171 FLKSKDPNDGKEQVLLSELSSFNDHIKENGPFINGKEISAADLSLGPKLYHLEIALGHYK 230
            LKS D                       GPFI G+ +SA DLSL PKLYHL++ALGH+K
Sbjct: 73  HLKSHD-----------------------GPFIAGERVSAVDLSLAPKLYHLQVALGHFK 132

Query: 231 NWSVPDSLPYVKSYMKSIYSRESFAKTRALPEDVIAGWRPKV 273
           +WSVP+S P+V +YMK+++S +SF KT+   + VI+GW PKV
Sbjct: 133 SWSVPESFPHVHNYMKTLFSLDSFEKTKTEEKCVISGWAPKV 151

[Close]
BLAST of Lsi05G003640 vs. NCBI nr
Match: gi|449456235|ref|XP_004145855.1| (PREDICTED: glutathione S-transferase DHAR3, chloroplastic [Cucumis sativus])

HSP 1 Score: 508.4 bits (1308), Expect = 7.5e-141
Identity = 248/270 (91.85%), Postives = 257/270 (95.19%), Query Frame = 1 

Query: 5   MSGSRIQPAASVLSSSIKRHMGFNSHLRSNCSLSPFHHSSFFFRHSGPRRSLSVSMSVTP 64
           MSGSRIQPAASVLSSSIKRHMGF+ +L SN SLSPFHHSSF FR SGP RSLSVSMSV P
Sbjct: 1   MSGSRIQPAASVLSSSIKRHMGFSFYLPSNSSLSPFHHSSFSFRRSGPARSLSVSMSVAP 60

Query: 65  LEACVKASTTVPNKLGDCPFCQRVLLTLEEKHLPYDLKLVDMSNKPEWFLKINSEGKVPV 124
           LEACVKASTT+PNKLGDCPFCQRVLLTLEEKHLPYDLKLVD+SNKPEWFLKINSEGKVPV
Sbjct: 61  LEACVKASTTLPNKLGDCPFCQRVLLTLEEKHLPYDLKLVDLSNKPEWFLKINSEGKVPV 120

Query: 125 VKFDEQWVADSDIITQTLEEKYPNPPLATPPDKSSVGSKIFSTFIGFLKSKDPNDGKEQV 184
           VKFDEQW+ADSD+IT+TLEEKYPNPPL TPPDKSSVGSKIFSTFI FLKSKDPNDG EQ 
Sbjct: 121 VKFDEQWIADSDVITETLEEKYPNPPLVTPPDKSSVGSKIFSTFIAFLKSKDPNDGTEQA 180

Query: 185 LLSELSSFNDHIKENGPFINGKEISAADLSLGPKLYHLEIALGHYKNWSVPDSLPYVKSY 244
           LLSEL+SFNDHIKENGP INGKEISAADLSLGPKLYHLEIALGHYKNWSVPDSLPYVKSY
Sbjct: 181 LLSELTSFNDHIKENGPLINGKEISAADLSLGPKLYHLEIALGHYKNWSVPDSLPYVKSY 240

Query: 245 MKSIYSRESFAKTRALPEDVIAGWRPKVFG 275
           MKSI+SRESFAKTRALPEDVIAGWRPKV G
Sbjct: 241 MKSIFSRESFAKTRALPEDVIAGWRPKVLG 270

[Close]
BLAST of Lsi05G003640 vs. NCBI nr
Match: gi|659114326|ref|XP_008457014.1| (PREDICTED: glutathione S-transferase DHAR3, chloroplastic [Cucumis melo])

HSP 1 Score: 499.6 bits (1285), Expect = 3.5e-138
Identity = 245/270 (90.74%), Postives = 253/270 (93.70%), Query Frame = 1 

Query: 5   MSGSRIQPAASVLSSSIKRHMGFNSHLRSNCSLSPFHHSSFFFRHSGPRRSLSVSMSVTP 64
           MS SRIQPAASVLS+SIKRH GF+ HL S  S+SPFHHSSF FR SG  RSLSVSMSV P
Sbjct: 1   MSASRIQPAASVLSTSIKRHTGFSFHLPSYSSVSPFHHSSFSFRRSGLTRSLSVSMSVAP 60

Query: 65  LEACVKASTTVPNKLGDCPFCQRVLLTLEEKHLPYDLKLVDMSNKPEWFLKINSEGKVPV 124
           LEACVKASTT+PNKLGDCPFCQRVLLTLEEKHLPYDLKLVD+SNKPEWFLKINSEGKVPV
Sbjct: 61  LEACVKASTTLPNKLGDCPFCQRVLLTLEEKHLPYDLKLVDLSNKPEWFLKINSEGKVPV 120

Query: 125 VKFDEQWVADSDIITQTLEEKYPNPPLATPPDKSSVGSKIFSTFIGFLKSKDPNDGKEQV 184
           VKFDEQW+ADSD+ITQTLEEKYPNPPLATPPDKSSVG K FSTFI FLKSKDPNDG EQ 
Sbjct: 121 VKFDEQWIADSDVITQTLEEKYPNPPLATPPDKSSVGCKFFSTFIAFLKSKDPNDGTEQA 180

Query: 185 LLSELSSFNDHIKENGPFINGKEISAADLSLGPKLYHLEIALGHYKNWSVPDSLPYVKSY 244
           LLSELSSFNDHIKENGPFINGKEISAADLSLGPKLYHLEIALGHYKNWSVPDSLPYVKSY
Sbjct: 181 LLSELSSFNDHIKENGPFINGKEISAADLSLGPKLYHLEIALGHYKNWSVPDSLPYVKSY 240

Query: 245 MKSIYSRESFAKTRALPEDVIAGWRPKVFG 275
           MKSI+SRESFAKTRALPEDVIAGWRPKV G
Sbjct: 241 MKSIFSRESFAKTRALPEDVIAGWRPKVLG 270

[Close]
BLAST of Lsi05G003640 vs. NCBI nr
Match: gi|595333702|gb|AHM26862.1| (dehydroascorbate reductase [Siraitia grosvenorii])

HSP 1 Score: 479.9 bits (1234), Expect = 2.9e-132
Identity = 239/274 (87.23%), Postives = 253/274 (92.34%), Query Frame = 1 

Query: 1   MATLMSGSRIQPAASVLSSSIKRHMGFNSHLRSNCSLSPFHHSSFFFRHSGPRRSLSVSM 60
           M TL+S SRIQPAA VLSSSIK+ +G NS+ RSN  +SP HHSS   R SG RR+LSVSM
Sbjct: 1   MVTLLSSSRIQPAAFVLSSSIKQ-LGINSYPRSNFLVSP-HHSSLCLRRSGARRTLSVSM 60

Query: 61  SVTPLEACVKASTTVPNKLGDCPFCQRVLLTLEEKHLPYDLKLVDMSNKPEWFLKINSEG 120
           S TPLE CVKAS TVPN+LGDCPFCQRVLLTLEEKHLPYDLKLVD++NKPEWFLKINSEG
Sbjct: 61  SATPLETCVKASITVPNRLGDCPFCQRVLLTLEEKHLPYDLKLVDLANKPEWFLKINSEG 120

Query: 121 KVPVVKFDEQWVADSDIITQTLEEKYPNPPLATPPDKSSVGSKIFSTFIGFLKSKDPNDG 180
           KVPV+KFDEQWVADSD+ITQTLEEKYPNPPLATPPDKSSVGSKIFSTFI F+KSKDPNDG
Sbjct: 121 KVPVIKFDEQWVADSDVITQTLEEKYPNPPLATPPDKSSVGSKIFSTFIAFVKSKDPNDG 180

Query: 181 KEQVLLSELSSFNDHIKENGPFINGKEISAADLSLGPKLYHLEIALGHYKNWSVPDSLPY 240
            EQ LLSELSSFND+IKENGPFINGKEISAADLSLGPKLYHLEIALGHYKNWSVPDSLPY
Sbjct: 181 TEQALLSELSSFNDYIKENGPFINGKEISAADLSLGPKLYHLEIALGHYKNWSVPDSLPY 240

Query: 241 VKSYMKSIYSRESFAKTRALPEDVIAGWRPKVFG 275
           VKSYMKSI+SRESFAKTRALPEDVIAGWRPKV G
Sbjct: 241 VKSYMKSIFSRESFAKTRALPEDVIAGWRPKVLG 272

[Close]
BLAST of Lsi05G003640 vs. NCBI nr
Match: gi|694412206|ref|XP_009334436.1| (PREDICTED: glutathione S-transferase DHAR3, chloroplastic-like [Pyrus x bretschneideri])

HSP 1 Score: 409.5 bits (1051), Expect = 4.8e-111
Identity = 202/271 (74.54%), Postives = 229/271 (84.50%), Query Frame = 1 

Query: 4   LMSGSRIQPAASVLSSSIKRHMGFNSHLRSNCSLSPFHHSSFFFRHSGPRRSLSVSMSVT 63
           +++ ++I PAAS + SS  +H     HLRS  +   F  +    R  G  R+L+V+M V 
Sbjct: 1   MLTTAKIHPAASAVLSSTIKH-----HLRSPPNAVVFRTNPNSLRRRGTTRTLTVAM-VA 60

Query: 64  PLEACVKASTTVPNKLGDCPFCQRVLLTLEEKHLPYDLKLVDMSNKPEWFLKINSEGKVP 123
           PL+ C KASTTVPNKLGDCPFCQRVLLTLEEKHLPYDLKLVD+ NKPEWFLKINSEGKVP
Sbjct: 61  PLDVCAKASTTVPNKLGDCPFCQRVLLTLEEKHLPYDLKLVDLGNKPEWFLKINSEGKVP 120

Query: 124 VVKFDEQWVADSDIITQTLEEKYPNPPLATPPDKSSVGSKIFSTFIGFLKSKDPNDGKEQ 183
           VVK DE+WVADSDIITQ LEEKYP PPLATPP+K+SVGSKIFSTFIGFLKSKDP DG EQ
Sbjct: 121 VVKLDEKWVADSDIITQALEEKYPEPPLATPPEKASVGSKIFSTFIGFLKSKDPKDGTEQ 180

Query: 184 VLLSELSSFNDHIKENGPFINGKEISAADLSLGPKLYHLEIALGHYKNWSVPDSLPYVKS 243
            LL+ELSSFND++K+NGPFINGKE+SAADLSLGPKLYHLEIALGH+K+WS+PDSLPYVKS
Sbjct: 181 ALLNELSSFNDYLKDNGPFINGKEVSAADLSLGPKLYHLEIALGHFKDWSIPDSLPYVKS 240

Query: 244 YMKSIYSRESFAKTRALPEDVIAGWRPKVFG 275
           YMKSI+S +SF KT AL EDVIAGWRPKV G
Sbjct: 241 YMKSIFSLDSFVKTSALKEDVIAGWRPKVLG 265

[Close]
BLAST of Lsi05G003640 vs. NCBI nr
Match: gi|658053505|ref|XP_008362508.1| (PREDICTED: glutathione S-transferase DHAR3, chloroplastic-like [Malus domestica])

HSP 1 Score: 407.1 bits (1045), Expect = 2.4e-110
Identity = 205/272 (75.37%), Postives = 229/272 (84.19%), Query Frame = 1 

Query: 4   LMSGSRIQPAAS-VLSSSIKRHMGFNSHLRSNCSLSPFHHSSFFFRHSGPRRSLSVSMSV 63
           +++ ++I PAAS VLSSSIK H      LR   +   F  +    R  G  R+L+V+M V
Sbjct: 1   MLTTAKIHPAASAVLSSSIKHH------LRPPPNAVVFRTNPNSLRRRGTTRTLTVAM-V 60

Query: 64  TPLEACVKASTTVPNKLGDCPFCQRVLLTLEEKHLPYDLKLVDMSNKPEWFLKINSEGKV 123
            PL+ C KASTTVPNKLGDCPFCQRVLLTLEEKHLPYDLKLVD+ NKPEWFLKINSEGKV
Sbjct: 61  APLDVCAKASTTVPNKLGDCPFCQRVLLTLEEKHLPYDLKLVDLGNKPEWFLKINSEGKV 120

Query: 124 PVVKFDEQWVADSDIITQTLEEKYPNPPLATPPDKSSVGSKIFSTFIGFLKSKDPNDGKE 183
           PVVK DE+WVADSDIITQ LEEKYP P LATPP+K+SVGSKIFSTFIGFLKSKDP DG E
Sbjct: 121 PVVKLDEKWVADSDIITQALEEKYPEPXLATPPEKASVGSKIFSTFIGFLKSKDPKDGTE 180

Query: 184 QVLLSELSSFNDHIKENGPFINGKEISAADLSLGPKLYHLEIALGHYKNWSVPDSLPYVK 243
           Q LL+ELSSFND++KENGPFINGKE+SAADLSLGPKLYHLEIALGH+K+WS+PDSLPYVK
Sbjct: 181 QALLNELSSFNDYLKENGPFINGKEVSAADLSLGPKLYHLEIALGHFKDWSIPDSLPYVK 240

Query: 244 SYMKSIYSRESFAKTRALPEDVIAGWRPKVFG 275
           SYMKSI+S +SF KT AL EDVIAGWRPKV G
Sbjct: 241 SYMKSIFSLDSFVKTSALKEDVIAGWRPKVLG 265

The following BLAST results are available for this feature:
BLAST of Lsi05G003640 vs. Swiss-Prot
Match NameE-valueIdentityDescription
DHAR3_ARATH1.5e-10166.91Glutathione S-transferase DHAR3, chloroplastic OS=Arabidopsis thaliana GN=DHAR3 ... [more]
DHAR2_ARATH1.8e-7361.72Glutathione S-transferase DHAR2 OS=Arabidopsis thaliana GN=DHAR2 PE=1 SV=1[more]
DHAR1_ARATH3.7e-7159.33Glutathione S-transferase DHAR1, mitochondrial OS=Arabidopsis thaliana GN=DHAR1 ... [more]
DHAR4_ARATH3.7e-6355.40Putative glutathione S-transferase DHAR4 OS=Arabidopsis thaliana GN=DHAR4 PE=5 S... [more]
CLIC1_BOVIN1.6e-1828.10Chloride intracellular channel protein 1 OS=Bos taurus GN=CLIC1 PE=2 SV=3[more]
BLAST of Lsi05G003640 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0H3U218_SIRGR2.0e-13287.23Dehydroascorbate reductase OS=Siraitia grosvenorii PE=2 SV=1[more]
A9PDJ0_POPTR8.2e-11072.92Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0005s14550g PE=2 SV=1[more]
A0A059AFH6_EUCGR1.1e-10973.26Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_J01595 PE=4 SV=1[more]
J9WQY6_POPTO2.4e-10973.29Dehydroascorbate reductase OS=Populus tomentosa GN=DHAR1 PE=2 SV=2[more]
A0A061EFZ7_THECC9.4e-10672.01Dehydroascorbate reductase 1 isoform 1 OS=Theobroma cacao GN=TCM_019159 PE=4 SV=... [more]
BLAST of Lsi05G003640 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G16710.18.7e-10366.91 dehydroascorbate reductase 1[more]
AT1G75270.11.0e-7461.72 dehydroascorbate reductase 2[more]
AT1G19570.12.1e-7259.33 dehydroascorbate reductase[more]
AT1G19550.11.6e-4049.38 Glutathione S-transferase family protein[more]
BLAST of Lsi05G003640 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449456235|ref|XP_004145855.1|7.5e-14191.85PREDICTED: glutathione S-transferase DHAR3, chloroplastic [Cucumis sativus][more]
gi|659114326|ref|XP_008457014.1|3.5e-13890.74PREDICTED: glutathione S-transferase DHAR3, chloroplastic [Cucumis melo][more]
gi|595333702|gb|AHM26862.1|2.9e-13287.23dehydroascorbate reductase [Siraitia grosvenorii][more]
gi|694412206|ref|XP_009334436.1|4.8e-11174.54PREDICTED: glutathione S-transferase DHAR3, chloroplastic-like [Pyrus x bretschn... [more]
gi|658053505|ref|XP_008362508.1|2.4e-11075.37PREDICTED: glutathione S-transferase DHAR3, chloroplastic-like [Malus domestica][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005515protein binding
Vocabulary: INTERPRO
TermDefinition
IPR012336Thioredoxin-like_fold
IPR010987Glutathione-S-Trfase_C-like
IPR004045Glutathione_S-Trfase_N
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0098869 cellular oxidant detoxification
biological_process GO:0006749 glutathione metabolic process
biological_process GO:0019852 L-ascorbic acid metabolic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0010731 protein glutathionylation
biological_process GO:0009735 response to cytokinin
biological_process GO:0006461 protein complex assembly
biological_process GO:0008150 biological_process
cellular_component GO:0009941 chloroplast envelope
cellular_component GO:0009570 chloroplast stroma
cellular_component GO:0005739 mitochondrion
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005575 cellular_component
molecular_function GO:0045174 glutathione dehydrogenase (ascorbate) activity
molecular_function GO:0004364 glutathione transferase activity
molecular_function GO:0005515 protein binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G003640.1Lsi05G003640.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004045Glutathione S-transferase, N-terminalPFAMPF13417GST_N_3coord: 81..150
score: 1.9
IPR004045Glutathione S-transferase, N-terminalPROFILEPS50404GST_NTERcoord: 72..150
score: 14
IPR010987Glutathione S-transferase, C-terminal-likeGENE3DG3DSA:1.20.1050.10coord: 154..271
score: 5.8
IPR010987Glutathione S-transferase, C-terminal-likePROFILEPS50405GST_CTERcoord: 128..273
score: 9
IPR010987Glutathione S-transferase, C-terminal-likeunknownSSF47616GST C-terminal domain-likecoord: 145..272
score: 4.5
IPR012336Thioredoxin-like foldGENE3DG3DSA:3.40.30.10coord: 63..152
score: 6.6
IPR012336Thioredoxin-like foldunknownSSF52833Thioredoxin-likecoord: 81..149
score: 1.57
NoneNo IPR availablePANTHERPTHR11260GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAININGcoord: 47..274
score: 1.5E
NoneNo IPR availablePANTHERPTHR11260:SF171GLUTATHIONE S-TRANSFERASE DHAR3, CHLOROPLASTICcoord: 47..274
score: 1.5E
NoneNo IPR availablePFAMPF13410GST_C_2coord: 182..246
score: 1.