Lsi05G003300 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi05G003300
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	5'-AMP-activated protein kinase-related
Location	chr05 : 4218219 .. 4223011 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATGTTTGATCTTTGTGGCTGGGACCGTACCTCTAGGCATGTTATACTTGACAAACAGAATTTTCTTTTGACTGTCAAATGTTTGTAAATTCAGAGACCACCTTAAAGAAGTTGTATTTCTGGAAAGGAATATCTTGGATTTTCTCAGCTCTATCAGGGAGGCCGAGAGAAGTACTTTGTTTACATTGAGAAACAAGAGTGAAGGCTTTCAAATAGATCCACACAAAAGAAAGTCTTTCACCGAAGAGAGAAGAGGTCTAAATACATATGGCTTTTCATTTCTTTTTTGAGTCAAATGTTCTTTTGAGGAACTCCATTTCTTATTTTCTTGGTATTCTTTTCTGTGGCTAATGTTATGTTTACATTAGAATTTTCATATGTACTTTGATAGTTCATGATTTTCTTGTTCTTCATGATTTTCATGATTTTCGTATTTACTTTGATAGGAGCCTGCCAATATGGAGATTTGTGCTCGAAGGTACTTATTCTTTTCTATACGAGAAAATTGTTGTTATGAACCTCTTAATATCTTGTTGACTTGAGCTTCAGTTATAGATTGCTGGCTCTAATTTTTGTGCCCCTTCCTGAAAAGGTTTGATGATTATCTCTTCATCTGGAACTTACTTGAAGCAATGTATCAAGAAGAAAGCATGCTCTGTATTTTTCGTTTTCTCTTCTTTTTAAATTTTGTTTCAATCGAAAGGGGTTTAGGTTATCGTCTTTAATATGGATGTGATTAATGCAATTTTCCATTATGTCTATAATCATGAACAATTGAACATTATCTGTTAGTTCCAAATCATTATTTTCACTACGTTTGAAACTTTCTGAGAACCTGATGCCATTATTACTTATTTTATAATCTTGGTTAGGACTTGTCTGGACAACCAAGTTTTACTGAGTTCCAAGAATGCAAGAAAGTTAAAGATGGAAAACTTGAATAACCCTTCATGTAATATGGCAACCTGGAGCTTTAGGATGGGTTCTTGCAAGTTGATCCATTCTCATGAACCACCTGTAACCACACTTCCTCAAATGTATCGGATTCACAAAAGATATTCCTTGTCTGTCACATCAGAGGAATCTTCAATGCAATCAGAAGATCTTTCTACAGACGAAGAGGAAATTGTGAGTGCAGAGGAACTTCTGGCTCAACCCCTTAGTAGTGAACAGGTAAACGTAGACATTAAGTTCATAATTTAATGCGTTCGTGATACAGAATGAAGGATACTGCATTTATAATCTGCTGTTGTGACTGAGAATGAAATCAATTGCCACCTATTTCTTATTTTTGCTCGCTCAAAATCTTTCATGTAAAGTTTTAGCCTTCTTTTCCAAAACTCCTTCTTTTCCAAAACTTACAGTGTCCTCATTTTCACTATTTCTTTTAGCCTAAGTTATTTCTATCCTCAGTGCCGACTTTTCAACACAGTGTAGTGGTGTTTAGTGATTGATGCTCCTTTGCCATTTTGGAAAATAAAAGTTACTGGTTCTTGTCAGGCTTACATGATTTCCATACCTATCTTCAGAGAAGGTGGGGAATGTTGAGCGGGGAGATAAAAGAAAATCAACAGGGTTACCATTGTTTAGGAATTTAAAAGCTTCTGGTTTTTTGGAAAGACTTCAAGAATGTTAGTGACAGTGGGTGTCTATTGGGGAAGGATTTGGTATCATGGGTTCAAACTTTGGGCCACCTACTTACATCCTCAGTCTAACTTTGTAGGGTTAGACAAATTCTCTTTCTAAATTAGTTAAGGAATACTTAAGCTAATTTAGACAAGCTATGATTATCCAAAAAGGGAAAGTTTACATTTGTAGGAGAATAGAAAAATGGAAAAAAGAATAAAATACTGTAAAGGAACATATAGATATACTGAGCTTCAAAAAAATTGTCTCAGTATGAATGAATCTCTAAAAATTTTGGTATGCTCCATTCTTTTACTTACAATTTCTTGAATAGATTGTAGTGTTATGTAGACGAGAATCCTACTATTGCTGAAAACAAAACATAACAACTGATCGTGTAAAATATGTGAAGTGATCACAGCTTGGATAAACAAATAATGTTATGTAGAAAGCAATCAAAGCTGCATTGGCATTTCTTCAAAGCTTTCCTTCCATTCTGGAATGTTTCTTTCCGGAGAGCTTGATATCAAGCTTTTTTGTCTGTCAAAGATCTGAATGATATATAATTGACTACAGCTGAATGCATTACTGGCTGATTCAGAAAGAGAGAAGCTTGTCAAAAAGCTAAGTCACGCTAATCAGCAGAACAGACTTCTCAAACGACAGGTGTAAAGACATGATTTTTCTTTTTTTACTTTACAAGTATGTTAACCAAAGTTTTTACTCTTCATTATGCAGATGCAGACTCATTTTGCTATTATTATTTGTTTTTATGATCATGTTTTATGTTTACTATATCTTCTATTGTACTGGTTTTATATGGTGCAGATTAACTTAGAAACCAGAATCAAAATTCTATTAATAAATCTATTTGTCTCCAATTTATACTCAAATCTCGAATATTGGTCACAAAAATGTGCATATATTAACTTTTTAGTTAATTTTTATTTAATATGTGATAGGTTGAGTTATGAGTTTAGTTCCTCATCCATAGAACAATATTTCTTGACGTCCCTTTTTGCTCTGGTATTAATCTGTCCAGTCTTTTTTTCCTAATGTTAATTTGTCTTTCTGTTCTATGACCTATGTCAAGTAAATCATTTTTGTTGAGAATTATTAACAAAAGAATCCACCTGAAATAGATCGTTAGTCAAAGAAGAAATTCTGCTTCTTGCTTTCTTTTGTTCGATTAATGTCTGCAATAAATTAAGCTTTTCTATCAAGTTCATATTCCGTGGCATAATGACATCTTACTCTGAGGCATCTATATGTTTAGAACTCGTGCAATCATGTTTCTTTTCCCTTATCAGTTGTAAAATGTTCGAGTTAGACAGAGAGAAGAAAAACAAAATAATTCCTGAAAAGAGCTACAATATCTATCTATCCCTTGTAAAGCTTTCTTTATTATTTCAAGACAACTTTCCCATTAGATATATCTAAAGGCATTAAGCCACAAGGATTTGACATTTCGAATATTGAATCCTGTAATTTTTATATGTTGGCCCCTGGGAGTTGTAGATGTTTAGTGCGTTCTTATTTCCTTGATTCTTTCAAGACATAATAATTGAGGGACATGAAGTTAATAAGTATGGCCAAAATATTTGGTATCAAGGGGGAACAAAAAGTCTAAATCATATTTTATCTTCTCAGCTACATGTAAAGGATGAAGATTTAGTTAACTGTAAGACCGAACTTGCTGCCTTGGACCATGAAATTCAGGTTAGGCTTTTAACCTAACCCTTACATTTATTCAGATAATAAACACATATAGTTGTAATCTTCTTTCATGTATGGATATGTAAAGGGTTTGATCAAACTGGCAGAAGAAATAGCACAATCAGGCATTCCAGAAGGTACGAGAAAAATTAATGGGAAATACATTCAATCTCACCTCCTTACAAAATTAGAAGGTATAATGACGATCCTAACCTTTGTTTTCAACAATAGTGGGTTTCTTCCTTTGCACCTGAGTTTTATCGCCTTCTTTCTGCACTTGTATGTGAGATGATGTGTGCTGCCCTCCATGAAAGTTCTCCATTATTACTCAAAAATATATTGTTGACTTATTATTTTTTGGACTCTTCTTTTTCTTTGAAGCTGTACATAAAAAGCTAGAGGATCAAATAAAAGATGTAGATTTGGTGCAATCAAAAGAAGTTCCACTATTCTGGTATGGAATGGCTGAGGTAAGAACAGAAAATTCTATATTTGTTGAAGTATTGATATAATTAAATTTACTATAACCCATCAACTTAAGTTTTAAGATTGATTGGTGATTTAACAATATTTGTTACAATTTTCTCCCTACTCTGAAAGGAAACTAGCTTTCTACATTCTAATATTTGACTTTTACAATTTTCCTAAATGTCAAATGTTTGAATCCTTACCCCCACACTATTTTGAGAACAAAAACAACTAGGTCTGATTTTATTTTATTTTTTAAGTTTCTTTCTTTCATCATTGCCATCATTCTTATAATTTTATATTCATCTTTGAATAGAGTGTGCAAGTTATGGGAACTTTTGATGGCTGGAGTGTAGGGGAGGAGTTGTCACCTGAATACACTGGGGCTTACTCTAAGTTCTCAACAACTTTAAGGCTTAGACCTGGGAGGTATAATTCCTCTCCCTTGCCTTCCTCTCCTCTTGATTTTCTTAATTTACTAATCAAATGATTACCTTTTACGACCAGTATTTATATAAATCGATGATTAGTATAGATATCAACTAATTATAATCATGGCAATCATTGATTAACATTTGATCTATAATGTGTATTAATTTGTGTTATATAAAAAATTGTTTCTTTAGCTGTTGTTTCTGTAATATATATGAAGGTACGAAATCAAGTTCTTAGTGGATGGAGAATGGCAGCTATCACCAGAATTTCCCACTGTTGGCGAAGGTTTGATGGAAAACAATCTGTTAGTTGTGGAATAGTTATGCTGTCAATGATTCAAAAATATGTAAATCAAACTTATAAAGTGTAACTTCAAGTTCATCTTTAACATCTGCTGTTATATAATCATATAATATCCGGTACAGTACTTGGTAGAAGTATGATCTTTACATTTATGGAAGATCTTACACTTGTACCAACTGCCTTGACTTTGCAGCATCTGATTTTTTCAATCCAATTGCCTTGATTGAGTTAGTATTCTCATA

mRNA sequence

ATGAATGAGCCTGCCAATATGGAGATTTGTGCTCGAAGGACTTGTCTGGACAACCAAGTTTTACTGAGTTCCAAGAATGCAAGAAAGTTAAAGATGGAAAACTTGAATAACCCTTCATGTAATATGGCAACCTGGAGCTTTAGGATGGGTTCTTGCAAGTTGATCCATTCTCATGAACCACCTGTAACCACACTTCCTCAAATGTATCGGATTCACAAAAGATATTCCTTGTCTGTCACATCAGAGGAATCTTCAATGCAATCAGAAGATCTTTCTACAGACGAAGAGGAAATTGTGAGTGCAGAGGAACTTCTGGCTCAACCCCTTAGTAGTGAACAGCTGAATGCATTACTGGCTGATTCAGAAAGAGAGAAGCTTGTCAAAAAGCTAAGTCACGCTAATCAGCAGAACAGACTTCTCAAACGACAGCTACATGTAAAGGATGAAGATTTAGTTAACTGTAAGACCGAACTTGCTGCCTTGGACCATGAAATTCAGAGTGTGCAAGTTATGGGAACTTTTGATGGCTGGAGTGTAGGGGAGGAGTTGTCACCTGAATACACTGGGGCTTACTCTAAGTTCTCAACAACTTTAAGGCTTAGACCTGGGAGGTACGAAATCAAGTTCTTAGTGGATGGAGAATGGCAGCTATCACCAGAATTTCCCACTGTTGGCGAAGGTTTGATGGAAAACAATCTGTTAGTTGTGGAATAGTTATGCTGTCAATGATTCAAAAATATGTAAATCAAACTTATAAAGTGTAACTTCAAGTTCATCTTTAACATCTGCTGTTATATAATCATATAATATCCGGTACAGTACTTGGTAGAAGTATGATCTTTACATTTATGGAAGATCTTACACTTGTACCAACTGCCTTGACTTTGCAGCATCTGATTTTTTCAATCCAATTGCCTTGATTGAGTTAGTATTCTCATA

Coding sequence (CDS)

ATGAATGAGCCTGCCAATATGGAGATTTGTGCTCGAAGGACTTGTCTGGACAACCAAGTTTTACTGAGTTCCAAGAATGCAAGAAAGTTAAAGATGGAAAACTTGAATAACCCTTCATGTAATATGGCAACCTGGAGCTTTAGGATGGGTTCTTGCAAGTTGATCCATTCTCATGAACCACCTGTAACCACACTTCCTCAAATGTATCGGATTCACAAAAGATATTCCTTGTCTGTCACATCAGAGGAATCTTCAATGCAATCAGAAGATCTTTCTACAGACGAAGAGGAAATTGTGAGTGCAGAGGAACTTCTGGCTCAACCCCTTAGTAGTGAACAGCTGAATGCATTACTGGCTGATTCAGAAAGAGAGAAGCTTGTCAAAAAGCTAAGTCACGCTAATCAGCAGAACAGACTTCTCAAACGACAGCTACATGTAAAGGATGAAGATTTAGTTAACTGTAAGACCGAACTTGCTGCCTTGGACCATGAAATTCAGAGTGTGCAAGTTATGGGAACTTTTGATGGCTGGAGTGTAGGGGAGGAGTTGTCACCTGAATACACTGGGGCTTACTCTAAGTTCTCAACAACTTTAAGGCTTAGACCTGGGAGGTACGAAATCAAGTTCTTAGTGGATGGAGAATGGCAGCTATCACCAGAATTTCCCACTGTTGGCGAAGGTTTGATGGAAAACAATCTGTTAGTTGTGGAATAG

Protein sequence

MNEPANMEICARRTCLDNQVLLSSKNARKLKMENLNNPSCNMATWSFRMGSCKLIHSHEPPVTTLPQMYRIHKRYSLSVTSEESSMQSEDLSTDEEEIVSAEELLAQPLSSEQLNALLADSEREKLVKKLSHANQQNRLLKRQLHVKDEDLVNCKTELAALDHEIQSVQVMGTFDGWSVGEELSPEYTGAYSKFSTTLRLRPGRYEIKFLVDGEWQLSPEFPTVGEGLMENNLLVVE

Homology

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BLAST of Lsi05G003300 vs. Swiss-Prot
Match: PTST_ARATH (Protein PTST, chloroplastic OS=Arabidopsis thaliana GN=PTST PE=1 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 9.7e-28
Identity = 81/175 (46.29%), Postives = 107/175 (61.14%), Query Frame = 1


Query: 77  LSVTSEESSMQSEDLSTDEEEIVSAEELLAQPLSSEQLNALLADSEREKLV------KKL 136
           LS  ++++      L T E EI + +  LA  L   ++ AL+  +E    +      +K+
Sbjct: 105 LSEANQQNRFLKRQLKTQEHEITNIKTELA--LMELEVQALVKLAEEIANLGIPQGSRKI 164

Query: 137 SHANQQNRLLKRQLHVKDEDLVNCKTELAALDHEI--------QSVQVMGTFDGWSVGEE 196
           S    Q+ LL R   V+ +     K   AA   E+        +SVQVMG+FDGWS  E+
Sbjct: 165 SGKYIQSHLLSRLDAVQKKMKEQIKGVEAAQSKEVHVFWIGMAESVQVMGSFDGWSQRED 224

Query: 197 LSPEYTGAYSKFSTTLRLRPGRYEIKFLVDGEWQLSPEFPTVGEGLMENNLLVVE 238
           LSPEY+  ++KFSTTL LRPGRYE+KFLVDGEWQ+SPEFPT GEGLMENN+LVVE
Sbjct: 225 LSPEYSALFTKFSTTLFLRPGRYEMKFLVDGEWQISPEFPTSGEGLMENNVLVVE 277

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BLAST of Lsi05G003300 vs. TrEMBL
Match: W9SBS9_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_027204 PE=4 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 1.6e-48
Identity = 110/167 (65.87%), Postives = 130/167 (77.84%), Query Frame = 1


Query: 73  KRYSLSVTSEESSMQSEDLSTDEEEIV--SAEELLAQPLSSEQLNALLADSEREKLVKKL 132
           + +  SV  EESS    + + + EE    S EELL++PLS+         SER  L++KL
Sbjct: 31  RAHCTSVNLEESSSTGSEDNPNAEESSQESPEELLSKPLSN---------SERANLLRKL 90

Query: 133 SHANQQNRLLKRQLHVKDEDLVNCKTELAALDHEIQSVQVMGTFDGWSVGEELSPEYTGA 192
           S ANQQNR LKRQLH+K+E LV+ K+ELA ++ EIQSVQVMGTFDGWS GE LSPEYTG+
Sbjct: 91  SEANQQNRFLKRQLHMKEEALVDFKSELAVMELEIQSVQVMGTFDGWSQGESLSPEYTGS 150

Query: 193 YSKFSTTLRLRPGRYEIKFLVDGEWQLSPEFPTVGEGLMENNLLVVE 238
           Y+KFSTTL LRPGRYEIKFLVDGEWQLSPEFPTVGEGLM+NNLL+VE
Sbjct: 151 YTKFSTTLPLRPGRYEIKFLVDGEWQLSPEFPTVGEGLMQNNLLIVE 188

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BLAST of Lsi05G003300 vs. TrEMBL
Match: A0A0A0L7M7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G316260 PE=4 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 2.0e-43
Identity = 97/112 (86.61%), Postives = 100/112 (89.29%), Query Frame = 1


Query: 4   PANMEICARRTCLDNQVLLSSKNARKLKMENLNNPSCNMATWSFRMGSCKLIHSHEPPVT 63
           PANMEICA RTCLDNQVLLSSKNA KLK E LNNPSCN+ATWSFR GSCKLIHSHEP V 
Sbjct: 134 PANMEICAPRTCLDNQVLLSSKNATKLKREKLNNPSCNIATWSFRTGSCKLIHSHEPSVI 193

Query: 64  TLPQMYRIHKRYSLSVTSEESSMQSEDLSTDEEEIVSAEELLAQPLSSEQLN 116
           T PQ Y+I KRYSL VTSEESS QSEDLSTDEEEIVSAEELLAQPLSSEQ+N
Sbjct: 194 THPQTYQILKRYSLPVTSEESSTQSEDLSTDEEEIVSAEELLAQPLSSEQVN 245

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BLAST of Lsi05G003300 vs. TrEMBL
Match: A0A0A0LAW5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G314760 PE=4 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 1.0e-31
Identity = 91/173 (52.60%), Postives = 114/173 (65.90%), Query Frame = 1


Query: 77  LSVTSEESSMQSEDLSTDEEEIVSAE-ELLAQPLSSEQLNAL---LADSEREKLVKKLSH 136
           LS  ++++ +    L   +E++V+ + EL A     + L  L   +A S   +  +K++ 
Sbjct: 82  LSHANQQNRLLKRQLHVKDEDLVNCKTELAALDHDIQGLIKLAEEIAQSGIPERTRKING 141

Query: 137 ANQQNRLLKR--QLH------VKDEDLVNCKTELAALDHEIQSVQVMGTFDGWSVGEELS 196
              Q+ LL +   +H      +KD DLV  K          ++VQVMGTFDGWSVGE+LS
Sbjct: 142 KYIQSHLLTKLEAVHKKIMDQIKDVDLVQSKEVPLFWYGMAENVQVMGTFDGWSVGEDLS 201

Query: 197 PEYTGAYSKFSTTLRLRPGRYEIKFLVDGEWQLSPEFPTVGEGLMENNLLVVE 238
           PEYTGAYSKFSTTLRLRPGRYEIKFLVDGEWQLS EFPTVGEGLMENNLLVVE
Sbjct: 202 PEYTGAYSKFSTTLRLRPGRYEIKFLVDGEWQLSTEFPTVGEGLMENNLLVVE 254

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BLAST of Lsi05G003300 vs. TrEMBL
Match: F6HSK3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0006g01240 PE=4 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 4.7e-29
Identity = 85/173 (49.13%), Postives = 109/173 (63.01%), Query Frame = 1


Query: 77  LSVTSEESSMQSEDLSTDEEEIVSAE-ELLAQPLSSEQLNAL---LADSEREKLVKKLSH 136
           LS  ++++ +    L   E+ +V+ + EL    L  + L +L   +A S   K  +K++ 
Sbjct: 135 LSEANQQNRILKRQLYIKEDALVNFKSELAVMELEVQALVSLAEEIAKSGIPKGSRKING 194

Query: 137 ANQQNRLLKR--------QLHVKDEDLVNCKTELAALDHEIQSVQVMGTFDGWSVGEELS 196
              Q+ L  R        +  +KD D V  K          +SVQVMGTFDGWS GE LS
Sbjct: 195 KYIQSHLHSRLEAVHEKLKEQIKDVDAVQSKEVSIIWCGMAESVQVMGTFDGWSQGEHLS 254

Query: 197 PEYTGAYSKFSTTLRLRPGRYEIKFLVDGEWQLSPEFPTVGEGLMENNLLVVE 238
           PEYTG+++KFSTTL LRPGRYEIKFLVDGEWQLSPEFPTVGEGLMENNLL+V+
Sbjct: 255 PEYTGSFTKFSTTLMLRPGRYEIKFLVDGEWQLSPEFPTVGEGLMENNLLIVK 307

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BLAST of Lsi05G003300 vs. TrEMBL
Match: V4UVX6_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10012334mg PE=4 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 6.1e-29
Identity = 87/193 (45.08%), Postives = 116/193 (60.10%), Query Frame = 1


Query: 57  SHEPPVTTLPQMYRIHKRYSLSVTSEESSMQSEDLSTDEEEIVSAE-ELLAQPLSSEQLN 116
           S++  ++ L    R      LS  ++++      L   E+ +V  + EL    L  + L 
Sbjct: 104 SNDELMSLLADSERKKLMIKLSEANQQNRFLKRQLLIKEDALVDFKSELAVMELEIQALV 163

Query: 117 AL---LADSEREKLVKKLSHANQQNRLLKRQLHVKDEDLVNCKTELAALDHEI------- 176
           +L   +A S   +  +K++    Q+ LL R   V ++     K  +AA   E+       
Sbjct: 164 SLAEEIAQSGIPEDSRKINGKFIQSHLLSRLEAVHEKLKEQIKDVIAAQSKEVPLFWRGM 223

Query: 177 -QSVQVMGTFDGWSVGEELSPEYTGAYSKFSTTLRLRPGRYEIKFLVDGEWQLSPEFPTV 236
            QSVQVMGTFDGWS GE LSPEY G+++KFSTTL LRPGRYEIKFL+DGEWQLSPEFPTV
Sbjct: 224 AQSVQVMGTFDGWSQGEHLSPEYNGSFTKFSTTLMLRPGRYEIKFLIDGEWQLSPEFPTV 283

Query: 237 GEGLMENNLLVVE 238
           GEGLM+NNLL+VE
Sbjct: 284 GEGLMKNNLLIVE 296

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BLAST of Lsi05G003300 vs. TAIR10
Match: AT5G39790.1 (AT5G39790.1 5'-AMP-activated protein kinase-related)

HSP 1 Score: 123.6 bits (309), Expect = 1.6e-28
Identity = 82/179 (45.81%), Postives = 108/179 (60.34%), Query Frame = 1


Query: 73  KRYSLSVTSEESSMQSEDLSTDEEEIVSAEELLAQPLSSEQLNALLADSEREKLV----- 132
           +R  L     E++ Q+  L   E EI + +  LA  L   ++ AL+  +E    +     
Sbjct: 97  ERSKLVKKLSEANQQNRFLKRQEHEITNIKTELA--LMELEVQALVKLAEEIANLGIPQG 156

Query: 133 -KKLSHANQQNRLLKRQLHVKDEDLVNCKTELAALDHEI--------QSVQVMGTFDGWS 192
            +K+S    Q+ LL R   V+ +     K   AA   E+        +SVQVMG+FDGWS
Sbjct: 157 SRKISGKYIQSHLLSRLDAVQKKMKEQIKGVEAAQSKEVHVFWIGMAESVQVMGSFDGWS 216

Query: 193 VGEELSPEYTGAYSKFSTTLRLRPGRYEIKFLVDGEWQLSPEFPTVGEGLMENNLLVVE 238
             E+LSPEY+  ++KFSTTL LRPGRYE+KFLVDGEWQ+SPEFPT GEGLMENN+LVVE
Sbjct: 217 QREDLSPEYSALFTKFSTTLFLRPGRYEMKFLVDGEWQISPEFPTSGEGLMENNVLVVE 273

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BLAST of Lsi05G003300 vs. TAIR10
Match: AT1G27070.1 (AT1G27070.1 5'-AMP-activated protein kinase-related)

HSP 1 Score: 68.2 bits (165), Expect = 7.9e-12
Identity = 52/170 (30.59%), Postives = 84/170 (49.41%), Query Frame = 1


Query: 78  SVTSEESSMQSEDLSTD----EEEIVSAEELLAQPLSS-----EQLNALLADSER--EKL 137
           S  SE ++   E+LS D    E EI+ A+  L    +       ++   + D++R   + 
Sbjct: 379 SKDSETTAGNLENLSDDWEYKENEIIHAQNKLRSTRAKLAVLEGKMAMAIIDAQRIVREK 438

Query: 138 VKKLSHANQQNRLLKRQLHVKDEDLVNCKTELAALDHEIQSVQVMGTFDGWSVGEELSPE 197
            +++ HA++  RLL+              T      +    V + G+FDGWS   ++   
Sbjct: 439 QRRIDHASRALRLLR--------------TASIVWPNSASEVLLTGSFDGWSTQRKMKKA 498

Query: 198 YTGAYSKFSTTLRLRPGRYEIKFLVDGEWQLSPEFPTVGEGLMENNLLVV 237
             G    FS +L+L PG+YEIKF+VDG+W++ P  P V  G  ENNLL++
Sbjct: 499 ENGV---FSLSLKLYPGKYEIKFIVDGQWKVDPLRPIVTSGGYENNLLII 531

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BLAST of Lsi05G003300 vs. TAIR10
Match: AT5G03420.1 (AT5G03420.1 5'-AMP-activated protein kinase-related)

HSP 1 Score: 67.0 bits (162), Expect = 1.8e-11
Identity = 45/137 (32.85%), Postives = 71/137 (51.82%), Query Frame = 1


Query: 113 QLNALLADSEREKLVKKLSHANQQNRLLKRQLHV--KDEDLVNCKTELAALDHEI----- 172
           +L+ L    E+EKL   +     +  + K Q+ +  K+ +L   +  L+ L   +     
Sbjct: 460 ELSRLKEQIEKEKLSLSVLQRQAETEIQKAQMLISEKEVELQEAEESLSGLQEVVIEYCG 519

Query: 173 --QSVQVMGTFDGWS--VGEELSPEYT-GAYSKFSTTLRLRPGRYEIKFLVDGEWQLSPE 232
              +V+V G+F+GW   VG EL    + G    +ST L L PG YEIKF+VDG+W   P+
Sbjct: 520 DGNAVEVTGSFNGWQHRVGMELQASKSIGKQKCWSTLLWLYPGTYEIKFIVDGQWITDPQ 579

Query: 233 FPTVGEGLMENNLLVVE 238
             +V  G + NN+L V+
Sbjct: 580 KDSVTRGHISNNILKVD 596

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BLAST of Lsi05G003300 vs. NCBI nr
Match: gi|659114426|ref|XP_008457048.1| (PREDICTED: uncharacterized protein LOC103496819 isoform X1 [Cucumis melo])

HSP 1 Score: 275.4 bits (703), Expect = 9.3e-71
Identity = 157/226 (69.47%), Postives = 170/226 (75.22%), Query Frame = 1


Query: 7   MEICARRTCLDNQVLLSSKNARKLKMENLNNPSCNMATWSFRMGSCKLIHSHEPPVTTLP 66
           MEICA RTCLDN VLLSSK ARKLK ENLNNPSCNMATWSFR GSCKLIHSHEP VT  P
Sbjct: 1   MEICAPRTCLDNLVLLSSKKARKLKRENLNNPSCNMATWSFRTGSCKLIHSHEPSVTIRP 60

Query: 67  QMYRIHKRYSLSVTSEESSMQSEDLSTDEEEIVSAEELLAQPLSSEQLNALLADSEREKL 126
           Q Y+I KRYSL VTSEESSMQSEDLST EEEIVS EELLAQPLSSEQLNALLADSER++L
Sbjct: 61  QSYQILKRYSLPVTSEESSMQSEDLSTHEEEIVSGEELLAQPLSSEQLNALLADSERDRL 120

Query: 127 VKKLSHANQQNRLLKRQLHVKDEDLVNCKTELAALDHEIQSVQVMGTFDGWSVGEELSPE 186
           VKKLSHANQQNRLLKRQLHVKDEDLVNCKTELAALDHEIQ +  +      S   E + +
Sbjct: 121 VKKLSHANQQNRLLKRQLHVKDEDLVNCKTELAALDHEIQGLIKLAEEIAQSGIPERTRK 180

Query: 187 YTGAYSKFSTTLRLRPGRYEI-KFLVDGEWQLSPEFPTVGEGLMEN 232
             G Y +     +L     +I   + D +   S E P    G+ EN
Sbjct: 181 INGKYIQSHLLTKLEAVHKKIVDQIKDVDLVQSKEVPLFWYGMAEN 226

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BLAST of Lsi05G003300 vs. NCBI nr
Match: gi|449456196|ref|XP_004145836.1| (PREDICTED: uncharacterized protein LOC101218713 [Cucumis sativus])

HSP 1 Score: 275.0 bits (702), Expect = 1.2e-70
Identity = 156/226 (69.03%), Postives = 171/226 (75.66%), Query Frame = 1


Query: 7   MEICARRTCLDNQVLLSSKNARKLKMENLNNPSCNMATWSFRMGSCKLIHSHEPPVTTLP 66
           MEICA RTCLDNQVLLSSKNA KLK E LNNPSCN+ATWSFR GSCKLIHSHEP V T P
Sbjct: 1   MEICAPRTCLDNQVLLSSKNATKLKREKLNNPSCNIATWSFRTGSCKLIHSHEPSVITHP 60

Query: 67  QMYRIHKRYSLSVTSEESSMQSEDLSTDEEEIVSAEELLAQPLSSEQLNALLADSEREKL 126
           Q Y+I KRYSL VTSEESS QSEDLSTDEEEIVSAEELLAQPLSSEQLNALLADSER++L
Sbjct: 61  QTYQILKRYSLPVTSEESSTQSEDLSTDEEEIVSAEELLAQPLSSEQLNALLADSERDRL 120

Query: 127 VKKLSHANQQNRLLKRQLHVKDEDLVNCKTELAALDHEIQSVQVMGTFDGWSVGEELSPE 186
           VKKLSHANQQNRLLKRQLHVKDEDLVNCKTELAALDH+IQ +  +      S   E + +
Sbjct: 121 VKKLSHANQQNRLLKRQLHVKDEDLVNCKTELAALDHDIQGLIKLAEEIAQSGIPERTRK 180

Query: 187 YTGAYSKFSTTLRLRPGRYEIKFLV-DGEWQLSPEFPTVGEGLMEN 232
             G Y +     +L     +I   + D +   S E P    G+ EN
Sbjct: 181 INGKYIQSHLLTKLEAVHKKIMDQIKDVDLVQSKEVPLFWYGMAEN 226

[Close]

BLAST of Lsi05G003300 vs. NCBI nr
Match: gi|659114428|ref|XP_008457049.1| (PREDICTED: uncharacterized protein LOC103496819 isoform X2 [Cucumis melo])

HSP 1 Score: 217.2 bits (552), Expect = 3.0e-53
Identity = 135/226 (59.73%), Postives = 148/226 (65.49%), Query Frame = 1


Query: 7   MEICARRTCLDNQVLLSSKNARKLKMENLNNPSCNMATWSFRMGSCKLIHSHEPPVTTLP 66
           MEICA RTCLDN VLLSSK ARKLK ENLNNPSCNMATWSFR                  
Sbjct: 1   MEICAPRTCLDNLVLLSSKKARKLKRENLNNPSCNMATWSFR------------------ 60

Query: 67  QMYRIHKRYSLSVTSEESSMQSEDLSTDEEEIVSAEELLAQPLSSEQLNALLADSEREKL 126
                      +VTSEESSMQSEDLST EEEIVS EELLAQPLSSEQLNALLADSER++L
Sbjct: 61  -----------TVTSEESSMQSEDLSTHEEEIVSGEELLAQPLSSEQLNALLADSERDRL 120

Query: 127 VKKLSHANQQNRLLKRQLHVKDEDLVNCKTELAALDHEIQSVQVMGTFDGWSVGEELSPE 186
           VKKLSHANQQNRLLKRQLHVKDEDLVNCKTELAALDHEIQ +  +      S   E + +
Sbjct: 121 VKKLSHANQQNRLLKRQLHVKDEDLVNCKTELAALDHEIQGLIKLAEEIAQSGIPERTRK 180

Query: 187 YTGAYSKFSTTLRLRPGRYEI-KFLVDGEWQLSPEFPTVGEGLMEN 232
             G Y +     +L     +I   + D +   S E P    G+ EN
Sbjct: 181 INGKYIQSHLLTKLEAVHKKIVDQIKDVDLVQSKEVPLFWYGMAEN 197

[Close]

BLAST of Lsi05G003300 vs. NCBI nr
Match: gi|703147260|ref|XP_010109007.1| (hypothetical protein L484_027204 [Morus notabilis])

HSP 1 Score: 201.1 bits (510), Expect = 2.2e-48
Identity = 110/167 (65.87%), Postives = 130/167 (77.84%), Query Frame = 1


Query: 73  KRYSLSVTSEESSMQSEDLSTDEEEIV--SAEELLAQPLSSEQLNALLADSEREKLVKKL 132
           + +  SV  EESS    + + + EE    S EELL++PLS+         SER  L++KL
Sbjct: 31  RAHCTSVNLEESSSTGSEDNPNAEESSQESPEELLSKPLSN---------SERANLLRKL 90

Query: 133 SHANQQNRLLKRQLHVKDEDLVNCKTELAALDHEIQSVQVMGTFDGWSVGEELSPEYTGA 192
           S ANQQNR LKRQLH+K+E LV+ K+ELA ++ EIQSVQVMGTFDGWS GE LSPEYTG+
Sbjct: 91  SEANQQNRFLKRQLHMKEEALVDFKSELAVMELEIQSVQVMGTFDGWSQGESLSPEYTGS 150

Query: 193 YSKFSTTLRLRPGRYEIKFLVDGEWQLSPEFPTVGEGLMENNLLVVE 238
           Y+KFSTTL LRPGRYEIKFLVDGEWQLSPEFPTVGEGLM+NNLL+VE
Sbjct: 151 YTKFSTTLPLRPGRYEIKFLVDGEWQLSPEFPTVGEGLMQNNLLIVE 188

[Close]

BLAST of Lsi05G003300 vs. NCBI nr
Match: gi|700202680|gb|KGN57813.1| (hypothetical protein Csa_3G316260 [Cucumis sativus])

HSP 1 Score: 184.1 bits (466), Expect = 2.8e-43
Identity = 97/112 (86.61%), Postives = 100/112 (89.29%), Query Frame = 1


Query: 4   PANMEICARRTCLDNQVLLSSKNARKLKMENLNNPSCNMATWSFRMGSCKLIHSHEPPVT 63
           PANMEICA RTCLDNQVLLSSKNA KLK E LNNPSCN+ATWSFR GSCKLIHSHEP V 
Sbjct: 134 PANMEICAPRTCLDNQVLLSSKNATKLKREKLNNPSCNIATWSFRTGSCKLIHSHEPSVI 193

Query: 64  TLPQMYRIHKRYSLSVTSEESSMQSEDLSTDEEEIVSAEELLAQPLSSEQLN 116
           T PQ Y+I KRYSL VTSEESS QSEDLSTDEEEIVSAEELLAQPLSSEQ+N
Sbjct: 194 THPQTYQILKRYSLPVTSEESSTQSEDLSTDEEEIVSAEELLAQPLSSEQVN 245

The following BLAST results are available for this feature:

BLAST of Lsi05G003300 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PTST_ARATH	9.7e-28	46.29	Protein PTST, chloroplastic OS=Arabidopsis thaliana GN=PTST PE=1 SV=1	[more]

BLAST of Lsi05G003300 vs. TrEMBL


Match Name	E-value	Identity	Description
W9SBS9_9ROSA	1.6e-48	65.87	Uncharacterized protein OS=Morus notabilis GN=L484_027204 PE=4 SV=1	[more]
A0A0A0L7M7_CUCSA	2.0e-43	86.61	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G316260 PE=4 SV=1	[more]
A0A0A0LAW5_CUCSA	1.0e-31	52.60	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G314760 PE=4 SV=1	[more]
F6HSK3_VITVI	4.7e-29	49.13	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0006g01240 PE=4 SV=...	[more]
V4UVX6_9ROSI	6.1e-29	45.08	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10012334mg PE=4 SV=1	[more]

BLAST of Lsi05G003300 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G39790.1	1.6e-28	45.81	5'-AMP-activated protein kinase-related	[more]
AT1G27070.1	7.9e-12	30.59	5'-AMP-activated protein kinase-related	[more]
AT5G03420.1	1.8e-11	32.85	5'-AMP-activated protein kinase-related	[more]

BLAST of Lsi05G003300 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659114426\|ref\|XP_008457048.1\|	9.3e-71	69.47	PREDICTED: uncharacterized protein LOC103496819 isoform X1 [Cucumis melo]	[more]
gi\|449456196\|ref\|XP_004145836.1\|	1.2e-70	69.03	PREDICTED: uncharacterized protein LOC101218713 [Cucumis sativus]	[more]
gi\|659114428\|ref\|XP_008457049.1\|	3.0e-53	59.73	PREDICTED: uncharacterized protein LOC103496819 isoform X2 [Cucumis melo]	[more]
gi\|703147260\|ref\|XP_010109007.1\|	2.2e-48	65.87	hypothetical protein L484_027204 [Morus notabilis]	[more]
gi\|700202680\|gb\|KGN57813.1\|	2.8e-43	86.61	hypothetical protein Csa_3G316260 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR014756	Ig_E-set

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
biological_process	GO:0016310	phosphorylation
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0017070	U6 snRNA binding
molecular_function	GO:0016301	kinase activity
molecular_function	GO:0030247	polysaccharide binding
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G003300.1	Lsi05G003300.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014756	Immunoglobulin E-set	unknown	SSF81296	E set domains	coord: 161..236 score: 2.75
None	No IPR available	unknown	Coil	Coil	coord: 123..143 scor
None	No IPR available	PANTHER	PTHR10343	5'-AMP-ACTIVATED PROTEIN KINASE , BETA SUBUNIT	coord: 13..237 score: 5.5
None	No IPR available	PANTHER	PTHR10343:SF61	STARCH BINDING SCAFFOLD PROTEIN-RELATED	coord: 13..237 score: 5.5

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi05G003300	Csa3G314760	Cucumber (Chinese Long) v2	culsiB212
Lsi05G003300	CSPI03G21460	Wild cucumber (PI 183967)	cpilsiB210
Lsi05G003300	Cucsa.375380	Cucumber (Gy14) v1	cgylsiB607
Lsi05G003300	CmaCh01G003640	Cucurbita maxima (Rimu)	cmalsiB440
Lsi05G003300	CmoCh01G003810	Cucurbita moschata (Rifu)	cmolsiB430
Lsi05G003300	Cla013193	Watermelon (97103) v1	lsiwmB361
Lsi05G003300	MELO3C020256	Melon (DHL92) v3.5.1	lsimeB368
Lsi05G003300	ClCG05G011250	Watermelon (Charleston Gray)	lsiwcgB332
Lsi05G003300	MELO3C020256.2	Melon (DHL92) v3.6.1	lsimedB384
Lsi05G003300	Cla97C05G093410	Watermelon (97103) v2	lsiwmbB329
Lsi05G003300	Bhi01G001511	Wax gourd	lsiwgoB447
Lsi05G003300	Cp4.1LG02g16930	Cucurbita pepo (Zucchini)	cpelsiB470
Lsi05G003300	CsGy3G020740	Cucumber (Gy14) v2	cgyblsiB199
Lsi05G003300	Carg07451	Silver-seed gourd	carlsiB700
Lsi05G003300	CsaV3_3G021180	Cucumber (Chinese Long) v3	cuclsiB231

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi05G003300	Cucurbita maxima (Rimu)	cmalsiB249
Lsi05G003300	Cucurbita moschata (Rifu)	cmolsiB237
Lsi05G003300	Cucurbita pepo (Zucchini)	cpelsiB511
Lsi05G003300	Cucumber (Gy14) v2	cgyblsiB192
Lsi05G003300	Silver-seed gourd	carlsiB464