Lsi05G003220 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi05G003220
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	MLP-like protein 31
Location	chr05 : 4097872 .. 4099148 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTGAAGCTAATTTAGAAGCTTCAAAATGGCTCAGATTGCTAAGATCTCAGAGCAAGTGAAGCTCAAGTCTTCTGCTCACAAATTCTATGAGTTTTTCAAGAATAATATGGACTATGTCTTTGTTCAAATGTTCCCTGAAATTTACAAGAGCTGTAAGGTTGTCGAAGGAAATGGTTTTTCTCATGGCAGCGTCATCCATTTAAAATATAACGCAGGTGAGGATCTGTATCAACCATTCTTTCGAACTTTTTCGGTAATGGTTCCTACTACTTTCAAATGTTCTATTTTAATTTTGAACTTTCAAACGGATCCCTTTTTTCTCCAAAAAAAAAAAAAAACGTATTTATTTATTTTTTATTTAAAAACTTATTCCAAAAACACTATTATTAAGAATCAATAACAAAAAGATCATTAGGGTTTGCTAGACAACCTCCACAACGTAACATCATATTCTTGCTTATTGATATTAATATATCATGTGTCTTAACTAATTGAAGTATGCTTAATTTAACTGATATGAATACAATTGACATGTCGGGTTTTATATGTCAATTATATATAGGGATAGTTGCAAATGTAGCAATCAGATTCAAAGTAATAGCATATATAGCAACATTTTTAAAAAATTGCAAATATAGCAAAGTTTATCACTGATAGACTTTTATGGTTTAGGTGGTCTATCAATGATAGAAGTTTATCACTGATAGACTTTGCTATATTTGCAATTTTTTTAAAAAATGTTCTATACACTTGATTATTATTCCTGAAATTGCTACTCATTACAACTACCTTTATATATACACCTTTATAACTGAGTTTCAATCCAAGTCCTTAGGATTTCTTTTGTTTTCTGGTGGAAATTTACATAGGAAGGCCAGAAGAGGTAAAAGAGAGGCTGGCTATTGATGATGCAAACAAATCAATAACTTTTGAGTGCATTGAAGGAGATCCATTAAGAGATTTTGAAGTGATCAAATTGAAATTTGAAGTTTTTGAGAATGGTAGCAATGGCAGCTCAGTTAATTGGTCATTAGAATTTGTGAAGGCAAATGAAGATGTGGCTGCACCACATCACTATCTCCTATGTGTAGCTAAAGTTGGCAAAGGCCTTGATGATTTCCTTTGCAACAACTGAACTAAAACCCATCCAAATTAGCTTGGATATTGAAATATGATCTAAATAAATTATCTCCTTATCTTTATTATTGTTGTTATCATATGTGTATTTTGAAGCTTAATTGAAAGAAATATTAATGGTGATGGTGCCATATTCTA

mRNA sequence

ATTTGAAGCTAATTTAGAAGCTTCAAAATGGCTCAGATTGCTAAGATCTCAGAGCAAGTGAAGCTCAAGTCTTCTGCTCACAAATTCTATGAGTTTTTCAAGAATAATATGGACTATGTCTTTGTTCAAATGTTCCCTGAAATTTACAAGAGCTGTAAGGTTGTCGAAGGAAATGGTTTTTCTCATGGCAGCGTCATCCATTTAAAATATAACGCAGGAAGGCCAGAAGAGGTAAAAGAGAGGCTGGCTATTGATGATGCAAACAAATCAATAACTTTTGAGTGCATTGAAGGAGATCCATTAAGAGATTTTGAAGTGATCAAATTGAAATTTGAAGTTTTTGAGAATGGTAGCAATGGCAGCTCAGTTAATTGGTCATTAGAATTTGTGAAGGCAAATGAAGATGTGGCTGCACCACATCACTATCTCCTATGTGTAGCTAAAGTTGGCAAAGGCCTTGATGATTTCCTTTGCAACAACTGAACTAAAACCCATCCAAATTAGCTTGGATATTGAAATATGATCTAAATAAATTATCTCCTTATCTTTATTATTGTTGTTATCATATGTGTATTTTGAAGCTTAATTGAAAGAAATATTAATGGTGATGGTGCCATATTCTA

Coding sequence (CDS)

ATGGCTCAGATTGCTAAGATCTCAGAGCAAGTGAAGCTCAAGTCTTCTGCTCACAAATTCTATGAGTTTTTCAAGAATAATATGGACTATGTCTTTGTTCAAATGTTCCCTGAAATTTACAAGAGCTGTAAGGTTGTCGAAGGAAATGGTTTTTCTCATGGCAGCGTCATCCATTTAAAATATAACGCAGGAAGGCCAGAAGAGGTAAAAGAGAGGCTGGCTATTGATGATGCAAACAAATCAATAACTTTTGAGTGCATTGAAGGAGATCCATTAAGAGATTTTGAAGTGATCAAATTGAAATTTGAAGTTTTTGAGAATGGTAGCAATGGCAGCTCAGTTAATTGGTCATTAGAATTTGTGAAGGCAAATGAAGATGTGGCTGCACCACATCACTATCTCCTATGTGTAGCTAAAGTTGGCAAAGGCCTTGATGATTTCCTTTGCAACAACTGA

Protein sequence

MAQIAKISEQVKLKSSAHKFYEFFKNNMDYVFVQMFPEIYKSCKVVEGNGFSHGSVIHLKYNAGRPEEVKERLAIDDANKSITFECIEGDPLRDFEVIKLKFEVFENGSNGSSVNWSLEFVKANEDVAAPHHYLLCVAKVGKGLDDFLCNN

Homology

[Close]

BLAST of Lsi05G003220 vs. Swiss-Prot
Match: MLP34_ARATH (MLP-like protein 34 OS=Arabidopsis thaliana GN=MLP34 PE=2 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 5.1e-14
Identity = 47/145 (32.41%), Postives = 81/145 (55.86%), Query Frame = 1


Query: 7   ISEQVKLKSSAHKFYEFFKNNMDYVFVQMFPEIYKSCKVVEGNGFSHGSVIHLKY-NAGR 66
           +  +V++K+SA KF+  F     +V  +  P   +SC + EG+  + GS++   Y + G 
Sbjct: 169 LETEVEIKASAEKFHHMFAGKPHHVS-KATPGNIQSCDLHEGDWGTVGSIVFWNYVHDGE 228

Query: 67  PEEVKERL-AIDDANKSITFECIEGDPLRDFEVIKLKFEVF-ENGSNGSSVNWSLEFVKA 126
            +  KER+ A+D     ITF  IEGD +++++   +  +V  ++G +GS V+W  E+ K 
Sbjct: 229 AKVAKERIEAVDPEKNLITFRVIEGDLMKEYKSFVITIQVTPKHGGSGSVVHWHFEYEKI 288

Query: 127 NEDVAAPHHYLLCVAKVGKGLDDFL 149
           NE+VA P   L    +V K +D+ L
Sbjct: 289 NEEVAHPETLLQFAVEVSKEIDEHL 312

[Close]

BLAST of Lsi05G003220 vs. Swiss-Prot
Match: ML146_PAPSO (Major latex protein 146 OS=Papaver somniferum GN=MLP146 PE=2 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 3.3e-13
Identity = 43/148 (29.05%), Postives = 75/148 (50.68%), Query Frame = 1


Query: 4   IAKISEQVKLKSSAHKFYEFFKNNMDYVFVQMFPEIYKSCKVVEGNGFSHGSVIHLKYNA 63
           + K+  + ++  +A K+Y+ +K++ D   V   P IY S K VEG+G + G V    Y  
Sbjct: 11  VGKLVMESEVNCNADKYYKLYKHHEDLPSV--IPHIYTSVKAVEGHGTTSGCVKEWGYIL 70

Query: 64  -GRPEEVKERLAIDDANKSITFECIEGDPLRDFEVIKLKFEVF-ENGSNGSSVNWSLEFV 123
            G+P   KE+   +D  ++I    + GD + D++       V  ++  +G  V W++++ 
Sbjct: 71  EGKPLSCKEKTTYNDETRTIHHMVVAGDLMNDYKKFDATLVVNPKSNGHGCIVKWTIDYE 130

Query: 124 KANEDVAAPHHYLLCVAKVGKGLDDFLC 150
           K NED   P  YL C  ++ + L   LC
Sbjct: 131 KMNEDSPVPFGYLACYQQITEDLSSHLC 156

[Close]

BLAST of Lsi05G003220 vs. Swiss-Prot
Match: MLP15_PAPSO (Major latex protein 15 OS=Papaver somniferum GN=MLP15 PE=2 SV=2)

HSP 1 Score: 73.9 bits (180), Expect = 1.6e-12
Identity = 48/149 (32.21%), Postives = 75/149 (50.34%), Query Frame = 1


Query: 4   IAKISEQVKLKSSAHKFYEFFKNNMDYVFVQMFPEIYKSCKVVEGNGFSHGSVIHLKYNA 63
           + K+  + ++  +A K+Y+ FK++ D       P IY S K VEG+G + G V    Y  
Sbjct: 11  VGKLITESEVNCNADKYYQIFKHHED--LPSAIPHIYTSVKAVEGHGTTSGCVKEWCYIL 70

Query: 64  -GRPEEVKERLAIDDANKSITFECIEGDPLRDFE--VIKLKFEVFENGSNGSSVNWSLEF 123
            G+P  VKE+   +D  ++I    IEG  + D++  V  L  +   NG  GS V W +++
Sbjct: 71  EGKPLTVKEKTTYNDETRTINHNGIEGGMMNDYKKFVATLVVKPKANG-QGSIVTWIVDY 130

Query: 124 VKANEDVAAPHHYLLCVAKVGKGLDDFLC 150
            K NED   P  YL    +  + L+  LC
Sbjct: 131 EKINEDSPVPFDYLAFFQQNIEDLNSHLC 156

[Close]

BLAST of Lsi05G003220 vs. Swiss-Prot
Match: MLP31_ARATH (MLP-like protein 31 OS=Arabidopsis thaliana GN=MLP31 PE=2 SV=2)

HSP 1 Score: 73.6 bits (179), Expect = 2.1e-12
Identity = 45/148 (30.41%), Postives = 80/148 (54.05%), Query Frame = 1


Query: 6   KISEQVKLKSSAHKFYEFFKNNMDYVFVQMFPEIYKSCKVVEGNGFSHGSVIHLKY-NAG 65
           K+   +++K+SA KF+  F     +V  +  P   + C++ EG+    GS++   Y + G
Sbjct: 23  KLETDIEIKASAGKFHHMFAGRPHHVS-KATPGKIQGCELHEGDWGKVGSIVFWNYVHDG 82

Query: 66  RPEEVKERL-AIDDANKSITFECIEGDPLRDFEVIKLKFEVF-ENGSNGSSVNWSLEFVK 125
             +  KER+ A++     ITF  IEGD L++++   +  +V  + G  GS V+W +E+ K
Sbjct: 83  EAKVAKERIEAVEPEKNLITFRVIEGDLLKEYKSFVITIQVTPKRGGPGSVVHWHVEYEK 142

Query: 126 ANEDVAAPHHYLLCVAKVGKGLDDFLCN 151
            ++ VA P  +L    +V K +D+ L N
Sbjct: 143 IDDKVAHPETFLDFCVEVSKEIDEHLLN 169

[Close]

BLAST of Lsi05G003220 vs. Swiss-Prot
Match: KIRO_ACTDE (Kirola OS=Actinidia deliciosa PE=1 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 1.4e-11
Identity = 42/144 (29.17%), Postives = 77/144 (53.47%), Query Frame = 1


Query: 6   KISEQVKLKSSAHKFYEFFKNNMDYVFVQMFPEIYKSCKVVEGNGFSHGSVIHLKYNAGR 65
           K+ +QV++ S    FYE F+  + Y+  +M P   +   ++EGN  + GSVI  KY    
Sbjct: 6   KMVKQVEILSDGIVFYEIFRYRL-YLISEMSPVNIQGVDLLEGNWGTVGSVIFFKYTIDG 65

Query: 66  PEEVKERL--AIDDANKSITFECIEGDPLRDFEVIKLKFEVFENGSNGSSVNWSLEFVKA 125
            E+  + +  AID+  KS+TF+ +EGD +  ++   +  +V   G + +SV W+  + K 
Sbjct: 66  KEKTAKDIVEAIDEETKSVTFKIVEGDLMELYKTFIIIVQVDTKGEH-NSVTWTFHYEKL 125

Query: 126 NEDVAAPHHYLLCVAKVGKGLDDF 148
            EDV  P+  +    ++ K ++ +
Sbjct: 126 KEDVEEPNTLMNFCIEITKDIETY 147

[Close]

BLAST of Lsi05G003220 vs. TrEMBL
Match: A0A0A0LCM8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G333840 PE=4 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 2.2e-72
Identity = 128/151 (84.77%), Postives = 143/151 (94.70%), Query Frame = 1


Query: 1   MAQIAKISEQVKLKSSAHKFYEFFKNNMDYVFVQMFPEIYKSCKVVEGNGFSHGSVIHLK 60
           MAQI +ISEQV +KSSAHKFY+FFKN MDYVFVQMFPEIYKSCKVVEGNGFS GS+IHLK
Sbjct: 1   MAQICEISEQVNIKSSAHKFYQFFKNKMDYVFVQMFPEIYKSCKVVEGNGFSDGSIIHLK 60

Query: 61  YNAGRPEEVKERLAIDDANKSITFECIEGDPLRDFEVIKLKFEVFENGSNGSSVNWSLEF 120
           +NAG+PEEVKERLAIDDANKSITFEC+EGDPLR+FEV+KLKF+V ENG+NG +VNWS+EF
Sbjct: 61  FNAGKPEEVKERLAIDDANKSITFECLEGDPLRNFEVLKLKFQVLENGNNGGTVNWSIEF 120

Query: 121 VKANEDVAAPHHYLLCVAKVGKGLDDFLCNN 152
           VKANEDVA+PHHYLLCV KV KGLDD+LCNN
Sbjct: 121 VKANEDVASPHHYLLCVTKVAKGLDDYLCNN 151

[Close]

BLAST of Lsi05G003220 vs. TrEMBL
Match: A0A0A0LA22_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G331340 PE=4 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 7.1e-55
Identity = 109/152 (71.71%), Postives = 121/152 (79.61%), Query Frame = 1


Query: 1   MAQIAKISEQVKLKSSAHKFYEFFKNNMDYVFVQMFPEIYKSCKVVEGNGFSHGSVIHLK 60
           MAQIAKISEQV+LK S HKFY+FF N MD V +QMFP I  S K++EGNGF+HGSVIHLK
Sbjct: 15  MAQIAKISEQVQLKCSGHKFYDFFTNKMDSV-IQMFPHIVTSYKILEGNGFAHGSVIHLK 74

Query: 61  YNAGRPEEVKERLAIDDANKSITFECIEGDPLRDFEVIKLKFEVF-ENGSNGSSVNWSLE 120
           YN G P E+KERLA DDANKSI FE  EGD  RDFEV K+K +V  E GSNGSSVNWS+E
Sbjct: 75  YNIGGPAEIKERLAFDDANKSIAFEVFEGDLFRDFEVFKMKMQVINEKGSNGSSVNWSIE 134

Query: 121 FVKANEDVAAPHHYLLCVAKVGKGLDDFLCNN 152
           FVK NEDVAAPHHYL   A+  K LDD+LCNN
Sbjct: 135 FVKENEDVAAPHHYLTIAAQTSKTLDDYLCNN 165

[Close]

BLAST of Lsi05G003220 vs. TrEMBL
Match: A0A0A0L9D7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G219190 PE=4 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 1.3e-53
Identity = 107/152 (70.39%), Postives = 123/152 (80.92%), Query Frame = 1


Query: 1   MAQIAKISEQVKLKSSAHKFYEFFKNNMDYVFVQMFPEIYKSCKVVEGNGFSHGSVIHLK 60
           MAQIAKISEQ++LKSSAHKFY+FF N MD + +QMFP I+ S K++EGNGF+H SVIH+K
Sbjct: 1   MAQIAKISEQLQLKSSAHKFYDFFTNKMDSL-IQMFPHIFTSYKILEGNGFAHASVIHIK 60

Query: 61  YNAGRPEEVKERLAIDDANKSITFECIEGDPLRDFEVIKLKFEV-FENGSNGSSVNWSLE 120
           YN G P E+KERLA DDANKSI FE  EGD LRDFEV K+K +V  E GSNGS VNWS+E
Sbjct: 61  YNIGGPAEMKERLAFDDANKSIAFEVFEGDLLRDFEVFKMKMQVNNEKGSNGSLVNWSIE 120

Query: 121 FVKANEDVAAPHHYLLCVAKVGKGLDDFLCNN 152
           FVKANEDVAAPH YL   A+  K LDD+LCNN
Sbjct: 121 FVKANEDVAAPHQYLTIAAQTSKTLDDYLCNN 151

[Close]

BLAST of Lsi05G003220 vs. TrEMBL
Match: A0A0A0L771_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G337350 PE=4 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 8.1e-35
Identity = 84/154 (54.55%), Postives = 108/154 (70.13%), Query Frame = 1


Query: 1   MAQIAKISEQVKLKSSAHKFYEFFKNNMDYVFVQMFPE-IYKSCKVVEGNGFSHGSVIHL 60
           MAQI  ISE+VKLK+   KFY FF   MD V  QMFP+ I  + + VEGN F+HG+VIH 
Sbjct: 1   MAQICSISEEVKLKACGQKFYHFFLTKMD-VLNQMFPQNINCTYEFVEGNSFTHGTVIHW 60

Query: 61  KYN-AGRPEEVKERLAIDDANKSITFECIEGDPLRDFEVIKLKFEVFENGSNG-SSVNWS 120
           KY+  G  EE K RL +D+ NKSI  EC+EGD L++F+V K+K +V ++   G +SV W 
Sbjct: 61  KYDFGGGLEESKVRLTVDEPNKSIAMECLEGDVLKEFQVFKVKVQVKDSDVVGVNSVKWC 120

Query: 121 LEFVKANEDVAAPHHYLLCVAKVGKGLDDFLCNN 152
           +EFVKANEDVA P+++L C  KV K LD +LCNN
Sbjct: 121 VEFVKANEDVAPPNNHLKCGIKVCKDLDAYLCNN 153

[Close]

BLAST of Lsi05G003220 vs. TrEMBL
Match: A0A0A0L7N6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G331330 PE=4 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 3.1e-34
Identity = 78/154 (50.65%), Postives = 111/154 (72.08%), Query Frame = 1


Query: 1   MAQIAKISEQVKLKSSAHKFYEFFKNNMDYVFVQMFPEIYKSCKVVEGNGFSHGSVIHLK 60
           MAQIAKIS+QV+LK    KFY+FFKN MD++  ++FP+ ++S K+VEGN  +HGSV   K
Sbjct: 1   MAQIAKISDQVQLKCCGKKFYDFFKNKMDHL-PRVFPQYFESYKLVEGNSLTHGSVSFWK 60

Query: 61  YNAGRPE--EVKERLAIDDANKSITFECIEGDPLRDFEVIKLKFEVFENGSNG-SSVNWS 120
           Y+ G     EVK ++ +D+ NK+I +EC+EGD  +DF++  +K +V + G+NG SSVNW 
Sbjct: 61  YDFGFGSSIEVKMKVLVDEPNKTIIYECLEGDLFKDFDIFNVKIKVNDGGNNGNSSVNWC 120

Query: 121 LEFVKANEDVAAPHHYLLCVAKVGKGLDDFLCNN 152
           LE+VKANE+V  P++YL    K+ K +D FL NN
Sbjct: 121 LEYVKANENVDPPNNYLQFGLKLCKNVDAFLGNN 153

[Close]

BLAST of Lsi05G003220 vs. TAIR10
Match: AT1G70850.1 (AT1G70850.1 MLP-like protein 34)

HSP 1 Score: 79.0 bits (193), Expect = 2.9e-15
Identity = 47/145 (32.41%), Postives = 81/145 (55.86%), Query Frame = 1


Query: 7   ISEQVKLKSSAHKFYEFFKNNMDYVFVQMFPEIYKSCKVVEGNGFSHGSVIHLKY-NAGR 66
           +  +V++K+SA KF+  F     +V  +  P   +SC + EG+  + GS++   Y + G 
Sbjct: 169 LETEVEIKASAEKFHHMFAGKPHHVS-KATPGNIQSCDLHEGDWGTVGSIVFWNYVHDGE 228

Query: 67  PEEVKERL-AIDDANKSITFECIEGDPLRDFEVIKLKFEVF-ENGSNGSSVNWSLEFVKA 126
            +  KER+ A+D     ITF  IEGD +++++   +  +V  ++G +GS V+W  E+ K 
Sbjct: 229 AKVAKERIEAVDPEKNLITFRVIEGDLMKEYKSFVITIQVTPKHGGSGSVVHWHFEYEKI 288

Query: 127 NEDVAAPHHYLLCVAKVGKGLDDFL 149
           NE+VA P   L    +V K +D+ L
Sbjct: 289 NEEVAHPETLLQFAVEVSKEIDEHL 312

[Close]

BLAST of Lsi05G003220 vs. TAIR10
Match: AT1G70840.1 (AT1G70840.1 MLP-like protein 31)

HSP 1 Score: 73.6 bits (179), Expect = 1.2e-13
Identity = 45/148 (30.41%), Postives = 80/148 (54.05%), Query Frame = 1


Query: 6   KISEQVKLKSSAHKFYEFFKNNMDYVFVQMFPEIYKSCKVVEGNGFSHGSVIHLKY-NAG 65
           K+   +++K+SA KF+  F     +V  +  P   + C++ EG+    GS++   Y + G
Sbjct: 23  KLETDIEIKASAGKFHHMFAGRPHHVS-KATPGKIQGCELHEGDWGKVGSIVFWNYVHDG 82

Query: 66  RPEEVKERL-AIDDANKSITFECIEGDPLRDFEVIKLKFEVF-ENGSNGSSVNWSLEFVK 125
             +  KER+ A++     ITF  IEGD L++++   +  +V  + G  GS V+W +E+ K
Sbjct: 83  EAKVAKERIEAVEPEKNLITFRVIEGDLLKEYKSFVITIQVTPKRGGPGSVVHWHVEYEK 142

Query: 126 ANEDVAAPHHYLLCVAKVGKGLDDFLCN 151
            ++ VA P  +L    +V K +D+ L N
Sbjct: 143 IDDKVAHPETFLDFCVEVSKEIDEHLLN 169

[Close]

BLAST of Lsi05G003220 vs. TAIR10
Match: AT5G28010.1 (AT5G28010.1 Polyketide cyclase/dehydrase and lipid transport superfamily protein)

HSP 1 Score: 68.9 bits (167), Expect = 3.0e-12
Identity = 41/148 (27.70%), Postives = 79/148 (53.38%), Query Frame = 1


Query: 4   IAKISEQVKLKSSAHKFYEFFKNNMDYVFVQMFPEIYKSCKVVEGNGFSHGSVIHLKY-N 63
           + K+  +V++K+ A  FY  +     +V  +  P   +SC + +G   + GS+++  Y +
Sbjct: 16  LGKLEVEVEIKAPAAIFYHIYAGRPHHV-AKATPRNVQSCDLHDGEWGTVGSIVYWNYVH 75

Query: 64  AGRPEEVKERLA-IDDANKSITFECIEGDPLRDFEVIKLKFEVF-ENGSNGSSVNWSLEF 123
            G+ +  KER+  ++   K I F  IEGD + +++   +  +V  + G  GS V W +E+
Sbjct: 76  EGQAKVAKERIELVEPEKKLIKFRVIEGDVMAEYKSFLITIQVTPKEGGTGSVVKWHIEY 135

Query: 124 VKANEDVAAPHHYLLCVAKVGKGLDDFL 149
            K +E+V  P + L   A++ K +D+ L
Sbjct: 136 EKIDENVPHPENLLPFFAEMTKEIDEHL 162

[Close]

BLAST of Lsi05G003220 vs. TAIR10
Match: AT2G01520.1 (AT2G01520.1 MLP-like protein 328)

HSP 1 Score: 65.5 bits (158), Expect = 3.3e-11
Identity = 39/147 (26.53%), Postives = 71/147 (48.30%), Query Frame = 1


Query: 1   MAQIAKISEQVKLKSSAHKFYEFFKNNMDYVFVQMFPEIYKSCKVVEGNGFSHGSVIHLK 60
           MA       +V LK SA K Y+ +++  +++F        +   + +G   SHG++    
Sbjct: 1   MATSGTYVTEVPLKGSAEKHYKRWRSE-NHLFPDAIGHHIQGVTIHDGEWDSHGAIKIWN 60

Query: 61  YNA-GRPEEVKERLAIDDANKSITFECIEGDPLRDFEVIKLKFEVFENGSNGSSVNWSLE 120
           Y   G+PE  KER  IDD N ++TF  +EG  +   +V  + F+  +   +      ++ 
Sbjct: 61  YTCDGKPEVFKERREIDDENMAVTFRGLEGHVMEQLKVYDVIFQFIQKSPDDIICKITMI 120

Query: 121 FVKANEDVAAPHHYLLCVAKVGKGLDD 147
           + K N+D+  P +Y+  V  +   +DD
Sbjct: 121 WEKQNDDMPEPSNYMKFVKSLAADMDD 146

[Close]

BLAST of Lsi05G003220 vs. TAIR10
Match: AT1G24020.1 (AT1G24020.1 MLP-like protein 423)

HSP 1 Score: 65.5 bits (158), Expect = 3.3e-11
Identity = 44/142 (30.99%), Postives = 75/142 (52.82%), Query Frame = 1


Query: 10  QVKLKSSAHKFYEFFKNNMDYVFVQMFPEIYKSCKVVEGNGFSHGSVIHLKYNAGRP--E 69
           +V++KS A KF+    + ++ +F + FP  YK+ +V+ G+G + GS+  + Y  G P  +
Sbjct: 10  EVEVKSPAEKFWVALGDGIN-LFPKAFPNDYKTIQVLAGDGNAPGSIRLITYGEGSPLVK 69

Query: 70  EVKERL-AIDDANKSITFECIEGDPLRDFEVIKLKFEVFENGSNGSSVNWSLEFVKANED 129
              ER+ A+D  NKS+++  I G+ L  ++  K    V      GS + WS EF K   +
Sbjct: 70  ISAERIEAVDLENKSMSYSIIGGEMLEYYKTFKGTITVIPK-DGGSLLKWSGEFEKTAHE 129

Query: 130 VAAPHHYLLCVAKVGKGLDDFL 149
           +  PH       K  K +D++L
Sbjct: 130 IDDPHVIKDFAVKNFKEIDEYL 149

[Close]

BLAST of Lsi05G003220 vs. NCBI nr
Match: gi|659114440|ref|XP_008457055.1| (PREDICTED: MLP-like protein 34 [Cucumis melo])

HSP 1 Score: 283.1 bits (723), Expect = 2.8e-73
Identity = 129/151 (85.43%), Postives = 144/151 (95.36%), Query Frame = 1


Query: 1   MAQIAKISEQVKLKSSAHKFYEFFKNNMDYVFVQMFPEIYKSCKVVEGNGFSHGSVIHLK 60
           MAQI KISEQV +KSSAHK+Y+FFKN MDYV VQMFPEIYKSCKVVEGNG+SHGS+IHLK
Sbjct: 1   MAQICKISEQVNIKSSAHKYYQFFKNKMDYVLVQMFPEIYKSCKVVEGNGYSHGSIIHLK 60

Query: 61  YNAGRPEEVKERLAIDDANKSITFECIEGDPLRDFEVIKLKFEVFENGSNGSSVNWSLEF 120
           YNAGRPEE+KERLAIDDANKSITFEC+EGD LRDFEV+KLKF+VFENG+NG +VNWS+EF
Sbjct: 61  YNAGRPEEMKERLAIDDANKSITFECLEGDALRDFEVLKLKFQVFENGNNGGTVNWSIEF 120

Query: 121 VKANEDVAAPHHYLLCVAKVGKGLDDFLCNN 152
           VKANEDVA+PHHYLLCV+KV KGLDD+LCNN
Sbjct: 121 VKANEDVASPHHYLLCVSKVAKGLDDYLCNN 151

[Close]

BLAST of Lsi05G003220 vs. NCBI nr
Match: gi|449464138|ref|XP_004149786.1| (PREDICTED: kirola [Cucumis sativus])

HSP 1 Score: 279.6 bits (714), Expect = 3.1e-72
Identity = 128/151 (84.77%), Postives = 143/151 (94.70%), Query Frame = 1


Query: 1   MAQIAKISEQVKLKSSAHKFYEFFKNNMDYVFVQMFPEIYKSCKVVEGNGFSHGSVIHLK 60
           MAQI +ISEQV +KSSAHKFY+FFKN MDYVFVQMFPEIYKSCKVVEGNGFS GS+IHLK
Sbjct: 1   MAQICEISEQVNIKSSAHKFYQFFKNKMDYVFVQMFPEIYKSCKVVEGNGFSDGSIIHLK 60

Query: 61  YNAGRPEEVKERLAIDDANKSITFECIEGDPLRDFEVIKLKFEVFENGSNGSSVNWSLEF 120
           +NAG+PEEVKERLAIDDANKSITFEC+EGDPLR+FEV+KLKF+V ENG+NG +VNWS+EF
Sbjct: 61  FNAGKPEEVKERLAIDDANKSITFECLEGDPLRNFEVLKLKFQVLENGNNGGTVNWSIEF 120

Query: 121 VKANEDVAAPHHYLLCVAKVGKGLDDFLCNN 152
           VKANEDVA+PHHYLLCV KV KGLDD+LCNN
Sbjct: 121 VKANEDVASPHHYLLCVTKVAKGLDDYLCNN 151

[Close]

BLAST of Lsi05G003220 vs. NCBI nr
Match: gi|778680829|ref|XP_004151203.2| (PREDICTED: major latex protein 149-like [Cucumis sativus])

HSP 1 Score: 221.5 bits (563), Expect = 1.0e-54
Identity = 109/152 (71.71%), Postives = 121/152 (79.61%), Query Frame = 1


Query: 1   MAQIAKISEQVKLKSSAHKFYEFFKNNMDYVFVQMFPEIYKSCKVVEGNGFSHGSVIHLK 60
           MAQIAKISEQV+LK S HKFY+FF N MD V +QMFP I  S K++EGNGF+HGSVIHLK
Sbjct: 1   MAQIAKISEQVQLKCSGHKFYDFFTNKMDSV-IQMFPHIVTSYKILEGNGFAHGSVIHLK 60

Query: 61  YNAGRPEEVKERLAIDDANKSITFECIEGDPLRDFEVIKLKFEVF-ENGSNGSSVNWSLE 120
           YN G P E+KERLA DDANKSI FE  EGD  RDFEV K+K +V  E GSNGSSVNWS+E
Sbjct: 61  YNIGGPAEIKERLAFDDANKSIAFEVFEGDLFRDFEVFKMKMQVINEKGSNGSSVNWSIE 120

Query: 121 FVKANEDVAAPHHYLLCVAKVGKGLDDFLCNN 152
           FVK NEDVAAPHHYL   A+  K LDD+LCNN
Sbjct: 121 FVKENEDVAAPHHYLTIAAQTSKTLDDYLCNN 151

[Close]

BLAST of Lsi05G003220 vs. NCBI nr
Match: gi|700202691|gb|KGN57824.1| (hypothetical protein Csa_3G331340 [Cucumis sativus])

HSP 1 Score: 221.5 bits (563), Expect = 1.0e-54
Identity = 109/152 (71.71%), Postives = 121/152 (79.61%), Query Frame = 1


Query: 1   MAQIAKISEQVKLKSSAHKFYEFFKNNMDYVFVQMFPEIYKSCKVVEGNGFSHGSVIHLK 60
           MAQIAKISEQV+LK S HKFY+FF N MD V +QMFP I  S K++EGNGF+HGSVIHLK
Sbjct: 15  MAQIAKISEQVQLKCSGHKFYDFFTNKMDSV-IQMFPHIVTSYKILEGNGFAHGSVIHLK 74

Query: 61  YNAGRPEEVKERLAIDDANKSITFECIEGDPLRDFEVIKLKFEVF-ENGSNGSSVNWSLE 120
           YN G P E+KERLA DDANKSI FE  EGD  RDFEV K+K +V  E GSNGSSVNWS+E
Sbjct: 75  YNIGGPAEIKERLAFDDANKSIAFEVFEGDLFRDFEVFKMKMQVINEKGSNGSSVNWSIE 134

Query: 121 FVKANEDVAAPHHYLLCVAKVGKGLDDFLCNN 152
           FVK NEDVAAPHHYL   A+  K LDD+LCNN
Sbjct: 135 FVKENEDVAAPHHYLTIAAQTSKTLDDYLCNN 165

[Close]

BLAST of Lsi05G003220 vs. NCBI nr
Match: gi|778680249|ref|XP_011651277.1| (PREDICTED: major latex protein 146-like [Cucumis sativus])

HSP 1 Score: 217.2 bits (552), Expect = 1.9e-53
Identity = 107/152 (70.39%), Postives = 123/152 (80.92%), Query Frame = 1


Query: 1   MAQIAKISEQVKLKSSAHKFYEFFKNNMDYVFVQMFPEIYKSCKVVEGNGFSHGSVIHLK 60
           MAQIAKISEQ++LKSSAHKFY+FF N MD + +QMFP I+ S K++EGNGF+H SVIH+K
Sbjct: 1   MAQIAKISEQLQLKSSAHKFYDFFTNKMDSL-IQMFPHIFTSYKILEGNGFAHASVIHIK 60

Query: 61  YNAGRPEEVKERLAIDDANKSITFECIEGDPLRDFEVIKLKFEV-FENGSNGSSVNWSLE 120
           YN G P E+KERLA DDANKSI FE  EGD LRDFEV K+K +V  E GSNGS VNWS+E
Sbjct: 61  YNIGGPAEMKERLAFDDANKSIAFEVFEGDLLRDFEVFKMKMQVNNEKGSNGSLVNWSIE 120

Query: 121 FVKANEDVAAPHHYLLCVAKVGKGLDDFLCNN 152
           FVKANEDVAAPH YL   A+  K LDD+LCNN
Sbjct: 121 FVKANEDVAAPHQYLTIAAQTSKTLDDYLCNN 151

The following BLAST results are available for this feature:

BLAST of Lsi05G003220 vs. Swiss-Prot


Match Name	E-value	Identity	Description
MLP34_ARATH	5.1e-14	32.41	MLP-like protein 34 OS=Arabidopsis thaliana GN=MLP34 PE=2 SV=1	[more]
ML146_PAPSO	3.3e-13	29.05	Major latex protein 146 OS=Papaver somniferum GN=MLP146 PE=2 SV=1	[more]
MLP15_PAPSO	1.6e-12	32.21	Major latex protein 15 OS=Papaver somniferum GN=MLP15 PE=2 SV=2	[more]
MLP31_ARATH	2.1e-12	30.41	MLP-like protein 31 OS=Arabidopsis thaliana GN=MLP31 PE=2 SV=2	[more]
KIRO_ACTDE	1.4e-11	29.17	Kirola OS=Actinidia deliciosa PE=1 SV=1	[more]

BLAST of Lsi05G003220 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LCM8_CUCSA	2.2e-72	84.77	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G333840 PE=4 SV=1	[more]
A0A0A0LA22_CUCSA	7.1e-55	71.71	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G331340 PE=4 SV=1	[more]
A0A0A0L9D7_CUCSA	1.3e-53	70.39	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G219190 PE=4 SV=1	[more]
A0A0A0L771_CUCSA	8.1e-35	54.55	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G337350 PE=4 SV=1	[more]
A0A0A0L7N6_CUCSA	3.1e-34	50.65	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G331330 PE=4 SV=1	[more]

BLAST of Lsi05G003220 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G70850.1	2.9e-15	32.41	MLP-like protein 34	[more]
AT1G70840.1	1.2e-13	30.41	MLP-like protein 31	[more]
AT5G28010.1	3.0e-12	27.70	Polyketide cyclase/dehydrase and lipid transport superfamily protein	[more]
AT2G01520.1	3.3e-11	26.53	MLP-like protein 328	[more]
AT1G24020.1	3.3e-11	30.99	MLP-like protein 423	[more]

BLAST of Lsi05G003220 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659114440\|ref\|XP_008457055.1\|	2.8e-73	85.43	PREDICTED: MLP-like protein 34 [Cucumis melo]	[more]
gi\|449464138\|ref\|XP_004149786.1\|	3.1e-72	84.77	PREDICTED: kirola [Cucumis sativus]	[more]
gi\|778680829\|ref\|XP_004151203.2\|	1.0e-54	71.71	PREDICTED: major latex protein 149-like [Cucumis sativus]	[more]
gi\|700202691\|gb\|KGN57824.1\|	1.0e-54	71.71	hypothetical protein Csa_3G331340 [Cucumis sativus]	[more]
gi\|778680249\|ref\|XP_011651277.1\|	1.9e-53	70.39	PREDICTED: major latex protein 146-like [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0009607	response to biotic stimulus
GO:0006952	defense response

Vocabulary: **INTERPRO**
Term	Definition
IPR023393	START-like_dom_sf
IPR000916	Bet_v_I/MLP

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0009607	response to biotic stimulus
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G003220.1	Lsi05G003220.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000916	Bet v I/Major latex protein	PFAM	PF00407	Bet_v_1	coord: 5..150 score: 1.2
IPR000916	Bet v I/Major latex protein	SMART	SM01037	Bet_v_1_2	coord: 2..151 score: 1.3
IPR023393	START-like domain	GENE3D	G3DSA:3.30.530.20		coord: 1..151 score: 3.1
None	No IPR available	PANTHER	PTHR31907	FAMILY NOT NAMED	coord: 2..148 score: 2.4
None	No IPR available	PANTHER	PTHR31907:SF2	SUBFAMILY NOT NAMED	coord: 2..148 score: 2.4
None	No IPR available	unknown	SSF55961	Bet v1-like	coord: 5..145 score: 6.53

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi05G003220	Silver-seed gourd	carlsiB464
Lsi05G003220	Silver-seed gourd	carlsiB700
Lsi05G003220	Cucumber (Chinese Long) v3	cuclsiB231
Lsi05G003220	Watermelon (97103) v2	lsiwmbB329
Lsi05G003220	Wax gourd	lsiwgoB447
Lsi05G003220	Cucumber (Gy14) v1	cgylsiB607
Lsi05G003220	Cucurbita maxima (Rimu)	cmalsiB249
Lsi05G003220	Cucurbita maxima (Rimu)	cmalsiB440
Lsi05G003220	Cucurbita moschata (Rifu)	cmolsiB237
Lsi05G003220	Cucurbita moschata (Rifu)	cmolsiB430
Lsi05G003220	Cucurbita pepo (Zucchini)	cpelsiB470
Lsi05G003220	Cucurbita pepo (Zucchini)	cpelsiB511
Lsi05G003220	Wild cucumber (PI 183967)	cpilsiB210
Lsi05G003220	Cucumber (Chinese Long) v2	culsiB212
Lsi05G003220	Melon (DHL92) v3.5.1	lsimeB368
Lsi05G003220	Watermelon (Charleston Gray)	lsiwcgB332
Lsi05G003220	Watermelon (97103) v1	lsiwmB361
Lsi05G003220	Cucumber (Gy14) v2	cgyblsiB192
Lsi05G003220	Cucumber (Gy14) v2	cgyblsiB199
Lsi05G003220	Melon (DHL92) v3.6.1	lsimedB384