Lsi04G022000 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi04G022000
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	N-methyl-L-tryptophan oxidase
Location	chr04 : 29050757 .. 29051989 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGATTCCGGCACTCAATTCGATGTAATCGTCGTTGGCGCCGGCGTAATGGGTAGTTCCACGGCGTACCATCTCGCTAAAACAGGGAACAGAATTCTGATTCTCGAGCAATTCGATTTTCTTCATCACAGAGGCTCTTCTCATGGCGAATCTCGTACCATACGCGCCACTTATCCGGAGGATTACTACCACGGCCTCGTTATGGAATCTTACGAGCTCTGGCGGATGACGGAGGCGGAAATTGGCTACAGAGTTTATTTTCCGGCGGAGCAGCTCGATATCGGCCCTTCCGACGACAAAAGTCTCGCCGCCGTCGTCGAGACCTGCCGGAAGCATTCGATCCCTCATCTGGTCCTGGATCGAGGACAACTGGCGGAGAAGTACTCCGGCAGAGTGGAGATTCCGGCGGACTGGGTGGCGGTGTGGAGCAAGTACGGCGGCGTAATTAAGCCAACGAAGGCGGTTTCGATGTTCCAAAGTTTGGCTTACAAAAACGGCGCCGTTTTGAAGGACAATGCGGAAGTGGTGGAGATTAAGAGAGATGGGAGTAGTGGAGGAATAGTTGTTTCAACAGCCAATGGGCAGAGTTTTAGAGGAAAAAAATGTGTGGTGACAGTTGGAGCTTGGGCTAGAAAGTTATTTAAATCAGTTGGTGGGATTGAATTGCCAATTCAGCCATTGGAGGCTACTGTATCCTACTGGAGGATCAAGGAAGGGGTGGAGGCGGCCGAGTATGCAATCGGAGGGGACTTTCCGACATTTGCTAGCTATGGTGACCCATATATTTACGGGACACCATCGTTGGAGTTTCCAGGATTGATCAAAGTGGCTATACACGGCGGGCATCAGTGCGACCCGGACAAGCGATCGTGGGGGAATGGGGAGCGGCTACCGATAGCTACATTAAAGGAGTGGATAGAGGAGAGGTTTGGGGGGAGGGTGGATTCAAGTGAGCCGGTGTCAACGCAGTTGTGTATGTACTCAATGACGCCAGATGAGGACTTTGTGATTGATTTTTTGGGAGGGGAATTTGAAAAGGATGTAGTGATCGGTGGCGGGTTTTCAGGGCATGGGTTCAAAATGTCACCGGCGGTCGGGAGGATACTGGCGGAGCTTGCATTGAATGGAGAAGCGGAAGGAACGGAGCTGAAGTATTTTAAGATGGCAAGGTTTGAGGAGAATCCAAAAGGTAATGTCAAGAGCTTTGCAGATCAAGTAAAGCTTCACTAG

mRNA sequence

ATGGCTGATTCCGGCACTCAATTCGATGTAATCGTCGTTGGCGCCGGCGTAATGGGTAGTTCCACGGCGTACCATCTCGCTAAAACAGGGAACAGAATTCTGATTCTCGAGCAATTCGATTTTCTTCATCACAGAGGCTCTTCTCATGGCGAATCTCGTACCATACGCGCCACTTATCCGGAGGATTACTACCACGGCCTCGTTATGGAATCTTACGAGCTCTGGCGGATGACGGAGGCGGAAATTGGCTACAGAGTTTATTTTCCGGCGGAGCAGCTCGATATCGGCCCTTCCGACGACAAAAGTCTCGCCGCCGTCGTCGAGACCTGCCGGAAGCATTCGATCCCTCATCTGGTCCTGGATCGAGGACAACTGGCGGAGAAGTACTCCGGCAGAGTGGAGATTCCGGCGGACTGGGTGGCGGTGTGGAGCAAGTACGGCGGCGTAATTAAGCCAACGAAGGCGGTTTCGATGTTCCAAAGTTTGGCTTACAAAAACGGCGCCGTTTTGAAGGACAATGCGGAAGTGGTGGAGATTAAGAGAGATGGGAGTAGTGGAGGAATAGTTGTTTCAACAGCCAATGGGCAGAGTTTTAGAGGAAAAAAATGTGTGGTGACAGTTGGAGCTTGGGCTAGAAAGTTATTTAAATCAGTTGGTGGGATTGAATTGCCAATTCAGCCATTGGAGGCTACTGTATCCTACTGGAGGATCAAGGAAGGGGTGGAGGCGGCCGAGTATGCAATCGGAGGGGACTTTCCGACATTTGCTAGCTATGGTGACCCATATATTTACGGGACACCATCGTTGGAGTTTCCAGGATTGATCAAAGTGGCTATACACGGCGGGCATCAGTGCGACCCGGACAAGCGATCGTGGGGGAATGGGGAGCGGCTACCGATAGCTACATTAAAGGAGTGGATAGAGGAGAGGTTTGGGGGGAGGGTGGATTCAAGTGAGCCGGTGTCAACGCAGTTGTGTATGTACTCAATGACGCCAGATGAGGACTTTGTGATTGATTTTTTGGGAGGGGAATTTGAAAAGGATGTAGTGATCGGTGGCGGGTTTTCAGGGCATGGGTTCAAAATGTCACCGGCGGTCGGGAGGATACTGGCGGAGCTTGCATTGAATGGAGAAGCGGAAGGAACGGAGCTGAAGTATTTTAAGATGGCAAGGTTTGAGGAGAATCCAAAAGGTAATGTCAAGAGCTTTGCAGATCAAGTAAAGCTTCACTAG

Coding sequence (CDS)

ATGGCTGATTCCGGCACTCAATTCGATGTAATCGTCGTTGGCGCCGGCGTAATGGGTAGTTCCACGGCGTACCATCTCGCTAAAACAGGGAACAGAATTCTGATTCTCGAGCAATTCGATTTTCTTCATCACAGAGGCTCTTCTCATGGCGAATCTCGTACCATACGCGCCACTTATCCGGAGGATTACTACCACGGCCTCGTTATGGAATCTTACGAGCTCTGGCGGATGACGGAGGCGGAAATTGGCTACAGAGTTTATTTTCCGGCGGAGCAGCTCGATATCGGCCCTTCCGACGACAAAAGTCTCGCCGCCGTCGTCGAGACCTGCCGGAAGCATTCGATCCCTCATCTGGTCCTGGATCGAGGACAACTGGCGGAGAAGTACTCCGGCAGAGTGGAGATTCCGGCGGACTGGGTGGCGGTGTGGAGCAAGTACGGCGGCGTAATTAAGCCAACGAAGGCGGTTTCGATGTTCCAAAGTTTGGCTTACAAAAACGGCGCCGTTTTGAAGGACAATGCGGAAGTGGTGGAGATTAAGAGAGATGGGAGTAGTGGAGGAATAGTTGTTTCAACAGCCAATGGGCAGAGTTTTAGAGGAAAAAAATGTGTGGTGACAGTTGGAGCTTGGGCTAGAAAGTTATTTAAATCAGTTGGTGGGATTGAATTGCCAATTCAGCCATTGGAGGCTACTGTATCCTACTGGAGGATCAAGGAAGGGGTGGAGGCGGCCGAGTATGCAATCGGAGGGGACTTTCCGACATTTGCTAGCTATGGTGACCCATATATTTACGGGACACCATCGTTGGAGTTTCCAGGATTGATCAAAGTGGCTATACACGGCGGGCATCAGTGCGACCCGGACAAGCGATCGTGGGGGAATGGGGAGCGGCTACCGATAGCTACATTAAAGGAGTGGATAGAGGAGAGGTTTGGGGGGAGGGTGGATTCAAGTGAGCCGGTGTCAACGCAGTTGTGTATGTACTCAATGACGCCAGATGAGGACTTTGTGATTGATTTTTTGGGAGGGGAATTTGAAAAGGATGTAGTGATCGGTGGCGGGTTTTCAGGGCATGGGTTCAAAATGTCACCGGCGGTCGGGAGGATACTGGCGGAGCTTGCATTGAATGGAGAAGCGGAAGGAACGGAGCTGAAGTATTTTAAGATGGCAAGGTTTGAGGAGAATCCAAAAGGTAATGTCAAGAGCTTTGCAGATCAAGTAAAGCTTCACTAG

Protein sequence

MADSGTQFDVIVVGAGVMGSSTAYHLAKTGNRILILEQFDFLHHRGSSHGESRTIRATYPEDYYHGLVMESYELWRMTEAEIGYRVYFPAEQLDIGPSDDKSLAAVVETCRKHSIPHLVLDRGQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQSLAYKNGAVLKDNAEVVEIKRDGSSGGIVVSTANGQSFRGKKCVVTVGAWARKLFKSVGGIELPIQPLEATVSYWRIKEGVEAAEYAIGGDFPTFASYGDPYIYGTPSLEFPGLIKVAIHGGHQCDPDKRSWGNGERLPIATLKEWIEERFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGFKMSPAVGRILAELALNGEAEGTELKYFKMARFEENPKGNVKSFADQVKLH

Homology

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BLAST of Lsi04G022000 vs. Swiss-Prot
Match: SOX_ARATH (Probable sarcosine oxidase OS=Arabidopsis thaliana GN=At2g24580 PE=2 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 3.4e-161
Identity = 269/411 (65.45%), Postives = 328/411 (79.81%), Query Frame = 1


Query: 2   ADSGTQFDVIVVGAGVMGSSTAYHLAKTGNRILILEQFDFLHHRGSSHGESRTIRATYPE 61
           +D G +FDVIVVGAGVMGSS AY LAK G + L+LEQFDFLHHRGSSHGESRTIRATYPE
Sbjct: 4   SDDG-RFDVIVVGAGVMGSSAAYQLAKRGQKTLLLEQFDFLHHRGSSHGESRTIRATYPE 63

Query: 62  DYYHGLVMESYELWRMTEAEIGYRVYFPAEQLDIGPSDDKSLAAVVETCRKHSIPHLVLD 121
           DYY+ +V ES  LW   ++EIGY+V+FP +Q D+GP+D +SL +VV TC+KH + H V+D
Sbjct: 64  DYYYSMVSESTRLWAAAQSEIGYKVHFPTQQFDMGPADQQSLLSVVATCQKHGLAHRVMD 123

Query: 122 RGQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQSLAYKNGAVLKDNAEVVEIKR 181
              ++E +SGR+ IP +W+ V ++ GG+IKPTKAVSMFQ+LA  +GA+L+DN +V  IKR
Sbjct: 124 SHAVSEHFSGRISIPENWIGVSTELGGIIKPTKAVSMFQTLAIGHGAILRDNTKVANIKR 183

Query: 182 DGSSG-GIVVSTANGQSFRGKKCVVTVGAWARKLFKSVGGIELPIQPLEATVSYWRIKEG 241
           DG SG G++V T  G  F GKKC+VT GAW  KL K+V GI+ P++PLE TV YWRIKEG
Sbjct: 184 DGESGEGVIVCTVKGDKFYGKKCIVTAGAWISKLVKTVAGIDFPVEPLETTVCYWRIKEG 243

Query: 242 VEAAEYAIGGDFPTFASYGDPYIYGTPSLEFPGLIKVAIHGGHQCDPDKRSWGNGERLPI 301
            E  ++ I G+FPTFASYG PY+YGTPSLE+PGLIKVA+HGG+ CDPDKR WG G +L  
Sbjct: 244 HE-EKFTIDGEFPTFASYGAPYVYGTPSLEYPGLIKVAVHGGYWCDPDKRPWGPGVKL-- 303

Query: 302 ATLKEWIEERFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGF 361
             LKEWI+ERFGG VDS  PV+TQLCMYSMTPDEDFVIDFLGGEF +DVV+GGGFSGHGF
Sbjct: 304 EELKEWIKERFGGMVDSEGPVATQLCMYSMTPDEDFVIDFLGGEFGRDVVVGGGFSGHGF 363

Query: 362 KMSPAVGRILAELALNGEA--EGTELKYFKMARFEENPKGNVKSFADQVKL 410
           KM+PAVGRILA++A+  EA   G E+K F + RFE+NPKGN K + DQV L
Sbjct: 364 KMAPAVGRILADMAMEVEAGGGGVEMKQFSLRRFEDNPKGNAKEYPDQVIL 410

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BLAST of Lsi04G022000 vs. Swiss-Prot
Match: SOX_BOVIN (Peroxisomal sarcosine oxidase OS=Bos taurus GN=PIPOX PE=2 SV=2)

HSP 1 Score: 241.5 bits (615), Expect = 1.6e-62
Identity = 142/406 (34.98%), Postives = 212/406 (52.22%), Query Frame = 1


Query: 1   MADSGTQFDVIVVGAGVMGSSTAYHLAKTGNRILILEQFDFLHHRGSSHGESRTIRATYP 60
           MA     +D IV+GAG+ G  TAYHLAK   ++L+LEQF   H RGSSHG+SR IR  YP
Sbjct: 1   MAAQRELYDAIVIGAGIQGCFTAYHLAKHSKKVLLLEQFFLPHSRGSSHGQSRIIRRAYP 60

Query: 61  EDYYHGLVMESYELWRMTEAEIGYRVYFPAEQLDIGPSDDKSLAAVVETCRKHSIPHLVL 120
           ED+Y  ++ E Y LW   E E G ++Y     L +G  ++  L  +  T  +  + H  L
Sbjct: 61  EDFYTQMMAECYSLWAQLEHEAGTQLYRQTGLLLLGMKENPELKIIQATLSRQGVEHQCL 120

Query: 121 DRGQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQSLAYKNGAVLKDNAEVVEIK 180
              +L +++   + +    V +    GGV+   KA+   Q    + G ++ D  +VVEIK
Sbjct: 121 SSEELKQRFP-NIRLARGEVGLLEVSGGVLYADKALRALQDAIRQLGGIVHDGEKVVEIK 180

Query: 181 RDGSSGGIVVSTANGQSFRGKKCVVTVGAWARKLFKSVGGIELPIQPLEATVSYWRIKEG 240
               SG  V+     +S++ K  ++T G W  +L + +G  ELP+Q L   V YW+ K  
Sbjct: 181 ----SGLPVMVKTTSRSYQAKSLIITAGPWTNRLLRPLGA-ELPLQTLRINVCYWQEK-- 240

Query: 241 VEAAEYAIGGDFPTFASYG----DPYIYGTPSLEFPGLIKVAIHGGHQCDPDKRSW--GN 300
                Y++   FP F   G      +IYG PS E+PGL+KV  H G+  DP++R      
Sbjct: 241 -VPGSYSVSQAFPCFMGLGLSLAPHHIYGLPSREYPGLMKVCYHHGNNADPEERDCPAAF 300

Query: 301 GERLPIATLKEWIEERFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGG 360
            +   +  L  ++ +         EP   + CMY+ TPD  FV+D        ++VIG G
Sbjct: 301 SDIQDVHILSGFVRDHLPDL--QPEPAVMEHCMYTNTPDGHFVLD--RHPKYDNIVIGAG 360

Query: 361 FSGHGFKMSPAVGRILAELALNGEAEGTELKYFKMARFEENPKGNV 401
           FSGHGFK+SP VG+IL EL++       +L  F+++RF    K ++
Sbjct: 361 FSGHGFKLSPVVGKILYELSMK-LTPSYDLTPFRISRFPSLGKAHL 392

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BLAST of Lsi04G022000 vs. Swiss-Prot
Match: SOX_HUMAN (Peroxisomal sarcosine oxidase OS=Homo sapiens GN=PIPOX PE=1 SV=2)

HSP 1 Score: 238.8 bits (608), Expect = 1.0e-61
Identity = 139/404 (34.41%), Postives = 215/404 (53.22%), Query Frame = 1


Query: 1   MADSGTQFDVIVVGAGVMGSSTAYHLAKTGNRILILEQFDFLHHRGSSHGESRTIRATYP 60
           MA     +D IV+GAG+ G  TAYHLAK   RIL+LEQF   H RGSSHG+SR IR  Y 
Sbjct: 1   MAAQKDLWDAIVIGAGIQGCFTAYHLAKHRKRILLLEQFFLPHSRGSSHGQSRIIRKAYL 60

Query: 61  EDYYHGLVMESYELWRMTEAEIGYRVYFPAEQLDIGPSDDKSLAAVVETCRKHSIPHLVL 120
           ED+Y  ++ E Y++W   E E G +++     L +G  +++ L  +     +  + H  L
Sbjct: 61  EDFYTRMMHECYQIWAQLEHEAGTQLHRQTGLLLLGMKENQELKTIQANLSRQRVEHQCL 120

Query: 121 DRGQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQSLAYKNGAVLKDNAEVVEIK 180
              +L +++   + +P   V +    GGVI   KA+   Q    + G +++D  +VVEI 
Sbjct: 121 SSEELKQRFP-NIRLPRGEVGLLDNSGGVIYAYKALRALQDAIRQLGGIVRDGEKVVEI- 180

Query: 181 RDGSSGGIVVSTANGQSFRGKKCVVTVGAWARKLFKSVGGIELPIQPLEATVSYWRIKEG 240
              + G +V      +S++ K  V+T G W  +L + + GIE+P+Q L   V YWR    
Sbjct: 181 ---NPGLLVTVKTTSRSYQAKSLVITAGPWTNQLLRPL-GIEMPLQTLRINVCYWR---E 240

Query: 241 VEAAEYAIGGDFPTFASYG--DPYIYGTPSLEFPGLIKVAIHGGHQCDPDKRS--WGNGE 300
           +    Y +   FP F   G    +IYG P+ E+PGL+KV+ H G+  DP++R       +
Sbjct: 241 MVPGSYGVSQAFPCFLWLGLCPHHIYGLPTGEYPGLMKVSYHHGNHADPEERDCPTARTD 300

Query: 301 RLPIATLKEWIEERFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFS 360
              +  L  ++ +         EP   + CMY+ TPDE F++D        ++VIG GFS
Sbjct: 301 IGDVQILSSFVRDHLPDL--KPEPAVIESCMYTNTPDEQFILD--RHPKYDNIVIGAGFS 360

Query: 361 GHGFKMSPAVGRILAELALNGEAEGTELKYFKMARFEENPKGNV 401
           GHGFK++P VG+IL EL++       +L  F+++RF    K ++
Sbjct: 361 GHGFKLAPVVGKILYELSMK-LTPSYDLAPFRISRFPSLGKAHL 390

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BLAST of Lsi04G022000 vs. Swiss-Prot
Match: SOX_RABIT (Peroxisomal sarcosine oxidase OS=Oryctolagus cuniculus GN=PIPOX PE=1 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 8.8e-61
Identity = 137/404 (33.91%), Postives = 213/404 (52.72%), Query Frame = 1


Query: 1   MADSGTQFDVIVVGAGVMGSSTAYHLAKTGNRILILEQFDFLHHRGSSHGESRTIRATYP 60
           MA     +D IV+GAG+ G  T YHL K   RIL+LEQF   H RGSSHG+SR IR  Y 
Sbjct: 1   MAAQKDLWDAIVIGAGIQGCFTVYHLVKHRKRILLLEQFFLPHSRGSSHGQSRIIRKAYL 60

Query: 61  EDYYHGLVMESYELWRMTEAEIGYRVYFPAEQLDIGPSDDKSLAAVVETCRKHSIPHLVL 120
           ED+Y  ++ E Y++W   E E G +++     L +G  +++ L  +     +  + H  L
Sbjct: 61  EDFYTRMMHECYQIWAQLEHEAGTQLHRQTGLLLLGMKENQELKTIQANLSRQRVEHQCL 120

Query: 121 DRGQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQSLAYKNGAVLKDNAEVVEIK 180
              +L +++   + +P   V +    GGVI   KA+   Q    + G +++D  +VVEI 
Sbjct: 121 SSEELKQRFP-NIRLPRGEVGLLDNSGGVIYAYKALRALQDAIRQLGGIVRDGEKVVEI- 180

Query: 181 RDGSSGGIVVSTANGQSFRGKKCVVTVGAWARKLFKSVGGIELPIQPLEATVSYWRIKEG 240
              + G +V      +S++ K  V+T G W  +L + + GIE+P+Q L   V YWR    
Sbjct: 181 ---NPGLLVTVKTTSRSYQAKSLVITAGPWTNQLLRPL-GIEMPLQTLRINVCYWR---E 240

Query: 241 VEAAEYAIGGDFPTFASYG--DPYIYGTPSLEFPGLIKVAIHGGHQCDPDKRS--WGNGE 300
           +    Y +   FP F   G    +IYG P+ E+PGL+KV+ H G+  DP++R       +
Sbjct: 241 MVPGSYGVSQAFPCFLWLGLCPHHIYGLPTGEYPGLMKVSYHHGNHADPEERDCPTARTD 300

Query: 301 RLPIATLKEWIEERFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFS 360
              +  L  ++ +         EP   + CMY+ TPDE F++D        ++VIG GFS
Sbjct: 301 IGDVQILSSFVRDHLPDL--KPEPAVIESCMYTNTPDEQFILD--RHPKYDNIVIGAGFS 360

Query: 361 GHGFKMSPAVGRILAELALNGEAEGTELKYFKMARFEENPKGNV 401
           GHGFK++P VG+IL EL++       +L  F+++RF    K ++
Sbjct: 361 GHGFKLAPVVGKILYELSMK-LTPSYDLAPFRISRFPSLGKAHL 390

[Close]

BLAST of Lsi04G022000 vs. Swiss-Prot
Match: SOX_MOUSE (Peroxisomal sarcosine oxidase OS=Mus musculus GN=Pipox PE=1 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 4.4e-60
Identity = 140/404 (34.65%), Postives = 210/404 (51.98%), Query Frame = 1


Query: 1   MADSGTQFDVIVVGAGVMGSSTAYHLAKTGNRILILEQFDFLHHRGSSHGESRTIRATYP 60
           MA     +D IV+GAG+ G  TAYHLAK    +L+LEQF   H RGSSHG+SR IR  YP
Sbjct: 1   MAAQTDFWDAIVIGAGIQGCFTAYHLAKHSKSVLLLEQFFLPHSRGSSHGQSRIIRKAYP 60

Query: 61  EDYYHGLVMESYELWRMTEAEIGYRVYFPAEQLDIGPSDDKSLAAVVETCRKHSIPHLVL 120
           ED+Y  ++ E Y+ W   E E G +++   E L +G  ++  L  +  T  +  I H  L
Sbjct: 61  EDFYTMMMKECYQTWAQLEREAGTQLHRQTELLLLGTKENPGLKTIQATLSRQGIDHEYL 120

Query: 121 DRGQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQSLAYKNGAVLKDNAEVVEIK 180
               L +++   +      V +  K GGV+   KA+   Q +  + G  + D  +VVEI+
Sbjct: 121 SSVDLKQRFP-NIRFTRGEVGLLDKTGGVLYADKALRALQHIICQLGGTVCDGEKVVEIR 180

Query: 181 RDGSSGGIVVSTANGQSFRGKKCVVTVGAWARKLFKSVGGIELPIQPLEATVSYWRIKEG 240
                G  V      +S++    V+T G W  +L   + GIELP+Q L   V YWR K  
Sbjct: 181 ----PGLPVTVKTTLKSYQANSLVITAGPWTNRLLHPL-GIELPLQTLRINVCYWREK-- 240

Query: 241 VEAAEYAIGGDFPTF--ASYGDPYIYGTPSLEFPGLIKVAIHGGHQCDPDKRSWGN--GE 300
                Y +   FP          +IYG P+ E+PGL+K+  H G   DP++R       +
Sbjct: 241 -VPGSYGVSQAFPCILGLDLAPHHIYGLPASEYPGLMKICYHHGDNVDPEERDCPKTFSD 300

Query: 301 RLPIATLKEWIEERFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFS 360
              +  L  ++ +   G    +EP   + CMY+ TPDE F++D    +++ ++VIG GFS
Sbjct: 301 IQDVQILCHFVRDHLPGL--RAEPDIMERCMYTNTPDEHFILD-CHPKYD-NIVIGAGFS 360

Query: 361 GHGFKMSPAVGRILAELALNGEAEGTELKYFKMARFEENPKGNV 401
           GHGFK++P VG+IL EL++       +L  F+M+RF    K ++
Sbjct: 361 GHGFKLAPVVGKILYELSMK-LPPSYDLAPFRMSRFSTLSKAHL 390

[Close]

BLAST of Lsi04G022000 vs. TrEMBL
Match: A0A0A0LJG6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G881890 PE=4 SV=1)

HSP 1 Score: 743.0 bits (1917), Expect = 1.9e-211
Identity = 357/410 (87.07%), Postives = 380/410 (92.68%), Query Frame = 1


Query: 1   MADSGTQFDVIVVGAGVMGSSTAYHLAKTGNRILILEQFDFLHHRGSSHGESRTIRATYP 60
           MA S T FDVIVVGAGVMGSSTAYHLAKTGNR+LILEQFDFLHHRGSSHGESRTIRATYP
Sbjct: 1   MAASDTLFDVIVVGAGVMGSSTAYHLAKTGNRVLILEQFDFLHHRGSSHGESRTIRATYP 60

Query: 61  EDYYHGLVMESYELWRMTEAEIGYRVYFPAEQLDIGPSDDKSLAAVVETCRKHSIPHLVL 120
           EDYY+GLVMESYELWRM E EIGY+VYFP EQLDIG  DDKSL AVV+TCRKHSIPHLVL
Sbjct: 61  EDYYYGLVMESYELWRMAEEEIGYKVYFPTEQLDIGSPDDKSLTAVVDTCRKHSIPHLVL 120

Query: 121 DRGQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQSLAYKNGAVLKDNAEVVEIK 180
           D G+L EKYSGRVEIPADWV VWSKYGGVIKPTKAVSM+Q+LAYKNGAV+KDNAEVVEIK
Sbjct: 121 DSGELREKYSGRVEIPADWVGVWSKYGGVIKPTKAVSMYQTLAYKNGAVMKDNAEVVEIK 180

Query: 181 RDGSSGGIVVSTANGQSFRGKKCVVTVGAWARKLFKSVGGIELPIQPLEATVSYWRIKEG 240
           RD S+G IVVS ANG+SFRGKKCVVTVGAW++KL KSVGGIELPI+PLE +VSYWRIKEG
Sbjct: 181 RDESNGRIVVSIANGESFRGKKCVVTVGAWSKKLVKSVGGIELPIRPLEVSVSYWRIKEG 240

Query: 241 VEAAEYAIGGDFPTFASYGDPYIYGTPSLEFPGLIKVAIHGGHQCDPDKRSWGNGERLPI 300
            E AEYAIGG FPT ASYG+PY+YGTPSLEFPGLIKVAIHGGH+C+PDKRSWG G RLPI
Sbjct: 241 FE-AEYAIGGGFPTIASYGEPYVYGTPSLEFPGLIKVAIHGGHECNPDKRSWGKGGRLPI 300

Query: 301 ATLKEWIEERFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGF 360
           A LKEWI+E+FGGRVDSS PVSTQ CMYSMTPD DFVIDFLGGEFEKDVVIGGGFSGHGF
Sbjct: 301 AALKEWIDEKFGGRVDSSGPVSTQSCMYSMTPDGDFVIDFLGGEFEKDVVIGGGFSGHGF 360

Query: 361 KMSPAVGRILAELALNGEAEGTELKYFKMARFEENPKGNVKSFADQVKLH 411
           KMSP +GRILAELAL+G AEG ELKYFK+ARFEENPKGNVKSFADQVKLH
Sbjct: 361 KMSPTIGRILAELALDGAAEGVELKYFKLARFEENPKGNVKSFADQVKLH 409

[Close]

BLAST of Lsi04G022000 vs. TrEMBL
Match: A0A0D2SRF3_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_010G121600 PE=4 SV=1)

HSP 1 Score: 607.8 bits (1566), Expect = 9.5e-171
Identity = 290/409 (70.90%), Postives = 340/409 (83.13%), Query Frame = 1


Query: 1   MADSGTQFDVIVVGAGVMGSSTAYHLAKTGNRILILEQFDFLHHRGSSHGESRTIRATYP 60
           M  S  +FDVIVVGAGVMGSSTAY LAK G + L+LEQFDFLHHRGSSHGESRTIRATYP
Sbjct: 1   MGYSDNEFDVIVVGAGVMGSSTAYQLAKRGQKTLLLEQFDFLHHRGSSHGESRTIRATYP 60

Query: 61  EDYYHGLVMESYELWRMTEAEIGYRVYFPAEQLDIGPSDDKSLAAVVETCRKHSIPHLVL 120
           EDYY+GLV ESY LW   ++EIG++VYF A+Q D+GPSD KSL +V+ TCRK+ IP+ VL
Sbjct: 61  EDYYYGLVDESYRLWEQAQSEIGFKVYFKAQQFDMGPSDAKSLLSVISTCRKNGIPYQVL 120

Query: 121 DRGQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQSLAYKNGAVLKDNAEVVEIK 180
           D  Q+AE++SGR++IP DW+ V  + GG+IKPTKAVSMFQ LA+KNGA LKDN +VV I 
Sbjct: 121 DHRQVAERFSGRIDIPEDWIGVSCELGGIIKPTKAVSMFQMLAFKNGACLKDNIKVVSIN 180

Query: 181 RDGSSGGIVVSTANGQSFRGKKCVVTVGAWARKLFKSVGGIELPIQPLEATVSYWRIKEG 240
           +DG   G+ V+ +NG+ F GKKCVVTVG W R L K V GIELPIQPLE  V YWRIK+G
Sbjct: 181 KDGDR-GLKVAASNGEIFWGKKCVVTVGGWMRNLVKMVCGIELPIQPLETNVCYWRIKDG 240

Query: 241 VEAAEYAIGGDFPTFASYGDPYIYGTPSLEFPGLIKVAIHGGHQCDPDKRSWGNGERLPI 300
            E  EYAIG DFPTFASYG PYIYGTPSLE+PGLIKVA+HGG+QC+PDKR WG G  L  
Sbjct: 241 HE-VEYAIGNDFPTFASYGHPYIYGTPSLEYPGLIKVAVHGGYQCNPDKRPWGPG--LVP 300

Query: 301 ATLKEWIEERFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGF 360
            +LK+W+E+RF G+VDSS+P  TQLC+YSMTPDEDFVIDFLGGEF KDVVIGGGFSGHGF
Sbjct: 301 DSLKQWVEQRFKGKVDSSKPAMTQLCVYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGF 360

Query: 361 KMSPAVGRILAELALNGEAEGTELKYFKMARFEENPKGNVKSFADQVKL 410
           KMSPA+GRILA+LAL GEA+G ELK F++ARFEENP+GN+K + DQV+L
Sbjct: 361 KMSPAIGRILADLALIGEAKGVELKQFRIARFEENPRGNIKEYEDQVEL 405

[Close]

BLAST of Lsi04G022000 vs. TrEMBL
Match: M5XD12_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa006663mg PE=4 SV=1)

HSP 1 Score: 601.3 bits (1549), Expect = 8.9e-169
Identity = 288/403 (71.46%), Postives = 335/403 (83.13%), Query Frame = 1


Query: 1   MADSGTQFDVIVVGAGVMGSSTAYHLAKTGNRILILEQFDFLHHRGSSHGESRTIRATYP 60
           M  S  +FDVIVVGAG+MGSSTAY  AK G + L+LEQFDFLHHRGSSHGESRTIRATYP
Sbjct: 1   MEYSADEFDVIVVGAGIMGSSTAYQTAKRGQKTLLLEQFDFLHHRGSSHGESRTIRATYP 60

Query: 61  EDYYHGLVMESYELWRMTEAEIGYRVYFPAEQLDIGPSDDKSLAAVVETCRKHSIPHLVL 120
           EDYY  LV++SY+LW+  E+EIGY VYF A QLD+ P++DK L AVVE+CRK+ +P   +
Sbjct: 61  EDYYTPLVLQSYKLWQQAESEIGYNVYFKAHQLDMAPANDKVLHAVVESCRKNLVPFRFM 120

Query: 121 DRGQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQSLAYKNGAVLKDNAEVVEIK 180
           +R QL  ++SGR+ IP DWVAV +++GGVIKPTKAVSMFQ+LA +NGAVL+DN  V  ++
Sbjct: 121 NRDQLDREFSGRIRIPEDWVAVATEHGGVIKPTKAVSMFQTLALQNGAVLRDNMGVKGVE 180

Query: 181 RDGSSGGIVVSTANGQSFRGKKCVVTVGAWARKLFKSVGGIELPIQPLEATVSYWRIKEG 240
           RDG  GG+ V T NG+ F GKKCVVTVGAW  KL K+V GIELPI+PLE TV YWRIKEG
Sbjct: 181 RDGVRGGVWVCTENGERFWGKKCVVTVGAWTTKLVKTVAGIELPIKPLETTVCYWRIKEG 240

Query: 241 VEAAEYAIGGDFPTFASYGDPYIYGTPSLEFPGLIKVAIHGGHQCDPDKRSWGNGERLPI 300
            E   +AIGGDFPTFASYGD YIYGTPSLE+PGLIKVA+HGG+ CDPDKR WG G   P+
Sbjct: 241 HEGG-FAIGGDFPTFASYGDTYIYGTPSLEYPGLIKVAVHGGYPCDPDKRPWGPGN--PL 300

Query: 301 ATLKEWIEERFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGF 360
           A LKEWIE RF G VDS  PV+TQLCMYSMTPDEDFVIDFLGGEF KDVV+GGGFSGHGF
Sbjct: 301 APLKEWIEGRFSGVVDSGGPVATQLCMYSMTPDEDFVIDFLGGEFGKDVVVGGGFSGHGF 360

Query: 361 KMSPAVGRILAELALNGEAEGTELKYFKMARFEENPKGNVKSF 404
           K+SP VGRILA+LAL+GEA+G ELK+F++ARF+ENPKGNVK F
Sbjct: 361 KLSPVVGRILADLALSGEAQGVELKHFRIARFQENPKGNVKDF 400

[Close]

BLAST of Lsi04G022000 vs. TrEMBL
Match: A0A061GJC3_THECC (FAD-dependent oxidoreductase family protein OS=Theobroma cacao GN=TCM_036834 PE=4 SV=1)

HSP 1 Score: 598.2 bits (1541), Expect = 7.5e-168
Identity = 284/409 (69.44%), Postives = 336/409 (82.15%), Query Frame = 1


Query: 1   MADSGTQFDVIVVGAGVMGSSTAYHLAKTGNRILILEQFDFLHHRGSSHGESRTIRATYP 60
           M  S  +FDVIVVGAGVMGSSTAY LAK G + L+LEQFDFLHHRGSSHGESRTIRATYP
Sbjct: 1   MGYSADEFDVIVVGAGVMGSSTAYQLAKRGQKTLLLEQFDFLHHRGSSHGESRTIRATYP 60

Query: 61  EDYYHGLVMESYELWRMTEAEIGYRVYFPAEQLDIGPSDDKSLAAVVETCRKHSIPHLVL 120
           EDYYH +V ESY++W   ++EIG+RVYF A  +D+GP+D KSL AV+ TC++ S+PH VL
Sbjct: 61  EDYYHDMVNESYQMWEQAQSEIGFRVYFKARHVDMGPADAKSLLAVISTCQRKSMPHQVL 120

Query: 121 DRGQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQSLAYKNGAVLKDNAEVVEIK 180
           DR Q+ EK+SGR++IP  W+ V  ++GGVIKPTKAVSMFQ LA K+GA L DN EV  + 
Sbjct: 121 DRQQVTEKFSGRIDIPEGWIGVSCEHGGVIKPTKAVSMFQMLALKHGAFLWDNTEVNGVT 180

Query: 181 RDGSSGGIVVSTANGQSFRGKKCVVTVGAWARKLFKSVGGIELPIQPLEATVSYWRIKEG 240
           RDG  GG++VST+NG  F GKKCVVT G+W RKL K V G+ELPIQPLE  V YWRIKEG
Sbjct: 181 RDGVKGGVIVSTSNGDKFWGKKCVVTAGSWMRKLVKKVSGVELPIQPLETNVCYWRIKEG 240

Query: 241 VEAAEYAIGGDFPTFASYGDPYIYGTPSLEFPGLIKVAIHGGHQCDPDKRSWGNGERLPI 300
            E A+YAI  DFPTFASYG PY+YGTPSLE+PGLIKVA+HGG+ CDPDKR+WG G  +P 
Sbjct: 241 HE-AKYAIESDFPTFASYGKPYMYGTPSLEYPGLIKVAVHGGYPCDPDKRTWGPGV-IP- 300

Query: 301 ATLKEWIEERFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGF 360
           ++LK+WIEE F G VDSS P +TQLC+YSMTPDEDFV+DFLGGEF KDVVIGGGFSGHGF
Sbjct: 301 SSLKQWIEETFRGSVDSSGPAATQLCVYSMTPDEDFVLDFLGGEFGKDVVIGGGFSGHGF 360

Query: 361 KMSPAVGRILAELALNGEAEGTELKYFKMARFEENPKGNVKSFADQVKL 410
           KM+P +GRILA+L L G+AEG ELK+F++ARF+E+P GNVK F DQV L
Sbjct: 361 KMAPVIGRILADLVLTGKAEGIELKHFRIARFKEHPGGNVKDFEDQVGL 406

[Close]

BLAST of Lsi04G022000 vs. TrEMBL
Match: A0A067FIU9_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g015088mg PE=4 SV=1)

HSP 1 Score: 595.5 bits (1534), Expect = 4.9e-167
Identity = 280/405 (69.14%), Postives = 339/405 (83.70%), Query Frame = 1


Query: 5   GTQFDVIVVGAGVMGSSTAYHLAKTGNRILILEQFDFLHHRGSSHGESRTIRATYPEDYY 64
           G +FDVIVVGAG+MGSS AY LAK G + L+LEQFDFLHHRGSSHGESRTIRATYPEDYY
Sbjct: 5   GEKFDVIVVGAGIMGSSAAYQLAKRGQKTLLLEQFDFLHHRGSSHGESRTIRATYPEDYY 64

Query: 65  HGLVMESYELWRMTEAEIGYRVYFPAEQLDIGPSDDKSLAAVVETCRKHSIPHLVLDRGQ 124
           H +V+ES  LW   ++EIGY+VYF A Q D+GPS++KSL +V+ +CRK+S+PH VLD  Q
Sbjct: 65  HPMVLESCLLWEQAQSEIGYKVYFKAHQFDMGPSENKSLRSVIASCRKNSVPHQVLDCRQ 124

Query: 125 LAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQSLAYKNGAVLKDNAEVVEI--KRD 184
           + EKYSGR+EIP +WV V ++ GGVIKPTKAVSMFQ+LA KNGAVL+DN EV  +   +D
Sbjct: 125 VLEKYSGRIEIPENWVGVATELGGVIKPTKAVSMFQTLAIKNGAVLRDNMEVKTVLKVKD 184

Query: 185 GSSGGIVVSTANGQSFRGKKCVVTVGAWARKLFKSVGGIELPIQPLEATVSYWRIKEGVE 244
              GG+ V T+NG+ F GKKCVVT GAW  KL K + G+ELPIQ +E TV YWRIKEG E
Sbjct: 185 AVKGGVTVVTSNGEKFWGKKCVVTAGAWVGKLVKRITGLELPIQAVETTVCYWRIKEGNE 244

Query: 245 AAEYAIGGDFPTFASYGDPYIYGTPSLEFPGLIKVAIHGGHQCDPDKRSWGNGERLPIAT 304
            A+YA+GGDFP+FASYGDPYIYGTPSLE+PGLIK+A+HGG+ CDPD+R WG G  L + +
Sbjct: 245 -ADYAVGGDFPSFASYGDPYIYGTPSLEYPGLIKIALHGGYPCDPDRRPWGPG--LLLDS 304

Query: 305 LKEWIEERFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGFKM 364
           LKEWI+ RF GRVDS+ PV+TQLCMYS+TPDEDFVIDFLGGEF +DVV+ GGFSGHGFKM
Sbjct: 305 LKEWIQGRFAGRVDSNGPVATQLCMYSITPDEDFVIDFLGGEFGEDVVVAGGFSGHGFKM 364

Query: 365 SPAVGRILAELALNGEAEGTELKYFKMARFEENPKGNVKSFADQV 408
           +PAVGRILA+L L+GEA+G EL++F+++RF+ENPKGNVK + DQV
Sbjct: 365 APAVGRILADLVLSGEAQGVELQHFRISRFKENPKGNVKDYEDQV 406

[Close]

BLAST of Lsi04G022000 vs. TAIR10
Match: AT2G24580.1 (AT2G24580.1 FAD-dependent oxidoreductase family protein)

HSP 1 Score: 569.3 bits (1466), Expect = 1.9e-162
Identity = 269/411 (65.45%), Postives = 328/411 (79.81%), Query Frame = 1


Query: 2   ADSGTQFDVIVVGAGVMGSSTAYHLAKTGNRILILEQFDFLHHRGSSHGESRTIRATYPE 61
           +D G +FDVIVVGAGVMGSS AY LAK G + L+LEQFDFLHHRGSSHGESRTIRATYPE
Sbjct: 4   SDDG-RFDVIVVGAGVMGSSAAYQLAKRGQKTLLLEQFDFLHHRGSSHGESRTIRATYPE 63

Query: 62  DYYHGLVMESYELWRMTEAEIGYRVYFPAEQLDIGPSDDKSLAAVVETCRKHSIPHLVLD 121
           DYY+ +V ES  LW   ++EIGY+V+FP +Q D+GP+D +SL +VV TC+KH + H V+D
Sbjct: 64  DYYYSMVSESTRLWAAAQSEIGYKVHFPTQQFDMGPADQQSLLSVVATCQKHGLAHRVMD 123

Query: 122 RGQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQSLAYKNGAVLKDNAEVVEIKR 181
              ++E +SGR+ IP +W+ V ++ GG+IKPTKAVSMFQ+LA  +GA+L+DN +V  IKR
Sbjct: 124 SHAVSEHFSGRISIPENWIGVSTELGGIIKPTKAVSMFQTLAIGHGAILRDNTKVANIKR 183

Query: 182 DGSSG-GIVVSTANGQSFRGKKCVVTVGAWARKLFKSVGGIELPIQPLEATVSYWRIKEG 241
           DG SG G++V T  G  F GKKC+VT GAW  KL K+V GI+ P++PLE TV YWRIKEG
Sbjct: 184 DGESGEGVIVCTVKGDKFYGKKCIVTAGAWISKLVKTVAGIDFPVEPLETTVCYWRIKEG 243

Query: 242 VEAAEYAIGGDFPTFASYGDPYIYGTPSLEFPGLIKVAIHGGHQCDPDKRSWGNGERLPI 301
            E  ++ I G+FPTFASYG PY+YGTPSLE+PGLIKVA+HGG+ CDPDKR WG G +L  
Sbjct: 244 HE-EKFTIDGEFPTFASYGAPYVYGTPSLEYPGLIKVAVHGGYWCDPDKRPWGPGVKL-- 303

Query: 302 ATLKEWIEERFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGF 361
             LKEWI+ERFGG VDS  PV+TQLCMYSMTPDEDFVIDFLGGEF +DVV+GGGFSGHGF
Sbjct: 304 EELKEWIKERFGGMVDSEGPVATQLCMYSMTPDEDFVIDFLGGEFGRDVVVGGGFSGHGF 363

Query: 362 KMSPAVGRILAELALNGEA--EGTELKYFKMARFEENPKGNVKSFADQVKL 410
           KM+PAVGRILA++A+  EA   G E+K F + RFE+NPKGN K + DQV L
Sbjct: 364 KMAPAVGRILADMAMEVEAGGGGVEMKQFSLRRFEDNPKGNAKEYPDQVIL 410

[Close]

BLAST of Lsi04G022000 vs. NCBI nr
Match: gi|449437334|ref|XP_004136447.1| (PREDICTED: probable sarcosine oxidase [Cucumis sativus])

HSP 1 Score: 743.0 bits (1917), Expect = 2.7e-211
Identity = 357/410 (87.07%), Postives = 380/410 (92.68%), Query Frame = 1


Query: 1   MADSGTQFDVIVVGAGVMGSSTAYHLAKTGNRILILEQFDFLHHRGSSHGESRTIRATYP 60
           MA S T FDVIVVGAGVMGSSTAYHLAKTGNR+LILEQFDFLHHRGSSHGESRTIRATYP
Sbjct: 1   MAASDTLFDVIVVGAGVMGSSTAYHLAKTGNRVLILEQFDFLHHRGSSHGESRTIRATYP 60

Query: 61  EDYYHGLVMESYELWRMTEAEIGYRVYFPAEQLDIGPSDDKSLAAVVETCRKHSIPHLVL 120
           EDYY+GLVMESYELWRM E EIGY+VYFP EQLDIG  DDKSL AVV+TCRKHSIPHLVL
Sbjct: 61  EDYYYGLVMESYELWRMAEEEIGYKVYFPTEQLDIGSPDDKSLTAVVDTCRKHSIPHLVL 120

Query: 121 DRGQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQSLAYKNGAVLKDNAEVVEIK 180
           D G+L EKYSGRVEIPADWV VWSKYGGVIKPTKAVSM+Q+LAYKNGAV+KDNAEVVEIK
Sbjct: 121 DSGELREKYSGRVEIPADWVGVWSKYGGVIKPTKAVSMYQTLAYKNGAVMKDNAEVVEIK 180

Query: 181 RDGSSGGIVVSTANGQSFRGKKCVVTVGAWARKLFKSVGGIELPIQPLEATVSYWRIKEG 240
           RD S+G IVVS ANG+SFRGKKCVVTVGAW++KL KSVGGIELPI+PLE +VSYWRIKEG
Sbjct: 181 RDESNGRIVVSIANGESFRGKKCVVTVGAWSKKLVKSVGGIELPIRPLEVSVSYWRIKEG 240

Query: 241 VEAAEYAIGGDFPTFASYGDPYIYGTPSLEFPGLIKVAIHGGHQCDPDKRSWGNGERLPI 300
            E AEYAIGG FPT ASYG+PY+YGTPSLEFPGLIKVAIHGGH+C+PDKRSWG G RLPI
Sbjct: 241 FE-AEYAIGGGFPTIASYGEPYVYGTPSLEFPGLIKVAIHGGHECNPDKRSWGKGGRLPI 300

Query: 301 ATLKEWIEERFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGF 360
           A LKEWI+E+FGGRVDSS PVSTQ CMYSMTPD DFVIDFLGGEFEKDVVIGGGFSGHGF
Sbjct: 301 AALKEWIDEKFGGRVDSSGPVSTQSCMYSMTPDGDFVIDFLGGEFEKDVVIGGGFSGHGF 360

Query: 361 KMSPAVGRILAELALNGEAEGTELKYFKMARFEENPKGNVKSFADQVKLH 411
           KMSP +GRILAELAL+G AEG ELKYFK+ARFEENPKGNVKSFADQVKLH
Sbjct: 361 KMSPTIGRILAELALDGAAEGVELKYFKLARFEENPKGNVKSFADQVKLH 409

[Close]

BLAST of Lsi04G022000 vs. NCBI nr
Match: gi|659132624|ref|XP_008466296.1| (PREDICTED: probable sarcosine oxidase [Cucumis melo])

HSP 1 Score: 732.3 bits (1889), Expect = 4.8e-208
Identity = 354/410 (86.34%), Postives = 379/410 (92.44%), Query Frame = 1


Query: 1   MADSGTQFDVIVVGAGVMGSSTAYHLAKTGNRILILEQFDFLHHRGSSHGESRTIRATYP 60
           MA S TQFDVIVVGAGVMGSSTAYHLAKTGNR+L+LEQFDFLHHRGSSHGESRTIRATYP
Sbjct: 1   MAASDTQFDVIVVGAGVMGSSTAYHLAKTGNRVLLLEQFDFLHHRGSSHGESRTIRATYP 60

Query: 61  EDYYHGLVMESYELWRMTEAEIGYRVYFPAEQLDIGPSDDKSLAAVVETCRKHSIPHLVL 120
           EDYYH LVMESYELWRM EAEIG++VY+PAEQLDIGPS+ +SL AVV+TC KHSIPHLVL
Sbjct: 61  EDYYHDLVMESYELWRMAEAEIGFKVYYPAEQLDIGPSNSESLTAVVDTCLKHSIPHLVL 120

Query: 121 DRGQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQSLAYKNGAVLKDNAEVVEIK 180
           DRG+L EKYSGRVEIPA+WVAV SKYGGVIKPTKAVSMFQ+LAYKNG VLKDNAEVVEIK
Sbjct: 121 DRGELMEKYSGRVEIPANWVAVCSKYGGVIKPTKAVSMFQTLAYKNGTVLKDNAEVVEIK 180

Query: 181 RDGSSGGIVVSTANGQSFRGKKCVVTVGAWARKLFKSVGGIELPIQPLEATVSYWRIKEG 240
           RD S+G IVVSTANG+SF GKKCVVTVGAW++KL KSVGGIELPI PLE +VSYWRIKEG
Sbjct: 181 RDESNGRIVVSTANGESFHGKKCVVTVGAWSKKLVKSVGGIELPILPLEVSVSYWRIKEG 240

Query: 241 VEAAEYAIGGDFPTFASYGDPYIYGTPSLEFPGLIKVAIHGGHQCDPDKRSWGNGERLPI 300
            E AEYAI G FPT ASYG+PY+YGTPSLEFPGLIKVAIH G+ C+PDKRSWG   RLPI
Sbjct: 241 FE-AEYAIEGGFPTMASYGEPYVYGTPSLEFPGLIKVAIHAGYSCNPDKRSWGREGRLPI 300

Query: 301 ATLKEWIEERFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGF 360
           A LKEWI+E+FGGRVDSS PVS+QLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGF
Sbjct: 301 AALKEWIDEKFGGRVDSSRPVSSQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGF 360

Query: 361 KMSPAVGRILAELALNGEAEGTELKYFKMARFEENPKGNVKSFADQVKLH 411
           KMSPA+GRILAELAL G AEG ELKYFK+ARFEENPKGNVKSFADQVKLH
Sbjct: 361 KMSPAIGRILAELALKGAAEGVELKYFKLARFEENPKGNVKSFADQVKLH 409

[Close]

BLAST of Lsi04G022000 vs. NCBI nr
Match: gi|1009156308|ref|XP_015896180.1| (PREDICTED: probable sarcosine oxidase [Ziziphus jujuba])

HSP 1 Score: 619.8 bits (1597), Expect = 3.5e-174
Identity = 288/404 (71.29%), Postives = 343/404 (84.90%), Query Frame = 1


Query: 4   SGTQFDVIVVGAGVMGSSTAYHLAKTGNRILILEQFDFLHHRGSSHGESRTIRATYPEDY 63
           SG  FDVIVVGAG+MGSSTAY  +K G++ L+LEQFDFLHHRGSSHGESRTIR TYP+DY
Sbjct: 5   SGEDFDVIVVGAGIMGSSTAYQTSKRGHKTLLLEQFDFLHHRGSSHGESRTIRPTYPQDY 64

Query: 64  YHGLVMESYELWRMTEAEIGYRVYFPAEQLDIGPSDDKSLAAVVETCRKHSIPHLVLDRG 123
           Y  +V+ESY LW   E+EIGY+V F A Q D+GP+D  +  A++ +CRK+S+PH VLD+ 
Sbjct: 65  YCSMVLESYTLWEQAESEIGYKVSFKASQFDMGPADATNFKALISSCRKNSLPHQVLDKA 124

Query: 124 QLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQSLAYKNGAVLKDNAEVVEIKRDG 183
           Q+A+K+SGR+ IP DWV V ++YGGVIKPTKAVSMFQ+LA KNGAVL+DN EV +IKRDG
Sbjct: 125 QVAQKFSGRIHIPEDWVGVSTEYGGVIKPTKAVSMFQTLALKNGAVLRDNMEVKDIKRDG 184

Query: 184 SSGGIVVSTANGQSFRGKKCVVTVGAWARKLFKSVGGIELPIQPLEATVSYWRIKEGVEA 243
             GG+VV T+NG+ FRGKKCVVTVGAW +KL K+V G+E+PIQPLE TV YWRI EG E 
Sbjct: 185 EGGGLVVFTSNGEKFRGKKCVVTVGAWMKKLVKTVIGVEIPIQPLETTVCYWRINEGHE- 244

Query: 244 AEYAIGGDFPTFASYGDPYIYGTPSLEFPGLIKVAIHGGHQCDPDKRSWGNGERLPIATL 303
            +YAIGGDFPTFASYG+PY+YGTPSLEFPGLIKVA+HGG+ CDPDKR WG G  + +A L
Sbjct: 245 TDYAIGGDFPTFASYGEPYVYGTPSLEFPGLIKVAVHGGYACDPDKRPWGPG--ISLAAL 304

Query: 304 KEWIEERFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGFKMS 363
           KEWI+ RF G VDS+ PV+TQLCMYSMTPDEDFV+DFLGGEF KD+VIGGGFSGHGFKM+
Sbjct: 305 KEWIQGRFSGLVDSAGPVATQLCMYSMTPDEDFVMDFLGGEFGKDLVIGGGFSGHGFKMA 364

Query: 364 PAVGRILAELALNGEAEGTELKYFKMARFEENPKGNVKSFADQV 408
           P VGRILA+L L+GEAEG +LK+F++ARF ENPKGNVK+F DQV
Sbjct: 365 PVVGRILADLVLSGEAEGVDLKHFRVARFNENPKGNVKAFEDQV 405

[Close]

BLAST of Lsi04G022000 vs. NCBI nr
Match: gi|1009172411|ref|XP_015867257.1| (PREDICTED: probable sarcosine oxidase [Ziziphus jujuba])

HSP 1 Score: 617.1 bits (1590), Expect = 2.3e-173
Identity = 287/404 (71.04%), Postives = 342/404 (84.65%), Query Frame = 1


Query: 4   SGTQFDVIVVGAGVMGSSTAYHLAKTGNRILILEQFDFLHHRGSSHGESRTIRATYPEDY 63
           SG  FDVIVVGAG+MGSSTAY  +K G++ L+LEQFDFLHHRGSSHGESRTIR TYP+DY
Sbjct: 5   SGEDFDVIVVGAGIMGSSTAYQTSKRGHKTLLLEQFDFLHHRGSSHGESRTIRPTYPQDY 64

Query: 64  YHGLVMESYELWRMTEAEIGYRVYFPAEQLDIGPSDDKSLAAVVETCRKHSIPHLVLDRG 123
           Y  +V+ESY LW   E+EIGY+V F A Q D+GP+D  +  A++ +CRK+S+PH VLD+ 
Sbjct: 65  YCSMVLESYTLWEQAESEIGYKVSFKASQFDMGPADATNFKALISSCRKNSLPHQVLDKA 124

Query: 124 QLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQSLAYKNGAVLKDNAEVVEIKRDG 183
           Q+A+K+SGR+ IP DWV V ++YGG IKPTKAVSMFQ+LA KNGAVL+DN EV +IKRDG
Sbjct: 125 QVAQKFSGRIHIPEDWVGVSTEYGGGIKPTKAVSMFQTLALKNGAVLRDNMEVKDIKRDG 184

Query: 184 SSGGIVVSTANGQSFRGKKCVVTVGAWARKLFKSVGGIELPIQPLEATVSYWRIKEGVEA 243
             GG+VV T+NG+ FRGKKCVVTVGAW +KL K+V G+E+PIQPLE TV YWRI EG E 
Sbjct: 185 EGGGLVVFTSNGEKFRGKKCVVTVGAWMKKLVKTVIGVEIPIQPLETTVCYWRINEGHE- 244

Query: 244 AEYAIGGDFPTFASYGDPYIYGTPSLEFPGLIKVAIHGGHQCDPDKRSWGNGERLPIATL 303
            +YAIGGDFPTFASYG+PY+YGTPSLEFPGLIKVA+HGG+ CDPDKR WG G  + +A L
Sbjct: 245 TDYAIGGDFPTFASYGEPYVYGTPSLEFPGLIKVAVHGGYACDPDKRPWGPG--ISLAAL 304

Query: 304 KEWIEERFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGFKMS 363
           KEWI+ RF G VDS+ PV+TQLCMYSMTPDEDFV+DFLGGEF KD+VIGGGFSGHGFKM+
Sbjct: 305 KEWIQGRFSGLVDSAGPVATQLCMYSMTPDEDFVMDFLGGEFGKDLVIGGGFSGHGFKMA 364

Query: 364 PAVGRILAELALNGEAEGTELKYFKMARFEENPKGNVKSFADQV 408
           P VGRILA+L L+GEAEG +LK+F++ARF ENPKGNVK+F DQV
Sbjct: 365 PVVGRILADLVLSGEAEGVDLKHFRVARFNENPKGNVKAFEDQV 405

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BLAST of Lsi04G022000 vs. NCBI nr
Match: gi|694324599|ref|XP_009353308.1| (PREDICTED: probable sarcosine oxidase [Pyrus x bretschneideri])

HSP 1 Score: 612.1 bits (1577), Expect = 7.2e-172
Identity = 297/403 (73.70%), Postives = 339/403 (84.12%), Query Frame = 1


Query: 1   MADSGTQFDVIVVGAGVMGSSTAYHLAKTGNRILILEQFDFLHHRGSSHGESRTIRATYP 60
           M  SG +FDVIVVGAGVMGSSTAY  AK G++ L+LEQFDFLHHRGSSHGESRTIRATYP
Sbjct: 1   MEYSGDEFDVIVVGAGVMGSSTAYQTAKRGHKTLLLEQFDFLHHRGSSHGESRTIRATYP 60

Query: 61  EDYYHGLVMESYELWRMTEAEIGYRVYFPAEQLDIGPSDDKSLAAVVETCRKHSIPHLVL 120
           EDYY  LV+ESY+LW+  E+EIGY VYF A QLD+  ++DK L AVVE+CRK+S+   V+
Sbjct: 61  EDYYTPLVLESYKLWQQAESEIGYNVYFKATQLDMALANDKLLLAVVESCRKNSVAFSVM 120

Query: 121 DRGQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQSLAYKNGAVLKDNAEVVEIK 180
           +R QL +++SGRV IP DWV V +++GGVIKPTKAVSMFQ+LA +NGAVL+DN EV  ++
Sbjct: 121 NRDQLHQEFSGRVMIPEDWVGVVTEHGGVIKPTKAVSMFQTLALQNGAVLRDNMEVKGVE 180

Query: 181 RDGSSGGIVVSTANGQSFRGKKCVVTVGAWARKLFKSVGGIELPIQPLEATVSYWRIKEG 240
           RDG  GG+ VSTA G+ F GKKCVVTVGAW  KL K+VGGIELPIQPLE TV YWRIKEG
Sbjct: 181 RDGVRGGVWVSTAKGERFWGKKCVVTVGAWTTKLVKTVGGIELPIQPLETTVCYWRIKEG 240

Query: 241 VEAAEYAIGGDFPTFASYGDPYIYGTPSLEFPGLIKVAIHGGHQCDPDKRSWGNGERLPI 300
            E A +AIGGDFPTFASYG+PYIYGTPSLE+PGLIKVA+HGG+ CDPDKR WG G   P+
Sbjct: 241 HEGA-FAIGGDFPTFASYGNPYIYGTPSLEYPGLIKVAVHGGYPCDPDKRPWGPGN--PL 300

Query: 301 ATLKEWIEERFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGF 360
           A LKEWIE  F G VDS  PV+TQLCMYSMTPDEDFVIDFLGGEF KDVV+GGGFSGHGF
Sbjct: 301 APLKEWIEGMFSGVVDSGGPVATQLCMYSMTPDEDFVIDFLGGEFGKDVVVGGGFSGHGF 360

Query: 361 KMSPAVGRILAELALNGEAEGTELKYFKMARFEENPKGNVKSF 404
           KMSP VGRILA+LAL GEAEG ELK+F+MARF+ENPKGN K F
Sbjct: 361 KMSPVVGRILADLALTGEAEGVELKHFRMARFQENPKGNAKDF 400

The following BLAST results are available for this feature:

BLAST of Lsi04G022000 vs. Swiss-Prot


Match Name	E-value	Identity	Description
SOX_ARATH	3.4e-161	65.45	Probable sarcosine oxidase OS=Arabidopsis thaliana GN=At2g24580 PE=2 SV=1	[more]
SOX_BOVIN	1.6e-62	34.98	Peroxisomal sarcosine oxidase OS=Bos taurus GN=PIPOX PE=2 SV=2	[more]
SOX_HUMAN	1.0e-61	34.41	Peroxisomal sarcosine oxidase OS=Homo sapiens GN=PIPOX PE=1 SV=2	[more]
SOX_RABIT	8.8e-61	33.91	Peroxisomal sarcosine oxidase OS=Oryctolagus cuniculus GN=PIPOX PE=1 SV=1	[more]
SOX_MOUSE	4.4e-60	34.65	Peroxisomal sarcosine oxidase OS=Mus musculus GN=Pipox PE=1 SV=1	[more]

BLAST of Lsi04G022000 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LJG6_CUCSA	1.9e-211	87.07	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G881890 PE=4 SV=1	[more]
A0A0D2SRF3_GOSRA	9.5e-171	70.90	Uncharacterized protein OS=Gossypium raimondii GN=B456_010G121600 PE=4 SV=1	[more]
M5XD12_PRUPE	8.9e-169	71.46	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa006663mg PE=4 SV=1	[more]
A0A061GJC3_THECC	7.5e-168	69.44	FAD-dependent oxidoreductase family protein OS=Theobroma cacao GN=TCM_036834 PE=...	[more]
A0A067FIU9_CITSI	4.9e-167	69.14	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g015088mg PE=4 SV=1	[more]

BLAST of Lsi04G022000 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G24580.1	1.9e-162	65.45	FAD-dependent oxidoreductase family protein	[more]

BLAST of Lsi04G022000 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449437334\|ref\|XP_004136447.1\|	2.7e-211	87.07	PREDICTED: probable sarcosine oxidase [Cucumis sativus]	[more]
gi\|659132624\|ref\|XP_008466296.1\|	4.8e-208	86.34	PREDICTED: probable sarcosine oxidase [Cucumis melo]	[more]
gi\|1009156308\|ref\|XP_015896180.1\|	3.5e-174	71.29	PREDICTED: probable sarcosine oxidase [Ziziphus jujuba]	[more]
gi\|1009172411\|ref\|XP_015867257.1\|	2.3e-173	71.04	PREDICTED: probable sarcosine oxidase [Ziziphus jujuba]	[more]
gi\|694324599\|ref\|XP_009353308.1\|	7.2e-172	73.70	PREDICTED: probable sarcosine oxidase [Pyrus x bretschneideri]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0046653	tetrahydrofolate metabolic process
GO:0055114	oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0008115	sarcosine oxidase activity
GO:0016491	oxidoreductase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR023753	FAD/NAD-binding_dom
IPR006281	SoxA_mon
IPR006076	FAD-dep_OxRdtase

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006544	glycine metabolic process
biological_process	GO:0006563	L-serine metabolic process
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0046653	tetrahydrofolate metabolic process
biological_process	GO:0006566	threonine metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0008115	sarcosine oxidase activity
molecular_function	GO:0016491	oxidoreductase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G022000.1	Lsi04G022000.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006076	FAD dependent oxidoreductase	PFAM	PF01266	DAO	coord: 9..373 score: 4.7
IPR006281	Sarcosine oxidase, monomeric	TIGRFAMs	TIGR01377	TIGR01377	coord: 8..398 score: 6.4E
IPR023753	FAD/NAD(P)-binding domain	GENE3D	G3DSA:3.50.50.60		coord: 152..216 score: 1.5E-56coord: 7..62 score: 1.5E-56coord: 335..396 score: 1.5
IPR023753	FAD/NAD(P)-binding domain	unknown	SSF51905	FAD/NAD(P)-binding domain	coord: 332..393 score: 1.88E-45coord: 7..225 score: 1.88
None	No IPR available	GENE3D	G3DSA:3.30.9.10		coord: 63..151 score: 9.1E-38coord: 223..332 score: 9.1
None	No IPR available	PANTHER	PTHR10961	PEROXISOMAL SARCOSINE OXIDASE	coord: 1..407 score: 4.2E
None	No IPR available	PANTHER	PTHR10961:SF7	PEROXISOMAL SARCOSINE OXIDASE	coord: 1..407 score: 4.2E
None	No IPR available	unknown	SSF54373	FAD-linked reductases, C-terminal domain	coord: 226..332 score: 2.08

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi04G022000	Csa3G881890	Cucumber (Chinese Long) v2	culsiB191
Lsi04G022000	CSPI03G44710	Wild cucumber (PI 183967)	cpilsiB189
Lsi04G022000	Cucsa.311080	Cucumber (Gy14) v1	cgylsiB503
Lsi04G022000	CmaCh09G005320	Cucurbita maxima (Rimu)	cmalsiB022
Lsi04G022000	CmoCh09G005220	Cucurbita moschata (Rifu)	cmolsiB010
Lsi04G022000	Cla009971	Watermelon (97103) v1	lsiwmB321
Lsi04G022000	MELO3C003620	Melon (DHL92) v3.5.1	lsimeB310
Lsi04G022000	ClCG05G026450	Watermelon (Charleston Gray)	lsiwcgB292
Lsi04G022000	MELO3C003620.2	Melon (DHL92) v3.6.1	lsimedB324
Lsi04G022000	Cla97C05G108680	Watermelon (97103) v2	lsiwmbB293
Lsi04G022000	Bhi09G002016	Wax gourd	lsiwgoB393
Lsi04G022000	Cp4.1LG06g04570	Cucurbita pepo (Zucchini)	cpelsiB636
Lsi04G022000	CsGy3G042150	Cucumber (Gy14) v2	cgyblsiB175
Lsi04G022000	Carg02942	Silver-seed gourd	carlsiB575
Lsi04G022000	CsaV3_3G047160	Cucumber (Chinese Long) v3	cuclsiB208

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi04G022000	Melon (DHL92) v3.6.1	lsimedB312
Lsi04G022000	Silver-seed gourd	carlsiB055
Lsi04G022000	Silver-seed gourd	carlsiB499
Lsi04G022000	Silver-seed gourd	carlsiB897
Lsi04G022000	Cucumber (Chinese Long) v3	cuclsiB136
Lsi04G022000	Watermelon (97103) v2	lsiwmbB308
Lsi04G022000	Wax gourd	lsiwgoB358
Lsi04G022000	Bottle gourd (USVL1VR-Ls)	lsilsiB153
Lsi04G022000	Cucumber (Gy14) v1	cgylsiB264
Lsi04G022000	Cucurbita maxima (Rimu)	cmalsiB176
Lsi04G022000	Cucurbita maxima (Rimu)	cmalsiB425
Lsi04G022000	Cucurbita maxima (Rimu)	cmalsiB730
Lsi04G022000	Cucurbita moschata (Rifu)	cmolsiB164
Lsi04G022000	Cucurbita moschata (Rifu)	cmolsiB413
Lsi04G022000	Cucurbita moschata (Rifu)	cmolsiB721
Lsi04G022000	Cucurbita pepo (Zucchini)	cpelsiB092
Lsi04G022000	Cucurbita pepo (Zucchini)	cpelsiB463
Lsi04G022000	Wild cucumber (PI 183967)	cpilsiB120
Lsi04G022000	Cucumber (Chinese Long) v2	culsiB123
Lsi04G022000	Melon (DHL92) v3.5.1	lsimeB298
Lsi04G022000	Watermelon (Charleston Gray)	lsiwcgB306
Lsi04G022000	Watermelon (97103) v1	lsiwmB294
Lsi04G022000	Cucumber (Gy14) v2	cgyblsiB110