Lsi04G019600 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi04G019600
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionCytochrome P450 family 72 protein
Locationchr04 : 26698375 .. 26704601 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATAGTATTTAATTAAATGTAATTTTATTCAGAAACATTAATGCATGTGTTTTTGGAGTGCAGGCAAGTATTCATTCATGTGGCTTGGACCCATACCAAGAGTGCATATCATGGACCCTGAACAACTTAAAATTGTGTTTTCATTGATCAATGATTTCCAAAAGCCAACTATAAACCCCCTTGTCAGGTTGCTTGCAAATGGACTTGCAAATCATGAAGGACCAAAATGGGTCAAACACAGAAAGATAATCAACCATGCATTTCATTTACAAAAGCTTAAGGTATCTCTTTTTCTCCATATCACTTTTAAACTATATTGAAAACTTTCACAAACAAATAATATGCTAATATTCTATCATAATCTTATCAACATCATCAAAAAATAATCTTATCAACCATGCATTTCTATTTCATCTTCCTATTCGGTAACTATTTTTATTTATTGTTCTTGTTTCTAGTTCATAAGAAATAGGAAAAAGAAAACTGAAACGTTATCAAACAATACATATAATTTCAAGATCATTTACATCATACTTTATCGTAAAATTTGTTGAAATGTAATCAAATGTCATACATACCGTTACATTGGCTTATAGGATATGGTACCAGCATTTTATCACAGCTGTAACGAGATGATTAGTAAATGGGAAAGCATGGTCTTTGTAGAAGGATCTTGTGAGTTAGATGTAATGCCTTATCTACAAAATATGGCTGCTGATGTGATTTCTCGAACTGCATTTGGGAGTAGCTATTAGAAAGGAAAAAAGATATTCAAGCTTCAAGCTGAACTAGCTGATTTGGCGATTCAATCAACCTTAGGGATTTTTATATTCCAGGATGGAGGTAAAACCTTAATCCATGCATGTACAACATCATTATAAACATCTATTGTGAAAGATATGATATCATGATTAATTTTGTGCAGATTTCTACCCACAAAGTCAAACAATAAAATGAAAGAGATAAGTAAAGAGATAACAACTTTGATCTTGGGTATTATGAATGAAAGGGAAAAGTCAATGAAGGCAGGTGAAGTTATACAAACTGATCTACTAAGCATTCTCATGGAATCCAATTTGAATGAAATTAAACAACATGGAAACAATAAAGACATTGGAATAAGCATAGAAGATGTTATTGCAGAGTGCAAGCTTTTCTATATTGCTGGCCAAGAAACTACAGCTACGTTACTAGTTTGGACGATGGTATTGTTGAGTTCATACTCAGAATGGCAAGAACAAGCAAGAGCAGAGGTCTTTGAGATCTTTGGTAACAAGAAACCAGATTATGATGGTTTAAGTCGACTAAAAGTTGTGAGTGTGATGATATTTTCTTTGAATTTACTTTATGTTTATCTATTTTTCTCATTTCAGTTTTATCTCATATATGAAATTAAATGCCCATCTTTCGATATAAAGCAATCATATGAGAAAGTTTTGGAGGAATCAATACTGAATAGTCTAGAAAGGTTTCTAACCTTAATCTCAATCAAGCTTATTGCCTTTGGACTATGTAAGTTGAAAGGATAAAGGCTATTTGATAAAATTGGTATCTAAGCATGAACACTTTAGTTTGACAAGTGTGCCAATGTGTGTTGGACACTTGGACACTCTGGCATGTATCGAACACTTGTTAGTGCAATATATGTGTTAGAAACTAGTTGTACAAAATCAAATAGATCATCCAATATTTGTTAGATATGCATCGAACACTTGATGAGTATATTAAATAGACACGCATGACAAAAATAATAAATTTTGATAGTGAAATACATCAAACTAATTTCTTAAGCATATAAATGCATAAACTCATTGACTTTAAATTTCTTTCTTGTATAATAATGATATATATATATATATATATATATATATATATATATATATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN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mRNA sequence

ATGGATAGCAAGTATTCATTCATGTGGCTTGGACCCATACCAAGAGTGCATATCATGGACCCTGAACAACTTAAAATTGTGTTTTCATTGATCAATGATTTCCAAAAGCCAACTATAAACCCCCTTGTCAGGTTGCTTGCAAATGGACTTGCAAATCATGAAGGACCAAAATGGGTCAAACACAGAAAGATAATCAACCATGCATTTCATTTACAAAAGCTTAAGGATATGGTACCAGCATTTTATCACAGCTGTAACGAGATGATTAGTAAATGGGAAAGCATGGTCTTTGTAGAAGGATCTTGTGAGTTAGATGTAATGCCTTATCTACAAAATATGGCTGCTGATGTGATTTCTCGAACTGCATTTGGGAGATGGAGATTTCTACCCACAAAGTCAAACAATAAAATGAAAGAGATAAGTAAAGAGATAACAACTTTGATCTTGGGTATTATGAATGAAAGGGAAAAGTCAATGAAGGCAGGTGAAGTTATACAAACTGATCTACTAAGCATTCTCATGGAATCCAATTTGAATGAAATTAAACAACATGGAAACAATAAAGACATTGGAATAAGCATAGAAGATGTTATTGCAGAGTGCAAGCTTTTCTATATTGCTGGCCAAGAAACTACAGCTACGTTACTAGTTTGGACGATGGTATTGTTGAGTTCATACTCAGAATGGCAAGAACAAGCAAGAGCAGAGGTCTTTGAGATCTTTGGTAACAAGAAACCAGATTATGATGGTTTAAGTCGACTAAAAGTTTTTTATCTCATATATGAAATTAAATGCCCATCTTTCGATATAAAGCAATCATATGAGAAAGTTTTGGAGGAATCAATACTGAATAGTCTAGAAAGGTTATACCCACCATTGAGTATGTTTGGTCATATGGTTAAGAAAGAAACAAGACTTGGAAATTTGACTTTACCAGCAGGAGTGATGTTGGGCATACCAAGTGTTCTTATCCAACATGATCCTAAGTCATGGGGAGAAGATGCAGATGAATTTAAACCAGAAAGATTTTTTGAAGGAGTTTCTAAAGCAACAAAAAATCTAAGTGCTTTCGTACCATTTGGTTGGGGTCCTCGAATATGCATAGGACTAAACTTTGCTATGATTGAAGCAAAAATGGCATTATCAATCATCCTACAAGGCTTCTCATTTGAGCTTTCGTCATCATATGCACACGCTCCCACAGCCGCCTTAACAACGCAACCTCAACATGGAGCTCATCTCATACTACACAAACTGTAG

Coding sequence (CDS)

ATGGATAGCAAGTATTCATTCATGTGGCTTGGACCCATACCAAGAGTGCATATCATGGACCCTGAACAACTTAAAATTGTGTTTTCATTGATCAATGATTTCCAAAAGCCAACTATAAACCCCCTTGTCAGGTTGCTTGCAAATGGACTTGCAAATCATGAAGGACCAAAATGGGTCAAACACAGAAAGATAATCAACCATGCATTTCATTTACAAAAGCTTAAGGATATGGTACCAGCATTTTATCACAGCTGTAACGAGATGATTAGTAAATGGGAAAGCATGGTCTTTGTAGAAGGATCTTGTGAGTTAGATGTAATGCCTTATCTACAAAATATGGCTGCTGATGTGATTTCTCGAACTGCATTTGGGAGATGGAGATTTCTACCCACAAAGTCAAACAATAAAATGAAAGAGATAAGTAAAGAGATAACAACTTTGATCTTGGGTATTATGAATGAAAGGGAAAAGTCAATGAAGGCAGGTGAAGTTATACAAACTGATCTACTAAGCATTCTCATGGAATCCAATTTGAATGAAATTAAACAACATGGAAACAATAAAGACATTGGAATAAGCATAGAAGATGTTATTGCAGAGTGCAAGCTTTTCTATATTGCTGGCCAAGAAACTACAGCTACGTTACTAGTTTGGACGATGGTATTGTTGAGTTCATACTCAGAATGGCAAGAACAAGCAAGAGCAGAGGTCTTTGAGATCTTTGGTAACAAGAAACCAGATTATGATGGTTTAAGTCGACTAAAAGTTTTTTATCTCATATATGAAATTAAATGCCCATCTTTCGATATAAAGCAATCATATGAGAAAGTTTTGGAGGAATCAATACTGAATAGTCTAGAAAGGTTATACCCACCATTGAGTATGTTTGGTCATATGGTTAAGAAAGAAACAAGACTTGGAAATTTGACTTTACCAGCAGGAGTGATGTTGGGCATACCAAGTGTTCTTATCCAACATGATCCTAAGTCATGGGGAGAAGATGCAGATGAATTTAAACCAGAAAGATTTTTTGAAGGAGTTTCTAAAGCAACAAAAAATCTAAGTGCTTTCGTACCATTTGGTTGGGGTCCTCGAATATGCATAGGACTAAACTTTGCTATGATTGAAGCAAAAATGGCATTATCAATCATCCTACAAGGCTTCTCATTTGAGCTTTCGTCATCATATGCACACGCTCCCACAGCCGCCTTAACAACGCAACCTCAACATGGAGCTCATCTCATACTACACAAACTGTAG

Protein sequence

MDSKYSFMWLGPIPRVHIMDPEQLKIVFSLINDFQKPTINPLVRLLANGLANHEGPKWVKHRKIINHAFHLQKLKDMVPAFYHSCNEMISKWESMVFVEGSCELDVMPYLQNMAADVISRTAFGRWRFLPTKSNNKMKEISKEITTLILGIMNEREKSMKAGEVIQTDLLSILMESNLNEIKQHGNNKDIGISIEDVIAECKLFYIAGQETTATLLVWTMVLLSSYSEWQEQARAEVFEIFGNKKPDYDGLSRLKVFYLIYEIKCPSFDIKQSYEKVLEESILNSLERLYPPLSMFGHMVKKETRLGNLTLPAGVMLGIPSVLIQHDPKSWGEDADEFKPERFFEGVSKATKNLSAFVPFGWGPRICIGLNFAMIEAKMALSIILQGFSFELSSSYAHAPTAALTTQPQHGAHLILHKL
BLAST of Lsi04G019600 vs. Swiss-Prot
Match: C7A29_PANGI (Cytochrome P450 CYP72A219 OS=Panax ginseng PE=2 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 1.8e-125
Identity = 230/447 (51.45%), Postives = 298/447 (66.67%), Query Frame = 1

Query: 4   KYSFMWLGPIPRVHIMDPEQLKIVFSLINDFQKPTINPLVRLLANGLANHEGPKWVKHRK 63
           K SF+WLGP P VHIM+P+ +K V S    FQKP  NPL +LLA G+A+ EG +W KHRK
Sbjct: 94  KNSFLWLGPKPMVHIMNPDHIKDVLSKFYQFQKPRHNPLTKLLATGVADAEGDRWAKHRK 153

Query: 64  IINHAFHLQKLKDMVPAFYHSCNEMISKWESMVFVEGSCELDVMPYLQNMAADVISRTAF 123
           +IN AFHL+KLK+M+PA Y S +E+++KWE MV  +G  ELDV+PYL+ + +DVISRTAF
Sbjct: 154 LINPAFHLEKLKNMLPAIYLSSSEIVTKWEEMVSTKGQFELDVLPYLETLTSDVISRTAF 213

Query: 124 GR------------------------------WRFLPTKSNNKMKEISKEITTLILGIMN 183
           G                                RFLPTK N +MKEI+KE+   +  I+N
Sbjct: 214 GSSYEEGRKIFQLQREQAELIIQASQTIYLPGMRFLPTKRNKRMKEIAKEVKIALKSIIN 273

Query: 184 EREKSMKAGE-VIQTDLLSILMESNLNEIKQHGNNKDIGISIEDVIAECKLFYIAGQETT 243
           +R K+M+AGE     DLL IL+ESN  EIKQHGN  + G+++++VI ECKLF+ AGQETT
Sbjct: 274 KRLKAMEAGERSSHDDLLGILLESNSKEIKQHGNT-NFGLTVDEVIEECKLFFFAGQETT 333

Query: 244 ATLLVWTMVLLSSYSEWQEQARAEVFEIFGNKKPDYDGLSRLKVFYLIYEIKCPSFDIKQ 303
           + LLVWTM+LLS + +WQ++A+ EV   FGN KPD+DGL+ LKV  +I            
Sbjct: 334 SNLLVWTMILLSQHQDWQKRAKEEVLRTFGNNKPDFDGLNHLKVVNMI------------ 393

Query: 304 SYEKVLEESILNSLERLYPPLSMFGHMVKKETRLGNLTLPAGVMLGIPSVLIQHDPKSWG 363
                     L  + RLYPP+      + +E +LG ++LPAGV+L +P +L+ +D + WG
Sbjct: 394 ----------LLEVLRLYPPILSLDRTIYEEIKLGEISLPAGVILLLPIILLHYDQEIWG 453

Query: 364 EDADEFKPERFFEGVSKATKNLSAFVPFGWGPRICIGLNFAMIEAKMALSIILQGFSFEL 420
           +DA EF PERF EGV KATK    + PF WGPRICIG NFAM+EAKMA+++ILQ FSF L
Sbjct: 454 DDAKEFNPERFSEGVLKATKGRVTYFPFSWGPRICIGQNFAMLEAKMAMAMILQRFSFVL 513

BLAST of Lsi04G019600 vs. Swiss-Prot
Match: C7263_MEDTR (11-oxo-beta-amyrin 30-oxidase OS=Medicago truncatula GN=CYP72A63 PE=1 SV=1)

HSP 1 Score: 444.5 bits (1142), Expect = 1.3e-123
Identity = 222/444 (50.00%), Postives = 293/444 (65.99%), Query Frame = 1

Query: 6   SFMWLGPIPRVHIMDPEQLKIVFSLINDFQKPTINPLVRLLANGLANHEGPKWVKHRKII 65
           SF+W G  PRV I DP+Q+K VF+ I+DF KP +  + + L+ G+ +HEG KW KHRKI 
Sbjct: 104 SFLWEGTTPRVIITDPDQIKDVFNKIDDFPKPKLRSIAKYLSVGILDHEGKKWAKHRKIA 163

Query: 66  NHAFHLQKLKDMVPAFYHSCNEMISKWESMVFVEGSCELDVMPYLQNMAADVISRTAFGR 125
           N AFHL+KLK M+PAF HSCNEMISKW+ ++  +G+CE+DV P LQN   DVISRTAFG 
Sbjct: 164 NPAFHLEKLKVMLPAFSHSCNEMISKWKELLSSDGTCEIDVWPSLQNFTCDVISRTAFGS 223

Query: 126 -----------------------------WRFLPTKSNNKMKEISKEITTLILGIMNERE 185
                                        W  L T +  KMKEI +EI   + GI+ +RE
Sbjct: 224 SYAEGTKLFQLLKKQGFLLMTGRHTNNPLWGLLATTTKTKMKEIDREIHDSLEGIIEKRE 283

Query: 186 KSMKAGEVIQTDLLSILMESNLNEIKQHGNNKDIGISIEDVIAECKLFYIAGQETTATLL 245
           K++K GE    DLL IL++SN  E +  GN+K+IG++ +DVI ECKLFY+AGQETT++LL
Sbjct: 284 KALKNGETTNDDLLGILLQSNHAEKQGQGNSKNIGMTTQDVIDECKLFYLAGQETTSSLL 343

Query: 246 VWTMVLLSSYSEWQEQARAEVFEIFGNKKPDYDGLSRLKVFYLI-YEIKCPSFDIKQSYE 305
           VWTMVLL  Y EWQ +AR EV ++FGN+ P+ +GLS+LK+  +I YE+            
Sbjct: 344 VWTMVLLGRYPEWQARAREEVLQVFGNQNPNNEGLSQLKIVTMILYEVL----------- 403

Query: 306 KVLEESILNSLERLYPPLSMFGHMVKKETRLGNLTLPAGVMLGIPSVLIQHDPKSWGEDA 365
                       RL+PPL  F   ++K+ +LGNL LP G  + +P +LI  D   WG+DA
Sbjct: 404 ------------RLFPPLIYFNRALRKDLKLGNLLLPEGTQISLPILLIHQDHDLWGDDA 463

Query: 366 DEFKPERFFEGVSKATKNLSAFVPFGWGPRICIGLNFAMIEAKMALSIILQGFSFELSSS 420
            EFKPERF EG++KATK   ++ PFGWGPRIC+G NFA++EAK+A+S++LQ FSFELS +
Sbjct: 464 KEFKPERFAEGIAKATKGQVSYFPFGWGPRICLGQNFALLEAKIAVSLLLQNFSFELSPN 523

BLAST of Lsi04G019600 vs. Swiss-Prot
Match: C7254_GLYUR (11-oxo-beta-amyrin 30-oxidase OS=Glycyrrhiza uralensis GN=CYP72A154 PE=1 SV=1)

HSP 1 Score: 442.2 bits (1136), Expect = 6.4e-123
Identity = 220/446 (49.33%), Postives = 290/446 (65.02%), Query Frame = 1

Query: 4   KYSFMWLGPIPRVHIMDPEQLKIVFSLINDFQKPTINPLVRLLANGLANHEGPKWVKHRK 63
           K SF W G  P+V I DPEQ+K VF+ I DF KP +NP+ + ++ GL  +EG KW KHRK
Sbjct: 101 KNSFAWEGTAPKVIITDPEQIKEVFNKIQDFPKPKLNPIAKYISIGLVQYEGDKWAKHRK 160

Query: 64  IINHAFHLQKLKDMVPAFYHSCNEMISKWESMVFVEGSCELDVMPYLQNMAADVISRTAF 123
           IIN AFHL+KLK M+PAF HSC+EMISKW+ ++  +G+CE+DV P+LQN+  DVISRTAF
Sbjct: 161 IINPAFHLEKLKGMLPAFSHSCHEMISKWKGLLSSDGTCEVDVWPFLQNLTCDVISRTAF 220

Query: 124 GR-----------------------------WRFLPTKSNNKMKEISKEITTLILGIMNE 183
           G                              W  LP+ +  +MKEI + I   + GI+ +
Sbjct: 221 GSSYAEGAKIFELLKRQGYALMTARYARIPLWWLLPSTTKRRMKEIERGIRDSLEGIIRK 280

Query: 184 REKSMKAGEVIQTDLLSILMESNLNEIKQHGNNKDIGISIEDVIAECKLFYIAGQETTAT 243
           REK++K+G+    DLL IL++SN  E K   N+K  G++ ++V+ ECKLFY+AGQETTA 
Sbjct: 281 REKALKSGKSTDDDLLGILLQSNHIENKGDENSKSAGMTTQEVMEECKLFYLAGQETTAA 340

Query: 244 LLVWTMVLLSSYSEWQEQARAEVFEIFGNKKPDYDGLSRLKVFYLI-YEIKCPSFDIKQS 303
           LL WTMVLL  + EWQ +AR EV ++FGN+ P+++GL RLK+  +I YE+          
Sbjct: 341 LLAWTMVLLGKHPEWQARARQEVLQVFGNQNPNFEGLGRLKIVTMILYEVL--------- 400

Query: 304 YEKVLEESILNSLERLYPPLSMFGHMVKKETRLGNLTLPAGVMLGIPSVLIQHDPKSWGE 363
                         RLYPP       ++K+ +LGNL LPAGV + +P +LI HD   WG 
Sbjct: 401 --------------RLYPPGIYLTRALRKDLKLGNLLLPAGVQVSVPILLIHHDEGIWGN 460

Query: 364 DADEFKPERFFEGVSKATKNLSAFVPFGWGPRICIGLNFAMIEAKMALSIILQGFSFELS 420
           DA EF PERF EG++KATK    + PFGWGPRIC+G NFA++EAK+ LS++LQ FSFELS
Sbjct: 461 DAKEFNPERFAEGIAKATKGQVCYFPFGWGPRICVGQNFALLEAKIVLSLLLQNFSFELS 520

BLAST of Lsi04G019600 vs. Swiss-Prot
Match: C7A15_ARATH (Cytochrome P450 72A15 OS=Arabidopsis thaliana GN=CYP72A15 PE=2 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 2.5e-119
Identity = 219/445 (49.21%), Postives = 296/445 (66.52%), Query Frame = 1

Query: 7   FMWLGPIPRVHIMDPEQLKIVFSLINDFQKPTINPLVRLLANGLANHEGPKWVKHRKIIN 66
           F W GPIP + IMDPEQ+K VF+ + DFQKP   PL  ++A GLAN++G KW KHR+IIN
Sbjct: 97  FTWFGPIPTITIMDPEQIKEVFNKVYDFQKPHTFPLATIIAKGLANYDGDKWAKHRRIIN 156

Query: 67  HAFHLQKLKDMVPAFYHSCNEMISKWESMVFVEGS-CELDVMPYLQNMAADVISRTAFGR 126
            AFH++K+K+MVPAF+ SC E++ +W+ +V  +GS CE+DV P L +M ADVISRTAFG 
Sbjct: 157 PAFHIEKIKNMVPAFHQSCREVVGEWDQLVSDKGSSCEVDVWPGLVSMTADVISRTAFGS 216

Query: 127 ------------------------------WRFLPTKSNNKMKEISKEITTLILGIMNER 186
                                         + +LPTKSN +MK  ++EI  ++ GI+N+R
Sbjct: 217 SYKEGQRIFELQAELAQLIIQAFRKAFIPGYSYLPTKSNRRMKAAAREIQVILRGIVNKR 276

Query: 187 EKSMKAGEVIQTDLLSILMESNLNEIKQHGNNKDIGISIEDVIAECKLFYIAGQETTATL 246
            ++ +AGE    DLL IL+ESNL + +  GN    G+S ED++ ECKLFY AGQETT+ L
Sbjct: 277 LRAREAGEAPSDDLLGILLESNLRQTE--GN----GMSTEDLMEECKLFYFAGQETTSVL 336

Query: 247 LVWTMVLLSSYSEWQEQARAEVFEIFGNKKPDYDGLSRLKVFYLI-YEIKCPSFDIKQSY 306
           LVWTMVLLS + +WQ +AR EV ++FG+K+PD +GL++LKV  +I YE+           
Sbjct: 337 LVWTMVLLSQHQDWQARAREEVKQVFGDKEPDAEGLNQLKVMTMILYEVL---------- 396

Query: 307 EKVLEESILNSLERLYPPLSMFGHMVKKETRLGNLTLPAGVMLGIPSVLIQHDPKSWGED 366
                        RLYPP++     + KE +LG+LTLP GV + +P +L+QHD + WG D
Sbjct: 397 -------------RLYPPVTQLTRAIHKELKLGDLTLPGGVQISLPILLVQHDIELWGND 456

Query: 367 ADEFKPERFFEGVSKATKNLSAFVPFGWGPRICIGLNFAMIEAKMALSIILQGFSFELSS 420
           A EF P+RF +G+SKATK+  +F PF WGPRICIG NFA++EAKMA+++IL+ FSFE+S 
Sbjct: 457 AAEFNPDRFKDGLSKATKSQVSFFPFAWGPRICIGQNFALLEAKMAMALILRRFSFEISP 512

BLAST of Lsi04G019600 vs. Swiss-Prot
Match: C7A13_ARATH (Cytochrome P450 72A13 OS=Arabidopsis thaliana GN=CYP72A13 PE=2 SV=1)

HSP 1 Score: 428.7 bits (1101), Expect = 7.3e-119
Identity = 220/444 (49.55%), Postives = 291/444 (65.54%), Query Frame = 1

Query: 7   FMWLGPIPRVHIMDPEQLKIVFSLINDFQKPTINPLVRLLANGLANHEGPKWVKHRKIIN 66
           F W GPIP + IMDPEQ+K VF+ + DFQK    PL RL+A GL +++G KW KHR+IIN
Sbjct: 97  FTWFGPIPTITIMDPEQIKEVFNKVYDFQKAHTFPLGRLIAAGLVSYDGDKWTKHRRIIN 156

Query: 67  HAFHLQKLKDMVPAFYHSCNEMISKWESMVF-VEGSCELDVMPYLQNMAADVISRTAFGR 126
            AFHL+K+K+MVPAF+ SC+E++ +W+ +V   + SCE+D+ P+L +M ADVISRTAFG 
Sbjct: 157 PAFHLEKIKNMVPAFHQSCSEIVGEWDKLVTDKQSSCEVDIWPWLVSMTADVISRTAFGS 216

Query: 127 ------------------------------WRFLPTKSNNKMKEISKEITTLILGIMNER 186
                                         +R+ PTK N +MK  ++EI  ++ GI+N+R
Sbjct: 217 SYKEGQRIFELQAELAQLIIQAFRKAIIPGYRYFPTKGNRRMKAAAREIKFILRGIVNKR 276

Query: 187 EKSMKAGEVIQTDLLSILMESNLNEIKQHGNNKDIGISIEDVIAECKLFYIAGQETTATL 246
            ++ +AGE    DLL IL+ESNL      G  K  G+S E+++ ECKLFY AGQETT  L
Sbjct: 277 LRAREAGEAPSDDLLGILLESNL------GQTKGNGMSTEELMEECKLFYFAGQETTTVL 336

Query: 247 LVWTMVLLSSYSEWQEQARAEVFEIFGNKKPDYDGLSRLKVFYLIYEIKCPSFDIKQSYE 306
           LVWTMVLLS + +WQ +AR EV ++FG+K+PD +GL++LKV  +                
Sbjct: 337 LVWTMVLLSQHQDWQARAREEVKQVFGDKEPDAEGLNQLKVMTM---------------- 396

Query: 307 KVLEESILNSLERLYPPLSMFGHMVKKETRLGNLTLPAGVMLGIPSVLIQHDPKSWGEDA 366
                 IL  + RLYPP+      + KE +LG+LTLP GV + +P +LIQ D + WG DA
Sbjct: 397 ------ILYEVLRLYPPVVQLTRAIHKEMQLGDLTLPGGVQISLPILLIQRDRELWGNDA 456

Query: 367 DEFKPERFFEGVSKATKNLSAFVPFGWGPRICIGLNFAMIEAKMALSIILQGFSFELSSS 420
            EFKP+RF +G+SKATKN  +F PF WGPRICIG NFA++EAKMA+++IL+ FSFELS S
Sbjct: 457 GEFKPDRFKDGLSKATKNQVSFFPFAWGPRICIGQNFALLEAKMAMTLILRKFSFELSPS 512

BLAST of Lsi04G019600 vs. TrEMBL
Match: W9QZH6_9ROSA (Secologanin synthase OS=Morus notabilis GN=L484_024637 PE=3 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 8.9e-140
Identity = 253/445 (56.85%), Postives = 315/445 (70.79%), Query Frame = 1

Query: 4   KYSFMWLGPIPRVHIMDPEQLKIVFSLINDFQKPTINPLVRLLANGLANHEGPKWVKHRK 63
           K SFMW+GP PRV I +PE +K +F+ + DF KP   PLV+LLA G+A +EG KW +HRK
Sbjct: 95  KDSFMWIGPTPRVSITNPEYIKDIFNKLGDFPKPQSIPLVKLLATGVATYEGEKWTRHRK 154

Query: 64  IINHAFHLQKLKDMVPAFYHSCNEMISKWESMVFVEGSCELDVMPYLQNMAADVISRTAF 123
           IIN AFH++KLK +VPAFY SC+EM++KWE ++  EGSCELDV  YLQNM  DVISRTAF
Sbjct: 155 IINPAFHMEKLKRLVPAFYLSCSEMLNKWERLLNSEGSCELDVWSYLQNMTGDVISRTAF 214

Query: 124 GR------------------------------WRFLPTKSNNKMKEISKEITTLILGIMN 183
           G                               WRF+PTK N +MK+I  EI  L+  I++
Sbjct: 215 GSSYEEGRRIFQLQKEQGELAIKSLQTVYIPGWRFVPTKLNRRMKKIYNEIEALVKNIIH 274

Query: 184 EREKSMKAGEVIQTDLLSILMESNLNEIKQHGNNKDIGISIEDVIAECKLFYIAGQETTA 243
           +RE +M+AGE    DLL ILMESNL EI++HGNNK++GISI DVI ECKLFYIAGQETT+
Sbjct: 275 KREGAMRAGEAPNDDLLGILMESNLKEIQEHGNNKNMGISIRDVIEECKLFYIAGQETTS 334

Query: 244 TLLVWTMVLLSSYSEWQEQARAEVFEIFGNKKPDYDGLSRLKVFYLIYEIKCPSFDIKQS 303
           TLLVWTMVLLS +  WQ++AR EV +IFGN KP +DGL++LKV  +I+            
Sbjct: 335 TLLVWTMVLLSRFPSWQDRAREEVLQIFGNNKPGFDGLTQLKVVTMIF------------ 394

Query: 304 YEKVLEESILNSLERLYPPLSMFGHMVKKETRLGNLTLPAGVMLGIPSVLIQHDPKSWGE 363
           YE +          RLYPPL +F   V+K+T+LGNL+LPAGV + IP++L+  D + WGE
Sbjct: 395 YEVL----------RLYPPLILFTRKVEKKTQLGNLSLPAGVEVSIPTILVHRDTELWGE 454

Query: 364 DADEFKPERFFEGVSKATKNLSAFVPFGWGPRICIGLNFAMIEAKMALSIILQGFSFELS 419
           DA +FKPERF EG+SK TK   +F PFGWGPRIC+G NFAM+EAKMAL++ILQ F+FELS
Sbjct: 455 DAKDFKPERFAEGMSKVTKGQVSFFPFGWGPRICVGQNFAMVEAKMALALILQRFTFELS 514

BLAST of Lsi04G019600 vs. TrEMBL
Match: I1MIY4_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_15G244200 PE=3 SV=1)

HSP 1 Score: 497.3 bits (1279), Expect = 1.9e-137
Identity = 246/448 (54.91%), Postives = 319/448 (71.21%), Query Frame = 1

Query: 4   KYSFMWLGPIPRVHIMDPEQLKIVFSLINDFQKPTINPLVRLLANGLANHEGPKWVKHRK 63
           K SF+WLGPIPRV I+DPEQ+K VF+ I DF KP +NPLV+LLA GLA +EG KW KHR+
Sbjct: 93  KNSFIWLGPIPRVTILDPEQIKDVFNKIYDFPKPNMNPLVKLLATGLAGYEGEKWSKHRR 152

Query: 64  IINHAFHLQKLKDMVPAFYHSCNEMISKWESMVFVEGSCELDVMPYLQNMAADVISRTAF 123
           IIN AF+L+KLK M+P F+ SCN+++SKWE M+  EGSCE+D  P+LQN+A+DVI+R+AF
Sbjct: 153 IINPAFNLEKLKIMLPLFFKSCNDLVSKWEGMLSSEGSCEMDAWPFLQNLASDVIARSAF 212

Query: 124 GR------------------------------WRFLPTKSNNKMKEISKEITTLILGIMN 183
           G                               WRFLPT ++ +MKEI ++I   +  ++N
Sbjct: 213 GSSYEEGRRIFQLQREQAELLIKVLLKIQIPGWRFLPTNTHRRMKEIDRDIKASLKDMIN 272

Query: 184 EREKSMKAGEVIQTDLLSILMESNLNEIKQHGN--NKDIGISIEDVIAECKLFYIAGQET 243
           +REK++KAGE  + DLL IL+ESN  EI++HGN  NK++G+S+E+VI ECKLFY AGQET
Sbjct: 273 KREKALKAGEATKNDLLGILLESNHKEIQEHGNRNNKNVGMSLEEVIEECKLFYFAGQET 332

Query: 244 TATLLVWTMVLLSSYSEWQEQARAEVFEIFGNKKPDYDGLSRLKVFYLIYEIKCPSFDIK 303
           T+ LLVWTMVLLS Y +WQ +AR EVF++FG +KPD+DGLSRLK+  +            
Sbjct: 333 TSVLLVWTMVLLSRYPDWQARAREEVFQVFGYQKPDFDGLSRLKIVTM------------ 392

Query: 304 QSYEKVLEESILNSLERLYPPLSMFGHMVKKETRLGNLTLPAGVMLGIPSVLIQHDPKSW 363
                     IL  + RLYPP++     ++K+ +LG LTLPAGV + +P++LI HD K W
Sbjct: 393 ----------ILYEVLRLYPPVAGMTRSIEKDVKLGTLTLPAGVHVLLPTILIHHDRKFW 452

Query: 364 GEDADEFKPERFFEGVSKATKNLSAFVPFGWGPRICIGLNFAMIEAKMALSIILQGFSFE 420
           GEDA +F PERF EGV KAT    +F PFGWGPRICIG NF+++EAKMALS+ILQ FSFE
Sbjct: 453 GEDAKQFNPERFSEGVLKATNGRVSFFPFGWGPRICIGQNFSLLEAKMALSMILQHFSFE 512

BLAST of Lsi04G019600 vs. TrEMBL
Match: A0A0B2SBC8_GLYSO (Secologanin synthase OS=Glycine soja GN=glysoja_039974 PE=3 SV=1)

HSP 1 Score: 494.6 bits (1272), Expect = 1.2e-136
Identity = 244/448 (54.46%), Postives = 316/448 (70.54%), Query Frame = 1

Query: 4   KYSFMWLGPIPRVHIMDPEQLKIVFSLINDFQKPTINPLVRLLANGLANHEGPKWVKHRK 63
           K SF+W GP+PRV I+DPEQ+K VF+   DF KP +NPLV+LLA GLA +EG KW KHR+
Sbjct: 93  KNSFIWFGPMPRVTILDPEQIKDVFNKNYDFPKPNLNPLVKLLATGLAGYEGEKWSKHRR 152

Query: 64  IINHAFHLQKLKDMVPAFYHSCNEMISKWESMVFVEGSCELDVMPYLQNMAADVISRTAF 123
           IIN AF+L+KLK M+P F  SCN+++SKWE M+  EGSCE+D  P+LQN+ +DVI+R+AF
Sbjct: 153 IINPAFNLEKLKIMLPLFLQSCNDLVSKWEGMLSSEGSCEMDAWPFLQNLTSDVIARSAF 212

Query: 124 GR------------------------------WRFLPTKSNNKMKEISKEITTLILGIMN 183
           G                               WRFLPTK++ +MKEI +EI   +  ++N
Sbjct: 213 GSSYEEGRRIFQLQREQTELLMKVILKIQIPGWRFLPTKTHRRMKEIDREIKASLKNMIN 272

Query: 184 EREKSMKAGEVIQTDLLSILMESNLNEIKQHGN--NKDIGISIEDVIAECKLFYIAGQET 243
           +REK++K+GE  + DLL IL+ESN  EI++HGN  +K++G+S+EDVI ECKLFY AGQET
Sbjct: 273 KREKALKSGEATKNDLLGILLESNHKEIQEHGNRNSKNVGMSLEDVIEECKLFYFAGQET 332

Query: 244 TATLLVWTMVLLSSYSEWQEQARAEVFEIFGNKKPDYDGLSRLKVFYLIYEIKCPSFDIK 303
           T+ LLVWTMVLLS Y +WQ +AR E F++FG +KPD+DGLSRLK+  +            
Sbjct: 333 TSVLLVWTMVLLSRYPDWQARAREEAFQVFGYQKPDFDGLSRLKIVTM------------ 392

Query: 304 QSYEKVLEESILNSLERLYPPLSMFGHMVKKETRLGNLTLPAGVMLGIPSVLIQHDPKSW 363
                     IL  + RLYPPL     +V+K+ +LGNLTLPAGV + +P+VLI HD + W
Sbjct: 393 ----------ILYEVLRLYPPLIGMNRLVEKDVKLGNLTLPAGVQVFLPTVLIHHDSELW 452

Query: 364 GEDADEFKPERFFEGVSKATKNLSAFVPFGWGPRICIGLNFAMIEAKMALSIILQGFSFE 420
           GEDA +F PERF EGV KAT    +F PFGWGPRICIG NF+++EAKMALS+ILQ F FE
Sbjct: 453 GEDAKQFNPERFSEGVLKATNGRVSFFPFGWGPRICIGQNFSLLEAKMALSMILQNFLFE 512

BLAST of Lsi04G019600 vs. TrEMBL
Match: I1MIY5_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_15G244300 PE=3 SV=1)

HSP 1 Score: 494.6 bits (1272), Expect = 1.2e-136
Identity = 244/448 (54.46%), Postives = 316/448 (70.54%), Query Frame = 1

Query: 4   KYSFMWLGPIPRVHIMDPEQLKIVFSLINDFQKPTINPLVRLLANGLANHEGPKWVKHRK 63
           K SF+W GP+PRV I+DPEQ+K VF+   DF KP +NPLV+LLA GLA +EG KW KHR+
Sbjct: 93  KNSFIWFGPMPRVTILDPEQIKDVFNKNYDFPKPNLNPLVKLLATGLAGYEGEKWSKHRR 152

Query: 64  IINHAFHLQKLKDMVPAFYHSCNEMISKWESMVFVEGSCELDVMPYLQNMAADVISRTAF 123
           IIN AF+L+KLK M+P F  SCN+++SKWE M+  EGSCE+D  P+LQN+ +DVI+R+AF
Sbjct: 153 IINPAFNLEKLKIMLPLFLQSCNDLVSKWEGMLSSEGSCEMDAWPFLQNLTSDVIARSAF 212

Query: 124 GR------------------------------WRFLPTKSNNKMKEISKEITTLILGIMN 183
           G                               WRFLPTK++ +MKEI +EI   +  ++N
Sbjct: 213 GSSYEEGRRIFQLQREQTEHLMKVILKIQIPGWRFLPTKTHRRMKEIDREIKASLKNMIN 272

Query: 184 EREKSMKAGEVIQTDLLSILMESNLNEIKQHGN--NKDIGISIEDVIAECKLFYIAGQET 243
           +REK++K+GE  + DLL IL+ESN  EI++HGN  +K++G+S+EDVI ECKLFY AGQET
Sbjct: 273 KREKALKSGEATKNDLLGILLESNHKEIQEHGNRNSKNVGMSLEDVIEECKLFYFAGQET 332

Query: 244 TATLLVWTMVLLSSYSEWQEQARAEVFEIFGNKKPDYDGLSRLKVFYLIYEIKCPSFDIK 303
           T+ LLVWTMVLLS Y +WQ +AR E F++FG +KPD+DGLSRLK+  +            
Sbjct: 333 TSVLLVWTMVLLSRYPDWQARAREEAFQVFGYQKPDFDGLSRLKIVTM------------ 392

Query: 304 QSYEKVLEESILNSLERLYPPLSMFGHMVKKETRLGNLTLPAGVMLGIPSVLIQHDPKSW 363
                     IL  + RLYPPL     +V+K+ +LGNLTLPAGV + +P+VLI HD + W
Sbjct: 393 ----------ILYEVLRLYPPLIGMNRLVEKDVKLGNLTLPAGVQVFLPTVLIHHDSELW 452

Query: 364 GEDADEFKPERFFEGVSKATKNLSAFVPFGWGPRICIGLNFAMIEAKMALSIILQGFSFE 420
           GEDA +F PERF EGV KAT    +F PFGWGPRICIG NF+++EAKMALS+ILQ F FE
Sbjct: 453 GEDAKQFNPERFSEGVLKATNGRVSFFPFGWGPRICIGQNFSLLEAKMALSMILQNFLFE 512

BLAST of Lsi04G019600 vs. TrEMBL
Match: M5WPE9_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004271mg PE=3 SV=1)

HSP 1 Score: 494.2 bits (1271), Expect = 1.6e-136
Identity = 246/445 (55.28%), Postives = 312/445 (70.11%), Query Frame = 1

Query: 4   KYSFMWLGPIPRVHIMDPEQLKIVFSLINDFQKPTINPLVRLLANGLANHEGPKWVKHRK 63
           K SF+W+GP PRV+I +PE +K +F+   DFQKP  NPLV+LLA GLAN+EG KW KHRK
Sbjct: 96  KNSFIWIGPTPRVNISNPEDMKDIFAKYEDFQKPVKNPLVKLLATGLANYEGEKWAKHRK 155

Query: 64  IINHAFHLQKLKDMVPAFYHSCNEMISKWESMVFVEGSCELDVMPYLQNMAADVISRTAF 123
           IIN AFH +KLK M+PAF+ SC+E+I +WES+   EGSCELDV PYLQN+ ADVISRTAF
Sbjct: 156 IINPAFHSEKLKRMLPAFHQSCSELIEEWESLASKEGSCELDVWPYLQNLTADVISRTAF 215

Query: 124 GR------------------------------WRFLPTKSNNKMKEISKEITTLILGIMN 183
           G                               WRFLPT+ N +MKEI KEI   ++GI+N
Sbjct: 216 GSSYEEGRKIFQLLKDQAQLTIKAIQSVYIPGWRFLPTRMNKRMKEIDKEIKGSLMGIIN 275

Query: 184 EREKSMKAGEVIQTDLLSILMESNLNEIKQHGNNKDIGISIEDVIAECKLFYIAGQETTA 243
           +RE++++  E  + DLL ILM+SNL EI+++GN K++G+SIEDVI ECKLFY AGQETT+
Sbjct: 276 KREEAIRVREATRDDLLGILMDSNLKEIRENGNTKNVGMSIEDVIEECKLFYFAGQETTS 335

Query: 244 TLLVWTMVLLSSYSEWQEQARAEVFEIFGNKKPDYDGLSRLKVFYLIYEIKCPSFDIKQS 303
            LLVWT+VLLS   +WQ +AR EV ++FG+ KPD+DGLS LKV  +I             
Sbjct: 336 VLLVWTLVLLSQNQDWQARARDEVLQVFGSNKPDFDGLSHLKVVTMI------------- 395

Query: 304 YEKVLEESILNSLERLYPPLSMFGHMVKKETRLGNLTLPAGVMLGIPSVLIQHDPKSWGE 363
                    L  + RLYP + +    + K T+LG L+LPAGV + + ++L+ HD + WGE
Sbjct: 396 ---------LLEVLRLYPAVVVLSRAIHKNTQLGKLSLPAGVEVSLNTLLVHHDKELWGE 455

Query: 364 DADEFKPERFFEGVSKATKNLSAFVPFGWGPRICIGLNFAMIEAKMALSIILQGFSFELS 419
           DA+EFKPERF  GVSKATKN  A+ PFG GPRICIG NFAM+EAK+AL++ILQ F+FELS
Sbjct: 456 DANEFKPERFSSGVSKATKNKFAYFPFGGGPRICIGQNFAMVEAKLALALILQHFAFELS 515

BLAST of Lsi04G019600 vs. TAIR10
Match: AT3G14610.1 (AT3G14610.1 cytochrome P450, family 72, subfamily A, polypeptide 7)

HSP 1 Score: 445.3 bits (1144), Expect = 4.2e-125
Identity = 226/448 (50.45%), Postives = 299/448 (66.74%), Query Frame = 1

Query: 4   KYSFMWLGPIPRVHIMDPEQLKIVFSLINDFQKPTINPLVRLLANGLANHEGPKWVKHRK 63
           K  F+W+GP+P + I +PEQ+K VF+ +NDF+K +  PL+RLLA GLA+++G KW  HR+
Sbjct: 93  KTFFIWIGPLPTIVITNPEQIKEVFNKVNDFEKASTFPLIRLLAGGLASYKGDKWASHRR 152

Query: 64  IINHAFHLQKLKDMVPAFYHSCNEMISKWESMVF-VEGSCELDVMPYLQNMAADVISRTA 123
           IIN AFHL+K+K+M+PAFYH C+E++ +WE +    E   E+DV P+L NM ADVIS TA
Sbjct: 153 IINPAFHLEKIKNMIPAFYHCCSEVVCQWEKLFTDKESPLEVDVWPWLVNMTADVISHTA 212

Query: 124 FGRW------------------------------RFLPTKSNNKMKEISKEITTLILGIM 183
           FG                                RF PTKSN +MK I +E+  ++ GI+
Sbjct: 213 FGSSYKEGQRIFQLQGELAELIAQAFKKSYIPGSRFYPTKSNRRMKAIDREVDVILRGIV 272

Query: 184 NEREKSMKAGEVIQTDLLSILMESNLNEIKQHGNNKDIGISIEDVIAECKLFYIAGQETT 243
           ++REK+ +AGE    DLL IL+ESN  E    GN    G+S+EDV+ ECKLFY AGQETT
Sbjct: 273 SKREKAREAGEPANDDLLGILLESNSEE--SQGN----GMSVEDVMKECKLFYFAGQETT 332

Query: 244 ATLLVWTMVLLSSYSEWQEQARAEVFEIFG-NKKPDYDGLSRLKVFYLIYEIKCPSFDIK 303
           + LLVWTMVLLS + +WQ +AR EV ++ G N KPD + L+ LKV  +I+          
Sbjct: 333 SVLLVWTMVLLSHHQDWQARAREEVMQVLGENNKPDMESLNNLKVMTMIF---------- 392

Query: 304 QSYEKVLEESILNSLERLYPPLSMFGHMVKKETRLGNLTLPAGVMLGIPSVLIQHDPKSW 363
                       N + RLYPP++    +V KE +LG LTLPAG+ + +P++L+Q D + W
Sbjct: 393 ------------NEVLRLYPPVAQLKRVVNKEMKLGELTLPAGIQIYLPTILVQRDTELW 452

Query: 364 GEDADEFKPERFFEGVSKATKNLSAFVPFGWGPRICIGLNFAMIEAKMALSIILQGFSFE 420
           G+DA +FKPERF +G+SKATKN  +F PFGWGPRICIG NFAM+EAKMA+++ILQ FSFE
Sbjct: 453 GDDAADFKPERFRDGLSKATKNQVSFFPFGWGPRICIGQNFAMLEAKMAMALILQKFSFE 512

BLAST of Lsi04G019600 vs. TAIR10
Match: AT3G14690.1 (AT3G14690.1 cytochrome P450, family 72, subfamily A, polypeptide 15)

HSP 1 Score: 430.3 bits (1105), Expect = 1.4e-120
Identity = 219/445 (49.21%), Postives = 296/445 (66.52%), Query Frame = 1

Query: 7   FMWLGPIPRVHIMDPEQLKIVFSLINDFQKPTINPLVRLLANGLANHEGPKWVKHRKIIN 66
           F W GPIP + IMDPEQ+K VF+ + DFQKP   PL  ++A GLAN++G KW KHR+IIN
Sbjct: 97  FTWFGPIPTITIMDPEQIKEVFNKVYDFQKPHTFPLATIIAKGLANYDGDKWAKHRRIIN 156

Query: 67  HAFHLQKLKDMVPAFYHSCNEMISKWESMVFVEGS-CELDVMPYLQNMAADVISRTAFGR 126
            AFH++K+K+MVPAF+ SC E++ +W+ +V  +GS CE+DV P L +M ADVISRTAFG 
Sbjct: 157 PAFHIEKIKNMVPAFHQSCREVVGEWDQLVSDKGSSCEVDVWPGLVSMTADVISRTAFGS 216

Query: 127 ------------------------------WRFLPTKSNNKMKEISKEITTLILGIMNER 186
                                         + +LPTKSN +MK  ++EI  ++ GI+N+R
Sbjct: 217 SYKEGQRIFELQAELAQLIIQAFRKAFIPGYSYLPTKSNRRMKAAAREIQVILRGIVNKR 276

Query: 187 EKSMKAGEVIQTDLLSILMESNLNEIKQHGNNKDIGISIEDVIAECKLFYIAGQETTATL 246
            ++ +AGE    DLL IL+ESNL + +  GN    G+S ED++ ECKLFY AGQETT+ L
Sbjct: 277 LRAREAGEAPSDDLLGILLESNLRQTE--GN----GMSTEDLMEECKLFYFAGQETTSVL 336

Query: 247 LVWTMVLLSSYSEWQEQARAEVFEIFGNKKPDYDGLSRLKVFYLI-YEIKCPSFDIKQSY 306
           LVWTMVLLS + +WQ +AR EV ++FG+K+PD +GL++LKV  +I YE+           
Sbjct: 337 LVWTMVLLSQHQDWQARAREEVKQVFGDKEPDAEGLNQLKVMTMILYEVL---------- 396

Query: 307 EKVLEESILNSLERLYPPLSMFGHMVKKETRLGNLTLPAGVMLGIPSVLIQHDPKSWGED 366
                        RLYPP++     + KE +LG+LTLP GV + +P +L+QHD + WG D
Sbjct: 397 -------------RLYPPVTQLTRAIHKELKLGDLTLPGGVQISLPILLVQHDIELWGND 456

Query: 367 ADEFKPERFFEGVSKATKNLSAFVPFGWGPRICIGLNFAMIEAKMALSIILQGFSFELSS 420
           A EF P+RF +G+SKATK+  +F PF WGPRICIG NFA++EAKMA+++IL+ FSFE+S 
Sbjct: 457 AAEFNPDRFKDGLSKATKSQVSFFPFAWGPRICIGQNFALLEAKMAMALILRRFSFEISP 512

BLAST of Lsi04G019600 vs. TAIR10
Match: AT3G14660.1 (AT3G14660.1 cytochrome P450, family 72, subfamily A, polypeptide 13)

HSP 1 Score: 428.7 bits (1101), Expect = 4.1e-120
Identity = 220/444 (49.55%), Postives = 291/444 (65.54%), Query Frame = 1

Query: 7   FMWLGPIPRVHIMDPEQLKIVFSLINDFQKPTINPLVRLLANGLANHEGPKWVKHRKIIN 66
           F W GPIP + IMDPEQ+K VF+ + DFQK    PL RL+A GL +++G KW KHR+IIN
Sbjct: 97  FTWFGPIPTITIMDPEQIKEVFNKVYDFQKAHTFPLGRLIAAGLVSYDGDKWTKHRRIIN 156

Query: 67  HAFHLQKLKDMVPAFYHSCNEMISKWESMVF-VEGSCELDVMPYLQNMAADVISRTAFGR 126
            AFHL+K+K+MVPAF+ SC+E++ +W+ +V   + SCE+D+ P+L +M ADVISRTAFG 
Sbjct: 157 PAFHLEKIKNMVPAFHQSCSEIVGEWDKLVTDKQSSCEVDIWPWLVSMTADVISRTAFGS 216

Query: 127 ------------------------------WRFLPTKSNNKMKEISKEITTLILGIMNER 186
                                         +R+ PTK N +MK  ++EI  ++ GI+N+R
Sbjct: 217 SYKEGQRIFELQAELAQLIIQAFRKAIIPGYRYFPTKGNRRMKAAAREIKFILRGIVNKR 276

Query: 187 EKSMKAGEVIQTDLLSILMESNLNEIKQHGNNKDIGISIEDVIAECKLFYIAGQETTATL 246
            ++ +AGE    DLL IL+ESNL      G  K  G+S E+++ ECKLFY AGQETT  L
Sbjct: 277 LRAREAGEAPSDDLLGILLESNL------GQTKGNGMSTEELMEECKLFYFAGQETTTVL 336

Query: 247 LVWTMVLLSSYSEWQEQARAEVFEIFGNKKPDYDGLSRLKVFYLIYEIKCPSFDIKQSYE 306
           LVWTMVLLS + +WQ +AR EV ++FG+K+PD +GL++LKV  +                
Sbjct: 337 LVWTMVLLSQHQDWQARAREEVKQVFGDKEPDAEGLNQLKVMTM---------------- 396

Query: 307 KVLEESILNSLERLYPPLSMFGHMVKKETRLGNLTLPAGVMLGIPSVLIQHDPKSWGEDA 366
                 IL  + RLYPP+      + KE +LG+LTLP GV + +P +LIQ D + WG DA
Sbjct: 397 ------ILYEVLRLYPPVVQLTRAIHKEMQLGDLTLPGGVQISLPILLIQRDRELWGNDA 456

Query: 367 DEFKPERFFEGVSKATKNLSAFVPFGWGPRICIGLNFAMIEAKMALSIILQGFSFELSSS 420
            EFKP+RF +G+SKATKN  +F PF WGPRICIG NFA++EAKMA+++IL+ FSFELS S
Sbjct: 457 GEFKPDRFKDGLSKATKNQVSFFPFAWGPRICIGQNFALLEAKMAMTLILRKFSFELSPS 512

BLAST of Lsi04G019600 vs. TAIR10
Match: AT3G14630.1 (AT3G14630.1 cytochrome P450, family 72, subfamily A, polypeptide 9)

HSP 1 Score: 421.8 bits (1083), Expect = 5.0e-118
Identity = 220/448 (49.11%), Postives = 286/448 (63.84%), Query Frame = 1

Query: 4   KYSFMWLGPIPRVHIMDPEQLKIVFSLINDFQKPTINPLVRLLANGLANHEGPKWVKHRK 63
           K  F W GPIP + IM+P+ +K V++   DF+K    PL  LL +GLAN +G KWVKHRK
Sbjct: 90  KTFFTWSGPIPAITIMNPQLIKEVYNKFYDFEKTHTFPLTSLLTDGLANADGDKWVKHRK 149

Query: 64  IINHAFHLQKLKDMVPAFYHSCNEMISKWESMVFVEGS-CELDVMPYLQNMAADVISRTA 123
           IIN AFH +K+K+MVP FY SC E++ +WE +V  +GS CELDV P++ NM  DVISRTA
Sbjct: 150 IINPAFHFEKIKNMVPTFYKSCIEVMCEWEKLVSDKGSSCELDVWPWIVNMTGDVISRTA 209

Query: 124 FG------------------------------RWRFLPTKSNNKMKEISKEITTLILGIM 183
           FG                               +R  PTK+N +MK I KEI  ++ GI+
Sbjct: 210 FGSSYKEGQRIFILQAELAHLIILALGKNYIPAYRHFPTKNNRRMKTIVKEIQVILRGII 269

Query: 184 NEREKSMKAGEVIQTDLLSILMESNLNEIKQHGNNKDIGISIEDVIAECKLFYIAGQETT 243
           + REK+  AGE    DLL IL++SN  + K  GN    G+++E+++ ECKLFY AGQETT
Sbjct: 270 SHREKARDAGEAPSDDLLGILLKSNSEQSK--GN----GLNMEEIMEECKLFYFAGQETT 329

Query: 244 ATLLVWTMVLLSSYSEWQEQARAEVFEIFGNKKPDYDGLSRLKVF-YLIYEIKCPSFDIK 303
           + LL WTMVLLS + +WQ +AR EV ++FG+ KPD  G+++LKV   +IYE+        
Sbjct: 330 SVLLAWTMVLLSQHQDWQARAREEVMQVFGHNKPDLQGINQLKVMTMIIYEVL------- 389

Query: 304 QSYEKVLEESILNSLERLYPPLSMFGHMVKKETRLGNLTLPAGVMLGIPSVLIQHDPKSW 363
                           RLYPP+        KE +LG++TLP G+ + +P +LI  D K W
Sbjct: 390 ----------------RLYPPVIQMNRATHKEIKLGDMTLPGGIQVHMPVLLIHRDTKLW 449

Query: 364 GEDADEFKPERFFEGVSKATKNLSAFVPFGWGPRICIGLNFAMIEAKMALSIILQGFSFE 420
           G+DA EFKPERF +G++KATKN   F+PFGWGPRICIG NFA++EAKMAL++ILQ FSFE
Sbjct: 450 GDDAAEFKPERFKDGIAKATKNQVCFLPFGWGPRICIGQNFALLEAKMALALILQRFSFE 508

BLAST of Lsi04G019600 vs. TAIR10
Match: AT3G14680.1 (AT3G14680.1 cytochrome P450, family 72, subfamily A, polypeptide 14)

HSP 1 Score: 421.4 bits (1082), Expect = 6.5e-118
Identity = 214/442 (48.42%), Postives = 288/442 (65.16%), Query Frame = 1

Query: 9   WLGPIPRVHIMDPEQLKIVFSLINDFQKPTINPLVRLLANGLANHEGPKWVKHRKIINHA 68
           W GPIP + IMDPEQ+K VF+ + DFQK    PL ++L  GL +++G KW +HR+IIN A
Sbjct: 99  WFGPIPTITIMDPEQIKEVFNKVYDFQKAHTFPLSKILGTGLVSYDGDKWAQHRRIINPA 158

Query: 69  FHLQKLKDMVPAFYHSCNEMISKWESMVFVEGS-CELDVMPYLQNMAADVISRTAFGR-- 128
           FHL+K+K+MV  F+ SC+E++ +W+ +V  +GS CE+DV P L +M ADVISRTAFG   
Sbjct: 159 FHLEKIKNMVHVFHESCSELVGEWDKLVSDKGSSCEVDVWPGLTSMTADVISRTAFGSSY 218

Query: 129 ----------------------------WRFLPTKSNNKMKEISKEITTLILGIMNEREK 188
                                       + +LPTK N +MK  ++EI  ++ GI+N+RE+
Sbjct: 219 REGHRIFELQAELAQLVMQAFQKFFIPGYIYLPTKGNRRMKTAAREIQDILRGIINKRER 278

Query: 189 SMKAGEVIQTDLLSILMESNLNEIKQHGNNKDIGISIEDVIAECKLFYIAGQETTATLLV 248
           + ++GE    DLL IL+ESNL      G  +  G+S ED++ ECKLFY+AGQETT+ LLV
Sbjct: 279 ARESGEAPSEDLLGILLESNL------GQTEGNGMSTEDMMEECKLFYLAGQETTSVLLV 338

Query: 249 WTMVLLSSYSEWQEQARAEVFEIFGNKKPDYDGLSRLKVFYLIYEIKCPSFDIKQSYEKV 308
           WTMVLLS + +WQ +AR EV ++FG+K+PD +GL++LKV  +                  
Sbjct: 339 WTMVLLSQHQDWQARAREEVKQVFGDKQPDTEGLNQLKVMTM------------------ 398

Query: 309 LEESILNSLERLYPPLSMFGHMVKKETRLGNLTLPAGVMLGIPSVLIQHDPKSWGEDADE 368
               IL  + RLYPP+      + KE +LG+LTLP GV + +P +L+  D + WG DA E
Sbjct: 399 ----ILYEVLRLYPPVVQLTRAIHKEMKLGDLTLPGGVQISLPVLLVHRDTELWGNDAGE 458

Query: 369 FKPERFFEGVSKATKNLSAFVPFGWGPRICIGLNFAMIEAKMALSIILQGFSFELSSSYA 420
           FKPERF +G+SKATKN  +F PF WGPRICIG NF ++EAKMA+S+ILQ FSFELS SY 
Sbjct: 459 FKPERFKDGLSKATKNQVSFFPFAWGPRICIGQNFTLLEAKMAMSLILQRFSFELSPSYV 512

BLAST of Lsi04G019600 vs. NCBI nr
Match: gi|659103028|ref|XP_008452436.1| (PREDICTED: cytochrome P450 CYP72A219-like [Cucumis melo])

HSP 1 Score: 660.6 bits (1703), Expect = 1.8e-186
Identity = 337/448 (75.22%), Postives = 361/448 (80.58%), Query Frame = 1

Query: 4   KYSFMWLGPIPRVHIMDPEQLKIVFSLINDFQKPT--INPLVRLLANGLANHEGPKWVKH 63
           K SFMW+GPIPRVHIMDPEQLK VFSLIND+QKPT  +NPL +LLA+GL NHEG KWVKH
Sbjct: 100 KNSFMWIGPIPRVHIMDPEQLKTVFSLINDYQKPTASLNPLAKLLADGLLNHEGHKWVKH 159

Query: 64  RKIINHAFHLQKLKDMVPAFYHSCNEMISKWESMVFVEGSCELDVMPYLQNMAADVISRT 123
           RKIIN AFHL+KLKDMVPAFYHSC+EMISKWESMV V+GSCELDVMPYLQNM ADVISRT
Sbjct: 160 RKIINPAFHLEKLKDMVPAFYHSCDEMISKWESMVSVQGSCELDVMPYLQNMTADVISRT 219

Query: 124 AFGR------------------------------WRFLPTKSNNKMKEISKEITTLILGI 183
           AFG                               WRFLPTKSNNKMK ISKEI+TLI+GI
Sbjct: 220 AFGSSYEKGKKIFKLQTELAEMVIRSTLGIYIPGWRFLPTKSNNKMKVISKEISTLIMGI 279

Query: 184 MNEREKSMKAGEVIQTDLLSILMESNLNEIKQHGNNKDIGISIEDVIAECKLFYIAGQET 243
           +NERE SMKAGE  QTDLLSILMESNLNEIKQHGNNKDIG+SIEDVI ECKLFYIAGQET
Sbjct: 280 INERETSMKAGEATQTDLLSILMESNLNEIKQHGNNKDIGMSIEDVIDECKLFYIAGQET 339

Query: 244 TATLLVWTMVLLSSYSEWQEQARAEVFEIFGNKKPDYDGLSRLKVFYLIYEIKCPSFDIK 303
           TATLL WTMVLLSSYSEWQE+ARAEVFEIFGNKKPDYDGLSRLKV  +            
Sbjct: 340 TATLLTWTMVLLSSYSEWQERARAEVFEIFGNKKPDYDGLSRLKVVTM------------ 399

Query: 304 QSYEKVLEESILNSLERLYPPLSMFGHMVKKETRLGNLTLPAGVMLGIPSVLIQHDPKSW 363
                     ILN + RLYPP+SMFG  +KKET+LG LTLPAGVMLG+P+VLIQ DP+ W
Sbjct: 400 ----------ILNEVLRLYPPVSMFGRFIKKETKLGKLTLPAGVMLGLPTVLIQCDPELW 459

Query: 364 GEDADEFKPERFFEGVSKATKNLSAFVPFGWGPRICIGLNFAMIEAKMALSIILQGFSFE 420
           GEDA EFKPERFFEGVSKATKN  AFVPFGWGPRICIGLNFAMIEAKM LS+ILQ FS E
Sbjct: 460 GEDAHEFKPERFFEGVSKATKNSGAFVPFGWGPRICIGLNFAMIEAKMTLSMILQRFSLE 519

BLAST of Lsi04G019600 vs. NCBI nr
Match: gi|778695565|ref|XP_004146005.2| (PREDICTED: cytochrome P450 CYP72A219 isoform X1 [Cucumis sativus])

HSP 1 Score: 651.7 bits (1680), Expect = 8.4e-184
Identity = 334/448 (74.55%), Postives = 359/448 (80.13%), Query Frame = 1

Query: 4   KYSFMWLGPIPRVHIMDPEQLKIVFSLINDFQKPT--INPLVRLLANGLANHEGPKWVKH 63
           K SFMW+GP+PRVHIMDPEQLK VFSLIND+QKPT  +NPL +LLA+GL NHEG KWVKH
Sbjct: 101 KNSFMWIGPMPRVHIMDPEQLKTVFSLINDYQKPTASLNPLAKLLADGLLNHEGQKWVKH 160

Query: 64  RKIINHAFHLQKLKDMVPAFYHSCNEMISKWESMVFVEGSCELDVMPYLQNMAADVISRT 123
           RKIIN AFHL+KLKDMVPAFYHSC+EMISKWESMV VEG CELDVMPYLQNM ADVISRT
Sbjct: 161 RKIINPAFHLEKLKDMVPAFYHSCDEMISKWESMVSVEGFCELDVMPYLQNMTADVISRT 220

Query: 124 AFGR------------------------------WRFLPTKSNNKMKEISKEITTLILGI 183
           AFG                               WRFLPTKSNNKMK ISKEI+TLI+ I
Sbjct: 221 AFGSSYEKGKKIFKLQTELGEMVMQSTLGIYIPGWRFLPTKSNNKMKVISKEISTLIMDI 280

Query: 184 MNEREKSMKAGEVIQTDLLSILMESNLNEIKQHGNNKDIGISIEDVIAECKLFYIAGQET 243
           +NERE SMK+GE IQTDLLSILMESNLNEIKQHGNNKDIG+SIEDVI ECKLFYIAGQET
Sbjct: 281 INERETSMKSGEAIQTDLLSILMESNLNEIKQHGNNKDIGMSIEDVIDECKLFYIAGQET 340

Query: 244 TATLLVWTMVLLSSYSEWQEQARAEVFEIFGNKKPDYDGLSRLKVFYLIYEIKCPSFDIK 303
           TATLL WTMVLLSSYSEWQE+ARAEVFEIFGNKKPDYDGLSRLKV  +            
Sbjct: 341 TATLLTWTMVLLSSYSEWQERARAEVFEIFGNKKPDYDGLSRLKVVTM------------ 400

Query: 304 QSYEKVLEESILNSLERLYPPLSMFGHMVKKETRLGNLTLPAGVMLGIPSVLIQHDPKSW 363
                     ILN + RLYPP+SMFG +VKKET+LG LTLPAGVMLG+P VL+Q DP+ W
Sbjct: 401 ----------ILNEVLRLYPPVSMFGRLVKKETKLGKLTLPAGVMLGLPVVLMQCDPELW 460

Query: 364 GEDADEFKPERFFEGVSKATKNLSAFVPFGWGPRICIGLNFAMIEAKMALSIILQGFSFE 420
           GEDA EFKPERF EGVSKA KN  AFVPFGWGPRICIGLNFAMIEAKM LS+ILQ FS E
Sbjct: 461 GEDAHEFKPERFSEGVSKAAKNPGAFVPFGWGPRICIGLNFAMIEAKMTLSMILQRFSLE 520

BLAST of Lsi04G019600 vs. NCBI nr
Match: gi|778695569|ref|XP_011654017.1| (PREDICTED: cytochrome P450 CYP72A219 isoform X2 [Cucumis sativus])

HSP 1 Score: 650.2 bits (1676), Expect = 2.5e-183
Identity = 333/448 (74.33%), Postives = 358/448 (79.91%), Query Frame = 1

Query: 4   KYSFMWLGPIPRVHIMDPEQLKIVFSLINDFQKPT--INPLVRLLANGLANHEGPKWVKH 63
           K SFMW+GP+PRVHIMDPEQLK VFSLIND+QKPT  +NPL +LLA+GL NHEG KWVKH
Sbjct: 101 KNSFMWIGPMPRVHIMDPEQLKTVFSLINDYQKPTASLNPLAKLLADGLLNHEGQKWVKH 160

Query: 64  RKIINHAFHLQKLKDMVPAFYHSCNEMISKWESMVFVEGSCELDVMPYLQNMAADVISRT 123
           RKIIN AFHL+KLKDMVPAFYHSC+EMISKWESMV VEG CELDVMPYLQNM ADVISRT
Sbjct: 161 RKIINPAFHLEKLKDMVPAFYHSCDEMISKWESMVSVEGFCELDVMPYLQNMTADVISRT 220

Query: 124 AFGR------------------------------WRFLPTKSNNKMKEISKEITTLILGI 183
           AFG                               WRFLPTKSNNKMK ISKEI+TLI+ I
Sbjct: 221 AFGSSYEKGKKIFKLQTELGEMVMQSTLGIYIPGWRFLPTKSNNKMKVISKEISTLIMDI 280

Query: 184 MNEREKSMKAGEVIQTDLLSILMESNLNEIKQHGNNKDIGISIEDVIAECKLFYIAGQET 243
           +NERE SMK+GE IQTDLLSILMESNLNEIKQHGNNKDIG+SIEDVI ECKLFYIAGQET
Sbjct: 281 INERETSMKSGEAIQTDLLSILMESNLNEIKQHGNNKDIGMSIEDVIDECKLFYIAGQET 340

Query: 244 TATLLVWTMVLLSSYSEWQEQARAEVFEIFGNKKPDYDGLSRLKVFYLIYEIKCPSFDIK 303
           TATLL WTMVLLSSYSEWQE+ARAEVFEIFGNKKPDYDGLSRLKV  +            
Sbjct: 341 TATLLTWTMVLLSSYSEWQERARAEVFEIFGNKKPDYDGLSRLKVVTM------------ 400

Query: 304 QSYEKVLEESILNSLERLYPPLSMFGHMVKKETRLGNLTLPAGVMLGIPSVLIQHDPKSW 363
                     ILN + RLYPP+SMFG +VKKET+LG LTLPAGVMLG+P VL+Q DP+ W
Sbjct: 401 ----------ILNEVLRLYPPVSMFGRLVKKETKLGKLTLPAGVMLGLPVVLMQCDPELW 460

Query: 364 GEDADEFKPERFFEGVSKATKNLSAFVPFGWGPRICIGLNFAMIEAKMALSIILQGFSFE 420
           GEDA EFKPERF EGVSKA KN  AFVPFGWGPRICIGLNFAMIEAKMALS+ILQ FSF+
Sbjct: 461 GEDAHEFKPERFSEGVSKAAKNPGAFVPFGWGPRICIGLNFAMIEAKMALSMILQRFSFQ 520

BLAST of Lsi04G019600 vs. NCBI nr
Match: gi|659103032|ref|XP_008452438.1| (PREDICTED: cytochrome P450 CYP72A219-like [Cucumis melo])

HSP 1 Score: 648.7 bits (1672), Expect = 7.1e-183
Identity = 331/447 (74.05%), Postives = 358/447 (80.09%), Query Frame = 1

Query: 4   KYSFMWLGPIPRVHIMDPEQLKIVFSLINDFQKPTINPLVRLLANGLANHEGPKWVKHRK 63
           K S MWLGP+PRVHIMDPEQLKIVFS INDFQKPT+NPL RLL  GLA+ EG KWVKHRK
Sbjct: 97  KKSMMWLGPMPRVHIMDPEQLKIVFSSINDFQKPTMNPLTRLLVQGLASLEGQKWVKHRK 156

Query: 64  IINHAFHLQKLKDMVPAFYHSC-NEMISKWESMVFVEGSCELDVMPYLQNMAADVISRTA 123
           IIN AFH++KLKDMVPAFYHSC NEM+SKWE MV  EGSCELDVMPYLQNMA+DVISRTA
Sbjct: 157 IINPAFHVEKLKDMVPAFYHSCYNEMVSKWEGMVSREGSCELDVMPYLQNMASDVISRTA 216

Query: 124 FGR------------------------------WRFLPTKSNNKMKEISKEITTLILGIM 183
           FG                               WRFLPTKSN KMKEISKEITTLILGIM
Sbjct: 217 FGSSYEKGKKIFELQTELANLVIQANLGIYIPGWRFLPTKSNRKMKEISKEITTLILGIM 276

Query: 184 NEREKSMKAGEVIQTDLLSILMESNLNEIKQHGNNKDIGISIEDVIAECKLFYIAGQETT 243
           NE+EKS+KAGE IQTDLLSILMESN+NEIKQHGNNK+IG+SIEDVI ECKLFYIAGQETT
Sbjct: 277 NEKEKSIKAGEAIQTDLLSILMESNMNEIKQHGNNKNIGMSIEDVIEECKLFYIAGQETT 336

Query: 244 ATLLVWTMVLLSSYSEWQEQARAEVFEIFGNKKPDYDGLSRLKVFYLIYEIKCPSFDIKQ 303
           ATLLVWTMVLLSSYSEWQE+ARAEVFEIFGNKKPDYDGL+RLKV  +I+           
Sbjct: 337 ATLLVWTMVLLSSYSEWQERARAEVFEIFGNKKPDYDGLNRLKVVTMIF----------- 396

Query: 304 SYEKVLEESILNSLERLYPPLSMFGHMVKKETRLGNLTLPAGVMLGIPSVLIQHDPKSWG 363
                      N + RLYPP++MFG +++KETRLGNLTLP GVMLG+P VLI  DP+ WG
Sbjct: 397 -----------NEVLRLYPPVNMFGRIIRKETRLGNLTLPEGVMLGLPVVLIHLDPELWG 456

Query: 364 EDADEFKPERFFEGVSKATKNLSAFVPFGWGPRICIGLNFAMIEAKMALSIILQGFSFEL 420
           EDA EF PERF EG+SKATKN SAF+PFGWGPRICIG NFAMIEAKMALSIILQ FSFEL
Sbjct: 457 EDAHEFNPERFSEGISKATKNPSAFIPFGWGPRICIGQNFAMIEAKMALSIILQRFSFEL 516

BLAST of Lsi04G019600 vs. NCBI nr
Match: gi|778695562|ref|XP_011654016.1| (PREDICTED: cytochrome P450 CYP72A219-like [Cucumis sativus])

HSP 1 Score: 637.1 bits (1642), Expect = 2.1e-179
Identity = 323/446 (72.42%), Postives = 352/446 (78.92%), Query Frame = 1

Query: 4   KYSFMWLGPIPRVHIMDPEQLKIVFSLINDFQKPTINPLVRLLANGLANHEGPKWVKHRK 63
           K SFMWLGPIPRVHIMDPEQLK VFS  NDFQKPT+NP  +LL  G+ N +G KWVKHRK
Sbjct: 93  KNSFMWLGPIPRVHIMDPEQLKTVFSFYNDFQKPTMNPFTKLLVQGIVNLDGEKWVKHRK 152

Query: 64  IINHAFHLQKLKDMVPAFYHSCNEMISKWESMVFVEGSCELDVMPYLQNMAADVISRTAF 123
           IIN AFHL+KLKDMVPAFYHSCNEM+SKWESMV  EGSCELDVMPYL+NMA+DVISRTAF
Sbjct: 153 IINPAFHLEKLKDMVPAFYHSCNEMVSKWESMVSKEGSCELDVMPYLKNMASDVISRTAF 212

Query: 124 GR------------------------------WRFLPTKSNNKMKEISKEITTLILGIMN 183
           G                               WRFLPTK N KMKEISKEITTLILGIM+
Sbjct: 213 GSSYEKGKKIFKLQTELTNFVIQTTLGIYIPGWRFLPTKLNRKMKEISKEITTLILGIMD 272

Query: 184 EREKSMKAGEVIQTDLLSILMESNLNEIKQHGNNKDIGISIEDVIAECKLFYIAGQETTA 243
           EREKSMKAGE IQTDLLSILMESN+NEIKQHGN KDIG+SIEDVI ECKLFYIAGQETTA
Sbjct: 273 EREKSMKAGEAIQTDLLSILMESNMNEIKQHGNKKDIGMSIEDVIEECKLFYIAGQETTA 332

Query: 244 TLLVWTMVLLSSYSEWQEQARAEVFEIFGNKKPDYDGLSRLKVFYLIYEIKCPSFDIKQS 303
           TLLVWTM+LLSSYSEWQE+ARAEVFEIFGNK+P+YDGL+RLKV  +I+            
Sbjct: 333 TLLVWTMILLSSYSEWQERARAEVFEIFGNKQPNYDGLNRLKVVTMIF------------ 392

Query: 304 YEKVLEESILNSLERLYPPLSMFGHMVKKETRLGNLTLPAGVMLGIPSVLIQHDPKSWGE 363
                     N + RLYPP S+F  +V+KETRLGNLTLP GVMLG+P VLIQ DP+ WGE
Sbjct: 393 ----------NEVLRLYPPGSLFVRIVRKETRLGNLTLPGGVMLGLPIVLIQRDPELWGE 452

Query: 364 DADEFKPERFFEGVSKATKNLSAFVPFGWGPRICIGLNFAMIEAKMALSIILQGFSFELS 420
           DA EF PERF  GVSKATKN SAF+PFGWGPRICIG  FAMIEAKMALS+ILQ FSFELS
Sbjct: 453 DAHEFNPERFSGGVSKATKNPSAFIPFGWGPRICIGQTFAMIEAKMALSMILQRFSFELS 512

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
C7A29_PANGI1.8e-12551.45Cytochrome P450 CYP72A219 OS=Panax ginseng PE=2 SV=1[more]
C7263_MEDTR1.3e-12350.0011-oxo-beta-amyrin 30-oxidase OS=Medicago truncatula GN=CYP72A63 PE=1 SV=1[more]
C7254_GLYUR6.4e-12349.3311-oxo-beta-amyrin 30-oxidase OS=Glycyrrhiza uralensis GN=CYP72A154 PE=1 SV=1[more]
C7A15_ARATH2.5e-11949.21Cytochrome P450 72A15 OS=Arabidopsis thaliana GN=CYP72A15 PE=2 SV=1[more]
C7A13_ARATH7.3e-11949.55Cytochrome P450 72A13 OS=Arabidopsis thaliana GN=CYP72A13 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
W9QZH6_9ROSA8.9e-14056.85Secologanin synthase OS=Morus notabilis GN=L484_024637 PE=3 SV=1[more]
I1MIY4_SOYBN1.9e-13754.91Uncharacterized protein OS=Glycine max GN=GLYMA_15G244200 PE=3 SV=1[more]
A0A0B2SBC8_GLYSO1.2e-13654.46Secologanin synthase OS=Glycine soja GN=glysoja_039974 PE=3 SV=1[more]
I1MIY5_SOYBN1.2e-13654.46Uncharacterized protein OS=Glycine max GN=GLYMA_15G244300 PE=3 SV=1[more]
M5WPE9_PRUPE1.6e-13655.28Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004271mg PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G14610.14.2e-12550.45 cytochrome P450, family 72, subfamily A, polypeptide 7[more]
AT3G14690.11.4e-12049.21 cytochrome P450, family 72, subfamily A, polypeptide 15[more]
AT3G14660.14.1e-12049.55 cytochrome P450, family 72, subfamily A, polypeptide 13[more]
AT3G14630.15.0e-11849.11 cytochrome P450, family 72, subfamily A, polypeptide 9[more]
AT3G14680.16.5e-11848.42 cytochrome P450, family 72, subfamily A, polypeptide 14[more]
Match NameE-valueIdentityDescription
gi|659103028|ref|XP_008452436.1|1.8e-18675.22PREDICTED: cytochrome P450 CYP72A219-like [Cucumis melo][more]
gi|778695565|ref|XP_004146005.2|8.4e-18474.55PREDICTED: cytochrome P450 CYP72A219 isoform X1 [Cucumis sativus][more]
gi|778695569|ref|XP_011654017.1|2.5e-18374.33PREDICTED: cytochrome P450 CYP72A219 isoform X2 [Cucumis sativus][more]
gi|659103032|ref|XP_008452438.1|7.1e-18374.05PREDICTED: cytochrome P450 CYP72A219-like [Cucumis melo][more]
gi|778695562|ref|XP_011654016.1|2.1e-17972.42PREDICTED: cytochrome P450 CYP72A219-like [Cucumis sativus][more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: Molecular Function
TermDefinition
GO:0020037heme binding
GO:0005506iron ion binding
GO:0016705oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
Vocabulary: INTERPRO
TermDefinition
IPR017972Cyt_P450_CS
IPR002401Cyt_P450_E_grp-I
IPR001128Cyt_P450
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G019600.1Lsi04G019600.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 367..378
score: 2.5E-11coord: 358..367
score: 2.5E-11coord: 207..224
score: 2.5E-11coord: 281..292
score: 2.5
IPR001128Cytochrome P450GENE3DG3DSA:1.10.630.10coord: 8..418
score: 1.0
IPR001128Cytochrome P450PFAMPF00067p450coord: 8..125
score: 2.7E-12coord: 127..255
score: 5.2E-17coord: 288..394
score: 2.0
IPR001128Cytochrome P450unknownSSF48264Cytochrome P450coord: 6..417
score: 1.2
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 357..367
score: 3.0E-15coord: 321..345
score: 3.0E-15coord: 216..242
score: 3.0E-15coord: 196..213
score: 3.0E-15coord: 367..390
score: 3.0
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 360..369
scor
NoneNo IPR availablePANTHERPTHR24282CYTOCHROME P450 FAMILY MEMBERcoord: 4..258
score: 1.8E-232coord: 281..419
score: 1.8E
NoneNo IPR availablePANTHERPTHR24282:SF53CYTOCHROME P450-RELATEDcoord: 4..258
score: 1.8E-232coord: 281..419
score: 1.8E